Zebrafish最新文献_第6页

Laboratory Course Using Zebrafish to Uncover Changing Roles of Wnt Signaling in Early Vertebrate Development. 利用斑马鱼揭示 Wnt 信号在脊椎动物早期发育中不断变化的作用的实验课程。

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0070

Manuel Fiegl, Robin A Kimmel

引用次数: 0

A 3D-Printed and Freely Available Device to Measure the Zebrafish Optokinetic Response Before and After Injury. 用于测量斑马鱼受伤前后视运动反应的三维打印和免费提供的装置。

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0071

Ashley Hermans, Sophia Tajnai, Allison Tieman, Sarah Young, Ashley Franklin, Mackenzie Horutz, Steven J Henle

{"title":"A 3D-Printed and Freely Available Device to Measure the Zebrafish Optokinetic Response Before and After Injury.","authors":"Ashley Hermans, Sophia Tajnai, Allison Tieman, Sarah Young, Ashley Franklin, Mackenzie Horutz, Steven J Henle","doi":"10.1089/zeb.2023.0071","DOIUrl":"10.1089/zeb.2023.0071","url":null,"abstract":"Zebrafish eyes are anatomically similar to humans and have a higher percentage of cone photoreceptors more akin to humans than most rodent models, making them a beneficial model organism for studying vision. However, zebrafish are different in that they can regenerate their optic nerve after injury, which most other animals cannot. Vision in zebrafish and many other vertebrate animals, including humans, can be accessed using the optokinetic response (OKR), which is an innate eye movement that occurs when tracking an object. Because fish cannot use an eye chart, we utilize the OKR that is present in virtually all vertebrates to determine if a zebrafish has vision. To this end, we have developed an inexpensive OKR setup that uses 3D-printed and off-the-shelf parts. This setup has been designed and used by undergraduate researchers and is also scalable to a classroom laboratory setup. We demonstrate that this setup is fully functional for assessing the OKR, and we use it to illustrate the return of the OKR following optic nerve injury in adult zebrafish.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"21 2","pages":"144-148"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11035845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140871831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Teaching Zebrafish Development in a STEM-Based Science Camp for Middle School Students. 在以 STEM 为基础的中学生科学营中教授斑马鱼的发育。

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0082

T. Franz-Odendaal, Juan D. Carvajal-Agudelo, Sally Marchand

引用次数: 0

β-Estradiol Supplementation Regulates Cholesterol Synthesis Independent of Unsaturated Fat Consumption in Adult Zebrafish. 补充β-雌二醇可调节成年斑马鱼的胆固醇合成，与不饱和脂肪摄入无关

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0066

Rachel V Yinger, Colin J Evangelisto, Nicholas C Tucker, Jessica M Billingsley, Samantha L St Clair

引用次数: 0

Exploring the Hawaiian Ala Wai Watershed with Zebrafish. 用斑马鱼探索夏威夷阿拉威流域。

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0092

Zachary Oglesby, Alanna Nicole Rillorta, Cheydon Agos, Ku'uipo Borges, Saien Cabradilla, Michael Garvin, Bryn Higuchi, Elisabeth Kamaka, Chancen Law, Matthew Liu, Grace Matsumoto, Tiffany Ng, Gemma Quiroz, Chelsea Ramiro, Jamie Saito, Malia Williams, Asia Yamada, Zane Yogi, Sidney Olson, Soaleha Shams, K. Withy, Christopher Pierret

{"title":"Exploring the Hawaiian Ala Wai Watershed with Zebrafish.","authors":"Zachary Oglesby, Alanna Nicole Rillorta, Cheydon Agos, Ku'uipo Borges, Saien Cabradilla, Michael Garvin, Bryn Higuchi, Elisabeth Kamaka, Chancen Law, Matthew Liu, Grace Matsumoto, Tiffany Ng, Gemma Quiroz, Chelsea Ramiro, Jamie Saito, Malia Williams, Asia Yamada, Zane Yogi, Sidney Olson, Soaleha Shams, K. Withy, Christopher Pierret","doi":"10.1089/zeb.2023.0092","DOIUrl":"https://doi.org/10.1089/zeb.2023.0092","url":null,"abstract":"The Ala Wai Canal is an artificial waterway in the tourist district of Waikiki in Honolulu, HI. Originally built to collect runoff from industrial, residential, and green spaces dedicated to recreation, the Ala Wai Canal has since experienced potent levels of toxicity due to this runoff entering the watershed and making it hazardous for both marine life and humans at current concentration, including Danio rerio (zebrafish). A community of learners at educations levels from high school to postbaccalaureate from Oahu, HI was connected through the Consortium for Increasing Research and Collaborative Learning Experiences (CIRCLE) distance research program. This team conducted research with an Investigator and team from Mayo Clinic in Rochester, MN, with the Ala Wai Canal as its primary subject. Through CIRCLE, research trainees sent two 32 oz bottles of Ala Wai- acquired water to a partnered laboratory at the Mayo Clinic in which zebrafish embryos were observed at differing concentrations of the sampled water against a variety of developmental and behavioral assays. Research trainees also created atlases of developmental outcomes in zebrafish following exposure to environmental toxins and tables of potential pesticide contaminants to enable the identification of the substances linked to structural defects and enhanced stress during Ala Wai water exposure experiments.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"363 ","pages":"206-213"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140778435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Teaching Fin Regeneration Using a Dominant Negative Receptor. 利用显性阴性受体进行鳍再生教学

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0062

Greta M Mahler, Barbara E Sisson

引用次数: 0

Whole-Mount Histochemical Protocols in Zebrafish for a Hands-On Developmental Biology Practical Course in Ecuador. 为厄瓜多尔的发育生物学实践课程提供斑马鱼全图组织化学方法。

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0076

Ariel Arévalo Cuaical, Estibaliz Maitea Villaverde, Daniela Zurita-Paredes, Erika Samary Pinto-Nogales, Gabriela Bautista, Josselyn Calero, Sharon Moreira, Daniela Rosero, Felipe Varela, Andrés Romero-Carvajal

{"title":"Whole-Mount Histochemical Protocols in Zebrafish for a Hands-On Developmental Biology Practical Course in Ecuador.","authors":"Ariel Arévalo Cuaical, Estibaliz Maitea Villaverde, Daniela Zurita-Paredes, Erika Samary Pinto-Nogales, Gabriela Bautista, Josselyn Calero, Sharon Moreira, Daniela Rosero, Felipe Varela, Andrés Romero-Carvajal","doi":"10.1089/zeb.2023.0076","DOIUrl":"https://doi.org/10.1089/zeb.2023.0076","url":null,"abstract":"Research-based education at the undergraduate level is ideal for fostering the training of future scientists. In an undergraduate Developmental Biology course, this learning strategy requires the availability of model species and enough research reagents, not only for technique training but also for the development of student original projects. This might be challenging in most countries, where resources are limited. Hence, there is a need to develop low-cost solutions for use in the classroom. In this study, we describe the optimization and use of two low-cost protocols in zebrafish embryos for hands-on practical sessions and project-based learning in a Developmental Biology undergraduate course in Ecuador. These protocols were designed for the practical and experimental learning of vertebrate meroblastic cleavage, gastrulation, and neural crest differentiation. The proposed protocols have been previously described in the literature and use silver nitrate and alcian blue, two relatively inexpensive reagents, to label cell membranes and cartilage. The silver nitrate protocol allows the study of cell contact formation during cleavage and the identification of cellular changes during gastrulation, including yolk internalization and epiboly. The alcian blue staining allows the analysis of cranial mesenchymal differentiation into cartilage. These protocols are ideal for practical sessions due to their ease of application, quick results, adaptability to the class schedule, and robustness in the hands of beginning researchers. Finally, these protocols are adaptable for research-based class projects.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"81 3","pages":"119-127"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140792701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alternative Splicing and Nonsense-Mediated Decay of a Zebrafish GABA Receptor Subunit Transcript. 斑马鱼GABA受体亚单位转录的选择性剪接和无义介导的衰变。

Zebrafish Pub Date : 2024-04-01 Epub Date: 2023-09-25 DOI: 10.1089/zeb.2023.0044

Gaia J Moisan, Nitika Kamath, Shannon Apgar, Megan Schwehr, Pooja Vedmurthy, Olivya Conner, Kyler Hayes, Cecilia Phillips Toro

引用次数: 0

Differential Roles of Diet on Development and Spinal Cord Regeneration in Larval Zebrafish. 饮食对幼体斑马鱼发育和脊髓再生的不同作用

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0042

Emily J Purifoy, Karen Mruk

引用次数: 0

Effects of 4 Testing Arena Sizes and 11 Types of Embryo Media on Sensorimotor Behaviors in Wild-Type and chd7 Mutant Zebrafish Larvae. 4种测试场大小和11种胚胎培养基对野生型和chd7突变型斑马鱼幼体感官运动行为的影响

Zebrafish Pub Date : 2024-02-01 DOI: 10.1089/zeb.2023.0052

Dana R Hodorovich, Tiara Fryer Harris, Derek F Burton, Katie M Neese, Rachael A Bieler, Vimal Chudasama, Kurt C Marsden

{"title":"Effects of 4 Testing Arena Sizes and 11 Types of Embryo Media on Sensorimotor Behaviors in Wild-Type and chd7 Mutant Zebrafish Larvae.","authors":"Dana R Hodorovich, Tiara Fryer Harris, Derek F Burton, Katie M Neese, Rachael A Bieler, Vimal Chudasama, Kurt C Marsden","doi":"10.1089/zeb.2023.0052","DOIUrl":"10.1089/zeb.2023.0052","url":null,"abstract":"The larval zebrafish is a highly versatile model across research disciplines, and the expanding use of behavioral analysis has contributed to many advances in neuropsychiatric, developmental, and toxicological studies, often through large-scale chemical and genetic screens. In the absence of standardized approaches to larval zebrafish behavior analysis, however, it is critical to understand the impact on behavior of experimental variables such as the size of testing arenas and the choice of embryo medium. Using a custom-built, modular high-throughput testing system, we examined the effects of 4 testing arena sizes and 11 types of embryo media on conserved sensorimotor behaviors in zebrafish larvae. Our data show that testing arena size impacts acoustic startle sensitivity and kinematics, as well as spontaneous locomotion and thigmotaxis, with fish tested in larger arenas displaying reduced startle sensitivity and increased locomotion. We also find that embryo media can dramatically affect startle sensitivity, kinematics, habituation, and prepulse inhibition, as well as spontaneous swimming, turning, and overall activity. Common medium components such as methylene blue and high calcium concentration consistently reduced startle sensitivity and locomotion. To further address how the choice of embryo medium can impact phenotype expression in zebrafish models of disease, we reared chd7 mutant larvae, a model of CHARGE syndrome with previously characterized morphological and behavioral phenotypes, in five different types of media and observed impacts on all phenotypes. By defining the effects of these key extrinsic factors on larval zebrafish behavior, these data can help researchers select the most appropriate conditions for their specific research questions, particularly for genetic and chemical screens.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10902501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139674011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0