Central European Journal of Immunology最新文献_第2页

LncRNA HCP5 acts as a potential diagnostic biomarker and attenuates the inflammatory response in neonatal sepsis by targeting miR-138-5p/SIRT1.

IF 1.5 4区医学

Central European Journal of Immunology Pub Date : 2024-01-01 Epub Date: 2024-09-27 DOI: 10.5114/ceji.2024.143462

Xiaohua Hu, Anhui Hu, Yong Luo, Shuisheng Yuan, Lei Yang

{"title":"LncRNA HCP5 acts as a potential diagnostic biomarker and attenuates the inflammatory response in neonatal sepsis by targeting miR-138-5p/SIRT1.","authors":"Xiaohua Hu, Anhui Hu, Yong Luo, Shuisheng Yuan, Lei Yang","doi":"10.5114/ceji.2024.143462","DOIUrl":"10.5114/ceji.2024.143462","url":null,"abstract":"Introduction: This study aimed to investigate the clinical significance and potential mechanism of long non-coding RNA human histocompatibility leukocyte antigen complex P5 (HCP5) in neonatal sepsis (NS).Material and methods: The study enrolled 86 patients with NS and 80 neonates with respiratory tract infection or pneumonia. The Pearson correlation coefficient was used to evaluate the association of procalcitonin (PCT), C-reactive protein (CRP), and inflammatory factors with HCP5. Serum levels of HCP5 were measured using RT-qPCR. The diagnostic potential of HCP5 was assessed via a receiver operating characteristic (ROC) curve. An in vitro model was established using lipopolysaccharide (LPS)-induced RAW264.7 macrophages. ELISA was conducted to measure the levels of inflammatory factors. Finally, the target relationship was validated using a dual-luciferase reporter assay.Results: HCP5 was significantly lower in patients with NS and it negatively correlated with PCT, CRP, interleukin (IL)-8, and tumor necrosis factor α (TNF-α). The area under the ROC curve was 0.902, and the sensitivity and specificity for identifying NS from controls were 86.30% and 83.72%, respectively. In LPS-induced RAW264.7, the levels of HCP5 decreased in a time- and dose-dependent manner. miR-138-5p, a target miRNA for HCP5, was found to be elevated in NS patients. Furthermore, HCP5 significantly reduced LPS-induced overproduction of inflammatory factors, but miR-138-5p reversed this reduction. Furthermore, sirtuin 1 (SIRT1) is a downstream target of miR-138-5p.Conclusions: HCP5 could potentially serve as a diagnostic biomarker for NS and it may inhibit inflammation in NS by targeting miR-138-5p/SIRT1 axis. These findings highlight the potential role of HCP5 in the diagnosis and treatment of NS.","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"49 3","pages":"216-226"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term efficacy of mesenchymal stem cell treatment for complex perianal fistulas: A systematic review and meta-analysis.

IF 1.5 4区医学

Central European Journal of Immunology Pub Date : 2024-01-01 Epub Date: 2024-11-12 DOI: 10.5114/ceji.2024.144866

Tao Wang, Min Li, Hua Shang, Lei Zou, Feng Shang

{"title":"Long-term efficacy of mesenchymal stem cell treatment for complex perianal fistulas: A systematic review and meta-analysis.","authors":"Tao Wang, Min Li, Hua Shang, Lei Zou, Feng Shang","doi":"10.5114/ceji.2024.144866","DOIUrl":"10.5114/ceji.2024.144866","url":null,"abstract":"Introduction: Complex perianal fistula, which is characterized by high occurrence and is difficult to treat with current surgical techniques, negatively affects the life quality of patients. Mesenchymal stem cells (MSCs) have emerged as a new innovative therapy in recent years due to their potent anti-inflammatory and immunomodulatory properties. Considering the high recurrence rate of complex perianal fistula, we performed this systematic review and meta-analysis to assess the long-term effects of MSCs on complex perianal fistula.Material and methods: Trials with MSC treatment for complex perianal fistula were included. Analyses were conducted using Stata software. The Egger test for linear regression and Begg's funnel plot were used.Results: MSC treatment considerably improved the clinical response of complex perianal fistula at a follow-up of 24 weeks (OR = 1.86, 95% CI: 1.39-2.50), 48 weeks (OR = 2.23, 95% CI: 1.52-3.26), and 96 weeks (OR = 2.08, 95% CI: 1.17-3.68).Conclusions: MSC therapy has a long-term effect on the clinical response of complex perianal fistula and should be widely promoted not only in adults but also in infants and adolescents; however, more research on this topic is needed.","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"49 3","pages":"273-281"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel IL2RG gene mutation causing primary combined immunodeficiency disease: A case report and literature review.

IF 1.5 4区医学

Central European Journal of Immunology Pub Date : 2024-01-01 Epub Date: 2024-09-20 DOI: 10.5114/ceji.2024.142340

Fang Cao, Yingyu Shi, Fang Deng, Yu Yan

{"title":"Novel IL2RG gene mutation causing primary combined immunodeficiency disease: A case report and literature review.","authors":"Fang Cao, Yingyu Shi, Fang Deng, Yu Yan","doi":"10.5114/ceji.2024.142340","DOIUrl":"10.5114/ceji.2024.142340","url":null,"abstract":"This study presents a detailed clinical case of a 10-year-old boy with a history of prolonged cough, fever, and delayed diagnosis of bronchiectasis. Review of the case revealed that the child has had recurrent bronchitis, otitis media, skin allergies, and viral warts since early childhood, indicating persistent immune system abnormalities. Imaging studies, including pulmonary and sinus CT scans, show significant bronchiectasis accompanied by infections and sinusitis. Immunological assessment revealed abnormalities in immunoglobulin levels and T-cell distribution, suggesting a potential immune deficiency. Whole exome sequencing did not identify any genetic variants highly associated with and definitively pathogenic for bronchiectasis but detected a compound heterozygous missense mutation c.420A>T (p.R140S) in the IL2RG gene, linked to primary combined immunodeficiency (CID), a clinical phenotype rarely reported in China due to this gene mutation. This case report not only enhances our understanding of CID but also provides a new addition to the genetic landscape of CID both domestically and internationally, aiding in earlier diagnosis and treatment of such diseases in clinical practice. During the 18-month follow-up period, the child was unable to participate in physical activities, and experienced recurrent rhinitis, sinusitis, and warts. The child's current weight and height are 30 kg and 140 cm, respectively.","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"49 3","pages":"300-307"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apurinic/apyrimidinic endonuclease 1 alleviates inflammation in fibroblast-like synoviocytes from patients with rheumatoid arthritis. Apurinic/apyrimidinic endonuclease 1 可减轻类风湿性关节炎患者成纤维细胞样滑膜细胞的炎症反应。

IF 1.5 4区医学

Central European Journal of Immunology Pub Date : 2024-01-01 Epub Date: 2024-08-26 DOI: 10.5114/ceji.2024.141946

Ha-Reum Lee, Su-Jin Yoo, Jinhyun Kim, Yu Ran Lee, Hee Kyoung Joo, Byeong Hwa Jeon, Seong Wook Kang

{"title":"Apurinic/apyrimidinic endonuclease 1 alleviates inflammation in fibroblast-like synoviocytes from patients with rheumatoid arthritis.","authors":"Ha-Reum Lee, Su-Jin Yoo, Jinhyun Kim, Yu Ran Lee, Hee Kyoung Joo, Byeong Hwa Jeon, Seong Wook Kang","doi":"10.5114/ceji.2024.141946","DOIUrl":"https://doi.org/10.5114/ceji.2024.141946","url":null,"abstract":"Introduction: Apurinic/apyrimidinic endonuclease 1 (APEX1) is a protein with elevated expression in synovial fluids from rheumatoid arthritis (RA) patients. However, its role in RA pathogenesis remains unexplored. This study investigated the influence of APEX1 on inflammatory pathways in fibroblast-like synoviocytes (FLS) isolated from RA patients.Material and methods: FLS from RA patients (n = 5) were stimulated with recombinant tumor necrosis factor α (TNF-α) and interleukin (IL)-17. Subsequently, cells were treated with recombinant APEX1, and assessments were made on reactive oxygen species (ROS) production and mitochondrial membrane potential. Additionally, mRNA levels of IL-1 family members were quantified. Cell migration was evaluated through Transwell chamber assays, and levels of key secreted inflammatory cytokines were measured via enzyme-linked immunosorbent assay (ELISA).Results: The results demonstrated that APEX1 significantly reduced mitochondrial-specific ROS expression and restored mitochondrial membrane potential in TNF-α/IL-17-stimulated RA FLS. Furthermore, APEX1 treatments attenuated TNF-α/IL-17-induced activation of p38 MAPK, NF-κB, and PI3K 110 δ signaling pathways. Similarly, APEX1 significantly diminished TNF-α/IL-17-induced expression of inflammatory cytokines, including IL-1 family members, IL-6, IL-8, and vascular endothelial growth factor (VEGF). Notably, APEX1 downregulated cell migration of TNF-α/IL-17-treated RA FLS via inhibition of matrix metalloproteinase 3 (MMP3).Conclusions: These findings collectively underscore the role of APEX1 as a key mediator of cytokine-amplified migration, modulating ROS and MMP3 in RA FLS, thus supporting its potential as a therapeutic target in RA treatment.","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"49 2","pages":"113-125"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142388338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in immune status of circulating NK cells in patients with latent tuberculosis infection. 潜伏肺结核感染患者循环 NK 细胞免疫状态的变化。

IF 1.5 4区医学

Central European Journal of Immunology Pub Date : 2024-01-01 Epub Date: 2024-08-12 DOI: 10.5114/ceji.2024.142104

Shuang Qin, Ruiqi Chen, Meihui Li, Jiangfeng Lv, Fengmei Zhang, Yuguo Ren, Xiangyang Lin

{"title":"Changes in immune status of circulating NK cells in patients with latent tuberculosis infection.","authors":"Shuang Qin, Ruiqi Chen, Meihui Li, Jiangfeng Lv, Fengmei Zhang, Yuguo Ren, Xiangyang Lin","doi":"10.5114/ceji.2024.142104","DOIUrl":"https://doi.org/10.5114/ceji.2024.142104","url":null,"abstract":"Introduction: The presence of patients with latent tuberculosis infection (LTBI) has fueled the tuberculosis pandemic. We aimed to investigate the immune status of NK cells in LTBI patients.Material and methods: Twenty-one LTBI patients, 25 active pulmonary tuberculosis (APTB) patients and 25 healthy controls (HCs) participated in our research. The markers of NK cells were detected by flow cytometry.Results: The absolute number of circulating CD56bright and CD56dim NK cells in LTBI patients was higher than that of APTB patients, but the frequency of HLA-DR+ CD56bright NK cells was significantly lower than that of HCs and APTB patients. Also, LTBI patients with CD56bright NK cells had intracellular levels of granzyme B that were as significantly elevated as those with APTB patients, but the levels of granzyme A and perforin were reduced. Meanwhile, the frequencies of CXCR3+ NK cells, CXCR3+ CD56bright and CXCR3+ CD56dim NK cells were significantly lower in LTBI patients.Conclusions: Circulating CD56bright NK cells exerted a significant role in maintaining immune balance in LTBI patients. An elevated frequency of granzyme B+ CD56bright NK cells and a reduced frequency of perforin+ CD56bright NK cells were effective in differentiating LTBI patients from HCs.","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"49 2","pages":"105-112"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142388340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting the serum marker interleukin 9 improves the underlying characterization and immune homeostasis in rheumatoid arthritis. 以血清标志物白细胞介素 9 为靶标，可改善类风湿性关节炎的基本特征和免疫稳态。

IF 1.5 4区医学

Central European Journal of Immunology Pub Date : 2024-01-01 Epub Date: 2024-08-26 DOI: 10.5114/ceji.2024.141695

Ying Xiong, Wang Xiang, Wei Xiao

{"title":"Targeting the serum marker interleukin 9 improves the underlying characterization and immune homeostasis in rheumatoid arthritis.","authors":"Ying Xiong, Wang Xiang, Wei Xiao","doi":"10.5114/ceji.2024.141695","DOIUrl":"https://doi.org/10.5114/ceji.2024.141695","url":null,"abstract":"Introduction: Rheumatoid factor (RF) and anti-citrullinated peptide antibodies (ACPA) are serological markers used for diagnosing rheumatoid arthritis (RA), an autoimmune disease characterized by inflammatory joint damage. However, there is a subset of RA patients who test negative for both RF and ACPA, known as seronegative rheumatoid arthritis (SNRA).Material and methods: The levels of serum markers were examined in both clinical samples and a rat model of type II collagen-induced RA (CIA). The effect of interleukin 9 (IL-9) on RA was investigated using recombinant rat IL-9 (rrIL-9), anti-rat IL-9 neutralizing monoclonal antibody (mAb), and control IgG antibody in the CIA rat. The severity of arthritis was assessed. Treg and Th17 cells, M1 and M2 macrophages, and inflammatory cytokine levels were analyzed.Results: We observed higher levels of IL-9 in clinical samples from SNRA patients compared to the normal group. Rat models of CIA exhibit increased arthritis scores, weight loss, paw swelling, and severe joint damage. IL-9 was the most sensitive serum marker for the diagnosis of RA in serum assays of CIA rats. IL-9 increased arthritis scores and cartilage damage, whereas treatment with IL-9 inhibitors produced the opposite effect. IL-9 inhibitors promoted Treg/Th17 homeostasis, decreased M1 macrophages, increased M2 macrophages, and decreased levels of inflammatory cytokines in joint tissues.Conclusions: These results suggest that IL-9 has potential as a diagnostic marker for SNRA. Inhibition of IL-9 could reduce the severity of arthritis in CIA rats by ameliorating inflammation and modulating the Treg/Th17 immune balance, M2 and M1 macrophage activation.","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"49 2","pages":"132-146"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142388346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influenza vaccination as a prognostic factor of humoral IgA responses to SARS-CoV-2 infection. 流感疫苗接种是 SARS-CoV-2 感染体液 IgA 反应的预后因素。

IF 1.3 4区医学

Central European Journal of Immunology Pub Date : 2024-01-01 Epub Date: 2024-02-17 DOI: 10.5114/ceji.2024.135462

Barbara Poniedziałek, Dominika Sikora, Ewelina Hallmann, Lidia Brydak, Piotr Rzymski

{"title":"Influenza vaccination as a prognostic factor of humoral IgA responses to SARS-CoV-2 infection.","authors":"Barbara Poniedziałek, Dominika Sikora, Ewelina Hallmann, Lidia Brydak, Piotr Rzymski","doi":"10.5114/ceji.2024.135462","DOIUrl":"10.5114/ceji.2024.135462","url":null,"abstract":"There is evidence that influenza vaccination may provide additional benefits by inducing training of innate immunity and increasing humoral responses to heterologous challenges. Immunoglobulin A (IgA) antibodies dominate the early phase of the adaptive response to SARS-CoV-2 infection, but whether their production may be associated with previous influenza vaccination has not been a subject of any study. This study compared serum SARS-CoV-2-specific IgA responses, measured with Microblot-Array assay, in individuals who experienced COVID-19 (N = 1318) and differed in the status of the seasonal influenza vaccine, age, sex, and disease severity. Influenza-vaccinated individuals had a higher seroprevalence of IgA antibodies against nucleocapsid (anti-NP; by 10.1%), receptor-binding domain of spike protein (anti-RBD; by 11.8%) and the S2 subunit of spike protein (anti-S2; by 6.8%). Multivariate analysis, including age, sex, and COVID-19 severity, confirmed that receiving the influenza vaccine was associated with higher odds of being seropositive for anti-NP (OR, 95% CI = 1.57, 1.2-2.0), anti-RBD (OR, 95% CI = 1.6, 1.3-2.0), and anti-S2 (OR, 95% CI = 1.9, 1.4-2.7), as well as being seropositive for at least one anti-SARS-CoV-2 IgA antibody (OR, 95% CI = 1.7, 1.3-2.1) and all three of them (OR, 95% CI = 2.6, 1.7-4.0). Age ≥ 50 years was an additional factor predicting better IgA responses. However, the concentration of particular antibodies in seropositive subjects did not differ in relation to the influenza vaccination status. The study evidenced that influenza vaccination was associated with improved serum IgA levels produced in response to SARS-CoV-2 infection. Further studies are necessary to assess whether trained immunity is involved in the observed phenomenon.","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"49 1","pages":"11-18"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11130984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141173685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunological relationship between Helicobacter pylori and anti-tumor necrosis factor α agents in inflammatory bowel disease. 炎症性肠病中幽门螺杆菌与抗肿瘤坏死因子 α 药物之间的免疫学关系

IF 1.3 4区医学

Central European Journal of Immunology Pub Date : 2024-01-01 Epub Date: 2024-03-25 DOI: 10.5114/ceji.2024.136376

Han Huang, Chenxiao Gan, Yan Cai, Lingkang Wu

引用次数: 0

Systemic lupus erythematosus in a patient with 22q11.2 deletion syndrome: A case report and review of the literature.

IF 1.5 4区医学

Central European Journal of Immunology Pub Date : 2024-01-01 Epub Date: 2024-11-08 DOI: 10.5114/ceji.2024.143229

Chen Sun, Pingyang Han, Juzhen Yan

{"title":"Systemic lupus erythematosus in a patient with 22q11.2 deletion syndrome: A case report and review of the literature.","authors":"Chen Sun, Pingyang Han, Juzhen Yan","doi":"10.5114/ceji.2024.143229","DOIUrl":"10.5114/ceji.2024.143229","url":null,"abstract":"22q11.2 deletion syndrome (MIM: 192430/188400, ORPHA: 567) is the most common chromosomal microdeletion disorder, caused by a hemizygous microdeletion of 2.5 million base pairs on chromosome 22. There is a known association between 22q11.2 deletion syndrome (22q11.2DS), immunodeficiency and autoimmune diseases. However, the co-occurrence of 22q11.2DS and systemic lupus erythematosus (SLE) has been rarely reported. Here, we describe a case of a female teenager with distal type I 22q11.2DS who presented with alopecia, oral ulcers, fever and thrombocytopenia. Laboratory tests showed positive antinuclear antibodies (ANA) and double-stranded DNA (ds-DNA) antibodies, indicative of SLE. Treatment with prednisone, hydroxychloroquine and azathioprine resulted in improvement. We reviewed the literature on the immunological mechanisms involved in 22q11.2DS. Thymic dysplasia, T-cell lymphopenia, and B-cell abnormalities collectively contribute to the immunodeficiency and autoimmune manifestations observed in individuals with 22q11.2DS. Genetic factors such as 22q11.2DS should be considered in the diagnosis of childhood rheumatic diseases. Our case adds to the limited literature on this co-occurrence.","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"49 3","pages":"315-319"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peripheral blood lymphocyte immunophenotyping (TBNK) - a comparison of BD FACSCanto II and BD FACSLyric flow cytometry analysers. 外周血淋巴细胞免疫分型（TBNK）--BD FACSCanto II 和 BD FACSLyric 流式细胞仪的比较。

IF 1.3 4区医学

Central European Journal of Immunology Pub Date : 2024-01-01 Epub Date: 2024-03-05 DOI: 10.5114/ceji.2024.135939

Aleksandra Wiśniewska, Julia Stańczyk, Urszula Demkow, Anna Stelmaszczyk-Emmel

{"title":"Peripheral blood lymphocyte immunophenotyping (TBNK) - a comparison of BD FACSCanto II and BD FACSLyric flow cytometry analysers.","authors":"Aleksandra Wiśniewska, Julia Stańczyk, Urszula Demkow, Anna Stelmaszczyk-Emmel","doi":"10.5114/ceji.2024.135939","DOIUrl":"10.5114/ceji.2024.135939","url":null,"abstract":"Introduction: Flow cytometry immunophenotyping is a common laboratory technique for evaluating lymphocyte subpopulations. Its result remains an important diagnostic tool in various medical fields. Cytometric tests are performed in many laboratories, making the comparability between different devices using the same method an important aspect. We aimed to compare the results of lymphocyte immunophenotyping (lymphocytes B, T, Th and Tc, NK cells) between two different flow cytometers.Material and methods: The study included 93 patients of the Children's Teaching Hospital of the Medical University of Warsaw and 9 Multi-Check control results. The method of lymphocyte subpopulation assessment was based on fluorescent flow cytometry immunophenotyping, using a BD Multitest 6-color TBNK kit (Becton Dickinson). We compared BD FACSCanto II and BD FACSLyric analysers (Becton Dickinson). For data analysis, we used Spearman's rank correlation, Bland-Altman plot and Passing-Bablok regression.Results: Spearman's rank correlation showed a strong interrelation for all analysed parameters (0.808-0.985). In the Passing-Bablok regression analysis, all examined parameters showed linear dependence with the slope values close to 1 (0.940-1.134). Bland-Altman coefficient values were within the range of 2.94-8.62% with half of them being above 5% (T, Tc, Th, B, NKT absolute values and B percentage values).Conclusions: The results from both cytometers can be considered equivalent, but it should be noted that one of the statistical methods showed some deviations, presumably primarily due to the evaluators' different gating techniques. The training of specialists performing these tests requires more attention.","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"49 1","pages":"45-51"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11130988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141173688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0