Elsevier最新文献

{"title":"Signal processing for miniature mass spectrometer based on LSTM-EEMD feature digging.","authors":"Chenrui Zhan, Zisheng Ju, Binrui Xie, Jiwen Chen, Qiang Ma, Ming Li","doi":"10.1016/j.talanta.2024.126904","DOIUrl":"10.1016/j.talanta.2024.126904","url":null,"abstract":"<p><p>Miniature mass spectrometers exhibit immense application potential in on-site detection due to their small size and low cost. However, their detection accuracy is severely affected by factors such as sample pre-processing and environmental conditions. In this study, we propose a data processing method based on long short-term memory-ensemble empirical mode decomposition (LSTM-EEMD) to improve the quality of on-site detection data from miniature mass spectrometers. The EEMD method can clearly decompose the different physical feature components in the small-scale spectrometer signals, while the LSTM method can adaptively learn the internal feature relationships of the signals. Thus, by combining the two, the parameters for the EEMD signal reconstruction can be optimized in an adaptive manner, obtaining the optimized coefficients. Compared to the previous EEMD feature enhancement approach, the LSTM-EEMD method not only significantly improves the coefficient of determination (R²) and relative standard deviation (RSD) of the data, enhancing the linear range, but also achieves fully adaptive processing throughout the workflow, greatly boosting the efficiency. By leveraging a miniature mass spectrometer, data for N-acetyl-l-aspartic acid (NAA), 2-Hydroxyglutarate (2-HG), and γ-Aminobutyric acid (GABA) in actual blood samples have been obtained. The experimental results demonstrate that the LSTM-EEMD method can markedly enhance the accuracy and usability of the biological sample data in practical testing, providing new perspectives and possibilities for research and applications in the relevant domain.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A one-pot isothermal Fluorogenic Mango II arrays-based assay for label-free detection of miRNA.","authors":"Zan Gong, Panpan Yuan, Yuqing Gan, Xi Long, Zhiwei Deng, Yalan Tang, Yanjing Yang, Shian Zhong","doi":"10.1016/j.talanta.2024.126920","DOIUrl":"10.1016/j.talanta.2024.126920","url":null,"abstract":"<p><p>The capability to detect a small number of miRNAs in clinical samples with simplicity, selectivity, and sensitivity is immensely valuable, yet it remains a daunting task. Here, we described a novel Mango II aptamers-based sensor for the one-pot, sensitive and specific detection of miRNAs. Target miRNA-initiated mediated catalyzed hairpin assembly (CHA) would allow for the production of plenty of DNA duplexes and the formation of the complete T7 promoter, motivating the rolling circle transcription (RCT). Then, the subsequent RCT process efficiently generates a huge number of repeating RNA Mango II aptamers, brightened by the incorporation of fluorescent dye TO1-B for miRNA quantification, realizing label-free and high signal-to-background ratio. Moreover, this assay possesses a remarkable ability to confer high selectivity, enabling the distinction of miRNAs among family members with mere 1- or 2- nucleotide (nt) differences. By employing the proposed assay, we have successfully achieved a sensitive evaluation of miR-21 content in diverse cell lines and clinical serum samples. This offers a versatile approach for the sensitive assay of miRNA biomarkers in molecular diagnosis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of 4-n-butylresorcinol by fluorescence derivatization based on dopamine.","authors":"Qing Liu, Si Chen, Yiming Nie, Qian Li, Fang Chen","doi":"10.1016/j.talanta.2024.126909","DOIUrl":"10.1016/j.talanta.2024.126909","url":null,"abstract":"<p><p>4-n-butylresorcinol (4nBR) is a frequently utilized as whitening ingredients in skincare cosmetics. Compared with other whitening ingredients, it can effectively inhibit tyrosinase with lower toxicity and superior inhibition efficacy. Under alkaline conditions, an induced oxidative coupling reaction can occur between 4nBR and dopamine (DA) to generate strong fluorescent substance azamonardine with an intense emission band centering at 476 nm when excited at 440 nm. This phenomenon can be used to establish a fluorescence analysis method for 4nBR. The results indicated that the linear range of the method was 1.0-24.0 nmol L^-1, and the detection limit was as low as 0.25 nmol L^-1. The method showed high sensitivity, good selectivity, mild experimental conditions and low cost. The proposed method was successfully used to detect 4nBR in cosmetics, and the results were consistent with those of HPLC. The spiking recoveries were between 98.2% and 108 %.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valence fixable ferrozine gel rod combined with smartphone for facile determination of redox-active Fe²⁺ in environmental water.","authors":"Peng'an Zhu, Jiangle Zhang, Jingjing Jin, Xing Huang, Xinfeng Zhang","doi":"10.1016/j.talanta.2024.126933","DOIUrl":"10.1016/j.talanta.2024.126933","url":null,"abstract":"<p><p>Ferrous ion (Fe²⁺) can indicate the redox situation of water and also plays an important role in maintaining the ecological balance of water bodies. However, due to the redox-active property of Fe²⁺, it is still a huge challenge to sensitively and accurately determine Fe²⁺ especially in interstitial water. Herein, we prepared a ferrozine gel rod for valence fixation during sampling and subsequent smartphone-based detection of Fe²⁺. The electrode potential of the redox pair can be varied through the formation of Fe²⁺-ligand complexes, and when E_complex was higher than [Formula: see text] , the oxidation of Fe²⁺ by O₂ was hindered, thus achieving the valence fixation of Fe²⁺. Six ligands were screened, and it was found that ferrozine could effectively increase the redox potential after complexing with Fe²⁺, and also exhibits an obvious color change while fixing the valence of Fe²⁺. To facilitate Fe²⁺ detection, a cross-linked porous polymer gel rod prepared by acrylamide and sodium alginate was used to encapsulate the ferrozine molecules. The ferrozine gel rod enabled fixation the valence of Fe²⁺ longer than 30 days, and the resulted purple-red color was pictured and analyzed by a smartphone. Ultimately, the developed ferrozine gel rod sensing system was able to achieve sensitive and linear detection of Fe²⁺ in the range of 1-200 μM with the limit of detection as low as 0.33 μM, and it also exhibited excellent selectivity and anti-interference ability. The accuracy and reliability of the method was verified by the determination of Fe²⁺ in spiked water samples and certified standard reference water samples. Finally, the ferrozine gel rod sensing system was successfully applied to in-situ detection of Fe²⁺ in interstitial water, overlying water and upper water of lake and river. This facile system that enabled valence fixation and fast detection is promising for detection of Fe²⁺ in environmental waters.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effect of 2D covalent organic frameworks confined 0D carbon quantum dots film: Toward molecularly imprinted cathodic photoelectrochemical platform for detection of tetracycline.","authors":"Mengge Zhang, Xiuxiu Dong, Guanya Ji, Jing Wang, Tao Wang, Qian Liu, Qijian Niu","doi":"10.1016/j.bios.2024.116870","DOIUrl":"10.1016/j.bios.2024.116870","url":null,"abstract":"<p><p>The development of high photoactive cathode materials combined with the formation of a stable interface are considered important factors for the selective and sensitive photoelectrochemical (PEC) detection of tetracycline (TC). Along these lines, in this work, a novel type II heterostructure composed of two-dimensional (2D) covalent organic frameworks confined to zero-dimensional (0D) carbon quantum dots (CDs/COFs) film was successfully synthesized using the rapid in-situ polymerization method at room temperature. The PEC signal of CDs/COFs was significantly amplified by improving the light absorption and electron transfer capabilities. Furthermore, a cathodic molecularly imprinted PEC sensor (MIP-PEC) for the detection of TC was constructed through fast in-situ Ultraviolet (UV) photopolymerization on the electrode. Finally, a \"turn-off\" PEC cathodic signal was achieved based on the selective recognition of the imprinted cavity and the mechanism of steric hindrance increase. Under optimal conditions, the proposed sensor demonstrated a wide linear relationship with TC in the concentration range of 5.00 × 10^-12-1.00 × 10^-5 M, with a detection limit as low as 6.00 × 10^-13 M. Meanwhile, excellent stability, selectivity, reproducibility, and applicability in real river samples was recorded. Our work provides an effective and rapid in situ construction method for fabricating highly photoactive cathode heterojunctions and uniform stable selective MIP-PEC sensing interfaces, yielding accurate antibiotics detection in the environment.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic engineering of heterostructure and oxygen vacancy in cobalt hydroxide/aluminum oxyhydroxide as bifunctional electrocatalysts for urea-assisted hydrogen production.","authors":"Minglei Yan, Junjie Zhang, Cong Wang, Lang Gao, Wengang Liu, Jiahao Zhang, Chunquan Liu, Zhiwei Lu, Lijun Yang, Chenglu Jiang, Yang Zhao","doi":"10.1016/j.jcis.2024.07.239","DOIUrl":"10.1016/j.jcis.2024.07.239","url":null,"abstract":"<p><p>Designing inexpensive, high-efficiency and durable bifunctional catalysts for urea oxidation reaction (UOR) and hydrogen evolution reaction (HER) is an encouraging tactic to produce hydrogen with reduced energy expenditure. Herein, oxygen vacancy-rich cobalt hydroxide/aluminum oxyhydroxide heterostructure on nickel foam (denoted as Co(OH)₂/AlOOH/NF-100) has been fabricated using one step hydrothermal process. Theoretical calculation and experimental results indicate the electrons transfer from Co(OH)₂ to highly active AlOOH results in the interfacial charge redistribution and optimization of electronic structure. Abundant oxygen vacancies in the heterostructure could improve the conductivity and simultaneously serve as the active sites for catalytic reaction. Consequently, the optimal Co(OH)₂/AlOOH/NF-100 demonstrates excellent electrocatalytic performance for HER (62.9 mV@10 mA cm^-2) and UOR (1.36 V@10 mA cm^-2) due to the synergy between heterointerface and oxygen vacancies. Additionally, the in situ electrochemical impedance spectrum (EIS) for UOR suggests that the heterostructured catalyst exhibits rapid reaction kinetics, mass transfer and current response. Importantly, the urea-assisted electrolysis composed of the Co(OH)₂/AlOOH/NF-100 manifests a low cell voltage (1.48 V @ 10 mA cm^-2) in 1 M KOH containing 0.5 M urea. This work presents a promising avenue to the development of HER/UOR bifunctional electrocatalysts.</p>","PeriodicalId":351,"journal":{"name":"Journal of Colloid and Interface Science","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141974680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boronic acid ester-based hydrogel as surface-enhanced Raman scattering substrates for separation, enrichment, hydrolysis and detection of hydrogen peroxide residue in dairy product all-in-one.","authors":"Ziwang Liu, Rihui Su, Xiaohua Xiao, Gongke Li","doi":"10.1016/j.talanta.2024.126900","DOIUrl":"10.1016/j.talanta.2024.126900","url":null,"abstract":"<p><p>Rapid and selective separation, enrichment and detection of trace residue all-in-one in complex samples is a major challenge. Hydrogels with molecular sieve properties can selectively separate and enrich target analytes, and the combination with high sensitivity detection of surface-enhanced Raman scattering (SERS) is expected to achieve the above all-in-one detection. Herein, the core-shell structured Au@poly(N-isopropylacrylamide)-phenylboronic acid hydrogel (Au@PNIP-VBA) with boronic acid ester groups was prepared by thermally initiated polymerization. The boronic acid ester groups in hydrogel are selectively hydrolyzed by hydrogen peroxide (H₂O₂) to hydroxyl structures, leading to a reduction in SERS signals. The Au@PNIP-VBA hydrogel has molecular sieve properties and high SERS activity, making it suitable for separation, enrichment, hydrolysis and detection of H₂O₂ all-in-one. A rapid SERS method was developed for analysis of H₂O₂ based on the Au@PNIP-VBA hydrogel with the linear range of 8.5 × 10^-2-6.8 mg L^-1 and the detection limit of 33 μg L^-1. The method was successfully applied to the determination of H₂O₂ residue in fresh milk, pure milk, yogurt and camel milk, with the recoveries were in the range of 82.2%-109.3% and the relative standard deviations were 2.8%-8.3%. This efficient all-in-one strategy has the advantages of simple sample pre-treatment, rapid analysis (30 min) and high sensitivity, making it highly promising for food quality and safety analysis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly selective ethanol vapour sensing materials for a new generation of gas sensors based on molecularly imprinted polymers.","authors":"Todd Cowen, Sotirios Grammatikos, Michael Cheffena","doi":"10.1016/j.talanta.2024.126902","DOIUrl":"10.1016/j.talanta.2024.126902","url":null,"abstract":"<p><p>A simple gas sensor consisting of a molecularly imprinted polymer-carbon nanotube composite cast onto a screen-printed electrode has been developed with extremely high selectivity for ethanol vapour over methanol vapour. Ethanol gas sensors typically display selectivity for ethanol over methanol in the range 2-4 times, while the mean ratio of ethanol to methanol response observed with the described device was 672. This selectivity was achieved under ambient conditions. Additionally, the molecularly imprinted polymer was produced using reagents previously applied in the development of a device selective for methanol, with only the template being changed. This demonstrates the versatility of molecular imprinting and provides a foundation for their greater integration into future gas sensors.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paper-based multicolor sensor for on-site quantitative detection of organophosphate pesticides based on acetylcholinesterase-mediated paper-based Au³⁺-etching of gold nanobipyramids and CIELab color space.","authors":"Feng Zhang, Yu Gao, Enxi Ren, Ling Fang, Weijuan Yang, Liaoyuan Zhang, Zongwen Wang","doi":"10.1016/j.talanta.2024.126925","DOIUrl":"10.1016/j.talanta.2024.126925","url":null,"abstract":"<p><p>On-site quantitative detection of organophosphorus pesticides (OPs) is crucial for safeguarding food and public safety. This study presents a novel acetylcholinesterase (AChE)-mediated paper-based Au³⁺-etching of gold nanobipyramids (AuNBPs) system. The system employs a long-term storable AuNBPs-deposited nylon membrane embedded within a portable and temperature-controlled paper-based analytical device. This system, coupled with a colorimeter-based quantitative method, enables the development of a practical paper-based multicolor sensor (PMS) for on-site quantitative detection of three common OPs (paraoxon, dichlorvos, and trichlorfon). In the absence of OPs, AChE hydrolyzes acetylthiocholine to thiocholine, which reduces Au³⁺ to Au⁺. The presence of OPs inhibits AChE activity, thereby preserving Au³⁺ to etch AuNBPs on nylon membranes, accompanied by multicolor changes. These color changes can be simply quantified by measuring the a∗ parameter of the CIELab color space using a portable colorimeter. Under optimal conditions, the PMS displayed eight OPs-corresponding color changes with a minimum detectable concentration of 1.0-10 μg/L (visual observation) and limits of detection of 0.8-7.2 μg/L (colorimeter) and 0.2-3.4 μg/L (UV-vis spectrometry). The PMS successfully determined the OPs in vegetable and rice samples with recoveries of 89.0-109 % and RSDs (n = 5) of <6 %. These results were consistent with those obtained using the HPLC-MS method. The PMS demonstrates excellent portability, AuNBPs stability, detection sensitivity, and reproducibility, making it a promising tool for the on-site quantitative detection of OPs residues in food. Furthermore, the paper-based etching system coupled with the colorimeter-based quantitative method provides a valuable reference to develop practical PMSs for various targets in diverse fields.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of hapten and monoclonal antibody of hesperetin and establishment of enzyme-linked immunosorbent assay.","authors":"Yifan Liu, Zihui Jin, Di Sun, Bo Xu, Tianyu Lan, Qiyang Zhao, Yue He, Jing Li, Yongliang Cui, Yaohai Zhang","doi":"10.1016/j.talanta.2024.126912","DOIUrl":"10.1016/j.talanta.2024.126912","url":null,"abstract":"<p><p>Hesperetin is the aglycone of hesperidin and is widely found in the Rutaceae plants and herbal medicines. It exhibits antioxidant, detoxifying, anti-inflammatory, and antimicrobial properties, similar to hesperidin. It has also shown potential in the regulation of certain diseases. In order to detect hesperetin in complex matrix samples such as citrus and herbal, we developed an anti-hesperetin monoclonal antibody and established an indirect competitive enzyme-linked immunosorbent assay (icELISA). The half maximal inhibitory concentration (IC₅₀) was determined to be 2.03 ng/mL, the detection range was 0.39-12.73 ng/mL. In practical sample testing, the concentration of hesperidin measured by icELISA is consistent with the result of UPLC-MS/MS, and the correlation coefficient (R²) is 0.97. These results showed that the established method has good accuracy, reproducibility and broad application prospects, and can be used for the detection of hesperetin in complex matrix samples (such as citrus and herbal samples).</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}