Cardiology Research and Practice最新文献

{"title":"Outcomes of Combined Heart-Kidney Transplantation in Older Recipients.","authors":"Curry Sherard,&nbsp;Vineeth Sama,&nbsp;Jennie H Kwon,&nbsp;Khaled Shorbaji,&nbsp;Lauren V Huckaby,&nbsp;Brett A Welch,&nbsp;Chakradhari Inampudi,&nbsp;Ryan J Tedford,&nbsp;Arman Kilic","doi":"10.1155/2023/4528828","DOIUrl":"https://doi.org/10.1155/2023/4528828","url":null,"abstract":"<p><strong>Objectives: </strong>The upper limit of recipient age for combined heart-kidney transplantation (HKT) remains controversial. This study evaluated the outcomes of HKT in patients aged ≥65 years.</p><p><strong>Methods: </strong>The United Network of Organ Sharing (UNOS) was used to identify patients undergoing HKT from 2005 to 2021. Patients were stratified by age at transplantation: <65 and ≥ 65 years. The primary outcome was one-year mortality. Secondary outcomes included 90-day and 5-year mortality, postoperative new-onset dialysis, postoperative stroke, acute rejection prior to discharge, and rejection within one-year of HKT. Survival was compared using Kaplan-Meier analysis, and risk adjustment for mortality was performed using Cox proportional hazards modeling.</p><p><strong>Results: </strong>HKT in recipients aged ≥65 significantly increased from 5.6% of all recipients in 2005 to 23.7% in 2021 (<i>p</i>=0.002). Of 2,022 HKT patients in the study period, 372 (18.40%) were aged ≥65. Older recipients were more likely to be male and white, and fewer required dialysis prior to HKT. There were no differences between cohorts in unadjusted 90-day, 1-year, or 5-year survival in Kaplan-Meier analysis. These findings persisted after risk-adjustment, with an adjusted hazard for one-year mortality for age ≥65 of 0.91 (95% CI (0.63-1.29), <i>p</i>=0.572). As a continuous variable, increasing age was not associated with one-year mortality (HR 1.01 (95% CI (1.00-1.02), <i>p</i>=0.236) per year). Patients aged ≥65 more frequently required new-onset dialysis prior to discharge (11.56% vs. 7.82%, <i>p</i>=0.051). Stroke and rejection rates were comparable.</p><p><strong>Conclusion: </strong>Combined HKT is increasing in older recipients, and advanced age ≥65 should not preclude HKT.</p>","PeriodicalId":9494,"journal":{"name":"Cardiology Research and Practice","volume":"2023 ","pages":"4528828"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10314816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10121291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant Adenovirus siRNA Knocking Down the Ndufs4 Gene Alleviates Myocardial Apoptosis Induced by Oxidative Stress Injury.","authors":"Beibei Wang,&nbsp;Jinsheng Zhang,&nbsp;Aijun Xu","doi":"10.1155/2023/8141129","DOIUrl":"https://doi.org/10.1155/2023/8141129","url":null,"abstract":"<p><p>Oxidative stress results in myocardial cell apoptosis and even life-threatening heart failure in myocardial ischemia-reperfusion injury. Specific blocking of the complex I could reduce cell apoptosis. Ndufs4 is a nuclear-encoded subunit of the mitochondrial complex I and participates in the electron transport chain. In this study, we designed and synthesized siRNA sequences knocking down the rat Ndufs4 gene, constructed recombinant adenovirus Ndufs4 siRNA (Ad-Ndufs4 siRNA), and primarily verified the role of Ndufs4 in oxidative stress injury. The results showed that the adenovirus infection rate was about 90%, and Ndufs4 mRNA and protein were decreased by 76.7% and 64.9%, respectively. Furthermore, the flow cytometry assay indicated that the cell apoptosis rate of the Ndufs4 siRNA group was significantly decreased as compared with the H₂O₂-treated group. In conclusion, we successfully constructed Ndufs4 siRNA recombinant adenovirus; furthermore, the downexpression of the Ndufs4 gene may alleviate H₂O₂-induced H9c2 cell apoptosis.</p>","PeriodicalId":9494,"journal":{"name":"Cardiology Research and Practice","volume":"2023 ","pages":"8141129"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9897913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9229248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: The Long-Term Change of Arrhythmias after Transcatheter Closure of Perimembranous Ventricular Septal Defects.","authors":"Cardiology Research And Practice","doi":"10.1155/2023/9898161","DOIUrl":"https://doi.org/10.1155/2023/9898161","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2021/1625915.].</p>","PeriodicalId":9494,"journal":{"name":"Cardiology Research and Practice","volume":"2023 ","pages":"9898161"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10447158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10061871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SFRP4 Reduces Atherosclerosis Plaque Formation in ApoE Deficient Mice.","authors":"Hua Guan,&nbsp;Ting Liu,&nbsp;Miaomiao Liu,&nbsp;Xue Wang,&nbsp;Tao Shi,&nbsp;Fengwei Guo","doi":"10.1155/2023/8302289","DOIUrl":"https://doi.org/10.1155/2023/8302289","url":null,"abstract":"<p><p>Secreted frizzled related protein 4 (SFRP4), a member of the SFRPs family, contributes to a significant function in metabolic and cardiovascular diseases. However, there is not enough evidence to prove the antiatherosclerosis effect of SFRP4 in ApoE knock-out (KO) mice. ApoE KO mice were fed a western diet and injected adenovirus (Ad)-SFRP4 through the tail vein for 12 weeks. Contrasted with the control cohort, the area of atherosclerotic plaque in ApoE KO mice overexpressing SFRP4 was reduced significantly. Plasma high-density lipoprotein cholesterol was elevated in the Ad-SFRP4 group. RNA sequence analysis indicated that there were 96 differentially expressed genes enriched in 10 signaling pathways in the mRNA profile of aortic atherosclerosis lesions. The analysis data also revealed the expression of a number of genes linked to metabolism, organism system, and human disease. In summary, our data demonstrates that SFRP4 could play an important role in improving atherosclerotic plaque formation in the aorta.</p>","PeriodicalId":9494,"journal":{"name":"Cardiology Research and Practice","volume":"2023 ","pages":"8302289"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10261310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valve-Sparing Aortic Root Replacement Technique: Valsalva Graft versus Two Straight Tubular Grafts.","authors":"Alexander Makkinejad,&nbsp;Bailey Brown,&nbsp;Rana-Armaghan Ahmad,&nbsp;Joanna Hua,&nbsp;Xiaoting Wu,&nbsp;Shinichi Fukuhara,&nbsp;Karen Kim,&nbsp;Himanshu Patel,&nbsp;G Michael Deeb,&nbsp;Bo Yang","doi":"10.1155/2023/4076881","DOIUrl":"https://doi.org/10.1155/2023/4076881","url":null,"abstract":"<p><strong>Background: </strong>There are many variations in valve-sparing aortic root replacement techniques. Our aim is to determine the impact of the graft on mid-term outcomes: Valsalva graft vs. two straight tubular grafts.</p><p><strong>Methods: </strong>From 2004 to 2020, 332 patients underwent valve-sparing aortic root replacement with either a Valsalva graft (Valsalva group: <i>n</i> = 270) or two straight tubular grafts (two-graft group: <i>n</i> = 62). Data were obtained through chart review and the National Death Index. Primary outcomes were mid-term survival and freedom from reoperation.</p><p><strong>Results: </strong>The preoperative characteristics of the groups were similar, but the two-graft group had more type A dissections (32% vs. 19%) and emergent operations (26% vs. 15%) and was younger (45 vs. 50 years). Intraoperatively, the groups were similar, but the two-graft group had longer cross-clamp (245 vs. 215 minutes) and cardiopulmonary bypass times (284 vs. 255 minutes). Postoperative complications including reoperation for bleeding, stroke, pacemaker implantation, and renal failure were slightly more frequent in the Valsalva group, but the differences were not significant. Operative mortality was similar between the Valsalva and two-graft groups (0.7% vs. 0%). Five-year survival in the two-graft group was 100% compared to 96% in the Valsalva group (<i>p</i>=0.56). Five-year freedom from reoperation in the two-graft group was 100% compared to 93% in the Valsalva group (<i>p</i>=0.29).</p><p><strong>Conclusions: </strong>The Valsalva and two-graft techniques both have excellent short- and mid-term outcomes. The two-graft technique might have slightly better survival and freedom from reoperation, but a larger sample size and longer follow-up are needed to determine if these advantages are significant.</p>","PeriodicalId":9494,"journal":{"name":"Cardiology Research and Practice","volume":"2023 ","pages":"4076881"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9918367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10707873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Small Increase in Serum Creatinine within 48 h of Hospital Admission Is an Independent Predictor of In-Hospital Adverse Outcomes in Patients with ST-Segment Elevation Myocardial Infarction Undergoing Primary Percutaneous Coronary Intervention: Findings from the Improving Care for Cardiovascular Disease in the China Project.","authors":"Jiajia Zhu,&nbsp;Wenxian Liu,&nbsp;Jiang Li,&nbsp;Changsheng Ma,&nbsp;Dong Zhao","doi":"10.1155/2023/1374206","DOIUrl":"https://doi.org/10.1155/2023/1374206","url":null,"abstract":"<p><strong>Background: </strong>Acute kidney injury (AKI) is a common complication of percutaneous coronary intervention (PCI) that has been associated with high morbidity and mortality in patients with STEMI. Acute tubular damage may be reflected by serum creatinine (Scr) values that do not meet the criteria for AKI.</p><p><strong>Methods: </strong>This analysis included 19,424 patients from the Improving Care for Cardiovascular Disease in China, Acute Coronary Syndrome Project (<i>n</i> = 5,221 (36.8%), patients with a small increase in Scr within 48 h of hospitalization; <i>n</i> = 14,203 patients with no increase in Scr). The primary outcome was the incidence of major adverse cardiovascular events (MACE). Secondary outcomes included the incidence of massive hemorrhage, in-hospital death, atrial fibrillation, heart failure, cardiogenic shock, cardiac arrest, and stroke. Logistic regression analysis was used to evaluate associations between a small increase in Scr within 48 h of hospitalization (>0.1 to <0.3 mg/dl) and MACE or massive hemorrhage during hospitalization.</p><p><strong>Results: </strong>Patients with a small increase in Scr within 48 h of hospitalization were significantly more likely to experience MACE (11.2% vs. 9.1%; <i>P</i> < 0.001) or massive hemorrhage (3.2% vs. 2.2%; <i>P</i> < 0.001) compared to patients with no increase in Scr, but there was no significant difference in in-hospital mortality (0.8% vs. 0.9%; <i>P</i>=0.301). Logistic regression analysis showed that a small increase in Scr within 48 h of hospital admission was a risk factor for MACE (OR, 1.168; 95% CI, 1.044-1.306; <i>P</i>=0.006) or massive hemorrhage (OR, 1.413; 95% CI, 1.164-1.715; <i>P</i> < 0.001). Other risk factors included age ˃65 years, history of heart failure, use of glycoprotein IIb/IIIa inhibitors, aspirin or ACEI/ARB, LVEF <40%, Killip class III-IV, and increased SBP and heart rate.</p><p><strong>Conclusion: </strong>A small increase in Scr during hospitalization in patients with STEMI undergoing primary PCI that does not meet the criteria for AKI is a risk factor for in-hospital adverse outcomes. This effect is maintained in patients with normal Scr at hospitalization. <i>Trial Registration</i>. Clinical trial registration: URL: https://www.clinicaltrials.gov. Unique identifier: NCT02306616.</p>","PeriodicalId":9494,"journal":{"name":"Cardiology Research and Practice","volume":"2023 ","pages":"1374206"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9272007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early and Late Mortality Predictors in Patients with Acute Aortic Dissection Type B.","authors":"Ratko M Lasica,&nbsp;Jovan P Perunicic,&nbsp;Dejana R Popovic,&nbsp;Igor B Mrdovic,&nbsp;Ross A Arena,&nbsp;Nebojsa L Radovanovic,&nbsp;Mina R Radosavljevic-Radovanovic,&nbsp;Lazar D Djukanovic,&nbsp;Milika R Asanin","doi":"10.1155/2022/7869356","DOIUrl":"https://doi.org/10.1155/2022/7869356","url":null,"abstract":"<p><strong>Background/aim: </strong>Despite technological advances in diagnosis and treatment, in-hospital mortality with acute aortic dissection type B is still about 11%. The purpose of this study was to assess the risk factors for early and long-term adverse outcomes in patients with acute aortic dissection type B treated medically or with conventional open surgery.</p><p><strong>Methods: </strong>The present study included 104 consecutive patients with acute aortic dissection type B treated in our Center from January 1^st, 1998 to January 1^st, 2007. Patient demographic and clinical characteristics as well as in-hospital complications were reviewed. Univariate and multivariate testing was performed to identify the predictors of in-hospital (30-day) and late (within 9 years) mortality.</p><p><strong>Results: </strong>92 (88.5%) patients were treated medically, while 12 (11.5%) patients with complicated acute aortic dissection type B were treated by open surgical repair. In-hospital complications occurred in 35.7% patients, the most often being acute renal failure (28%), hypotension/shock (24%), mesenteric ischemia (12%), and limb ischemia (8%). The in-hospital mortality rate was 15.7% and the 9-year mortality rate was 51.9%. Independent predictors of early mortality in patients with acute aortic dissection type B were uncontrolled hypertension (HR-20.69) and a dissecting aorta diameter >4.75 cm (HR-6.30). Independent predictors of late mortality were relapsing pain (HR-7.93), uncontrolled hypertension (HR-7.25), and a pathologic difference in arterial blood pressure (>20 mmHg) (HR-5.33).</p><p><strong>Conclusion: </strong>Knowledge of key risk factors may help with a better choice of treatment and mortality reduction in acute aortic dissection type B patients.</p>","PeriodicalId":9494,"journal":{"name":"Cardiology Research and Practice","volume":" ","pages":"7869356"},"PeriodicalIF":2.1,"publicationDate":"2022-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9719417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35349201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Novel Blunt Dissection Technique for Surgical Unroofing in Myocardial Bridging Patients.","authors":"Xiangyang Wu,&nbsp;Jie Zhu,&nbsp;Yong Mao,&nbsp;Xinqiang Guan,&nbsp;Xiaofeng He,&nbsp;Qi Ma,&nbsp;Xiaopeng Zhang,&nbsp;Shixiong Wang,&nbsp;Yongnan Li","doi":"10.1155/2022/2370802","DOIUrl":"https://doi.org/10.1155/2022/2370802","url":null,"abstract":"<p><strong>Background: </strong>Myocardial bridging (MB) is a congenital anomaly involving the myocardial tissue encasement of a segment of the coronary artery. The purpose of the present study was to assess safety and efficacy of two surgical methods used for treating MB patients at our institute.</p><p><strong>Methods: </strong>Off-pump MB unroofing was performed in 45 adult patients between January 2016 and December 2021 by traditional surgical unroofing techniques (conventional group, <i>n</i> = 26) and blunt dissection techniques (blunt dissection group, <i>n</i> = 19). We retrospectively reviewed our patients by examining the baseline clinical characteristics, risk factors, medications, and diagnostic data for coronary artery disease. The Seattle Angina Questionnaire (SAQ) was used to assess angina symptoms both preoperatively and 6 months postsurgery.</p><p><strong>Results: </strong>No significant difference in preoperative clinical characteristics was observed between the two groups. The blunt dissection group had shorter unroofed period (14.69 vs. 18.91 mins, <i>P</i>=0.001), less ventilator time (16.26 vs. 24.62 hours, <i>P</i> < 0.001), and a shorter hospital stay (8.74 vs. 12.89 days, <i>P</i> < 0.001). Although both traditional and blunt dissection techniques significantly improved postoperative SAQ scores including physical limitation due to angina, anginal stability, anginal frequency, treatment satisfaction, and quality of life (<i>P</i> < 0.001), no significant difference was observed between the traditional and blunt dissection techniques for SAQ. No cases of left anterior descending (LAD) injury in the blunt dissection group were observed although seven patients in the conventional group had LAD injuries.</p><p><strong>Conclusions: </strong>In our single-center experience of MB unroofing, the blunt dissection technique is a safe, effective technique that significantly reduces surgical and ventilator time and hospital stay. MB patients with severe angina who underwent the blunt dissection for surgical unroofing experienced significant improvements in anginal symptoms and quality of life six months after the surgery.</p>","PeriodicalId":9494,"journal":{"name":"Cardiology Research and Practice","volume":" ","pages":"2370802"},"PeriodicalIF":2.1,"publicationDate":"2022-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40504181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Systemic Immune-Inflammation Index May Predict the Coronary Slow Flow Better Than High-Sensitivity C-Reactive Protein in Patients Undergoing Elective Coronary Angiography.","authors":"Kurtulus Karauzum,&nbsp;Irem Karauzum,&nbsp;Kaan Hanci,&nbsp;Dogus Gokcek,&nbsp;Beyzanur Gunay,&nbsp;Hussain Bakhshian,&nbsp;Tayfun Sahin,&nbsp;Ertan Ural","doi":"10.1155/2022/7344639","DOIUrl":"https://doi.org/10.1155/2022/7344639","url":null,"abstract":"<p><strong>Methods: </strong>A total of 197 patients (102 patients with CSF; 95 patients with normal coronary flow) were included in this retrospective study. Clinical and angiographic characteristics of patients were obtained from hospital records.</p><p><strong>Results: </strong>Patients with CSF had higher SII, neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte (PLR), and high-sensitivity C-reactive protein (hsCRP) levels compared with the control group. Body mass index (<i>p</i>=0.022, OR 1.151, 95% CI 1.121-1.299), low-density lipoprotein (<i>p</i>=0.018, OR 1.028, 95% CI 1.005-1.052), hsCRP (<i>p</i>=0.044, OR 1.161, 95% CI 1.004-1.343), and SII (<i>p</i> < 0.001, OR 1.015, 95% CI 1.003-1.026) were independent predictors of CSF in the multivariable analysis. The optimal cutoff value of SII in predicting CSF was >877 in ROC curve analysis (<i>p</i> < 0.001, AUC = 0.892, 95% CI 0.848-0.936). This cutoff value of SII predicted the CSF with a sensitivity of 71.5% and specificity of 92.4%. Spearman correlation analysis showed a positive correlation between the mean TFC value and PLR, NLR, hsCRP, and SII.</p><p><strong>Conclusions: </strong>SII may be used as a better indicator for the prediction of CSF than hsCRP.</p>","PeriodicalId":9494,"journal":{"name":"Cardiology Research and Practice","volume":" ","pages":"7344639"},"PeriodicalIF":2.1,"publicationDate":"2022-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40504179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atorvastatin Inhibits Ferroptosis of H9C2 Cells by regulatingSMAD7/Hepcidin Expression to Improve Ischemia-Reperfusion Injury.","authors":"You Peng,&nbsp;Bin Liao,&nbsp;Yan Zhou,&nbsp;Wei Zeng,&nbsp;Zhi-Yu Zeng","doi":"10.1155/2022/3972829","DOIUrl":"https://doi.org/10.1155/2022/3972829","url":null,"abstract":"<p><strong>Background: </strong>Ferroptosis plays a key role in cardiomyopathy. Atorvastatin (ATV) has a protective effect on ischemia-reperfusion (I/R) cardiomyopathy. The purpose of this study is to elucidate the mechanism of ATV in I/R injury.</p><p><strong>Methods: </strong>H9C2 cells and cardiomyopathy rats were induced by hypoxia/reoxygenation (H/R) and I/R to construct <i>in vitro</i> and <i>in vivo</i> models. Cell viability was determined by CCK8. Cardiac histopathology was observed by HE staining. Transmission electron microscope (TEM) was used to observe the mitochondrial morphology. The reactive oxygen species (ROS) content in cells was analyzed by the biochemical method. ELISA was conducted to calculate the concentrations of total iron/Fe²⁺ and hepcidin. The expression of ferroptosis and SMAD pathway-related genes were detected by qPCR. Western blot was performed to detect the expression levels of ferroptosis and SMAD pathway-related proteins.</p><p><strong>Results: </strong>In H9C2 cells, ATV reversed the decline in cell viability, mitochondrial shrinkage, and ROS elevation induced by erastin or H/R. The concentration of total iron and Fe²⁺ in H/R-induced H9C2 cells increased, and the protein expression of FPN1 decreased. After ATV treatment, the concentration of total iron and Fe²⁺ decreased, and the protein expression of FPN1 increased. The expression of the SMAD7 gene in H/R-induced H9C2 cells decreased, and the expression of the hepcidin gene increased, which were reversed by ATV. When SMAD7 was knocked down, ATV treatment failed to produce the above effect. ATV also improved ferroptosis in I/R rat myocardium through the SMAD7/hepcidin pathway.</p><p><strong>Conclusions: </strong>ATV reversed the decline in H9C2 cell viability, mitochondrial shrinkage, and ROS elevation, and improved the myocardium ferroptosis through the SMAD7/hepcidin pathway in I/R rat.</p>","PeriodicalId":9494,"journal":{"name":"Cardiology Research and Practice","volume":" ","pages":"3972829"},"PeriodicalIF":2.1,"publicationDate":"2022-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9666047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40498280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}