Central European Journal of Immunology最新文献

{"title":"Predictive value of long non-coding RNA DDX11-AS1 in inflammatory bowel disease and its effect on intestinal mucosal cell function.","authors":"Tanwei Xiong, Xiuli Wang, Jia Li, Fangfang Li","doi":"10.5114/ceji.2025.149579","DOIUrl":"10.5114/ceji.2025.149579","url":null,"abstract":"<p><strong>Introduction: </strong>Inflammatory bowel disease (IBD) is a chronic and recurrent autoimmune condition. Numerous studies have reported that non-coding RNA, especially long non-coding RNA (lncRNA), plays a significant role in the regulation of IBD. This study sought to investigate the expression of lncRNA DDX11-antisense RNA 1 (DDX11-AS1) in IBD and its potential diagnostic value, while also evaluating the influence of DDX11-AS1 on the functionality of colorectal mucosal cells.</p><p><strong>Material and methods: </strong>The expression trend of DDX11-AS1 was determined through PCR analysis, with its clinical diagnostic value assessed via ROC curve analysis. To construct an in vitro inflammation cell model, a commercially available human normal colon epithelial cell line (FHC) was selected and induced with lipopolysaccharide (LPS). Subsequently, the CCK-8 kit, flow cytometry, and ELISA were employed to assess cell viability, apoptosis, and inflammatory responses. The target gene miR-2355-5p of DDX11-AS1 was predicted using the Encyclopedia of RNA Interactomes (ENCORI), and the interaction relationship was validated by luciferase reporting assays.</p><p><strong>Results: </strong>The study found that DDX11-AS1 expression is elevated, while miR-2355-5p expression is decreased, in patients with IBD. DDX11-AS1 demonstrated high diagnostic accuracy for IBD. In vitro, LPS exposure stimulated inflammation and apoptosis, and reduced cell viability in FHC cells. Downregulating DDX11-AS1 mitigated LPS-induced damage in these cells. Mechanistically, DDX11-AS1 was shown to directly target miR-2355-5p, exhibiting an inverse relationship.</p><p><strong>Conclusions: </strong>The study findings suggest that the upregulation of DDX11-AS1 contributes to LPS- induced apoptosis and inflammation by targeting miR-2355-5p, offering new insights into the pathogenesis of IBD.</p>","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"50 1","pages":"87-97"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of miR-192-5p as a diagnostic marker for children with severe pneumonia and respiratory failure and its predictive value for prognosis.","authors":"Jiaxi Xie, Yuting Cai, Jinkai Yang, Xiaoyan Tang, Tao Fan","doi":"10.5114/ceji.2025.149249","DOIUrl":"10.5114/ceji.2025.149249","url":null,"abstract":"<p><strong>Introduction: </strong>Severe pneumonia in children is a rapidly progressing respiratory system disease. If not promptly controlled, it can lead to respiratory failure, posing a serious threat to the child's life. This study investigated the diagnostic and prognostic potential of miR-192-5p in children with severe pneumonia and respiratory failure.</p><p><strong>Material and methods: </strong>A total of 62 children with severe pneumonia, 40 children with severe pneumonia and respiratory failure, and 62 healthy children were enrolled. The level of miR-192-5p was quantified by RT-qPCR assays. The diagnostic potential of miR-192-5p for severe pneumonia and respiratory failure was assessed through ROC curve and binary logistic analyses. The association between abnormal miR-192-5p level and prognosis of children with severe pneumonia with respiratory failure was evaluated by Kaplan-Meier and multivariate Cox analysis.</p><p><strong>Results: </strong>miR-192-5p expression was decreased in the serum of severe pneumonia and expiratory failure children. miR-192-5p has a good potential to predict the occurrence of severe pneumonia, which is a risk predictor of severe pneumonia in children with respiratory failure. In addition, downregulation of miR-192-5p was strongly associated with poor prognosis of children with severe pneumonia and respiratory failure.</p><p><strong>Conclusions: </strong>A low level of miR-192-5p has high predictive value and clinical development potential for the timely diagnosis and prediction of poor prognosis of children with severe pneumonia and respiratory failure.</p>","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"50 1","pages":"98-104"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incomplete systemic capillary leak syndrome after COVID-19 vaccination: a case-based review.","authors":"Jaroslaw Nowakowski, Brygida Marczyk, Joanna Przeniosło, Zuzanna Sawiec, Kinga Żmuda, Mariusz Korkosz","doi":"10.5114/ceji.2025.149254","DOIUrl":"10.5114/ceji.2025.149254","url":null,"abstract":"<p><p>Although generalized edema is a rare adverse event after drugs or vaccinations, here we report a case of generalized edema with systemic inflammatory reaction clinically similar to incomplete systemic capillary leak syndrome (SCLS), potentially triggered by COVID-19 immunization. The patient presented with generalized subcutaneous edema, a drop in blood pressure, elevated inflammatory biomarkers, and hypoalbuminemia after receiving the vaccine, with no other apparent cause than vaccination. Comprehensive diagnostic evaluation revealed no specific underlying cause. She was treated with glucocorticosteroids successfully. This case underscores the importance of recognizing rare but severe adverse events associated with vaccinations. Given the widespread administration of COVID-19 vaccines, it is crucial to identify and understand such reactions to ensure timely diagnosis and management. Therefore, we review the existing cases of SCLS following COVID-19 vaccination. Our review highlights the need for heightened vigilance and further research into the mechanisms underlying vaccine-induced phenomena to improve patient outcomes and vaccine safety profiles.</p>","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"50 1","pages":"119-121"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrophage M2 polarization induced by ANKRD22 in lung adenocarcinoma facilitates tumor angiogenesis.","authors":"Li Zhou, Dan Ma, Xingxing Li, Jianjiang Jin, Ting Zheng, Ningbo Zhang","doi":"10.5114/ceji.2025.149372","DOIUrl":"10.5114/ceji.2025.149372","url":null,"abstract":"<p><strong>Introduction: </strong>Lung adenocarcinoma (LUAD), the most prevalent lung cancer type, poses a great threat to public health, with its incidence and mortality rates remaining alarmingly high. While ankyrin repeat domain-containing protein 22 (ANKRD22) is linked to the development of multiple cancers, the molecular mechanisms of its impact on the malignant progression of LUAD are not yet fully understood. This study seeks to elucidate the biological role of ANKRD22 in LUAD.</p><p><strong>Material and methods: </strong>ANKRD22 expression in LUAD tissues and cells was assessed using the TCGA-LUAD database and quantitative reverse transcription polymerase chain reaction (qRT-PCR). The polarization of macrophages (derived from THP-1 cells) was examined through qRT-PCR, flow cytometry, and western blot to determine the influence of ANKRD22 on macrophage polarization. The effects of ANKRD22 knockdown on A549 cell proliferation and migration were measured using Cell Counting Kit-8 assay, colony formation, and Transwell assays. The impact of ANKRD22-induced macrophage M2 polarization on human umbilical vein endothelial cell (HUVEC) migration and angiogenesis was evaluated with Transwell and tube formation assays.</p><p><strong>Results: </strong>The expression of ANKRD22 was elevated in LUAD tissue and cellular samples, and its overexpression promoted M2 polarization in macrophages. Blocking ANKRD22-mediated M2 polarization inhibited the migration and tube formation capacity of HUVEC cells.</p><p><strong>Conclusions: </strong>Our findings showed that ANKRD22 mediates the malignant progression of LUAD by inducing M2 polarization of tumor-associated macrophages, thereby promoting angiogenesis.</p>","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"50 1","pages":"38-51"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering cardiotoxicity in PD-1/PD-L1 inhibitor treatment.","authors":"Jacek Tabarkiewicz, Eliza Głodkowska-Mrówka, Andrzej Eljaszewicz","doi":"10.5114/ceji.2025.151335","DOIUrl":"10.5114/ceji.2025.151335","url":null,"abstract":"","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"50 1","pages":"2"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innate immunosenescence and sepsis in the elderly: mechanisms and innate immune modulation strategies.","authors":"Danfeng Zhang, Jing Cheng, Donghua Cao, Kai Sheng","doi":"10.5114/ceji.2025.149291","DOIUrl":"10.5114/ceji.2025.149291","url":null,"abstract":"<p><p>This study aimed to investigate the mechanisms of innate immunosenescence in elderly patients with sepsis and to evaluate the potential application of innate immune modulation strategies in clinical management. Through a literature review, the characteristics of sepsis in the elderly, the aging mechanisms of the innate immune system, the impact of immunosenescence on susceptibility to sepsis, and clinical management strategies for sepsis in the elderly were analyzed. The incidence and mortality rates of sepsis in the elderly increase significantly with age, closely related to the severity of infection, the high prevalence of comorbidities, atypical symptoms, and a greater risk of multi-organ failure. Innate immunosenescence, including the decline in function of neutrophils, monocytes/macrophages, natural killer cells, and dendritic cells, is a key factor in the increased susceptibility to sepsis in the elderly. Immunomodulatory treatments, such as granulocyte colony-stimulating factor (G-CSF), interferon <i>γ</i> (IFN-<i>γ</i>), and granulocyte-macrophage colony-stimulating factor (GM-CSF), show potential in improving the prognosis of elderly patients with sepsis and reducing mortality rates. The management of sepsis in the elderly requires a comprehensive approach that takes into account age-related physiological and pathological changes, as well as early diagnosis and proactive intervention measures. Immunomodulatory strategies targeting the unique characteristics of immunosenescence in the elderly offer new avenues for improving survival rates and treatment outcomes in elderly patients with sepsis.</p>","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"50 1","pages":"3-10"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aberrant glucose metabolism drives dysfunction of CD4⁺ T cells in systemic lupus erythematosus and disease flares.","authors":"Lu Jin, Meng Ding, Shaoxin Cui, Lin Yang, Jinwen Zhao, Jingjing He, Xiaoping Wang, Fei Chang, Xue Liu, Qun Wang, Hongtao Jin, Jun Ma, Aijing Liu","doi":"10.5114/ceji.2025.149252","DOIUrl":"10.5114/ceji.2025.149252","url":null,"abstract":"<p><strong>Introduction: </strong>T cell immuno-metabolic regulation plays a key role in the development of systemic lupus erythematosus (SLE). This study aimed to analyze the role of CD4⁺ T cell glucose metabolism in SLE development.</p><p><strong>Material and methods: </strong>Clinical data and blood samples were collected from 20 untreated SLE patients and healthy controls (HCs) matched for age, sex, and body mass index. After being isolated by magnetic sorting and cultured with anti-CD3/CD28 for 72 h, CD4⁺ T cells were subjected to real-time metabolic analysis. CD4⁺ T cell proliferation and cytokines were measured with cell counting kit-8 and Luminex liquid chip assay, respectively.</p><p><strong>Results: </strong>Compared to HCs, SLE-CD4⁺ T cells exhibited significantly higher glycolytic capacity and mitochondrial oxidative phosphorylation (OXPHOS) (both p < 0.001). Additionally, SLE-CD4⁺ T cells demonstrated increased proliferation rates and elevated cytokine levels in both plasma and culture supernatants (both p < 0.05). OXPHOS and glycolysis of SLE-CD4⁺ T cells were positively correlated with SLE disease activity index-2000 (SLEDAI-2K) and cytokines, and negatively correlated with SLE-CD4⁺ T cell numbers (all p < 0.05).</p><p><strong>Conclusions: </strong>CD4⁺ T cells from SLE patients showed higher glucose metabolic activity than those from HCs, and the enhanced glucose metabolism of SLE-CD4⁺ T cells was strongly correlated with disease activity, suggesting that glucose metabolic reprogramming plays an essential role in the pathogenesis of SLE.</p>","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"50 1","pages":"13-23"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224248/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PD-1/PD-L1 inhibitor treatment associated with cardiotoxicity regulated by macrophage polarization and SOCS3/JAK/STAT3 signaling pathway.","authors":"Jiding Fu, Ge Wang, Lisi Zeng, Jie Lin, Yier Wei, Wei Xu, Rui Xu, Lewu Xian","doi":"10.5114/ceji.2025.149377","DOIUrl":"10.5114/ceji.2025.149377","url":null,"abstract":"<p><p>Cardiotoxicity caused by immune checkpoint inhibitors is one of the most severe and potentially fatal side effects. Hence it is crucial from a therapeutic standpoint to understand the underlying processes and devise countermeasures. This study sought to determine whether the SOCS3/JAK/STAT3 signaling pathway, which controls macrophage polarization, contributes to the cardiotoxicity caused by PD-1/PD-L1 inhibitors. The PD-1/PD-L1 inhibitor BMS-1 (10 mg/kg) was used to create a mouse model of immune checkpoint inhibitor-related cardiotoxicity, and hematoxylin and Masson's trichome tests were used to measure cardiomyocyte apoptosis and cardiotoxicity. The production of M1 factors (tumor necrosis factor <i>α</i> [TNF-<i>α</i>] and interleukin [IL]-1 <i>b</i>), as well as the blood levels of myocardial enzymes (creatine kinase, aspartate transaminase, creatine kinase-MB, and lactate dehydrogenase), were evaluated by ELISA. Echocardiography was used to assess the heart's health. The processes were investigated using flow cytometric analysis, real-time PCR, Western blot, and chromatin immunoprecipitation. We found that the PD-1/PD-L1 inhibitor BMS-1 dramatically reduced tumor weight while considerably impairing cardiac function in melanoma-induced tumor-bearing mice. At the gene and protein levels, it was found that levels of SOCS3, JAK, STAT3, and the inflammatory mediators IL-6 and TNF-<i>α</i> had all significantly decreased. Immune checkpoint inhibitor-induced cardiotoxicity may be linked to major changes in the SOCS3/JAK/STAT3 signaling pathway, as indicated by the knockdown of SOCS3, JAK, and STAT3. Finally, immune checkpoint inhibitor intervention demonstrated a large elevation of CD86+ and MHCII+ as well as a considerable increase in macrophages. These data suggest that the SOCS3/JAK/STAT3 signaling pathway, which controls macrophage polarization, may be linked to cardiotoxicity caused by PD-1/PD-L1 inhibitor therapy.</p>","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"50 1","pages":"24-37"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anifrolumab in the treatment of recurrent systemic lupus erythematosus: the first post-trial case report.","authors":"Przemysław Borowy, Alicja Kamińska, Patrycja Major, Jakub Smyk, Katarzyna Gołojuch, Bogdan Batko","doi":"10.5114/ceji.2025.148001","DOIUrl":"10.5114/ceji.2025.148001","url":null,"abstract":"<p><p>Systemic lupus erythematosus (SLE) is a chronic, multisystem autoimmune disease whose treatment is still a challenge. The latest registration of anifrolumab for the treatment of moderate-to-severe SLE raises hopes because of its novel anti-interferon mechanism of action. Anifrolumab, a human monoclonal antibody, selectively binds the interferon <i>α</i> (INF-<i>α</i>) receptor, inhibiting systemic inflammation moderated by interferon pathways. The article presents the first case of an 18-year-old man with severe, relapsing and repeatedly hospitalized SLE, successfully treated with anifrolumab. During a subsequent exacerbation with multi-organ involvement, which could not be controlled despite pulses and high doses of glucocorticoids (GCSs), treatment with anifrolumab was initiated. Clinical improvement was achieved 4 weeks after the first dose. The patient's dose of systemic GCSs was gradually reduced until complete withdrawal. No serious side effects were observed throughout the follow-up period, and the criteria for complete remission were achieved by the patient at month 3 of therapy. After 12 months, therapy was discontinued due to a payer decision. Nevertheless, the patient remains in follow-up 14 months after the completion of therapy on stable treatment with hydroxychloroquine and azathioprine. He is still not taking prednisone. This is the first case in Poland to show the fate of a \"real life patient\" after completion of anifrolumab therapy, an effective clinical remission of many months without the use of oral GCSs.</p>","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"50 1","pages":"105-108"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comments on: \"Long-term efficacy of mesenchymal stem cell treatment for complex perianal fistulas: A systematic review and meta-analysis\".","authors":"Lan Wang, Fang Cheng","doi":"10.5114/ceji.2025.149256","DOIUrl":"10.5114/ceji.2025.149256","url":null,"abstract":"<p><p>The meta-analysis by Wang et al. aimed to assess the long-term effects of mesenchymal stem cells on complex perianal fistula. The authors concluded that mesenchymal stem cell therapy has a long-term effect on the clinical response of complex perianal fistula and should be widely promoted not only in adults but also in infants and adolescents; however, more research on this topic is needed. We appreciate the authors' hard work, and we also agree with this argument. However, we have several concerns about the study. We think it is necessary to discuss the effect of anti-TNF and immunosuppressive therapy on the effcacy of mesenchymal stem cell treatment for perianal fistula in future trials, in order to optimize treatment strategies in perianal fistula patients and reduce the economic burden of patients. In the future, it will be interesting to assess the safety and feasibility of injection of fibrin glue combined with mesenchymal stem cells in perianal fistula.</p>","PeriodicalId":9694,"journal":{"name":"Central European Journal of Immunology","volume":"50 1","pages":"11-12"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}