Elsevier最新文献

{"title":"Study of cell migration trajectory on two-dimensional continuous stiffness gradient surface edited by grayscale photopolymerization.","authors":"Kin Fong Lei, Kuo-Cheng Bai, Ping-Ching Pai","doi":"10.1016/j.talanta.2024.126899","DOIUrl":"10.1016/j.talanta.2024.126899","url":null,"abstract":"<p><p>In native tissues, cells encounter a diverse range of stiffness, which can significantly affect their behavior and function. The ability of cells to sense and respond to these mechanical cues is essential for various physiological processes, including cell migration. Cell migration is a complex process influenced by multiple factors, with substrate stiffness emerging as a critical determinant. This study developed a technique to edit the stiffness of polyacrylamide (PAA) hydrogel substrates by adjusting the grayscale level of a photomask during photopolymerization. By analyzing cell morphologies on the hydrogel, we confirmed the development of a single PAA hydrogel substrate with continuous stiffness gradients. This method was used to explore the correlation between substrate stiffness and cell migration dynamics. The study found that cells typically migrated from softer to stiffer surfaces. When the cells initially located on stiffer surfaces, they were able to travel longer distances. Additionally, a continuous 2D stiffness gradient surface was fabricated to explore how cells migrate on smoother versus steeper stiffness gradients. The results showed that cells tended to migrate more readily on smoother stiffness gradient surfaces compared to steeper ones. This study provides valuable insights into cell migration dynamics on substrates with varying stiffness gradients. The results underscore the importance of the mechanical environment in cancer cell migration and offer promising directions for developing interventions to prevent cancer spread.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Target-promoted activation of DNAzyme walker for in situ assembly of hemin/G-quadruplex nanowires enable ultrasensitive and label-free electrochemical myocardial microRNA assay.","authors":"Tengteng Shan, Lingrong Cui, Huimin Zhang, Kaiqin Li, Jianmei Yang, Yan Zhao, Yun Xiang, Ruo Yuan","doi":"10.1016/j.talanta.2024.126923","DOIUrl":"10.1016/j.talanta.2024.126923","url":null,"abstract":"<p><p>The concentration elevation of myocardial microRNA (miRNA) biomarker is associated with the pathogenic process of acute myocardial infarction (AMI), and sensitive quantification of myocardial miRNA biomarker plays an important role for early AMI diagnosis and its treatment. In response, this work describes an ultrasensitive and non-label electrochemical biosensor for the assay of myocardial miRNA based on cascade signal amplifications integrated by DNAzyme walker and hemin/G-quadruplex nanowires. The DNAzyme walker is activated by presence of target miRNAs to move along the electrode surface to cyclically cleave the substrate hairpins to release G-quadruplex segments, which further trigger the in situ formation of many hemin/G-quadruplex nanowires. The large amounts of hemin intercalated into the DNA nanowires subsequently generate drastically magnified electrochemical current signals for highly sensitive label-free assay of myocardial miRNAs down to 15.7 fM within dynamic range of 100 fM to 10 nM. Such a biosensor also has high selectivity and can monitor myocardial miRNAs in diluted serums at low levels, providing a sensitive and reliable platform for diagnosing infarct-associated cardiovascular diseases.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142306870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amino-rich silicon quantum dots as efficient activator with intrinsic chemiluminescence for the detection of peroxydisulfate.","authors":"Dayang Zhao, Hui Gong, Houjing Liu","doi":"10.1016/j.talanta.2024.126931","DOIUrl":"10.1016/j.talanta.2024.126931","url":null,"abstract":"<p><p>The specific detection of peroxydisulfate (S₂O₈^2-, PDS) is significant and challenging due to the rapid development of PDS-related technologies and their widespread application in multiple fields. However, traditional analytical methods are mainly based on their strong oxidizing properties, making it difficult to simultaneously achieve specific identification and high sensitivity for PDS detection in complex water environments. Here, we purposely prepared amino-rich SiQDs (N-SiQDs) as an effective catalyst and introduced H₂O₂ acts as a co-reactant for PDS activation and determination with strong intrinsic chemiluminescence (CL) emission. High yield of reactive active oxygen (mainly O₂˙^- and ˙OH) were generated during CL process, which trigger electron-hole annihilation between the N-SiQDs˙⁺ and N-SiQDs˙^- accounted for extraordinary CL emission. On this basis, a new CL assay for PDS detection was fabricated with broad linear range of 5 × 10^-7M-5 × 10^-5 M and low detection limit (3.2 × 10^-7 M). Due to the absence of SO₄˙^- involvement during CL emission, the sensing platform is sensitive enough, satisfactory selectivity and does not respond to transition-metal ions and inorganic anions that have interferences in the PDS CL sensors reported before. This work not only deepens insight into the mechanisms of nanomaterials assisted PDS activation but also provides a new perspective on the modified metal-free QDs CL probe for chemical species detection.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Probiotic bacterial adsorption coupled with CRISPR/Cas12a system for mercury (II) ions detection\" [Biosens. Bioelectron. 263 (2024) 116627].","authors":"Ying Yu, Yuan Zhang, Yining Zhao, Kangzheng Lv, Lianzhong Ai, Zhengjun Wu, Zibo Song, Juan Zhang","doi":"10.1016/j.bios.2024.116831","DOIUrl":"10.1016/j.bios.2024.116831","url":null,"abstract":"","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The value of electrochemical ratiometry in immunosensing: A systematic study.","authors":"Jin Song, Rui Gong, Shibo Song, Ghulam Abbas, Yaohong Ma, Yiwei Li","doi":"10.1016/j.bios.2024.116817","DOIUrl":"10.1016/j.bios.2024.116817","url":null,"abstract":"<p><p>Reluctant reproducibility and accuracy make electrochemical immunosensors suffering from high possibility of false negative/positive results, and it is the main obstacle that hinders them into an eligible alternative technology to the gold-standard method. It has been demonstrated sporadically previously that ratiometry helps deal with this issue but to what extent this could be beneficial and why it could fulfill is yet to be explored. In this study, to the best of our knowledge, for the first time, we have attempted to answer these questions through comprehensive experiments. For this purpose, labeled and label-free electrochemical immunosensors for SARS-CoV-2 pseudovirus quantification are constructed as a model electrochemical immunosensor. Conventional and ratiometric immunosensors are prepared by using electrochemically synthesized graphene modified electrodes coupled with various electrochemical probe pairs. It was found that the electrocatalyst modification at the electrode interface makes the predominant contribution to immunosensor sensitivity, while appropriate ratiometry provided electrochemical immunosensors with significantly enhanced reproducibility, accuracy, as well as sensing stability. Further, the experiments confirmed that the improvement in sensor performance achieved by ratiometry is primarily through overcoming the inherent errors and dynamic variations of the base electrode. It is also demonstrated electrochemical immunosensors made thereof could easily rival the performances of the gold-standard PCR method, in the view of immunoassay diagnosis. Therefore, it is of great promise to evolve electrochemical immunosensors into an eligible substitute technique towards the prevalent nucleic acid detection method in point-of-care testing (POCT), with the aid of electrochemical ratiometry.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Excellent properties of NaF and NaBr induced DNA/gold nanoparticle conjugation system: Better stability, shorter modified time, and higher loading capacity.","authors":"Hai-Bo Wang, Liang Zhang, Tian-Yu Hu, Xue-Qing Yuan, Sheng-Wei Huang, Jin-Quan Li, Zi-Tao Zhong, Yuan-Di Zhao","doi":"10.1016/j.bios.2024.116876","DOIUrl":"10.1016/j.bios.2024.116876","url":null,"abstract":"<p><p>The functionalization of gold nanoparticle (AuNP) is the key procedure for the biochemical and biomedical application. The conventional salt-aging method requires the stepwise additions of NaCl and excessive thiolated DNA, mainly due to the poor tolerance of the DNA/AuNP mixture toward NaCl. Herein, we found that NaF is capable of improving the stability for the modification of AuNP with different bases of DNA sequences (poly A/T/C/G), and allows for adding up with a high concentration of 200 mM at one time, which greatly reduces the total modification time to 0.5-1 h. Intriguingly, the introduction of NaBr effectively increases the DNA loading capacity. Besides the advantages of NaF and NaBr, the modification performance is improved via the introduction of the oligo A/T spacer for the G-rich DNA sequences. Furthermore, this method shows the superiority to another two methods (pH 3-based and salt-aging) in terms of the loading capacity or sequence components.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing oxygen vacancy-rich CuMn₂O₄/carbon dots dual-function nanozymes via Chan-Lam coupling reaction for the colorimetric/fluorescent determination of D-penicillamine.","authors":"Min Feng, Xiaodan Zhang, Yuming Huang","doi":"10.1016/j.bios.2024.116864","DOIUrl":"10.1016/j.bios.2024.116864","url":null,"abstract":"<p><p>Defect engineering is a promising approach to construct high performance nanozymes due to its ability to regulate their physical and chemical properties. However, how to construct defects to improve the activity of nanozymes remains a challenge. Herein, for the first time, the Chan-Lam coupling reaction is used to construct the oxygen vacancy (O_V)-rich CuMn₂O₄/carbon dots (CDs) (O_V-CuMn₂O₄/CDs) dual-function nanozymes with fluorescent (FL) and oxidase-like properties, via regulating the low-valent metal ions (Cu⁺ and Mn²⁺) and O_v contents in the spinel CuMn₂O₄ and in-situ growth of β-cyclodextrin (β-CD)-derived CDs. Expectedly, relative to CuMn₂O₄, the O_V-CuMn₂O₄/CDs exhibited 35.8%, 8.5%, and 14.6% rise in the contents of Cu⁺, Mn²⁺ and O_v, respectively. Abundant O_v provides more O₂ adsorption/activation sites, and the charge transfer between O_v and metal atoms increases the charge density around metal atoms. This produces more low-valent metals (like Cu⁺ and Mn²⁺) to promote the electron transfer from metal to O atoms and O-O bond cleavage. Thus, the oxidase-like activity of O_V-CuMn₂O₄/CDs is 4.1 times that of CuMn₂O₄. Also, the in-situ growth of β-CD-derived carbon dots on CuMn₂O₄ endows O_V-CuMn₂O₄/CDs selective target recognition. Thus, a sensitive and selective colorimetric and fluorescence dual-mode method was established for determining D-penicillamine (D-PA), with the limit of detection of 0.25 and 0.048 μM, respectively. The method was applied to D-PA determination in real samples. This work demonstrates the Chan-Lam coupling reaction can be used to construct high performance nanozymes for developing dual-mode sensor for the detection of targets.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice to \"A comprehensive study on transparent conducting oxides in compact microbial fuel cells: Integrated spectroscopic and electrochemical analyses for monitoring biofilm growth\" [Biosens. Bioelectron. 250 (2024) 116067].","authors":"Raden Priyo Hartono Adji, Isa Anshori, Robeth Viktoria Manurung, Taufiqqurrachman, D Mahmudin, Pamungkas Daud, Deni Permana Kurniadi, Eko Joni Pristianto, Arief Nur Rahman, Winy Desvasari, Sulistyaningsih, Raden Deasy Mandasari, Hiskia, Goib Wiranto","doi":"10.1016/j.bios.2024.116812","DOIUrl":"10.1016/j.bios.2024.116812","url":null,"abstract":"","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conductive single enzyme nanocomposites prepared by in-situ growth of nanoscale polyaniline for high performance enzymatic bioelectrode.","authors":"Han Sol Kim, Kyungmin Ahn, Byeol Yi Han, Al-Monsur Jiaul Haque, Sujin Kim, Seungkeun Kim, Youngho Wee, Jungbae Kim","doi":"10.1016/j.bios.2024.116841","DOIUrl":"10.1016/j.bios.2024.116841","url":null,"abstract":"<p><p>Enzyme-based electrochemical biosensors hold great promise for applications in health/disease monitoring, drug discovery, and environmental monitoring. However, inherently non-conductive nature of proteinaceous enzymes often hampers effective electron transfer at enzyme-electrode interface, limiting biosensor performance of enzyme bioelectrodes. To address this problem, we present an approach to synthesize polyaniline (PAN)-based conductive single enzyme nanocomposites of glucose oxidase (GOx) (denoted as PAN-GOx). To prevent multimerization of enzymes during nanocomposite synthesis and enable single enzyme wrapping, we activate GOx surface with phenylamine groups based on the programmed diffusion of reactants in the reaction solution. Subsequent in-situ polymerization enables the synthesis of nanoscale conductive PAN layer (∼2.7 nm thickness) grafted from individual GOx molecule. PAN-GOx retains 83% and 74% of its specific activity and catalytic efficiency, respectively, compared to free GOx, while demonstrating a ∼500% improved conductivity. Furthermore, PAN-GOx-based glucose biosensors show an approximately 16- and 3-fold higher sensitivity compared to biosensors prepared by using free GOx and a mixture of PAN and GOx, respectively. This study provides a facile method to fabricate conductive single enzyme nanocomposites with enhanced electron transfer, which can potentially be further modified and/or compounded with conductive materials for demonstrating high performance enzymatic bioelectrodes.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatible sensors for ammonia gas detection.","authors":"Maria Angustias Torres-Molina, Miguel M Erenas, Mariano Ortega Munoz, Luis Fermin Capitan Vallvey, Isabel M Perez de Vargas Sansalvador","doi":"10.1016/j.talanta.2024.126916","DOIUrl":"10.1016/j.talanta.2024.126916","url":null,"abstract":"<p><p>In this work, three different dyes have been tested for the determination of gaseous ammonia. This gas is one of the products of microbial degradation and therefore its presence is an indicator of deterioration and could be used as a food freshness indicator. Three different sensors have been prepared and tested, two of them using the natural pigments curcumin and anthocyanin and the other one using bromothymol blue. All of them are biocompatible and therefore allowed to use in contact with food. Different compositions, materials for deposition, stability and reversibility for ammonia gas detection have been studied under high humidity conditions simulating real packaged food conditions. Colorimetry is the technique used to obtain the analytical parameter, the H coordinate of the HSV colour space, simply using a camera, avoiding the use of complex instrumentation. Sensibility, toxicity grade and stability found show that the sensor could be implemented in packaged food and form the basis of a freshness indicator for the food industry.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}