Association of IL4 (Rs2243250) Gene Variant and Mycoplasma Pneumoniae Infection with Asthma Susceptibility in an Iraqi Population.

IF 2.5 Q3 CELL BIOLOGY

Cellular Physiology and Biochemistry Pub Date : 2025-05-19 DOI:10.33594/000000778

Sarah Kassab Shandaway Al-Zamali, Shahad Saad Mohammed, Safa Hasan Radhi, Sara Aqeel Hassan, Ghufran Abd Omran Abdulridha

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引用次数: 0

Abstract

Background/aims: Asthma is a multifactorial disease influenced by both genetic and environmental factors. This study aimed to investigate the association between the IL4 gene polymorphism (rs2243250) and asthma susceptibility, along with serum IL-4 levels. Additionally, it explored Mycoplasma pneumoniae infection as a potential risk factor for asthma.

Methods: A total of 118 individuals were enrolled, including 60 asthma patients and 58 healthy controls. Genotyping for IL4 rs2243250 was performed using allele-specific PCR (AS-PCR). Previous Mycoplasma pneumoniae infection was assessed serologically, and serum IL-4 levels were measured using ELISA.

Results: No significant differences were observed between groups in terms of age, sex, or residence. Smoking (OR: 7.85, P = 0.001) and family history of asthma (OR: 5.33, P = 0.004) were identified as significant risk factors. Mycoplasma pneumoniae infection was significantly more prevalent in asthma patients (41.7%) than in controls, with a strong association with asthma risk (OR: 8.75, P < 0.0001). Genotype frequencies of rs2243250 differed significantly: CC (36.7% vs. 68.9%), CT (41.7% vs. 24.2%), and TT (21.6% vs. 6.9%) in patients versus controls, respectively. The T allele was more frequent among patients (42.5%) than controls (18.97%), increasing asthma risk (OR: 3.16, P = 0.0001). Both CT (OR: 3.25) and TT (OR: 5.91) genotypes were strongly associated with asthma. Moreover, individuals with the TT genotype had significantly higher serum IL-4 levels (P < 0.001).

Conclusion: The IL4 rs2243250 polymorphism is associated with increased asthma susceptibility and elevated serum IL-4 levels in the Iraqi population. Mycoplasma pneumoniae infection also appears to be a significant contributing factor. Larger-scale studies are warranted to confirm these findings and further explore the role of this infection in asthma pathogenesis.

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伊拉克人群il - 4 （Rs2243250）基因变异与肺炎支原体感染与哮喘易感性的关系

背景/目的：哮喘是一种受遗传和环境因素共同影响的多因素疾病。本研究旨在探讨IL-4基因多态性（rs2243250）与哮喘易感性以及血清IL-4水平之间的关系。此外，它还探讨了肺炎支原体感染作为哮喘的潜在危险因素。方法：共纳入118人，包括60例哮喘患者和58例健康对照。采用等位基因特异性PCR （AS-PCR）对IL4 rs2243250进行基因分型。血清学评估既往肺炎支原体感染，ELISA检测血清IL-4水平。结果：在年龄、性别或居住地方面，各组间无显著差异。吸烟（OR: 7.85, P = 0.001）和哮喘家族史（OR: 5.33, P = 0.004）被确定为显著危险因素。肺炎支原体感染在哮喘患者中的流行率（41.7%）明显高于对照组，与哮喘风险有很强的相关性（OR: 8.75, P < 0.0001）。rs2243250基因型频率差异显著：CC （36.7% vs. 68.9%）、CT （41.7% vs. 24.2%）和TT （21.6% vs. 6.9%）患者与对照组。T等位基因在患者中的出现频率（42.5%）高于对照组（18.97%），增加了哮喘风险（OR: 3.16, P = 0.0001）。CT （OR: 3.25）和TT （OR: 5.91）基因型均与哮喘密切相关。此外，TT基因型个体血清IL-4水平显著高于对照组（P < 0.001）。结论：IL-4 rs2243250多态性与伊拉克人群哮喘易感性增加和血清IL-4水平升高有关。肺炎支原体感染似乎也是一个重要因素。需要更大规模的研究来证实这些发现，并进一步探索这种感染在哮喘发病机制中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cellular Physiology and Biochemistry 生物-生理学

CiteScore

5.80

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Cellular Physiology and Biochemistry is a multidisciplinary scientific forum dedicated to advancing the frontiers of basic cellular research. It addresses scientists from both the physiological and biochemical disciplines as well as related fields such as genetics, molecular biology, pathophysiology, pathobiochemistry and cellular toxicology & pharmacology. Original papers and reviews on the mechanisms of intracellular transmission, cellular metabolism, cell growth, differentiation and death, ion channels and carriers, and the maintenance, regulation and disturbances of cell volume are presented. Appearing monthly under peer review, Cellular Physiology and Biochemistry takes an active role in the concerted international effort to unravel the mechanisms of cellular function.