African health sciences最新文献

{"title":"The Luganda Edinburgh Postnatal depression scale: cross-cultural adaptation and validation for prenatal screening of depression in a Ugandan sample.","authors":"David Christopher Mukasa, Sam Ononge, Imelda Namagembe, Josaphat Byamugisha, Musa Sekikubo, Mark Muyingo, Noeline Nakasujja","doi":"10.4314/ahs.v24i4.28","DOIUrl":"10.4314/ahs.v24i4.28","url":null,"abstract":"<p><strong>Background: </strong>Depression affects approximately 364 million people globally. Prenatal depression affects between 26.3% and 32.9% of mothers in Africa. Opportunities for prenatal screening are missed. The gold standard diagnostic, the Diagnostic and Statistical Manual of Mental Disorders-Fifth edition (DSM-5) criteria for Major Depressive Disorder (MDD) has higher technical requirement. There is inadequate information on locally adapted and validated user-friendly screening tools in Uganda.</p><p><strong>Objective: </strong>To Adapt and validate the Luganda Edinburgh Postnatal Depression Scale (EPDS-L) for screening prenatal depression at Kawempe National Referral hospital (KNRH).</p><p><strong>Methods: </strong>Cross-sectional study in KNRH using International Society for Pharmacoeconomics and Outcomes Research guidelines for adaptation and quantitative approaches for the validation. Consecutive sampling until the desired sample of 100, all participants responded to both EPDS-L and DSM-5 criteria for MDD. Reliability demonstrated using Cronbach's alpha coefficient, while validity was demonstrated by sensitivity, specificity, Negative Predictive Value (NPV), Positive Predictive Value (PPV) and Area-Under-the-curve (AUC).</p><p><strong>Results: </strong>EPDS-L had Cronbach's-Alpha of 0.8515. At cut-off of 13, sensitivity was 62.86%, specificity-100%, PPV-100% and NPV-83.3%. AUC was 0.99. Performance was better at cut-off of 10, with sensitivity-97.14% and specificity-98.46%.</p><p><strong>Conclusion: </strong>The EPDS-L is reliable at cut-off of 13 but performs even better at cut-off of 10.</p>","PeriodicalId":94295,"journal":{"name":"African health sciences","volume":"24 4","pages":"214-223"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preventive measures of obstetric Fistula: knowledge and practice among service providers in two Nigerian Health Institutions.","authors":"Chikaodili Ndidiamaka Ihudiebube-Splendor, Nonyelum Nnenna Jisieike-Onuigbo, Paulina Chigwara Chikeme, Onyinyechi Lilian Utazi, Anulika Jennifer Nnamani","doi":"10.4314/ahs.v24i4.22","DOIUrl":"10.4314/ahs.v24i4.22","url":null,"abstract":"<p><strong>Background: </strong>Obstetric fistula remains a major public health problem which serves as a proxy indicator of the status of Nigerian women and of the availability and accessibility to quality maternal health services. This study aims to assess the knowledge, practice and perceived factors that might hinder preventive interventions to reduce obstetric fistula by service providers in Abakaliki, Ebonyi State Nigeria.</p><p><strong>Methods: </strong>A cross-sectional survey of 169 service providers (doctors (49) and nurses (120)) in selected health facilities in Abakaliki was conducted using a validated self-administered questionnaire.</p><p><strong>Results: </strong>Majority (71%) of the participants were registered nurse/midwife while only 29% were doctors. Most (87.0% & 89.9%) of the participants had both adequate knowledge and good practice of preventive measures of obstetric fistula respectively. Major factors perceived to hider the practice of obstetric fistula preventive measures were patient-related factors (mean=2.97) and institutional factors (mean = 2.51). There was significant association between age (p = 0.008), marital status (p = 0.029), profession (p = 0.039), years of experience of work (p = 0.003) and the knowledge of obstetric fistula while none of the demographic characteristics had association (p > 0.05) with the practice of obstetric fistula preventive measures.</p><p><strong>Conclusion: </strong>Although most participants in this study had adequate knowledge and good practice of obstetric fistula preventive measures, there is still a great need to train and retrain doctors and nurses on current guidelines for obstetric fistula prevention and conservative management. Government should also strengthen the health facilities at all levels to provide emergency obstetric and newborn care.</p>","PeriodicalId":94295,"journal":{"name":"African health sciences","volume":"24 4","pages":"163-173"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effectiveness of COVID-19 Heterologous Vaccination: the experience from a Regional Hospital in Cameroon.","authors":"Princewill Kum Unji, Alex Mambap Tatang, Samuel Angwafor, Loveline Lum Niba, Adji Minette Jaqueline Porro, Denis Nsame Nforniwe, Andreas Chiabi","doi":"10.4314/ahs.v24i4.11","DOIUrl":"10.4314/ahs.v24i4.11","url":null,"abstract":"<p><strong>Background: </strong>With most COVID-19 vaccines requiring at least 2 doses, heterologous vaccination will facilitate vaccination programs where vaccine supplies fluctuate. However, with insufficient data on effects of heterologous vaccination in our setting, people remain reluctant to mix.</p><p><strong>Objectives: </strong>We seek to assess the effects of heterologous vaccination on morbidity and mortality.</p><p><strong>Methods: </strong>This was a 7 months retrospective study for COVID-19 patients managed by the Bamenda Regional Hospital, Cameroon, running from the 18th August 2021 to 28^th February 2022. Logistic regression used to asses relationship between predictors and outcome.</p><p><strong>Results: </strong>Our 1254 participants had a mean age of 50.1(±19.6) years, we had 24 (1.9%) being fully vaccinated, where 7 (29.2%) took heterologous vaccines. Also, 721 (57.5%) had the moderate/severe form of the disease. Those fully vaccinated had lower odds of having severe disease (p=0.037). However, heterologous vaccination compared to homologous vaccination had no significant difference on morbidity (p=0.729). Among patients who died, none was fully vaccinated.</p><p><strong>Conclusion: </strong>The protective effect of vaccination on morbidity was similar in those with heterologous vaccination as well as those who took.</p>","PeriodicalId":94295,"journal":{"name":"African health sciences","volume":"24 4","pages":"85-90"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and pattern of renal complications of uterine fibroids in a Teaching Hospital in Nigeria.","authors":"Mumuni Amisu, Joy Chionuma, Ayoola Odeyemi, Odewale Odetayo","doi":"10.4314/ahs.v24i4.21","DOIUrl":"10.4314/ahs.v24i4.21","url":null,"abstract":"<p><strong>Background: </strong>Symptomatic uterine fibroids causing obstructive nephropathy were thought to be uncommon, but recent prevalence studies showed otherwise. The aim of the study is to determine the prevalence of obstructive uropathy and nephropathy in patients with uterine leiomyoma.</p><p><strong>Methodology: </strong>The study was a hospital-based cross-sectional retrospective study of 114 patients diagnosed with symptomatic uterine fibroids seen in the gynecology clinic of Lagos State University Teaching Hospital, Ikeja from February 2021 to June 2022.</p><p><strong>Results: </strong>The overall prevalence of obstructive uropathy and nephropathy was 22.8% and 10.5% respectively. The average level of serum creatinine was 2.2 ± 1.9mg/dl with a creatinine range of 1.8 to 6.7mg dl. The average of the GFR among patients was 29.3 ± 13.4ml/min/1.73m² with a range of 9 - 54ml/min/1.73m². There is a positive association between uterine size and uropathy and subjects with uropathy are more likely to develop nephropathy. (CI 95% 3.281 - 108.670).</p><p><strong>Conclusion: </strong>This study showed that prevalence of obstructive nephropathy in the patients with uterine leiomyomata is 10.5%. Uterine size and myoma diameter were positively associated with development of obstructive nephropathy.</p>","PeriodicalId":94295,"journal":{"name":"African health sciences","volume":"24 4","pages":"156-162"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fertility outcome of intravaginal insemination in women with unconsummated marriages.","authors":"Rubina Izhar, Samia Husain, Zubaida Masood","doi":"10.4314/ahs.v24i4.18","DOIUrl":"10.4314/ahs.v24i4.18","url":null,"abstract":"<p><strong>Background: </strong></p><p><strong>Aim: </strong>To assess the fertility outcome of intravaginal insemination in women with unconsummated marriages.</p><p><strong>Methods: </strong>We conducted a prospective observational study from January 2017 to October 2020. The study population comprised of couples with unconsummated marriages. Participants were divided into three groups: (1) women with vaginismus (2) men with erectile dysfunction(ED); and (3) men who suffered from premature ejaculation (PE). The couple was educated about the fertility period and advised to perform insemination at home on every alternate day for at least twelve cycles. Pregnancy was defined as a positive urine pregnancy test. The couple was asked to report a positive pregnancy test or follow up at the end of twelve months in case conception did not occur.</p><p><strong>Results: </strong>The study comprised of 769 couples. There were 371 couples with vaginismus, 258 with premature ejaculation and 140 with erectile dysfunction. Pregnancy was achieved in 270(72.8%) couples with vaginismus, 181(70.2%) with premature ejaculation and 72(51.4%) with erectile dysfunction. The time to conception was shortest for couples with vaginismus, 7.72±3.02 months and longest for couples with erectile dysfunction 10.81±1.53 months.</p><p><strong>Conclusion: </strong>Couples with unconsummated marriages conceive with intravaginal insemination. At home insemination is a treatment option for such couples before embarking on artificial reproductive techniques.</p>","PeriodicalId":94295,"journal":{"name":"African health sciences","volume":"24 4","pages":"137-142"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interaction between CTGF and VEGF-A in the progression of intervertebral disc fibrosis.","authors":"Wangbing Xu, Jiqin Zhong, Jianrong Jian, Faming Zhong","doi":"10.4314/ahs.v24i4.36","DOIUrl":"10.4314/ahs.v24i4.36","url":null,"abstract":"<p><strong>Background: </strong>Fibrosis in the extracellular matrix of nucleus pulposus (NP) is associated with intervertebral disc degeneration (IVDD). Both connective tissue growth factor (CTGF) and vascular endothelial growth factor (VEGF)-A are responsible for the pathological basis of NP fibrosis. Our study aims to verify the interaction between CTGF and VEGF-A in a vitro NP cell model.</p><p><strong>Methodology: </strong>Collected human NP tissues of different degeneration degree and isolated the NP cells from the non-degenerated NP tissues. Analysed the CTGF and VEGF-A gene expression in the naturally degenerated NP and IL-1β-induced degenerated NP cells. Additionally, interfered wit the CTGF and VEGF-A expression by exogenic protein treatment, siRNA transfection, or specific inhibitor. The expression of CTGF, VEGF-A, collagen I/II/III and aggrecan with protein or mRNA level was determined by immunological staining, western blotting and RT-PCR.</p><p><strong>Results: </strong>CTGF and VEGF-A highly expressed in the late-term of degeneration compared to the middle-term, and their expressions were synergistic. Upregulating one of CTGF and VEGF-A could induce the overexpression of the other one and collagen I/III, but suppressed collagen II and aggrecan expression; Besides, the suppression of one of them could inhibited another and collagen I/III expression.</p><p><strong>Conclusions: </strong>CTGF and VEGF-A increase in late IVDD. Prevent NP fibrosis by suppressing their interaction.</p>","PeriodicalId":94295,"journal":{"name":"African health sciences","volume":"24 4","pages":"276-285"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The predictive prognostic value of serum CEA, CA153, HE4 combined with a variety of immune inflammatory indicators in breast cancer.","authors":"Ping Zou, Ke Li, Yiting Qian, Tingting Wu, Yue Qian","doi":"10.4314/ahs.v24i4.29","DOIUrl":"10.4314/ahs.v24i4.29","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the prognostic value of serum carcinoembryonic antigen (CEA), carbohydrate antigen (CA153), and human epididymal 4 (HE4) combined with a variety of immune inflammatory indicators in breast cancer.</p><p><strong>Methods: </strong>A sample size of 112 breast cancer patients was selected and all patients underwent surgical treatment. The three-year prognosis of the patients was observed. Patients with poor prognoses were included in the poor prognoses group and the patients with good prognosis were included in the good prognosis group. The levels of serum CEA, CA153, HE4, and related immune inflammatory indicators (including Mon, Lym, Neu, Plt, NLR, and SII) were analysed. The predictive value of serum CEA, CA153, HE4, and immune inflammatory indicators on the prognosis of breast cancer was analysed by Logistic regression and ROC curve method. The area under the ROC curve (AUC) was used to evaluate the predictive efficiency. α=0.05 was used as the test standard.</p><p><strong>Results: </strong>Compared with the good prognosis group, the CEA, CA153, HE4, Mon, Neu, Plt, and SII of the poor prognosis group were higher, all P<0.05. Logistic regression analysis showed that CEA, CA153, HE4, Neu, and Plt were effective indicators for predicting the poor prognosis of breast cancer. The AUC of CEA, CA153, HE4, Neu, and Plt in predicting the prognosis of breast cancer was 0.929, which was higher than the AUC of a single prediction.</p><p><strong>Conclusion: </strong>Serum CEA, CA153, HE4 combined with Neu and Plt had predictive value for the prognosis of breast cancer.</p>","PeriodicalId":94295,"journal":{"name":"African health sciences","volume":"24 4","pages":"224-232"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of diversity of fetal heart function in pregnancy-induced hypertension patients by ultrasonography in Aljouf Region, Saudi Arabia.","authors":"Md Sayed Ali Sheikh, A Alduraywish, Muhannad Faleh Alanazi, Ahmed Baker A Alshaikh, Umme Salma","doi":"10.4314/ahs.v24i4.25","DOIUrl":"10.4314/ahs.v24i4.25","url":null,"abstract":"<p><strong>Background: </strong>The common pregnancy illness known as pregnancy-induced hypertension syndrome (PIH) has the potential to harm both the mother's and the fetus' organs to varying degrees. An essential tool for assessing fetal heart function is ultrasound.</p><p><strong>Objective: </strong>The goal of this study is to use ultrasound to measure the variety of fetal heart function in patients with pregnancy-induced hypertension.</p><p><strong>Material and methods: </strong>This hospital-based retrospective study was carried out between January 2021 and January 2022 at the Obstetric Department of Maternity and Children Hospital (MCH), Sakaka, Aljouf, Saudi Arabia, to assess the changes in fetal cardiac function in patients with pregnancy-induced hypertension compared to healthy pregnant women in general. The research process involved screening 60 pregnant women for pregnancy-induced hypertension (PIH) and 40 healthy pregnant women for the control group. Each of the 100 pregnant women had a single fetus, and no prenatal abnormalities were found. All eligible patients underwent prenatal ultrasounds in order to collect the cardiac function measures needed to assess the variety of fetal heart functions rapidly and effectively.</p><p><strong>Results: </strong>When compared to healthy pregnant women, the results of the ultrasound show a higher significance of the fetal thickness of the cardiac septum, left and right ventricular end-systolic and end-diastolic perimeter and area. Additionally, our ultrasonography findings demonstrate that fetal ventricular systolic fractions 1 and 2 (VSF1 and VSF2) were more significant in patients with pregnancy-induced hypertension (PIH) compared to healthy pregnant women in general.</p><p><strong>Conclusion: </strong>The cardiac functions of the fetus are negatively affected by pregnancy-induced hypertension (PIH).</p>","PeriodicalId":94295,"journal":{"name":"African health sciences","volume":"24 4","pages":"192-197"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of general anesthesia combined with ultrasound-guided lumbosacral plexus block on hemodynamics and postoperative rehabilitation in geriatric patients with total hip arthroplasty.","authors":"Yanan Cui, Qin Fan, Na Wu, Baichun Xing","doi":"10.4314/ahs.v24i4.38","DOIUrl":"10.4314/ahs.v24i4.38","url":null,"abstract":"<p><strong>Background: </strong>The incidence of fractures increases with age, and it is estimated that the incidence in women is higher than that in men.</p><p><strong>Objective: </strong>To investigate the influence of general anesthesia & ultrasound-guided lumbosacral plexus block in elderly patients with total hip arthroplasty (THA).</p><p><strong>Methods: </strong>Eighty-one geriatric sufferers who were sent to our hospital from July 2019 to January 2021 and underwent THA surgery in the Department of Orthopedics were divided into Grades I to III according to American Society of Anesthesiologists (ASA). Forty-one patients were ascribed to a combination group (using general anesthesia combined with guided lumbosacral plexus block), and forty to a general anesthesia group (with general anesthesia) by a random number table to compare the differences in mean arterial pressure (MAP), heart rate (HR), pulse oxygen saturation (SpO2), intraoperative general anesthesia dosage, incidence of postoperative delirium (POD), serum inflammation and oxidative stress indexes between them.</p><p><strong>Results: </strong>Prior to anesthesia induction, MAP, HR, and SpO2 between them had significant differences (P>0.05); at skin-incision-time and implantation of the prosthesis, the MAP value of the experimental group was higher than that of the general anesthesia group, 30 minutes after surgery, the SpO2 was higher than the general anesthesia group, and had significant differences (P<0.05); prior to the surgery, had significant differences in measured values such as Cor, blood glucose, IL-6, CRP, MDA and SOD between the two groups (P>0.05); 2 hours after that, the values of Cor, CRP, and MDA in experimental group were lower than the general anesthesia group, and that of IL-6 and SOD were higher than those in the general anesthesia group, and had significant differences (P<0.05); the dosage of propofol, remifentanil, sufentanil in experimental group were lower than the general anesthesia group, while postoperative extubation time in experimental group was earlier than general anesthesia group, and had significant differences (P<0.05); the incidence of POD in experimental group was lower than the general anesthesia group at 1d, 2d, and 3d after surgery, and had significant differences (P<0.05); the incidence of adverse reactions of anesthesia in experimental group was lower than the general anesthesia group with 9.76 % to 27.50 %, and had significant differences (P<0.05).</p><p><strong>Conclusion: </strong>General anesthesia & ultrasound-guided lumbosacral plexus block in THA patients is more conducive to the patients' hemodynamic stability, reducing inflammatory stress response, the dosage of anesthetic drugs, and the incidence of POD, compared with general anesthesia alone.</p>","PeriodicalId":94295,"journal":{"name":"African health sciences","volume":"24 4","pages":"299-307"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro scientific validation of anti-inflammatory activity of <i>Punica granatum L.</i> on Leukemia monocytic cell line.","authors":"Sharmistha Dutta, Khushbu Nishad, Talambedu Usha, Nijalingappa Ramesh, Sushil Kumar Middha","doi":"10.4314/ahs.v24i4.31","DOIUrl":"10.4314/ahs.v24i4.31","url":null,"abstract":"<p><strong>Background: </strong>The induction of the inflammatory cascade results in the production of a number of inflammatory mediators, including prostaglandin E2 (PGE2), nitric oxide (NO), and proinflammatory cytokines like TNF-, IL-, and IL-6. This study examined the cytotoxicity and anti-inflammatory properties of a methanolic crude extract of Punica granatum L. peel (PPM) on monocytic leukaemia cell line (THP-1).</p><p><strong>Materials and methods: </strong>The PPM along with Quercetin as reference was used to assess the cytotoxic effect on THP-1 cells and describe its effect on pro-inflammatory cytokines such as COX-2, TNF-α, IL-6 against cancer cell line by flow cytometry.</p><p><strong>Results: </strong>The percentage of viable cells significantly decreased which correlates to non-toxicity whereas quercetin was found to be highly toxic, the IC50 could not be calculated because of drug precipitation. There was a significant decrease in the expressions of inflammatory cytokines upon pre-treatment of the cells with PPM prior to LPS stimulation.</p><p><strong>Conclusion: </strong>Our findings indicate that no cytotoxicity was observed after the treatment of THP-1 cells with PPM (25-400 µg/ml), but at higher concentration (400µg/ml), the cell viability decreased to 84% and attenuated the expression level of inflammatory cytokines. The inhibitory effect of the extract on pro-inflammatory factors production may provide a theoretical source on upcoming treatment of inflammation.</p>","PeriodicalId":94295,"journal":{"name":"African health sciences","volume":"24 4","pages":"240-249"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970145/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}