Natural Product Reports最新文献_第9页

Chemical language models for natural product discovery. 天然产物发现的化学语言模型。

IF 10.6 1区化学

Natural Product Reports Pub Date : 2026-05-12 DOI: 10.1039/d6np00002a

Koh Sakano, Kairi Furui, Apakorn Kengkanna, Yuta Kikuchi, Masahito Ohue

引用次数: 0

Structural determination of natural products at speeds and scale of high-throughput screening. 天然产物的结构测定在高通量筛选的速度和规模。

IF 12.5 1区化学

Natural Product Reports Pub Date : 2026-05-11 DOI: 10.1039/d6np00016a

Christopher C Thornburg, Rohitesh Kumar, Dongdong Wang, Barry R O'Keefe, Tanja Grkovic

引用次数: 0

High-throughput assays for SAM-dependent methyltransferases: advances, challenges, and future perspectives. sam依赖性甲基转移酶的高通量测定：进展、挑战和未来展望

IF 10.6 1区化学

Natural Product Reports Pub Date : 2026-05-05 DOI: 10.1039/d6np00006a

Renia Fotiadou, Florian Heinz, Konstantin F G Weigmann, Mark Doerr, Uwe T Bornscheuer, Ioannis V Pavlidis

{"title":"High-throughput assays for SAM-dependent methyltransferases: advances, challenges, and future perspectives.","authors":"Renia Fotiadou, Florian Heinz, Konstantin F G Weigmann, Mark Doerr, Uwe T Bornscheuer, Ioannis V Pavlidis","doi":"10.1039/d6np00006a","DOIUrl":"https://doi.org/10.1039/d6np00006a","url":null,"abstract":"Covering: up to January 2026Methyltransferases (EC 2.1.1.-) represent one of the most functionally diverse enzyme families in biology. Found across all domains of life-including bacteria, archaea, eukaryotes, and viruses-these enzymes catalyze a wide range of methylation reactions, commonly on O-, N-, and C-atoms, in a chemo- and regioselective manner. The wide set of substrates methylated is why a universal assay for the methyltransferase's activity screening has not yet been established. Despite this diversity, most methyltransferases utilize a common methyl donor, the S-adenosyl-L-methionine (SAM). This shared feature has enabled the development of several assay strategies based on the detection of S-adenosyl-L-homocysteine, the by-product of SAM-dependent methylation, or its degradation products. Colorimetric and fluorometric assays have been widely employed to monitor methyltransferase activity, each with distinct advantages and limitations, depending on the substrate/product detection of the methylation reaction, but also whether they require purified enzymes or work in crude (E. coli) cell lysates. However, the methylation of substrates with more than one nucleophilic atom requires sophisticated analytical tools for accurate monitoring of the regiospecificity of methyltransferases. This complexity often limits the broader exploitation of methyltransferases, particularly in enzyme engineering efforts, where large datasets are generated and must be efficiently analyzed. This review critically examines the high-throughput screening assays developed to date for methyltransferase activity.","PeriodicalId":94,"journal":{"name":"Natural Product Reports","volume":" ","pages":""},"PeriodicalIF":10.6,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147830904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hot off the Press 刚出版的。

IF 10.6 1区化学

Natural Product Reports Pub Date : 2026-04-16 DOI: 10.1039/D6NP90014C

Robert A. Hill and Andrew Sutherland

引用次数: 0

Glucose 6-phosphate: the diversity of C-methylation in sugar moieties within natural product biosynthesis. 葡萄糖6-磷酸：天然产物生物合成中糖部分c -甲基化的多样性。

IF 10.6 1区化学

Natural Product Reports Pub Date : 2026-03-16 DOI: 10.1039/d5np00071h

Ziruo Zou, Michael Müller

{"title":"Glucose 6-phosphate: the diversity of C-methylation in sugar moieties within natural product biosynthesis.","authors":"Ziruo Zou, Michael Müller","doi":"10.1039/d5np00071h","DOIUrl":"https://doi.org/10.1039/d5np00071h","url":null,"abstract":"Covering: Up to 2026Methylation is one of the most frequent and functionally significant modifications in natural product biosynthesis. This transformation generally involves the cosubstrate S-adenosyl-L-methionine (SAM) and occurs with high regio-, chemo-, and stereo-selectivity. C-Methylation serves as an effective strategy for the incorporation of C1 building blocks and plays a key role in both carbon skeleton extension and structural core decoration. A notable example is SAM-dependent C-methylation of nucleotide-activated sugar moieties catalyzed by C-methyltransferase (C-MT) during the biosynthesis of glycosylated natural products. A prerequisite in these pathways is the activation of the sugar by the attachment of a nucleotide diphosphate (NDP) tail. Sugar C-MTs exhibit remarkable substrate specificity and often act in concert with additional transformations, such as oxidation, decarboxylation, or reduction, to diversify sugar structures. Canonical SAM-dependent sugar C-MTs are capable of introducing methyl groups at C3, C4, or C5 positions of the sugar ring by an SN2-like mechanism involving enolate intermediates. In contrast, C6 methylation has been attributed to radical SAM enzymes. These distinct strategies illustrate the enzymatic versatility and sophisticated control over sugar C-methylation in nature, contributing to the structural diversity of natural products.","PeriodicalId":94,"journal":{"name":"Natural Product Reports","volume":" ","pages":""},"PeriodicalIF":10.6,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147466278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brief overview of recently reported misassigned natural products and their in silico revisions enabled by DU8ML, a machine learning-augmented DFT computational NMR method. 简要概述了最近报道的错配天然产物及其通过DU8ML（一种机器学习增强的DFT计算核磁共振方法）实现的计算机修订。

IF 10.6 1区化学

Natural Product Reports Pub Date : 2026-03-10 DOI: 10.1039/d5np00088b

Srinivas Beduru, Andrei G Kutateladze

引用次数: 0

Synthetic biology for heterologous expression and engineering of fungal polyketide synthases 真菌聚酮合酶的异源表达与工程合成生物学。

IF 10.6 1区化学

Natural Product Reports Pub Date : 2026-03-02 DOI: 10.1039/D5NP00020C

Sidharth Jayachandran, Adrian Enrique Gadar-Lopez, Ling Chen, Kasper Krunderup Jakobsen, Coco Xie, Therese Horch, Jay D. Keasling and Pablo Cruz-Morales

{"title":"Synthetic biology for heterologous expression and engineering of fungal polyketide synthases","authors":"Sidharth Jayachandran, Adrian Enrique Gadar-Lopez, Ling Chen, Kasper Krunderup Jakobsen, Coco Xie, Therese Horch, Jay D. Keasling and Pablo Cruz-Morales","doi":"10.1039/D5NP00020C","DOIUrl":"10.1039/D5NP00020C","url":null,"abstract":"Covering: up to 2025Polyketide synthases (PKSs) are essential enzymatic systems that produce a wide range of natural products. Their biosynthetic features offer significant opportunities to create a broad range of polyketides for pharmaceutical, agricultural, and biotechnology applications. In this highlight article, we provide a brief overview of key aspects of PKS biosynthesis, their classification in bacteria and fungi, and the evolutionary mechanisms driving their diversification. After outlining the fundamentals of PKSs, we discuss two key design principles for fungal PKS engineering: host selection and engineering strategies. For host selection, we revisit the state of the art in heterologous expression in yeast and filamentous fungi. We then explore strategies for modifying fungal PKS activity through starter unit selection, point mutations, and domain swaps. Finally, we offer perspectives on future directions for fungal PKS engineering, highlighting the importance of exploring new hosts and integrating advanced computational tools.","PeriodicalId":94,"journal":{"name":"Natural Product Reports","volume":" 3","pages":" 401-414"},"PeriodicalIF":10.6,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147324004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial: Microbial host and metabolic engineering for natural product discovery and production 编辑：微生物宿主和代谢工程用于天然产物的发现和生产。

IF 10.6 1区化学

Natural Product Reports Pub Date : 2026-02-16 DOI: 10.1039/D6NP90006B

Alessandra S. Eustaquio, Eriko Takano and Tobias A. M. Gulder

引用次数: 0

Engineering microbiomes for natural product discovery and production 用于天然产物发现和生产的工程微生物组。

IF 10.6 1区化学

Natural Product Reports Pub Date : 2026-02-09 Epub Date: 2025-09-24 DOI: 10.1039/d5np00038f

Francesco Del Carratore , Rainer Breitling

{"title":"Engineering microbiomes for natural product discovery and production","authors":"Francesco Del Carratore , Rainer Breitling","doi":"10.1039/d5np00038f","DOIUrl":"10.1039/d5np00038f","url":null,"abstract":"<div><div>Covering: 2021 to 2025</div></div><div><div>Microbial communities represent a vast and largely untapped source of natural products with potential applications in various fields, including medicine, agriculture, and the biomanufacturing industry. Secondary metabolites play a crucial role in mediating interspecies interactions within these communities, influencing their structure and function. Recent advances in microbial genetic engineering and multi-omics technologies have enabled the harnessing of these interactions for enhanced natural product discovery and production. These techniques, coupled with systems biology and mathematical modelling, allow for the rational design and manipulation of microbial consortia to elicit the expression of cryptic biosynthetic gene clusters and to optimize the production of desired compounds. Additionally, direct mining of microbiomes using metagenomics, metatranscriptomics, and metabolomics has revealed a wealth of novel biosynthetic gene clusters and secondary metabolites with potential therapeutic and industrial value. Despite the challenges associated with cultivating and characterizing diverse microbial species, ongoing advancements in computational tools and data analysis are rapidly expanding our ability to explore and exploit the seemingly inexhaustible reservoir of natural products hidden within microbial communities.</div></div>","PeriodicalId":94,"journal":{"name":"Natural Product Reports","volume":"43 2","pages":"Pages 301-312"},"PeriodicalIF":10.6,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green genes from blue greens: challenges and solutions to unlocking the potential of cyanobacteria in drug discovery 来自蓝色绿色的绿色基因：挑战和解决方案，以解锁药物发现蓝藻的潜力。

IF 10.6 1区化学

Natural Product Reports Pub Date : 2026-02-09 Epub Date: 2025-07-15 DOI: 10.1039/d5np00016e

Benjamin Philmus , Nicole E. Avalon , Yousong Ding , Drew T. Doering , Alessandra S. Eustáquio , William H. Gerwick , Hendrik Luesch , Jimmy Orjala , Shaz Sutherland , Arnaud Taton , Daniel Udwary

引用次数: 0