Cell and Tissue BankingPub Date : 2024-06-01Epub Date: 2024-02-21DOI: 10.1007/s10561-024-10128-1
Jiaxin Liu, Yang Yang, Yun Qi
{"title":"Efficacy of mesenchymal stromal cells in the treatment of type 1 diabetes: a systematic review.","authors":"Jiaxin Liu, Yang Yang, Yun Qi","doi":"10.1007/s10561-024-10128-1","DOIUrl":"10.1007/s10561-024-10128-1","url":null,"abstract":"<p><p>To investigate the efficacy of mesenchymal stromal cells in the treatment of type 1 diabetes. Articles about the effects of mesenchymal stromal cells for T1D were retrieved in PubMed, Web of Science, Embase, and the Cochrane Library databases up to July 2023. Additional relevant studies were manually searched through citations. HbA1c, FBG, PBG, insulin requirement and C-peptide were assessed. The risk of bias was evaluated with the ROB 2.0 and ROBINS-I tools. Six RCTs and eight nRCTs were included. Of the 14 studies included, two evaluated BM-MSCs, three evaluated UC-MSCs, five evaluated AHSCT, two evaluated CB-SCs, and two evaluated UC-SCs plus aBM-MNCs. At the end of follow-up, ten studies found that mesenchymal stromal cells improved glycemic outcomes in T1D, while the remaining four studies showed no significant improvement. Findings support the positive impacts observed from utilizing mesenchymal stromal cells in individuals with T1D. However, the overall methodological quality of the identified studies and findings is heterogeneous, limiting the interpretation of the therapeutic benefits of mesenchymal stromal cells in T1D. Methodically rigorous research is needed to further increase credibility.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"663-676"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11143029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139930240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cell and Tissue BankingPub Date : 2024-06-01Epub Date: 2024-01-03DOI: 10.1007/s10561-023-10122-z
Liane Marise Röhsig, Nance Beyer Nardi
{"title":"Impact of COVID-19 pandemic on cord blood banking and transplantation.","authors":"Liane Marise Röhsig, Nance Beyer Nardi","doi":"10.1007/s10561-023-10122-z","DOIUrl":"10.1007/s10561-023-10122-z","url":null,"abstract":"<p><p>Umbilical cord blood is a rich source of hematopoietic stem cells that has been used for transplantation for over 30 years, especially when there is no compatible hematopoietic stem cell donor available. Its use has decreased more recently, since the development of methods to improve haploidentical transplants has allowed the use of mobilized peripheral blood as a source of hematopoietic stem cells. Public cord blood banks collect, process and store cord blood samples from voluntary donations. In addition, many public banks are involved in research to enhance hematopoietic stem cell therapies and develop new treatments for haematological and genetic diseases. The COVID-19 pandemic, which emerged in 2019, has had a profound and wide-ranging impact on human health and treatment. The area of hematopoietic stem cell transplantation was deeply affected by reductions in bone marrow, peripheral blood and cord blood donations; logistical challenges; exposure of healthcare workers and other challenges. The present study reviews the impact of the COVID-19 pandemic on cord blood banking and transportation around the world with a special focus on Brazil.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"605-611"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139086049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Decellularized kidney capsule as a three-dimensional scaffold for tissue regeneration","authors":"Mohammad Rasool Khazaei, Rawa Ibrahim, Rayan Faris, Azam Bozorgi, Mozafar Khazaei, Leila Rezakhani","doi":"10.1007/s10561-024-10136-1","DOIUrl":"https://doi.org/10.1007/s10561-024-10136-1","url":null,"abstract":"<p>Tissue regeneration is thought to have considerable promise with the use of scaffolds designed for tissue engineering. Although polymer-based scaffolds for tissue engineering have been used extensively and developed quickly, their ability to mimic the in-vivo milieu, overcome immunogenicity, and have comparable mechanical or biochemical properties has limited their capability for repair. Fortunately, there is a compelling method to get around these challenges thanks to the development of extracellular matrix (ECM) scaffolds made from decellularized tissues. We used ECM decellularized sheep kidney capsule tissue in our research. Using detergents such as Triton-X100 and sodium dodecyl sulfate (SDS), these scaffolds were decellularized. DNA content, histology, mechanical properties analysis, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), biocompatibility, hemocompatibility and scanning electron microscope (SEM) imaging were measured. The results showed that the three-dimensional (3D) structure of the ECM remained largely intact. The scaffolds mentioned above had several hydrophilic properties. The best biocompatibility and blood compatibility properties were reported in the SDS method of 0.5%. The best decellularization scaffold was introduced with 0.5% SDS. Therefore, it can be proposed as a scaffold that has ECM like natural tissue, for tissue engineering applications.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"21 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Recent progress in mesenchymal stem cell-based therapy for acute lung injury","authors":"Jinfeng Liang, Weiyou Dai, Shihang Xue, Feifei Wu, Enhai Cui, Ruolang Pan","doi":"10.1007/s10561-024-10129-0","DOIUrl":"https://doi.org/10.1007/s10561-024-10129-0","url":null,"abstract":"<p>Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are life-threatening diseases in critically ill patients. Although pathophysiology of ALI/ARDS has been investigated in many studies, effective therapeutic strategies are still limited. Mesenchymal stem cell (MSC)-based therapy is emerging as a promising therapeutic intervention for patients with ALI. During the last two decades, researchers have focused on the efficacy and mechanism of MSC application in ALI animal models. MSC derived from variant resources exhibited therapeutic effects in preclinical studies of ALI with different mechanisms. Based on this, clinical studies on MSC treatment in ALI/ARDS has been tried recently, especially in COVID-19 caused lung injury. Emerging clinical trials of MSCs in treating COVID-19-related conditions have been registered in past two years. The advantages and potential of MSCs in the defense against COVID-19-related ALI or ARDS have been confirmed. This review provides a brief overview of recent research progress in MSC-based therapies in preclinical study and clinical trials in ALI treatment, as well as the underlying mechanisms.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"35 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140099339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cell and Tissue BankingPub Date : 2024-03-01Epub Date: 2023-05-05DOI: 10.1007/s10561-023-10091-3
Carlos Alexandre Curylofo Corsi, Claudia Tarcila Gomes Sares, Fabiola Mestriner, Jéssyca Michelon-Barbosa, Vinicius Flora Dugaich, Timna Varela Martins, Alex Martins Násare, Roberta Ribeiro Costa Rosales, Maria Cecília Jordani, José Carlos Alves-Filho, Rodolfo Borges Dos Reis, Mauricio Serra Ribeiro, Christiane Becari
{"title":"Isolation and primary culture of human abdominal aorta smooth muscle cells from brain-dead donors: an experimental model for vascular diseases.","authors":"Carlos Alexandre Curylofo Corsi, Claudia Tarcila Gomes Sares, Fabiola Mestriner, Jéssyca Michelon-Barbosa, Vinicius Flora Dugaich, Timna Varela Martins, Alex Martins Násare, Roberta Ribeiro Costa Rosales, Maria Cecília Jordani, José Carlos Alves-Filho, Rodolfo Borges Dos Reis, Mauricio Serra Ribeiro, Christiane Becari","doi":"10.1007/s10561-023-10091-3","DOIUrl":"10.1007/s10561-023-10091-3","url":null,"abstract":"<p><p>Primary cell cultures are essential tools for elucidating the physiopathological mechanisms of the cardiovascular system. Therefore, a primary culture growth protocol of cardiovascular smooth muscle cells (VSMCs) obtained from human abdominal aortas was standardized. Ten abdominal aorta samples were obtained from patients diagnosed with brain death who were organ and tissue donors with family consent. After surgical ablation to capture the aorta, the aortic tissue was removed, immersed in a Custodiol® solution, and kept between 2 and 8 °C. In the laboratory, in a sterile environment, the tissue was fragmented and incubated in culture plates containing an enriched culture medium (DMEM/G/10% fetal bovine serum, L-glutamine, antibiotics and antifungals) and kept in an oven at 37 °C and 5% CO<sub>2</sub>. The aorta was removed after 24 h of incubation, and the culture medium was changed every six days for twenty days. Cell growth was confirmed through morphological analysis using an inverted optical microscope (Nikon®) and immunofluorescence for smooth muscle alpha-actin and nuclei. The development of the VSMCs was observed, and from the twelfth day, differentiation, long cytoplasmic projections, and adjacent cell connections occurred. On the twentieth day, the morphology of the VSMCs was confirmed by actin fiber immunofluorescence, which is a typical characteristic of VSMCs. The standardization allowed VSMC growth and the replicability of the in vitro test, providing a protocol that mimics natural physiological environments for a better understanding of the cardiovascular system. Its use is intended for investigation, tissue bioengineering, and pharmacological treatments.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"187-194"},"PeriodicalIF":1.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9403180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of the effects of preservation methods on structural, biological, and mechanical properties of the human amniotic membrane for medical applications.","authors":"Ameneh Jafari, Yousef Mirzaei, Ali Hussein Mer, Mostafa Rezaei-Tavirani, Zahra Jafari, Hassan Niknejad","doi":"10.1007/s10561-023-10114-z","DOIUrl":"10.1007/s10561-023-10114-z","url":null,"abstract":"<p><p>Amniotic membrane (AM), the innermost layer of the placenta, is an exceptionally effective biomaterial with divers applications in clinical medicine. It possesses various biological functions, including scar reduction, anti-inflammatory properties, support for epithelialization, as well as anti-microbial, anti-fibrotic and angio-modulatory effects. Furthermore, its abundant availability, cost-effectiveness, and ethical acceptability make it a compelling biomaterial in the field of medicine. Given the potential unavailability of fresh tissue when needed, the preservation of AM is crucial to ensure a readily accessible and continuous supply for clinical use. However, preserving the properties of AM presents a significant challenge. Therefore, the establishment of standardized protocols for the collection and preservation of AM is vital to ensure optimal tissue quality and enhance patient safety. Various preservation methods, such as cryopreservation, lyophilization, and air-drying, have been employed over the years. However, identifying a preservation method that effectively safeguards AM properties remains an ongoing endeavor. This article aims to review and discuss different sterilization and preservation procedures for AM, as well as their impacts on its histological, physical, and biochemical characteristics.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"305-323"},"PeriodicalIF":1.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cell and Tissue BankingPub Date : 2024-03-01Epub Date: 2020-09-02DOI: 10.1007/s10561-020-09861-0
Lívia Contini Massimino, Virginia da Conceição Amaro Martins, Valcinir Aloisio Scalla Vulcani, Éverton Lucas de Oliveira, Mariane Barsi Andreeta, Tito José Bonagamba, Maria Fátima Guarizo Klingbeil, Monica Beatriz Mathor, Ana Maria de Guzzi Plepis
{"title":"Use of collagen and auricular cartilage in bioengineering: scaffolds for tissue regeneration.","authors":"Lívia Contini Massimino, Virginia da Conceição Amaro Martins, Valcinir Aloisio Scalla Vulcani, Éverton Lucas de Oliveira, Mariane Barsi Andreeta, Tito José Bonagamba, Maria Fátima Guarizo Klingbeil, Monica Beatriz Mathor, Ana Maria de Guzzi Plepis","doi":"10.1007/s10561-020-09861-0","DOIUrl":"10.1007/s10561-020-09861-0","url":null,"abstract":"<p><p>The aim of this study was the development of collagen and collagen/auricular cartilage scaffolds for application in dermal regeneration. Collagen was obtained from bovine tendon by a 72 h-long treatment, while bovine auricular cartilage was treated for 24 h and divided into two parts, external (perichondrium, E) and internal (elastic cartilage, I). The scaffolds were prepared by mixing collagen (C) with the internal part (CI) or the external part (CE) in a 3:1 ratio. Differential scanning calorimetry, scanning electron microscopy (SEM) analysis, microcomputed tomography imaging (micro-CT) and swelling degree were used to characterize the scaffolds. Cytotoxicity, cell adhesion, and cell proliferation assays were performed using the cell line NIH/3T3. All samples presented a similar denaturation temperature (Td) around 48 °C, while CE presented a second Td at 51.2 °C. SEM micrographs showed superficial pores in all scaffolds and micro-CT exhibited interconnected pore spaces with porosity above 60% (sizes between 47 and 149 µm). The order of swelling was CE < CI < C and the scaffolds did not present cytotoxicity, showing attachment rates above 75%-all samples showed a similar pattern of proliferation until 168 h, whereas CI tended to decrease after this time. The scaffolds were easily obtained, biocompatible and had adequate morphology for cell growth. All samples showed high adhesion, whereas collagen-only and collagen/external part scaffolds presented a better cell proliferation rate and would be indicated for possible use in dermal regeneration.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"111-122"},"PeriodicalIF":1.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38339700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The trend of allogeneic tendon decellularization: literature review.","authors":"Yangyang Jin, Qi Sun, Rongxing Ma, Ruifeng Li, Ruiqi Qiao, Jikai Li, Limin Wang, Yongcheng Hu","doi":"10.1007/s10561-023-10097-x","DOIUrl":"10.1007/s10561-023-10097-x","url":null,"abstract":"<p><p>Tendon injuries repair is a significant burden for orthopaedic surgeons. Finding a proper graft material to repair tendon is one of the main challenges in orthopaedics, for which the requirement of substitute for tendon repair would be different for each clinical application. Among biological scaffolds, the use of decellularized tendon increasingly represents an interesting approach to treat tendon injuries and several articles have investigated the approaches of tendon decellularization. To understand the outcomes of the the approaches of tendon decellularization on effect of tendon transplantation, a literature review was performed. This review was conducted by searching in Pubmed and Embase and 64 studies were included in this study. The findings revealed that the common approaches to decellularize tendon include chemical, physical, and enzymatic decellularization methods or their combination. With the development of tissue engineering, researchers also put forward new theories such as automatic acellular machine, 3D printing technology to manufacture acellular scaffold.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"357-367"},"PeriodicalIF":1.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9671811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cell and Tissue BankingPub Date : 2024-03-01Epub Date: 2023-03-14DOI: 10.1007/s10561-023-10082-4
Dmitry Bobylev, Alexander Horke, Murat Avsar, Tomislav Cvitkovic, Dietmar Boethig, Mark Hazekamp, Bart Meyns, Filip Rega, Hitendu Dave, Martin Schmiady, Anatol Ciubotaru, Eduard Cheptanaru, Vladimiro Vida, Massimo Padalino, Victor Tsang, Ramadan Jashari, Günther Laufer, Martin Andreas, Alexandra Andreeva, Igor Tudorache, Serghei Cebotari, Axel Haverich, Samir Sarikouch
{"title":"Matched comparison of decellularized homografts and bovine jugular vein conduits for pulmonary valve replacement in congenital heart disease.","authors":"Dmitry Bobylev, Alexander Horke, Murat Avsar, Tomislav Cvitkovic, Dietmar Boethig, Mark Hazekamp, Bart Meyns, Filip Rega, Hitendu Dave, Martin Schmiady, Anatol Ciubotaru, Eduard Cheptanaru, Vladimiro Vida, Massimo Padalino, Victor Tsang, Ramadan Jashari, Günther Laufer, Martin Andreas, Alexandra Andreeva, Igor Tudorache, Serghei Cebotari, Axel Haverich, Samir Sarikouch","doi":"10.1007/s10561-023-10082-4","DOIUrl":"10.1007/s10561-023-10082-4","url":null,"abstract":"<p><p>For decades, bovine jugular vein conduits (BJV) and classic cryopreserved homografts have been the two most widely used options for pulmonary valve replacement (PVR) in congenital heart disease. More recently, decellularized pulmonary homografts (DPH) have provided an alternative avenue for PVR. Matched comparison of patients who received DPH for PVR with patients who received bovine jugular vein conduits (BJV) considering patient age group, type of heart defect, and previous procedures. 319 DPH patients were matched to 319 BJV patients; the mean age of BJV patients was 15.3 (SD 9.5) years versus 19.1 (12.4) years in DPH patients (p = 0.001). The mean conduit diameter was 24.5 (3.5) mm for DPH and 20.3 (2.5) mm for BJV (p < 0.001). There was no difference in survival rates between the two groups after 10 years (97.0 vs. 98.1%, p = 0.45). The rate of freedom from endocarditis was significantly lower for BJV patients (87.1 vs. 96.5%, p = 0.006). Freedom from explantation was significantly lower for BJV at 10 years (81.7 vs. 95.5%, p = 0.001) as well as freedom from any significant degeneration at 10 years (39.6 vs. 65.4%, p < 0.001). 140 Patients, matched for age, heart defect type, prior procedures, and conduit sizes of 20-22 mm (± 2 mm), were compared separately; mean age BJV 8.7 (4.9) and DPH 9.5 (7.3) years (p = n.s.). DPH showed 20% higher freedom from explantation and degeneration in this subgroup (p = 0.232). Decellularized pulmonary homografts exhibit superior 10-year results to bovine jugular vein conduits in PVR.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"55-66"},"PeriodicalIF":1.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10901942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9111285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cell and Tissue BankingPub Date : 2024-03-01Epub Date: 2023-10-04DOI: 10.1007/s10561-023-10089-x
Mariusz Birbach, Maciej Fedorowicz, Ewa M Gałkowska, Agnieszka Powirska, Michał Kozłowski, Krzysztof Mozol, Aleksandra Wasiak, Bohdan Maruszewski, Andrzej Kansy
{"title":"Using cryopreserved allogeneic pericardium to repair congenital heart defects in children.","authors":"Mariusz Birbach, Maciej Fedorowicz, Ewa M Gałkowska, Agnieszka Powirska, Michał Kozłowski, Krzysztof Mozol, Aleksandra Wasiak, Bohdan Maruszewski, Andrzej Kansy","doi":"10.1007/s10561-023-10089-x","DOIUrl":"10.1007/s10561-023-10089-x","url":null,"abstract":"<p><p>Patches prepared from autologous, allogeneic, or xenogeneic tissues are widely used in the repair of congenital heart defects in children. Since 2002, cryopreserved allogeneic pericardial patches have been prepared in our institution as an alternative to commercially available patches. This study retrospectively reviewed donor and patient data concerning cryopreservation time and the clinical use of the pericardium in 382 children who were operated on at a single center between 2004 and 2021. There were 177 donors: 98 males and 79 females. The median donor age was 13 years (range: 1 month to 53 years) and the median cryopreservation time was 72 days (range: 3-685). There were 382 pediatric patients: 224 males and 158 females. The median patient age was 1 month (range: 3 days to 17.8 years). The patches were used for primary surgeries in 228 patients and for reoperations in 154. The patches were implanted into the right heart or venous circulation in 209 patients, the left heart or arterial circulation in 246 patients, and both sides of the circulatory system in 73. Extracardiac patch implantation was performed in 339 patients, intracardiac in 79 patients, and both intracardiac and extracardiac in 36 patients. Our study presents a single-center experience in the use of cryopreserved allogeneic pericardium. The pericardium can be used on the systemic and pulmonary sides of the circulatory system, in either extracardiac or intracardiac positions. However, there is no uniform strategy for selecting the \"patch of choice\" for correcting congenital heart defects in children, especially since there are few studies comparing several types of patches.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"99-109"},"PeriodicalIF":1.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10902029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41129011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}