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Biochimica et biophysica acta最新文献

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Studies on glycoproteins X. Equilibrium measurements on influenza virus-glycoproteins systems 糖蛋白的研究。流感病毒-糖蛋白系统的平衡测量
Biochimica et biophysica acta Pub Date : 1963-10-29 DOI: 10.1016/0006-3002(63)91635-4
S.Fazekas De St. Groth, A. Gottschalk
{"title":"Studies on glycoproteins X. Equilibrium measurements on influenza virus-glycoproteins systems","authors":"S.Fazekas De St. Groth,&nbsp;A. Gottschalk","doi":"10.1016/0006-3002(63)91635-4","DOIUrl":"https://doi.org/10.1016/0006-3002(63)91635-4","url":null,"abstract":"<div><p>Measurements in systems of enzymically inactive influenza viruses and sialoglycoproteins disclosed an equilibrium between the free reactants and the virus-glycoprotein complex. Equilibrium constants were determined by two methods: equilibrium filtration and ultracentrifugal separation. The glycoproteins tested were influenza virus haemagglutinin inhibitors of high potency (ovine submaxially glycoprotein) and low potency (fetuin, orosomucoid and trypsin-treated OSM). The equilibrium constants (in molar terms) were found to be 1·10<sup>−8</sup> for OSM, 9·10<sup>−7</sup> for fetuin, 2·10<sup>−5</sup> for orosomucoid and 4·10<sup>−6</sup> for the glycopeptides (assumed mol. wt. 50000) obtained by controlled trypsin treatment of OSM. Weight for weight OSM was 17 times more firmly bound to enzymically inactive Lee virus than were its constituent glycopeptides as formed on trypsin treatment.</p><p>The number of negatively charged terminal sialic acid residues attached simultaneously to complementary groupings at the virus surface, appears to determine primarily the inhibitory potency of the glycoproteins. This determinant is a compound of the number of sialic acid residues available per glycoprotein molecule and of the complementariness of the contacting areas on the surface of virus and glycoprotein.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91635-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91755305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 21
On the interaction between polysaccharides and other macromolecules 多糖与其他大分子的相互作用
Biochimica et biophysica acta Pub Date : 1963-10-29 DOI: 10.1016/0006-3002(63)91646-9
Torvard C. Laurent, Håkan Persson
{"title":"On the interaction between polysaccharides and other macromolecules","authors":"Torvard C. Laurent,&nbsp;Håkan Persson","doi":"10.1016/0006-3002(63)91646-9","DOIUrl":"10.1016/0006-3002(63)91646-9","url":null,"abstract":"<div><p>Advantage has been taken of the macromoleculer-sieve effect of hyaluronic acid to increase resolution in the ultracentrifuge. Substances, which normally sediment at the same rate in salt solutions because of a similarity in their sedimentation coefficients can be separated in hyaluronic acid media if they differ in size and shape. By selecting suitable concentrations of hyaluronic acid it has proved possible to separate fibrinogen from γ-globulin; albumin from dextran; and a colloidal silica from a polystyrene latex. Like procedures revealed and unsuspected inhomogeneity in a dextran preparation and served to resolve the polyglucose into two fractions.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91646-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23682780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Structure du mycoside C2 de Mycobacterium avium 鸟分枝杆菌真菌蛋白C2结构
Biochimica et biophysica acta Pub Date : 1963-10-29 DOI: 10.1016/0006-3002(63)91643-3
M Chaput , G Michel , E Lederer
{"title":"Structure du mycoside C2 de Mycobacterium avium","authors":"M Chaput ,&nbsp;G Michel ,&nbsp;E Lederer","doi":"10.1016/0006-3002(63)91643-3","DOIUrl":"10.1016/0006-3002(63)91643-3","url":null,"abstract":"<div><p>Mycoside C<sub>2</sub> from <em>Mycobacterium avium</em> is a mixture of two homologous peptidoglycolipids: C<sub>2a</sub> (Formula III) and C<sub>2b</sub> (Formula IV). Both have the following partial structure: <figure><img></figure></p><p>The C-terminal molecule of <span>D</span>-alanine esterifies the pseudoaldehydic function of 6-deoxy-<span>L</span>-talose in mycoside C<sub>2a</sub> and that of <span><math><mtext>3-O-</mtext><mtext>methyl-6-deoxy-</mtext><mtext>L</mtext><mtext>-talose</mtext></math></span> in mycoside C<sub>2b</sub>. One acetyl group is linked to 3,4-di-<em>O</em>-methylrhamnose and two acetyl groups to 6-deoxy-<span>L</span>-talose and to <span><math><mtext>3-O-</mtext><mtext>methyl-6-deoxy-</mtext><mtext>L</mtext><mtext>-talose</mtext></math></span>.</p><p>the lipid moiety is an acid (or mixture of acids) RCOOH of approximate formula C<sub>43</sub>H<sub>86</sub>O<sub>2</sub> ± 3 CH<sub>2</sub>, or C<sub>46</sub>H<sub>90</sub>O<sub>3</sub> ± 3 CH<sub>2</sub> bound by an amide linkage to the amino group of <span>D</span>-phenylalanine.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91643-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23683021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Effects of hydrocortisone on the synthesis of sulphated mucopolysaccharides and collagen in chick embryos 氢化可的松对鸡胚硫酸粘多糖和胶原蛋白合成的影响
Biochimica et biophysica acta Pub Date : 1963-10-29 DOI: 10.1016/0006-3002(63)91656-1
Paul S. Ebert , Darwin J. Prockop
{"title":"Effects of hydrocortisone on the synthesis of sulphated mucopolysaccharides and collagen in chick embryos","authors":"Paul S. Ebert ,&nbsp;Darwin J. Prockop","doi":"10.1016/0006-3002(63)91656-1","DOIUrl":"10.1016/0006-3002(63)91656-1","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91656-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23682789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 21
Isolement de fractions peptido-glycolipidiques à partir des cires d de mycobactéries bovines, atypiques, aviaires et saprophytes 从牛、非典型、家禽和腐生分枝杆菌d蜡中分离肽-糖脂组分
Biochimica et biophysica acta Pub Date : 1963-10-29 DOI: 10.1016/0006-3002(63)91644-5
P. Jollès, D. Samour, E. Lederer
{"title":"Isolement de fractions peptido-glycolipidiques à partir des cires d de mycobactéries bovines, atypiques, aviaires et saprophytes","authors":"P. Jollès,&nbsp;D. Samour,&nbsp;E. Lederer","doi":"10.1016/0006-3002(63)91644-5","DOIUrl":"10.1016/0006-3002(63)91644-5","url":null,"abstract":"<div><p>By fractional ultracentrifugation in ether it was possible to isolate peptidoglycolipid fractions from wax D of atypical, avian and saprophytic strains. The “heavy” fractions from wax D of the atypical strain No. 4 (<em>Mycobacterium kansasii</em>), of <em>Mycobacterium avium</em> and of <em>Mycobacterium phlei</em> contain a peptide moiety with alanine, glutamic acid and <em>meso</em>-<em>α</em>,<em>α</em>′-diaminopimelic acid in approximate molar ratio 3:2:2, similar to that isolated from wax D fractions of human strains. By mass spectrometry it was shown that the mycolic acids of wax D from the atypical photochromogenic strain N. 4 (<em>M. kansasii</em>) are of the “saprophytic” type.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91644-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23683022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
Final proof for the identity of enzymic specificities of egg-white lysozyme and phage T2 enzyme 蛋白溶菌酶和噬菌体T2酶特异性鉴定的最终证明
Biochimica et biophysica acta Pub Date : 1963-10-29 DOI: 10.1016/0006-3002(63)91648-2
D. Maass, W. Weidel
{"title":"Final proof for the identity of enzymic specificities of egg-white lysozyme and phage T2 enzyme","authors":"D. Maass,&nbsp;W. Weidel","doi":"10.1016/0006-3002(63)91648-2","DOIUrl":"10.1016/0006-3002(63)91648-2","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91648-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23682782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Further studies on the incorporation of d-methionine into the bacterial cell wall d-蛋氨酸进入细菌细胞壁的进一步研究
Biochimica et biophysica acta Pub Date : 1963-10-29 DOI: 10.1016/0006-3002(63)91638-X
C. Lark , D. Bradley , K.G. Lark
{"title":"Further studies on the incorporation of d-methionine into the bacterial cell wall","authors":"C. Lark ,&nbsp;D. Bradley ,&nbsp;K.G. Lark","doi":"10.1016/0006-3002(63)91638-X","DOIUrl":"10.1016/0006-3002(63)91638-X","url":null,"abstract":"<div><p><span>d</span>-Methionine is incorporated into the cell walls of <em>Alcaligenes fecalis</em> thereby blocking further synthesis of this stucture. In the present paper evidence is presented demonstrating incorporation of <span>d</span>-methionine into a component of the phenol-insoluble cell wall which may be liberated by lysozyme (<em>N</em>-acetylmuramide glycanohydrolase, EC 3.2.1.17). Chromatography of the products of lysozyme digestion indicate that <span>d</span>-methionine is incorporated into a complex structure, which can be broken into different units by lysozyme. Analysis has shown that neither the amino nor the carboxyl groups of methionine are free when this amino acid is incorporated into the cell wall. Electron-microscopic studies have revealed lesions in the wall of cells grown in the presence of <span>d</span>-methionine as well as the liberation of firbous material from the cell wall. This fibrous material appears to be lysozyme sensitive and may possibly originate from a more compact subunit.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91638-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23683016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Characterization of the hypobranchial mucin on the whelk Buccinum undatum L. 棘海螺鳃下黏液蛋白的表征。
Biochimica et biophysica acta Pub Date : 1963-10-29 DOI: 10.1016/0006-3002(63)91651-2
S. Hunt, F.R. Jevons
{"title":"Characterization of the hypobranchial mucin on the whelk Buccinum undatum L.","authors":"S. Hunt,&nbsp;F.R. Jevons","doi":"10.1016/0006-3002(63)91651-2","DOIUrl":"10.1016/0006-3002(63)91651-2","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91651-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23682785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Heterogeneity of the carbohydrate moiety of crystalline ovalbumin 结晶卵清蛋白碳水化合物部分的异质性
Biochimica et biophysica acta Pub Date : 1963-10-29 DOI: 10.1016/0006-3002(63)91652-4
Leon W. Cunningham, R. Wilburn Clouse, John D. Ford
{"title":"Heterogeneity of the carbohydrate moiety of crystalline ovalbumin","authors":"Leon W. Cunningham,&nbsp;R. Wilburn Clouse,&nbsp;John D. Ford","doi":"10.1016/0006-3002(63)91652-4","DOIUrl":"10.1016/0006-3002(63)91652-4","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91652-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23682786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 31
Incorporation of d-[14C]glucose into individual gangliosides d-[14C]葡萄糖与单个神经节苷的结合
Biochimica et biophysica acta Pub Date : 1963-10-29 DOI: 10.1016/0006-3002(63)91655-X
Kunihiko Suzuki, Saul R. Korey
{"title":"Incorporation of d-[14C]glucose into individual gangliosides","authors":"Kunihiko Suzuki,&nbsp;Saul R. Korey","doi":"10.1016/0006-3002(63)91655-X","DOIUrl":"10.1016/0006-3002(63)91655-X","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91655-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23682788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
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