Zebrafish最新文献

{"title":"U2 and U4 snDNA Comparative Chromosomal Mapping in the Neotropical Fish Genera <i>Apareiodon</i> and <i>Parodon</i> (Characiformes: Parodontidae).","authors":"Matheus Azambuja, Viviane Nogaroto, Orlando Moreira-Filho, Marcelo Ricardo Vicari","doi":"10.1089/zeb.2023.0025","DOIUrl":"10.1089/zeb.2023.0025","url":null,"abstract":"<p><p>Small nuclear DNA (snDNA) are valuable cytogenetic markers for comparative studies in chromosome evolution because different distribution patterns were found among species. Parodontidae, a Neotropical fish family, is known to have female heterogametic sex chromosome systems in some species. The U2 and U4 snDNA sites have been found to be involved in Z and W chromosome differentiation in <i>Apareiodon</i> sp., <i>Apareiodon affinis</i>, and <i>Parodon hilarii</i>. However, few studies have evaluated snDNA sites as propulsors of chromosome diversification among closely related fish species. In this study, we investigated the distribution of U2 and U4 snDNA clusters in the chromosomes of 10 populations/species belonging to <i>Apareiodon</i> and <i>Parodon</i>, aiming to identify chromosomal homeologies or diversification. <i>In situ</i> localization data revealed a submetacentric pair carrying the U2 snDNA site among the populations/species analyzed. Furthermore, all studied species demonstrated homeology in the location of U4 snDNA cluster in the proximal region of metacentric pair 1, besides an additional signal showing up with a divergence in <i>Apareiodon</i>. Comparative chromosomal mapping of U4 snDNA also helped to reinforce the proposal of the ZZ/ZW₁W₂ sex chromosome system origin in an <i>A. affinis</i> population. According to cytogenetic data, the study corroborates the diversification in Parodontidae paired species with uncertain taxonomy.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"221-228"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41158053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functioning and Gene Expression of Adenosine A₁ Receptor During Zebrafish (<i>Danio rerio</i>) Development.","authors":"Giovanna Trevisan Couto, Higor Arruda Caetano, Guilherme Pietro da Silva, Liliana Rockenbach, Jéssica Scheid da Silva, Monica Ryff Vianna, Rosane Souza Da Silva","doi":"10.1089/zeb.2023.0002","DOIUrl":"10.1089/zeb.2023.0002","url":null,"abstract":"<p><p>The A1 adenosine receptor is the most widely expressed P1 receptor in vertebrates, performing inhibitory tone of the nervous system. Increased levels of adenosine are crucial to promote tissue protection in threatening situations, such as convulsion and hypoxia. Zebrafish is an established model organism for studies on health and disease. In this study, we evaluated the functionality of A₁ adenosine receptor through development of zebrafish (6-7-day-, 3-, 8-, and 24-month-old), assessing: (I) the effects of the agonist N₆-cyclopenthyladenosine (CPA) over locomotor parameters, (II) the anticonvulsant properties of CPA and adenosine <i>per se</i> in the pentylenetetrazol-induced seizure, and (III) the gene expression of <i>adora1b</i> through development. CPA promoted decreased distance traveled in the highest concentrations/doses tested (larvae: 75 to 500 μM; adults: 20 mg.kg^-1), altered mean velocity (larvae: 50-500 μM; adults: 20 mg.kg^-1) and time in the bottom zone of apparatus (adults: decrease in 20 mg.kg^-1). Adenosine increased the latency of the larvae to reach stage II at 5 and 10 μM. CPA anticonvulsant effect against convulsive stage II was reached at 75 μM, although it decreased basal locomotor activity in larvae. For adults, CPA 10 mg.kg^-1 was effective as anticonvulsant without locomotory effects. Adenosine had minor anticonvulsant effects in the concentration tested (larvae: 5 and 10 μM). The level of gene expression of <i>adora1b</i> was stable in brain from adult animals (8- and 24-month-old animals). These results suggest that zebrafish has similar responses to CPA as mammals. To avoid confounding factors, such as locomotor effects, during any brain function investigation using A₁ adenosine receptor as a target, the concentration below 75 μM or below the dose of 20 mg.kg^-1 of CPA is ideal for zebrafish at larval and adult stages, respectively.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"20 5","pages":"210-220"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49687024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Four-Well Dish for High-Resolution Longitudinal Imaging of the Tail and Posterior Trunk of Larval Zebrafish.","authors":"Bart Weijts, Eugene Tkachenko, David Traver, Alex Groisman","doi":"10.1089/zeb.2016.1406","DOIUrl":"10.1089/zeb.2016.1406","url":null,"abstract":"<p><p>We describe the design, fabrication, and applications of a four-well dish for imaging of the trunk of larval zebrafish. The dish facilitates immobilization of anesthetized zebrafish larvae, with their tails gently pushed against a microscope cover glass, enabling longitudinal imaging at 24-72 h postfertilization using high-resolution objective lenses.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"14 5","pages":"489-491"},"PeriodicalIF":0.0,"publicationDate":"2017-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140295774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glyphosate Adversely Affects Danio rerio Males: Acetylcholinesterase Modulation and Oxidative Stress.","authors":"Fernanda Moreira Lopes, Sergiane Souza Caldas, Ednei Gilberto Primel, Carlos Eduardo da Rosa","doi":"10.1089/zeb.2016.1341","DOIUrl":"https://doi.org/10.1089/zeb.2016.1341","url":null,"abstract":"<p><p>It has been demonstrated that glyphosate-based herbicides are toxic to animals. In the present study, reactive oxygen species (ROS) generation, antioxidant capacity against peroxyl radicals (ACAP), and lipid peroxidation (LPO), as well as the activity and expression of the acetylcholinesterase (AChE) enzyme, were evaluated in Danio rerio males exposed to 5 or 10 mg/L of glyphosate for 24 and 96 h. An increase in ACAP in gills after 24 h was observed in the animals exposed to 5 mg/L of glyphosate. A decrease in LPO was observed in brain tissue of animals exposed to 10 mg/L after 24 h, while an increase was observed in muscle after 96 h. No significant alterations were observed in ROS generation. AChE activity was not altered in muscles or brains of animals exposed to either glyphosate concentration for 24 or 96 h. However, gene expression of this enzyme in the brain was reduced after 24 h and was enhanced in both brain and muscle tissues after 96 h. Thus, contrary to previous findings that had attributed the imbalance in the oxidative state of animals exposed to glyphosate-based herbicides to surfactants and other inert compounds, the present study demonstrated that glyphosate per se promotes this same effect in zebrafish males. Although glyphosate concentrations did not alter AChE activity, this study demonstrated for the first time that this molecule affects ache expression in male zebrafish D. rerio.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"14 2","pages":"97-105"},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/zeb.2016.1341","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138815091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}