{"title":"High-Performance Oxidation and Nanomolar Detection of Phenylhydrazine Using a 6-Hydroxyflavone-Based Molecular Electrocatalyst Functionalized Multiwalled Carbon Nanotube in Batch Injection Analysis.","authors":"Valisireddy Lavanya, Kannappan Santhakumar, Annamalai Senthil Kumar","doi":"10.1002/open.202500140","DOIUrl":"https://doi.org/10.1002/open.202500140","url":null,"abstract":"<p><p>The development of simple and rapid methods for preparing redox-active molecular catalyst-functionalized carbon electrodes for electrocatalytic applications is a significant research area. 6-Hydroxyflavone (HFLA), a naturally occurring flavonoid with known anxiolytic properties, also acts as a noncompetitive inhibitor of cytochrome. This study focuses on the in situ functionalization of multiwalled carbon nanotubes (MWCNTs) with redox-active HFLA, resulting in a modified electrode denoted as GCE/MWCNT@HFLA-Redox, where HFLA-Redox represents the redox-active product of HFLA. The constructed-modified electrode exhibits a well-defined and stable surface-confined redox response at E° of 0.55 V versus Ag/AgCl, with a surface excess of 6.26 × 10<sup>-9</sup> mol cm<sup>-2</sup> in a pH 2 KCl-HCl solution. The modified electrode is characterized by Fourier transform infrared, Raman, UV-vis, field-emission scanning electron microscopy, high-resolution mass spectrometry (organic extract), and control electrochemical studies. This GCE/MWCNT@HFLA-Redox electrode selectively oxidizes phenylhydrazine (PhHyd) in a pH 2 KCl-HCl solution. A screen-printed-modified electrode facilitates highly selective electrocatalytic oxidation of PhHyd via amperometric i-t measurements and batch injection analysis, without interference from hydrazine or other common electroactive species. This method exhibits an excellent linear calibration curve (200 nM to 2 μM), demonstrating a high-current sensitivity of 0.413 μA μM<sup>-1</sup> cm<sup>-2</sup> and a detection limit of 7 nM (signal-to-noise ratio of 3).</p>","PeriodicalId":9831,"journal":{"name":"ChemistryOpen","volume":" ","pages":"e2500140"},"PeriodicalIF":2.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemistryOpenPub Date : 2025-06-09DOI: 10.1002/open.202400521
Essi Rytkönen, Nina Hakulinen, Janne Jänis, Juha Rouvinen
{"title":"Exploring Unspecific Peroxygenase Selectivity with Diverse Hydrocarbon Substrates.","authors":"Essi Rytkönen, Nina Hakulinen, Janne Jänis, Juha Rouvinen","doi":"10.1002/open.202400521","DOIUrl":"https://doi.org/10.1002/open.202400521","url":null,"abstract":"<p><p>Unspecific peroxygenases (UPOs) are enzymes capable of oxidising various substrates using hydrogen peroxide as a co-substrate. UPOs have gained interest due to their broad substrate specificity, which includes relatively inexpensive precursors for valuable compounds, such as pharmaceuticals and chemical building blocks. In this study, the activity of a panel of 30 UPOs toward aliphatic and aromatic hydrocarbons was screened to assess variation in substrate selectivity. Most of the studied UPOs were able to oxidise the substrates efficiently, producing 2-5 products via hydrogen abstraction or epoxidation. Overall trends were observed, such as the preference for aromatic oxidation over benzylic hydroxylation in the case of toluene, while the opposite was observed with ethylbenzene. Different UPOs could also be categorized based on their reaction profiles with all substrates. Selectivity was generally low, as oxyfunctionalization reactions led to mixtures of products that were further oxidised. However, styrene and cyclohexene were converted rather exclusively to their epoxide products. The results indicate that the substrate scope of UPOs includes various types of hydrocarbons, which are oxidised depending on the individual enzyme's active site. They also hold potential for producing chemicals for industrial purposes more sustainably, but their selectivity should first be improved through protein engineering.</p>","PeriodicalId":9831,"journal":{"name":"ChemistryOpen","volume":" ","pages":"e202400521"},"PeriodicalIF":2.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemistryOpenPub Date : 2025-06-09DOI: 10.1002/open.202500042
Shilin Zhang, Yanan Zhu, Hailiang Ni, Ping Hu, Yibin Sun
{"title":"A Fuel-Driven Lock-and-Key System.","authors":"Shilin Zhang, Yanan Zhu, Hailiang Ni, Ping Hu, Yibin Sun","doi":"10.1002/open.202500042","DOIUrl":"https://doi.org/10.1002/open.202500042","url":null,"abstract":"<p><p>This study introduces a fuel-driven lock-and-key system based on the interaction between crown ether and ammonium ion. In this simple model system, a key-like molecule with an amino group functions as the key, while 15-crown-5 serves as the lock. The chemical fuel, 2-cyano-2-phenylpropanoic acid, protonates the key, transitioning it from its deprotonated state to a protonated state, enabling it to bind to the lock. Upon fuel consumption, the protonated key reverts to its deprotonated state, causing the dissociation from the lock. This cycle is reversible and can be repeated at least three times. We hope that this intuitive lock-and-key system can provide a clearer understanding of energy-driven molecular recognition and offer valuable insights into the design and development of energy-driven molecular systems based on molecular recognition.</p>","PeriodicalId":9831,"journal":{"name":"ChemistryOpen","volume":" ","pages":"e2500042"},"PeriodicalIF":2.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemistryOpenPub Date : 2025-06-09DOI: 10.1002/open.202500261
Ahmed A Abd-Rabou, Ahmed M Youssef, Mohamed I El-Khonezy, Soheir E Kotob, Mohamed S Kishta
{"title":"Anti-Inflammatory/Antioxidant Features of Adipose Tissue Mesenchymal Stem Cells Conditioned Media for Doped TiO<sub>2</sub> Nanoparticles in Induced Inflammation.","authors":"Ahmed A Abd-Rabou, Ahmed M Youssef, Mohamed I El-Khonezy, Soheir E Kotob, Mohamed S Kishta","doi":"10.1002/open.202500261","DOIUrl":"https://doi.org/10.1002/open.202500261","url":null,"abstract":"<p><p>The fundamental purpose of this work is to determine the anti-inflammatory/antioxidant activity of stem cell conditioned medium enhanced by mono- or dual-doped TiO<sub>2</sub> nanoparticles. The transmission electron microscope, X-ray diffraction, and energy-dispersive X-ray analyses are used to characterize the nanoparticles. Isolation/characterization of adipose tissue mesenchymal stem cells (AD-MSCs) is done. In vitro assays reveal that protein denaturation and proteinase induction are significantly increased with lipopolysaccharide (LPS) comparable to control, while treatments significantly decrease the induction compared to LPS. In vitro assays reveal decreasing in hydroxyl radical scavenging and DPPH radical scavenging activities with LPS, while treatments significantly increase the activity compared to LPS. Induction with LPS decreases in vitro catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) expression levels and enzyme activities are significantly compared to controls, while treatments significantly increase the CAT expression compared to LPS. Induction with LPS increases the in vitro interleukin 4 (IL4), IL6, IL10, and tumor necrosis factor α expression levels, and their activities significantly decline with treatment compared to controls, while treatments significantly decline expression compared to LPS. The anti-inflammatory and antioxidant properties of AD-MSC-conditioned medium enhanced with mono- or dual-doped TiO<sub>2</sub> nanoparticles are identified in this investigation. To sum up, the work has shown that mono- and dual-doped TiO<sub>2</sub> can inhibit the inflammation caused by LPS in vitro.</p>","PeriodicalId":9831,"journal":{"name":"ChemistryOpen","volume":" ","pages":"e2500261"},"PeriodicalIF":2.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemistryOpenPub Date : 2025-06-08DOI: 10.1002/open.202500114
Dipu Bishwas, Md Shimul Bhuia, Salehin Sheikh, Mohammed Alfaifi, Abdul Malik, Nikhat J Siddiqui, Divya Jain, Mehedi Hasan Bappi, Muhammad Torequl Islam
{"title":"Evaluation of the Sedative Activity of Naringenin: In Vivo Study with Pharmacokinetics and Molecular Docking Insights.","authors":"Dipu Bishwas, Md Shimul Bhuia, Salehin Sheikh, Mohammed Alfaifi, Abdul Malik, Nikhat J Siddiqui, Divya Jain, Mehedi Hasan Bappi, Muhammad Torequl Islam","doi":"10.1002/open.202500114","DOIUrl":"https://doi.org/10.1002/open.202500114","url":null,"abstract":"<p><p>This research is designed to investigate the sedative effects of naringenin (NAR) and diazepam against thiopental sodium-induced sleeping mice. NAR (5 and 10 mg kg), diazepam (DZP) (2 mg kg), flumazenil (FLN) (0.1 mg kg), and their combinations are administered intraperitoneally to mice. After 30 min, sleep is induced with intraperitoneal thiopental sodium (20 mg kg), and sleep latency and duration are measured. In silico analysis investigates the role of GABA receptors. NAR significantly reduces sleep latency and prolongs sleep duration in a dose-dependent manner, with the combination of NAR-10 and DZP-2 showing a synergistic effect. The combination of the antagonist (FLN) (NAR-10 and FLN-0.1) indicates that latency increases and sleep duration decreases compared to NAR-10 alone. Furthermore, in silico docking studies corroborates these results, demonstrating a significant binding affinity of NAR (-8.3 kcal mol), which is comparable to the standard ligand DZP (-8.7 kcal mol) and FLN (-7.0 kcal mol) for the GABA<sub>A</sub> (6X3X) receptor, indicating a GABAergic mechanism. Pharmacokinetics and toxicity evaluations confirm NAR's potential as a safe therapeutic agent, with a high LD50 (2000 mg kg) and minimal toxicity. These findings highlight NAR's potential as a GABAergic sedative agent, requiring further research before being used clinically to treat sleep disorders.</p>","PeriodicalId":9831,"journal":{"name":"ChemistryOpen","volume":" ","pages":"e2500114"},"PeriodicalIF":2.5,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemistryOpenPub Date : 2025-06-08DOI: 10.1002/open.202500175
Muhammad Naseer, Muhammad Adil, Sarir Ahmad, Mikhlid H Almutari, Abdulwahed Fahad Alrefaei, Sajid Ali, Fayyaz Asad
{"title":"Gas Chromatography-Mass Spectrometry Analysis, Genoprotective, and Antioxidant Potential of Curio Radicans (L. f.) P.V. Heath.","authors":"Muhammad Naseer, Muhammad Adil, Sarir Ahmad, Mikhlid H Almutari, Abdulwahed Fahad Alrefaei, Sajid Ali, Fayyaz Asad","doi":"10.1002/open.202500175","DOIUrl":"https://doi.org/10.1002/open.202500175","url":null,"abstract":"<p><p>Natural substances play a crucial role in modern medicine by targeting cellular pathways associated with diseases. Medicinal plants, rich in bioactive compounds, offer promising therapeutic potential. This study examines the phytochemicals and biological activity of Curio radicans extracts. Gas chromatography-mass spectrometry analysis identifies 29 compounds in the ethanolic extract and 7 in the ethyl acetate extract. The ethanolic extract contains 3,4-Bis (Methoxycarbonyl)furan (15.76%) and 4-Imidazolidinone (14.17%), while the ethyl acetate extract is dominated by Diisooctyl phthalate (49.25%). Genoprotective activity, using the comet assay on human lymphocytes, demonstrates significant dose-dependent reductions in H<sub>2</sub>O<sub>2</sub>-induced DNA damage. The ethyl acetate extract reduces total comet score (TCS) from 265.3 ± 20.4 to 33.0 ± 4.3 at 100 mg/100 mL (p < 0.0001), outperforming the ethanolic extract, which reduced TCS from 226.7 ± 41.6 to 44.0 ± 7.2 (p < 0.001). Antioxidant activity, assessed via the DPPH method, reveals moderate activity for the ethyl acetate extract (72.61 ± 1.65%, IC<sub>50</sub>: 137.58 μg/ml) and lower activity for the ethanolic extract (67.52 ± 0.44%, IC<sub>50</sub>: 156.52 μg/ml). These findings underscore the therapeutic potential of C. radicans, particularly the ethyl acetate extract, which demonstrates genoprotective effects. Researchers should focus on isolating active compounds and exploring their potential in drug development.</p>","PeriodicalId":9831,"journal":{"name":"ChemistryOpen","volume":" ","pages":"e2500175"},"PeriodicalIF":2.5,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemistryOpenPub Date : 2025-06-05DOI: 10.1002/open.202500252
Tarad Abalkhail, Noorah A Alkubaisi, Ibrahim M Aziz, Mashail Fahad Alsayed, Hissah Abdulrahman Alodaini, Amal Saad Al-Shenifi, Mohamed A Farrag
{"title":"Evaluation of the Antioxidant, Anticancer, and Antibacterial Activities of Flower and Leaf Extracts of Chrysanthemum indicum.","authors":"Tarad Abalkhail, Noorah A Alkubaisi, Ibrahim M Aziz, Mashail Fahad Alsayed, Hissah Abdulrahman Alodaini, Amal Saad Al-Shenifi, Mohamed A Farrag","doi":"10.1002/open.202500252","DOIUrl":"https://doi.org/10.1002/open.202500252","url":null,"abstract":"<p><p>Chrysanthemum has been studied for its anti-inflammatory, antibacterial, anticancer, antioxidant, and other pharmacological properties. However, there is little knowledge about the methanol-pharmaceutical activities of Chrysanthemum indicum leaves and flowers. This study is designed to assess the in vitro antioxidant, anticancer, and antibacterial activities of C. indicum extracts. The flowers and leaves of C. indicum are extracted. Gas chromatography-mass spectrometry is used to analyze the chemical components. The antioxidant activity is evaluated using 2,2-diphenyl-1-picrilhidrazine (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. Cytotoxic effect against A549 cell line is identified. The antibacterial properties are evaluated against both Gram-positive and Gram-negative bacteria. Antioxidant activity is detected and observed to be maximum at higher concentrations. The IC50 values of flowers and leaves are 77.19 ± 2.4 and 101.94 ± 2.34 μg mL<sup>-1</sup>, respectively, in the DPPH method, whereas the ABTS assay shows 93.21 ± 3.42 and 98.22 ± 3.34 μg mL<sup>-1</sup>, respectively. The flower extract has more cytotoxic activity than the leaves (P < 0.05). The IC50 values for flowers and leaves are 72.49 ± 3.14 and 102.54 ± 4.17 μg mL<sup>-1</sup>, respectively. Furthermore, the flower and leaf extracts exhibit the most pronounced antibacterial activity. These results provide a strong basis for further research into its potential therapeutic uses as well as opportunities for the creation of natural pharmaceutical products.</p>","PeriodicalId":9831,"journal":{"name":"ChemistryOpen","volume":" ","pages":"e2500252"},"PeriodicalIF":2.5,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Porous CeO<sub>2</sub>/CuO Heterostructure for Efficient Hydrogen Evolution Reaction in an Acidic Medium.","authors":"Binod Raj Kc, Samira Munkaila, Bishnu Prasad Bastakoti","doi":"10.1002/open.202500115","DOIUrl":"https://doi.org/10.1002/open.202500115","url":null,"abstract":"<p><p>A composite electrocatalyst CeO<sub>2</sub>/CuO is fabricated for the hydrogen evolution reaction (HER) via a simple, facile, one-step hydrothermal method, using F-127 as a template and structure directing agent. The prepared composite CeO<sub>2</sub>/CuO shows top-notch HER activities with a small overpotential of 98 mV at 10 mA cm<sup>-2</sup> and just 160 mV overpotential to attain a current density of 50 mA cm<sup>-2</sup> with good stability for 20 h in an acidic medium. The enhanced catalytic activities of CeO<sub>2</sub>/CuO nanocomposite are attributed to their synergetic interface interaction, which lead to improved conductivity, reactive sites, and oxygen vacancies in their lattices. This study aims to advance the development of earth-abundant transition metal oxides-based electrocatalysts as economical, durable, and efficient HER electrocatalysts to replace noble metal-based materials in the future.</p>","PeriodicalId":9831,"journal":{"name":"ChemistryOpen","volume":" ","pages":"e2500115"},"PeriodicalIF":2.5,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemistryOpenPub Date : 2025-06-05DOI: 10.1002/open.202500223
Martina Dragone, Gianluca D'Abrosca, Antonia D'Aniello, Domenico Alberga, Getasew Shitaye, Rinaldo Grazioso, Stefano Tomassi, Luigi Russo, Roberto Fattorusso, Salvatore Di Maro, Giuseppe F Mangiatordi, Michele Saviano, Gaetano Malgieri, Carla Isernia, Rosa Iacovino
{"title":"β-Cyclodextrin Inclusion Complexes with Model Pentapeptides: Role of the Tyrosine Position within the Peptide Chain.","authors":"Martina Dragone, Gianluca D'Abrosca, Antonia D'Aniello, Domenico Alberga, Getasew Shitaye, Rinaldo Grazioso, Stefano Tomassi, Luigi Russo, Roberto Fattorusso, Salvatore Di Maro, Giuseppe F Mangiatordi, Michele Saviano, Gaetano Malgieri, Carla Isernia, Rosa Iacovino","doi":"10.1002/open.202500223","DOIUrl":"https://doi.org/10.1002/open.202500223","url":null,"abstract":"<p><p>Peptide's applications frequently present problems of solubility, stability, activity, or membrane permeability. To overcome these issues, cyclodextrins (CDs) can be used to form inclusion complexes with peptide hydrophobic parts; alkyl-chains or aromatic-rings inclusion strongly influences the interacting peptide properties. The study of model tripeptides has revealed that, among the three aromatic amino acids, tyrosine is the best suited to be included within CDs. The interaction with β-CD of five model peptides (Tyr1-5), each constituted by one tyrosine and four alanines, is reported: the tyrosine occupies one of the five position within each peptide chain. Among natural CDs, β-CD has been chosen as it is the most economic, used, and only moderately toxic; its cavity size is the best suited to accommodate the tyrosine ring. Stoichiometry and affinity of each complex are evaluated and in silico and experimental data to describe the molecular determinants of each interaction are combined. The data further defines the role of the aromatic ring position in dictating the stability of formed complexes and demonstrates Tyr3, with its central Tyr, as the most stable complex. Noteworthy, the interaction with β-CD induces Tyr3 to assume a U-shaped conformation representing a nice example of conformation stabilization upon formation of inclusion complexes.</p>","PeriodicalId":9831,"journal":{"name":"ChemistryOpen","volume":" ","pages":"e2500223"},"PeriodicalIF":2.5,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemistryOpenPub Date : 2025-06-04DOI: 10.1002/open.202580601
Panagiota M. Kalligosfyri, Antonella Miglione, Alessia Esposito, Raghad Alhardan, Gabriella Iula, Iclal Atay, Ibrahim A. Darwish, Sevinc Kurbanoglu, Stefano Cinti
{"title":"Front Cover: Flexible Screen-Printed Electrochemical Sensor for Alkaline Phosphatase Detection in Biofluids for Biomedical Applications (ChemistryOpen 6/2025)","authors":"Panagiota M. Kalligosfyri, Antonella Miglione, Alessia Esposito, Raghad Alhardan, Gabriella Iula, Iclal Atay, Ibrahim A. Darwish, Sevinc Kurbanoglu, Stefano Cinti","doi":"10.1002/open.202580601","DOIUrl":"https://doi.org/10.1002/open.202580601","url":null,"abstract":"<p><b>A flexible printed electrochemical</b> sensor capable of monitoring the activity of the alkaline phosphatase enzyme in human serum at the point of care is presented. It opens wide possibilities in clinical diagnostics; in fact, the under/over expression of this enzyme might be correlated to different diseases, from cardiovascular to cancer. The sensing system can be easily coupled to a smartphone for the readout, making the approach even more user friendly and decentralized. More details can be found in the Research Article by Panagiota M. Kalligosfyri, Sevinc Kurbanoglu, Stefano Cinti, and co-workers (DOI: 10.1002/open.202500113).\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":9831,"journal":{"name":"ChemistryOpen","volume":"14 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/open.202580601","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}