Cell最新文献 - Book学术

Solute carriers: The gatekeepers of metabolism

IF 64.5 1区生物学

Cell Pub Date : 2025-02-20 DOI: 10.1016/j.cell.2025.01.015

Artem Khan, Yuyang Liu, Mark Gad, Timothy C. Kenny, Kıvanç Birsoy

引用次数: 0

Plasmodesmata act as unconventional membrane contact sites regulating intercellular molecular exchange in plants

IF 64.5 1区生物学

Cell Pub Date : 2025-02-20 DOI: 10.1016/j.cell.2024.11.034

Jessica Pérez-Sancho, Marija Smokvarska, Gwennogan Dubois, Marie Glavier, Sujith Sritharan, Tatiana S. Moraes, Hortense Moreau, Victor Dietrich, Matthieu P. Platre, Andrea Paterlini, Ziqiang P. Li, Laetitia Fouillen, Magali S. Grison, Pepe Cana-Quijada, Françoise Immel, Valerie Wattelet, Mathieu Ducros, Lysiane Brocard, Clément Chambaud, Yongming Luo, Emmanuelle M. Bayer

{"title":"Plasmodesmata act as unconventional membrane contact sites regulating intercellular molecular exchange in plants","authors":"Jessica Pérez-Sancho, Marija Smokvarska, Gwennogan Dubois, Marie Glavier, Sujith Sritharan, Tatiana S. Moraes, Hortense Moreau, Victor Dietrich, Matthieu P. Platre, Andrea Paterlini, Ziqiang P. Li, Laetitia Fouillen, Magali S. Grison, Pepe Cana-Quijada, Françoise Immel, Valerie Wattelet, Mathieu Ducros, Lysiane Brocard, Clément Chambaud, Yongming Luo, Emmanuelle M. Bayer","doi":"10.1016/j.cell.2024.11.034","DOIUrl":"https://doi.org/10.1016/j.cell.2024.11.034","url":null,"abstract":"Membrane contact sites (MCSs) are fundamental for intracellular communication, but their role in intercellular communication remains unexplored. We show that in plants, plasmodesmata communication bridges function as atypical endoplasmic reticulum (ER)-plasma membrane (PM) tubular MCSs, operating at cell-cell interfaces. Similar to other MCSs, ER-PM apposition is controlled by a protein-lipid tethering complex, but uniquely, this serves intercellular communication. Combining high-resolution microscopy, molecular dynamics, and pharmacological and genetic approaches, we show that cell-cell trafficking is modulated through the combined action of multiple C2 domains transmembrane domain proteins (MCTPs) 3, 4, and 6 ER-PM tethers and phosphatidylinositol-4-phosphate (PI4P) lipid. Graded PI4P amounts regulate MCTP docking to the PM, their plasmodesmata localization, and cell-cell permeability. SAC7, an ER-localized PI4P-phosphatase, regulates MCTP4 accumulation at plasmodesmata and modulates cell-cell trafficking capacity in a cell-type-specific manner. Our findings expand MCS functions in information transmission from intracellular to intercellular cellular activities.","PeriodicalId":9656,"journal":{"name":"Cell","volume":"29 1","pages":""},"PeriodicalIF":64.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A nucleosome switch primes hepatitis B virus infection

IF 64.5 1区生物学

Cell Pub Date : 2025-02-20 DOI: 10.1016/j.cell.2025.01.033

Nicholas A. Prescott, Tracy Biaco, Andrés Mansisidor, Yaron Bram, Justin Rendleman, Sarah C. Faulkner, Abigail A. Lemmon, Christine Lim, Rachel Tiersky, Eralda Salataj, Liliana Garcia-Martinez, Rodrigo L. Borges, Lluis Morey, Pierre-Jacques Hamard, Richard P. Koche, Viviana I. Risca, Robert E. Schwartz, Yael David

{"title":"A nucleosome switch primes hepatitis B virus infection","authors":"Nicholas A. Prescott, Tracy Biaco, Andrés Mansisidor, Yaron Bram, Justin Rendleman, Sarah C. Faulkner, Abigail A. Lemmon, Christine Lim, Rachel Tiersky, Eralda Salataj, Liliana Garcia-Martinez, Rodrigo L. Borges, Lluis Morey, Pierre-Jacques Hamard, Richard P. Koche, Viviana I. Risca, Robert E. Schwartz, Yael David","doi":"10.1016/j.cell.2025.01.033","DOIUrl":"https://doi.org/10.1016/j.cell.2025.01.033","url":null,"abstract":"Chronic hepatitis B virus (HBV) infection is an incurable pathogen responsible for causing liver disease and hepatocellular carcinoma. During the genesis of infection, HBV establishes an independent minichromosome consisting of the viral covalently closed circular DNA (cccDNA) genome and host histones. The viral X gene must be expressed immediately upon infection to induce degradation of the host silencing factor, the Smc5/6 complex. However, the relationship between cccDNA chromatinization and X gene transcription remains poorly understood. By establishing a reconstituted viral minichromosome platform, we found that nucleosome occupancy in cccDNA regulates X transcription. We corroborated these findings in situ and further showed that the chromatin-destabilizing molecule CBL137 inhibits full-length X transcription and HBV infection in primary human hepatocytes. Our results shed light on a long-standing paradox and represent a potential therapeutic approach for the treatment of chronic HBV infection.","PeriodicalId":9656,"journal":{"name":"Cell","volume":"20 1","pages":""},"PeriodicalIF":64.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular responses to RNA damage

IF 64.5 1区生物学

Cell Pub Date : 2025-02-20 DOI: 10.1016/j.cell.2025.01.005

Jacqueline Cordes, Shubo Zhao, Carla M. Engel, Julian Stingele

引用次数: 0

Advances in the study and treatment of genetic cardiomyopathies

IF 64.5 1区生物学

Cell Pub Date : 2025-02-20 DOI: 10.1016/j.cell.2025.01.011

Victoria N. Parikh, Sharlene M. Day, Neal K. Lakdawala, Eric D. Adler, Iacopo Olivotto, Christine E. Seidman, Carolyn Y. Ho

引用次数: 0

SecY translocon chaperones protein folding during membrane protein insertion

IF 64.5 1区生物学

Cell Pub Date : 2025-02-19 DOI: 10.1016/j.cell.2025.01.037

Xiaomin Ou, Chengying Ma, Dongjie Sun, Jinkun Xu, Yang Wang, Xiaofei Wu, Dali Wang, Song Yang, Ning Gao, Chen Song, Long Li

引用次数: 0

Contextual computation by competitive protein dimerization networks

IF 64.5 1区生物学

Cell Pub Date : 2025-02-19 DOI: 10.1016/j.cell.2025.01.036

Jacob Parres-Gold, Matthew Levine, Benjamin Emert, Andrew Stuart, Michael B. Elowitz

{"title":"Contextual computation by competitive protein dimerization networks","authors":"Jacob Parres-Gold, Matthew Levine, Benjamin Emert, Andrew Stuart, Michael B. Elowitz","doi":"10.1016/j.cell.2025.01.036","DOIUrl":"https://doi.org/10.1016/j.cell.2025.01.036","url":null,"abstract":"Many biological signaling pathways employ proteins that competitively dimerize in diverse combinations. These dimerization networks can perform biochemical computations in which the concentrations of monomer inputs determine the concentrations of dimer outputs. Despite their prevalence, little is known about the range of input-output computations that dimerization networks can perform and how it depends on network size and connectivity. Using a systematic computational approach, we demonstrate that even small dimerization networks of 3–6 monomers are expressive, performing diverse multi-input computations. Further, dimerization networks are versatile, performing different computations when their protein components are expressed at different levels, such as in different cell types. Remarkably, individual networks with random interaction affinities, when large enough, can perform nearly all potential one-input network computations merely by tuning their monomer expression levels. Thus, even the simple process of competitive dimerization provides a powerful architecture for multi-input, cell-type-specific signal processing.","PeriodicalId":9656,"journal":{"name":"Cell","volume":"35 1","pages":""},"PeriodicalIF":64.5,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143451839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Motor and vestibular signals in the visual cortex permit the separation of self versus externally generated visual motion

IF 64.5 1区生物学

Cell Pub Date : 2025-02-19 DOI: 10.1016/j.cell.2025.01.032

Mateo Vélez-Fort, Lee Cossell, Laura Porta, Claudia Clopath, Troy W. Margrie

引用次数: 0

Reprogrammable RNA-targeting CRISPR systems evolved from RNA toxin-antitoxins

IF 64.5 1区生物学

Cell Pub Date : 2025-02-18 DOI: 10.1016/j.cell.2025.01.034

Shai Zilberzwige-Tal, Han Altae-Tran, Soumya Kannan, Max E. Wilkinson, Samuel Chau-Duy-Tam Vo, Daniel Strebinger, KeHuan K. Edmonds, Chun-Chen Jerry Yao, Kepler S. Mears, Sergey A. Shmakov, Kira S. Makarova, Rhiannon K. Macrae, Eugene V. Koonin, Feng Zhang

{"title":"Reprogrammable RNA-targeting CRISPR systems evolved from RNA toxin-antitoxins","authors":"Shai Zilberzwige-Tal, Han Altae-Tran, Soumya Kannan, Max E. Wilkinson, Samuel Chau-Duy-Tam Vo, Daniel Strebinger, KeHuan K. Edmonds, Chun-Chen Jerry Yao, Kepler S. Mears, Sergey A. Shmakov, Kira S. Makarova, Rhiannon K. Macrae, Eugene V. Koonin, Feng Zhang","doi":"10.1016/j.cell.2025.01.034","DOIUrl":"https://doi.org/10.1016/j.cell.2025.01.034","url":null,"abstract":"Despite ongoing efforts to study CRISPR systems, the evolutionary origins giving rise to reprogrammable RNA-guided mechanisms remain poorly understood. Here, we describe an integrated sequence/structure evolutionary tracing approach to identify the ancestors of the RNA-targeting CRISPR-Cas13 system. We find that Cas13 likely evolved from AbiF, which is encoded by an abortive infection-linked gene that is stably associated with a conserved non-coding RNA (ncRNA). We further characterize a miniature Cas13, classified here as Cas13e, which serves as an evolutionary intermediate between AbiF and other known Cas13s. Despite this relationship, we show that their functions substantially differ. Whereas Cas13e is an RNA-guided RNA-targeting system, AbiF is a toxin-antitoxin (TA) system with an RNA antitoxin. We solve the structure of AbiF using cryoelectron microscopy (cryo-EM), revealing basic structural alterations that set Cas13s apart from AbiF. Finally, we map the key structural changes that enabled a non-guided TA system to evolve into an RNA-guided CRISPR system.","PeriodicalId":9656,"journal":{"name":"Cell","volume":"51 1","pages":""},"PeriodicalIF":64.5,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143435503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bat-infecting merbecovirus HKU5-CoV lineage 2 can use human ACE2 as a cell entry receptor

IF 64.5 1区生物学

Cell Pub Date : 2025-02-18 DOI: 10.1016/j.cell.2025.01.042

Jing Chen, Wei Zhang, Yang Li, Chen Liu, Tianyi Dong, Huiyu Chen, Chunguang Wu, Jia Su, Bei Li, Wei Zhang, Ben Hu, Jingkun Jia, Cheng-Bao Ma, Yan Zhu, Xiangyang He, Ang Li, Kaiyi Pan, Haofeng Lin, Zishuo Guo, Cong Li, Zheng-Li Shi

{"title":"Bat-infecting merbecovirus HKU5-CoV lineage 2 can use human ACE2 as a cell entry receptor","authors":"Jing Chen, Wei Zhang, Yang Li, Chen Liu, Tianyi Dong, Huiyu Chen, Chunguang Wu, Jia Su, Bei Li, Wei Zhang, Ben Hu, Jingkun Jia, Cheng-Bao Ma, Yan Zhu, Xiangyang He, Ang Li, Kaiyi Pan, Haofeng Lin, Zishuo Guo, Cong Li, Zheng-Li Shi","doi":"10.1016/j.cell.2025.01.042","DOIUrl":"https://doi.org/10.1016/j.cell.2025.01.042","url":null,"abstract":"Merbecoviruses comprise four viral species with remarkable genetic diversity: MERS-related coronavirus, Tylonycteris bat coronavirus HKU4, Pipistrellus bat coronavirus HKU5, and Hedgehog coronavirus 1. However, the potential human spillover risk of animal merbecoviruses remains to be investigated. Here, we reported the discovery of HKU5-CoV lineage 2 (HKU5-CoV-2) in bats that efficiently utilize human angiotensin-converting enzyme 2 (ACE2) as a functional receptor and exhibits a broad host tropism. Cryo-EM analysis of HKU5-CoV-2 receptor-binding domain (RBD) and human ACE2 complex revealed an entirely distinct binding mode compared with other ACE2-utilizing merbecoviruses with RBD footprint largely shared with ACE2-using sarbecoviruses and NL63. Structural and functional analyses indicate that HKU5-CoV-2 has a better adaptation to human ACE2 than lineage 1 HKU5-CoV. Authentic HKU5-CoV-2 infected human ACE2-expressing cell lines and human respiratory and enteric organoids. This study reveals a distinct lineage of HKU5-CoVs in bats that efficiently use human ACE2 and underscores their potential zoonotic risk.","PeriodicalId":9656,"journal":{"name":"Cell","volume":"31 1","pages":""},"PeriodicalIF":64.5,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143435502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0