Natural Product Reports最新文献_第7页

SAM-dependent methyltransferases: from chemoenzymatic natural product synthesis to industrial application. sam依赖的甲基转移酶：从化学酶天然产物合成到工业应用。

IF 12.5 1区化学

Natural Product Reports Pub Date : 2026-07-01 DOI: 10.1039/d6np00042h

Lisa M Böhmer, Benjamin P Chapple, Diana A Amariei, Martin A Hayes, Jörg Pietruszka

{"title":"SAM-dependent methyltransferases: from chemoenzymatic natural product synthesis to industrial application.","authors":"Lisa M Böhmer, Benjamin P Chapple, Diana A Amariei, Martin A Hayes, Jörg Pietruszka","doi":"10.1039/d6np00042h","DOIUrl":"https://doi.org/10.1039/d6np00042h","url":null,"abstract":"<p><p>Covering: up to 2026Over the past decades, research on <i>S</i>-adenosyl-L-methionine (SAM)-dependent methyltransferases (MTs) has led to their emergence as highly selective biocatalysts for late-stage methylation reactions, serving as new tools within the chemoenzymatic synthesis of complex natural products and chemical scaffolds. Studies focussing on the identification of novel MTs, their engineering and process optimisation, have not only expanded the accessible chemical space for biocatalytic methylations but have also significantly improved their applicability for reactions conducted at preparative scale. This includes innovations in enzyme-coupled SAM recycling systems and SAM analogue generation which have additionally enabled alkylation beyond methyl transfer. Structure-guided MT mutagenesis campaigns, together with the development of novel activity assays, have contributed to the expansion of the catalytic repertoire of MTs, thereby unlocking new reactivities. This highlight article provides a brief overview of recent developments in MT biocatalysis, with particular focus on their application in the chemoenzymatic synthesis of natural products at preparative scale. Current limitations associated with MT biocatalysis are examined, and future opportunities for the implementation of MT-catalysed transformations in industrial applications are discussed.</p>","PeriodicalId":94,"journal":{"name":"Natural Product Reports","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148358342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivar to chemotype: characterizing complex botanicals with mass spectrometry metabolomics 品种到化学型：用质谱代谢组学表征复杂植物。

IF 12.5 1区化学

Natural Product Reports Pub Date : 2026-06-26 Epub Date: 2025-12-03 DOI: 10.1039/d5np00040h

Joshua J. Kellogg , R. Teal Jordan , Madhusha M. Ranaweera , Kelsey Custer , Savannah G. Anez , Julia Bendlin , Francisco T. Chacon , Xiaoling Chen

{"title":"Cultivar to chemotype: characterizing complex botanicals with mass spectrometry metabolomics","authors":"Joshua J. Kellogg , R. Teal Jordan , Madhusha M. Ranaweera , Kelsey Custer , Savannah G. Anez , Julia Bendlin , Francisco T. Chacon , Xiaoling Chen","doi":"10.1039/d5np00040h","DOIUrl":"10.1039/d5np00040h","url":null,"abstract":"<div><div>Covering up to 2025</div></div><div><div>Plant products, including botanical dietary supplements, nutraceuticals, and herbal medicines, remain central to supporting human health and wellness. Their usage has been steadily increasing over the last few decades, which has also led to raised concerns about proper identification and characterization of plant materials. This information is crucial to evaluate the safety and efficacy of these botanical products and prevent misidentification or adulteration. While there are multiple analytical approaches to characterize botanicals, this review provides insight into how untargeted mass spectrometry metabolomics can profile these commonly complex mixtures and provide detailed datasets that are capable of taxonomically classifying samples, detecting adulteration, and providing insight into variation between plant materials and their nutritional, medicinal, or toxicological effects. We describe data analysis approaches for untargeted metabolomics, case studies on the various applications of this method for characterizing botanicals, and challenges that the growing field of mass spectrometry-based metabolomics is facing. The chosen topics reflect the current state of metabolomics analyses for complex systems with a look to the future of how to conceptualize botanical characterization.</div></div>","PeriodicalId":94,"journal":{"name":"Natural Product Reports","volume":"43 6","pages":"Pages 1181-1200"},"PeriodicalIF":12.5,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145675859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass spectrometry-based metabolomics approaches to interrogate host–microbiome interactions in mammalian systems 基于质谱的代谢组学方法在哺乳动物系统中询问宿主-微生物组相互作用。

IF 12.5 1区化学

Natural Product Reports Pub Date : 2026-06-26 Epub Date: 2025-06-16 DOI: 10.1039/d5np00021a

Atharva S. Kulkarni , Guilherme M. P. Carrara , Jiangpeiyun Jin , Jarrod Laro , Thilini Peramuna , Laura-Isobel McCall , Neha Garg

{"title":"Mass spectrometry-based metabolomics approaches to interrogate host–microbiome interactions in mammalian systems","authors":"Atharva S. Kulkarni , Guilherme M. P. Carrara , Jiangpeiyun Jin , Jarrod Laro , Thilini Peramuna , Laura-Isobel McCall , Neha Garg","doi":"10.1039/d5np00021a","DOIUrl":"10.1039/d5np00021a","url":null,"abstract":"<div><div>Covering: 2015 to 2025</div></div><div><div>Chemical crosstalk is universal to all life, niche-specific, and essential to thrive. This crosstalk is mediated by a large diversity of molecules, including metal ions, small molecules, polysaccharides, nucleic acids, lipids, and proteins. Among these, specialized small molecules referred to as natural products (NPs) play an important role in microbe–drug/environment interactions, microbe–microbe, and microbe–host interactions. Microbial communication using NPs allows microbes to sense quorum, form biofilms, eliminate competition, establish symbiosis, evade immune attack, and respond to stress. In most cases, the elucidation of small molecule mediators and effectors of microbe–host interactions presents a major challenge due to the relatively low abundance of microbial metabolites in a milieu of host, microbe, and environmental metabolites. Advances in analytical instrumentation, such as mass spectrometers, and both experimental as well as computational methods to analyze data, coupled with the use of model organisms, have enabled fundamental discoveries of mechanisms of small molecule-mediated host–microbe interactions. The focus of this review is to detail the approaches applied in the last decade to disentangle microbiome-derived NPs in human and murine model systems. Select recent findings from diverse biological ecosystems are discussed to inform relevant parallels and potential strategies for research in human health.</div></div>","PeriodicalId":94,"journal":{"name":"Natural Product Reports","volume":"43 6","pages":"Pages 1078-1105"},"PeriodicalIF":12.5,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolism and bioavailability aspects of natural products of plant origin using mass spectrometry-based and metabolomic approaches 利用质谱和代谢组学方法研究植物源天然产物的代谢和生物利用度。

IF 12.5 1区化学

Natural Product Reports Pub Date : 2026-06-26 Epub Date: 2025-10-28 DOI: 10.1039/d5np00022j

Eleni V. Mikropoulou , Aikaterini Basdeki , Maria Halabalaki

{"title":"Metabolism and bioavailability aspects of natural products of plant origin using mass spectrometry-based and metabolomic approaches","authors":"Eleni V. Mikropoulou , Aikaterini Basdeki , Maria Halabalaki","doi":"10.1039/d5np00022j","DOIUrl":"10.1039/d5np00022j","url":null,"abstract":"<div><div>Covering: 1982 to 2025</div></div><div><div>The therapeutic value of natural products (NPs) is well established, as evidenced by their rich ethnopharmacological history and the significant proportion of marketed drugs derived from natural sources. Despite their notable advantages such as structural versatility and scaffold diversity, NPs have been increasingly sidelined by the pharmaceutical industry due to the labor-intensive nature of their isolation and structural elucidation as well as issues related to patenting, sustainable sourcing and preclinical evaluation. Moreover, current bioavailability research focuses predominantly on well-known medicinal and edible plants or specific compound classes, leaving many other promising candidates underexplored. The interplay between the gut microbiota and NPs, which is critical for pharmacokinetics and ADME (absorption, distribution, metabolism, and excretion), is also overlooked. Numerous <em>in vitro</em> and <em>in vivo</em> models have been developed to study the ADME properties of xenobiotics, while human clinical trials remain scarce in the field of NPs. Recent technological advancements, including innovations in mass spectrometry (MS), smart library screening, dereplication, molecular networking, and metabolomics, have significantly improved the NP research pipeline, offering faster and more accurate compound identification. High-resolution instruments like Orbitrap, QTOF, FT-ICR, and MRMS, alongside IMS and advanced data acquisition techniques (DDA and DIA), now offer deeper insights into complex mixtures. Despite MS being a cornerstone of pharmacokinetics–pharmacodynamics (PK/PD) studies, the integration of metabolomics and big data analytics remains underutilized, particularly in NP prioritization. This review aims to explore the evolution of MS in NP metabolism studies, from early investigations to current multidisciplinary approaches, proposing a critical reflection on the challenges in NP drug development.</div></div>","PeriodicalId":94,"journal":{"name":"Natural Product Reports","volume":"43 6","pages":"Pages 1130-1180"},"PeriodicalIF":12.5,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145375535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hot off the Press 刚出版的。

IF 12.5 1区化学

Natural Product Reports Pub Date : 2026-06-26 Epub Date: 2026-06-10 DOI: 10.1039/d6np90022d

Robert A. Hill , Andrew Sutherland

引用次数: 0

Genomic scale analysis of assembly-line polyketide synthase diversity and evolution. 装配线聚酮合成酶多样性和进化的基因组尺度分析。

IF 12.5 1区化学

Natural Product Reports Pub Date : 2026-06-26 DOI: 10.1039/d6np00052e

Seokyoung Lee, Chaitan Khosla

{"title":"Genomic scale analysis of assembly-line polyketide synthase diversity and evolution.","authors":"Seokyoung Lee, Chaitan Khosla","doi":"10.1039/d6np00052e","DOIUrl":"https://doi.org/10.1039/d6np00052e","url":null,"abstract":"<p><p>Covering: up to 2026Assembly-line polyketide synthases (PKSs) are among the most sophisticated catalysts in nature, responsible for the biosynthesis of many medicinally important natural products including antibiotics, anticancer agents, immunosuppressants and veterinary agents. While conventional methods for polyketide natural product discovery have fallen out of favor, the rapid expansion of microbial genome sequencing has revealed a vast untapped diversity of assembly-line PKSs, most of which can be regarded as \"orphans\" in that their product identity is unknown. A clear picture of how this sequence diversity reflects the evolutionary history of assembly-line PKSs could enable judicious prioritization of experimental efforts aimed at decoding these orphans. To this end we have used a scalable curation workflow to hand-curate an updated catalogue, PKSClusterDB, that includes 16 633 non-redundant assembly-line PKSs. We have also formulated an automatable \"anchor-window\" framework based on conserved multimodular segments of assembly-line PKSs to identify and interrogate PKS families of interest. Application of this framework to three different PKS families extracted from PKSClusterDB revealed lineage-dependent patterns of diversification, ranging from broadly diversified families to more compact or sharply bounded lineages. PKSClusterDB has also proven useful in exploring other aspects of PKS diversity, including scaffold architecture, extender-unit specificity, reductive-state programming, stereochemical control and modular organization. In closing, we consider how advances in machine learning could be harnessed to accelerate our understanding of assembly-line PKS evolution, diversity and biosynthetic mechanisms.</p>","PeriodicalId":94,"journal":{"name":"Natural Product Reports","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148336968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scalability of mass spectrometry-based metabolomics for natural extracts libraries exploration: current status, challenges, and opportunities 基于质谱的代谢组学在天然提取物文库探索中的可扩展性：现状、挑战和机遇。

IF 12.5 1区化学

Natural Product Reports Pub Date : 2026-06-26 Epub Date: 2025-09-15 DOI: 10.1039/d5np00034c

Adriano Rutz , Wout Bittremieux , Robin Schmid , Olivier Cailloux , Justin J. J. van der Hooft , Mehdi A. Beniddir

引用次数: 0

Mass spectrometry as the unifying force in natural product sciences 质谱法是天然产物科学的统一力量。

IF 12.5 1区化学

Natural Product Reports Pub Date : 2026-06-15 DOI: 10.1039/D6NP90018F

Mehdi A. Beniddir, Nadja B. Cech, Roger Linington and Neha Garg

引用次数: 0

Emerging technologies for the discovery of biosynthetic genes in plants. 发现植物生物合成基因的新兴技术。