Integration analysis of transcriptome and proteome of Chinese Merino sheep (Ovis aries) embryonic skeletal muscle

Mian Feng, Wenping Hu, Xinyue Wang, Lulu Liu, Yunhui Liu, Li Zhang
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Abstract

Abstract The growth and development of sheep late fetal skeletal muscle undergoes significant changes. However, the specific mechanism remains unknown. In this study, we performed the comprehensive analysis of transcriptome and proteome of Chinese Merino sheep at embryonic ages Day85 (D85N), Day105 (D105N), and Day135 (D135N) by the tandem mass tags (TMT) and RNA‐seq methods. Totally 717, 1253, and 1873 differentially expressed genes (DEGs) were identified in the three comparison groups (D85N vs. D105N, D105N vs. D135N, and D85N vs. D135N). Among which 7, 80, and 162 DEGs were identified with the same trends at mRNA and protein levels in the three groups. Enrichment analysis showed that 7 genes with same trends in D85 vs. D105 have not been enriched in any pathways, which indicated that the development of skeletal muscle underwent significant changes with post‐transcription regulation during this period. These genes with same trends in D105N vs. D135N were mainly enriched in the pathways related to skeletal muscle metabolism and maturation, including oxidative phosphorylation, glycolysis/gluconeogenesis, tight junction, and HIF‐1 pathways, which indicated that the development of skeletal muscle tended to maturation during this period. These results provided evidence for ovine late fetal skeletal muscle fibers development from proliferating to thickening at simultaneous transcriptional and translational levels.
中国美利奴羊胚胎骨骼肌转录组和蛋白质组的整合分析
绵羊晚期胎儿骨骼肌的生长发育发生了显著变化。然而,具体的机制尚不清楚。本研究采用串联质量标签(TMT)和RNA‐seq方法,对中国美利奴绵羊Day85 (D85N)、Day105 (D105N)和Day135 (D135N)胚胎龄的转录组和蛋白质组进行了综合分析。在D85N与D105N、D105N与D135N、D85N与D135N三个对照组中,共鉴定出717、1253和1873个差异表达基因(deg)。其中7个、80个和162个基因在mRNA和蛋白水平上具有相同的变化趋势。富集分析显示,在D85和D105中具有相同趋势的7个基因没有在任何途径中富集,这表明在这一时期骨骼肌的发育在转录后调控下发生了显著变化。这些与D105N和D135N趋势相同的基因主要富集在与骨骼肌代谢和成熟相关的途径中,包括氧化磷酸化、糖酵解/糖异生、紧密连接和HIF‐1途径,这表明骨骼肌的发育在这一时期趋于成熟。这些结果为羊晚期胎儿骨骼肌纤维从增殖到增厚的发育提供了转录和翻译水平同步发生的证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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