Zebrafish最新文献_第7页

Acknowledgment of Reviewers 2023. 鸣谢 2023 年审稿人。

Zebrafish Pub Date : 2024-02-01 Epub Date: 2023-12-26 DOI: 10.1089/zeb.2023.29011.ack

引用次数: 0

Correction to: In Vitro Storage of Functional Sperm at Room Temperature in Zebrafish and Medaka Zebrafish 2023;20(6):229-235; doi: 10.1089/zeb.2023.0054. 更正：斑马鱼和青鳉功能精子的室温体外贮存斑马鱼 2023; 20(6):229-235; doi: 10.1089/zeb.2023.0054.

Zebrafish Pub Date : 2024-02-01 DOI: 10.1089/zeb.2023.0054.correx

引用次数: 0

Chemical and Visual Cues as Modulators of the Stress Response to Social Isolation in the Marine Medaka, Oryzias melastigma. 化学和视觉线索是海鳉社会隔离应激反应的调节剂

Zebrafish Pub Date : 2024-02-01 DOI: 10.1089/zeb.2023.0046

Alexandre Lebel, Libin Zhang, David Gonçalves

{"title":"Chemical and Visual Cues as Modulators of the Stress Response to Social Isolation in the Marine Medaka, Oryzias melastigma.","authors":"Alexandre Lebel, Libin Zhang, David Gonçalves","doi":"10.1089/zeb.2023.0046","DOIUrl":"10.1089/zeb.2023.0046","url":null,"abstract":"The marine medaka is emerging as a potential behavioral model organism for ocean studies, namely on marine ecotoxicology. However, not much is known on the behavior of the species and behavioral assays lack standardization. This study assesses the marine medaka as a potential model for chemical communication. We investigated how short exposure to visual and chemical cues mediated the stress response to social isolation with the light/dark preference test (LDPT) and the open field test (OFT). After a 5-day isolation period, and 1 h before testing, isolated fish were randomly assigned to one of four groups: (1) placed in visual contact with conspecifics; (2) exposed to a flow of holding water from a group of conspecifics; (3) exposed to both visual and chemical cues from conspecifics; or (4) not exposed to any stimuli (controls). During the LDPT, the distance traveled and transitions between zones were more pronounced in animals exposed to the conspecific's chemical stimuli. The time spent in each area did not differ between the groups, but a clear preference for the bright area in all animals indicates robust phototaxis. During the OFT, animals exposed only to chemical cues initially traveled more than those exposed to visual or both stimuli, and displayed lower thigmotaxis. Taken together, results show that chemical cues play a significant role in exploratory behavior in this species and confirm the LDPT and OFT as suitable tests for investigating chemical communication in this species.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"21 1","pages":"15-27"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139914391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Open-Source Programmable Interface for Sensory-Motor Biotests with Zebrafish (Danio rerio). 用于斑马鱼（Danio rerio）感官-运动生物测试的开源可编程界面。

Zebrafish Pub Date : 2024-02-01 Epub Date: 2024-01-09 DOI: 10.1089/zeb.2023.0067

Yutao Bai, Jason Henry, Florian Kreuder, David Mawdsley, Jan Kaslin, Donald Wlodkowic

引用次数: 0

Hidden Diversity in Eigenmannia (Gymnotiformes: Sternopygidae) Populations from Upper Paraná and Paraguay Rivers. 来自巴拉那河上游和巴拉圭河的 Eigenmannia（钩形目：Sternopygidae）种群的隐藏多样性。

Zebrafish Pub Date : 2024-02-01 Epub Date: 2024-01-29 DOI: 10.1089/zeb.2023.0107

Raissa Caroline Mazetto, Laura Ivana Ramos, Margarida Maria de Rossi Vieira, Fernando Rogério Carvalho, Luciana Andréia Borin de Carvalho, Alessandra Valéria de Oliveira, Carlos Alexandre Fernandes, Ana Luiza de Brito Portela Castro

{"title":"Hidden Diversity in Eigenmannia (Gymnotiformes: Sternopygidae) Populations from Upper Paraná and Paraguay Rivers.","authors":"Raissa Caroline Mazetto, Laura Ivana Ramos, Margarida Maria de Rossi Vieira, Fernando Rogério Carvalho, Luciana Andréia Borin de Carvalho, Alessandra Valéria de Oliveira, Carlos Alexandre Fernandes, Ana Luiza de Brito Portela Castro","doi":"10.1089/zeb.2023.0107","DOIUrl":"10.1089/zeb.2023.0107","url":null,"abstract":"Eigenmannia is a highly diverse genus within the Sternopygidae family, comprising 30 species. Due to its complex taxonomy, molecular analyses have been crucial for species delimitation within this group. Therefore, the present study presents a genetic analysis using sequences of the mitochondrial gene cytochrome c oxidase subunit 1 (COI) in specimens previously identified through alpha taxonomy as E. correntes (with unpublished data), E. virescens, and E. trilineata, originating from various locations within the Upper Paraná and Paraguay River basins in Brazil. The molecular data confirm the taxonomic complexity of the genus, as individuals morphologically identified as E. virescens and E. trilineata shared the same haplotype (H52). Furthermore, the results of the species delimitation tests suggest that specimens morphologically identified as E. virescens belong to the species E. trilineata. In addition, samples morphologically identified as E. correntes may correspond to more than one Operational Taxonomic Units (OTUs). Furthermore, the intraspecific Kimura-2-parameter (K2P) distances within the different studied populations are significant. This study has contributed valuable information about genetic diversity in Eigenmannia, emphasizing the importance of using integrative analyses to resolve taxonomic conflicts within the group. It also supports biogeographical studies and assists in biodiversity conservation efforts.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"39-47"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139571606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Affordable and Easy-to-Construct Zebrafish Housing System for Stable Long-Term Laboratory Research. 一种经济实惠且易于建造的斑马鱼住房系统，用于稳定的长期实验室研究。

Zebrafish Pub Date : 2023-12-01 Epub Date: 2023-11-22 DOI: 10.1089/zeb.2023.0061

Brian Lipscomb, Nicholas Seymour, Jada Lewis, Danny C LeBert

{"title":"An Affordable and Easy-to-Construct Zebrafish Housing System for Stable Long-Term Laboratory Research.","authors":"Brian Lipscomb, Nicholas Seymour, Jada Lewis, Danny C LeBert","doi":"10.1089/zeb.2023.0061","DOIUrl":"10.1089/zeb.2023.0061","url":null,"abstract":"Zebrafish have become a go-to model organism for in vivo studies, in part because of their reputation as being inexpensive to rear and house. Multiple do-it-yourself designs are currently available that provide laboratories with cost-effective housing systems. Unfortunately, these designs suffer from a range of issues ranging from poor water cycling rates and fragile housing tanks to inconsistent water conditions and designs that are prohibitively expensive for smaller laboratories to construct and maintain. These issues cause many of these housing systems to fall far short of the quality of commercially available zebrafish housing facilities. In this article, we present a novel, affordable, and easy-to-construct zebrafish housing system that improves upon previously published systems. The system utilizes three-dimensional printing technology to construct adaptable zebrafish tanks allowing for the housing of zebrafish at any stage of development. In addition, the water recirculation system utilizes multiple layers of filtration and no chemical adhesives, which allows for stable, long-term, housing of zebrafish in conditions suitable for research and teaching laboratories. The build described herein has been used by our laboratory to house zebrafish for over 3 years, representing multiple generations of housed fish.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"260-270"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138447723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tenth Aquatic Models of Human Disease Conference 2022 Workshop Report: Aquatics Nutrition and Reference Diet Development. 第十届人类疾病水生模型大会 2022 研讨会报告：水生动物营养与参考膳食开发。

Zebrafish Pub Date : 2023-12-01 DOI: 10.1089/zeb.2023.0079

Thomas J Sharpton, Yuan Lu, Michael L Kent, Stephen A Watts, Zoltan M Varga

{"title":"Tenth Aquatic Models of Human Disease Conference 2022 Workshop Report: Aquatics Nutrition and Reference Diet Development.","authors":"Thomas J Sharpton, Yuan Lu, Michael L Kent, Stephen A Watts, Zoltan M Varga","doi":"10.1089/zeb.2023.0079","DOIUrl":"10.1089/zeb.2023.0079","url":null,"abstract":"Progress in biomedical research requires rigorous studies and reproducible outcomes. However, despite recent achievements, standard reference diets (SRDs) for aquatic model organisms, vital for supporting scientific rigor and reproducibility, are yet to be adopted. At this workshop, we presented findings from a 7-month diet test study, tightly coordinated and conducted across three aquatic research facilities: Zebrafish International Resource Center (ZIRC), Kent and Sharpton laboratories (Oregon State University), and Xiphophorus Genetic Stock Center (XGSC, Texas State University). We compared the impact of two commercial diets and a suggested zebrafish SRD on general fish husbandry, microbiome composition, and health in three fish species (zebrafish, Xiphophorus, and Medaka), and three zebrafish wild-type strains. We reported outcomes, gathered community feedback, and addressed the aquatic research community's need for SRD development. Discussions underscored the influence of diet on aquatic research variability, emphasizing the need for SRDs to control cross-experiment and cross-laboratory reproducibility. Species-specific reference diets are essential for model organism health and consistent research outcomes.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"20 6","pages":"243-249"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10733753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138815102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Successful Rearing of Nutritionally Supplemented Rotifers (Brachionus plicatilis) at Reduced Salinity for Zebrafish (Danio rerio) Polyculture. 在降低盐度条件下成功饲养斑马鱼（Danio rerio）的营养补充轮虫（Brachionus plicatilis）。

Zebrafish Pub Date : 2023-12-01 DOI: 10.1089/zeb.2023.0027

Kathleen G L Ma, Christine Lieggi, Kvin Lertpiriyapong, Adedeji A Afolalu, Elyn R Riedel, Neil S Lipman

{"title":"Successful Rearing of Nutritionally Supplemented Rotifers (Brachionus plicatilis) at Reduced Salinity for Zebrafish (Danio rerio) Polyculture.","authors":"Kathleen G L Ma, Christine Lieggi, Kvin Lertpiriyapong, Adedeji A Afolalu, Elyn R Riedel, Neil S Lipman","doi":"10.1089/zeb.2023.0027","DOIUrl":"10.1089/zeb.2023.0027","url":null,"abstract":"Rotifers, Brachionus plicatilis, are a valuable first exogenous feed for zebrafish because they can provide continuous nutrition for growing zebrafish larvae when used in a rotifer-zebrafish polyculture. Typically cultured at high salinities (>10 ppt), B. plicatilis are temporarily immobilized when moved to lower salinities (5 ppt) used for polycultures, decreasing their accessibility and attractiveness to the larvae. The nutritional value of rotifers varies based on their diet, typically live algae, which has limited nutritional value and may pose biosecurity risks. After confirming that rotifers consume and can reproduce when fed an irradiated, processed larval fish diet (PD), they were reared at 5 or 15 ppt, and fed various combinations of an algae mix and/or PD. Population densities and percentages of egg-bearing rotifers were quantified daily until the population density plateaued, and then their nutritional value was assessed. Results indicated that rotifers thrived at both salinities. Those fed PD were successfully maintained at >500 rotifers per mL and contained a greater ω-6/ω-3 fatty acid ratio. Our findings indicate that enriching rotifers with PD raised at 5 ppt can potentially eliminate rotifer immobilization in polyculture, while providing a nutritious, attractive diet for zebrafish larvae and decreasing biosecurity risks.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"20 6","pages":"250-259"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10733754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138815099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Diversity in Natural Populations of the Near-Threatened Species Lignobrycon myersi (Characiformes, Triportheidae): Implications for Species Conservation. 近危物种木藓(特征，三足科)自然种群的遗传多样性:物种保护的意义。

Zebrafish Pub Date : 2023-12-01 Epub Date: 2023-11-27 DOI: 10.1089/zeb.2023.0036

Lisianne de Souza Oliveira, Jamille de Araújo Bitencourt, José Henrique Galdino, Iracilda Sampaio, Paulo Luiz Souza Carneiro, Paulo Roberto Antunes de Mello Affonso

{"title":"Genetic Diversity in Natural Populations of the Near-Threatened Species Lignobrycon myersi (Characiformes, Triportheidae): Implications for Species Conservation.","authors":"Lisianne de Souza Oliveira, Jamille de Araújo Bitencourt, José Henrique Galdino, Iracilda Sampaio, Paulo Luiz Souza Carneiro, Paulo Roberto Antunes de Mello Affonso","doi":"10.1089/zeb.2023.0036","DOIUrl":"10.1089/zeb.2023.0036","url":null,"abstract":"The river basins of Brazil contain a highly diverse ichthyofauna of remarkable endemism, including several threatened species. Accordingly, Lignobrycon myersi is a fish species distributed only in a few rivers from the state of Bahia, northeastern Brazil. Since this species is classified as Near Threatened and is poorly studied, efforts to understand the genetic structure of populations and putative cryptic forms should help define efficient strategies of management and conservation. Herein, the molecular identification and the population genetic diversity of specimens of L. myersi across their range (Almada, Contas, and Cachoeira river basins) were assessed using mitochondrial markers (16S rDNA and D-Loop, respectively). The inferences based on phylogenetics, genetic distance, and species delimitation methods invariably identified all samples as L. myersi. In addition, sequencing of D-loop fragments revealed significant haplotype diversity and a considerable level of population genetic structure. Despite their geographic isolation, these data suggested that populations from Almada and Contas rivers represent a single evolutionary lineage that could be managed as a whole. In contrast, the population from Cachoeira River was highly differentiated from the others and should be managed separately as a unique and endemic unit, particularly focused on the conservation of native habitats.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"271-279"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138447724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Storage of Functional Sperm at Room Temperature in Zebrafish and Medaka. 斑马鱼和鲭鱼功能精子的室温体外保存。

Zebrafish Pub Date : 2023-12-01 Epub Date: 2023-11-20 DOI: 10.1089/zeb.2023.0054

Kazumasa Takemoto, Toshiya Nishimura, Toshihiro Kawasaki, Yukiko Imai, Karine Levy, Neta Hart, Ivan Olaya, Sean M Burgess, Yaniv M Elkouby, Minoru Tanaka, Noriyoshi Sakai

{"title":"In Vitro Storage of Functional Sperm at Room Temperature in Zebrafish and Medaka.","authors":"Kazumasa Takemoto, Toshiya Nishimura, Toshihiro Kawasaki, Yukiko Imai, Karine Levy, Neta Hart, Ivan Olaya, Sean M Burgess, Yaniv M Elkouby, Minoru Tanaka, Noriyoshi Sakai","doi":"10.1089/zeb.2023.0054","DOIUrl":"10.1089/zeb.2023.0054","url":null,"abstract":"The longevity of sperm in teleost such as zebrafish and medaka is short when isolated even in saline-balanced solution at a physiological temperature. In contrast, some internal fertilizers exhibit the long-term storage of sperm, >10 months, in the female reproductive tract. This evidence implies that sperm in teleost possesses the ability to survive for a long time under suitable conditions; however, these conditions are not well understood. In this study, we show that the sperm of zebrafish can survive and maintain fertility in L-15-based storage medium supplemented with bovine serum albumin, fetal bovine serum, glucose, and lactic acid for 28 days at room temperature. The fertilized embryos developed to normal fertile adults. This storage medium was effective in medaka sperm stored for 7 days at room temperature. These results suggest that sperm from external fertilizer zebrafish and medaka has the ability to survive for at least 4 and 1 week, respectively, in the body fluid-like medium at a physiological temperature. This sperm storage method allows researchers to ship sperm by low-cost methods and to investigate key factors for motility and fertile ability in those sperm.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"229-235"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11075172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138447722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0