Cell最新文献 - Book学术

Glia-like taste cells mediate an intercellular mode of peripheral sweet adaptation 类胶质味觉细胞介导外周甜味适应的细胞间模式

IF 64.5 1区生物学

Cell Pub Date : 2024-11-18 DOI: 10.1016/j.cell.2024.10.041

Gha Yeon Park, Geehyun Lee, Jongmin Yoon, Jisoo Han, Pyonggang Choi, Minjae Kim, Sungho Lee, Chaeri Park, Zhaofa Wu, Yulong Li, Myunghwan Choi

{"title":"Glia-like taste cells mediate an intercellular mode of peripheral sweet adaptation","authors":"Gha Yeon Park, Geehyun Lee, Jongmin Yoon, Jisoo Han, Pyonggang Choi, Minjae Kim, Sungho Lee, Chaeri Park, Zhaofa Wu, Yulong Li, Myunghwan Choi","doi":"10.1016/j.cell.2024.10.041","DOIUrl":"https://doi.org/10.1016/j.cell.2024.10.041","url":null,"abstract":"The sense of taste generally shows diminishing sensitivity to prolonged sweet stimuli, referred to as sweet adaptation. Yet, its mechanistic landscape remains incomplete. Here, we report that glia-like type I cells provide a distinct mode of sweet adaptation via intercellular crosstalk with chemosensory type II cells. Using the microfluidic-based intravital tongue imaging system, we found that sweet adaptation is facilitated along the synaptic transduction from type II cells to gustatory afferent nerves, while type I cells display temporally delayed and prolonged activities. We identified that type I cells receive purinergic input from adjacent type II cells via P2RY2 and provide inhibitory feedback to the synaptic transduction of sweet taste. Aligning with our cellular-level findings, purinergic activation of type I cells attenuated sweet licking behavior, and P2RY2 knockout mice showed decelerated adaptation behavior. Our study highlights a veiled intercellular mode of sweet adaptation, potentially contributing to the efficient encoding of prolonged sweetness.","PeriodicalId":9656,"journal":{"name":"Cell","volume":"21 1","pages":""},"PeriodicalIF":64.5,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Arabidopsis blue-light photoreceptor CRY2 is active in darkness to inhibit root growth 拟南芥蓝光光敏感受器 CRY2 在黑暗中具有抑制根系生长的活性

IF 64.5 1区生物学

Cell Pub Date : 2024-11-15 DOI: 10.1016/j.cell.2024.10.031

Desheng Zeng, Junqing Lv, Xu Li, Hongtao Liu

引用次数: 0

Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice 压力会破坏小鼠外侧杏仁核中的记忆组合，使威胁记忆泛化

IF 64.5 1区生物学

Cell Pub Date : 2024-11-15 DOI: 10.1016/j.cell.2024.10.034

Sylvie L. Lesuis, Sungmo Park, Annelies Hoorn, Asim J. Rashid, Andrew J. Mocle, Eric W. Salter, Stefan Vislavski, Madison T. Gray, Angelica M. Torelli, Antonietta DeCristofaro, Wouter P.F. Driever, Mario van der Stelt, Larry S. Zweifel, Graham L. Collingridge, Julie L. Lefebvre, Brandon J. Walters, Paul W. Frankland, Matthew N. Hill, Sheena A. Josselyn

{"title":"Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice","authors":"Sylvie L. Lesuis, Sungmo Park, Annelies Hoorn, Asim J. Rashid, Andrew J. Mocle, Eric W. Salter, Stefan Vislavski, Madison T. Gray, Angelica M. Torelli, Antonietta DeCristofaro, Wouter P.F. Driever, Mario van der Stelt, Larry S. Zweifel, Graham L. Collingridge, Julie L. Lefebvre, Brandon J. Walters, Paul W. Frankland, Matthew N. Hill, Sheena A. Josselyn","doi":"10.1016/j.cell.2024.10.034","DOIUrl":"https://doi.org/10.1016/j.cell.2024.10.034","url":null,"abstract":"Stress induces aversive memory overgeneralization, a hallmark of many psychiatric disorders. Memories are encoded by a sparse ensemble of neurons active during an event (an engram ensemble). We examined the molecular and circuit processes mediating stress-induced threat memory overgeneralization in mice. Stress, acting via corticosterone, increased the density of engram ensembles supporting a threat memory in lateral amygdala, and this engram ensemble was reactivated by both specific and non-specific retrieval cues (generalized threat memory). Furthermore, we identified a critical role for endocannabinoids, acting retrogradely on parvalbumin-positive (PV+) lateral amygdala interneurons in the formation of a less-sparse engram and memory generalization induced by stress. Glucocorticoid receptor antagonists, endocannabinoid synthesis inhibitors, increasing PV+ neuronal activity, and knocking down cannabinoid receptors in lateral amygdala PV+ neurons restored threat memory specificity and a sparse engram in stressed mice. These findings offer insights into stress-induced memory alterations, providing potential therapeutic avenues for stress-related disorders.","PeriodicalId":9656,"journal":{"name":"Cell","volume":"11 1","pages":""},"PeriodicalIF":64.5,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The single-molecule accessibility landscape of newly replicated mammalian chromatin 新复制的哺乳动物染色质的单分子可及性图谱

IF 64.5 1区生物学

Cell Pub Date : 2024-11-15 DOI: 10.1016/j.cell.2024.10.039

Megan S. Ostrowski, Marty G. Yang, Colin P. McNally, Nour J. Abdulhay, Simai Wang, Keerthi Renduchintala, Iryna Irkliyenko, Alva Biran, Brandon T.L. Chew, Ayush D. Midha, Emily V. Wong, Jonathan Sandoval, Isha H. Jain, Anja Groth, Elphège P. Nora, Hani Goodarzi, Vijay Ramani

{"title":"The single-molecule accessibility landscape of newly replicated mammalian chromatin","authors":"Megan S. Ostrowski, Marty G. Yang, Colin P. McNally, Nour J. Abdulhay, Simai Wang, Keerthi Renduchintala, Iryna Irkliyenko, Alva Biran, Brandon T.L. Chew, Ayush D. Midha, Emily V. Wong, Jonathan Sandoval, Isha H. Jain, Anja Groth, Elphège P. Nora, Hani Goodarzi, Vijay Ramani","doi":"10.1016/j.cell.2024.10.039","DOIUrl":"https://doi.org/10.1016/j.cell.2024.10.039","url":null,"abstract":"We present replication-aware single-molecule accessibility mapping (RASAM), a method to nondestructively measure replication status and protein-DNA interactions on chromatin genome-wide. Using RASAM, we uncover a genome-wide state of single-molecule “hyperaccessibility” post-replication that resolves over several hours. Combining RASAM with cellular models for rapid protein degradation, we demonstrate that histone chaperone CAF-1 reduces nascent chromatin accessibility by filling single-molecular “gaps” and generating closely spaced dinucleosomes on replicated DNA. At cis-regulatory elements, we observe unique modes by which nascent chromatin hyperaccessibility resolves: at CCCTC-binding factor (CTCF)-binding sites, CTCF and nucleosomes compete, reducing CTCF occupancy and motif accessibility post-replication; at active transcription start sites, high chromatin accessibility is maintained, implying rapid re-establishment of nucleosome-free regions. Our study introduces a new paradigm for studying replicated chromatin fiber organization. More broadly, we uncover a unique organization of newly replicated chromatin that must be reset by active processes, providing a substrate for epigenetic reprogramming.","PeriodicalId":9656,"journal":{"name":"Cell","volume":"7 1","pages":""},"PeriodicalIF":64.5,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional genomics of human skeletal development and the patterning of height heritability 人类骨骼发育的功能基因组学与身高遗传的模式化

IF 64.5 1区生物学

Cell Pub Date : 2024-11-15 DOI: 10.1016/j.cell.2024.10.040

Daniel Richard, Pushpanathan Muthuirulan, Mariel Young, Loic Yengo, Sailaja Vedantam, Eirini Marouli, Eric Bartell, Joel Hirschhorn, Terence D. Capellini

{"title":"Functional genomics of human skeletal development and the patterning of height heritability","authors":"Daniel Richard, Pushpanathan Muthuirulan, Mariel Young, Loic Yengo, Sailaja Vedantam, Eirini Marouli, Eric Bartell, Joel Hirschhorn, Terence D. Capellini","doi":"10.1016/j.cell.2024.10.040","DOIUrl":"https://doi.org/10.1016/j.cell.2024.10.040","url":null,"abstract":"Underlying variation in height are regulatory changes to chondrocytes, cartilage cells comprising long-bone growth plates. Currently, we lack knowledge on epigenetic regulation and gene expression of chondrocytes sampled across the human skeleton, and therefore we cannot understand basic regulatory mechanisms controlling height biology. We first rectify this issue by generating extensive epigenetic and transcriptomic maps from chondrocytes sampled from different growth plates across developing human skeletons, discovering novel regulatory networks shaping human bone/joint development. Next, using these maps in tandem with height genome-wide association study (GWAS) signals, we disentangle the regulatory impacts that skeletal element-specific versus global-acting variants have on skeletal growth, revealing the prime importance of regulatory pleiotropy in controlling height variation. Finally, as height is highly heritable, and thus often the test case for complex-trait genetics, we leverage these datasets within a testable omnigenic model framework to discover novel chondrocyte developmental modules and peripheral-acting factors shaping height biology and skeletal growth.","PeriodicalId":9656,"journal":{"name":"Cell","volume":"98 1","pages":""},"PeriodicalIF":64.5,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic manipulation of Patescibacteria provides mechanistic insights into microbial dark matter and the epibiotic lifestyle. Patescibacteria的基因操作为微生物暗物质和表生生物的生活方式提供了机制上的见解。

IF 45.5 1区生物学

Cell Pub Date : 2023-10-26 Epub Date: 2023-09-07 DOI: 10.1016/j.cell.2023.08.017

Yaxi Wang, Larry A Gallagher, Pia A Andrade, Andi Liu, Ian R Humphreys, Serdar Turkarslan, Kevin J Cutler, Mario L Arrieta-Ortiz, Yaqiao Li, Matthew C Radey, Jeffrey S McLean, Qian Cong, David Baker, Nitin S Baliga, S Brook Peterson, Joseph D Mougous

{"title":"Genetic manipulation of Patescibacteria provides mechanistic insights into microbial dark matter and the epibiotic lifestyle.","authors":"Yaxi Wang, Larry A Gallagher, Pia A Andrade, Andi Liu, Ian R Humphreys, Serdar Turkarslan, Kevin J Cutler, Mario L Arrieta-Ortiz, Yaqiao Li, Matthew C Radey, Jeffrey S McLean, Qian Cong, David Baker, Nitin S Baliga, S Brook Peterson, Joseph D Mougous","doi":"10.1016/j.cell.2023.08.017","DOIUrl":"10.1016/j.cell.2023.08.017","url":null,"abstract":"Patescibacteria, also known as the candidate phyla radiation (CPR), are a diverse group of bacteria that constitute a disproportionately large fraction of microbial dark matter. Its few cultivated members, belonging mostly to Saccharibacteria, grow as epibionts on host Actinobacteria. Due to a lack of suitable tools, the genetic basis of this lifestyle and other unique features of Patescibacteira remain unexplored. Here, we show that Saccharibacteria exhibit natural competence, and we exploit this property for their genetic manipulation. Imaging of fluorescent protein-labeled Saccharibacteria provides high spatiotemporal resolution of phenomena accompanying epibiotic growth, and a transposon-insertion sequencing (Tn-seq) genome-wide screen reveals the contribution of enigmatic Saccharibacterial genes to growth on their hosts. Finally, we leverage metagenomic data to provide cutting-edge protein structure-based bioinformatic resources that support the strain Southlakia epibionticum and its corresponding host, Actinomyces israelii, as a model system for unlocking the molecular underpinnings of the epibiotic lifestyle.","PeriodicalId":9656,"journal":{"name":"Cell","volume":" ","pages":"4803-4817.e13"},"PeriodicalIF":45.5,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10633639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10188842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormonal gatekeeping via the blood-brain barrier governs caste-specific behavior in ants. 通过血脑屏障的激素门控控制蚂蚁的种姓特定行为。

IF 45.5 1区生物学

Cell Pub Date : 2023-09-28 Epub Date: 2023-09-07 DOI: 10.1016/j.cell.2023.08.002

Linyang Ju, Karl M Glastad, Lihong Sheng, Janko Gospocic, Callum J Kingwell, Shawn M Davidson, Sarah D Kocher, Roberto Bonasio, Shelley L Berger

引用次数: 0

An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development. 免疫细胞图谱揭示了人类巨噬细胞在产前发育过程中的动态特性。

IF 64.5 1区生物学

Cell Pub Date : 2023-09-28 Epub Date: 2023-09-12 DOI: 10.1016/j.cell.2023.08.019

Zeshuai Wang, Zhisheng Wu, Hao Wang, Ruoqing Feng, Guanlin Wang, Muxi Li, Shuang-Yin Wang, Xiaoyan Chen, Yiyi Su, Jun Wang, Weiwen Zhang, Yuzhou Bao, Zhenwei Lan, Zhuo Song, Yiheng Wang, Xianyang Luo, Lingyu Zhao, Anli Hou, Shuye Tian, Hongliang Gao, Wenbin Miao, Yingyu Liu, Huilin Wang, Cui Yin, Zhi-Liang Ji, Mingqian Feng, Hongkun Liu, Lianghui Diao, Ido Amit, Yun Chen, Yong Zeng, Florent Ginhoux, Xueqing Wu, Yuanfang Zhu, Hanjie Li

{"title":"An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development.","authors":"Zeshuai Wang, Zhisheng Wu, Hao Wang, Ruoqing Feng, Guanlin Wang, Muxi Li, Shuang-Yin Wang, Xiaoyan Chen, Yiyi Su, Jun Wang, Weiwen Zhang, Yuzhou Bao, Zhenwei Lan, Zhuo Song, Yiheng Wang, Xianyang Luo, Lingyu Zhao, Anli Hou, Shuye Tian, Hongliang Gao, Wenbin Miao, Yingyu Liu, Huilin Wang, Cui Yin, Zhi-Liang Ji, Mingqian Feng, Hongkun Liu, Lianghui Diao, Ido Amit, Yun Chen, Yong Zeng, Florent Ginhoux, Xueqing Wu, Yuanfang Zhu, Hanjie Li","doi":"10.1016/j.cell.2023.08.019","DOIUrl":"10.1016/j.cell.2023.08.019","url":null,"abstract":"Macrophages are heterogeneous and play critical roles in development and disease, but their diversity, function, and specification remain inadequately understood during human development. We generated a single-cell RNA sequencing map of the dynamics of human macrophage specification from PCW 4-26 across 19 tissues. We identified a microglia-like population and a proangiogenic population in 15 macrophage subtypes. Microglia-like cells, molecularly and morphologically similar to microglia in the CNS, are present in the fetal epidermis, testicle, and heart. They are the major immune population in the early epidermis, exhibit a polarized distribution along the dorsal-lateral-ventral axis, and interact with neural crest cells, modulating their differentiation along the melanocyte lineage. Through spatial and differentiation trajectory analysis, we also showed that proangiogenic macrophages are perivascular across fetal organs and likely yolk-sac-derived as microglia. Our study provides a comprehensive map of the heterogeneity and developmental dynamics of human macrophages and unravels their diverse functions during development.","PeriodicalId":9656,"journal":{"name":"Cell","volume":" ","pages":"4454-4471.e19"},"PeriodicalIF":64.5,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10597298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal insight into early pregnancy governed by immune-featured stromal cells. 免疫基质细胞对早孕的时空洞察。

IF 64.5 1区生物学

Cell Pub Date : 2023-09-28 Epub Date: 2023-09-11 DOI: 10.1016/j.cell.2023.08.020

Min Yang, Jennie Ong, Fanju Meng, Feixiang Zhang, Hui Shen, Kerstin Kitt, Tengfei Liu, Wei Tao, Peng Du

{"title":"Spatiotemporal insight into early pregnancy governed by immune-featured stromal cells.","authors":"Min Yang, Jennie Ong, Fanju Meng, Feixiang Zhang, Hui Shen, Kerstin Kitt, Tengfei Liu, Wei Tao, Peng Du","doi":"10.1016/j.cell.2023.08.020","DOIUrl":"10.1016/j.cell.2023.08.020","url":null,"abstract":"Endometrial decidualization connecting embryo implantation and placentation is transient but essential for successful pregnancy, which, however, is not systematically investigated. Here, we use a scStereo-seq technology to spatially visualize and define the dynamic functional decidual hubs assembled by distinct immune, endothelial, trophoblast, and decidual stromal cells (DSCs) in early pregnant mice. We unravel the DSC transdifferentiation trajectory and surprisingly discover a dual-featured type of immune-featured DSCs (iDSCs). We find that immature DSCs attract immune cells and induce decidual angiogenesis at the mesenchymal-epithelial transition hub during decidualization initiation. iDSCs enable immune cell recruitment and suppression, govern vascularization, and promote cytolysis at immune cell assembling and vascular hubs, respectively, to establish decidual homeostasis at a later stage. Interestingly, dysfunctional and spatially disordered iDSCs cause abnormal accumulation of immune cells in the vascular hub, which disrupts decidual hub specification and eventually leads to pregnancy complications in DBA/2-mated CBA/J mice.","PeriodicalId":9656,"journal":{"name":"Cell","volume":" ","pages":"4271-4288.e24"},"PeriodicalIF":64.5,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10220608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries. 信使核糖核酸拷贝的自分层缓冲信使核糖核酸：信使核糖核酸和信使核糖核酸调节因子的化学计量。

IF 64.5 1区生物学

Cell Pub Date : 2023-09-28 Epub Date: 2023-09-12 DOI: 10.1016/j.cell.2023.08.018

Andrés H Cardona, Szilvia Ecsedi, Mokrane Khier, Zhou Yi, Alia Bahri, Amira Ouertani, Florian Valero, Margaux Labrosse, Sami Rouquet, Stéphane Robert, Agnès Loubat, Danielle Adekunle, Arnaud Hubstenberger

{"title":"Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries.","authors":"Andrés H Cardona, Szilvia Ecsedi, Mokrane Khier, Zhou Yi, Alia Bahri, Amira Ouertani, Florian Valero, Margaux Labrosse, Sami Rouquet, Stéphane Robert, Agnès Loubat, Danielle Adekunle, Arnaud Hubstenberger","doi":"10.1016/j.cell.2023.08.018","DOIUrl":"10.1016/j.cell.2023.08.018","url":null,"abstract":"Cellular homeostasis requires the robust control of biomolecule concentrations, but how do millions of mRNAs coordinate their stoichiometries in the face of dynamic translational changes? Here, we identified a two-tiered mechanism controlling mRNA:mRNA and mRNA:protein stoichiometries where mRNAs super-assemble into condensates with buffering capacity and sorting selectivity through phase-transition mechanisms. Using C. elegans oogenesis arrest as a model, we investigated the transcriptome cytosolic reorganization through the sequencing of RNA super-assemblies coupled with single mRNA imaging. Tightly repressed mRNAs self-assembled into same-sequence nanoclusters that further co-assembled into multiphase condensates. mRNA self-sorting was concentration dependent, providing a self-buffering mechanism that is selective to sequence identity and controls mRNA:mRNA stoichiometries. The cooperative sharing of limiting translation repressors between clustered mRNAs prevented the disruption of mRNA:repressor stoichiometries in the cytosol. Robust control of mRNA:mRNA and mRNA:protein stoichiometries emerges from mRNA self-demixing and cooperative super-assembly into multiphase multiscale condensates with dynamic storage capacity.","PeriodicalId":9656,"journal":{"name":"Cell","volume":" ","pages":"4310-4324.e23"},"PeriodicalIF":64.5,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10597299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0