Zebrafish最新文献_第3页

Effects of Oregano (Origanum vulgare) Essential Oil Supplementation on Growth Performance of Zebrafish (Danio rerio) Fed a High-Inclusion Soybean Meal Diet. 补充牛至（Origanum vulgare）精油对以高含豆粕为饲料的斑马鱼（Danio rerio）生长性能的影响

Zebrafish Pub Date : 2024-10-01 Epub Date: 2024-07-23 DOI: 10.1089/zeb.2024.0139

Aubrey Dissinger, Jacob Bowman, Giovanni S Molinari, Karolina Kwasek

{"title":"Effects of Oregano (Origanum vulgare) Essential Oil Supplementation on Growth Performance of Zebrafish (Danio rerio) Fed a High-Inclusion Soybean Meal Diet.","authors":"Aubrey Dissinger, Jacob Bowman, Giovanni S Molinari, Karolina Kwasek","doi":"10.1089/zeb.2024.0139","DOIUrl":"10.1089/zeb.2024.0139","url":null,"abstract":"Soybean meal (SBM) has become a common dietary replacement for fish meal (FM) in aquafeed. However, at high inclusions, SBM has been shown to have negative impacts presenting as reduced feed intake and intestinal inflammation. Medicinal plant extracts, namely essential oils, have been used to promote growth performance and immune response. The objective of this study was to investigate the potential therapeutic effects of oregano (Origanum vulgare) essential oil (OEO) inclusion on utilization of a high-inclusion SBM diet using zebrafish as a model. Five diets were used in this study: reference-FM-based diet, control-55.7% inclusion SBM diet, and three experimental SBM-based diets OEO1, OEO2, and OEO3 that were supplemented with 1%, 2%, or 3% of oregano oil, respectively. The FM group had overall better growth performance when compared with the other treatment groups; however, the OEO3 mean weight and feed conversion ratio were not significantly different from the FM group (p > 0.05) and were significantly improved compared with the SBM group (p < 0.05). Similarly, OEO2 total length was not significantly different from FM (p > 0.05) but significantly higher than the SBM group (p < 0.05). Expression of inflammation-related genes did not significantly differ between the OEO groups and the SBM-only group. However, the OEO2 and OEO3 groups displayed improved growth performance compared with the SBM group, suggesting that inclusion of OEO at or above 2% inclusion may help to alleviate common symptoms induced by a high-inclusion SBM diet.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"338-348"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141753770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Simple Tube Escape Assay to Test Learning and Memory in Zebrafish with Minimized Habituation. 用简单的试管逃逸试验测试斑马鱼的学习和记忆能力，同时尽量减少习惯化。

Zebrafish Pub Date : 2024-10-01 Epub Date: 2024-05-15 DOI: 10.1089/zeb.2023.0045

Dilan Wellalage Don, Tae-Yoon Kim, Bin Na Hong, Jeong-Soo Lee, Tong Ho Kang, Robert Gerlai, Cheol-Hee Kim

{"title":"A Simple Tube Escape Assay to Test Learning and Memory in Zebrafish with Minimized Habituation.","authors":"Dilan Wellalage Don, Tae-Yoon Kim, Bin Na Hong, Jeong-Soo Lee, Tong Ho Kang, Robert Gerlai, Cheol-Hee Kim","doi":"10.1089/zeb.2023.0045","DOIUrl":"10.1089/zeb.2023.0045","url":null,"abstract":"Various methods have been used in rodents to evaluate learning and memory. Although much less frequently used, the zebrafish emerges as an alternative model organism in this context. For example, it allows assessing potential behavioral deficits because of neurodevelopmental disorders or environmental neurotoxins. A variety of learning tasks have been employed in previous studies that required extensive habituation and training sessions. Here, we introduce a simpler and faster method to evaluate learning and memory of zebrafish with minimum habituation. A new apparatus, a transparent L-shaped tube, was developed in which we trained each zebrafish to swim through a long arm and measured the time to swim through this arm. We demonstrate that in this task, zebrafish could acquire both short-term (1 h) and long-term memory (4 days). We also studied learning and memory of a gene knockout (KO) zebrafish that showed social impairments related to autism. We found KO mutant zebrafish to show a quantitative impairment in habituation, learning, and memory performance compared with wild-type control fish. In conclusion, we established a novel learning apparatus and sensitive paradigm that allowed us to evaluate learning and memory of adult zebrafish that required only a brief habituation period and minimal training.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"329-337"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140924253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zebrafish Maintenance Conditions Affect Behavioral and Biochemical Biomarkers: A Possible Interfering Factor on the Research Results. 斑马鱼饲养条件影响行为和生化生物标志物：研究结果的可能干扰因素。

Zebrafish Pub Date : 2024-10-01 Epub Date: 2024-07-08 DOI: 10.1089/zeb.2024.0137

Francieli Ubirajara India Amaral, Aline Pompermaier, Paola Aparecida de Almeida, Natalia Freddo, Luciane Dallazari da Silva do Prado, Wagner Antonio Tamagno, Gustavo Reolon, André Luiz Riva Veneral, João Antônio Duarte Lampugnani, Hévilin Corrêa Dos Santos, Amanda Gorrosterrazú, Leonardo José Gil Barcellos

{"title":"Zebrafish Maintenance Conditions Affect Behavioral and Biochemical Biomarkers: A Possible Interfering Factor on the Research Results.","authors":"Francieli Ubirajara India Amaral, Aline Pompermaier, Paola Aparecida de Almeida, Natalia Freddo, Luciane Dallazari da Silva do Prado, Wagner Antonio Tamagno, Gustavo Reolon, André Luiz Riva Veneral, João Antônio Duarte Lampugnani, Hévilin Corrêa Dos Santos, Amanda Gorrosterrazú, Leonardo José Gil Barcellos","doi":"10.1089/zeb.2024.0137","DOIUrl":"10.1089/zeb.2024.0137","url":null,"abstract":"Over the years, scientific research with fish models has grown at a rapid pace, and issues such as animal welfare are becoming increasingly important in various areas of animal husbandry and experimentation. Here, we evaluated whether Danio rerio behavior is affected by long-term maintenance (75 days) in an enriched environment or a chronic stress (CS) situation. In addition, we evaluated some biochemical parameters related to redox status. We concluded that long-term maintenance of zebrafish in enriched environment might induce an anxiety-like behavior pattern when these fish are faced with an acute subsequent stressor. These anxiety results, the increased school cohesion, and the absence of oxidative damage allow us to hypothesize that the fish maintained in environmental enrichment (EE) situation is more reactive, showing a strong protective reaction to the stress. From an applicable perspective, we show that both too much stress and too little stress are not ideal for zebrafish stocks. In CS situations, fish can habituate and might not respond optimally to test conditions. In opposite, the low stress promoted by environmental enrichment also renders the fish incapable of dealing with occasional stressors optimally, because now even normal conditions appear stressful to them and may elicit fear behaviors they normally would not exhibit.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"312-319"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141560709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rosalind Franklin Society Proudly Announces the 2023 Award Recipient for Zebrafish. 罗莎琳德-富兰克林学会自豪地宣布 2023 年斑马鱼奖获得者。

Zebrafish Pub Date : 2024-10-01 DOI: 10.1089/zeb.2024.87132.rfs2023

Joan Metcalf Hedge

引用次数: 0

Integrative Taxonomy Suggests Resurrection of Species of the Astyanax bimaculatus Group (Characiformes, Characidae). 综合分类法表明，Astyanax bimaculatus 群（鳞栉目，鳞栉科）的物种将复活。

Zebrafish Pub Date : 2024-10-01 Epub Date: 2024-07-09 DOI: 10.1089/zeb.2024.0132

Mariane Gavazzoni, Flavia C Brezinski, Thiago H Pedroso, Carla S Pavanelli, Weferson J da Graça, Daniel R Blanco, Roberto L Lui, Vladimir P Margarido

{"title":"Integrative Taxonomy Suggests Resurrection of Species of the Astyanax bimaculatus Group (Characiformes, Characidae).","authors":"Mariane Gavazzoni, Flavia C Brezinski, Thiago H Pedroso, Carla S Pavanelli, Weferson J da Graça, Daniel R Blanco, Roberto L Lui, Vladimir P Margarido","doi":"10.1089/zeb.2024.0132","DOIUrl":"10.1089/zeb.2024.0132","url":null,"abstract":"Using integrative tools can be effective for species identification, especially in complex groups like Astyanax. Astyanax bimaculatus group is composed of six valid species, including A. lacustris. \"A. altiparanae\", \"A. asuncionensis\", and \"A. jacuhiensis\" are considered as junior synonyms of A. lacustris. Seeking to test the operational taxonomic unit (OTU) status of the junior synonyms of A. lacustris (\"A. altiparanae\", \"A. asuncionensis\", and \"A. jacuhiensis\"), we used analyses through mitochondrial DNA (COI and Cytb), cytogenetic markers (classical and molecular), and morphometry (\"truss network\"). Analysis of mitochondrial DNA sequences separated A. lacustris from the other synonymized species. The cytogenetic and morphometric analyses did not corroborate the synonymization and suggest that besides A. lacustris, the OTUs A. altiparanae, A. asuncionensis, and A. jacuhiensis are valid species. The analysis of different characters proposed by the integrative taxonomy used on the same individuals could provide greater reliability and minimize the underestimation of biodiversity.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"349-359"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial Fission Is Involved in Heat Resistance in Zebrafish. 线粒体分裂与斑马鱼的耐热性有关

Zebrafish Pub Date : 2024-10-01 Epub Date: 2024-07-15 DOI: 10.1089/zeb.2024.0128

Yousheng Mao, KwangHeum Hong, Li Li, In-Koo Nam, Seok-Hyung Kim, Seong-Kyu Choe

{"title":"Mitochondrial Fission Is Involved in Heat Resistance in Zebrafish.","authors":"Yousheng Mao, KwangHeum Hong, Li Li, In-Koo Nam, Seok-Hyung Kim, Seong-Kyu Choe","doi":"10.1089/zeb.2024.0128","DOIUrl":"10.1089/zeb.2024.0128","url":null,"abstract":"Global warming and extreme weather events pose a significant threat to global biodiversity, with rising water temperatures exerting a profound influence on fish conservation and fishery development. In this study, we used zebrafish as a model organism to explore the impact of a heat acclimation period on their survival rates. The results demonstrated that a 2-month heat acclimation period almost completely mitigated heat stress-induced mortality in zebrafish. Subsequent analysis of the surviving zebrafish revealed a predominance of hepatic mitochondria in a fission state. Remarkably, a short-term fasting regimen, which induced hepatic mitochondrial fission, mirrored the outcomes of the protective effect of heat acclimation and augmented animal survival under heat stress. Conversely, treatment with a mitochondrial fission inhibitor within the fasting group attenuated the elevated survival rate. Furthermore, zebrafish embryos subjected to brief heat acclimation also exhibited increased heat resistance, a trait diminished by a chemical intervention inhibiting mitochondrial fission. This suggests a shared mechanism for heat resistance between embryos and adult zebrafish. These findings underscore the potential use of inducing mitochondrial fission to enhance heat resistance in zebrafish, offering promise for fish biodiversity conservation in the face of global warming.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"320-328"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heritable CRISPR Mutagenesis of Essential Maternal Effect Genes as a Simple Tool for Sustained Population Suppression of Invasive Species in a Zebrafish Model. 在斑马鱼模型中用可遗传的 CRISPR 基因突变法对重要的母体效应基因进行突变，以此作为持续抑制入侵物种种群的简单工具。

Zebrafish Pub Date : 2024-08-01 Epub Date: 2024-03-20 DOI: 10.1089/zeb.2023.0108

Christopher J Krueger, Zhifei Dai, Cheng Zhu, Bo Zhang

引用次数: 0

Integrating Genetic and Cytogenetic Data: A Diversity Study of Astyanax and Psalidodon (Characidae) Species from the Paraná River Basin. 整合遗传学和细胞遗传学数据：巴拉那河流域 Astyanax 和 Psalidodon (Characidae) 鱼种的多样性研究。

Zebrafish Pub Date : 2024-08-01 Epub Date: 2024-05-30 DOI: 10.1089/zeb.2024.0134

Isabelle Pereira Mari-Ribeiro, Bárbara Scorsim, Leandro Ranucci, Luciana Andréia Borin-Carvalho, Alessandra Valéria de Oliveira, Ana Luiza de Brito Portela-Castro

{"title":"Integrating Genetic and Cytogenetic Data: A Diversity Study of Astyanax and Psalidodon (Characidae) Species from the Paraná River Basin.","authors":"Isabelle Pereira Mari-Ribeiro, Bárbara Scorsim, Leandro Ranucci, Luciana Andréia Borin-Carvalho, Alessandra Valéria de Oliveira, Ana Luiza de Brito Portela-Castro","doi":"10.1089/zeb.2024.0134","DOIUrl":"10.1089/zeb.2024.0134","url":null,"abstract":"Astyanax is one of the most specious fish groups in the Neotropical region, with many cryptic species, which represents a challenge for correct identification through traditional taxonomic methods. Psalidodon is a recently resurrected genus group of species previously belonging to Astyanax, specifically those with extensive chromosomal variation of the A. scabripinnis and fasciatus complexes. In the present study, the mitochondrial genes cytochrome c oxidase subunit 1 (COI), mitochondrial ATP synthase 6 and 8 (ATPase 6/8), and NADH dehydrogenase subunit 2 (ND2) were used in conjunction with chromosomal data to characterize molecularly and cytogenetically populations of Astyanax and Psalidodon from rivers and streams of the Ivaí River Basin (Paraná Basin). The results demonstrated the effectiveness of the integrative use of molecular and cytogenetic techniques, with the confirmation of at least three species for the sampled sites: A. lacustris, P. paranae, and P. fasciatus, which showed inter- and intrapopulation karyotype variations. In addition, extensive haplotypic variation can be observed for these species within the Ivaí River Basin and throughout the Paraná River Basin. The data demonstrate a hidden diversity among the species analyzed, enrich the ichthyofaunistic knowledge of small rivers and streams, and contribute to future conservation projects in these areas.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"300-309"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141176902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotyping Zebrafish Point Mutant by Allele-Specific Blocking PCR. 通过等位基因特异性阻断 PCR 对斑马鱼点突变体进行基因分型。

Zebrafish Pub Date : 2024-08-01 Epub Date: 2024-05-29 DOI: 10.1089/zeb.2024.0138

Lih Khiang Beh, Hongyuan Shen

引用次数: 0

Zebrafish from the Alps to Sicily: The 4th Italian Zebrafish Meeting in Palermo. 从阿尔卑斯山到西西里岛的斑马鱼：在巴勒莫举行的第四届意大利斑马鱼会议。

Zebrafish Pub Date : 2024-08-01 Epub Date: 2024-07-04 DOI: 10.1089/zeb.2024.0140

Massimiliano Andreazzoli, Francesco Argenton, Cristiano Bertolucci, Dario Finazzi, Chiara Gabellini, Marina Mione, Anna Pistocchi, Massimo Santoro, Paolo Sordino, Vincenzo Cavalieri

{"title":"Zebrafish from the Alps to Sicily: The 4th Italian Zebrafish Meeting in Palermo.","authors":"Massimiliano Andreazzoli, Francesco Argenton, Cristiano Bertolucci, Dario Finazzi, Chiara Gabellini, Marina Mione, Anna Pistocchi, Massimo Santoro, Paolo Sordino, Vincenzo Cavalieri","doi":"10.1089/zeb.2024.0140","DOIUrl":"10.1089/zeb.2024.0140","url":null,"abstract":"The 4th Italian Zebrafish Meeting took place in Palermo from February 7 to 9, 2024. The primary aim of this meeting was to bring together a diverse group of principal investigators, young researchers, facility managers, commercial vendors, and others to provide an important forum for presentation and discussion of the most innovative and exciting scientific research currently ongoing in Italy using the zebrafish model. Nonetheless, the meeting program has been conceived to allow the dissemination of cutting-edge scientific research across a wide range of topics and to shed light on its future directions, without geographical boundaries. Indeed, people from various parts of the world joined the meeting, and 210 participants presented their latest work in talks and posters. Importantly, the meeting had designated time to foster open scientific exchange and informal networking opportunities among participants of all career stages, thus allowing initiation of new collaborations and strengthening of existing partnerships. The meeting was a tremendous success as testified by the highest participation ever since the first meeting of the series in 2017, coupled with the highly positive satisfaction rating expressed by the attendants. The full program and detailed information about the meeting can be found on the dedicated website at https://itazebrafishmeeting.wixsite.com/izm2024.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"275-278"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141500111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0