The effect of caffeine intake and passive smoking on umbilical cord blood unit's quality parameters.

IF 1.4 4区 医学 Q4 CELL BIOLOGY
Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-10-15 DOI:10.1007/s10561-023-10111-2
Walid Almashaqbeh, Husam Ardah, Amal Alasmari, Anadel Hakeem, Shahad Alsaigh, Amairah Aloushan, Dunia Jawdat
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引用次数: 0

Abstract

Today cord blood (CB) is a valuable source of hematopoietic stem cells to treat many hematological disorders. One of the limitations of CB utilization is the reduced number of nucleated cells including stem cells. Therefore, CB banks around the world have developed strategies in an attempt to improve donor selection and the quality of the CB inventory. This study aimed to determine the impact of passive smoking and caffeine consumption on CB quality. CBs were obtained from mothers who gave birth at King Abdulaziz Medical City. All mothers gave their informed consent. Personal interviews about the mother's demographics, smoking status and exposure, and caffeine consumption executed, followed by a chart review to analyze maternal and neonatal factors. Laboratory testing was performed on all collected CB units. Using descriptive statistics, maternal and newborn factors were analyzed. T-test or Mann-Whitney U Test, as appropriate, for continuous variables analysis to study the effect of second hand smoking and coffee consumption for the primary outcome. Our study demonstrated a reduction in CB MNC, including lymphocytes, in caffeine consumers among pregnant donors, as well as a reduction in cell potency activities, including total CFU and BFU-E. The effect of passive cigarette smoking on the same cohort was insignificant. Outcome of this study will help in optimizing the quality and quantity of stem cell harvesting from CB to get the maximum benefit and such knowledge will raise the awareness among pregnant women.

咖啡因摄入和被动吸烟对脐血单位质量参数的影响。
今天,脐血(CB)是治疗许多血液系统疾病的有价值的造血干细胞来源。CB利用的限制之一是包括干细胞在内的有核细胞的数量减少。因此,世界各地的CB银行都制定了战略,试图改善捐助者的选择和CB库存的质量。本研究旨在确定被动吸烟和咖啡因摄入对CB质量的影响。CBs是从在阿卜杜勒阿齐兹国王医疗城分娩的母亲那里获得的。所有母亲都表示知情同意。对母亲的人口统计、吸烟状况和接触情况以及咖啡因消费情况进行了个人访谈,然后进行了图表审查,以分析母亲和新生儿的因素。对所有收集的CB单元进行了实验室测试。采用描述性统计方法,对孕产妇和新生儿因素进行分析。T检验或Mann-Whitney U检验,视情况而定,用于连续变量分析,以研究二手烟和咖啡消费对主要结果的影响。我们的研究表明,在妊娠供体中,咖啡因消费者的CB MNC(包括淋巴细胞)减少,细胞效力活性(包括总CFU和BFU-E)减少。被动吸烟对同一人群的影响并不显著。这项研究的结果将有助于优化CB干细胞采集的质量和数量,以获得最大效益,这些知识将提高孕妇的意识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell and Tissue Banking
Cell and Tissue Banking CELL BIOLOGY-ENGINEERING, BIOMEDICAL
CiteScore
3.10
自引率
13.30%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Cell and Tissue Banking provides a forum for disseminating information to scientists and clinicians involved in the banking and transplantation of cells and tissues. Cell and Tissue Banking is an international, peer-reviewed journal that publishes original papers in the following areas: basic research concerning general aspects of tissue banking such as quality assurance and control of banked cells/tissues, effects of preservation and sterilisation methods on cells/tissues, biotechnology, etc.; clinical applications of banked cells/tissues; standards of practice in procurement, processing, storage and distribution of cells/tissues; ethical issues; medico-legal issues.
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