Zebrafish最新文献

{"title":"Feeding Larval Zebrafish: Small Predators, Big Choices.","authors":"Tierney B Daw, Savanna C Sheridan, Cameron M Williams, Cadell D Perry, Mary D Kinkel","doi":"10.1177/15458547261493906","DOIUrl":"https://doi.org/10.1177/15458547261493906","url":null,"abstract":"<p><p>Zebrafish husbandry regimens often use live rotifers or paramecia during the first 2 weeks of larval feeding. The rationale is that the small larval gape requires suitably small prey. As larvae grow, they are transitioned to feeding on larger prey in the form of brine shrimp (<i>Artemia</i>). Typically, the <i>Artemia</i> used are the San Francisco Bay (SFB) strain. This strain is reported to be smaller than the Great Salt Lake (GSL) strain and is presumably easier for larvae to capture. Here, we tested the ability of zebrafish larvae to capture either strain. Daily, from 5 to 15 days post-fertilization (dpf), intestinal contents were imaged through the body wall of live larvae following shrimp feeding. Larvae showed increased capture success day by day, and by 9 dpf most larvae captured the SFB strain. By contrast, few larvae captured the GSL strain throughout the study. Morphometric tests comparing the strains showed no differences in length or width for newly hatched shrimp. However, after 48 h of culturing, the GSL strain was significantly larger than the SFB strain. For husbandry regimens that omit rotifers or paramecia and introduce shrimp from the first feeding, these results highlight the importance of using newly hatched SFB <i>Artemia</i>.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"15458547261493906"},"PeriodicalIF":1.2,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149129690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Variant Diet on Growth and Boldness in Juvenile Trinidadian Guppies (<i>Poecilia Reticulata</i>).","authors":"Theresa Muraca, Kiyoko M Gotanda","doi":"10.1177/15458547261491044","DOIUrl":"https://doi.org/10.1177/15458547261491044","url":null,"abstract":"<p><p>Diet is an integral component of the welfare of captive animals. Previous studies have shown that diet can influence species growth and behavior. However, whether a variant diet provides enhanced benefits for captive animals, like the Trinidadian guppy, is not known. We fed aquarium juvenile guppies three different diets for 4 weeks and assessed growth rate and changes in boldness behavior between the start and end of the experiment. Diet type did not have an effect on either growth or boldness. We did find that guppies fed a variant diet exhibited more boldness at the end of the experiment compared with the start. Our results suggest that the type of diet fed to juvenile guppies can be used interchangeably with minimal effects on growth or boldness. Diet is an essential aspect of animal welfare, and further research allows for effective captive practices to improve and maintain the survival of aquatic species.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"15458547261491044"},"PeriodicalIF":1.2,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Method for Measuring Intraocular Pressure in Wild-Type Zebrafish Using Rebound Tonometry: Characterization, Sex Differences, and Long-Term Repeatability.","authors":"Hsin-Lin Cheng, Pei-Hsuan Chou, Yung-Chang Yen, Jiann-Jou Yang","doi":"10.1177/15458547261477301","DOIUrl":"https://doi.org/10.1177/15458547261477301","url":null,"abstract":"<p><p>Intraocular pressure (IOP) monitoring is a critical aspect of diagnosis, and management of ocular diseases. Therefore, a tractable vertebrate model capable of reliable IOP characterization is highly desirable. The present study aims to perform a preliminary investigation of a handheld rebound tonometer to stably measure IOP and further characterize baseline IOP values in the zebrafish model. The IOP in both eyes (oculus dexter, OD, and oculus sinister, OS) of wild-type zebrafish was measured using the iCare PRO rebound tonometer at the same time points on testing days for 18 months. The long-term IOP values of male zebrafish (3-21 months) were range from 21.96 ± 0.99 to 23.39 ± 0.32 mm Hg (OD) and 22.10 ± 0.93 to 23.38 ± 0.26 mm Hg (OS), while insignificant difference was noted among testing days (<i>p</i> > 0.05). Similarly, insignificant difference was observed in female zebrafish with IOP values range from 22.10 ± 1.12 to 23.19 ± 0.72 mm Hg (OD) and 22.30 ± 1.00 to 23.15 ± 0.36 mm Hg (OS) (<i>p</i> > 0.05). A comprehensive evaluation of IOP profiles confirmed no significant differences based on gender, time point, and between eyes. These findings indicated that our iCare tonometer-based experimental methods provide high measurement and time-point consistency, confirming their long-term reproducibility and reliability in measuring IOP in the zebrafish.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"15458547261477301"},"PeriodicalIF":1.2,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148709290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UCI BioEYEs: A Graduate-Student-Led Model for Sustainable, Inclusive Science Outreach.","authors":"Michael J Parsons, Raquel L Bowman, Kathleen N Leon Parada, David Bojorquez, Chanel Lewis, Brooke Del Padre, Travis J Wiles, Star W Lee","doi":"10.1177/15458547261476961","DOIUrl":"https://doi.org/10.1177/15458547261476961","url":null,"abstract":"<p><p>Zebrafish are a powerful, accessible vertebrate model for teaching core biology concepts. BioEYES is a well-established K-12 outreach program that uses inquiry-based classroom investigations to leverage these strengths. However, its traditional model relies on dedicated personnel and substantial infrastructure, limiting sustainability at individual institutions. Here, we describe UCI BioEYEs, a graduate student-led adaptation that maintains BioEYES' educational strengths while reducing logistical and financial barriers. In partnership with middle school science teachers at a dual-language (English-Spanish) campus, UCI BioEYEs delivers week-long modules built around live zebrafish embryos and larvae. Graduate students lead hands-on investigations in cell biology, development, environmental effects on embryogenesis, and Mendelian inheritance. Activities use standard classroom equipment (e.g., school-grade stereoscopes, smartphone-based imaging), enabling repeated use without specialized infrastructure. Over four years, UCI BioEYEs reached over 1,100 middle school students. Teachers cited curriculum integration, sustained engagement with live vertebrate models, graduate student role models, and inclusive bilingual design as key to long-term adoption. Graduate instructors reported major gains in teaching confidence and science communication skills. Thus, a graduate student-led structure sustains zebrafish-based outreach benefiting K-12 learners while supporting graduate training. UCI BioEYEs offers a transferable framework for scalable, inclusive science education. All curricular materials are provided here to support adoption by other institutions.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"15458547261476961"},"PeriodicalIF":1.2,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148702761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Precision Pneumatic Induction of Traumatic Brain Injury in Larval Zebrafish.","authors":"Kunrong Wang, Ping Zhang, Yijie Geng","doi":"10.1177/15458547261469710","DOIUrl":"10.1177/15458547261469710","url":null,"abstract":"<p><p>Zebrafish are an emerging vertebrate model for traumatic brain injury (TBI) research, but current larval injury paradigms, especially weight-drop methods, can be difficult to standardize. Here, we describe the Zebrafish Pneumatic Injury Device (ZePID), a compact pneumatic piston system that delivers controlled, reproducible pressure pulses to induce TBI in larval zebrafish. ZePID showed a strong linear relationship between input and delivered pressure and reduced variability relative to weight-drop approaches. In 6-days postfertilization larvae, a 150 psi injury increased maximum swimming speed and total distance traveled, consistent with seizure-like hyperactivity after TBI.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"132-136"},"PeriodicalIF":1.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148521629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Auxin Inducible Protein Degradation in Zebrafish.","authors":"Robert D Morabito, Benjamin L Martin","doi":"10.1177/15458547261456251","DOIUrl":"10.1177/15458547261456251","url":null,"abstract":"","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"125-126"},"PeriodicalIF":1.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148059296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Essential Oil of <i>Varronia curassavica</i> (Cordiaceae) as an Anesthetic and Sedative for Zebrafish.","authors":"Ana Júlia Redante, Larissa Kochenborger Behm, Carlos Herminio Magalhães Fortes, Ana Paula Giolo Franz, Emilly Baldissareli, Amanda Correa Santos, João Antônio Duarte Lampugnani, Wagner Antonio Tamagno, Leonardo José Gil Barcellos","doi":"10.1177/15458547261461539","DOIUrl":"10.1177/15458547261461539","url":null,"abstract":"<p><p>In many research studies, the handling and procedures performed can generate stress and require the use of anesthesia or sedation. In this sense, plant-based extracts named essential oils have emerged as potentially effective and environmentally safe anesthetic/sedative options. Here, we sought to evaluate the essential oil of <i>Varronia curassavica</i> (VCEO) as an anesthetic/sedative ingredient. Here, the anesthetic and sedative capabilities of the VCEO using adult zebrafish (<i>Danio rerio</i>). The anesthetic induction was performed under four different concentrations (100, 200, 400, and 600 mg L^-1), followed by evaluation of recovery time; furthermore, a 3 h long exposure was performed for sedative testing, where fish were exposed for 3 h at three different concentrations (50, 80, and 100 mg L^-1) under a superpopulation condition, such as those faced during transport. To assess stress, we collected whole-body cortisol from each individual after each exposure. The concentrations of 400 and 600 mg L^-1 induced anesthesia (S4) but promoted excessive recovery times; on the contrary, they reduced the cortisol levels of the fish compared with the positive and negative controls. In the sedative testing (3 h long exposure), the animals exposed to 50 mg L^-1 remained in S2 stage from the first 10 min, and those exposed to 80 mg L^-1 after 20 min. The 100 mg L^-1 induced stage S3a. There were no significant changes in cortisol levels observed at these concentrations. Limitations include measurement of whole-body cortisol at a single endpoint and the consistently slower induction and recovery with VCEO compared with a reference anesthetic MS-222, a property that constrains its practical use and warrants further dose-response, time-course, and recovery-optimization studies. Therefore, VCEO appears to be a promising anesthetic and sedative alternative, effective and plant-based, without increasing the physiological stress of the animals.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"116-124"},"PeriodicalIF":1.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148304622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic Population Breeding: A Structured Colony Management Strategy to Improve Reproductive Performance and Early Survival in <i>Nothobranchius furzeri</i>.","authors":"Uta Naumann, Julia Hammerer, Clemens Peters, Simone Gruner, Martin Neumann, Beate Hoppe","doi":"10.1177/15458547261460388","DOIUrl":"10.1177/15458547261460388","url":null,"abstract":"<p><p>The African turquoise killifish, <i>Nothobranchius furzeri</i>, has emerged as an important vertebrate model for aging research due to its naturally short lifespan and hallmarks of aging. However, these characteristics create challenges for colony management, such as high breeder turnover, rapid generational change, and the risk of inbreeding or unintended selection in closed laboratory populations. Since new individuals cannot be introduced from the wild or purchased from commercial laboratory animal breeders, maintaining genetically stable laboratory stocks requires carefully controlled breeding strategies. Here, we describe an innovative colony management approach termed dynamic population breeding (DPB) and evaluated its performance in two commonly used laboratory lines with different lifespans, GRZ-D and MZCS-08/122. DPB integrates several key principles: the maintenance of overlapping breeding age cohorts, harem-based breeding groups, continuous monitoring of clutch quality and quantity, and the controlled use of embryonic diapause for flexible embryo storage and synchronized hatching. Embryos from multiple breeding groups were pooled, stored in diapause stage II, and hatched at defined time points to generate new cohorts while avoiding sibling-only populations. Using this approach, we systematically analyzed reproductive performance, embryo quality, hatching success, and survival in laboratory populations. Fertilization rates and clutch sizes remained within stable ranges across the reproductive period, although an age-dependent decline in fertilization efficiency was observed in both lines. Quality control at the clutch level allowed early identification and exclusion of low-performing breeding groups. Importantly, implementation of DPB reduced variability between cohorts and improved early-life survival of offspring, particularly during the first weeks after hatching. Overall, DPB provides a practical framework for maintaining stable and robust killifish colonies while minimizing unintended selection and inbreeding. By integrating diapause biology with structured breeding management, this strategy enhances reproducibility and sustainability of <i>N. furzeri</i> populations used in aging research.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"105-115"},"PeriodicalIF":1.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148240957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Fabrication of Highly Customizable Test Chambers for Behavioral Experiments with Small Aquatic Model Organisms.","authors":"Hy Do, Florian Kreuder, Xuhui Han, Michael Didham, Savita Kumari, Oskar Wasielewski, Jan Kaslin, Paul A Ramsland, David Mawdsley, Donald Wlodkowic","doi":"10.1177/15458547261468987","DOIUrl":"10.1177/15458547261468987","url":null,"abstract":"<p><p>Behavioral biotests with larval zebrafish and small aquatic invertebrates are predominantly conducted using digital video recording combined with specialized animal tracking software to extract quantitative locomotor and behavioral endpoints. Such assays commonly rely on commercially available polystyrene multi-well test plates, which, despite their convenience, present several limitations: fixed well geometry, meniscus-induced shadow artifacts at well edges, absence of customization options, and suboptimal optical transmittance for orthogonal sidelight illumination with infrared light. Here, we describe a rapid and low-cost technique for fabricating highly customizable test chambers for behavioral experiments with small aquatic model organisms. The method employs infrared laser cutting of biocompatible poly(methyl methacrylate) thermoplastic followed by thermal bonding under controlled compression in a standard laboratory oven. The speed and low cost of fabrication enable the production of large numbers of test plates with considerable flexibility in chamber geometry, including complex miniaturized maze configurations. As a proof of concept, we demonstrate how test chamber geometry influences both baseline locomotor activity and stimulated sensory-motor responses in larval zebrafish. Relatively little is currently understood about how larval zebrafish-with their tractable and well-characterized nervous systems-perceive and respond to spatial environmental features or how chamber geometry modulates common behavioral responses. The fabrication technique described here provides researchers with a flexible and accessible tool for developing novel behavioral biotests with broad applications across ecology, ecotoxicology, and experimental neurobiology.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"127-131"},"PeriodicalIF":1.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions Between Feeding Protocols, Larval Density, and Methylene Blue During Zebrafish Larval Development.","authors":"Ahmed Almaghasilah, Daniela Chavez de Paz Solis, Emily Frazer, Grace Hodgkin, Shira Hollinger-Levitsky, Marcus Russano, Clarissa A Henry","doi":"10.1177/15458547261434500","DOIUrl":"10.1177/15458547261434500","url":null,"abstract":"<p><p>Conducting longitudinal experiments on zebrafish disease models has the potential to deepen understanding of disease progression. Longitudinal experiments require rearing of zebrafish embryos/larvae in the laboratory for extended time periods to enable use of laboratory equipment for serial observation. We observed that in our laboratory, wild-type larvae did not survive past 14 days. As we were unable to identify a study that compared different factors that could affect survival of larvae raised in the laboratory, we aimed to define the most relevant parameters that impact larval survival and growth. We found that prolonged access to food had the greatest beneficial effect on survival, regardless of rearing density or rearing media. However, rearing density did impact the activity of 2.5-week-old larvae. Methylene blue is a frequent additive to zebrafish embryo-rearing medium. Methylene blue can impact cellular health, and methylene blue mitigated the decrease in motility of densely grown larvae. Methylene blue also promoted growth: larvae raised in methylene blue were significantly larger at 1 month than their counterparts raised without methylene blue. These data highlight factors that promote survival and growth of larval zebrafish raised in the laboratory for longitudinal studies.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"41-45"},"PeriodicalIF":1.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147694644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}