Zebrafish最新文献

Understanding Rigor Mortis Impacts on Zebrafish Gamete Viability. 了解僵死对斑马鱼配子活力的影响。

Zebrafish Pub Date : 2025-02-27 DOI: 10.1089/zeb.2024.0177

Renata Villar Dantas, Lis Santos Marques, Thaiza Rodrigues Freitas, Nathalia Dos Santos Teixeira, Rômulo Batista Rodrigues, Jhony Lisbôa Benato, Raquel Santos Dos Santos, Diógenes Henrique Siqueira Silva, Danilo Pedro Streit

{"title":"Understanding Rigor Mortis Impacts on Zebrafish Gamete Viability.","authors":"Renata Villar Dantas, Lis Santos Marques, Thaiza Rodrigues Freitas, Nathalia Dos Santos Teixeira, Rômulo Batista Rodrigues, Jhony Lisbôa Benato, Raquel Santos Dos Santos, Diógenes Henrique Siqueira Silva, Danilo Pedro Streit","doi":"10.1089/zeb.2024.0177","DOIUrl":"https://doi.org/10.1089/zeb.2024.0177","url":null,"abstract":"This study aimed to evaluate the viability of gametes in zebrafish (Danio rerio), at different rigor mortis stages. Viability assessments were conducted on oocytes at various developmental stages using LIVE/DEAD and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. For sperm evaluation, both kinetic (computer-assisted sperm analysis) and morphological assessments (Rose Bengal staining) were performed. Results demonstrated that rigor mortis progression significantly impacted oocyte viability during post-rigor stages, with the following viability rates: pre-rigor (70.43 ± 12.31%), fresh/control (46.43 ± 12.54%), post-rigor (27.62 ± 22.29%), and rigor mortis (comparable to fresh/control). Conversely, sperm kinetics exhibited nuanced responses to the rigor mortis stages, with specific parameters showing sensitivity, whereas the others remained relatively stable. Sperm motility was higher in the fresh/control (63.23 ± 19.03%) and pre-rigor (58.96 ± 14.38%) compared to the post-rigor group (3.34 ± 4.65%). This study highlights the significance of the pre-rigor for successful gamete collection and preservation. These findings provide valuable insights for conservation efforts and optimization of genetic resource management for endangered fish species. This study aimed to develop effective assistive reproductive techniques by elucidating the interplay between rigor mortis and gamete quality, contributing to the broader goals of species conservation and maintenance of genetic diversity in fish populations.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143525759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small Fishes, Big Issues: Species Delimitation in Hemigrammus Marginatus, Gill, 1958 (Acestrorhamphidae: Pristellinae) from Brazilian Coastal Basins Based on Integrative Genetics. 小鱼类，大问题：基于整合遗传学的巴西沿海流域 Hemigrammus Marginatus, Gill, 1958 (Acestrorhamphidae: Pristellinae) 鱼种划分。

Zebrafish Pub Date : 2025-02-14 DOI: 10.1089/zeb.2024.0174

Mauricio B Fernandes, Jamille De Araújo Bitencourt, André Teixeira Da Silva, Marcelo Ricardo Vicari, Matheus Azambuja, Paulo Roberto Antunes De Mello Affonso

{"title":"Small Fishes, Big Issues: Species Delimitation in Hemigrammus Marginatus, Gill, 1958 (Acestrorhamphidae: Pristellinae) from Brazilian Coastal Basins Based on Integrative Genetics.","authors":"Mauricio B Fernandes, Jamille De Araújo Bitencourt, André Teixeira Da Silva, Marcelo Ricardo Vicari, Matheus Azambuja, Paulo Roberto Antunes De Mello Affonso","doi":"10.1089/zeb.2024.0174","DOIUrl":"https://doi.org/10.1089/zeb.2024.0174","url":null,"abstract":"The small characins represent a systematic puzzle in the Neotropical ichthyofauna as a result of independent miniaturization processes, adaptive convergence and lack of diagnostic characters for several genera. In order to diminish the taxonomic uncertainties and the evolutionary pathways in Hemigrammus, we carried out an integrative genetic analysis in the putatively widespread Hemigrammus marginatus Ellis, 1958 by combining cytogenetic and molecular data based on the mitochondrial Cytochrome C Oxidase subunit I (COI). Specimens of H. marginatus from the type locality in Itapicuru River basin and other two populations from coastal rivers in northeastern Brazil were analyzed and compared with the available data from other regions in South America. Conspicuous macro and microkaryotypic differences were detected between the samples from northeastern and southern Brazil (Upper Paraná River basin). Likewise, the DNA barcoding and species delimitation analyses recovered distinct Molecular Operational Taxonomical Units within H. marginatus. Therefore, the population from the type locality should be referred to as H. marginatus stricto sensu, representing a restricted characin taxon from coastal drainages (including the São Francisco River basin) along northeastern Brazil, while other populations of this small characin fish need to be taxonomically revised and managed as unique lineages.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143417133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Food Delivery Method for Learning Studies Detects Significant Differences in Food Preference in Zebrafish.

Zebrafish Pub Date : 2025-02-01 Epub Date: 2025-02-06 DOI: 10.1089/zeb.2024.0176

Amira Abozaid, Melissa Scaramuzzo, Hemali Patel, Robert Gerlai

{"title":"A Novel Food Delivery Method for Learning Studies Detects Significant Differences in Food Preference in Zebrafish.","authors":"Amira Abozaid, Melissa Scaramuzzo, Hemali Patel, Robert Gerlai","doi":"10.1089/zeb.2024.0176","DOIUrl":"10.1089/zeb.2024.0176","url":null,"abstract":"Despite decades of research with laboratory rodents, the mechanisms underlying learning and memory, and their impairment, are still not fully understood. The zebrafish is a newcomer in this research area, but it has shown great promise. Food is often employed as a reinforcer in learning tasks with rodents. However, for zebrafish, food has been a problematic reinforcer. Controlling timing and localization of its delivery is difficult. What food types zebrafish prefer is also rarely studied? Here, we describe a novel food delivery hardware and procedure. The apparatus is simple, cheap to manufacture, and easy to employ. Using this new method, we compare how zebrafish respond to three food types, artemia nauplii, crushed tropical fish flakes, and small zebrafish pellets. In binary choice tasks, we show that zebrafish spend significantly more time near the artemia delivery cylinder, swim closer to, and visit this cylinder more frequently compared to food cylinders delivering flakes or pellets, while responses to these latter two cylinders do not differ from each other. We conclude that the newly developed method allows the quantification of food preference in zebrafish, and that it will lead to the identification of highly rewarding food types for learning studies in this species.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"20-29"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgment of Reviewers 2024.

Zebrafish Pub Date : 2025-02-01 DOI: 10.1089/zeb.2024.58862.revack

引用次数: 0

An Image Processing Tool for Automated Quantification of Bacterial Burdens in Zebrafish Larvae. 用于斑马鱼幼虫细菌负荷自动定量的图像处理工具。

Zebrafish Pub Date : 2025-02-01 Epub Date: 2024-12-24 DOI: 10.1089/zeb.2024.0170

Naoya Yamaguchi, Hideo Otsuna, Michal Eisenberg-Bord, Lalita Ramakrishnan

引用次数: 0

An Automated Low-Cost Solution for Creating a Multiple-Step Thermal Gradient to Record Daily Fish Thermoregulatory Behavior. 一种自动化的低成本解决方案，用于创建多步热梯度来记录鱼类的日常温度调节行为。

Zebrafish Pub Date : 2025-02-01 Epub Date: 2025-02-06 DOI: 10.1089/zeb.2024.0153

Francesca Conti, Gonzalo de Alba, Jose F López-Olmeda, Luisa M Vera, Gregory Lucon-Xiccato, Elena Mainardi, Silvia Cesari, Michele Bottarelli, Cristiano Bertolucci, Francisco J Sánchez-Vázquez, Elia Gatto

引用次数: 0

In Vivo Measurement of Intestinal Permeability to Macromolecules in Adult Zebrafish (Danio rerio).

Zebrafish Pub Date : 2025-02-01 Epub Date: 2025-02-03 DOI: 10.1089/zeb.2024.0167

Marcela G Meirelles, Camila I M Fénero, Bruna F Nornberg, Niels Olsen S Camara, Luis Fernando Marins

{"title":"In Vivo Measurement of Intestinal Permeability to Macromolecules in Adult Zebrafish (Danio rerio).","authors":"Marcela G Meirelles, Camila I M Fénero, Bruna F Nornberg, Niels Olsen S Camara, Luis Fernando Marins","doi":"10.1089/zeb.2024.0167","DOIUrl":"10.1089/zeb.2024.0167","url":null,"abstract":"Intestinal permeability plays a crucial role in intestinal barrier function. Altered intestinal permeability is well documented in numerous chronic diseases and may serve as a risk factor for disease onset as well as a target for innovative therapeutic strategies. While reliable and sensitive approaches for studying intestinal permeability have been established in animal models, such as mice and zebrafish larvae, methods for investigating this in adult zebrafish remain a considerable challenge. The zebrafish has emerged as a valuable model for studying intestinal development, physiology, and disease. Moreover, zebrafish offer certain advantages over rodent models, such as the ability to evaluate the dynamic interactions of labeled markers in vivo and in real time. In this study, we present a comprehensive pipeline for assessing in vivo intestinal permeability in adult zebrafish using fluorescent-labeled dextran. Detailed protocols for fish handling, reagent preparation, optimization of reagent dosage and delivery routes, and quantification of fluorescent markers in extraintestinal sites are provided. Our findings suggest that zebrafish hold promise as an alternative model for in vivo investigations of intestinal permeability induced by genetic, pathophysiological, and/or pharmacological events.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Zpr-3 Antibody Recognizes the 320-354 Region of Rho and Labels Both Rods and Green Cones in Zebrafish. Zpr-3 抗体能识别 Rho 的 320-354 区域，并能标记斑马鱼的杆和绿锥体。

Zebrafish Pub Date : 2024-12-01 Epub Date: 2024-09-24 DOI: 10.1089/zeb.2024.0159

Hualei Hu, Yayun Qin, Zhen Qu, Yuwen Huang, Xiang Ren, Mugen Liu, Fei Liu, Pan Gao

引用次数: 0

Zebrafish as a Model Organism for Congenital Hydrocephalus: Characteristics and Insights. 斑马鱼作为先天性脑积水的模式生物：特征与启示。

Zebrafish Pub Date : 2024-12-01 Epub Date: 2024-11-07 DOI: 10.1089/zeb.2024.0148

Kaiyue Wang, Zhi Tang, Yijian Yang, Yating Guo, Zhikun Liu, Zhangjie Su, Xuejun Li, Gelei Xiao

{"title":"Zebrafish as a Model Organism for Congenital Hydrocephalus: Characteristics and Insights.","authors":"Kaiyue Wang, Zhi Tang, Yijian Yang, Yating Guo, Zhikun Liu, Zhangjie Su, Xuejun Li, Gelei Xiao","doi":"10.1089/zeb.2024.0148","DOIUrl":"10.1089/zeb.2024.0148","url":null,"abstract":"Hydrocephalus is a cerebrospinal fluid-related disease that usually manifests as abnormal dilation of the ventricles, with a triad of clinical findings including walking difficulty, reduced attention span, and urinary frequency or incontinence. The onset of congenital hydrocephalus is closely related to mutations in genes that regulate brain development. Currently, our understanding of the mechanisms of congenital hydrocephalus remains limited, and the prognosis of existing treatments is unsatisfactory. Additionally, there are no suitable or dedicated model organisms for congenital hydrocephalus. Therefore, it is significant to determine the mechanism and develop special animal models of congenital hydrocephalus. Recently, zebrafish have emerged as a popular model organism in many fields, including developmental biology, genetics, and toxicology. Its genome shares high similarity with that of humans, and it has fast and low-cost reproduction. These advantages make it suitable for studying the pathogenesis and therapeutic approaches for various diseases, specifically congenital diseases. This study explored the possibility of using zebrafish as a model organism for congenital hydrocephalus. This review describes the characteristics of zebrafish and discusses specific congenital hydrocephalus models. The advantages and limitations of using zebrafish for hydrocephalus research are highlighted, and insights for further model development are provided.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"361-384"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing Zebrafish Husbandry: Takeaways From the 2024 Husbandry Workshop and Husbandry Summit. 推进斑马鱼饲养：2024 年饲养研讨会和饲养峰会的收获。

Zebrafish Pub Date : 2024-12-01 Epub Date: 2024-10-02 DOI: 10.1089/zeb.2024.0149

Michelle Altemara, Bobbi Baur, Zoltan M Varga

{"title":"Advancing Zebrafish Husbandry: Takeaways From the 2024 Husbandry Workshop and Husbandry Summit.","authors":"Michelle Altemara, Bobbi Baur, Zoltan M Varga","doi":"10.1089/zeb.2024.0149","DOIUrl":"10.1089/zeb.2024.0149","url":null,"abstract":"The 2024 Zebrafish Husbandry Workshop and Summit held during the World Aquaculture Society Meeting in San Antonio focused on key areas for improving zebrafish husbandry research. Discussions highlighted the need for comprehensive literature on husbandry, better communication and collaboration between researchers and facility staff, and the adoption of a standardized reference diet. Current literature lacks comprehensive data and often overlooks crucial factors such as housing density and space requirements for fish development. Collaborative efforts between researchers and facility managers are essential for acquiring accurate husbandry data and minimizing pathogen risks. Standardizing descriptive language and parameter lists in publications and enhancing communication between facilities can improve research quality. Action items proposed include better communication of incoming fish information, standardization of pathogen monitors, transparency in husbandry practices, and fostering a spirit of collaboration among organizations. The summit emphasized the importance of increased PI awareness about husbandry, testing existing standardized diets, forming consortia to oversee diet standardization, creating unified repositories and forums, and conducting evidence-based husbandry studies.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"418-422"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142368143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0