CCS Chemistry最新文献

{"title":"A Simple Synthesis of Tetraamino-[60]Fullerene Epoxides as Potential Antitumor Drug Candidates","authors":"Yicheng Lu, Jie Li, Xiaolong Liu, Dan He, Libin Yang, Wei Zhou, Xie Wang, Shanshan Chen, Shiliang Chen, Yating Liu, Xin Wang, Jiao Li, Jiawei Huo, Yang Liu, Zehu Wang, Meihan Liu, Yijun Wang, Yamin Li, Fuwen Zhao, Shumu Li, Jinchao Wei, Jianan Liu, Wei Li, Chunru Wang","doi":"10.31635/ccschem.024.202303784","DOIUrl":"https://doi.org/10.31635/ccschem.024.202303784","url":null,"abstract":"<p>Amphiphilic aminated fullerenes have a broad margin of safety and significant antitumor effects. Herein, we develop a simple and versatile synthesis strategy for tetraamino-[60]fullerene epoxide (C₆₀(NR¹R²)₄O) using C₆₀Cl₆ as a precursor, which notably reduces the reaction time to less than 1 h while retaining a high yield of over 80% with both cyclic and linear secondary amine substrates even at the gram level. The molecular structure of C₆₀(NR¹R²)₄O is first validated by single-crystal diffraction, and a two-step reaction mechanism comprising nucleophilic substitution of Cl and the oxidative elimination of Cl₂ is proposed based on experimental verification and density functional theory simulation. A set of water-soluble aminated C₆₀(NR¹R²)₄O was prepared in large quantities, and <i>in vitro</i> antitumor evaluation unveiled the critical role that terminal primary amino moieties of C₆₀(NR¹R²)₄O play in their antineoplastic effects. This work provides an effective synthesis method for aminated C₆₀(NR¹R²)₄O, facilitating the development of fullerene-derived tumor-targeted drugs.</p>","PeriodicalId":9810,"journal":{"name":"CCS Chemistry","volume":null,"pages":null},"PeriodicalIF":11.2,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140544698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Ultrastable, Easily Scalable and Regenerable Macrocycle-Based Hydrogen-Bonded Organic Framework","authors":"Zhi-Wei Li, Zi-Jun Huang, Ying-Xian Li, Xiaomei Wu, Wen Shi, Yue-Biao Zhang, Xiaomin Ma, Gangfeng Ouyang, Bao-Hui Ye, Gao-Feng Liu, Xiao-Ming Chen","doi":"10.31635/ccschem.024.202404150","DOIUrl":"https://doi.org/10.31635/ccschem.024.202404150","url":null,"abstract":"Crystalline porous materials are increasingly significant in synthetic and materials chemistry. Nonetheless, their broad industrial deployment is hampered by challenges in stability, production cost, scalability, and regenerability. Herein, we introduce a one-pot synthetic methodology for fabricating macrocycle-based hydrogen-bonded organic frameworks utilizing commercially available materials. Notably, <b xmlns:bkstg=\"http://www.atypon.com/backstage-ns\" xmlns:fn=\"http://www.w3.org/2005/xpath-functions\" xmlns:pxje=\"java:com.atypon.frontend.services.impl.PassportXslJavaExtentions\" xmlns:urlutil=\"java:com.atypon.literatum.customization.UrlUtil\" xmlns:xlink=\"http://www.w3.org/1999/xlink\">\u0000<bold>mHOF-SYSU101</bold></b>, as a distinguished exemplar, can be synthesized on a multigram scale with near-quantitative yield from raw materials of merely 70% purity, underscoring its substantial cost-efficiency. <b xmlns:bkstg=\"http://www.atypon.com/backstage-ns\" xmlns:fn=\"http://www.w3.org/2005/xpath-functions\" xmlns:pxje=\"java:com.atypon.frontend.services.impl.PassportXslJavaExtentions\" xmlns:urlutil=\"java:com.atypon.literatum.customization.UrlUtil\" xmlns:xlink=\"http://www.w3.org/1999/xlink\">\u0000<bold>mHOF-SYSU101</bold></b> demonstrates extraordinary thermal stability up to 400 °C, and exhibits remarkable chemical resilience under complex and harsh conditions over a week. This sustained stability is attributed to the strategic integration of hydrophobic methyl groups that insulate hydrogen bonds from polar molecules, coupled with multiple non-covalent interactions within its architecture. Leveraging its intrinsic one-dimensional hydrophobic channels and hydrophilic surfaces, <b xmlns:bkstg=\"http://www.atypon.com/backstage-ns\" xmlns:fn=\"http://www.w3.org/2005/xpath-functions\" xmlns:pxje=\"java:com.atypon.frontend.services.impl.PassportXslJavaExtentions\" xmlns:urlutil=\"java:com.atypon.literatum.customization.UrlUtil\" xmlns:xlink=\"http://www.w3.org/1999/xlink\">\u0000<bold>mHOF-SYSU101</bold></b> achieves a remarkable 99% adsorption of iodine from seawater in just 2 minutes and maintains this fully reversible adsorption capacity over five cycles, showing great practical utility for the nuclear power industry. Moreover, <b xmlns:bkstg=\"http://www.atypon.com/backstage-ns\" xmlns:fn=\"http://www.w3.org/2005/xpath-functions\" xmlns:pxje=\"java:com.atypon.frontend.services.impl.PassportXslJavaExtentions\" xmlns:urlutil=\"java:com.atypon.literatum.customization.UrlUtil\" xmlns:xlink=\"http://www.w3.org/1999/xlink\">\u0000<bold>mHOF-SYSU101</bold></b> can be regenerated by introducing its trifluoroacetic acid solution into dimethyl sulfoxide or methanol, endowing <b xmlns:bkstg=\"http://www.atypon.com/backstage-ns\" xmlns:fn=\"http://www.w3.org/2005/xpath-functions\" xmlns:pxje=\"java:com.atypon.frontend.services.impl.PassportXslJavaExtentions\" xmlns:urlutil=\"java:com.atypon.literatum.customization.UrlUtil\" xmlns:xlink=\"http://www.w3.org/1999/xlink\">\u0000<bold>mHOF-SYSU101</bold></b> with unprecedent","PeriodicalId":9810,"journal":{"name":"CCS Chemistry","volume":null,"pages":null},"PeriodicalIF":11.2,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140348931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Author Spotlight","authors":"","doi":"10.31635/ccschem.024.202400320ed2","DOIUrl":"https://doi.org/10.31635/ccschem.024.202400320ed2","url":null,"abstract":"CCS Chemistry, Volume 6, Issue 4, Page 829-832, April 2024.<br/>","PeriodicalId":9810,"journal":{"name":"CCS Chemistry","volume":null,"pages":null},"PeriodicalIF":11.2,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140346320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Strategies of Tailoring PEDOT:PSS for Bioelectronic Applications: Synthesis, Processing and Device Fabrication","authors":"Yewei Huang, Lingfeng Tang, Yuanwen Jiang","doi":"10.31635/ccschem.024.202403858","DOIUrl":"https://doi.org/10.31635/ccschem.024.202403858","url":null,"abstract":"Poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) has been the most prominent conducting polymer due to its outstanding electrical properties, chemical stability, biocompatibility, and commercial availability. In this mini review, we aim to comprehensively outline the chemical approaches employed in tailoring PEDOT:PSS for bioelectronic applications. We open our discussion by showcasing various synthetic techniques and commercially accessible forms of PEDOT:PSS, providing practical advice and approaches to greatly enhance its electrical properties, and presenting diverse chemical designs and processing methods that are essential for converting PEDOT:PSS into different form factors, such as fibers, gels, and films, for integration a range of device structures. Additionally, we explore several burgeoning applications of PEDOT:PSS in bioelectronics, ranging from wearable health monitoring to implantable neural interfaces, underscoring its essential impact on improving device efficiency and biological compatibility, as it opens avenues for innovative diagnostic and therapeutic techniques in the realm of precision medicine. Concluding with an outlook, the review presents insights into the ongoing challenges and future research paths for PEDOT:PSS in the ever-evolving landscape of bioelectronics. We emphasize the need for continued innovation in materials science and engineering to further harness the full potential of this dynamic domain.\u0000<figure><img alt=\"\" data-lg-src=\"/cms/asset/dab243bd-8367-4995-9cf3-2f3c580b84c9/keyimage.jpg\" data-src=\"/cms/asset/b56d1495-15e7-4933-a1cd-e3ab5311dd74/keyimage.jpg\" src=\"/specs/ux3/releasedAssets/images/loader-7e60691fbe777356dc81ff6d223a82a6.gif\"/><ul>\u0000<li>Download figure</li>\u0000<li>Download PowerPoint</li>\u0000</ul>\u0000</figure>","PeriodicalId":9810,"journal":{"name":"CCS Chemistry","volume":null,"pages":null},"PeriodicalIF":11.2,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140343463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetraazahexacene Diimides with Ultralow LUMO Levels","authors":"Shuhai Qiu, Li Zhang, Xin Jin, Chengxi Zhao, Junzhi Liu, Da-Hui Qu, Wei Jiang, Zhaohui Wang","doi":"10.31635/ccschem.024.202404126","DOIUrl":"https://doi.org/10.31635/ccschem.024.202404126","url":null,"abstract":"Azaacene diimides are expected to possess deep LUMO levels and narrower band gaps while maintaining excellent solubility and enhanced stability compared to their parent acenes. However, the synthesis of fully conjugated azaacenes has posed a long-term challenge. In this study, we report a stepwise oxidation approach to successfully synthesize a series of fully conjugated tetraazahexacene diimides (<b xmlns:bkstg=\"http://www.atypon.com/backstage-ns\" xmlns:fn=\"http://www.w3.org/2005/xpath-functions\" xmlns:pxje=\"java:com.atypon.frontend.services.impl.PassportXslJavaExtentions\" xmlns:urlutil=\"java:com.atypon.literatum.customization.UrlUtil\" xmlns:xlink=\"http://www.w3.org/1999/xlink\">\u0000<bold>TAHDIs</bold></b>). Through periphery engineering, the targeted <b xmlns:bkstg=\"http://www.atypon.com/backstage-ns\" xmlns:fn=\"http://www.w3.org/2005/xpath-functions\" xmlns:pxje=\"java:com.atypon.frontend.services.impl.PassportXslJavaExtentions\" xmlns:urlutil=\"java:com.atypon.literatum.customization.UrlUtil\" xmlns:xlink=\"http://www.w3.org/1999/xlink\">\u0000<bold>TAHDIs</bold></b>, featuring electron-donating ethylenedioxy rings, exhibited significantly enhanced stability, with a half-life time of up to 65 h under ambient conditions. Furthermore, single-crystal structural elucidation revealed the nearly planar geometry, with imide rings slightly deviating from the skeleton plane. Notably, the LUMO levels were determined to be as low as -4.68 eV for <b xmlns:bkstg=\"http://www.atypon.com/backstage-ns\" xmlns:fn=\"http://www.w3.org/2005/xpath-functions\" xmlns:pxje=\"java:com.atypon.frontend.services.impl.PassportXslJavaExtentions\" xmlns:urlutil=\"java:com.atypon.literatum.customization.UrlUtil\" xmlns:xlink=\"http://www.w3.org/1999/xlink\">\u0000<bold>TAHDI-<i>ad</i></bold></b> and could be noticeably elevated to -4.53 eV through the attachment of electron-donating groups (<b xmlns:bkstg=\"http://www.atypon.com/backstage-ns\" xmlns:fn=\"http://www.w3.org/2005/xpath-functions\" xmlns:pxje=\"java:com.atypon.frontend.services.impl.PassportXslJavaExtentions\" xmlns:urlutil=\"java:com.atypon.literatum.customization.UrlUtil\" xmlns:xlink=\"http://www.w3.org/1999/xlink\">\u0000<bold>TAHDI-<i>ce</i></bold></b>). This work represents a rare example of an experimetally evidenced low-lying LUMO level, providing an alternative strategy for developing higher fully conjugated azacene diimides which have great promise in electronics and molecular electronics.\u0000<figure><img alt=\"\" data-lg-src=\"/cms/asset/32c34bb3-8f6e-4ded-afd7-f937100eed74/keyimage.jpg\" data-src=\"/cms/asset/d758ba1b-92d5-4045-9a97-f39bb70d7fcb/keyimage.jpg\" src=\"/specs/ux3/releasedAssets/images/loader-7e60691fbe777356dc81ff6d223a82a6.gif\"/><ul>\u0000<li>Download figure</li>\u0000<li>Download PowerPoint</li>\u0000</ul>\u0000</figure>","PeriodicalId":9810,"journal":{"name":"CCS Chemistry","volume":null,"pages":null},"PeriodicalIF":11.2,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140343535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cove-Edged Boron-Doped peri-Acenes via Alkyne-Enabled Cyclization","authors":"Zengming Fan, Yujia Liu, Tianyu Zhang, Yue Wang, Chuandong Dou","doi":"10.31635/ccschem.024.202403953","DOIUrl":"https://doi.org/10.31635/ccschem.024.202403953","url":null,"abstract":"Heteroatom-doping of polycyclic aromatic hydrocarbons may alter their electronic structures and thereby achieve intriguing physical properties. However, it is very challenging to dope the boron atom into [n,m]<i>peri</i>-acenes due to the synthetic difficulty and limited synthetic method. Herein, we disclose implementation of alkyne-enabled cyclization on organoborane π-system to construct cove-edged boron-doped [n,m]<i>peri</i>-acenes (BPAs). We synthesized two boron-containing π-systems that own the C₄₄B₂ and C₆₂B₂ polycyclic conjugated skeletons, respectively. Both of them feature two boron atoms and two cove regions at the zigzag edges, and thus may be regarded as an unprecedented kind of cove-edged BPAs. Detailed studies illustrate that these cove-edged BPAs exhibit significantly modulated electronic structures and properties, such as distinctive global aromaticity, multi-reversible redox activity and tunable photophysical properties. Notably, they exhibit the obviously stabilized molecular orbitals, especially HOMOs, further leading to the enlarged energy gaps and excellent ambient stability. Moreover, the stimulated emission (SE) and amplified spontaneous emission (ASE) properties are achieved for the C₄₄B₂ molecule, thus not only representing a new example of ASE-active organic materials but also demonstrating the promising utility in organic photonics. As revealed, the boron-doping and cove-edging both play a prominent role in producing these remarkable electronic effects and properties.\u0000<figure><img alt=\"\" data-lg-src=\"/cms/asset/475d2b66-7e7c-46b2-b08c-36ebcb1fed8f/keyimage.jpg\" data-src=\"/cms/asset/ee69ab9d-8a1d-4ba7-bc30-d93d14ab880e/keyimage.jpg\" src=\"/specs/ux3/releasedAssets/images/loader-7e60691fbe777356dc81ff6d223a82a6.gif\"/><ul>\u0000<li>Download figure</li>\u0000<li>Download PowerPoint</li>\u0000</ul>\u0000</figure>","PeriodicalId":9810,"journal":{"name":"CCS Chemistry","volume":null,"pages":null},"PeriodicalIF":11.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140343471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoelectrochemical Iron-Catalyzed C(sp3)–H Borylation of Alkanes in a Position-Selective Manner","authors":"Wen Wei, Bin Wang, Simon L. Homölle, Jinbin Zhu, Yanjun Li, Tristan von Münchow, Isaac Maksso, Lutz Ackermann","doi":"10.31635/ccschem.024.202403894","DOIUrl":"https://doi.org/10.31635/ccschem.024.202403894","url":null,"abstract":"<p>Catalytic C–H borylation is of prime topical importance, since easily available feedstock chemicals can thereby be transformed into valuable transient functional groups in the absence of directing groups. Herein, we disclose an iron-catalyzed C(sp³)–H borylation of simple, non-prefunctionalized alkanes, providing access to value-added products in a single step by means of photoelectrochemistry. The power of merging photo- and electrochemistry was mirrored by ample scope and exceedingly mild reaction conditions. Moreover, an outstanding position-selectivity in favor of primary C(sp³)–H proved viable within the photoelectrochemical borylation through a chemo-selective anodic overoxidation manifold. The ferro-photoelectrochemistry strategy avoids toxic precious transition metals, enabling C(sp³)–H borylations in a site-selective fashion.</p>","PeriodicalId":9810,"journal":{"name":"CCS Chemistry","volume":null,"pages":null},"PeriodicalIF":11.2,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140321955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial Chlorophyll-like Structure for Photocatalytic CO2 Chemical Fixation","authors":"Lei Li, Wenxiu Liu, Hanghao Ying, Xin He, Shu Shang, Peng Zhang, Xiaodong Zhang, Shuhu Liu, Hui Wang, Yi Xie","doi":"10.31635/ccschem.024.202404189","DOIUrl":"https://doi.org/10.31635/ccschem.024.202404189","url":null,"abstract":"In nature, green leaves accomplish photosynthesis via chloroplasts, where the Mg-N₄ centered chlorophyll and enzymes undertake light absorption and catalytic reaction, respectively. This intriguing phenomenon inspires us to design an artificial photosynthetic system that synergizes these dual attributes, a field that has remained largely unexplored. Herein, we develop a highly stable artificial chlorophyll-like nitrogen-decorated amorphous carbon material (Mg-NC) featuring abundant Mg-N₄ moieties (with high Mg content reaching ≈ 9.2 wt%), which simultaneously integrates the light-absorption center and reaction center to mimic natural photosynthesis of CO₂ valorization. As demonstrated, taking photocatalytic CO₂ cycloaddition reaction as an example, the Mg-N₄ active center in Mg-NC plays as an electronic donor, which can readily inject hot electrons to the adsorbed substrates, resulting in the swift formation of cyclic carbonate products. Remarkably, the as-designed Mg-NC exhibited outstanding photocatalytic CO₂ cycloaddition performance, achieving an impressive reaction rate up to 9.67 mmol·g^-1·h^-1 under mild conditions, which could be kept even under gram scale conditions. Our study offers an artificial biomimetic enzyme heterogeneous photocatalysts for CO₂ valorization.\u0000<figure><img alt=\"\" data-lg-src=\"/cms/asset/0820ecad-cc34-469b-860f-d8f2eafdc96c/keyimage.jpg\" data-src=\"/cms/asset/303ad7b6-01cc-45f5-949f-5b83e97bc94d/keyimage.jpg\" src=\"/specs/ux3/releasedAssets/images/loader-7e60691fbe777356dc81ff6d223a82a6.gif\"/><ul>\u0000<li>Download figure</li>\u0000<li>Download PowerPoint</li>\u0000</ul>\u0000</figure>","PeriodicalId":9810,"journal":{"name":"CCS Chemistry","volume":null,"pages":null},"PeriodicalIF":11.2,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140322020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphine-Catalyzed Asymmetric Dearomative [3+2] Annulation Reaction of Benzimidazoles with Cyclopropenones","authors":"Shan-Shan Zhang, Rui-Xiang Wang, Qing Gu, Shu-Li You","doi":"10.31635/ccschem.024.202403981","DOIUrl":"https://doi.org/10.31635/ccschem.024.202403981","url":null,"abstract":"A phosphine-catalyzed intermolecular asymmetric dearomatization reaction of benzimidazoles with cyclopropenones has been developed. In the presence of 5 mol% of commercially available chiral phosphine catalyst, the dearomative [3+2] annulation reaction proceeds smoothly to afford the corresponding dearomatized heterocycles in excellent yields (up to 98%) and enantioselectivity (up to 99% ee). Meanwhile, this reaction features mild reaction conditions, excellent functional group tolerance, and the compatibility with gram-scale synthesis.\u0000<figure><img alt=\"\" data-lg-src=\"/cms/asset/9b358c8c-9b58-4d0e-8607-0d68e6825e91/keyimage.jpg\" data-src=\"/cms/asset/8c327161-9011-403d-8fed-03672e952168/keyimage.jpg\" src=\"/specs/ux3/releasedAssets/images/loader-7e60691fbe777356dc81ff6d223a82a6.gif\"/><ul>\u0000<li>Download figure</li>\u0000<li>Download PowerPoint</li>\u0000</ul>\u0000</figure>","PeriodicalId":9810,"journal":{"name":"CCS Chemistry","volume":null,"pages":null},"PeriodicalIF":11.2,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140340620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactional analysis of single mitochondrial function directly in living cell reveals the proton circuit decoupling of mitochondria at the preliminary stage of apoptosis","authors":"Baoguo Wang, Jin Zhu, Tianxiang Wei, Yixin Zhang, Mingyue Li, Zilong Zhang, Tao Gao, Zhihui Dai","doi":"10.31635/ccschem.024.202404066","DOIUrl":"https://doi.org/10.31635/ccschem.024.202404066","url":null,"abstract":"Analyzing single organelle within the intricate compartmentalized systems of eukaryotic cells poses significant challenges in biomedical research. Presently, assessment of the organelle functions fre-quently relies on the disjointed application of multiple techniques, resulting in constraints on the promptness and precision of data acquisition. Herein, a dual-nanopore electrochemical biosensor has been designed in this work to house detection channels directly in living cell for <i>in situ</i> and concurrent acquisition of multiple key characteristics of a single mitochondrion. In one channel, mitochondrial ROS and associated ATP production are electrochemically measured with a redox acti-vatable probe (Apt-MB), while the parallel channel facilitates the measurement of mitochondrial ΔΨm <i>via</i> proton responsiveness. Quantitative assessment of the rela-tionship between ROS and ΔΨm against ATP production have been achieved, which allows in-depth analysis of mitochondrial functions. With this approach, the results clearly show evolu-tionary stages of the apoptosis pathway, and highlight that the mitochondrial proton circuit decoupling may serve as an earlier event preceding the well-known ROS accumulation stage. Hence, this study underscores the considerable usage of this approach for comprehensive analysis of single mitochondri-al function, and may further inspire the development of micro/nanoscale tools for multi-parameter, high-fidelity, single-organelle analysis directly within cell.\u0000<figure><img alt=\"\" data-lg-src=\"/cms/asset/6d8dd7a2-5485-499f-9e79-0878c4cfb095/keyimage.jpg\" data-src=\"/cms/asset/de360d92-a622-4392-b29e-183655ec53e1/keyimage.jpg\" src=\"/specs/ux3/releasedAssets/images/loader-7e60691fbe777356dc81ff6d223a82a6.gif\"/><ul>\u0000<li>Download figure</li>\u0000<li>Download PowerPoint</li>\u0000</ul>\u0000</figure>","PeriodicalId":9810,"journal":{"name":"CCS Chemistry","volume":null,"pages":null},"PeriodicalIF":11.2,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140340710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}