IF 1.2

Zebrafish Pub Date : 2026-05-01 DOI: 10.1177/15458547261448182

Salome Brodd, Friedrich G Kapp

引用次数: 0

Interactions Between Feeding Protocols, Larval Density, and Methylene Blue During Zebrafish Larval Development. 斑马鱼幼体发育过程中摄食方案、幼体密度和亚甲基蓝的相互作用。

IF 1.2

Zebrafish Pub Date : 2026-04-15 DOI: 10.1177/15458547261434500

Ahmed Almaghasilah, Daniela Chavez de Paz Solis, Emily Frazer, Grace Hodgkin, Shira Hollinger-Levitsky, Marcus Russano, Clarissa A Henry

{"title":"Interactions Between Feeding Protocols, Larval Density, and Methylene Blue During Zebrafish Larval Development.","authors":"Ahmed Almaghasilah, Daniela Chavez de Paz Solis, Emily Frazer, Grace Hodgkin, Shira Hollinger-Levitsky, Marcus Russano, Clarissa A Henry","doi":"10.1177/15458547261434500","DOIUrl":"https://doi.org/10.1177/15458547261434500","url":null,"abstract":"Conducting longitudinal experiments on zebrafish disease models has the potential to deepen understanding of disease progression. Longitudinal experiments require rearing of zebrafish embryos/larvae in the laboratory for extended time periods to enable use of laboratory equipment for serial observation. We observed that in our laboratory, wild-type larvae did not survive past 14 days. As we were unable to identify a study that compared different factors that could affect survival of larvae raised in the laboratory, we aimed to define the most relevant parameters that impact larval survival and growth. We found that prolonged access to food had the greatest beneficial effect on survival, regardless of rearing density or rearing media. However, rearing density did impact the activity of 2.5-week-old larvae. Methylene blue is a frequent additive to zebrafish embryo-rearing medium. Methylene blue can impact cellular health, and methylene blue mitigated the decrease in motility of densely grown larvae. Methylene blue also promoted growth: larvae raised in methylene blue were significantly larger at 1 month than their counterparts raised without methylene blue. These data highlight factors that promote survival and growth of larval zebrafish raised in the laboratory for longitudinal studies.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"15458547261434500"},"PeriodicalIF":1.2,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147694644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Equilibration Time to Enhance Post-Thaw Viability of Cryopreserved Zebrafish (Danio rerio) Ovarian Fragments. 优化平衡时间以提高冷冻保存斑马鱼卵巢碎片解冻后的活力。

IF 1.2

Zebrafish Pub Date : 2026-04-14 DOI: 10.1177/15458547261441744

Maya L Wade, Matthew K Litvak

{"title":"Optimizing Equilibration Time to Enhance Post-Thaw Viability of Cryopreserved Zebrafish (Danio rerio) Ovarian Fragments.","authors":"Maya L Wade, Matthew K Litvak","doi":"10.1177/15458547261441744","DOIUrl":"https://doi.org/10.1177/15458547261441744","url":null,"abstract":"Cryopreservation enables the long-term storage of viable biological material at ultra-low temperatures and forms the foundation for germplasm cryobanks that maintain valuable genetic lines of model organisms such as zebrafish (Danio rerio). However, reliable and reproducible cryopreservation protocols for fish germline stem cells remain difficult to develop, partly because key steps such as equilibration are often overlooked or assigned arbitrarily. Here, we optimized equilibration time for cryopreservation of zebrafish ovarian tissue. Ovarian fragments were equilibrated in 2 M methanol + 0.1 M glucose + 10% egg yolk for varying durations (15-120 min) before controlled slow cooling and storage in liquid nitrogen. Post-thaw viability was assessed using a Trypan Blue exclusion assay. A 60-min equilibration yielded the highest viability of ovarian cells in Experiment 1 (55.7 ± 1.7%), whereas a 30-min equilibration yielded the highest viability in Experiment 2 (75.9 ± 2.4%), but was not significantly different from the 60-min equilibration in Experiment 2 (75.6 ± 2.0%) (p = 0.998). Equilibration alone accounted for a 48.7% increase in post-thaw viability relative to controls. The framework presented here provides a reproducible method for determining species-specific equilibration optima and supports the development of effective germplasm cryobanks for both model and endangered fish species.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"15458547261441744"},"PeriodicalIF":1.2,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147694696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micro- and Nanoplastics Exposure and Effects on Embryonic Development and Larval Behavior of Zebrafish. 微纳米塑料暴露及其对斑马鱼胚胎发育和幼虫行为的影响。

IF 1.2

Zebrafish Pub Date : 2026-04-08 DOI: 10.1177/15458547261441746

Kaila Savage, Tiara Harris, Luke Itomura, Corina Liang, Keala Mak, Zachary Oglesby, Vincent Ooi, Gemma Quiroz, Kelley M Withy, Chris Pierret, Soaleha Shams

{"title":"Micro- and Nanoplastics Exposure and Effects on Embryonic Development and Larval Behavior of Zebrafish.","authors":"Kaila Savage, Tiara Harris, Luke Itomura, Corina Liang, Keala Mak, Zachary Oglesby, Vincent Ooi, Gemma Quiroz, Kelley M Withy, Chris Pierret, Soaleha Shams","doi":"10.1177/15458547261441746","DOIUrl":"https://doi.org/10.1177/15458547261441746","url":null,"abstract":"Hawai'i and other island communities around the globe are experiencing high exposures to micro- and nanoplastics (MNPs). Widespread use of various types of plastics and the action of wave physics coupled with abundant silica creates a constant production of MNPs. The community health effects of exposure to MNPs are not yet fully understood. The aquatic vertebrate model zebrafish, Danio rerio, was utilized to evaluate the developmental and behavioral effects of nanoplastic (NP) exposure as well as visual confirmation of plastic accumulation after embryonic and larval exposure. Eye and yolk size, heart rate, angle of development, and early larval locomotion behavior were quantified after early NP exposure lasting 3 or 5 days. In this work, a digital laboratory environment is used as the context for research trainees spanning high school to graduate and medical school in Hawai'i to explore health outcomes of developmental NP exposures. Presence of MNPs in the early developmental environment led to changes in growth and behavior of exposed fish. Data analysis showed significant effects of exposures on changes in the rate of development and yolk consumption on day 2 and reduced body length and increased locomotion on day 6. Nanoplastic exposure also affected overall body length, but this effect was not significant. These data support the potential dangers of MNP exposure and demonstrate their persistent detrimental effects on development and behavior in young zebrafish. Accordingly, our work contributes toward shifting focus toward understanding longer impacts on health, growth, and behaviors and potentially the reduction of harm in developing vertebrates.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"15458547261441746"},"PeriodicalIF":1.2,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147641133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ewsr1a Regulates the Development of the Axial Skeleton in Zebrafish. Ewsr1a调控斑马鱼轴向骨架的发育

IF 1.2

Zebrafish Pub Date : 2026-04-05 DOI: 10.1177/15458547261438422

Chris Merkes, Hyewon Park, Mizuki Azuma

{"title":"Ewsr1a Regulates the Development of the Axial Skeleton in Zebrafish.","authors":"Chris Merkes, Hyewon Park, Mizuki Azuma","doi":"10.1177/15458547261438422","DOIUrl":"https://doi.org/10.1177/15458547261438422","url":null,"abstract":"The EWSR1 (EWS RNA binding protein 1) was originally discovered in Ewing sarcoma, a pediatric bone cancer. In a previous study, we identified zebrafish ewsr1a, the homologue of human EWSR1. Using a zebrafish mutant for ewsr1a, we demonstrated that Ewsr1a promotes differentiation of chondrogenesis in Meckel's cartilage, a component of the facial skeletal elements. Furthermore, we demonstrated that Ewsr1a interacts with the transcription factor SRY-BOX transcription factor 9 (SOX9) and modulates the transcription of its target genes. In this study, the role of Ewsr1a was further investigated in axial skeletogenesis. The maternal zygotic (MZ) ewsr1a/ewsr1a zebrafish display curved spines derived from irregularly spaced intervertebral discs (IVDs) and aberrant differentiation of nucleus pulposus cells. Using an in vitro cell culture system, we further show that the N-terminus of Ewsr1a is required for the interaction with Sox9. In zebrafish, the Ewsr1a also interacts with a Sox9 target gene, specifically the first intron of col2a1a. Zebrafish with the MZ ewsr1a/ewsr1a genotype display an increased level of collagen type II protein in the notochord starting at 36 h post fertilization. We propose that Ewsr1a contributes to IVD formation by regulating the expression of col2a1a.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"15458547261438422"},"PeriodicalIF":1.2,"publicationDate":"2026-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147625153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Baby Catchers: A Novel Tank Design to Dramatically Improve Fry Recovery of Live-bearing Fishes. 婴儿捕手：一种新颖的水箱设计，以显着提高活鱼的鱼苗恢复。

IF 1.2

Zebrafish Pub Date : 2026-02-01 Epub Date: 2026-02-11 DOI: 10.1177/15458547261424193

Abi M Zuber, Helen Stec, Maximiliano Zuluaga-Forero, Ehren J Bentz, Meridia Jane Bryant, Matthew D Taves, Joli Vega, Lei Cao, Lynn Capani-Czebiniak, Brian Brown, Benjamin A Sandkam

{"title":"Baby Catchers: A Novel Tank Design to Dramatically Improve Fry Recovery of Live-bearing Fishes.","authors":"Abi M Zuber, Helen Stec, Maximiliano Zuluaga-Forero, Ehren J Bentz, Meridia Jane Bryant, Matthew D Taves, Joli Vega, Lei Cao, Lynn Capani-Czebiniak, Brian Brown, Benjamin A Sandkam","doi":"10.1177/15458547261424193","DOIUrl":"10.1177/15458547261424193","url":null,"abstract":"Live-bearing fishes (Poeciliidae) are increasingly used as laboratory models, but their utility is hampered by high rates of filial cannibalism, which reduces fry recovery and increases husbandry demands. Traditional strategies, such as mesh tank inserts (TIs), rely on fry actively seeking refuge and are often ineffective. Here, we present a novel tank design called Baby Catchers (BCs) that use water flow to passively and automatically separate newborn fry from their mothers, fitting seamlessly into standard recirculating rack systems. We tested the efficacy of BC tanks compared to open tanks (OT) and traditional mesh TIs using Poecilia parae, a live-bearing species that is notorious for intense filial cannibalism. In short-term trials, BC tanks yielded significantly higher initial fry counts on day 0 and day 2, while mesh inserts performed no better than OT. Over 10 days, negative binomial generalized linear models predicted substantial progressive fry loss in OT (∼60% decline) and inserts (∼68% decline), but no significant change in BC, consistent with high fry survival. These results provide the first quantitative evidence that automated water-flow separation of fry dramatically reduces filial cannibalism in live-bearing fishes. The BC design offers a scalable, low-cost, and humane method for fry recovery, reducing technician effort, minimizing animal stress, and lowering barriers for research and breeding programs involving live-bearing fishes (e.g., swordtails, mollies, Poeciliopsis, mosquitofish, halfbeaks, etc.).","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"21-26"},"PeriodicalIF":1.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13051513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146159820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision Dosing in Zebrafish: An Anesthesia-Free Oral Gavage for Reproductive Toxicity Assessment in Zebrafish. 斑马鱼的精确给药：一种用于斑马鱼生殖毒性评估的无麻醉口服灌胃。

IF 1.2

Zebrafish Pub Date : 2026-02-01 Epub Date: 2026-02-23 DOI: 10.1177/15458547251401445

Helena Sarret-Casellas, Laia Mejías, Samuel Jiménez, Sofía Barreiro, Lucía Lozano-White, Vincenzo Di Donato, Christian Cortés-Campos

引用次数: 0

Mapping the Landscape: A Comprehensive Analysis of Zebrafish Research in Brazil (2020-2025). 绘制景观：对巴西斑马鱼研究的综合分析（2020-2025）。

IF 1.2

Zebrafish Pub Date : 2026-02-01 Epub Date: 2026-02-09 DOI: 10.1177/15458547261420783

Monica Lopes-Ferreira, Daniela Coelho Cunha, Darlan Gusso, Manuela Jacobina Sa Mj, Felipe Justiniano Pinto, Carla Lima

{"title":"Mapping the Landscape: A Comprehensive Analysis of Zebrafish Research in Brazil (2020-2025).","authors":"Monica Lopes-Ferreira, Daniela Coelho Cunha, Darlan Gusso, Manuela Jacobina Sa Mj, Felipe Justiniano Pinto, Carla Lima","doi":"10.1177/15458547261420783","DOIUrl":"10.1177/15458547261420783","url":null,"abstract":"Brazil has emerged as a significant contributor to the global zebrafish (Danio rerio) research community, yet a comprehensive analysis of its national output, collaboration networks, and thematic focus has been lacking. This study provides a systematic bibliometric analysis of 801 Brazilian-corresponding articles published from 2020 to 2025, representing ∼2.7% of global production. Our findings reveal a marked concentration of scientific output, with the South and Southeast regions contributing ∼65% of national publications, led by the states of Rio Grande do Sul (24.8%) and São Paulo (20.4%). Despite this geographic disparity, a robust and integrative national collaboration network connects all regions, with an average 34% of publications involving interstate co-authorship. International partnerships are substantial (30.4% of output), led by the Southeast and South regions, and feature distinct geographic profiles, including the Central-West's links with South Asia. The field is characterized by a strong applied focus, with Toxicology (34% of studies), Pharmacology (18.3%), and Neuroscience (15%) dominating the research landscape, aligning with the predominant use of the adult zebrafish model (64%). Publication is heavily concentrated in environmental and toxicology journals, with nearly half of all output published by Elsevier (47%). These results map a dynamic, collaborative, and thematically focused national research community that is resilient yet faces persistent regional inequalities. The study establishes a critical baseline for understanding the structure and drivers of Brazilian science in a globally relevant model organism.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"10-20"},"PeriodicalIF":1.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146145138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cobalt Chloride-Induced Tissue Regeneration and Wound Healing Depend on HIF-1α and VEGF-A-Mediated Neovascularization. 氯化钴诱导的组织再生和伤口愈合依赖于HIF-1α和vegf - a介导的新生血管。

IF 1.2

Zebrafish Pub Date : 2025-12-01 Epub Date: 2025-10-15 DOI: 10.1177/15458547251383496

Vivek Sagayaraj Rathinasamy, Navina Paneerselvan, Sankar Jagadeeshan, Raskin Rajagopal Erusan, Reji Manjunathan

{"title":"Cobalt Chloride-Induced Tissue Regeneration and Wound Healing Depend on HIF-1α and VEGF-A-Mediated Neovascularization.","authors":"Vivek Sagayaraj Rathinasamy, Navina Paneerselvan, Sankar Jagadeeshan, Raskin Rajagopal Erusan, Reji Manjunathan","doi":"10.1177/15458547251383496","DOIUrl":"10.1177/15458547251383496","url":null,"abstract":"The study investigates the role of cobalt chloride (CoCl2), a hypoxia-inducing agent, in promoting tissue regeneration using zebrafish as a model system. Caudal fins of adult zebrafish were amputated and transdermally exposed to 1% CoCl2. The extent of fin regeneration and the neovascularization process at the growth front were analyzed. CoCl2 exposure significantly enhanced regeneration compared with controls, with increased fin length and more prominent blood vessel sprouting and anastomosis. Molecular and proteomics analyses revealed an upregulation of angiogenic and pro-angiogenic factors, particularly Vascular Endothelial Growth Factor (VEGF). To verify the role of VEGF in CoCl2-mediated tissue regeneration, the amputated fins were exposed to inhibitors such as genistein and SU5416. These results suggest that CoCl2 promotes tissue regrowth and wound healing by stimulating angiogenesis. The findings highlight the therapeutic potential of CoCl2 in enhancing regeneration and wound repair through the HIF-1α/VEGF signaling pathway, with potential implications for treating ischemic wounds.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"208-217"},"PeriodicalIF":1.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145305083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal Dynamics of c-Fos Expression in a Zebrafish Larva Model of Pentylenetetrazole-Induced Seizures. 戊四唑诱发癫痫的斑马鱼幼体模型中c-Fos表达的时间动态。

IF 1.2

Zebrafish Pub Date : 2025-12-01 DOI: 10.1177/15458547251401472

Amit Kumar, Damanpreet Singh

{"title":"Temporal Dynamics of c-Fos Expression in a Zebrafish Larva Model of Pentylenetetrazole-Induced Seizures.","authors":"Amit Kumar, Damanpreet Singh","doi":"10.1177/15458547251401472","DOIUrl":"https://doi.org/10.1177/15458547251401472","url":null,"abstract":"The use of zebrafish (Danio rerio) larva as an experimental model has gained a lot of interest in epilepsy research due to its multiple advantages over mammalian models. The present study investigated the time-dependent expression of c-fos, an immediate early gene, and a marker of neuronal activation, following pentylenetetrazole (PTZ)-induced seizures in zebrafish larvae at 7-day post-fertilization . The larvae were exposed to 8 mM PTZ for a 15-min period, transferred to fish system water, and processed for c-Fos expression analysis at 15, 30, 45, 60, and 90 min of the start of the experiment. c-fos mRNA and c-Fos protein levels were quantified, and Pearson correlation analysis was conducted to assess their relationship. PTZ exposure induced seizure-like behavior and resulted in a dynamic temporal expression of c-Fos, with both mRNA and protein achieving peak levels at 45 min and declining by 90 min. This approach applied a fixed exposure duration and defined post-exposure time points, which allowed a more accurate temporal profiling. The observed peak expression at 45 min suggested an optimal window for evaluating c-Fos expression in the PTZ-induced seizures model of zebrafish larva. These findings provided a valuable reference for selecting experimental endpoints in zebrafish larva seizure studies and enhanced the reliability of c-Fos as a marker of neuronal activation.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"22 6","pages":"223-229"},"PeriodicalIF":1.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145688851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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