{"title":"Studies on the glucose-transport system in Escherichia coli with α-methylglucoside as substrate","authors":"H. Hagihira, T.H. Wilson, E.C.C. Lin","doi":"10.1016/0006-3002(63)90912-0","DOIUrl":"10.1016/0006-3002(63)90912-0","url":null,"abstract":"<div><p>The properties of the glucose-transport system of <em>Escherichia coli</em> K10 were studied by following intracellular accumulation of <sup>14</sup>C-labeled α-methylglucoside. A mutant defective in the uptake of glucose was found to be unable to accumulate α-methylglucoside to a significant level, confirming the view that the carrier for glucose also acts on α-methylglucoside. Although the glucoside was not incorporated into cellular constituents or metabolized for energy, about one-half of the accumulated molecules underwent phosphorylation while one-half remained as the free glucoside. Studies on the inhibition of uptake of the glucoside showed that the affinity of the compound depended upon the substituents on C-2, C-3 and C-6. Structural modifications at C-1, on the other hand, had relatively little effect on the affinity unless a large aglycone was added. α-Methylglucoside accumulated by the cells was gradually lost upon dilution of the cells in simple inorganic medium. The rate of exit could be accelerated by two groups of compounds: non-metabolizable structural analogs with affinity for the transport carrier and metabolizable compounds which entered the cell by other routes. The effect of the latter group depended upon their further metabolism within the cell rather than their own direct effects on the carrier system.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 505-515"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90912-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23670749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ubiquinone-50 and plastoquinone-45 in plant tissue cultures of Paul's scarlet rose","authors":"D.R. Threlfall, T.W. Goodwin","doi":"10.1016/0006-3002(63)90916-8","DOIUrl":"10.1016/0006-3002(63)90916-8","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 532-534"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90916-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Use of antibody bound to modified cellulose as an immunospecific adsorbent of antigens","authors":"A.T. Jagendorf , A. Patchornik , M. Sela","doi":"10.1016/0006-3002(63)90913-2","DOIUrl":"10.1016/0006-3002(63)90913-2","url":null,"abstract":"<div><p>Rabbit serum globulins were complexed with bromoacetyl cellulose. Under appropriate conditions 96 to 98% of the protein was chemically bound. Binding of the globulins required a preliminary physical adsorption followed by a chemical reaction. The solid globulin-cellulose complex, under the conditions used, adsorbed specific antigens from solution completely, up to a maximum amount which represented a ratio of antibody to antigen close to that found in a specific precipitate in the equivalence zone. Preliminary experiments are reported on the problems of the specificity of adsorption of homologous antigen compared to unrelated proteins, and of the elution of adsorbed antigen. Potential uses for this method in biochemical investigations are suggested.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 516-527"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90913-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23670750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Caractérisation de la l-thréo-β-hydroxyleucine à partir de Deutzia gracilis","authors":"J. Jadot, J. Casimir, F. Alderweireldt","doi":"10.1016/0006-3002(63)90911-9","DOIUrl":"10.1016/0006-3002(63)90911-9","url":null,"abstract":"<div><p><span>l</span>-<em>threo</em>-β-Hydroxyleucine has been isolated from <em>Deutzia gracilis</em> by ion-exchange resin chromatography, and by preparative paper chromatography. The substance is reduced to leucine by hydroiodic acid and offers the characteristic reaction of β-hydroxyamino acids with periodic acid. Alkaline decomposition confirms the β position of the alcohol function. The natural substance and the <span>dl</span>-<em>threo</em>-β-hydroxyleucine are inseparable by paper chromatography, and the study of their nuclear-magnetic-resonance spectra brings supplementary arguments in favour of the <em>threo</em> form. The natural substance has the same infrared spectrum as synthetic <em>threo</em>-β-hydroxyleucine.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 500-504"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90911-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80817816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ralph G. Wieland, Richard P. Levy, David Katz, H. Hirschmann
{"title":"Evidence for secretion of 3β-hydroxyandrost-5-en-17-one sulfate by measurement in normal human adrenal venous blood","authors":"Ralph G. Wieland, Richard P. Levy, David Katz, H. Hirschmann","doi":"10.1016/0006-3002(63)90930-2","DOIUrl":"10.1016/0006-3002(63)90930-2","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 566-568"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90930-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Metabolism in vivo of steroids by the canine testes","authors":"Kristen B. Eik-nes, Madhukar Kekre","doi":"10.1016/0006-3002(63)90906-5","DOIUrl":"10.1016/0006-3002(63)90906-5","url":null,"abstract":"<div><p>The formation of testosterone <em>in vivo</em> has been investigated in anesthetized dogs by infusing radioactive steroids <em>via</em> the left spermatic artery and isolating radioactive testosterone in venous blood obtained from the left spermatic vein. The data from such experiments indicate that the formation of 17α-hydroxypregnenolone and the metabolism of this steroid to testosterone is a major route in the biosynthesis <em>in vivo</em> of testosterone from pregnenolone by the canine testis. </p><ul><li><span>1.</span><span><p>1. When an equal amount of [4<sup>−14</sup>C]progesterone and 17α-hydroxy[7α-<sup>3</sup>H]pregnenolone were infused at a constant rate by the spermatic artery, the concentration of <sup>3</sup>H in isolated spermatic vein testosterone was from 3–8 times that of <sup>14</sup>C.</p></span></li><li><span>2.</span><span><p>2. When [7α-<sup>3</sup>H]prenenolone was infused by the left spermatic artery for 5 min, more [<sup>3</sup>H]17α-hydroxypregnenolone than [<sup>3</sup>H]progesterone was found in the left testis at the end of this infusion.</p></span></li><li><span>3.</span><span><p>3. The problem of preferred metabolism to testosterone <em>in vivo</em> by testicular tissue is discussed.</p></span></li></ul></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 449-456"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90906-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23670744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chicken antibodies to bovine serum albumin molecular size and sensitivity to 2-mercaptoethanol","authors":"Grace L. Rosenquist , R.V. Gilden","doi":"10.1016/0006-3002(63)90920-X","DOIUrl":"10.1016/0006-3002(63)90920-X","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 543-545"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90920-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Conversion of retro-vitamin A to vitamin A in the vitamin A-deficient rat","authors":"T.N.R. Varma , T.K. Murray","doi":"10.1016/0006-3002(63)90925-9","DOIUrl":"10.1016/0006-3002(63)90925-9","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 556-558"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90925-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Incorporation of oxygen atoms from atmospheric oxygen into indigotin by Pseudomonas indoloxidans","authors":"Tairo Oshima , Sadaaki Kawai , Fujio Egami","doi":"10.1016/0006-3002(63)90926-0","DOIUrl":"10.1016/0006-3002(63)90926-0","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 558-559"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90926-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Disulphide bridges and a suggested structure of chymotrypsinogen","authors":"B. Keil, Z. Prusík, F. Šorm","doi":"10.1016/0006-3002(63)90927-2","DOIUrl":"10.1016/0006-3002(63)90927-2","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 559-561"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90927-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}