Zebrafish最新文献_第4页

Genotyping Zebrafish Point Mutant by Allele-Specific Blocking PCR. 通过等位基因特异性阻断 PCR 对斑马鱼点突变体进行基因分型。

Zebrafish Pub Date : 2024-08-01 Epub Date: 2024-05-29 DOI: 10.1089/zeb.2024.0138

Lih Khiang Beh, Hongyuan Shen

引用次数: 0

Zebrafish from the Alps to Sicily: The 4th Italian Zebrafish Meeting in Palermo. 从阿尔卑斯山到西西里岛的斑马鱼：在巴勒莫举行的第四届意大利斑马鱼会议。

Zebrafish Pub Date : 2024-08-01 Epub Date: 2024-07-04 DOI: 10.1089/zeb.2024.0140

Massimiliano Andreazzoli, Francesco Argenton, Cristiano Bertolucci, Dario Finazzi, Chiara Gabellini, Marina Mione, Anna Pistocchi, Massimo Santoro, Paolo Sordino, Vincenzo Cavalieri

{"title":"Zebrafish from the Alps to Sicily: The 4th Italian Zebrafish Meeting in Palermo.","authors":"Massimiliano Andreazzoli, Francesco Argenton, Cristiano Bertolucci, Dario Finazzi, Chiara Gabellini, Marina Mione, Anna Pistocchi, Massimo Santoro, Paolo Sordino, Vincenzo Cavalieri","doi":"10.1089/zeb.2024.0140","DOIUrl":"10.1089/zeb.2024.0140","url":null,"abstract":"The 4th Italian Zebrafish Meeting took place in Palermo from February 7 to 9, 2024. The primary aim of this meeting was to bring together a diverse group of principal investigators, young researchers, facility managers, commercial vendors, and others to provide an important forum for presentation and discussion of the most innovative and exciting scientific research currently ongoing in Italy using the zebrafish model. Nonetheless, the meeting program has been conceived to allow the dissemination of cutting-edge scientific research across a wide range of topics and to shed light on its future directions, without geographical boundaries. Indeed, people from various parts of the world joined the meeting, and 210 participants presented their latest work in talks and posters. Importantly, the meeting had designated time to foster open scientific exchange and informal networking opportunities among participants of all career stages, thus allowing initiation of new collaborations and strengthening of existing partnerships. The meeting was a tremendous success as testified by the highest participation ever since the first meeting of the series in 2017, coupled with the highly positive satisfaction rating expressed by the attendants. The full program and detailed information about the meeting can be found on the dedicated website at https://itazebrafishmeeting.wixsite.com/izm2024.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"275-278"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141500111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fish Playpens: Method for Raising Individual Juvenile Zebrafish on a Recirculating System for Studies Requiring Repeated Measures. 鱼类游戏池：在需要重复测量的研究中使用循环系统饲养斑马鱼幼鱼的方法。

Zebrafish Pub Date : 2024-08-01 Epub Date: 2023-12-28 DOI: 10.1089/zeb.2023.0103

Tabea O C Moll, Steven A Farber

引用次数: 0

Initial Steps of XY Sex Chromosome Differentiation in the Armored Catfish Hypostomus albopunctatus (Siluriformes: Loricariidae) Revealed by Heterochromatin Accumulation. 异染色质的积累揭示了铠甲鲶（Siluriformes: Loricariidae）XY 性染色体分化的初始步骤

Zebrafish Pub Date : 2024-06-01 Epub Date: 2024-02-20 DOI: 10.1089/zeb.2023.0100

Ligia Carla Balini, Carlos Alexandre Fernandes, Ana Luiza de Brito Portela-Castro, Rafael Fernando de Melo, Cláudio Henrique Zawadzki, Luciana Andreia Borin-Carvalho

{"title":"Initial Steps of XY Sex Chromosome Differentiation in the Armored Catfish Hypostomus albopunctatus (Siluriformes: Loricariidae) Revealed by Heterochromatin Accumulation.","authors":"Ligia Carla Balini, Carlos Alexandre Fernandes, Ana Luiza de Brito Portela-Castro, Rafael Fernando de Melo, Cláudio Henrique Zawadzki, Luciana Andreia Borin-Carvalho","doi":"10.1089/zeb.2023.0100","DOIUrl":"10.1089/zeb.2023.0100","url":null,"abstract":"In fish species, heterochromatinization is one process that could trigger sex chromosome differentiation. The present article describes a nascent XX/XY sex chromosome system evidenced by heterochromatin accumulation and microsatellite (GATA)8 in Hypostomus albopunctatus from two populations of the Paraná River basin. The specimens of H. albopunctatus from the Campo and Bossi Rivers share the same karyotype. The species exhibits 74 chromosomes (8m+14sm +16st +36a, fundamental number = 112). The C-banding technique suggests male heterogamety in H. albopunctatus, where the Y-chromosome is morphologically like the X-chromosome but differs from it for having long arms that are entirely heterochromatic. Double fluorescence in situ hybridization (FISH) with 18S and 5S rDNA probes confirmed the Ag-nucleolus organizer region sites in a single pair for both populations, and minor rDNA clusters showed interpopulational variation. FISH with the microsatellite (GATA)8 probe showed a dispersed pattern in the karyotype, accumulating these sequences of sex chromosomes of both populations. FISH with microsatellite (CGC)10 probe showed interpopulational variation. The absence of differentiated sex chromosomes in H. albopunctatus is described previously, and a new variant is documented herein where XY chromosomes can be seen in an early stage of differentiation.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"265-273"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139935017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Robust Closed-Tube Method for Resolving tp53^M214K Genotypes. 解决 tp53M214K 基因型问题的可靠闭管方法

Zebrafish Pub Date : 2024-06-01 Epub Date: 2024-02-20 DOI: 10.1089/zeb.2023.0030

Katherine M Silvius, Genevieve C Kendall

引用次数: 0

Defining the Unseen: Population-Specific Markers for Astyanax mexicanus Blind Cavefish. 定义看不见的东西：墨西哥盲洞鱼种群特异性标记。

Zebrafish Pub Date : 2024-06-01 Epub Date: 2024-02-14 DOI: 10.1089/zeb.2023.0105

Aubrey E Manning, Hannah Grunwald, Rachel Moran, Roberto Rodriguez-Morales, Amanda K Powers, Suzanne McGaugh, Johanna E Kowalko

引用次数: 0

A Chemically Inducible Muscle Ablation System for the Zebrafish. 斑马鱼化学诱导肌肉消融系统

Zebrafish Pub Date : 2024-06-01 Epub Date: 2024-03-04 DOI: 10.1089/zeb.2023.0102

Eric Paulissen, Benjamin L Martin

引用次数: 0

Modified Procedure for Simple and Rapid Collection of Zebrafish Fluid Samples for Biochemical Analyses. 简单快速采集斑马鱼体液样本进行生化分析的改进程序。

Zebrafish Pub Date : 2024-06-01 Epub Date: 2024-02-20 DOI: 10.1089/zeb.2023.0101

Ana Paula Apolinário da Silva, João Xavier da Silva Neto, José Ytalo Gomes da Silva, Ana Lorena Pereira Bezerra, Bruno Bezerra da Silva, Maria Izabel Florindo Guedes

引用次数: 0

Optimization of Zebrafish Larvae 6-OHDA Exposure for Neurotoxin Induced Dopaminergic Marker Reduction. 优化斑马鱼幼体的 6-OHDA 暴露，以减少神经毒素诱导的多巴胺能标记物。

Zebrafish Pub Date : 2024-04-12 DOI: 10.1089/zeb.2023.0098

Adrian Romero, Armando Sanchez, Jocelyn D Jones, Kristel Ledesma, M. El-Halawany, Ayman K Hamouda, Brent R Bill

{"title":"Optimization of Zebrafish Larvae 6-OHDA Exposure for Neurotoxin Induced Dopaminergic Marker Reduction.","authors":"Adrian Romero, Armando Sanchez, Jocelyn D Jones, Kristel Ledesma, M. El-Halawany, Ayman K Hamouda, Brent R Bill","doi":"10.1089/zeb.2023.0098","DOIUrl":"https://doi.org/10.1089/zeb.2023.0098","url":null,"abstract":"Parkinson's disease (PD) is a neurodegenerative disorder that is clinically assessed by motor symptoms associated with the loss of midbrain dopaminergic neurons affecting the quality of life for over 8.5 million people worldwide. The neurotoxin 6-hydroxydopamine (6-OHDA) has been used to chemically induce a PD-like state in zebrafish larvae by several laboratories; however, highly variable concentration, methodology, and reagents have resulted in conflicting results suggesting a need to investigate these issues of reproducibility. We propose a protocol that addresses the differences in methodology and induces changes in 6 days postfertilization (dpf) larvae utilizing a 24-h exposure at 3 dpf with 30 μM 6-OHDA. Despite ∼50% lethality, no morphological or development differences in surviving fish are observed. Definition of our model is defined by downregulation of the expression of th1 by reverse transcriptase-quantitative polymerase chain reaction, a marker for dopaminergic neurons and a reduction in movement. Additionally, we observed a downregulation of pink1 and an upregulation of sod1 and sod2, indicators of mitochondrial dysfunction and response to reactive oxygen species, respectively.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"9 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140712071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zebrafish as Versatile Model for Assessing Animal Venoms and Toxins: Current Applications and Future Prospects. 斑马鱼作为评估动物毒液和毒素的多功能模型：当前应用与未来展望

Zebrafish Pub Date : 2024-04-12 DOI: 10.1089/zeb.2023.0088

Fajar Sofyantoro, Nur Indah Septriani, D. S. Yudha, Ega Adhi Wicaksono, D. Priyono, Wahyu Aristyaning Putri, Alfian Primahesa, Anita Restu Puji Raharjeng, Y. A. Purwestri, T. R. Nuringtyas

{"title":"Zebrafish as Versatile Model for Assessing Animal Venoms and Toxins: Current Applications and Future Prospects.","authors":"Fajar Sofyantoro, Nur Indah Septriani, D. S. Yudha, Ega Adhi Wicaksono, D. Priyono, Wahyu Aristyaning Putri, Alfian Primahesa, Anita Restu Puji Raharjeng, Y. A. Purwestri, T. R. Nuringtyas","doi":"10.1089/zeb.2023.0088","DOIUrl":"https://doi.org/10.1089/zeb.2023.0088","url":null,"abstract":"Animal venoms and toxins hold promise as sources of novel drug candidates, therapeutic agents, and biomolecules. To fully harness their potential, it is crucial to develop reliable testing methods that provide a comprehensive understanding of their effects and mechanisms of action. However, traditional rodent assays encounter difficulties in mimicking venom-induced effects in human due to the impractical venom dosage levels. The search for reliable testing methods has led to the emergence of zebrafish (Danio rerio) as a versatile model organism for evaluating animal venoms and toxins. Zebrafish possess genetic similarities to humans, rapid development, transparency, and amenability to high-throughput assays, making it ideal for assessing the effects of animal venoms and toxins. This review highlights unique attributes of zebrafish and explores their applications in studying venom- and toxin-induced effects from various species, including snakes, jellyfish, cuttlefish, anemones, spiders, and cone snails. Through zebrafish-based research, intricate physiological responses, developmental alterations, and potential therapeutic interventions induced by venoms are revealed. Novel techniques such as CRISPR/Cas9 gene editing, optogenetics, and high-throughput screening hold great promise for advancing venom research. As zebrafish-based insights converge with findings from other models, the comprehensive understanding of venom-induced effects continues to expand, guiding the development of targeted interventions and promoting both scientific knowledge and practical applications.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"126 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140708841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0