Cell and Tissue Banking最新文献_第6页

Retraction Note: Approaches to reducing the toxic exposure hazard on the sheep population. 撤回说明：减少绵羊群体毒性暴露危害的方法。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 DOI: 10.1007/s10561-023-10113-0

Mynbay Umitzhanov, Asiya K Musaeva, Abdikalyk A Abishov, Toktar M Zhamansarin, Urzhan Zh Omarbekova, Sholpan Zh Turyspayeva, Sarsenbek T Siyabekov

引用次数: 0

Remaining microtia tissue as a source for 3D bioprinted elastic cartilage tissue constructs, potential use for surgical microtia reconstruction. 剩余的小体组织作为3D生物打印弹性软骨组织结构的来源，可能用于外科小体重建。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-12-01 DOI: 10.1007/s10561-023-10118-9

Cristina Velasquillo, Yaaziel Melgarejo-Ramírez, Julieta García-López, Claudia Gutiérrez-Gómez, Hugo Lecona, Maykel González-Torres, José Iván Sánchez-Betancourt, Clemente Ibarra, Sang Jin Lee, James J Yoo

{"title":"Remaining microtia tissue as a source for 3D bioprinted elastic cartilage tissue constructs, potential use for surgical microtia reconstruction.","authors":"Cristina Velasquillo, Yaaziel Melgarejo-Ramírez, Julieta García-López, Claudia Gutiérrez-Gómez, Hugo Lecona, Maykel González-Torres, José Iván Sánchez-Betancourt, Clemente Ibarra, Sang Jin Lee, James J Yoo","doi":"10.1007/s10561-023-10118-9","DOIUrl":"10.1007/s10561-023-10118-9","url":null,"abstract":"The absence of ears in children is a global problem. An implant made of costal cartilage is the standard procedure for ear reconstruction; however, side effects such as pneumothorax, loss of thoracic cage shape, and respiratory complications have been documented. Three-dimensional (3D) printing allows the generation of biocompatible scaffolds that mimic the shape, mechanical strength, and architecture of the native extracellular matrix necessary to promote new elastic cartilage formation. We report the potential use of a 3D-bioprinted poly-ε-caprolactone (3D-PCL) auricle-shaped framework seeded with remaining human microtia chondrocytes for the development of elastic cartilage for autologous microtia ear reconstruction. An in vivo assay of the neo-tissue formed revealed the generation of a 3D pinna-shaped neo-tissue, and confirmed the formation of elastic cartilage by the presence of type II collagen and elastin with histological features and a protein composition consistent with normal elastic cartilage. According to our results, a combination of 3D-PCL auricle frameworks and autologous microtia remnant tissue generates a suitable pinna structure for autologous ear reconstruction.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"571-582"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CTG18.1 expansion in transcription factor 4 (TCF4) in corneal graft failure: preliminary study. 角膜移植失败中转录因子 4 (TCF4) 的 CTG18.1 扩增：初步研究。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2024-01-11 DOI: 10.1007/s10561-023-10123-y

Ida Maria Westin, Andreas Viberg, Irina Golovleva, Berit Byström

{"title":"CTG18.1 expansion in transcription factor 4 (TCF4) in corneal graft failure: preliminary study.","authors":"Ida Maria Westin, Andreas Viberg, Irina Golovleva, Berit Byström","doi":"10.1007/s10561-023-10123-y","DOIUrl":"10.1007/s10561-023-10123-y","url":null,"abstract":"Fuchs endothelial corneal dystrophy (FECD) is caused by a corneal endothelial cell loss, leading to corneal edema and visual impairment. The most significant genetic risk factor for FECD is an expansion of the CTG18.1 locus in transcription factor 4 (TCF4). The current treatment for severe FECD is corneal transplantation, with Descemet stripping automated keratoplasty (DSAEK) as a common surgical method. Although successful in most cases, the risk for transplant failure due to diverse causes must be considered. In this study, we investigated if presence of TCF4 CTG18.1 expansion with more than 31 (n ≥ 31) repeats in donated corneal grafts could be a reason for corneal transplant failure after DSAEK. For this, nine consecutively failed DSAEK corneal grafts were genotyped for CTG18.1 repeat length. One-sided Mann-Whitney U test was performed to evaluate if failed DSAEK corneal grafts had longer CTG18.1 repeats than healthy controls from the same population. All failed corneal grafts had CTG18.1 n ≤ 27 with a median of 18 (IQR 8.0) repeats for the longest allele. There was no statistical difference in CTG18.1 repeat lengths between failed corneal grafts and the geographically matched healthy control group. In conclusion, none of the nine failed corneal grafts in our material had CTG18.1 repeat lengths ≥ 31, a cut-off known to have a biological relevance in FECD. Thus, our results suggest that the assessment of donors and inspection of the corneal tissue before the decision for procurement is sufficient, in terms of recognizing FECD in the donor.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"613-618"},"PeriodicalIF":1.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11142935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139416445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of bone marrow-derived mesenchymal stem cells with eggshell membrane for full-thickness wound healing in a rabbit model. 评估骨髓间充质干细胞与蛋壳膜在兔子模型中用于全厚伤口愈合的效果。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-08-04 DOI: 10.1007/s10561-023-10105-0

S Amitha Banu, Abhijit M Pawde, Khan Sharun, E Kalaiselvan, Shivaraju Shivaramu, Karikalan Mathesh, Vikas Chandra, Rohit Kumar, Swapan Kumar Maiti, Med Ram Verma, Karam Pal Singh, Amarpal

{"title":"Evaluation of bone marrow-derived mesenchymal stem cells with eggshell membrane for full-thickness wound healing in a rabbit model.","authors":"S Amitha Banu, Abhijit M Pawde, Khan Sharun, E Kalaiselvan, Shivaraju Shivaramu, Karikalan Mathesh, Vikas Chandra, Rohit Kumar, Swapan Kumar Maiti, Med Ram Verma, Karam Pal Singh, Amarpal","doi":"10.1007/s10561-023-10105-0","DOIUrl":"10.1007/s10561-023-10105-0","url":null,"abstract":"Biomaterials capable of managing wounds should have essential features like providing a natural microenvironment for wound healing and as support material for stimulating tissue growth. Eggshell membrane (ESM) is a highly produced global waste due to increased egg consumption. The unique and fascinating properties of ESM allow their potential application in tissue regeneration. The wound healing capacity of bone marrow-derived mesenchymal stem cells (BM-MSCs), ESM, and their combination in rabbits with full-thickness skin defect (2 × 2 cm2) was evaluated. Twenty-five clinically healthy New Zealand White rabbits were divided into five groups of five animals each, with group A receiving no treatment (control group), group B receiving only fibrin glue (FG), group C receiving FG and ESM as a dressing, group D receiving FG and BM-MSCs, and group E receiving a combination of FG, ESM, and BM-MSCs. Wound healing was assessed using clinical, macroscopical, photographic, histological, histochemical, hematological, and biochemical analysis. Macroscopic examination of wounds revealed that healing was exceptional in group E, followed by groups D and C, compared to the control group. Histopathological findings revealed improved quality and a faster rate of healing in group E compared to groups A and B. In addition, healing in group B treated with topical FG alone was nearly identical to that in control group A. However, groups C and D showed improved and faster recovery than control groups A and B. The macroscopic, photographic, histological, and histochemical evaluations revealed that the combined use of BM-MSCs, ESM, and FG had superior and faster healing than the other groups.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"493-508"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9942954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of secretomes in cell-free therapeutic strategies in regenerative medicine. 分泌物在再生医学无细胞治疗策略中的作用。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-02-01 DOI: 10.1007/s10561-023-10073-5

Aida Karimian, Seyedeh Mahdieh Khoshnazar, Tahmineh Kazemi, Asadollah Asadi, Arash Abdolmaleki

{"title":"Role of secretomes in cell-free therapeutic strategies in regenerative medicine.","authors":"Aida Karimian, Seyedeh Mahdieh Khoshnazar, Tahmineh Kazemi, Asadollah Asadi, Arash Abdolmaleki","doi":"10.1007/s10561-023-10073-5","DOIUrl":"10.1007/s10561-023-10073-5","url":null,"abstract":"After an injury, peripheral nervous system neurons have the potential to rebuild their axons by generating a complicated activation response. Signals from the damaged axon are required for this genetic transition to occur. Schwann cells (SCs) near a damaged nerve's distal stump also play a role in the local modulation of axonal programs, not only via cell-to-cell contacts but also through secreted signals (the secretome). The secretome is made up of all the proteins that the cell produces, such as cytokines, growth factors, and extracellular vesicles. The released vesicles may carry signaling proteins as well as coding and regulatory RNAs, allowing for multilayer communication. The secretome of SCs is now well understood as being critical for both orchestrating Wallerian degeneration and maintaining axonal regeneration. As a consequence, secretome has emerged as a feasible tissue regeneration alternative to cell therapy. Separate SC secretome components have been used extensively in the lab to promote peripheral nerve regeneration after injury. However, in neurological therapies, the secretome generated by mesenchymal (MSC) or other derived stem cells has been the most often used. In fact, the advantages of cell treatment have been connected to the release of bioactive chemicals and extracellular vesicles, which make up MSCs' secretome.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"411-426"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9153692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decellularized cryopreserved human pericardium: a validation study towards tissue bank practice. 脱细胞低温保存人心包：面向组织库实践的验证研究。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-01-25 DOI: 10.1007/s10561-023-10072-6

Giulia Montagner, Antonia Barbazza, Andrea Tancredi Lugas, Mara Terzini, Gianpaolo Serino, Cristina Bignardi, Matilde Cacciatore, Vladimiro L Vida, Massimo A Padalino, Diletta Trojan

{"title":"Decellularized cryopreserved human pericardium: a validation study towards tissue bank practice.","authors":"Giulia Montagner, Antonia Barbazza, Andrea Tancredi Lugas, Mara Terzini, Gianpaolo Serino, Cristina Bignardi, Matilde Cacciatore, Vladimiro L Vida, Massimo A Padalino, Diletta Trojan","doi":"10.1007/s10561-023-10072-6","DOIUrl":"10.1007/s10561-023-10072-6","url":null,"abstract":"Pericardial patches are currently used as reconstructive material in cardiac surgery for surgical treatment of cardiac septal defects. Autologous pericardial patches, either treated with glutaraldehyde or not, can be used as an alternative to synthetic materials or xenograft in congenital septal defects repair. The availability of an allogenic decellularized pericardium could reduce complication during and after surgery and could be a valid alternative. Decellularization of allogenic tissues aims at reducing the immunogenic reaction that might trigger inflammation and tissue calcification over time. The ideal graft for congenital heart disease repair should be biocompatible, mechanically resistant, non-immunogenic, and should have the ability to growth with the patients. The aim of the present study is the evaluation of the efficacy of a new decellularization protocol of homologous pericardium, even after cryopreservation. The technique has proven to be suitable as a tissue bank procedure and highly successful in the removal of cells and nucleic acids content, but also in the preservation of collagen and biomechanical properties of the human pericardium.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"401-410"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11142958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10619623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of caffeine intake and passive smoking on umbilical cord blood unit's quality parameters. 咖啡因摄入和被动吸烟对脐血单位质量参数的影响。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-10-15 DOI: 10.1007/s10561-023-10111-2

Walid Almashaqbeh, Husam Ardah, Amal Alasmari, Anadel Hakeem, Shahad Alsaigh, Amairah Aloushan, Dunia Jawdat

{"title":"The effect of caffeine intake and passive smoking on umbilical cord blood unit's quality parameters.","authors":"Walid Almashaqbeh, Husam Ardah, Amal Alasmari, Anadel Hakeem, Shahad Alsaigh, Amairah Aloushan, Dunia Jawdat","doi":"10.1007/s10561-023-10111-2","DOIUrl":"10.1007/s10561-023-10111-2","url":null,"abstract":"Today cord blood (CB) is a valuable source of hematopoietic stem cells to treat many hematological disorders. One of the limitations of CB utilization is the reduced number of nucleated cells including stem cells. Therefore, CB banks around the world have developed strategies in an attempt to improve donor selection and the quality of the CB inventory. This study aimed to determine the impact of passive smoking and caffeine consumption on CB quality. CBs were obtained from mothers who gave birth at King Abdulaziz Medical City. All mothers gave their informed consent. Personal interviews about the mother's demographics, smoking status and exposure, and caffeine consumption executed, followed by a chart review to analyze maternal and neonatal factors. Laboratory testing was performed on all collected CB units. Using descriptive statistics, maternal and newborn factors were analyzed. T-test or Mann-Whitney U Test, as appropriate, for continuous variables analysis to study the effect of second hand smoking and coffee consumption for the primary outcome. Our study demonstrated a reduction in CB MNC, including lymphocytes, in caffeine consumers among pregnant donors, as well as a reduction in cell potency activities, including total CFU and BFU-E. The effect of passive cigarette smoking on the same cohort was insignificant. Outcome of this study will help in optimizing the quality and quantity of stem cell harvesting from CB to get the maximum benefit and such knowledge will raise the awareness among pregnant women.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"541-547"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomechanical properties of articular cartilage in different regions and sites of the knee joint: acquisition of osteochondral allografts. 膝关节不同区域和部位关节软骨的生物力学特性：骨软骨异体移植的获得。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2024-02-06 DOI: 10.1007/s10561-024-10126-3

Yongsheng Ma, Qitai Lin, Xueding Wang, Yang Liu, Xiangyang Yu, Zhiyuan Ren, Yuanyu Zhang, Li Guo, Xiaogang Wu, Xiangyu Zhang, Pengcui Li, Wangping Duan, Xiaochun Wei

{"title":"Biomechanical properties of articular cartilage in different regions and sites of the knee joint: acquisition of osteochondral allografts.","authors":"Yongsheng Ma, Qitai Lin, Xueding Wang, Yang Liu, Xiangyang Yu, Zhiyuan Ren, Yuanyu Zhang, Li Guo, Xiaogang Wu, Xiangyu Zhang, Pengcui Li, Wangping Duan, Xiaochun Wei","doi":"10.1007/s10561-024-10126-3","DOIUrl":"10.1007/s10561-024-10126-3","url":null,"abstract":"Osteochondral allograft (OCA) transplantation involves grafting of natural hyaline cartilage and supporting subchondral bone into the cartilage defect area to restore its biomechanical and tissue structure. However, differences in biomechanical properties and donor-host matching may impair the integration of articular cartilage (AC). This study analyzed the biomechanical properties of the AC in different regions of different sites of the knee joint and provided a novel approach to OCA transplantation. Intact stifle joints from skeletally mature pigs were collected from a local abattoir less than 8 h after slaughter. OCAs were collected from different regions of the joints. The patella and the tibial plateau were divided into medial and lateral regions, while the trochlea and femoral condyle were divided into six regions. The OCAs were analyzed and compared for Young's modulus, the compressive modulus, and cartilage thickness. Young's modulus, cartilage thickness, and compressive modulus of OCA were significantly different in different regions of the joints. A negative correlation was observed between Young's modulus and the proportion of the subchondral bone (r = - 0.4241, P < 0.0001). Cartilage thickness was positively correlated with Young's modulus (r = 0.4473, P < 0.0001) and the compressive modulus (r = 0.3678, P < 0.0001). During OCA transplantation, OCAs should be transplanted in the same regions, or at the closest possible regions to maintain consistency of the biomechanical properties and cartilage thickness of the donor and recipient, to ensure smooth integration with the surrounding tissue. A 7 mm depth achieved a higher Young's modulus, and may represent the ideal length.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"633-648"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11143059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139691322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preanalytical variables influencing the interpretation and reporting of biological tests on blood samples of living and deceased donors for human body materials. 影响活体和已故人体材料捐献者血液样本生物检测的解释和报告的分析前变量。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-08-25 DOI: 10.1007/s10561-023-10106-z

Elizaveta Padalko, Luc Colenbie, Alain Delforge, Nadine Ectors, Johan Guns, Romain Imbert, Hilde Jansens, Jean-Paul Pirnay, Marie-Pierre Rodenbach, Ivan Van Riet, Anne Vansteenbrugge, Gilbert Verbeken, Muriel Baltes, Hilde Beele

{"title":"Preanalytical variables influencing the interpretation and reporting of biological tests on blood samples of living and deceased donors for human body materials.","authors":"Elizaveta Padalko, Luc Colenbie, Alain Delforge, Nadine Ectors, Johan Guns, Romain Imbert, Hilde Jansens, Jean-Paul Pirnay, Marie-Pierre Rodenbach, Ivan Van Riet, Anne Vansteenbrugge, Gilbert Verbeken, Muriel Baltes, Hilde Beele","doi":"10.1007/s10561-023-10106-z","DOIUrl":"10.1007/s10561-023-10106-z","url":null,"abstract":"With the present paper, the Working Group on Cells, Tissues and Organs and other experts of the Superior Health Council of Belgium aimed to provide stakeholders in material of human origin with advice on critical aspects of serological and nucleic acid test (NAT) testing, to improve virological safety of cell- and tissue and organ donation. The current paper focusses on a number of preanalytical variables which can be critical for any medical biology examination: (1) sampling related variables (type of samples, collection of the samples, volume of the sample, choice of specific tubes, identification of tubes), (2) variables related to transport, storage and processing of blood samples (transport, centrifugation and haemolysis, storage before and after centrifugation, use of serum versus plasma), (3) variables related to dilution (haemodilution, pooling of samples), and (4) test dependent variables (available tests and validation). Depending on the type of donor (deceased donor (heart-beating or non-heart beating) versus living donor (allogeneic, related, autologous), and the type of donated human material (cells, tissue or organs) additional factors can play a role: pre- and post-mortem sampling, conditions of sampling (e.g. morgue), haemodilution, possibility of retesting.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"509-520"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11143040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10065489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activated platelet-rich plasma accelerate endometrial regeneration and improve pregnancy outcomes in murine model of disturbed endometrium. 激活的富血小板血浆可加速子宫内膜再生，改善子宫内膜紊乱小鼠模型的妊娠结局。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-07-19 DOI: 10.1007/s10561-023-10101-4

Jeevitaa Kshersagar, Akshay A Kawale, Kishor Tardalkar, Mrunal N Damle, Leena R Chaudhari, Rakesh Sharma, Meghnad G Joshi

{"title":"Activated platelet-rich plasma accelerate endometrial regeneration and improve pregnancy outcomes in murine model of disturbed endometrium.","authors":"Jeevitaa Kshersagar, Akshay A Kawale, Kishor Tardalkar, Mrunal N Damle, Leena R Chaudhari, Rakesh Sharma, Meghnad G Joshi","doi":"10.1007/s10561-023-10101-4","DOIUrl":"10.1007/s10561-023-10101-4","url":null,"abstract":"Platelet Rich Plasma (PRP) contains high concentrations of growth factors, therefore, PRP activation results in their release, stimulating the process of healing and regeneration. The study was conducted to check whether activated platelet-rich plasma (aPRP) treatment can improve regeneration of the endometrium in an experimental model of ethanol-induced disturbed endometrium. Seventy-two female Wistar rats were randomly assigned into the control group, disturbed endometrium (DE) group and aPRP treated group. Activation of PRP was performed by adding thrombin. All the animals were sacrificed on day 1, day 3, day 6 and day 9 and samples were taken from the miduterine horn. Quantification of Cytokine and chemokine profiles of activated and non-activated PRP for CCL2, TNF- α, IL-1β, CXCL8, CXCL10, IL2, IL4, IL-6 IL-10, IL-12, IL-17A, TGF- β, IFN-γ was carried out. Functional and structural recovery of the endometrium was analyzed by hematoxylin-eosin (HE) and immunohistochemical (IHC) analyses. HE confirmed proliferated epithelial lining and stromal reconstruction with decreased fibrosis in PRP treated group compared to the DE group. Epithelial thickness in aPRP treated on day 1, day 3, day 6 and day 9 revealed an significant increase (p ≤ 0.05). Significantly stronger IHC expression of alpha smooth muscle actin, Cytokeratin 18, Cytokeratin 19, Connexin-40, E-Cadherin, Claudin-1, Zona Occludin-1was found in the aPRP treated group compared to the DE group. Furthermore, aPRP treatment was associated with birth of live pups. Our results suggest that intrauterine administration of aPRP stimulated and accelerated the regeneration of endometrium in the murine model of disturbed endometrium.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"453-461"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9838199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0