Saokat Ahamed, Md Abul Bashar, Mohammad Maksudul Hassan, Toshinobu Tokumoto
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引用次数: 0
摘要
本研究研究了一种诱导斑马鱼(Danio rerio)交配的新方法,即直接在水中加入膜孕激素受体(mPR)激动剂Org OD 02-0 (Org)或诱导成熟的类固醇17α,20β-二羟基-4-孕-3-one (DHP)。传统的使用激素注射的诱导产卵方法对鱼类,特别是像斑马鱼这样的小鱼来说,可能是侵入性的和有压力的。先前的研究表明,在水中外用黄体酮可以诱导雌斑马鱼卵母细胞成熟和排卵。然后,最近的一项研究表明,在水中添加类固醇和Org也会诱导斑马鱼的雄性性行为。因此,假设在水中添加这些化合物可以诱导斑马鱼交配。为了解决这个问题,我们用一对斑马鱼研究了将这些化合物添加到容器中的水中的功效。将雌性和雄性斑马鱼对暴露于不同浓度的DHP或Org中,记录其产卵率和性行为诱导率。结果表明,简单地添加DHP和Org溶液都能在4小时内成功诱导交配。一种化学物质既能诱导雌性产卵,也能诱导雄性交配。在本研究中,我们建立了一种简单、新颖的方法,通过在水中添加mPR激动剂来诱导斑马鱼交配。
A Novel Method to Make Zebrafish Mate by Adding an Membrane Progestin Receptor Agonist to the Water Without Injection.
This study investigates a novel method to induce mating in zebrafish (Danio rerio) by direct water addition of membrane progestin receptor (mPR) agonists, Org OD 02-0 (Org), or a maturation-inducing steroid, 17α,20β-dihydroxy-4-pregnen-3-one (DHP). Traditional spawning induction methods using hormone injections can be invasive and stressful for fish, especially small fish such as zebrafish. A previous study showed that progestins applied externally to the water can induce oocyte maturation and ovulation in female zebrafish. Then, a recent study showed that the addition of steroids and Org to the water also induced male sexual behavior in zebrafish. Therefore, it was hypothesized that the addition of these compounds to the water could induce mating in zebrafish. To address this, we investigated the efficacy of adding these compounds to the water in the vessel with a pair of zebrafish. Female and male zebrafish pairs were exposed to varying concentrations of DHP or Org in the water, and the resulting spawning rates and rate of sexual behavior induction were recorded. Our results showed that both simple addition of DHP and Org solution successfully induced mating within 4 h. A single chemical induced both spawning in females and sexual behavior in males. In this study, we have established a simple, novel method to induce mating in zebrafish by adding mPR agonists to the water.