Zebrafish最新文献_第7页

Effects of 4 Testing Arena Sizes and 11 Types of Embryo Media on Sensorimotor Behaviors in Wild-Type and chd7 Mutant Zebrafish Larvae. 4种测试场大小和11种胚胎培养基对野生型和chd7突变型斑马鱼幼体感官运动行为的影响

Zebrafish Pub Date : 2024-02-01 DOI: 10.1089/zeb.2023.0052

Dana R Hodorovich, Tiara Fryer Harris, Derek F Burton, Katie M Neese, Rachael A Bieler, Vimal Chudasama, Kurt C Marsden

{"title":"Effects of 4 Testing Arena Sizes and 11 Types of Embryo Media on Sensorimotor Behaviors in Wild-Type and chd7 Mutant Zebrafish Larvae.","authors":"Dana R Hodorovich, Tiara Fryer Harris, Derek F Burton, Katie M Neese, Rachael A Bieler, Vimal Chudasama, Kurt C Marsden","doi":"10.1089/zeb.2023.0052","DOIUrl":"10.1089/zeb.2023.0052","url":null,"abstract":"The larval zebrafish is a highly versatile model across research disciplines, and the expanding use of behavioral analysis has contributed to many advances in neuropsychiatric, developmental, and toxicological studies, often through large-scale chemical and genetic screens. In the absence of standardized approaches to larval zebrafish behavior analysis, however, it is critical to understand the impact on behavior of experimental variables such as the size of testing arenas and the choice of embryo medium. Using a custom-built, modular high-throughput testing system, we examined the effects of 4 testing arena sizes and 11 types of embryo media on conserved sensorimotor behaviors in zebrafish larvae. Our data show that testing arena size impacts acoustic startle sensitivity and kinematics, as well as spontaneous locomotion and thigmotaxis, with fish tested in larger arenas displaying reduced startle sensitivity and increased locomotion. We also find that embryo media can dramatically affect startle sensitivity, kinematics, habituation, and prepulse inhibition, as well as spontaneous swimming, turning, and overall activity. Common medium components such as methylene blue and high calcium concentration consistently reduced startle sensitivity and locomotion. To further address how the choice of embryo medium can impact phenotype expression in zebrafish models of disease, we reared chd7 mutant larvae, a model of CHARGE syndrome with previously characterized morphological and behavioral phenotypes, in five different types of media and observed impacts on all phenotypes. By defining the effects of these key extrinsic factors on larval zebrafish behavior, these data can help researchers select the most appropriate conditions for their specific research questions, particularly for genetic and chemical screens.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10902501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139674011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Status of the Zebrafish Euthanasia and Humane Endpoint in the Republic of Korea and Guideline Suggestion from Nationwide Expert Elicitation: A Model for Other Countries. 大韩民国斑马鱼安乐死和人道终点的现状及全国专家征询的指导建议：其他国家的典范。

Zebrafish Pub Date : 2024-02-01 DOI: 10.1089/zeb.2023.0086

Sang Wha Kim, Won Joon Jung, Seung Hee Han, Sun Kyeong Kim, Hyunju Ro, Heui Jin Kim, Ja Un Tae, Yi Rang Na, Seung Hyeok Seok

{"title":"Current Status of the Zebrafish Euthanasia and Humane Endpoint in the Republic of Korea and Guideline Suggestion from Nationwide Expert Elicitation: A Model for Other Countries.","authors":"Sang Wha Kim, Won Joon Jung, Seung Hee Han, Sun Kyeong Kim, Hyunju Ro, Heui Jin Kim, Ja Un Tae, Yi Rang Na, Seung Hyeok Seok","doi":"10.1089/zeb.2023.0086","DOIUrl":"10.1089/zeb.2023.0086","url":null,"abstract":"Since its introduction as a model organism in the 1980s, the use of zebrafish (Danio rerio) in research has expanded worldwide. Despite its now widespread use in research, guidelines to safeguard the ethical treatment of zebrafish, particularly with regard to euthanasia and humane endpoint practices, remain inadequate. One well-recognized example is the use of excess tricaine methanesulfonate (MS-222) as a means to euthanize zebrafish, regardless of life stage. In this study, through nationwide expert elicitation, we provide a detailed account of zebrafish research practices within the Republic of Korea and the challenges of implementing appropriate methods for euthanasia as a humane endpoint, with many opting for hypothermic shock. We report a local expert consensus for establishing national guidelines to improve zebrafish welfare and good research practice. Suggestions and recommendations for national guidelines were offered. Taken together, our findings raise awareness broadly among zebrafish research practitioners in the field, offer an accurate account of the welfare and treatment of zebrafish in research within the Republic of Korea, and advocate for the development and implementation of national guidelines. As such, our study is useful as a model to adopt the expert elicitation approach to investigate, quantify, and address welfare concerns in zebrafish research, and to establish best practice guidelines.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"21 1","pages":"53-66"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139914392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgment of Reviewers 2023. 鸣谢 2023 年审稿人。

Zebrafish Pub Date : 2024-02-01 Epub Date: 2023-12-26 DOI: 10.1089/zeb.2023.29011.ack

引用次数: 0

Correction to: In Vitro Storage of Functional Sperm at Room Temperature in Zebrafish and Medaka Zebrafish 2023;20(6):229-235; doi: 10.1089/zeb.2023.0054. 更正：斑马鱼和青鳉功能精子的室温体外贮存斑马鱼 2023; 20(6):229-235; doi: 10.1089/zeb.2023.0054.

Zebrafish Pub Date : 2024-02-01 DOI: 10.1089/zeb.2023.0054.correx

引用次数: 0

Chemical and Visual Cues as Modulators of the Stress Response to Social Isolation in the Marine Medaka, Oryzias melastigma. 化学和视觉线索是海鳉社会隔离应激反应的调节剂

Zebrafish Pub Date : 2024-02-01 DOI: 10.1089/zeb.2023.0046

Alexandre Lebel, Libin Zhang, David Gonçalves

{"title":"Chemical and Visual Cues as Modulators of the Stress Response to Social Isolation in the Marine Medaka, Oryzias melastigma.","authors":"Alexandre Lebel, Libin Zhang, David Gonçalves","doi":"10.1089/zeb.2023.0046","DOIUrl":"10.1089/zeb.2023.0046","url":null,"abstract":"The marine medaka is emerging as a potential behavioral model organism for ocean studies, namely on marine ecotoxicology. However, not much is known on the behavior of the species and behavioral assays lack standardization. This study assesses the marine medaka as a potential model for chemical communication. We investigated how short exposure to visual and chemical cues mediated the stress response to social isolation with the light/dark preference test (LDPT) and the open field test (OFT). After a 5-day isolation period, and 1 h before testing, isolated fish were randomly assigned to one of four groups: (1) placed in visual contact with conspecifics; (2) exposed to a flow of holding water from a group of conspecifics; (3) exposed to both visual and chemical cues from conspecifics; or (4) not exposed to any stimuli (controls). During the LDPT, the distance traveled and transitions between zones were more pronounced in animals exposed to the conspecific's chemical stimuli. The time spent in each area did not differ between the groups, but a clear preference for the bright area in all animals indicates robust phototaxis. During the OFT, animals exposed only to chemical cues initially traveled more than those exposed to visual or both stimuli, and displayed lower thigmotaxis. Taken together, results show that chemical cues play a significant role in exploratory behavior in this species and confirm the LDPT and OFT as suitable tests for investigating chemical communication in this species.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"21 1","pages":"15-27"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139914391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Open-Source Programmable Interface for Sensory-Motor Biotests with Zebrafish (Danio rerio). 用于斑马鱼（Danio rerio）感官-运动生物测试的开源可编程界面。

Zebrafish Pub Date : 2024-02-01 Epub Date: 2024-01-09 DOI: 10.1089/zeb.2023.0067

Yutao Bai, Jason Henry, Florian Kreuder, David Mawdsley, Jan Kaslin, Donald Wlodkowic

引用次数: 0

Hidden Diversity in Eigenmannia (Gymnotiformes: Sternopygidae) Populations from Upper Paraná and Paraguay Rivers. 来自巴拉那河上游和巴拉圭河的 Eigenmannia（钩形目：Sternopygidae）种群的隐藏多样性。

Zebrafish Pub Date : 2024-02-01 Epub Date: 2024-01-29 DOI: 10.1089/zeb.2023.0107

Raissa Caroline Mazetto, Laura Ivana Ramos, Margarida Maria de Rossi Vieira, Fernando Rogério Carvalho, Luciana Andréia Borin de Carvalho, Alessandra Valéria de Oliveira, Carlos Alexandre Fernandes, Ana Luiza de Brito Portela Castro

{"title":"Hidden Diversity in Eigenmannia (Gymnotiformes: Sternopygidae) Populations from Upper Paraná and Paraguay Rivers.","authors":"Raissa Caroline Mazetto, Laura Ivana Ramos, Margarida Maria de Rossi Vieira, Fernando Rogério Carvalho, Luciana Andréia Borin de Carvalho, Alessandra Valéria de Oliveira, Carlos Alexandre Fernandes, Ana Luiza de Brito Portela Castro","doi":"10.1089/zeb.2023.0107","DOIUrl":"10.1089/zeb.2023.0107","url":null,"abstract":"Eigenmannia is a highly diverse genus within the Sternopygidae family, comprising 30 species. Due to its complex taxonomy, molecular analyses have been crucial for species delimitation within this group. Therefore, the present study presents a genetic analysis using sequences of the mitochondrial gene cytochrome c oxidase subunit 1 (COI) in specimens previously identified through alpha taxonomy as E. correntes (with unpublished data), E. virescens, and E. trilineata, originating from various locations within the Upper Paraná and Paraguay River basins in Brazil. The molecular data confirm the taxonomic complexity of the genus, as individuals morphologically identified as E. virescens and E. trilineata shared the same haplotype (H52). Furthermore, the results of the species delimitation tests suggest that specimens morphologically identified as E. virescens belong to the species E. trilineata. In addition, samples morphologically identified as E. correntes may correspond to more than one Operational Taxonomic Units (OTUs). Furthermore, the intraspecific Kimura-2-parameter (K2P) distances within the different studied populations are significant. This study has contributed valuable information about genetic diversity in Eigenmannia, emphasizing the importance of using integrative analyses to resolve taxonomic conflicts within the group. It also supports biogeographical studies and assists in biodiversity conservation efforts.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"39-47"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139571606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Affordable and Easy-to-Construct Zebrafish Housing System for Stable Long-Term Laboratory Research. 一种经济实惠且易于建造的斑马鱼住房系统，用于稳定的长期实验室研究。

Zebrafish Pub Date : 2023-12-01 Epub Date: 2023-11-22 DOI: 10.1089/zeb.2023.0061

Brian Lipscomb, Nicholas Seymour, Jada Lewis, Danny C LeBert

{"title":"An Affordable and Easy-to-Construct Zebrafish Housing System for Stable Long-Term Laboratory Research.","authors":"Brian Lipscomb, Nicholas Seymour, Jada Lewis, Danny C LeBert","doi":"10.1089/zeb.2023.0061","DOIUrl":"10.1089/zeb.2023.0061","url":null,"abstract":"Zebrafish have become a go-to model organism for in vivo studies, in part because of their reputation as being inexpensive to rear and house. Multiple do-it-yourself designs are currently available that provide laboratories with cost-effective housing systems. Unfortunately, these designs suffer from a range of issues ranging from poor water cycling rates and fragile housing tanks to inconsistent water conditions and designs that are prohibitively expensive for smaller laboratories to construct and maintain. These issues cause many of these housing systems to fall far short of the quality of commercially available zebrafish housing facilities. In this article, we present a novel, affordable, and easy-to-construct zebrafish housing system that improves upon previously published systems. The system utilizes three-dimensional printing technology to construct adaptable zebrafish tanks allowing for the housing of zebrafish at any stage of development. In addition, the water recirculation system utilizes multiple layers of filtration and no chemical adhesives, which allows for stable, long-term, housing of zebrafish in conditions suitable for research and teaching laboratories. The build described herein has been used by our laboratory to house zebrafish for over 3 years, representing multiple generations of housed fish.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"260-270"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138447723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tenth Aquatic Models of Human Disease Conference 2022 Workshop Report: Aquatics Nutrition and Reference Diet Development. 第十届人类疾病水生模型大会 2022 研讨会报告：水生动物营养与参考膳食开发。

Zebrafish Pub Date : 2023-12-01 DOI: 10.1089/zeb.2023.0079

Thomas J Sharpton, Yuan Lu, Michael L Kent, Stephen A Watts, Zoltan M Varga

{"title":"Tenth Aquatic Models of Human Disease Conference 2022 Workshop Report: Aquatics Nutrition and Reference Diet Development.","authors":"Thomas J Sharpton, Yuan Lu, Michael L Kent, Stephen A Watts, Zoltan M Varga","doi":"10.1089/zeb.2023.0079","DOIUrl":"10.1089/zeb.2023.0079","url":null,"abstract":"Progress in biomedical research requires rigorous studies and reproducible outcomes. However, despite recent achievements, standard reference diets (SRDs) for aquatic model organisms, vital for supporting scientific rigor and reproducibility, are yet to be adopted. At this workshop, we presented findings from a 7-month diet test study, tightly coordinated and conducted across three aquatic research facilities: Zebrafish International Resource Center (ZIRC), Kent and Sharpton laboratories (Oregon State University), and Xiphophorus Genetic Stock Center (XGSC, Texas State University). We compared the impact of two commercial diets and a suggested zebrafish SRD on general fish husbandry, microbiome composition, and health in three fish species (zebrafish, Xiphophorus, and Medaka), and three zebrafish wild-type strains. We reported outcomes, gathered community feedback, and addressed the aquatic research community's need for SRD development. Discussions underscored the influence of diet on aquatic research variability, emphasizing the need for SRDs to control cross-experiment and cross-laboratory reproducibility. Species-specific reference diets are essential for model organism health and consistent research outcomes.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"20 6","pages":"243-249"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10733753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138815102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Successful Rearing of Nutritionally Supplemented Rotifers (Brachionus plicatilis) at Reduced Salinity for Zebrafish (Danio rerio) Polyculture. 在降低盐度条件下成功饲养斑马鱼（Danio rerio）的营养补充轮虫（Brachionus plicatilis）。

Zebrafish Pub Date : 2023-12-01 DOI: 10.1089/zeb.2023.0027

Kathleen G L Ma, Christine Lieggi, Kvin Lertpiriyapong, Adedeji A Afolalu, Elyn R Riedel, Neil S Lipman

{"title":"Successful Rearing of Nutritionally Supplemented Rotifers (Brachionus plicatilis) at Reduced Salinity for Zebrafish (Danio rerio) Polyculture.","authors":"Kathleen G L Ma, Christine Lieggi, Kvin Lertpiriyapong, Adedeji A Afolalu, Elyn R Riedel, Neil S Lipman","doi":"10.1089/zeb.2023.0027","DOIUrl":"10.1089/zeb.2023.0027","url":null,"abstract":"Rotifers, Brachionus plicatilis, are a valuable first exogenous feed for zebrafish because they can provide continuous nutrition for growing zebrafish larvae when used in a rotifer-zebrafish polyculture. Typically cultured at high salinities (>10 ppt), B. plicatilis are temporarily immobilized when moved to lower salinities (5 ppt) used for polycultures, decreasing their accessibility and attractiveness to the larvae. The nutritional value of rotifers varies based on their diet, typically live algae, which has limited nutritional value and may pose biosecurity risks. After confirming that rotifers consume and can reproduce when fed an irradiated, processed larval fish diet (PD), they were reared at 5 or 15 ppt, and fed various combinations of an algae mix and/or PD. Population densities and percentages of egg-bearing rotifers were quantified daily until the population density plateaued, and then their nutritional value was assessed. Results indicated that rotifers thrived at both salinities. Those fed PD were successfully maintained at >500 rotifers per mL and contained a greater ω-6/ω-3 fatty acid ratio. Our findings indicate that enriching rotifers with PD raised at 5 ppt can potentially eliminate rotifer immobilization in polyculture, while providing a nutritious, attractive diet for zebrafish larvae and decreasing biosecurity risks.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"20 6","pages":"250-259"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10733754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138815099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0