Cellular Microbiology最新文献

{"title":"Ferroptosis Is a Potential Therapeutic Target for Pulmonary Infectious Diseases","authors":"Yurong Zhang,&nbsp;Dianlun Qian,&nbsp;Xiangfeng Bai,&nbsp;Shibo Sun","doi":"10.1155/2023/3875897","DOIUrl":"10.1155/2023/3875897","url":null,"abstract":"<div>\u0000 <p>Ferroptosis is a new type of iron-dependent cell death caused by lipid peroxide (LPO) accumulation and involved in disease of pulmonary infection. The dysregulation of iron metabolism, the accumulation of LPO, and the inactivation and consumption of glutathione peroxidase 4 (GPX4) are the crucial cause of ferroptosis. Pulmonary infectious diseases caused by Pseudomonas aeruginosa (PA), Mycobacterium tuberculosis (MTB), and severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) are associated with ferroptosis. Ferroptosis may be a potential therapeutic target for pulmonary infectious diseases. However, the mechanisms by which these infections are involved in ferroptosis and whether pulmonary infectious diseases caused by Staphylococcus aureus, Klebsiella pneumoniae, and Leishmania spp are related to ferroptosis are unclear. Accordingly, more researches are needed.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2023 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/3875897","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44373940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"G1 Cell Cycle Arrest Is Induced by the Fourth Extracellular Loop of Meningococcal PorA in Epithelial and Endothelial Cells","authors":"Matthew Vassey,&nbsp;Rininta Firdaus,&nbsp;Akhmed Aslam,&nbsp;Lee M. Wheldon,&nbsp;Neil J. Oldfield,&nbsp;Dlawer A. A. Ala’Aldeen,&nbsp;Karl G. Wooldridge","doi":"10.1155/2023/7480033","DOIUrl":"10.1155/2023/7480033","url":null,"abstract":"<div>\u0000 <p><i>Neisseria meningitidis</i> is the most frequent cause of bacterial meningitis and is one of the few bacterial pathogens that can breach the blood-brain barrier (BBB). The 37/67 kDa laminin receptor (LamR) was previously identified as a receptor mediating meningococcal binding to rodent and human brain microvascular endothelial cells, which form part of the BBB. The meningococcal surface proteins PorA and PilQ were identified as ligands for this receptor. Subsequently, the fourth extracellular loop of PorA (PorA-Loop4) was identified as the LamR-binding moiety. Here, we show that PorA-Loop4 targets the 37 kDa laminin receptor precursor (37LRP) on the cell surface by demonstrating that deletion of this loop abrogates the recruitment of 37LRP under meningococcal colonies. Using a circularized peptide corresponding to PorA-Loop4, as well as defined meningococcal mutants, we demonstrate that host cell interaction with PorA-Loop4 results in perturbation of p-CDK4 and Cyclin D1. These changes in cell cycle control proteins are coincident with cellular responses including inhibition of cell migration and a G₁ cell cycle arrest. Modulation of the cell cycle of host cells is likely to contribute to the pathogenesis of meningococcal disease.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2023 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/7480033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47272920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Akkermansia muciniphila Ameliorates Lung Injury in Smoke-Induced COPD Mice by IL-17 and Autophagy","authors":"Li Zhang,&nbsp;Junjuan Lu,&nbsp;Caihong Liu","doi":"10.1155/2023/4091825","DOIUrl":"10.1155/2023/4091825","url":null,"abstract":"<div>\u0000 <p><i>Objective</i>. Smoking is a primary hazard factor for chronic obstructive pulmonary disease (COPD), which induced a decrease in intestinal <i>Akkermansia muciniphila</i> abundance and Th17 imbalance in COPD. This study analyzed the changes of gut microbiota metabolism and <i>Akkermansia</i> abundance in patients with smoking-related COPD and explored the potential function of <i>Akkermansia muciniphila</i> in smoke-induced COPD mice. <i>Methods</i>. Gut microbiota diversity and metabolic profile were analyzed by 16S rRNA sequence and metabolomics in COPD patients. The IL-1<i>β</i>, IL-17, TNF-<i>α</i>, and IL-6 levels were tested by ELISA. Lung tissue damage was observed by HE staining. The expression of cleave-caspase 3, trophoblast antigen 2 (TROP2), and LC3 in lung tissues were analyzed by IHC or IF. The p-mTOR, mTOR, p62, and LC3 expression in lung tissues were tested by western blot. <i>Results</i>. The levels of IL-17, IL-1<i>β</i>, TNF-<i>α</i>, and IL-6 in the peripheral blood of COPD patients increased significantly. The number and alpha diversity of gut microbiota were decreased in COPD patients. The abundance of <i>Akkermansia muciniphila</i> in gut of COPD patients was decreased, and the metabolic phenotype and retinol metabolism were changed. In the retinol metabolism, the retinol and retinal were significantly changed. <i>Akkermansia muciniphila</i> could improve the alveolar structure and inflammatory cell infiltration in lung tissue, reduce the IL-17, TNF-<i>α</i>, and IL-6 levels in peripheral blood, promote the p-mTOR expression, and inhibit the expression of autophagy-related proteins in smoke-induced COPD mice. <i>Conclusion</i>. The number and alpha diversity of gut microbiota were decreased in patients with smoking-related COPD, accompanied by decreased abundance of <i>Akkermansia muciniphila</i>, and altered retinol metabolism function. Gut <i>Akkermansia muciniphila</i> ameliorated lung injury in smoke-induced COPD mice by inflammation and autophagy.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2023 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/4091825","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42165692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockout of Noxa with CRISPR/Cas9 Increases Host Resistance to Influenza Virus Infection","authors":"Ao Zhou,&nbsp;Wenhua Zhang,&nbsp;Baoxin Wang,&nbsp;Xia Dong,&nbsp;Jing Zhang,&nbsp;Hongbo Chen","doi":"10.1155/2023/3877614","DOIUrl":"10.1155/2023/3877614","url":null,"abstract":"<div>\u0000 <p>The influenza virus induces cellular apoptosis during viral propagation, and controlling this virus-induced apoptosis process has been shown to have significant antiviral effects. The proapoptotic BH3-only protein Noxa is a strong inducer of apoptosis that can be activated by this virus, suggesting that Noxa has the potential as an anti-influenza target. To assess the value of Noxa as an antiviral target, we utilized CRISPR/Cas9 technology to produce a Noxa-knockout cell line. We found that the knockout of Noxa resulted in a dramatic reduction in the cytopathic effect induced by the influenza virus. Moreover, Noxa knockout decreased the expression of influenza viral proteins (NP, M2, HA, and NS2). In addition, Noxa deficiency triggered a complete autophagic flux to weaken influenza virus-induced autophagosome accumulation, indicating that Noxa may be a promising antiviral target for controlling influenza virus infections.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2023 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/3877614","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42002335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycobacterium avium Infection of Multinucleated Giant Cells Reveals Association of Bacterial Survival to Autophagy and Cholesterol Utilization","authors":"Jayanthi J. Joseph,&nbsp;Amy Leestemaker-Palmer,&nbsp;Soheila Kazemi,&nbsp;Lia Danelishvili,&nbsp;Luiz E. Bermudez","doi":"10.1155/2023/5064371","DOIUrl":"10.1155/2023/5064371","url":null,"abstract":"<div>\u0000 <p><i>Mycobacterium avium</i> subsp. <i>hominissuis</i> (<i>M. avium</i>) is an opportunistic environmental pathogen that typically infects patients with existing lung conditions such as cystic fibrosis or COPD. Pulmonary <i>M. avium</i> infection generates peribronchial granulomas that contain infected macrophages and multinucleated giant cells (MGCs). While granuloma formation with MGCs is a common feature of mycobacterial infection, the role of MGCs within the granulomas as well as in the host-pathogen interaction is poorly understood. To shed light on the role of MGCs, we established a novel <i>in vitro</i> model utilizing THP-1 cells stimulated with a combination of IFN-<i>γ</i> and TNF-<i>α</i>. In this study, we show that MGCs can take up <i>M. avium</i>, which replicates intracellularly before leaving the cell. Bacteria that escape the MGC exhibit a highly invasive phenotype, which warrants further evaluation. Characterization of MGCs with transmission electron microscopy revealed an accumulation of cytoplasmic lipid droplets, autophagic activity, and multiple nuclei. Autophagy markers are upregulated in both uninfected and infected MGCs early in infection, measured by RT-qPCR analysis of Beclin-1 and LC3. Inhibition of autophagy with siRNA significantly reduced <i>M. avium</i> survival significantly in THP-1 macrophages. Depletion of host cholesterol and sphingomyelin in MGCs also resulted in decreased survival of <i>M. avium</i>. These processes potentially contribute to the formation of a supportive intracellular environment for the pathogen. Collectively, our results suggest that <i>M. avium</i> is adapted to replicate in MGCs and utilize them as a springboard for local spread.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2023 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/5064371","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42021136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kluyveromyces marxianus Ameliorates High-Fat-Diet-Induced Kidney Injury by Affecting Gut Microbiota and TLR4/NF-κB Pathway in a Mouse Model","authors":"Na Li,&nbsp;Guanjie Zhao,&nbsp;Mingzhu Xu","doi":"10.1155/2023/2822094","DOIUrl":"10.1155/2023/2822094","url":null,"abstract":"<div>\u0000 <p><i>Objectives</i>. The effects of <i>Kluyveromyces marxianus</i> on high-fat diet- (HFD-) induced kidney injury (KI) were explored. <i>Methods</i>. HFD-induced KI model was established using male C57BL/6 mice and treated with <i>K. marxianus</i> JLU-1016 and acid-resistant (AR) strain JLU-1016A. Glucose tolerance was evaluated via an oral glucose tolerance test (OGTT). KI was measured using Hematoxylin and Eosin (H&amp;E) staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analysis. The chemical indexes were analyzed, including lipid profiles, inflammatory cytokines, and creatinine. The levels of Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-<i>κ</i>B) or phospho-NF-<i>κ</i>B p65 (Ser536) and alpha inhibitor of NF-<i>κ</i>B (I<i>κ</i>B<i>α</i>) were measured using qPCR and Western blot. The gut microbiota was sequenced using high-throughput sequencing. <i>Results</i>. HFD induction increased OGTT value, KI severity, oxidative stress, inflammatory cytokines, oxidative stress, apoptotic rate, creatinine levels, and the expression of TLR4/NF-<i>κ</i>B, phospho-NF-<i>κ</i>B p65 (Ser536), and I<i>κ</i>B<i>α</i> deteriorated lipid profiles (<i>P</i> &lt; 0.05) and reduced gut microbiota abundance. <i>K. marxianus</i> treatment ameliorated HFD-induced metabolic disorders and reversed these parameters (<i>P</i> &lt; 0.05). Compared with the control, HFD induction increased the proportion of <i>Firmicutes</i> but reduced the proportion of <i>Bacteroidetes</i> and <i>Lactobacillus</i>. <i>K. marxianus</i> JLU-1016 and AR strain JLU-1016A treatments improved gut microbiota by reducing the proportion of <i>Firmicutes</i> and increasing the proportion of <i>Bacteroidetes</i> and <i>Lactobacillus</i> in the KI model (<i>P</i> &lt; 0.0001). <i>Helicobacter</i> has been identified with many infectious diseases and was increased after HFD induction and inhibited after <i>K. marxianus</i> JLU-1016 and AR strain JLU-1016A treatments. The strain JLU-1016A exhibited better results possibly with acid-tolerance properties to pass through an acidic environment of the stomach. <i>Conclusions</i>. <i>K. marxianus</i> may have a beneficial effect on KI by improving gut microbiota and inhibiting TLR4/NF-<i>κ</i>B pathway activation.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2023 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/2822094","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46914924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial Cells","authors":"Hao Yin,&nbsp;Jiaoli Zhu,&nbsp;Yi Jiang,&nbsp;Yijing Mao,&nbsp;Chenquan Tang,&nbsp;Hui Cao,&nbsp;Yufang Huang,&nbsp;Huijun Zhu,&nbsp;Jianping Luo,&nbsp;Qingjiang Jin,&nbsp;Qinglei Jin,&nbsp;Yi Xue,&nbsp;Xin Wang","doi":"10.1155/2023/3201540","DOIUrl":"10.1155/2023/3201540","url":null,"abstract":"<div>\u0000 <p>In clinical practice, urinary tract infections (UTIs) are second only to respiratory infections in terms of infectious diseases. In recent years, drug resistance of <i>Escherichia coli</i> (<i>E. coli</i>) has increased significantly. The therapeutic effects of Shionone on UTI were assessed by modelling UTI in SD rats and SV-HUC-1 cells with <i>E. coli</i> solution. After treatment of Shionone, the UTI rat model showed a decrease in wet weight/body weight of bladder, as well as a reduction in cellular inflammatory infiltration of bladder tissue and a decrease in urinary levels of IL-6, IL-1<i>β</i>, and TNF-<i>α</i>. In addition, the levels of proinflammatory factors were significantly reduced in a dose-dependent manner in UTI cell model treated with different doses of Shionone (5, 10, and 20 <i>μ</i>g/kg). The results of immunofluorescence analysis in both <i>in vivo</i> and <i>in vitro</i> experiments revealed that Shionone reduced bacterial load and the number of <i>E. coli</i> colonies growing on the plates was greatly reduced. These results suggested that Shionone has a good therapeutic effect on UTI, achieved by reducing bacterial load in bladder epithelial cells. The data presented here provide a basis for further research into the treatment of UTI.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2023 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/3201540","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46164264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liver Microbiome in Healthy Rats: The Hidden Inhabitants of Hepatocytes","authors":"Xiao Wei Sun,&nbsp;Hua Zhang,&nbsp;Xiao Zhang,&nbsp;Peng Fei Xin,&nbsp;Xue Gao,&nbsp;Hong Rui Li,&nbsp;Cai Yun Zhou,&nbsp;Wen Min Gao,&nbsp;Xuan Xuan Kou,&nbsp;Jian Gang Zhang","doi":"10.1155/2023/7369034","DOIUrl":"10.1155/2023/7369034","url":null,"abstract":"<div>\u0000 <p>The tumor and tissue microbiota of human beings have recently been investigated. Gut permeability is known as a possible resource for the positive detection of tissue bacteria. Herein, we report that microbiota were detected in high abundance in the hepatocytes of healthy rats and that they were shared with the gut microbiota to an extent. We assessed male Sprague Dawley (SD) rats for the 16S ribosomal ribonucleic acid (rRNA) gene. After the rats were sacrificed by blood drainage from the portal vein, we extracted total deoxyribonucleic acid (DNA) from their ileal and colonic contents and liver tissues. The V3–V4 region of the 16S rRNA gene was amplified by polymerase chain reaction (PCR) and sequenced using an Illumina HiSeq 2500 platform. Sequences were assigned taxonomically by the SILVA database. We also detected bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA) in situ using immunofluorescence (IF) and western blotting and the 16S rRNA gene using fluorescent in situ hybridization (FISH). In the livers of six rats, we detected 54,867.50 ± 6450.03 effective tags of the 16S rRNA gene and clustered them into 1003 kinds of operational taxonomic units (OTUs; 805.67 ± 70.14, 729–893). Rats showed conservation of bacterial richness, abundance, and evenness. LPS and the 16S rRNA gene were detected in the nuclei of hepatocytes. The main function composition of the genomes of annotated bacteria was correlated with metabolism (79.92 ± 0.24<i>%</i>). Gram negativity was about 1.6 times higher than gram positivity. The liver microbiome was shared with both the small and large intestines but showed significantly higher richness and evenness than the gut microbiome, and the <i>β</i>-diversity results showed that the liver microbiome exhibited significantly higher similarity than the small and large intestines (<i>P</i> &lt; 0.05). Our results suggest that the bacteria in the liver microbiome are hidden intracellular inhabitants in healthy rat livers.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2023 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/7369034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45324244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial Cells","authors":"Geunhye Hong,&nbsp;Cadi Davies,&nbsp;Zahra Omole,&nbsp;Janie Liaw,&nbsp;Anna D. Grabowska,&nbsp;Barbara Canonico,&nbsp;Nicolae Corcionivoschi,&nbsp;Brendan W. Wren,&nbsp;Nick Dorrell,&nbsp;Abdi Elmi,&nbsp;Ozan Gundogdu","doi":"10.1155/2023/3286330","DOIUrl":"10.1155/2023/3286330","url":null,"abstract":"<div>\u0000 <p><i>Campylobacter jejuni</i> is the major bacterial cause of foodborne gastroenteritis worldwide. Mechanistically, how this pathogen interacts with intrinsic defence machinery of human intestinal epithelial cells (IECs) remains elusive. To address this, we investigated how <i>C. jejuni</i> counteracts the intracellular and extracellular reactive oxygen species (ROS) in IECs. Our work shows that <i>C. jejuni</i> differentially regulates intracellular and extracellular ROS production in human T84 and Caco-2 cells. <i>C. jejuni</i> downregulates the transcription and translation of nicotinamide adenine dinucleotide phosphate (NAPDH) oxidase (NOX1), a key ROS-generating enzyme in IECs and antioxidant defence genes <i>CAT</i> and <i>SOD1</i>. Furthermore, inhibition of <i>NOX1</i> by diphenylene iodonium (DPI) and siRNA reduced <i>C. jejuni</i> ability to interact, invade, and intracellularly survive within T84 and Caco-2 cells. Collectively, these findings provide mechanistic insight into how <i>C. jejuni</i> modulates the IEC defence machinery.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2023 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/3286330","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135555374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fecal Microbiome Does Not Represent Whole Gut Microbiome","authors":"Ji-Seon Ahn,&nbsp;Enkhchimeg Lkhagva,&nbsp;Sunjun Jung,&nbsp;Hyeon-Jin Kim,&nbsp;Hea-Jong Chung,&nbsp;Seong-Tshool Hong","doi":"10.1155/2023/6868417","DOIUrl":"10.1155/2023/6868417","url":null,"abstract":"<div>\u0000 <p>The current gut microbiome research relies on the fecal microbiome under the assumption that the fecal microbiome represents the microbiome of the entire gastrointestinal (GI) tract. However, there have been growing concerns about using feces as a proxy to study the gut microbiome. Here, we comprehensively analyzed the composition of microbiome and metabolites in the feces and at 14 different locations of GI tracts of genetically homogenous sibling pigs to evaluate the validity of using feces as a proxy to the whole gut microbiome. The composition of intestinal microbes constituting the gut microbiome at each intestinal content and feces and their metabolic compositions were thoroughly investigated through metagenome sequencing and an ultraperformance LC-MS/MS, respectively. The fluctuation in the composition of the microbiome in the stomach and the small intestine became stabilized from the large intestine to feces and was able to be categorized into 3 groups. The taxonomic <i>α</i>-diversities measured by ACE (abundance-based coverage estimator) richness and Shannon diversity indicated that the microbiome in the large intestine was much more diverse than those of the small intestine and feces. The highly independent intestinal microbes in the stomach and the small intestine became flourished in the large intestine and converged into a community with tightly connected networks. <i>β</i>-Diversity analyses by NMDS plots, PCA, and unsupervised hierarchical clustering all showed that the diversities of microbiome compositions were lowest in feces while highest in the large intestine. In accordance with fluctuation of the composition of gut microbiome along with the GI tract, the metabolic composition also completely differed in a location-specific manner along with the GI tract. Comparative analysis of the fecal microbiome and metabolites with those of the whole GI tract indicated that fecal microbiome is insufficient to represent the whole gut microbiome.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2023 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/6868417","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48341420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}