'Need for speed: high throughput' - mass spectrometry approaches for high-throughput directed evolution screening of natural product enzymes.

IF 10.2 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Natural Product Reports Pub Date : 2025-02-27 DOI:10.1039/d4np00053f

Robert A Shepherd, Conrad A Fihn, Alex J Tabag, Shaun M K McKinnie, Laura M Sanchez

引用次数: 0

Abstract

Covering: 2015 to 2024Mass spectrometry (MS)-based methods have been implemented extensively for enzyme engineering due to their label-free nature, making them suitable for screening a wide range of biochemical systems. Over the past decade, advancements in mass spectrometry, separation science, and the implementation of hyphenated methods have allowed for more streamlined analysis of large volumes of samples while maximizing the richness and dimensionality of the data collected. In this review we highlight recent advancements in mass spectrometry that have allowed for more efficient, robust, and rigorous enzyme engineering for various applications relating to natural products chemistry.

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“对速度的需求：高通量”-天然产物酶的高通量定向进化筛选的质谱方法。

基于质谱（MS）的方法由于其无标记的性质，已广泛应用于酶工程，使其适用于筛选广泛的生化系统。在过去的十年中，质谱、分离科学和连字符方法的进步使得对大量样品进行更精简的分析成为可能，同时最大限度地提高了所收集数据的丰富性和维度。在这篇综述中，我们重点介绍了质谱技术的最新进展，这些进展使酶工程在与天然产物化学有关的各种应用中更加高效、稳健和严格。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Natural Product Reports 化学-生化与分子生物学

CiteScore

21.20

自引率

3.40%

发文量

127

审稿时长

1.7 months

期刊介绍： Natural Product Reports (NPR) serves as a pivotal critical review journal propelling advancements in all facets of natural products research, encompassing isolation, structural and stereochemical determination, biosynthesis, biological activity, and synthesis. With a broad scope, NPR extends its influence into the wider bioinorganic, bioorganic, and chemical biology communities. Covering areas such as enzymology, nucleic acids, genetics, chemical ecology, carbohydrates, primary and secondary metabolism, and analytical techniques, the journal provides insightful articles focusing on key developments shaping the field, rather than offering exhaustive overviews of all results. NPR encourages authors to infuse their perspectives on developments, trends, and future directions, fostering a dynamic exchange of ideas within the natural products research community.