Elsevier最新文献_第4页

An "on-off" electrochemical immunosensor for the detection of the glycan antigen CA125 by amplification signals using electropositive COFs. 一种“开-关”电化学免疫传感器，通过使用电正COFs的放大信号来检测多糖抗原CA125。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-13 DOI: 10.1016/j.talanta.2025.127593

Wenxiao Jin, Rongfang Chen, Likang Wu, Canwei Peng, Yonghai Song, Longfei Miao, Li Wang

{"title":"An \"on-off\" electrochemical immunosensor for the detection of the glycan antigen CA125 by amplification signals using electropositive COFs.","authors":"Wenxiao Jin, Rongfang Chen, Likang Wu, Canwei Peng, Yonghai Song, Longfei Miao, Li Wang","doi":"10.1016/j.talanta.2025.127593","DOIUrl":"10.1016/j.talanta.2025.127593","url":null,"abstract":"Cancer Antigen 125 (CA125), is a high molecular weight mucinous glycoprotein found on the surface of ovarian cancer cells. Generally, 90 % of women may appear a high concentration of CA125 when they got the cancer; thus, CA125 can act as a marker for ovarian cancer diagnosis and therapeutic evaluation. COFs have been widely used for disease detection due to their structural stability, high loading capacity and biocompatibility. However, the limited variety of electroactive COFs used as signal probes, fewer enriched signaling molecules, weaker electrical signals generated, and higher oxidation or reduction potentials of electroactive substances, a series of side reactions are easily triggered causing serious interference. To solve the above problems, [Fe(CN)6]3/4- as a signal probe and COFs for signal amplification were selected to creating a highly sensitive electrochemical immunosensor for glycan antigen CA125. Firstly, two-dimensional (2D) EP-TD-COF with ultra-high specific surface area was modified on bare GCE, which could covalently bound numerous Ab1 molecules due to the epoxy-rich functional groups. Then, the electropositive AuNPs@2DCOFBTT-DGMH was prepared by the in situ growth of AuNPs, proved an effective platform for loading Ab2 molecules via Au-S bonds. Based on the positively charged AuNPs@COFBTT-DGMH/Ab2 and negatively charged [Fe(CN)6]3/4- of electrostatic interactions, which could significantly enchaned signal for quantitative and sensitive detection of CA125. The constructed immunosensor exhibits excellent stability performance and high sensitivity, enabling ultrasensitive detection of trace glycan antigens. This study provided a new idea for the use of non-electroactive substances for the construction of electrochemical immunosensors and provided an effective signal amplification strategy.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127593"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First wheat certified reference material for organically bound tritium measurement in the environment. 首个小麦认证的环境中有机结合氚测量标准物质。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-02 DOI: 10.1016/j.talanta.2025.127515

Marielle Crozet, Audrey Bacchetta, Nicolas Baglan, Claire Dalencourt, Yvan Losset, Aurélie Ritt, Véronique Labed, Giacomo Canciani, Sébastien Picart, Cédric Rivier

引用次数: 0

Lateral flow assay with automatic signal amplification based on delayed substrate release. 基于延迟底物释放的自动信号放大横向流动试验。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-13 DOI: 10.1016/j.talanta.2025.127557

Yue Sun, Minxin Mao, Shengmei Tai, Mengjia Chao, Hengyu Xu, Yina Cai, Chifang Peng, Wei Ma, Zhouping Wang

{"title":"Lateral flow assay with automatic signal amplification based on delayed substrate release.","authors":"Yue Sun, Minxin Mao, Shengmei Tai, Mengjia Chao, Hengyu Xu, Yina Cai, Chifang Peng, Wei Ma, Zhouping Wang","doi":"10.1016/j.talanta.2025.127557","DOIUrl":"10.1016/j.talanta.2025.127557","url":null,"abstract":"The low sensitivity of Lateral flow assay (LFA) limits its application in rapid detection for trace targets. LFAs with nanozyme (nanozyme-LFA) as signal labels have demonstrated excellent performance in point of care testing (POCT). However, additional operational steps for substrate catalysis in nanozyme LFA are required, which makes the nanozyme-LFA operation complicated. In this work, we designed a LFA based on delayed substrate release (SGF-LFA), in which a commercialized glass fiber membrane embedded with substrate (SGF) was fixed at the sample pad. The SGF could automatically execute substrate delivery and catalysis, thus eventually achieving a one-step LFA operation for the nucleic acid detection of influenza A virus H1N1. In this SGF-LFA, 3,3 '- diaminobenzidine (DAB) was oxidized and deposited, producing a strong signal amplification under the catalysis of Au@PtNP nanozyme. The SGF-LFA could detect the nucleic acid of H1N1, with a linear range of 0.02-50 nM and a limit of detection (LOD) as low as 0.02 nM, which was 25-fold lower than that of the nanozyme-LFA before catalysis. In addition, the analytical performance was close to that of a manual operation mode of catalysis amplification. The application of SGF-LFA for detecting the H1N1 nucleic acid in serum samples obtained a recovery rate of 96 %-102.7 %, indicating that SGF-LFA has great potential in point-of-care testing.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127557"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel multiparameter optical sensor head design for marine environments. 海洋环境下新型多参数光学传感器头设计。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-10 DOI: 10.1016/j.talanta.2025.127571

Sean Power, Louis Free, Ciprian Briciu-Burghina, Chloe Richards, Adrian Delgado, Elena Gomez-Alvarez, Nigel Kent, Fiona Regan

{"title":"Novel multiparameter optical sensor head design for marine environments.","authors":"Sean Power, Louis Free, Ciprian Briciu-Burghina, Chloe Richards, Adrian Delgado, Elena Gomez-Alvarez, Nigel Kent, Fiona Regan","doi":"10.1016/j.talanta.2025.127571","DOIUrl":"10.1016/j.talanta.2025.127571","url":null,"abstract":"Anthropogenic activities have led to increased stress on our marine and other aquatic environments. There is a pressing need to monitor, measure, understand and mitigate causes of these pressures. This paper presents a novel optical head for monitoring and measuring marine based optical phenomena. The development and preliminary testing of the optical head were designed to detect optically active constituents in the marine and coastal environments. Potential applications may include the detection of Harmful Algal Blooms (HAB), which due to their production of toxins have deleterious effects on marine ecosystems, dissolved organic matter (DOM), oil spills, through the measurement of dissolved fluorescent petroleum compounds and turbidity, a key metric in marine and water quality measurements. Preliminary laboratory based results indicate that the optical head is well suited for measuring in-vivo Chlorophyll a (Chl a) fluorescence, turbidity, fluorescent dissolved organic matter (fDOM) and petroleum. For turbidity and in-vivo Chl a, analytical performance was benchmarked against off-the-shelf commercial sensors. The developed optical head demonstrates good analytical performance with certified reference standards and a very good agreement with the reference instrument.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127571"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cucurbit[6]uril host-guest interaction assisted N-terminal epitope imprinted particles for cytochrome c recognition prepared by reversible addition-fragmentation chain transfer strategy. 采用可逆加成-断裂链转移策略制备了用于细胞色素c识别的瓜b[6] - uril主客体相互作用辅助n端表位印迹颗粒。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-10 DOI: 10.1016/j.talanta.2025.127567

Nurimangul Muntiza, Wenbin Zhang, Yuzeng Li, Wenquan Ji, Jin Zhao, Hongfeng Zhang, Qinran Li, Qiliang Deng, Donglan Sun, Tianjun Liu

{"title":"Cucurbit[6]uril host-guest interaction assisted N-terminal epitope imprinted particles for cytochrome c recognition prepared by reversible addition-fragmentation chain transfer strategy.","authors":"Nurimangul Muntiza, Wenbin Zhang, Yuzeng Li, Wenquan Ji, Jin Zhao, Hongfeng Zhang, Qinran Li, Qiliang Deng, Donglan Sun, Tianjun Liu","doi":"10.1016/j.talanta.2025.127567","DOIUrl":"10.1016/j.talanta.2025.127567","url":null,"abstract":"A novel strategy for cytochrome c selective recognition assisted with cucurbit[6]uril by host-guest interaction via N-terminal epitope imprinting and reversible addition-fragmentation chain transfer (RAFT) polymerization was developed. N-terminal nonapeptide of cytochrome c (GI-9) was used as the epitope template to achieve highly selective recognition of cytochrome c. As a common supramolecule in recent years, cucurbit[6]uril can encapsulate the butyrammonium group of lysine residue to capture the peptide and improve the corresponding spatial orientation by the host-guest interaction for GI-9 or cytochrome c recognition. After cucurbit[6]uril modification and epitope immobilization, the imprinted polymer was synthesized by RAFT polymerization with 2-dodecylsulfanylcarbothioylsulfanyl-2-methylpropanoic acid as chain transfer agent. After template removal, the obtained imprinted particles showed good binding ability to GI-9 (20.28 mg g-1, IF = 4.11) and cytochrome c (36.12 mg g-1, IF = 3.91). With the successive addition of cucurbit[6]uril and RAFT agent, the step-by-step improvement of the IF for cytochrome c recognition further illustrated the effects of supramolecular host-guest interaction and regulation of imprinted polymer chain. The imprinted polymers showed obvious advantages for cytochrome c recognition compared to competitive proteins and had good reusability with the repeated reproduction rate 80.8 % after five cycles of adsorption and desorption. Furthermore, the selective recognition for cytochrome c in adult bovine serum proved its potentiality to be applied in practical samples. All these results demonstrated that the combination of epitope imprinting, cucurbit[6]uril host-guest interaction and RAFT strategy presented an efficient new feasible control method for protein recognition with good selectivity, stability and reusability.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127567"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxygen vacancy and interface effect dual modulation of SnS₂/SnO₂ heterojunction for boosting formaldehyde detection at low temperature. 氧空位和界面效应双调制SnS2/SnO2异质结促进甲醛低温检测。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-13 DOI: 10.1016/j.talanta.2025.127586

Dan Meng, Li Ma, Lei Zhang, Xiaoguang San, Zongsheng Xie, Quan Jin, Jian Qi

{"title":"Oxygen vacancy and interface effect dual modulation of SnS2/SnO2 heterojunction for boosting formaldehyde detection at low temperature.","authors":"Dan Meng, Li Ma, Lei Zhang, Xiaoguang San, Zongsheng Xie, Quan Jin, Jian Qi","doi":"10.1016/j.talanta.2025.127586","DOIUrl":"10.1016/j.talanta.2025.127586","url":null,"abstract":"Formaldehyde (HCHO) is a harmful volatile organic pollutant, which is commonly found in interior decoration and furniture products. Therefore, it is necessary to develop a gas sensor that can quickly and accurately detect formaldehyde for human health and environmental protection. In order to achieve this goal, in this work, SnS2/SnO2 heterostructure was synthesized by in-situ sulfurization process on the basis of SnO2 nanospheres, and its formaldehyde sensing performance was studied. After testing, it was found that the gas sensor based on SnS2/SnO2 heterojunction has more excellent gas sensing performance than pure SnO2 gas sensor at the same operating temperature (100 °C). Specifically, SnS2/SnO2-2 (Sn:S = 3:2) has the advantages of high sensitivity (4.01 at 0.1 ppm), excellent selectivity, low theoretical detection limit (13.26 ppb), good humidity resistance and long-term stability. The excellent sensing performance of SnS2/SnO2 sensors for formaldehyde detection is mainly attributed to the n-n heterojunction formed by SnS2 and SnO2, which generates a built-in electric field to accelerate the electron transport in the material, the higher oxygen vacancy sites adsorb a large number of reactive gas molecules to promote the oxidation of formaldehyde molecules, and the unique porous structure to promote the transmission and diffusion of gases and increase the surface area to provide more adsorption sites and reactive centers for gas molecules. Therefore, the construction of SnS2/SnO2 heterostructures will be an effective way to develop next-generation formaldehyde gas sensors with higher sensing performance.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127586"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of glucose detection using 10 nm Al₂O₃ thin film on diamond solution-gate field-effect transistor. 金刚石溶液栅场效应晶体管上10nm Al2O3薄膜对葡萄糖检测的改进。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-08 DOI: 10.1016/j.talanta.2025.127560

Yuxiang Du, Qianwen Zhang, Huaxiong Wu, Xiaohuan Liu, Genqiang Chen, Yuesong Liang, Qi Li, Yangxin Gu, Minghui Zhang, Hongxing Wang

{"title":"Improvement of glucose detection using 10 nm Al2O3 thin film on diamond solution-gate field-effect transistor.","authors":"Yuxiang Du, Qianwen Zhang, Huaxiong Wu, Xiaohuan Liu, Genqiang Chen, Yuesong Liang, Qi Li, Yangxin Gu, Minghui Zhang, Hongxing Wang","doi":"10.1016/j.talanta.2025.127560","DOIUrl":"10.1016/j.talanta.2025.127560","url":null,"abstract":"Glucose detection is crucial for diagnosis, prevention and treatment of diabetes mellitus. In this work, 10 nm Al2O3 thin film was introduced on the channel of diamond solution-gate field-effect transistor (SGFET) to improve the performance of glucose detection. AFM results show the roughness of channel surface increased after Al2O3 thin film deposition. Then, 1-pyrenebutyric acid-N-hydroxy succinimide ester (Pyr-NHS) and glucose oxidase (GOD) were linked on the channel. The morphology after each modification step was evaluated by SEM, and the result indicated an uneven Al2O3 distribution. XPS spectra further confirmed the effective modification of Pyr-NHS and GOD. In addition, the shifts of transfer characteristics for each concentration of glucose were analyzed, which illustrated a wide linear response (10-8-10-2 M), a high sensitivity (-44.01 mV/log10[glucose concentration]) and a low detection limitation (10-8 M). All these results show an excellent detection performance, which may provide a new idea for the design of diamond SGFET biosensor.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127560"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amplified electrochemiluminescence of Ru(dcbpy)₃²⁺ via coreactant active sites on nitrogen-doped graphene quantum dots. 氮掺杂石墨烯量子点上Ru(dcbpy)32+通过反应物活性位点的放大电化学发光。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-13 DOI: 10.1016/j.talanta.2025.127554

Lingyue Guo, Libo Li, Lijun Luo, Tianyan You

{"title":"Amplified electrochemiluminescence of Ru(dcbpy)32+ via coreactant active sites on nitrogen-doped graphene quantum dots.","authors":"Lingyue Guo, Libo Li, Lijun Luo, Tianyan You","doi":"10.1016/j.talanta.2025.127554","DOIUrl":"10.1016/j.talanta.2025.127554","url":null,"abstract":"Searching for new alternative to tripropylamine (TPrA) with low toxicity and high chemical stability for the tris(4,4'-dicarboxylic acid-2,2'-bipyridyl)ruthenium (II) (Ru(dcbpy)32+) based coreactant electrochemiluminescence (ECL) system is essential for widespread analytical applications. Here, nitrogen-doped graphene quantum dots (NGQDs) have been discovered to significantly amplify the ECL emission and increase the ECL efficiency of Ru(dcbpy)32+ for the first time. However, the mechanism by which NGQDs act as coreactants is not well comprehended. Therefore, various optical and electrochemical technologies were employed to investigate the ECL mechanism. It is proposed that the amino and carboxyl groups on the surface of NGQDs play crucial roles as the coreactant active sites, catalyzing the oxidation of Ru(dcbpy)32+. Based on this foundation, an \"on-off-on\" ECL aptasensor for the quantification of acetamiprid was developed, exhibiting a broad linear range and a detection limit of 0.056 pM. Satisfactory recoveries, ranging from 98.0 % to 101.6 %, were achieved in pakchoi samples. Consequently, NGQDs could serve as coreactants for Ru(dcbpy)32+, offering new opportunities for constructing a variety of sensors with extensive analytical applications in the ECL field.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127554"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sea urchin-like covalent organic frameworks/TiO₂ heterostructure for enhanced photocatalytic CO₂ conversion.

IF 9.4 1区化学

Journal of Colloid and Interface Science Pub Date : 2025-05-01 Epub Date: 2025-01-27 DOI: 10.1016/j.jcis.2025.01.231

Xin Zhao, Qianxi Liu, Qi Li, Yihang Yin, Mang Zheng, Fanqi Luo, Huiquan Gu, Baojiang Jiang

{"title":"Sea urchin-like covalent organic frameworks/TiO2 heterostructure for enhanced photocatalytic CO2 conversion.","authors":"Xin Zhao, Qianxi Liu, Qi Li, Yihang Yin, Mang Zheng, Fanqi Luo, Huiquan Gu, Baojiang Jiang","doi":"10.1016/j.jcis.2025.01.231","DOIUrl":"10.1016/j.jcis.2025.01.231","url":null,"abstract":"Photocatalytic reduction of CO2 to valuable chemicals is an effective strategy to address the environmental problems and energy crisis. Covalent organic frameworks (COFs) are emerging materials known for their excellent diverse properties, albeit limited by special synthetic methods, including high temperature (120 °C) and the necessity of inert gas atmosphere. Herein, a novel synthesis method under room temperature and air was optimized to form TpPa-COF (TP-COF) by p-phenylenediamine (Pa) and 2,4,6-triformyl phloroglucinol (Tp) through electrostatic self-assembly. To further expand the application scope of TP-COF, a heterojunction structure was constructed by in-situ growth of TP-COF onto TiO2 to form TiO2@TP-COF. In the photocatalytic CO2 reaction of TiO2@TP-COF composites, TiO2 acts as a reduction site to reduce CO2 to CO, and triethanolamine (TEOA) acts as a hole-sacrificing reagent. It was demonstrated by in situ X-ray photoelectron spectroscopy (XPS) that the direction of electron transfer in the TiO2@TP-COF composites flowed from TP-COF to TiO2. Meanwhile, TEOA on TP-COF was oxidized to consume holes and produce protons for the reduction of CO2. Combining the advantages of organic and inorganic semiconductors, the heterojunction structure effectively improves the photocatalytic properties of TiO2@TP-COF under visible light irradiation. TiO2@TP-COF demonstrates a remarkable photocatalytic CO2 reduction rate of 133.37 μmol/g/h at λ = 420 nm, which is 3.19 and 2.88 times higher than that of TP-COF and TiO2, respectively, while exhibiting a selectivity of 73 % for CO. This convenient method of synthesizing TiO2@TP-COF catalysts will open up new perspectives for future COF-based materials.","PeriodicalId":351,"journal":{"name":"Journal of Colloid and Interface Science","volume":"685 ","pages":"1068-1076"},"PeriodicalIF":9.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-molecule resolution of the conformation of polymers and dendrimers with solid-state nanopores. 具有固态纳米孔的聚合物和树状大分子构象的单分子分辨率。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-10 DOI: 10.1016/j.talanta.2025.127544

Meili Ren, Fupeng Qin, Yue Liu, Daixin Liu, Renata Pereira Lopes, Didier Astruc, Liyuan Liang

{"title":"Single-molecule resolution of the conformation of polymers and dendrimers with solid-state nanopores.","authors":"Meili Ren, Fupeng Qin, Yue Liu, Daixin Liu, Renata Pereira Lopes, Didier Astruc, Liyuan Liang","doi":"10.1016/j.talanta.2025.127544","DOIUrl":"10.1016/j.talanta.2025.127544","url":null,"abstract":"Polymers and dendrimers are macromolecules, possessing unique and intriguing characteristics, that are widely applied in self-assembled functional materials, green catalysis, drug delivery and sensing devices. Traditional approaches for the structural characterization of polymers and dendrimers involve DLS, GPC, NMR, IR and TG, which provide their physiochemical features and ensemble information, whereas their unimolecular conformation and dispersion also are key features allowing to understand their transporting profile in confined ionic nanochannels. This work demonstrates the nanopore approach for the determination of charged homopolymers, neutral block copolymer and dendrimers under distinct bias potentials and pH conditions. The nanopore translocation properties reveal that the dispersion and transporting of PEI is pH-dependent, and its capture rate is much lower than that of PAA. The neutral block copolymer with longest molecular chain threads through with longest blockage duration, its highest capture rate was achieved in 0.5 M KCl at pH 5 with slow diffusion and high temporal resolution. The two generations of neutral dendrimers could also translocate under bias potentials, probably due to the ions adsorption on the dendrimers and driven by Brownian force. The TEG-81 with larger molecular size translocates with longer residence time and higher blockage ratio, as expected. Both of the dendrimers exhibit a higher blockage ratio at pH 7.4 than either acidic or alkalic condition, indicating a larger stretched conformation adopted under neutral condition. This work presents the analysis of unimolecular charged and neutral polymers and dendrimers, which will be insightful in understanding the self-assembly motion and transfer of synthetic macromolecules in confined space. It also provides a good indication for deciphering the macromolecule-nanopore interplay under electrophoretic condition.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127544"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0