Cellular Physiology and Biochemistry最新文献

{"title":"MOTS-c Impact on Muscle Cell Differentiation and Metabolism Across Fiber Types.","authors":"Natalia Leciejewska, Ewa Pruszyńska-Oszmałek, Paweł Kołodziejski, Dawid Szczepankiewicz, Leszek Nogowski, Maciej Sassek","doi":"10.33594/000000755","DOIUrl":"https://doi.org/10.33594/000000755","url":null,"abstract":"<p><strong>Background/aims: </strong>MOTS-c belongs to a group of mitochondrial peptides involved in metabolic processes in the body. This peptide has garnered increasing attention since its discovery in 2015 because of its potential to ameliorate metabolic parameters in animals with diabetes or insulin resistance. MOTS-c is involved in muscle metabolism; however, little is known about its role in fiber differentiation.</p><p><strong>Methods: </strong>We conducted a study to explore the effect of MOTS-c on cellular processes using the C2C12 and L6 cell lines, representing different metabolic types of muscle fibers. The research methods were real-time PCR, Western blot, and lipid accumulation measurement.</p><p><strong>Results: </strong>Notably, our investigations revealed that MOTS-c increased the survival of C2C12 cells at doses of 10 and 100 nM (p<0.01) and stimulated the phosphorylation of extracellular signal-regulated kinase within 5 min of incubation (p<0.05). Remarkably, these effects were not observed in L6 cells; however, both cell lines showed a reduced rate of proliferation. Furthermore, MOTS-c promotes the differentiation of C2C12 cells by increasing the expression of muscle regulatory factors, but it does not produce such an effect in L6 cells. Additionally, cells were treated with physiological concentrations of free fatty acids and MOTS-c, unveiling an augmentation in lipid accumulation observed in L6 cells and a decrease in lipid accumulation in C2C12 cells.</p><p><strong>Conclusion: </strong>In conclusion, our findings have suggested a diverse response to MOTS-c depending on the type of muscle fibers, particularly in the domains of survival, cell differentiation, and lipid accumulation.</p>","PeriodicalId":9845,"journal":{"name":"Cellular Physiology and Biochemistry","volume":"59 1","pages":"34-46"},"PeriodicalIF":2.5,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Connection Between Ion Channels and Pancreatic Stellate Cell Activation.","authors":"Julie Auwercx, Mathilde Fourgeaud, Alexis Lalot, Mathieu Gautier","doi":"10.33594/000000754","DOIUrl":"https://doi.org/10.33594/000000754","url":null,"abstract":"<p><p>Quiescent pancreatic stellate cells (PSCs) represent only a very low proportion of the pancreatic tissue, but their activation leads to stroma remodeling and fibrosis associated with pathologies such as chronic pancreatitis and pancreatic ductal adenocarcinoma (PDAC). PSC activation can be induced by various stresses, including acidosis, growth factors (PDGF, TGFβ), hypoxia, high pressure, or intercellular communication with pancreatic cancer cells. Activated PSC targeting represents a promising therapeutic strategy, but little is known regarding the molecular mechanisms underlying the activation of PSCs. Identification of new biomarkers of PSC activation associated with desmoplasia in chronic pancreatitis and PDAC could lead to new therapeutic targets for exocrine pancreatic disease treatments. Ion channels and transporters are transmembrane proteins involved in numerous physiological and pathological processes, including PDAC. They are well known to act as biosensors of the tissue microenvironment, and they can be easily accessible for drugs. However, their role in PSC activation is not fully understood. In this review, we briefly discuss the role of activated PSCs in pancreas inflammation and pathological fibrosis (associated with chronic pancreatitis and PDAC), and we describe the role of specific ion channels and transporters (Ca²⁺, K⁺, Na⁺ and Cl^-) in these processes in the light of recent literature.</p>","PeriodicalId":9845,"journal":{"name":"Cellular Physiology and Biochemistry","volume":"59 S1","pages":"25-40"},"PeriodicalIF":2.5,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Ph on the Physicochemical Properties of a Cassava Peel Starch Biopolymer.","authors":"Lisbeth Anchundia, Felipe Jadán Piedra, José Alejandro Macías Alcívar, Virginia Sánchez Mendoza, Danny Isaías Vera Guerrero, Sonia Nathaly Giler Intriago, Wagner Antonio Gorozabel Muñoz, Grether Lucía Real Pérez, Ricardo Loor Alava, Odalis Barzallo Delgado, Carlos Jadán Piedra","doi":"10.33594/000000753","DOIUrl":"https://doi.org/10.33594/000000753","url":null,"abstract":"<p><strong>Background/aims: </strong>This study investigates how pH levels affect the characteristics of biopolymer films manufactured from cassava peel starch. Cassava peel starch's abundance and biodegradability make it a promising candidate for sustainable packaging. The study seeks to improve film qualities such as thickness, density, moisture content, solubility, and optical properties by altering pH levels. Understanding these effects is critical for increasing the acceptability of cassava peel starch biopolymers in a variety of industrial applications, notably environmentally friendly packaging solutions.</p><p><strong>Methods: </strong>Starch extracted from cassava peel was used to produce films using the casting method at specified pH levels. The films were evaluated for thickness and density using classical methods. Moisture content was determined following the AOAC 930.15 (2000) protocol. Color analysis was conducted using the CIELab color space technique. Water solubility and solubility in acidic (HCl) and alkaline (NaOH) solutions were assessed through chemical solubility tests performed by gravimetry.</p><p><strong>Results: </strong>The study investigated how pH impacts biopolymer films manufactured from cassava peel starch. The film thickness varied greatly across pH levels, with pH 10.5 creating the thickest films (0.158 ± 0.012 mm) and pH 6.5 providing the thinnest (0.118 ± 0.015 mm). Density varied slightly, from 1.393 ± 0.122 g/cc to 1.551 ± 0.153 g/cc. Moisture content fluctuated significantly, affecting biodegradability. Color study indicated pH-dependent variations in transparency and opacity, with higher pH values resulting in larger color deviations (∆E). Water solubility remained constant, but NaOH solubility dropped with increasing pH, peaking at pH 7.5 (23.44 ± 2.82%).</p><p><strong>Conclusion: </strong>This work investigates the use of cassava peel starch for biopolymer synthesis at controlled pH levels. The findings demonstrate the material's practicality and provide critical insights for enhancing film qualities, particularly in a variety of industrial applications and environmentally friendly packaging solutions.</p>","PeriodicalId":9845,"journal":{"name":"Cellular Physiology and Biochemistry","volume":"59 1","pages":"21-33"},"PeriodicalIF":2.5,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone Marrow Mesenchymal Stem Cells Promote Repairing the Bruised Tissue via Regulating mRNA Expression of Molecular Biomarkers and the Apoptotic Rate.","authors":"Doaa Ramadan I Abdel-Gawad, Walaa A Moselhy, Rasha Rashad Ahmed, Hessah M Al-Muzafar, Kamal Adel Amin, Khaled Abbas Helmy Abdou","doi":"10.33594/000000752","DOIUrl":"https://doi.org/10.33594/000000752","url":null,"abstract":"<p><strong>Background/aims: </strong>Bruise is the extravasation of blood that may be mild or severe. Bone marrow mesenchymal stem cells (BM-MSCs) are one of the most promising cells used in regenerative medicine for treating many disorders. We aimed to evaluate the efficiency of BM-MSCs in treating cutaneous bruises.</p><p><strong>Methods: </strong>78 male albino rats were equally divided into 3 groups, control group (G1), bruise wound group (G2) and Bruised animals treated with BM-MSCs group (G3). The sequences of color changes were recorded. Animals were sacrificed and skin samples were collected for histopathological examination and analyzing the mRNA expression rate of transforming growth factor- β (TGF-β), interleukin-6 (IL-6), tumor necrotic factor-α (TNF-α), Heat shock protein-90 α (HSP-90α), Metalloprotiens-9 (MMP-9), and microRNA-21 (miR-21), which incorporated in the healing process and the apoptotic rat.</p><p><strong>Results: </strong>Subcutaneous injection of BM-MSCs reduced the color intensity of the bruised skin, with statistically significant upregulation of TGF-β, TNF-α, and HSP-90α, significant down-regulation of MMP-9 and miR-21 mRNA expression rate, and significant reduction of the apoptotic rate and the inflammatory cells.</p><p><strong>Conclusion: </strong>BM-MSCs have a promising improvement in the healing process of bruises by regulating the expression rate of TGF-β- IL-6- TNF-α- HSP-90α- MMP-9- miR-21 and reducing the apoptotic rate and inflammatory cell infiltration.</p>","PeriodicalId":9845,"journal":{"name":"Cellular Physiology and Biochemistry","volume":"59 1","pages":"1-20"},"PeriodicalIF":2.5,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammatory Pathways of Sulfonamide Diuretics: Insights into SLC12A Cl^- Symporters and Additional Targets.","authors":"Mauricio Di Fulvio","doi":"10.33594/000000751","DOIUrl":"https://doi.org/10.33594/000000751","url":null,"abstract":"<p><p>Thiazide, thiazide-like, and loop diuretics are primarily known for inhibiting members of the SLC12A family of Cl^- transporters, which include the Na+Cl^- cotransporter (NCC), Na⁺K⁺2Cl^- cotransporters (NKCC1 and NKCC2) and K⁺Cl^- symporters (KCC1-4). While the main pharmacological effect of these diuretics is diuresis, achieved by promoting the excretion of excess water and salt through the kidneys, they have intriguing pharmacological effects beyond their traditional ones which cannot be solely attributed to their effects on renal salt transport. Of particular interest is their role in modulating inflammatory processes. These diuretics appear to exert both pro- and anti-inflammatory effects, potentially by influencing various pathways involved in immune responses. For example, NKCC1 has been implicated in the regulation of pro-inflammatory cytokines, such as interleukin-1β (IL1β), interleukin-8 (IL8) and tumor necrosis factor α (TNFα), which are critical mediators of immune cell activity during inflammation. The underlying mechanisms through which NKCC1 contributes to inflammation may involve key signaling pathways, such as that mediated by the nuclear factor kappa B (NFκB). This pathway is crucial for the activation and assembly of the inflammasome, as well as for regulating the phagocytic activity of immune cells. In addition, NKCC1 can control (or be controlled) by reactive oxygen species and oxidative stress, which contribute to the pathogenesis of various inflammatory conditions as well. Diuretics may help mitigate inflammation-related tissue damage by scavenging reactive oxygen species and boosting antioxidant defenses, thereby restoring redox balance in inflamed tissues. Despite these intriguing effects, the precise molecular pathways through which thiazide, thiazide-like and loop diuretics may modulate inflammatory responses remain poorly understood and warrant further investigation. This aspect of their pharmacological profile highlights their potential for therapeutic use beyond the scope of traditional diuretic functions.</p>","PeriodicalId":9845,"journal":{"name":"Cellular Physiology and Biochemistry","volume":"59 S1","pages":"1-24"},"PeriodicalIF":2.5,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration the Effects of Mesenchymal Stem Cells and Olive Leaf Extract on Physiological and Histopathological Changes on the Kidney of Diabetes Rats.","authors":"Mervat Ahmed AbdRabou, Ahmed Atwa, Zahrah R Alrayes, Diaa Massoud, Fawzyah A Alghamdi, Aml M Asran, Mousa O Germoush, Hanan M Alharbi, Hadeel K Alruwaili, Khadija Abdul Jalil Faddladdeen, Ahmed B M Mehany","doi":"10.33594/000000748","DOIUrl":"https://doi.org/10.33594/000000748","url":null,"abstract":"<p><strong>Background/aims: </strong>Gestational Diabetes Mellitus (GDM) is a common complication during pregnancy, defined as diabetes diagnosed in the second or third trimester, often asymptomatic. This study investigates the therapeutic potential of olive leaf extracts and stem cells in mitigating GDM-induced complications, particularly focusing on renal function, oxidative stress, and pancreatic cell regeneration.</p><p><strong>Methods: </strong>Measurements were made in gravid female rats with or without intraperitoneal administration of Streptozotocin (35 mg/kg body weight). Biochemical analyses were conducted to evaluate renal function markers (urea, uric acid, creatinine) and oxidative stress parameters (malondialdehyde, glutathione, and superoxide dismutase levels). Histopathological and immunohistopathological evaluations of kidney tissues were performed using hematoxylin and eosin staining and specific markers (p53, Insulin, and PCNA) to assess cellular changes.</p><p><strong>Results: </strong>The diabetic group exhibited significantly elevated levels of urea, uric acid, and creatinine (p<0.01) compared to the control group. Treatment with stem cells and olive leaf extracts significantly reduced these levels. Malondialdehyde levels were elevated in the diabetic group (p<0.01) but showed marked improvement in the treatment groups. Additionally, glutathione and superoxide dismutase activities were diminished in the diabetic rats (p<0.05) but increased following treatment. Histopathological and immunohistopathological analyses revealed cellular regeneration and improved tissue morphology in the treatment groups compared to the diabetic group.</p><p><strong>Conclusion: </strong>Stem cells and olive leaf extracts exhibit significant therapeutic potential in ameliorating renal dysfunction, oxidative stress, and tissue damage associated with GDM, highlighting their role in enhancing pancreatic cell regeneration.</p>","PeriodicalId":9845,"journal":{"name":"Cellular Physiology and Biochemistry","volume":"58 6","pages":"807-822"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimisation of Blood Donor Nutrition: Blood Donor Health Improvement Studies.","authors":"Natalia Kurhaluk, Małgorzata Gradziuk, Halina Tkaczenko","doi":"10.33594/000000747","DOIUrl":"https://doi.org/10.33594/000000747","url":null,"abstract":"<p><p>This review provides an analysis of the current literature on the health and nutrition of blood donors, examining key aspects that affect the quality of donated blood and the well-being of donors. The review discusses effective iron absorption facilitated by key nutrients and presents evidence on the importance of a balanced diet rich in essential nutrients, such as vitamin B12 and folic acid. The review examines the differences in iron levels between men and women and highlights the role of sex hormones in regulating iron metabolism. In addition, the review discusses the link between psycho-emotional well-being and diet, showing that proper dietary habits can improve mental health, reduce stress, and enhance the donation experience. This article provides practical recommendations to support donor well-being and the effectiveness of blood donation programmes worldwide. By highlighting the differences between a modern diet and a diet tailored specifically for blood donors, we aim to emphasise the importance of a nutrient-dense diet for blood donors, which is critical for effective recovery and overall health maintenance. It is important to understand and incorporate different nutrient-dense foods that influence iron absorption for optimal health in blood donors. Donor health is also influenced by regular physical activity and psycho-emotional well-being. The 'healthy donor effect' and its implications for maintaining higher standards of donor health are explored in this review. This article highlights the fact that the diet of blood donors is influenced by gender, as men and women have different nutritional needs and physiological responses, particularly with regard to iron levels and recovery. The importance of enhancing or inhibiting iron absorption provides valuable evidence for food fortification as a cost-effective solution to reduce iron deficiency in blood donors.</p>","PeriodicalId":9845,"journal":{"name":"Cellular Physiology and Biochemistry","volume":"58 6","pages":"756-806"},"PeriodicalIF":2.5,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurate and Efficient Algorithm for Detection of Alzheimer Disability Based on Deep Learning.","authors":"Fayez Alfayez, Sergey Rozov, Mohamed S El Tokhy","doi":"10.33594/000000746","DOIUrl":"https://doi.org/10.33594/000000746","url":null,"abstract":"<p><strong>Background/aims: </strong>Alzheimer's Disease (AD) is a progressive neurodegenerative disorder that severely affects cognitive functions and memory. Early detection is crucial for timely intervention and improved patient outcomes. However, traditional diagnostic tools, such as MRI and PET scans, are costly and less accessible. This study aims to develop an automated, cost-effective digital diagnostic approach using deep learning (DL) and computer-aided detection (CAD) methods for early AD identification and classification.</p><p><strong>Methods: </strong>The proposed framework utilizes pretrained convolutional neural networks (CNNs) for feature extraction, integrated with two classifiers: multi-class support vector machine (MSVM) and artificial neural network (ANN). A dataset categorized into four groups-non-demented, very mild demented, mild demented, and moderate demented-was employed for evaluation. To optimize the classification process, a texture-based algorithm was applied for feature reduction, enhancing computational efficiency and reducing processing time.</p><p><strong>Results: </strong>The system demonstrated high statistical performance, achieving an accuracy of 91%, precision of 95%, and recall of 90%. Among the initial set of twenty-two texture features, seven were identified as particularly effective in differentiating normal cases from mild AD stages, significantly streamlining the classification process. These results validate the robustness and efficacy of the proposed DL-based CAD system.</p><p><strong>Conclusion: </strong>This study presents a reliable and affordable solution for early AD detection and diagnosis. The proposed system outperforms existing state-of-the-art models and offers a valuable tool for timely treatment planning. Future research should explore its application to larger, more diverse datasets and investigate integration with other imaging modalities, such as MRI, to further enhance diagnostic precision.</p>","PeriodicalId":9845,"journal":{"name":"Cellular Physiology and Biochemistry","volume":"58 6","pages":"739-755"},"PeriodicalIF":2.5,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142884979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-Treatment with Cranberry and Vitamin-C Mitigates Reproductive Toxicities Induced by Phenobarbital in Male Rats.","authors":"Heba Ali Abd El-Rahman, Ahmed Flayyih Hasan, Thura Alyasiri, Hany M El-Wahsh, Sarah A Althubyani, Mohamed A Basyony, Sabry Ali El-Naggar, Doha Hanafy Mahmod","doi":"10.33594/000000745","DOIUrl":"https://doi.org/10.33594/000000745","url":null,"abstract":"<p><strong>Background/aims: </strong>Phenobarbital (PB), commonly used for epilepsy management, is associated with testicular dysfunction after prolonged use. This study aimed to evaluate the ameliorative effects of cranberry (CB) and vitamin C (Vit-C) on PB-induced reproductive toxicity in rats.</p><p><strong>Methods: </strong>Forty male Wistar rats were divided into five groups. G1 was the negative control, while G2 received PB (160 mg/kg orally) for one month. Groups G3 and G4 received PB followed by CB (500 mg/kg) and Vit-C (27 mg/kg) treatments, respectively. G5 received PB followed by a combined CB and Vit-C regimen. The levels of catalase (CAT), superoxide dismutase (SOD), glutathione reduced (GSH), and malondialdehyde (MDA) were determined using standard biochemical assays. Histological changes in testicular tissues were assessed, and caspase-3 expression was quantified via immunohistochemistry.</p><p><strong>Results: </strong>PB exposure increased MDA levels, reduced SOD and CAT activity, and disrupted testicular histology, with elevated caspase-3 expression indicating heightened apoptosis. Treatment with CB or Vit-C significantly restored antioxidant enzyme activities, reduced MDA levels, and ameliorated histological abnormalities. Co-treatment with CB and Vit-C yielded the most pronounced protective effects, including reduced caspase-3 expression and improved testicular structure.</p><p><strong>Conclusion: </strong>CB and Vit-C demonstrate significant protective effects against PB-induced testicular toxicity, likely due to their antioxidative and anti-apoptotic properties. Co-administration of these agents offers an effective strategy to mitigate reproductive toxicities associated with prolonged PB use.</p>","PeriodicalId":9845,"journal":{"name":"Cellular Physiology and Biochemistry","volume":"58 6","pages":"722-738"},"PeriodicalIF":2.5,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142884980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Effect of Mesenchymal Stem Cells and Olive Leaf Extract Against Cytotoxicity Induced by Streptozotocin in Gestational Diabetes Rats.","authors":"Mervat Ahmed AbdRabou, Ahmed Atwa, Diaa Massoud, Aml M Asran, Zahrah R Alrayes, Aljohara M Al- Otaibi, Fawzyah A Alghamdi, Mousa O Germoush, Hadeel K Alruwaili, Ahmed Nabeeh, Ahmed B M Mehany","doi":"10.33594/000000744","DOIUrl":"https://doi.org/10.33594/000000744","url":null,"abstract":"<p><strong>Background/aims: </strong>Gestational Diabetes Mellitus (GDM), a prevalent complication in pregnancy, is characterized by the Diabetes Association as diabetes diagnosed in the second or third trimester, often remaining asymptomatic. This study investigates the intricate effects of Streptozotocin on pregnant rats, unraveling its impact on Gestational Type 2 Diabetes (GTD). The research delves into the potential therapeutic roles of mesenchymal stem cells (MSCs) and olive leaf extract (OLE) in mitigating the consequences of Streptozotocin-induced pancreatic impairment.</p><p><strong>Methods: </strong>Female rats were divided into control group, diabetic group, group treated with stem cells after diabetes, group treated with olive leaf extract after diabetes, and group treated with olive leaf extract and stem cells after diabetes. A comprehensive analysis was conducted for all study groups, including diabetic patients' files, hormonal dynamics, histopathological changes, tissue immune responses, and antioxidant mechanisms for all study groups.</p><p><strong>Results: </strong>The results of the study showed that diabetes causes changes in the level of insulin, HB A1C, Fructosamine, HOMA-IR and hormones compared to control group. On the contrary, there was a noticeable improvement in these parameters after treatment with olive leaf extract and stem cells. The results of the present study demonstrated that the combination of OLE and MSCs emerges as a promising approach, demonstrating enhanced glycemic control, modulation of insulin resistance, and improvements in pancreatic islet health. Aso, histopathological results underscore positive effects on pancreatic islet integrity.</p><p><strong>Conclusion: </strong>Stem Cells and Olive Leaf Extract demonstrate favorable effects on regenerative pancreatic cells under normal conditions.</p>","PeriodicalId":9845,"journal":{"name":"Cellular Physiology and Biochemistry","volume":"58 6","pages":"702-721"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}