CRISPR/Cas9敲除Noxa增强宿主对流感病毒感染的抵抗力

IF 2.6 2区生物学 Q3 CELL BIOLOGY

Cellular Microbiology Pub Date : 2023-02-16 DOI:10.1155/2023/3877614

Ao Zhou, Wenhua Zhang, Baoxin Wang, Xia Dong, Jing Zhang, Hongbo Chen

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引用次数: 0

摘要

流感病毒在病毒繁殖过程中诱导细胞凋亡，控制这种病毒诱导的细胞凋亡过程已被证明具有显著的抗病毒作用。只有BH3的促凋亡蛋白Noxa是一种强大的细胞凋亡诱导剂，可以被这种病毒激活，这表明Noxa具有抗流感靶点的潜力。为了评估Noxa作为抗病毒靶标的价值，我们利用CRISPR/Cas9技术生产了Noxa敲除细胞系。我们发现，敲除Noxa导致流感病毒诱导的细胞病变效应显著降低。此外，Noxa敲除降低了流感病毒蛋白（NP、M2、HA和NS2）的表达。此外，Noxa缺乏引发了完全的自噬流量，以削弱流感病毒诱导的自噬体积累，这表明Noxa可能是控制流感病毒感染的一个有前途的抗病毒靶点。

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Knockout of Noxa with CRISPR/Cas9 Increases Host Resistance to Influenza Virus Infection

The influenza virus induces cellular apoptosis during viral propagation, and controlling this virus-induced apoptosis process has been shown to have significant antiviral effects. The proapoptotic BH3-only protein Noxa is a strong inducer of apoptosis that can be activated by this virus, suggesting that Noxa has the potential as an anti-influenza target. To assess the value of Noxa as an antiviral target, we utilized CRISPR/Cas9 technology to produce a Noxa-knockout cell line. We found that the knockout of Noxa resulted in a dramatic reduction in the cytopathic effect induced by the influenza virus. Moreover, Noxa knockout decreased the expression of influenza viral proteins (NP, M2, HA, and NS2). In addition, Noxa deficiency triggered a complete autophagic flux to weaken influenza virus-induced autophagosome accumulation, indicating that Noxa may be a promising antiviral target for controlling influenza virus infections.

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来源期刊

Cellular Microbiology 生物-微生物学

CiteScore

9.70

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Cellular Microbiology aims to publish outstanding contributions to the understanding of interactions between microbes, prokaryotes and eukaryotes, and their host in the context of pathogenic or mutualistic relationships, including co-infections and microbiota. We welcome studies on single cells, animals and plants, and encourage the use of model hosts and organoid cultures. Submission on cell and molecular biological aspects of microbes, such as their intracellular organization or the establishment and maintenance of their architecture in relation to virulence and pathogenicity are also encouraged. Contributions must provide mechanistic insights supported by quantitative data obtained through imaging, cellular, biochemical, structural or genetic approaches.