Magnesium and Zinc Dose-Dependently Stabilize Rat Peritoneal Mast Cells and Enhance the Effects of Adrenaline.

IF 2.5 Q3 CELL BIOLOGY

Cellular Physiology and Biochemistry Pub Date : 2025-07-15 DOI:10.33594/000000793

Itsuro Kazama, Hiroyuki Sonobe, Junko Shida

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引用次数: 0

Abstract

Background/aims: Magnesium and zinc are vital trace elements found in numerous foods and dietary supplements. In addition to their antioxidant, anticancer, antibacterial, and anti-inflammatory effects, clinical research has suggested that they possess anti-allergic properties.

Methods: Using differential-interference contrast (DIC) microscopy, we examined the effects of magnesium chloride (MgCl2) and zinc chloride (ZnCl2) on rat peritoneal mast cell degranulation. We also examined their effects in conjunction with adrenaline, the first-choice drug for anaphylaxis treatment.

Results: Both MgCl2 and ZnCl2 reduced the number of degranulating mast cells in a dose-dependent manner. MgCl2 significantly decreased the number of degranulating mast cells at concentrations of 50 mM or higher, whereas ZnCl2 achieved similar effects at much lower concentrations of 25 µM or more. These levels of MgCl2 or ZnCl2 enhanced the inhibitory effects of 1 mM adrenaline on mast cell degranulation. Additionally, pharmacological inhibition of the transient receptor potential cation channel subfamily M member 7 (TRPM7) by NS8593 reduced the number of degranulating mast cells in a dose-dependent manner.

Conclusion: This study is the first to provide in vitro evidence that magnesium and zinc stabilize mast cells in a dose-dependent manner and also enhance the effects of adrenaline. TRPM7, which has higher permeability to zinc ions than to magnesium ions, may contribute to the stronger mast cell-stabilizing properties of zinc.

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镁和锌剂量依赖性稳定大鼠腹膜肥大细胞并增强肾上腺素的作用。

背景/目的：镁和锌是在许多食物和膳食补充剂中发现的重要微量元素。除了抗氧化、抗癌、抗菌和抗炎作用外，临床研究表明它们还具有抗过敏的特性。方法：采用差干涉对比（DIC）显微镜观察氯化镁（MgCl2）和氯化锌（ZnCl2）对大鼠腹膜肥大细胞脱颗粒的影响。我们还检查了它们与肾上腺素（过敏反应治疗的首选药物）联合使用的效果。结果：MgCl2和ZnCl2均呈剂量依赖性地减少脱颗粒肥大细胞的数量。MgCl2在浓度为50 mM或更高时显著减少脱颗粒肥大细胞的数量，而ZnCl2在浓度为25 μ M或更高时达到相似的效果。这些水平的MgCl2或ZnCl2增强了1mm肾上腺素对肥大细胞脱颗粒的抑制作用。此外，NS8593对瞬时受体电位阳离子通道亚家族M成员7 （TRPM7）的药理学抑制以剂量依赖的方式减少脱颗粒肥大细胞的数量。结论：本研究首次在体外提供了镁锌稳定肥大细胞的剂量依赖性，并增强肾上腺素作用的证据。TRPM7对锌离子的渗透性高于对镁离子的渗透性，这可能有助于锌具有更强的肥大细胞稳定特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cellular Physiology and Biochemistry 生物-生理学

CiteScore

5.80

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Cellular Physiology and Biochemistry is a multidisciplinary scientific forum dedicated to advancing the frontiers of basic cellular research. It addresses scientists from both the physiological and biochemical disciplines as well as related fields such as genetics, molecular biology, pathophysiology, pathobiochemistry and cellular toxicology & pharmacology. Original papers and reviews on the mechanisms of intracellular transmission, cellular metabolism, cell growth, differentiation and death, ion channels and carriers, and the maintenance, regulation and disturbances of cell volume are presented. Appearing monthly under peer review, Cellular Physiology and Biochemistry takes an active role in the concerted international effort to unravel the mechanisms of cellular function.