Ana Paula Apolinário da Silva, João Xavier da Silva Neto, José Ytalo Gomes da Silva, Ana Lorena Pereira Bezerra, Bruno Bezerra da Silva, Maria Izabel Florindo Guedes
{"title":"Modified Procedure for Simple and Rapid Collection of Zebrafish Fluid Samples for Biochemical Analyses.","authors":"Ana Paula Apolinário da Silva, João Xavier da Silva Neto, José Ytalo Gomes da Silva, Ana Lorena Pereira Bezerra, Bruno Bezerra da Silva, Maria Izabel Florindo Guedes","doi":"10.1089/zeb.2023.0101","DOIUrl":null,"url":null,"abstract":"<p><p>This study addresses the challenge of collecting blood samples from zebrafish for biochemical analysis. Traditional methods are cumbersome due to low blood flow and rapid coagulation. Based on a previously published technique, we simplified the process by applying an anticoagulant solution directly to the incision site. The modified protocol involves immersing the fish in an ice bath, making a cross-sectional incision, and immediately applying anticoagulant solution. Centrifugation of the specimens provides a streamlined and efficient approach to zebrafish fluid sample collection, compatible with classic biochemical marker analyses.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"259-264"},"PeriodicalIF":0.0000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zebrafish","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/zeb.2023.0101","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/20 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
This study addresses the challenge of collecting blood samples from zebrafish for biochemical analysis. Traditional methods are cumbersome due to low blood flow and rapid coagulation. Based on a previously published technique, we simplified the process by applying an anticoagulant solution directly to the incision site. The modified protocol involves immersing the fish in an ice bath, making a cross-sectional incision, and immediately applying anticoagulant solution. Centrifugation of the specimens provides a streamlined and efficient approach to zebrafish fluid sample collection, compatible with classic biochemical marker analyses.
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