{"title":"The carbohydrate components of ovine luteinizing hormone","authors":"Earl F. Walborg Jr., Darrell N. Ward","doi":"10.1016/0006-3002(63)91641-X","DOIUrl":"10.1016/0006-3002(63)91641-X","url":null,"abstract":"<div><p>The quantitative analysis of the carbohydrate components of ovine luteinizing hormone demonstrated the presence of 8 residues of <em>N</em>-acetylglucosamine, 3 residues of <em>N</em>-acetylgalactosamine, 7 residues of mannose, 2 residues of galactose, 1 residue of fucose and 0–1 residue of sialic acid per molecule. The number of residues of each carbohydrate subunit was calculated assuming a molecular weight of 28 000.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 2","pages":"Pages 304-312"},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91641-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23683019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The resistance to dispersion of collagen fibres formed in vitro in the presence of ascorbic acid","authors":"J.K. Candlish, G.R. Tristram","doi":"10.1016/0006-3002(63)91639-1","DOIUrl":"10.1016/0006-3002(63)91639-1","url":null,"abstract":"<div><p>Collagen fibres have been allowed to form by warming soluble collagen at pH 7.4 to 37° in the presence of amounts of ascorbic acid which are within the physiological range (up to 10<sup>−4</sup> M). Such fibres are more resistant to dispersion by denaturants, enzymes with little or no group specificity, and ultrasonic treatment than fibres formed in the presence of buffer alone. This stabilising effect appears to be associated with the enol grouping of ascorbic acid. The possibility of an analogous stabilisation mechanism <em>in vivo</em> is discussed.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 2","pages":"Pages 289-294"},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91639-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23683017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Metabolism of chondroitinsulphate in the epiphyseal plate of puppies","authors":"J.M. Bowness","doi":"10.1016/0006-3002(63)91640-8","DOIUrl":"10.1016/0006-3002(63)91640-8","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The infrared spectra of partially purified acid mucopolysaccharide preparations from epiphyseal plate showed bands characteristic of chondroitinsulphate C as well as A, though no sharp chromatographic separation of the two was obtained.</p></span></li><li><span>2.</span><span><p>2. Chromatography of unfractionated epiphyseal-plate extracts showed the presence of two protein peaks whose position in the elution pattern roughly coincided with two chondroitinsulphate peaks.</p></span></li><li><span>3.</span><span><p>3. The rates of metabolism of the mixed chondroitinsulphates of various tissue extracts have been compared by the use of radioactive sulphate. The epiphyseal-plate chondroitinsulphate was metabolished at a much faster rate than from ribs. The chondroitinsulphate extractable only with alkali was distinguished from that extractable with water by a lower rate of metabolism, particularly evident in the extract of the epiphyseal-metaphyseal junction, and by the infrared spectrum, which indicates that it has a lower content of chondroitinsulphate C.</p></span></li><li><span>4.</span><span><p>4. Chromatography of aqueous epiphyseal-plate extracts obtained at various times after injection of Na<sub>2</sub><sup>35</sup>SO<sub>4</sub> demonstrated the depolymerisation <em>in vivo</em> of chondroitinsulphate, mainly prior to desulphation.</p></span></li></ul></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 2","pages":"Pages 295-303"},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91640-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23683018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The distribution of acid mucopolysaccharides in skin of diabetic rats","authors":"Sara Schiller, Albert Dorfman","doi":"10.1016/0006-3002(63)91649-4","DOIUrl":"10.1016/0006-3002(63)91649-4","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 2","pages":"Pages 371-373"},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91649-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23682783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The autolysis of cell walls of Streptococcus faecalis","authors":"M.D. Montague","doi":"10.1016/0006-3002(63)91650-0","DOIUrl":"10.1016/0006-3002(63)91650-0","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 2","pages":"Pages 373-376"},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91650-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23682784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative biochemistry of skin the mucopolysaccharides of shark skin","authors":"Nobuko Seno, Karl Meyer","doi":"10.1016/0006-3002(63)91636-6","DOIUrl":"10.1016/0006-3002(63)91636-6","url":null,"abstract":"<div><p>Mucopolysaccharides were obtained and fractionated from the skin of two eleasmobranch fishes, the Blue shark and Sandbar shark. In both species, the main polysaccharide fractions were hyaluronic acid, and chondroitin sulfate B. The total mucopolysaccharide content, however, varied considerably. In both species, the chondroitin sulfate B fractions were oversulfated. In both species, keratosulfate appeared to be present in small amounts.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 2","pages":"Pages 258-264"},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91636-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23683014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"On the interaction between polysaccharides and other macromolecules","authors":"Torvard C. Laurent , Ingemar Björk , Adolph Pietruszkiewicz , Håkan Persson","doi":"10.1016/0006-3002(63)91645-7","DOIUrl":"10.1016/0006-3002(63)91645-7","url":null,"abstract":"<div><p>A study of the diffusion behaviour of albumin, α-crystallin, fribrinogen, and turnip yellow mosaic virus in hyaluronic acid media has shown that the diffusion rates of these substances are markedly decreased by the presence of the polysaccharide. The relative decrease in the same as the corresponding effect observed on the sedimentation rates of these substances.</p><p>Investigation of eleven globular particles with diameters of 47–3650 Å established that the relative decrease of the sedimentation rate of a particle in the presence of hyaluronic acid was essentially a function of the diameter of the particle and the concentration of the polysaccharide and that the effect could be expressed by a simple exponential relationship. The results observed have been interpreted as a macromolecular-sieving effect of the polysaccharide.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 2","pages":"Pages 351-359"},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91645-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23682779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preparation of a glycopeptide from ovine interstitial cell-stimulating hormone","authors":"Harold Papkoff","doi":"10.1016/0006-3002(63)91654-8","DOIUrl":"10.1016/0006-3002(63)91654-8","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 2","pages":"Pages 384-387"},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91654-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23682787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}