Zebrafish最新文献_第3页

Mapping the Landscape: A Comprehensive Analysis of Zebrafish Research in Brazil (2020-2025). 绘制景观：对巴西斑马鱼研究的综合分析（2020-2025）。

IF 1.2

Zebrafish Pub Date : 2026-02-01 Epub Date: 2026-02-09 DOI: 10.1177/15458547261420783

Monica Lopes-Ferreira, Daniela Coelho Cunha, Darlan Gusso, Manuela Jacobina Sa Mj, Felipe Justiniano Pinto, Carla Lima

{"title":"Mapping the Landscape: A Comprehensive Analysis of Zebrafish Research in Brazil (2020-2025).","authors":"Monica Lopes-Ferreira, Daniela Coelho Cunha, Darlan Gusso, Manuela Jacobina Sa Mj, Felipe Justiniano Pinto, Carla Lima","doi":"10.1177/15458547261420783","DOIUrl":"10.1177/15458547261420783","url":null,"abstract":"Brazil has emerged as a significant contributor to the global zebrafish (Danio rerio) research community, yet a comprehensive analysis of its national output, collaboration networks, and thematic focus has been lacking. This study provides a systematic bibliometric analysis of 801 Brazilian-corresponding articles published from 2020 to 2025, representing ∼2.7% of global production. Our findings reveal a marked concentration of scientific output, with the South and Southeast regions contributing ∼65% of national publications, led by the states of Rio Grande do Sul (24.8%) and São Paulo (20.4%). Despite this geographic disparity, a robust and integrative national collaboration network connects all regions, with an average 34% of publications involving interstate co-authorship. International partnerships are substantial (30.4% of output), led by the Southeast and South regions, and feature distinct geographic profiles, including the Central-West's links with South Asia. The field is characterized by a strong applied focus, with Toxicology (34% of studies), Pharmacology (18.3%), and Neuroscience (15%) dominating the research landscape, aligning with the predominant use of the adult zebrafish model (64%). Publication is heavily concentrated in environmental and toxicology journals, with nearly half of all output published by Elsevier (47%). These results map a dynamic, collaborative, and thematically focused national research community that is resilient yet faces persistent regional inequalities. The study establishes a critical baseline for understanding the structure and drivers of Brazilian science in a globally relevant model organism.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"10-20"},"PeriodicalIF":1.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146145138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cobalt Chloride-Induced Tissue Regeneration and Wound Healing Depend on HIF-1α and VEGF-A-Mediated Neovascularization. 氯化钴诱导的组织再生和伤口愈合依赖于HIF-1α和vegf - a介导的新生血管。

IF 1.2

Zebrafish Pub Date : 2025-12-01 Epub Date: 2025-10-15 DOI: 10.1177/15458547251383496

Vivek Sagayaraj Rathinasamy, Navina Paneerselvan, Sankar Jagadeeshan, Raskin Rajagopal Erusan, Reji Manjunathan

{"title":"Cobalt Chloride-Induced Tissue Regeneration and Wound Healing Depend on HIF-1α and VEGF-A-Mediated Neovascularization.","authors":"Vivek Sagayaraj Rathinasamy, Navina Paneerselvan, Sankar Jagadeeshan, Raskin Rajagopal Erusan, Reji Manjunathan","doi":"10.1177/15458547251383496","DOIUrl":"10.1177/15458547251383496","url":null,"abstract":"The study investigates the role of cobalt chloride (CoCl2), a hypoxia-inducing agent, in promoting tissue regeneration using zebrafish as a model system. Caudal fins of adult zebrafish were amputated and transdermally exposed to 1% CoCl2. The extent of fin regeneration and the neovascularization process at the growth front were analyzed. CoCl2 exposure significantly enhanced regeneration compared with controls, with increased fin length and more prominent blood vessel sprouting and anastomosis. Molecular and proteomics analyses revealed an upregulation of angiogenic and pro-angiogenic factors, particularly Vascular Endothelial Growth Factor (VEGF). To verify the role of VEGF in CoCl2-mediated tissue regeneration, the amputated fins were exposed to inhibitors such as genistein and SU5416. These results suggest that CoCl2 promotes tissue regrowth and wound healing by stimulating angiogenesis. The findings highlight the therapeutic potential of CoCl2 in enhancing regeneration and wound repair through the HIF-1α/VEGF signaling pathway, with potential implications for treating ischemic wounds.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"208-217"},"PeriodicalIF":1.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145305083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal Dynamics of c-Fos Expression in a Zebrafish Larva Model of Pentylenetetrazole-Induced Seizures. 戊四唑诱发癫痫的斑马鱼幼体模型中c-Fos表达的时间动态。

IF 1.2

Zebrafish Pub Date : 2025-12-01 DOI: 10.1177/15458547251401472

Amit Kumar, Damanpreet Singh

{"title":"Temporal Dynamics of c-Fos Expression in a Zebrafish Larva Model of Pentylenetetrazole-Induced Seizures.","authors":"Amit Kumar, Damanpreet Singh","doi":"10.1177/15458547251401472","DOIUrl":"https://doi.org/10.1177/15458547251401472","url":null,"abstract":"The use of zebrafish (Danio rerio) larva as an experimental model has gained a lot of interest in epilepsy research due to its multiple advantages over mammalian models. The present study investigated the time-dependent expression of c-fos, an immediate early gene, and a marker of neuronal activation, following pentylenetetrazole (PTZ)-induced seizures in zebrafish larvae at 7-day post-fertilization . The larvae were exposed to 8 mM PTZ for a 15-min period, transferred to fish system water, and processed for c-Fos expression analysis at 15, 30, 45, 60, and 90 min of the start of the experiment. c-fos mRNA and c-Fos protein levels were quantified, and Pearson correlation analysis was conducted to assess their relationship. PTZ exposure induced seizure-like behavior and resulted in a dynamic temporal expression of c-Fos, with both mRNA and protein achieving peak levels at 45 min and declining by 90 min. This approach applied a fixed exposure duration and defined post-exposure time points, which allowed a more accurate temporal profiling. The observed peak expression at 45 min suggested an optimal window for evaluating c-Fos expression in the PTZ-induced seizures model of zebrafish larva. These findings provided a valuable reference for selecting experimental endpoints in zebrafish larva seizure studies and enhanced the reliability of c-Fos as a marker of neuronal activation.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"22 6","pages":"223-229"},"PeriodicalIF":1.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145688851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of an In Vitro Assay for Analysis of the Mechanism Underlying Chorion Elevation in Zebrafish. 斑马鱼绒毛膜升高机制体外分析方法的建立。

IF 1.2

Zebrafish Pub Date : 2025-12-01 DOI: 10.1177/15458547251401451

Eisei Tsutsumi, Toshinobu Tokumoto

{"title":"Establishment of an In Vitro Assay for Analysis of the Mechanism Underlying Chorion Elevation in Zebrafish.","authors":"Eisei Tsutsumi, Toshinobu Tokumoto","doi":"10.1177/15458547251401451","DOIUrl":"https://doi.org/10.1177/15458547251401451","url":null,"abstract":"Chorions, also known as egg membranes, form quickly after fertilization and play a role in subsequent embryonic development in fish. While the enzymatic hardening of the chorion due to the release of cortical alveoli (CA) components by the exocytosis of CA has been well-demonstrated, the initiation mechanism of this process has remained unresolved. Knockout lines with the prss59.1 trypsin paralog gene exhibited abnormalities in chorion elevation. Prss59.1 has been shown to be expressed on the chorion's surface. Therefore, we hypothesized that a trypsin-like enzyme expressed on the chorion could trigger chorion elevation. In this study, we attempted to improve the effectiveness of Hank's solution at preventing chorion elevation. By adjusting the concentration of the solution's contents, we developed a modified Hank's solution that can stop chorion elevation almost completely. Using this solution, we demonstrated that trypsin can induce chorion elevation. These results support our hypothesis that a trypsin-like enzyme initiates chorion elevation. This assay method can be used for the further analysis of the chorion elevation mechanism.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"22 6","pages":"218-222"},"PeriodicalIF":1.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145688882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DaniocellDesktop: for Interactive Reanalysis of Wild-Type Zebrafish Single-Cell Genomic Data. 用于野生型斑马鱼单细胞基因组数据的交互式再分析。

IF 1.2

Zebrafish Pub Date : 2025-12-01 Epub Date: 2025-09-05 DOI: 10.1177/15458547251376181

Alicia Evans, Jeffrey A Farrell

引用次数: 0

A Simple and Cost-Effective Setup to Analyze Optokinetic Response in Adult Zebrafish. 一种分析成年斑马鱼光动力学反应的简单而经济的装置。

IF 1.2

Zebrafish Pub Date : 2025-12-01 Epub Date: 2025-09-11 DOI: 10.1177/15458547251378005

Aravindhan Gunaseelan, Keerthana Ragavi Narenthiran, M S Ananthakrishna Tantry, Kirankumar Santhakumar

引用次数: 0

Nondestructive Larval Genotyping of Danio rerio for Mitochondrial and Nuclear DNA Genetics. 鳙鱼线粒体和核DNA遗传无损分型研究。

IF 1.2

Zebrafish Pub Date : 2025-12-01 Epub Date: 2025-09-22 DOI: 10.1177/15458547251379703

Kyler S Mitra, Shannon R Holmberg, Mireya Mota, Ankit Sabharwal, Stephen C Ekker

{"title":"Nondestructive Larval Genotyping of Danio rerio for Mitochondrial and Nuclear DNA Genetics.","authors":"Kyler S Mitra, Shannon R Holmberg, Mireya Mota, Ankit Sabharwal, Stephen C Ekker","doi":"10.1177/15458547251379703","DOIUrl":"10.1177/15458547251379703","url":null,"abstract":"The rapid advancement of nuclear and mitochondrial genomic editing tools has created an urgent need for efficient, nonlethal larval genotyping methods in zebrafish (Danio rerio) research. This study optimizes and validates a nondestructive proteinase K digestion method for mitochondrial and nuclear DNA genotyping while characterizing its impact on larval survival and gene expression. Using optimized protocol parameters, we demonstrate successful amplification of different mitochondrial and nuclear genetic loci with consistently high sensitivity. Molecular validation through PCR, restriction fragment length polymorphism analysis, and Sanger sequencing confirmed the specificity and reliability of the extracted DNA. The method successfully detected C-to-T base edits in the mt-tl1 gene introduced using the FusX TALE Base editor system, demonstrating its applicability to gene editing studies. Both 48-well and optimized 96-well formats were used, enabling this approach to be deployed at scale. This optimized method enables researchers to correlate genotypes with phenotypes in longitudinal studies while maintaining specimen viability, particularly valuable for investigating early-onset mitochondrial diseases, and utilizes standard laboratory equipment and reagents, facilitating widespread adoption in zebrafish research while adhering to ethical principles in reducing animal mortality.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"197-207"},"PeriodicalIF":1.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13671266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Variation of Spatial and Temporal Frequencies of the Moving Field Visual Stimulus on Optokinetic Response of Adult Zebrafish. 运动场视觉刺激的时空频率变化对成年斑马鱼光动力反应的影响。

IF 1.2

Zebrafish Pub Date : 2025-10-01 Epub Date: 2025-08-06 DOI: 10.1177/15458547251365847

Barnini Bhattacharya, Shibsankar Roy, Bijay Bal, Shankarashis Mukherjee, Anuradha Bhat, Kuntal Ghosh

引用次数: 0

A Knock-in Zebrafish Reporter Line for Live Visualization of Endogenous Olig2 Protein Dynamics. 内源性寡聚2蛋白动态可视化的敲入斑马鱼报告线。

IF 1.2

Zebrafish Pub Date : 2025-10-01 Epub Date: 2025-09-24 DOI: 10.1177/15458547251376166

Chia-Teng Chang, Toru Kawanishi, Sandy Nandagopal, Sean G Megason, Tony Y-C Tsai

{"title":"A Knock-in Zebrafish Reporter Line for Live Visualization of Endogenous Olig2 Protein Dynamics.","authors":"Chia-Teng Chang, Toru Kawanishi, Sandy Nandagopal, Sean G Megason, Tony Y-C Tsai","doi":"10.1177/15458547251376166","DOIUrl":"10.1177/15458547251376166","url":null,"abstract":"The transcription factor oligodendrocyte transcription factor 2 (Olig2) plays a central role in specifying motor neurons and oligodendrocytes during vertebrate neural development. While transgenic reporter lines such as TgBAC(olig2:EGFP) have been instrumental in visualizing olig2 expression, they fall short in directly reporting endogenous protein levels and may not fully recapitulate native gene regulation. To address these limitations, we generated a TgKI(olig2-mNeonGreen) zebrafish line using CRISPR/Cas9-mediated knock-in at the endogenous olig2 locus. The resulting Olig2-mNeonGreen fusion protein localizes specifically to the nucleus, enabling direct live imaging and accurate quantification of Olig2-expressing cells. We confirmed that the knock-in preserves endogenous mRNA expression and protein function, and that homozygous fish develop normally. As proof of concept, modulation of Sonic Hedgehog signaling altered Olig2-mNeonGreen+ cell numbers as expected, confirming the reporter's responsiveness to known upstream inputs. This TgKI(olig2-mNeonGreen) line offers a robust tool for studying neural progenitor dynamics in vivo.","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":" ","pages":"182-188"},"PeriodicalIF":1.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13090830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Balance Between Daily Water Renewal and Nitrate Levels in a Recirculating Zebrafish Housing System. 循环斑马鱼住宅系统中每日水更新和硝酸盐水平之间的平衡。

IF 1.2

Zebrafish Pub Date : 2025-10-01 Epub Date: 2025-08-21 DOI: 10.1177/15458547251370938

Nathan Guibert, Jean-Philippe Mocho, Laure Bally-Cuif, Sébastien Bedu

引用次数: 0