Zebrafish最新文献_第5页

Bringing Real Inquiry-Based Science to Diverse Secondary Educational Environments: A Virtual Zebrafish Laboratory to Investigate Environmental Health. 将真正的探究式科学引入多样化的中学教育环境：研究环境健康的虚拟斑马鱼实验室。

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0087

M. Carvan, Thomas Hansen, Renee A. Hesselbach, Amy Zientek, Craig A. Berg, David H. Petering

{"title":"Bringing Real Inquiry-Based Science to Diverse Secondary Educational Environments: A Virtual Zebrafish Laboratory to Investigate Environmental Health.","authors":"M. Carvan, Thomas Hansen, Renee A. Hesselbach, Amy Zientek, Craig A. Berg, David H. Petering","doi":"10.1089/zeb.2023.0087","DOIUrl":"https://doi.org/10.1089/zeb.2023.0087","url":null,"abstract":"The goal of the University of Wisconsin-Milwaukee WInSTEP SEPA program is to provide valuable and relevant research experiences to students and instructors in diverse secondary educational settings. Introducing an online experience allows the expansion of a proven instructional research program to a national scale and removes many common barriers. These can include lack of access to zebrafish embryos, laboratory equipment, and modern classroom facilities, which often deny disadvantaged and underrepresented students from urban and rural school districts valuable inquiry-based learning opportunities. An online repository of zebrafish embryo imagery was developed in the Carvan laboratory to assess the effects of environmental chemicals. The WInSTEP SEPA program expanded its use as an accessible online tool, complementing the existing classroom experience of our zebrafish module. This virtual laboratory environment contains images of zebrafish embryos grown in the presence of environmental toxicants (ethanol, caffeine, and nicotine), allowing students to collect data on 19 anatomical endpoints and generate significant amounts of data related to developmental toxicology and environmental health. This virtual laboratory offers students and instructors the choice of data sets that differ in the independent variables of chemical concentration and duration of postfertilization exposure. This enables students considerable flexibility in establishing their own experimental design to match the curriculum needs of each instructor.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"90 ","pages":"73-79"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140794206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tagging the tjp1a Gene in Zebrafish with Monomeric Red Fluorescent Protein Using Biotin Homology Arms. 用生物素同源臂为斑马鱼的 tjp1a 基因标记单体红色荧光蛋白。

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0096

Connor W Davison, Hamelynn Harzman, Jessie Nicholson, Seth M Entriken, Kierinn Mobley, Abigail G Krull, Manik Singhal, Caleb Skow, Nathan Matthews, Lindsey Kopp, Benjamin Gillette, Tyler J Weide, Jana R Hukvari, Sofia C P Stumpf, Olivia M Feldmann, M. McGrail, Renu Srivastava, J. Essner

{"title":"Tagging the tjp1a Gene in Zebrafish with Monomeric Red Fluorescent Protein Using Biotin Homology Arms.","authors":"Connor W Davison, Hamelynn Harzman, Jessie Nicholson, Seth M Entriken, Kierinn Mobley, Abigail G Krull, Manik Singhal, Caleb Skow, Nathan Matthews, Lindsey Kopp, Benjamin Gillette, Tyler J Weide, Jana R Hukvari, Sofia C P Stumpf, Olivia M Feldmann, M. McGrail, Renu Srivastava, J. Essner","doi":"10.1089/zeb.2023.0096","DOIUrl":"https://doi.org/10.1089/zeb.2023.0096","url":null,"abstract":"Tjp1a and other tight junction and adherens proteins play important roles in cell-cell adhesion, scaffolding, and forming seals between cells in epithelial and endothelial tissues. In this study, we labeled Tjp1a of zebrafish with the monomeric red fluorescent protein (mRFP) using CRISPR/Cas9-mediated targeted integration of biotin-labeled polymerase chain reaction (PCR) generated templates. Labeling Tjp1a with RFP allowed us to follow membrane and junctional dynamics of epithelial and endothelial cells throughout zebrafish embryo development. For targeted integration, we used short 35 bp homology arms on each side of the Cas9 genomic target site at the C-terminal of the coding sequence in tjp1a. Through PCR using 5' biotinylated primers containing the homology arms, we generated a double-stranded template for homology directed repair containing a flexible linker followed by RFP. Cas9 protein was complexed with the tjp1a gRNA before mixing with the repair template and microinjected into one-cell zebrafish embryos. We confirmed and recovered a precise integration allele at the desired site at the tjp1a C-terminus. Examination of fluorescence reveals RFP cell-cell junctional labeling using confocal imaging. We are currently using this stable tjp1a-mRFPis86 line to examine the behavior and interactions between cells during vascular formation in zebrafish.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"640 ","pages":"191-197"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140791119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fipronil Affects Craniofacial and Heart Development in Zebrafish Embryos (Danio rerio). 氟虫腈对斑马鱼胚胎（Danio rerio）颅面和心脏发育的影响

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0055

Kasey L Cooper, Zoe G Krut, Bennet D Franz, Benjamin S Walker, Alexander G Kramer, Jonathan R Morgan, Christopher S. Lassiter

引用次数: 0

Real-Time Observation of Germinal Vesicle Migration During Oocyte Meiotic Cell Division Using Ovarian Fluorescent Transgenic Zebrafish. 利用卵巢荧光转基因斑马鱼实时观察卵母细胞减数分裂过程中胚芽囊泡的迁移。

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0048

Eisei Tsutsumi, Toshinobu Tokumoto

引用次数: 0

Growing Project BioEYES: A Reflection on 20 Years of Developing and Replicating a K-12 Science Outreach Program. 发展项目 BioEYES：开发和复制 K-12 科学推广计划 20 年的反思。

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0059

Jamie R Shuda, Valerie G. Butler, Theresa M Nelson, Jaqueline M Davidson, Auset M Taylor, Steven A Farber

引用次数: 0

Zebrafish in Education: Tackling Big Problems with Little Fish. 教育中的斑马鱼：用小鱼解决大问题。

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2024.0141

M. Jackstadt, Lara Hutson, Jennifer O Liang, M. Pickart, Christopher Pierret, T. Franz-Odendaal

引用次数: 0

Development of an Undergraduate Cell Biology Laboratory to Assess Pigmentation and Cell Size in a Zebrafish Model of Uveal Melanoma. 开发本科生细胞生物学实验室，以评估斑马鱼葡萄膜黑色素瘤模型中的色素沉着和细胞大小。

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0095

Andrea M Henle

{"title":"Development of an Undergraduate Cell Biology Laboratory to Assess Pigmentation and Cell Size in a Zebrafish Model of Uveal Melanoma.","authors":"Andrea M Henle","doi":"10.1089/zeb.2023.0095","DOIUrl":"10.1089/zeb.2023.0095","url":null,"abstract":"This study outlines a 2-week laboratory module for an authentic cell biology undergraduate research experience that uses zebrafish (Danio rerio), a popular model organism for research. Previous research has indicated that course-based undergraduate research experiences such as this one increase student confidence, active learning, and retention. During this research experience, students investigate variations in pigmentation in the caudal fins of wild type (WT) and transgenic fish [Tg(mitfa:GNAQQ209L)]. The transgenic fish express a hyperactive Gα protein, GNAQQ209L, under the melanocyte-specific mitfa promoter, offering insights into uveal melanoma, a common eye cancer. Students specifically analyze the black pigmented cells, melanophores, within the caudal fin. We determined that the transgenic zebrafish have increased pigmentation in their caudal fins, but smaller melanophores. These results suggest there are more melanophores in the Tg(mitfa:GNAQQ209L) fish compared to the WT. Future undergraduate research could investigate these cellular differences. This research experience imparts microscopy and image analysis skills and instills the ability to grapple with large datasets, statistical tests, and data interpretation in alignment with biology education principles. Post-laboratory surveys reveal students attain confidence in the above skills and in handling animals, along with a deeper appreciation for model organism research and its relevance to cancer cell biology.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"21 2","pages":"137-143"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140873294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The InSciEdRS View: A User-Friendly and Accessible Microscope with Easy-to-Follow Companion Curricula. InSciEdRS View：用户界面友好、易于使用的显微镜，以及简单易学的配套课程。

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0093

Soaleha Shams, Sidney Olson, Michael P. Ekker, Adam Salmi, Stephen C Ekker, Christopher Pierret

{"title":"The InSciEdRS View: A User-Friendly and Accessible Microscope with Easy-to-Follow Companion Curricula.","authors":"Soaleha Shams, Sidney Olson, Michael P. Ekker, Adam Salmi, Stephen C Ekker, Christopher Pierret","doi":"10.1089/zeb.2023.0093","DOIUrl":"https://doi.org/10.1089/zeb.2023.0093","url":null,"abstract":"Microscopes are essential for research and education in science. Unlike computers and online learning tools, however, microscopes are not currently a fixed element in K-12 classrooms, due to steep cost, needless complexity, and often requiring a prohibitive level of staff training to effectively deploy. In a collaboration with Area 10 Labs, Integrated Science Education Outreach (InSciEd Out) developed a state-of-the-art alternative microscope, the InSciEdRS View, to reduce the financial barrier, prohibitive per-student cost, unnecessary complexity, and extensive staff training. Utilizing a 1080p camera and a lunchbox-style case, this Wi-Fi- and USB-connectable microscope comes with all necessary components for visualization of microscopic specimens (10 × -50 × magnification). While built to handle the rigors of classroom use, its imaging capability and battery-operation can make it flexible for a laboratory or fieldwork as well. We further highlight here K-12 curricula that we have developed using larval zebrafish to enable teachers, science outreach leaders, and parents to support active hands-on science observations. The InSciEdRS View microscope and the InSciEd Out curricula are readily scalable, translatable, and accessible for traditional and neurodiverse students and integrating these in various settings can be an efficient way to achieve better outcomes in science education.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"433 1","pages":"101-108"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140773156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lectins As Effective Tools in the Study of the Biliary Network and the Parenchymal Architecture of the Zebrafish (Danio rerio) Liver. 作为研究斑马鱼（Danio rerio）肝脏胆道网络和实质结构的有效工具的凝集素

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0073

Hayden Bhavsar, Zoe Robinson, Matthew Benson, Feven Getachew, J. González-Rosa, Pascal J Lafontant

引用次数: 0

Course on Breeding, Handling, and Experimental Procedures of Zebrafish (Danio rerio): Training, Refinement, Paradigm Shift, and Dissemination of Knowledge from Brazil to the World. 斑马鱼（Danio rerio）的繁殖、处理和实验程序课程：培训、改进、范式转变以及从巴西向世界传播知识。

Zebrafish Pub Date : 2024-04-01 DOI: 10.1089/zeb.2023.0065

Tony Silveira, Mateus Kütter, B. Nornberg, Marcela Meirelles, Dennis Silva, Andrea Idelette López, Andressa Lopes, Caroline Castro, Jenifer Moraes, Lucia Emanueli Schimith, Priscila de Leon, Luis Fernando Marins, Mariana Remião

{"title":"Course on Breeding, Handling, and Experimental Procedures of Zebrafish (Danio rerio): Training, Refinement, Paradigm Shift, and Dissemination of Knowledge from Brazil to the World.","authors":"Tony Silveira, Mateus Kütter, B. Nornberg, Marcela Meirelles, Dennis Silva, Andrea Idelette López, Andressa Lopes, Caroline Castro, Jenifer Moraes, Lucia Emanueli Schimith, Priscila de Leon, Luis Fernando Marins, Mariana Remião","doi":"10.1089/zeb.2023.0065","DOIUrl":"https://doi.org/10.1089/zeb.2023.0065","url":null,"abstract":"Currently, in Brazil, all researchers involved in animal experimentation must undergo training in laboratory animal science to stay updated on biology, methodology, ethics, and legal considerations related to the use of animals. The training program presented in this study not only aims to fulfill a legal obligation but also intends to train students and professionals to effectively care for their biomodels. It seeks to help them understand the importance of this care, both for the welfare of the animals and for the results of their projects. In total, 58 participants were present at the event (pre-event and full-time course). These participants consisted students and professionals from 11 institutions and 5 different countries. These numbers demonstrate the successful attainment of the desired capillarity in the scientific community and the posterior dissemination of knowledge. Through this course, it was possible to train the participants and raise their awareness about the importance of applying scientific knowledge in their daily practices to maintain the animals, ensuring the welfare of the models and refining the research. Finally, the program presented in this study, as well as the strategies adopted, can serve as a model for other institutions aiming to achieve similar results.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"52 37","pages":"155-161"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140796183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0