Zebrafish最新文献_第4页

Balance Between Daily Water Renewal and Nitrate Levels in a Recirculating Zebrafish Housing System. 循环斑马鱼住宅系统中每日水更新和硝酸盐水平之间的平衡。

IF 1.2

Zebrafish Pub Date : 2025-10-01 Epub Date: 2025-08-21 DOI: 10.1177/15458547251370938

Nathan Guibert, Jean-Philippe Mocho, Laure Bally-Cuif, Sébastien Bedu

引用次数: 0

Meeting Report: Zebrafish Education and Outreach Workshop at the 18th International Zebrafish Conference in Kyoto, Japan, on August 19, 2024. 会议报告：第18届国际斑马鱼会议斑马鱼教育和推广研讨会于2024年8月19日在日本京都举行。

IF 1.2

Zebrafish Pub Date : 2025-10-01 Epub Date: 2025-08-20 DOI: 10.1177/15458547251371863

Jennifer O Liang

引用次数: 0

Extraction of Ex Vivo Macrophages from the Renal Medulla of Zebrafish (Danio rerio). 斑马鱼肾髓质离体巨噬细胞的提取。

IF 1.2

Zebrafish Pub Date : 2025-10-01 Epub Date: 2025-09-24 DOI: 10.1177/15458547251366984

Leonel Witcoski Junior, André Guilherme Portela de Paula, Jordana Dinorá de Lima, Mariana Abrantes do Amaral, Barbara Nunes Padovani, Mariana Rodrigues Davanso, Niels Olsen Saraiva Camara, Karin Braun Prado, Tarcio Teodoro Braga

{"title":"Extraction of Ex Vivo Macrophages from the Renal Medulla of Zebrafish (Danio rerio).","authors":"Leonel Witcoski Junior, André Guilherme Portela de Paula, Jordana Dinorá de Lima, Mariana Abrantes do Amaral, Barbara Nunes Padovani, Mariana Rodrigues Davanso, Niels Olsen Saraiva Camara, Karin Braun Prado, Tarcio Teodoro Braga","doi":"10.1177/15458547251366984","DOIUrl":"10.1177/15458547251366984","url":null,"abstract":"Zebrafish (Danio rerio) is recognized as a versatile model for hematopoietic studies due to its transparency, genetic similarity to humans, and ease of manipulation. This study describes a protocol for the ex vivo extraction and differentiation of macrophages from the renal medulla of zebrafish using transgenic lines and L929 cell-conditioned medium (LCCM) as an alternative to recombinant M-CSF. Adult TgMpegmCherry zebrafish were maintained under controlled conditions. Following euthanasia, renal medullary cells were isolated, treated with antibiotics, and cultured in either LCCM or recombinant human M-CSF. Macrophage differentiation was assessed using confocal microscopy (mCherry), flow cytometry, and functional phagocytosis assays. The protocol enabled efficient differentiation of progenitor cells into macrophages, with an average of 49.1% mCherry+ cells after 7 days in LCCM, outperforming recombinant M-CSF. Differentiated cells demonstrated strong phagocytic activity, confirming macrophage functionality. This method provides an accessible approach to obtaining ex vivo zebrafish-derived macrophages, enabling immunological and hematopoietic studies and allowing for functional comparisons with traditional murine macrophage protocols.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"175-181"},"PeriodicalIF":1.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Standardized Structural Environmental Enrichment for Zebrafish Kept Research Facilities. 斑马鱼养殖研究设施的标准化结构环境富集。

IF 1.2

Zebrafish Pub Date : 2025-08-01 Epub Date: 2025-06-30 DOI: 10.1089/zeb.2025.0024

Wendela Vester, Karin Pernold, Alexander Wikström, Lars Bräutigam

引用次数: 0

A Novel Method to Make Zebrafish Mate by Adding an Membrane Progestin Receptor Agonist to the Water Without Injection. 在水中不注射添加膜孕激素受体激动剂使斑马鱼交配的新方法。

IF 1.2

Zebrafish Pub Date : 2025-08-01 Epub Date: 2025-06-11 DOI: 10.1089/zeb.2025.0020

Saokat Ahamed, Md Abul Bashar, Mohammad Maksudul Hassan, Toshinobu Tokumoto

{"title":"A Novel Method to Make Zebrafish Mate by Adding an Membrane Progestin Receptor Agonist to the Water Without Injection.","authors":"Saokat Ahamed, Md Abul Bashar, Mohammad Maksudul Hassan, Toshinobu Tokumoto","doi":"10.1089/zeb.2025.0020","DOIUrl":"10.1089/zeb.2025.0020","url":null,"abstract":"This study investigates a novel method to induce mating in zebrafish (Danio rerio) by direct water addition of membrane progestin receptor (mPR) agonists, Org OD 02-0 (Org), or a maturation-inducing steroid, 17α,20β-dihydroxy-4-pregnen-3-one (DHP). Traditional spawning induction methods using hormone injections can be invasive and stressful for fish, especially small fish such as zebrafish. A previous study showed that progestins applied externally to the water can induce oocyte maturation and ovulation in female zebrafish. Then, a recent study showed that the addition of steroids and Org to the water also induced male sexual behavior in zebrafish. Therefore, it was hypothesized that the addition of these compounds to the water could induce mating in zebrafish. To address this, we investigated the efficacy of adding these compounds to the water in the vessel with a pair of zebrafish. Female and male zebrafish pairs were exposed to varying concentrations of DHP or Org in the water, and the resulting spawning rates and rate of sexual behavior induction were recorded. Our results showed that both simple addition of DHP and Org solution successfully induced mating within 4 h. A single chemical induced both spawning in females and sexual behavior in males. In this study, we have established a simple, novel method to induce mating in zebrafish by adding mPR agonists to the water.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"136-140"},"PeriodicalIF":1.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144268323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overfeeding, Agavins, and Dietary Fat: Factors that Modulate the Zebrafish Gut Microbiota. 过度喂养，Agavins和膳食脂肪：调节斑马鱼肠道微生物群的因素。

IF 1.2

Zebrafish Pub Date : 2025-08-01 DOI: 10.1177/15458547251365846

Erick Navarro-Barrón, Crisantema Hernández-González, Raúl Llera-Herrera, Alejandra García-Gasca, Mercedes López-Pérez, Bruno Gómez-Gil

{"title":"Overfeeding, Agavins, and Dietary Fat: Factors that Modulate the Zebrafish Gut Microbiota.","authors":"Erick Navarro-Barrón, Crisantema Hernández-González, Raúl Llera-Herrera, Alejandra García-Gasca, Mercedes López-Pérez, Bruno Gómez-Gil","doi":"10.1177/15458547251365846","DOIUrl":"10.1177/15458547251365846","url":null,"abstract":"This study investigates the effects of low-fat diet overfeeding on biological parameters and gut microbiota in adult male zebrafish (Danio rerio). Zebrafish were assigned to four treatment groups: two received control low-fat diets (LF: low-fat; LFA: low-fat with 5% agavins), while the others were overfed these diets (LFO: overfed low-fat; LFAO: overfed low-fat with agavins). Results indicated that low-fat overfeeding led to weight gain and increased biometric indices indicative of overweight and obesity, alongside notable changes in gut microbiota. Agavin supplementation significantly reduced food intake (p < 0.05) and decreased body weight and fat in the overfed groups. Multivariate analyses (permutational multivariate analysis of variance and analysis of similarities) revealed that the LFAO treatment significantly influenced gut microbiota composition, as assessed through 16S ribosomal RNA gene sequencing (V3 region). Noticeable differences in bacterial composition were observed between LFA and LFAO, especially regarding the genera Aeromonas, Paraburkholderia, and an unclassified genus from the Fusobacteriaceae, which contributed to these differences. While dietary factors, specifically agavins, exerted a limited influence on the composition of gut microbiota, the feeding regimen demonstrated a considerable effect.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"22 4","pages":"141-151"},"PeriodicalIF":1.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Zebrafish Neurobehavioral Catalog-Volume 2 (New Addition). 斑马鱼神经行为目录第二卷（新增）。

IF 1.2

Zebrafish Pub Date : 2025-08-01 Epub Date: 2025-08-08 DOI: 10.1089/zeb.2025.0016

Allan V Kalueff, Murilo S de Abreu, Carla D Bonan, Konstantin A Demin, Barbara D Fontana, Courtney Hillman, Matthew O Parker, Denis B Rosemberg, Ana M Valentim, De Wet Wolmarans, Brian H Harvey, Adam Michael Stewart, Longen Yang, Jiahao Cui

{"title":"The Zebrafish Neurobehavioral Catalog-Volume 2 (New Addition).","authors":"Allan V Kalueff, Murilo S de Abreu, Carla D Bonan, Konstantin A Demin, Barbara D Fontana, Courtney Hillman, Matthew O Parker, Denis B Rosemberg, Ana M Valentim, De Wet Wolmarans, Brian H Harvey, Adam Michael Stewart, Longen Yang, Jiahao Cui","doi":"10.1089/zeb.2025.0016","DOIUrl":"10.1089/zeb.2025.0016","url":null,"abstract":"The zebrafish is no longer a shy \"beginner\" but a key model species in neuroscience and drug discovery research. Understanding zebrafish behaviors is important for developing valid models of brain disorders and probing their genetic and environmental causes. The rapid development of zebrafish-based tests and screens also necessitates formal and consistent terminology for the observed phenotypes and behavioral phenomena. To address these needs, in 2013 we compiled a comprehensive 'consensus' glossary of nearly 190 zebrafish behaviors-the Zebrafish Behavior Catalog (ZBC-1) for both larval and adult fish. However, as novel zebrafish behaviors continue to be recognized, an expansion and revision of ZBC were deemed timely. Here, we present the next additional volume of the catalog (ZBC-2), which describes over 120 novel zebrafish behavioral terms and, like the original project, represents a result of a global teamwork of multiple collaborating zebrafish laboratories. Illustrating the value of community efforts to improve data replicability and reliability, this catalog is expected to help interpret research findings, foster cross-domain and cross-species modeling, and encourage new laboratories to study zebrafish behavioral phenotypes.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"117-135"},"PeriodicalIF":1.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144805502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Starting a Shoal: First Delhi/NCR Zebrafish Researchers Meeting. 开始一个浅滩：第一次德里/NCR斑马鱼研究人员会议。

IF 1.2

Zebrafish Pub Date : 2025-08-01 Epub Date: 2025-07-24 DOI: 10.1177/15458547251358902

Hemlata, Ayushma, Sunita S Shankaran, Shilpi Minocha

{"title":"Starting a Shoal: First Delhi/NCR Zebrafish Researchers Meeting.","authors":"Hemlata, Ayushma, Sunita S Shankaran, Shilpi Minocha","doi":"10.1177/15458547251358902","DOIUrl":"10.1177/15458547251358902","url":null,"abstract":"The first Delhi/NCR Zebrafish Researchers Meeting, held at the Indian Institute of Technology Delhi (IITD) on August 22, 2024, marked a significant step toward initiating collaboration and knowledge exchange among scientists utilizing zebrafish as a model system. This gathering brought together Delhi/NCR region-specific researchers from diverse fields, including developmental biology, toxicology, regeneration, neurobiology, behavior, and disease modeling, to establish a platform for interdisciplinary interaction and scientific advancement. The event featured short talks by principal investigators (PIs), each highlighting their lab's research and the role of zebrafish in their work. Additionally, a student poster session enriched discussions by showcasing cutting-edge applications of zebrafish in fundamental and translational research. The meeting facilitated in-depth conversations on the genetic and molecular manipulability of zebrafish, emphasizing their potential to address complex biological questions. This initiative laid the foundation for a periodic networking event, with participating PIs taking turns as hosts. By uniting experts from various disciplines, the meeting reinforced the importance of collaborative research and innovative approaches, paving the way for expanding zebrafish applications and strengthening the research ecosystem in India.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"158-167"},"PeriodicalIF":1.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144719319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anxiety and Fryes: A Novel Model to Study Anxiety in Zebrafish Larvae. 焦虑与焦虑：斑马鱼幼虫焦虑研究的新模型。

Zebrafish Pub Date : 2025-06-01 Epub Date: 2025-05-15 DOI: 10.1089/zeb.2025.0002

Ana Cláudia Costa de Carvalho, Sílvio Morato, Amauri Gouveia, André Walsh-Monteiro

{"title":"Anxiety and Fryes: A Novel Model to Study Anxiety in Zebrafish Larvae.","authors":"Ana Cláudia Costa de Carvalho, Sílvio Morato, Amauri Gouveia, André Walsh-Monteiro","doi":"10.1089/zeb.2025.0002","DOIUrl":"10.1089/zeb.2025.0002","url":null,"abstract":"Over the last few decades, zebrafish have proven to be a valuable tool for biomedical research, with advantages mainly in the use of embryos and adults. The larval stage, on the contrary, is somewhat less used, generally due to a lack of protocols compared with the other stages. In this study, we propose a protocol to study anxiety-like behavior in larvae using the plus maze with ramp (PMR). In the PMR, anxious behavior is measured by the time spent on the flat arms relative to the ramped arms of the apparatus. In the first phase of the study, animals at 5, 14, and 21 days postfertilization (dpf) were exposed to the PMR at different water column heights and session times. It was observed that animals at 14 and 21 dpf were more sensitive to the PMR and exhibited obvious anxiety-like behavior compared with larvae at 5 dpf. In the second phase of the study, to assess the robustness of the type-anxious response exhibited by the larvae, animals at 5, 10, and 14 dpf treated with anxiolytic drugs were exposed to PMR. Alcohol exposure showed a bimodal effect for animals at 10 and 14 dpf, with an anxiolytic effect at intermediate doses, with more exploration of the apparatus and time in the ramp arms. Higher doses caused a reduction in locomotor activity characteristic of drunkenness. Exposure to clonazepam produced anxiolytic effects and a reduction in locomotor activity at the highest dose in 10 and 14 dpf animals. On the contrary, 5 dpf animals showed unexpected effects, which we believe to be related to their stage of development. The results indicate that the PMR is an effective tool for assessing anxiety-like behavior in zebrafish larvae and is sensitive to anxiolytic drugs. Furthermore, anxiety-like behavior appears to vary during the early stages of larval development.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"86-98"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How to Dissect Zebrafish: A Standardized Sampling Protocol for Histological and Molecular Studies in Adult Zebrafish. 如何解剖斑马鱼：成年斑马鱼组织学和分子研究的标准化采样协议。

Zebrafish Pub Date : 2025-06-01 Epub Date: 2025-04-26 DOI: 10.1089/zeb.2024.0184

Martín Pérez Díaz, Ana Manuela de Azevedo Gomes, María Isabel Quiroga Berdeal, Roberto Bermúdez Pose

{"title":"How to Dissect Zebrafish: A Standardized Sampling Protocol for Histological and Molecular Studies in Adult Zebrafish.","authors":"Martín Pérez Díaz, Ana Manuela de Azevedo Gomes, María Isabel Quiroga Berdeal, Roberto Bermúdez Pose","doi":"10.1089/zeb.2024.0184","DOIUrl":"10.1089/zeb.2024.0184","url":null,"abstract":"The zebrafish (Danio rerio) has become an essential model in fields such as developmental biology, toxicology, genetics, and regenerative medicine due to its low cost, small size, transparent embryos, and genetic similarity to humans. Nowadays, this model is increasingly recognized as a valuable tool in other fields, including veterinary medicine and animal production research, particularly aquaculture, due to its unique characteristics that make it suitable for studying economically significant diseases affecting production species. However, unlike established models such as mice, zebrafish lack standardized protocols for housing, feeding, anesthesia, and sample collection, which affects study reproducibility. In addition, it is a common practice to use whole zebrafish larvae or juveniles for metagenomic studies rather than analyzing individual organs, despite the fact that gene expression can vary between organs. This approach complicates the attribution of findings to specific biological processes. To address this, various authors proposed protocols for sample collection in larvae, juveniles, and adult zebrafish; however, comprehensive studies encompassing nearly all fish organs are scarce. Understanding zebrafish anatomy and the technical requirements of the study is essential for accurate sample collection. Some challenges present during zebrafish dissection include the small size of the fish, the fragility of their organs, and the faster onset of autolysis and heterolysis after the death of the animal. Using magnifying lenses, microdissection tools, and conducting dissections on cold surfaces can help mitigate these issues. This article aims to improve sample collection for histopathological and genetic studies in adult zebrafish by establishing a comprehensive, organized, and systematic dissection protocol that accounts for the anatomical specifics of this experimental model.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"76-85"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0