Zebrafish最新文献_第5页

Innovative Approaches in Developmental Biology: Integrating Zebrafish as a Model Organism in Education. 发展生物学的创新方法：将斑马鱼作为教育中的模式生物。

Zebrafish Pub Date : 2025-06-01 Epub Date: 2025-05-22 DOI: 10.1089/zeb.2024.0182

Magda Dubińska-Magiera, Ewa Stefanik, Marta Migocka-Patrzałek

引用次数: 0

Comprehensive 3D Imaging of Whole Zebrafish Using a Water-Based Clearing Reagent for Hard Tissues. 利用水基硬组织清除试剂对整个斑马鱼进行全面的三维成像。

IF 1.2

Zebrafish Pub Date : 2025-06-01 DOI: 10.1089/zeb.2025.0018

Kiyohito Taimatsu, John Prevedel, Daniel Castranova, Kanako Inoue, Amit Puthan, Brant M Weinstein

{"title":"Comprehensive 3D Imaging of Whole Zebrafish Using a Water-Based Clearing Reagent for Hard Tissues.","authors":"Kiyohito Taimatsu, John Prevedel, Daniel Castranova, Kanako Inoue, Amit Puthan, Brant M Weinstein","doi":"10.1089/zeb.2025.0018","DOIUrl":"10.1089/zeb.2025.0018","url":null,"abstract":"Zebrafish (Danio rerio) is a valuable model organism for studying developmental processes due to its external development and the optical clarity of its embryos and larvae. However, as development proceeds, zebrafish form increasingly opaque tissues that impede visualization of deep tissues and structures. Although tissue clearing methods have been used to facilitate imaging at these later stages, most of these methods have limited ability to clear dense tissues such as bone and cartilage, cause significant morphological distortion, and/or result in loss of fluorescent signal when used for imaging of fluorescent transgenes, dye-stained animals, or specimens generated using immunofluorescence or fluorescence in situ hybridization methods. Here, we report a novel imaging technique using a recently developed clearing reagent called LUCID that makes it possible to capture the complete cellular-resolution 3D structures of larval and juvenile zebrafish. We show that LUCID clears dense tissues such as pharyngeal cartilage in juvenile animals and even tooth bone in adults without causing either significant morphological distortion or significant loss of signal from transgene-driven fluorescent proteins, fluorescent nuclear DNA or actin staining dyes, or whole-mount in situ hybridization chain reaction fluorescence. Using this new approach, it is possible to perform complete high-resolution 3D imaging of whole fluorescently stained animals, even deep internal regions, providing a novel tool for elucidating the complex internal structures of developing zebrafish.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"22 3","pages":"65-75"},"PeriodicalIF":1.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12266943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144287705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Tricaine Methanesulfonate Concentrations for Flow Anesthesia in Zebrafish (Danio rerio). 斑马鱼流动麻醉中三卡因甲磺酸浓度的评价。

Zebrafish Pub Date : 2025-06-01 Epub Date: 2025-04-23 DOI: 10.1089/zeb.2025.0005

Alexander G Kramer, Logan R Bern, Lindsey T Ferguson, Michael B Palillo, Raphael A Malbrue, Mary E White, Genevieve C Kendall, Carmen B Arsuaga-Zorrilla, Logan A Fehrenbach

{"title":"Evaluation of Tricaine Methanesulfonate Concentrations for Flow Anesthesia in Zebrafish (Danio rerio).","authors":"Alexander G Kramer, Logan R Bern, Lindsey T Ferguson, Michael B Palillo, Raphael A Malbrue, Mary E White, Genevieve C Kendall, Carmen B Arsuaga-Zorrilla, Logan A Fehrenbach","doi":"10.1089/zeb.2025.0005","DOIUrl":"10.1089/zeb.2025.0005","url":null,"abstract":"Zebrafish (Danio rerio) are often anesthetized by immersion in buffered tricaine methanesulfonate (MS-222). Although commonly utilized, immersion anesthesia presents a shortcoming of lethal asphyxiation with increased duration of exposures. A newer technique that circumvents this issue, known as flow anesthesia, has been adapted from larger aquatic species to zebrafish. Flow anesthesia improves safety by delivering oxygen-rich water along with the anesthetic across gill epithelium and allowing fish to be manipulated outside of water. Information on the construction of flow anesthesia apparatuses and parameters are sparse. The goal of this study was to create a flow anesthesia apparatus with materials commonly found within a research facility and to evaluate variable concentrations of MS-222 for anesthesia in zebrafish. Depth of anesthesia was monitored by quantifying respiratory rate and recording responses to physical stimulation. All concentrations of MS-222 evaluated (30-100 ppm) were successful at maintaining surgical anesthesia for up to 30 min. The anesthetic events were demonstrated to be safe, with an observed 97% survival rate. This work demonstrates refinements in zebrafish anesthesia and encourages future studies to evaluate MS-222 flow anesthesia for longer duration and evaluation of other commercially available anesthetics for efficacy in a flow anesthesia setup.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"99-106"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding Rigor Mortis Impacts on Zebrafish Gamete Viability. 了解僵死对斑马鱼配子活力的影响。

Zebrafish Pub Date : 2025-04-01 Epub Date: 2025-02-27 DOI: 10.1089/zeb.2024.0177

Renata Villar Dantas, Lis Santos Marques, Thaiza Rodrigues Freitas, Nathalia Dos Santos Teixeira, Rômulo Batista Rodrigues, Jhony Lisbôa Benato, Raquel Santos Dos Santos, Diógenes Henrique Siqueira Silva, Danilo Pedro Streit

{"title":"Understanding Rigor Mortis Impacts on Zebrafish Gamete Viability.","authors":"Renata Villar Dantas, Lis Santos Marques, Thaiza Rodrigues Freitas, Nathalia Dos Santos Teixeira, Rômulo Batista Rodrigues, Jhony Lisbôa Benato, Raquel Santos Dos Santos, Diógenes Henrique Siqueira Silva, Danilo Pedro Streit","doi":"10.1089/zeb.2024.0177","DOIUrl":"10.1089/zeb.2024.0177","url":null,"abstract":"This study aimed to evaluate the viability of gametes in zebrafish (Danio rerio), at different rigor mortis stages. Viability assessments were conducted on oocytes at various developmental stages using LIVE/DEAD and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. For sperm evaluation, both kinetic (computer-assisted sperm analysis) and morphological assessments (Rose Bengal staining) were performed. Results demonstrated that rigor mortis progression significantly impacted oocyte viability during post-rigor stages, with the following viability rates: pre-rigor (70.43 ± 12.31%), fresh/control (46.43 ± 12.54%), post-rigor (27.62 ± 22.29%), and rigor mortis (comparable to fresh/control). Conversely, sperm kinetics exhibited nuanced responses to the rigor mortis stages, with specific parameters showing sensitivity, whereas the others remained relatively stable. Sperm motility was higher in the fresh/control (63.23 ± 19.03%) and pre-rigor (58.96 ± 14.38%) compared to the post-rigor group (3.34 ± 4.65%). This study highlights the significance of the pre-rigor for successful gamete collection and preservation. These findings provide valuable insights for conservation efforts and optimization of genetic resource management for endangered fish species. This study aimed to develop effective assistive reproductive techniques by elucidating the interplay between rigor mortis and gamete quality, contributing to the broader goals of species conservation and maintenance of genetic diversity in fish populations.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"31-41"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143525759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VMS-Fish: A Comprehensive Application for Zebrafish Husbandry Management. VMS-Fish：斑马鱼养殖管理的综合应用。

IF 1.2

Zebrafish Pub Date : 2025-04-01 Epub Date: 2025-04-08 DOI: 10.1089/zeb.2024.0183

Vishnu Muraleedharan Saraswathy, Mayssa H Mokalled

引用次数: 0

An Improved, High-Yield Method for Isolating Nuclei from Individual Zebrafish Embryos for Single-Nucleus RNA Sequencing. 一种改进的、高产的从单个斑马鱼胚胎中分离细胞核进行单核RNA测序的方法。

IF 1.2

Zebrafish Pub Date : 2025-04-01 Epub Date: 2025-03-05 DOI: 10.1089/zeb.2024.0175

Clifford Rostomily, Heidi Lee, Amy Tresenrider, Riza Daza, Andrew Mullen, Jay Shendure, David Kimelman, Cole Trapnell

引用次数: 0

Small Fishes, Big Issues: Species Delimitation in Hemigrammus Marginatus, Gill, 1958 (Acestrorhamphidae: Pristellinae) from Brazilian Coastal Basins Based on Integrative Genetics. 小鱼类，大问题：基于整合遗传学的巴西沿海流域 Hemigrammus Marginatus, Gill, 1958 (Acestrorhamphidae: Pristellinae) 鱼种划分。

Zebrafish Pub Date : 2025-04-01 Epub Date: 2025-02-14 DOI: 10.1089/zeb.2024.0174

Mauricio B Fernandes, Jamille de Araújo Bitencourt, André Teixeira da Silva, Marcelo Ricardo Vicari, Matheus Azambuja, Paulo Roberto Antunes de Mello Affonso

{"title":"Small Fishes, Big Issues: Species Delimitation in Hemigrammus Marginatus, Gill, 1958 (Acestrorhamphidae: Pristellinae) from Brazilian Coastal Basins Based on Integrative Genetics.","authors":"Mauricio B Fernandes, Jamille de Araújo Bitencourt, André Teixeira da Silva, Marcelo Ricardo Vicari, Matheus Azambuja, Paulo Roberto Antunes de Mello Affonso","doi":"10.1089/zeb.2024.0174","DOIUrl":"10.1089/zeb.2024.0174","url":null,"abstract":"The small characins represent a systematic puzzle in the Neotropical ichthyofauna as a result of independent miniaturization processes, adaptive convergence and lack of diagnostic characters for several genera. In order to diminish the taxonomic uncertainties and the evolutionary pathways in Hemigrammus, we carried out an integrative genetic analysis in the putatively widespread Hemigrammus marginatus Ellis, 1958 by combining cytogenetic and molecular data based on the mitochondrial Cytochrome C Oxidase subunit I (COI). Specimens of H. marginatus from the type locality in Itapicuru River basin and other two populations from coastal rivers in northeastern Brazil were analyzed and compared with the available data from other regions in South America. Conspicuous macro and microkaryotypic differences were detected between the samples from northeastern and southern Brazil (Upper Paraná River basin). Likewise, the DNA barcoding and species delimitation analyses recovered distinct Molecular Operational Taxonomical Units within H. marginatus. Therefore, the population from the type locality should be referred to as H. marginatus stricto sensu, representing a restricted characin taxon from coastal drainages (including the São Francisco River basin) along northeastern Brazil, while other populations of this small characin fish need to be taxonomically revised and managed as unique lineages.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"46-58"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143417133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Food Delivery Method for Learning Studies Detects Significant Differences in Food Preference in Zebrafish. 一种用于学习研究的新型食物传递方法检测斑马鱼食物偏好的显着差异。

Zebrafish Pub Date : 2025-02-01 Epub Date: 2025-02-06 DOI: 10.1089/zeb.2024.0176

Amira Abozaid, Melissa Scaramuzzo, Hemali Patel, Robert Gerlai

{"title":"A Novel Food Delivery Method for Learning Studies Detects Significant Differences in Food Preference in Zebrafish.","authors":"Amira Abozaid, Melissa Scaramuzzo, Hemali Patel, Robert Gerlai","doi":"10.1089/zeb.2024.0176","DOIUrl":"10.1089/zeb.2024.0176","url":null,"abstract":"Despite decades of research with laboratory rodents, the mechanisms underlying learning and memory, and their impairment, are still not fully understood. The zebrafish is a newcomer in this research area, but it has shown great promise. Food is often employed as a reinforcer in learning tasks with rodents. However, for zebrafish, food has been a problematic reinforcer. Controlling timing and localization of its delivery is difficult. What food types zebrafish prefer is also rarely studied? Here, we describe a novel food delivery hardware and procedure. The apparatus is simple, cheap to manufacture, and easy to employ. Using this new method, we compare how zebrafish respond to three food types, artemia nauplii, crushed tropical fish flakes, and small zebrafish pellets. In binary choice tasks, we show that zebrafish spend significantly more time near the artemia delivery cylinder, swim closer to, and visit this cylinder more frequently compared to food cylinders delivering flakes or pellets, while responses to these latter two cylinders do not differ from each other. We conclude that the newly developed method allows the quantification of food preference in zebrafish, and that it will lead to the identification of highly rewarding food types for learning studies in this species.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"20-29"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgment of Reviewers 2024. 审稿人致谢

Zebrafish Pub Date : 2025-02-01 DOI: 10.1089/zeb.2024.58862.revack

引用次数: 0

An Automated Low-Cost Solution for Creating a Multiple-Step Thermal Gradient to Record Daily Fish Thermoregulatory Behavior. 一种自动化的低成本解决方案，用于创建多步热梯度来记录鱼类的日常温度调节行为。

Zebrafish Pub Date : 2025-02-01 Epub Date: 2025-02-06 DOI: 10.1089/zeb.2024.0153

Francesca Conti, Gonzalo de Alba, Jose F López-Olmeda, Luisa M Vera, Gregory Lucon-Xiccato, Elena Mainardi, Silvia Cesari, Michele Bottarelli, Cristiano Bertolucci, Francisco J Sánchez-Vázquez, Elia Gatto

引用次数: 0