在水中不注射添加膜孕激素受体激动剂使斑马鱼交配的新方法。

Zebrafish Pub Date : 2025-06-11 DOI:10.1089/zeb.2025.0020
Saokat Ahamed, Md Abul Bashar, Mohammad Maksudul Hassan, Toshinobu Tokumoto
{"title":"在水中不注射添加膜孕激素受体激动剂使斑马鱼交配的新方法。","authors":"Saokat Ahamed, Md Abul Bashar, Mohammad Maksudul Hassan, Toshinobu Tokumoto","doi":"10.1089/zeb.2025.0020","DOIUrl":null,"url":null,"abstract":"<p><p>This study investigates a novel method to induce mating in zebrafish (<i>Danio rerio</i>) by direct water addition of membrane progestin receptor (mPR) agonists, Org OD 02-0 (Org), or a maturation-inducing steroid, 17α,20β-dihydroxy-4-pregnen-3-one (DHP). Traditional spawning induction methods using hormone injections can be invasive and stressful for fish, especially small fish such as zebrafish. A previous study showed that progestins applied externally to the water can induce oocyte maturation and ovulation in female zebrafish. Then, a recent study showed that the addition of steroids and Org to the water also induced male sexual behavior in zebrafish. Therefore, it was hypothesized that the addition of these compounds to the water could induce mating in zebrafish. To address this, we investigated the efficacy of adding these compounds to the water in the vessel with a pair of zebrafish. Female and male zebrafish pairs were exposed to varying concentrations of DHP or Org in the water, and the resulting spawning rates and rate of sexual behavior induction were recorded. Our results showed that both simple addition of DHP and Org solution successfully induced mating within 4 h. A single chemical induced both spawning in females and sexual behavior in males. In this study, we have established a simple, novel method to induce mating in zebrafish by adding mPR agonists to the water.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Novel Method to Make Zebrafish Mate by Adding an Membrane Progestin Receptor Agonist to the Water Without Injection.\",\"authors\":\"Saokat Ahamed, Md Abul Bashar, Mohammad Maksudul Hassan, Toshinobu Tokumoto\",\"doi\":\"10.1089/zeb.2025.0020\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study investigates a novel method to induce mating in zebrafish (<i>Danio rerio</i>) by direct water addition of membrane progestin receptor (mPR) agonists, Org OD 02-0 (Org), or a maturation-inducing steroid, 17α,20β-dihydroxy-4-pregnen-3-one (DHP). Traditional spawning induction methods using hormone injections can be invasive and stressful for fish, especially small fish such as zebrafish. A previous study showed that progestins applied externally to the water can induce oocyte maturation and ovulation in female zebrafish. Then, a recent study showed that the addition of steroids and Org to the water also induced male sexual behavior in zebrafish. Therefore, it was hypothesized that the addition of these compounds to the water could induce mating in zebrafish. To address this, we investigated the efficacy of adding these compounds to the water in the vessel with a pair of zebrafish. Female and male zebrafish pairs were exposed to varying concentrations of DHP or Org in the water, and the resulting spawning rates and rate of sexual behavior induction were recorded. Our results showed that both simple addition of DHP and Org solution successfully induced mating within 4 h. A single chemical induced both spawning in females and sexual behavior in males. In this study, we have established a simple, novel method to induce mating in zebrafish by adding mPR agonists to the water.</p>\",\"PeriodicalId\":94273,\"journal\":{\"name\":\"Zebrafish\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-06-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zebrafish\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1089/zeb.2025.0020\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zebrafish","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/zeb.2025.0020","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

本研究研究了一种诱导斑马鱼(Danio rerio)交配的新方法,即直接在水中加入膜孕激素受体(mPR)激动剂Org OD 02-0 (Org)或诱导成熟的类固醇17α,20β-二羟基-4-孕-3-one (DHP)。传统的使用激素注射的诱导产卵方法对鱼类,特别是像斑马鱼这样的小鱼来说,可能是侵入性的和有压力的。先前的研究表明,在水中外用黄体酮可以诱导雌斑马鱼卵母细胞成熟和排卵。然后,最近的一项研究表明,在水中添加类固醇和Org也会诱导斑马鱼的雄性性行为。因此,假设在水中添加这些化合物可以诱导斑马鱼交配。为了解决这个问题,我们用一对斑马鱼研究了将这些化合物添加到容器中的水中的功效。将雌性和雄性斑马鱼对暴露于不同浓度的DHP或Org中,记录其产卵率和性行为诱导率。结果表明,简单地添加DHP和Org溶液都能在4小时内成功诱导交配。一种化学物质既能诱导雌性产卵,也能诱导雄性交配。在本研究中,我们建立了一种简单、新颖的方法,通过在水中添加mPR激动剂来诱导斑马鱼交配。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Novel Method to Make Zebrafish Mate by Adding an Membrane Progestin Receptor Agonist to the Water Without Injection.

This study investigates a novel method to induce mating in zebrafish (Danio rerio) by direct water addition of membrane progestin receptor (mPR) agonists, Org OD 02-0 (Org), or a maturation-inducing steroid, 17α,20β-dihydroxy-4-pregnen-3-one (DHP). Traditional spawning induction methods using hormone injections can be invasive and stressful for fish, especially small fish such as zebrafish. A previous study showed that progestins applied externally to the water can induce oocyte maturation and ovulation in female zebrafish. Then, a recent study showed that the addition of steroids and Org to the water also induced male sexual behavior in zebrafish. Therefore, it was hypothesized that the addition of these compounds to the water could induce mating in zebrafish. To address this, we investigated the efficacy of adding these compounds to the water in the vessel with a pair of zebrafish. Female and male zebrafish pairs were exposed to varying concentrations of DHP or Org in the water, and the resulting spawning rates and rate of sexual behavior induction were recorded. Our results showed that both simple addition of DHP and Org solution successfully induced mating within 4 h. A single chemical induced both spawning in females and sexual behavior in males. In this study, we have established a simple, novel method to induce mating in zebrafish by adding mPR agonists to the water.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信