Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology最新文献

{"title":"Galleria mellonella as a Simple Yet Reliable In Vivo Model for Nanotoxicology: Techniques and Applications.","authors":"Núria Blanco-Cabra, Joana Admella, Eduard Torrents","doi":"10.1002/wnan.70033","DOIUrl":"10.1002/wnan.70033","url":null,"abstract":"<p><p>Nanomaterials are a rapidly advancing tool with applications across various scientific fields. However, their interactions with living organisms have raised numerous safety concerns, making it crucial to develop reliable models to predict and evaluate associated toxicity effects. Traditional in vitro assays fail to mimic the true physiological responses of living organisms to nanomaterials, whereas murine and other in vivo models are time-consuming, costly, and ethically controversial. The greater wax moth, Galleria mellonella, has emerged as a promising in vivo model for nanotoxicology, serving as an effective bridge between in vitro and in vivo mammalian testing. This model combines simplicity and ethical viability with a human-conserved innate immune system, making it ideal for immunotoxicity testing. While it cannot fully replace more complex animal models, G. mellonella represents a valuable alternative for early-stage nanotoxicology screening and deserves greater recognition and integration into toxicological research. In this review, we examine all the methodologies and applications of G. mellonella in nanotoxicological studies, highlighting its potential as a reliable and ethical model for assessing nanomaterial safety. This article is categorized under: Toxicology and Regulatory Issues in Nanomedicine > Toxicology of Nanomaterials.</p>","PeriodicalId":94267,"journal":{"name":"Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology","volume":"17 5","pages":"e70033"},"PeriodicalIF":8.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145202667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex and Gender Dimensions in Hazard and Risk Assessment of Engineered Nanomaterials.","authors":"Lucija Božičević, Karolina Jagiello, Anita Sosnowska, Maciej Stepnik, Maria Dusinska, Iseult Lynch, Nikolina Peranić, Ivona Capjak, Valérie Fessard, Mihaela R Cimpan, Arno C Gutleb, Elise Runden Pran, Tomasz Puzyn, Ivana Vinković Vrček","doi":"10.1002/wnan.70034","DOIUrl":"10.1002/wnan.70034","url":null,"abstract":"<p><p>The knowledge on hazards and risks connected to human exposure to engineered nanomaterials (ENMs) is still very limited, despite several decades of research and regulatory efforts at the international level. In particular, sex/gender-related responses to such exposure have not been clearly articulated so far in any of the existing guidance documents or regulatory relevant opinions provided to the parties involved in the risk assessment and risk management of ENMs. We aimed to demonstrate the relevance of the sex/gender dimension for the characterization of the risks and hazards associated with ENMs by analyzing existing scientific data on sex-related differences in response to ENMs exposure. This was achieved by performing an extensive review of in vivo mammalian toxicity studies published in PubMed and Web of Science databases. Further analysis was performed only for data reported in publications that satisfied scientific quality criteria assessed using the GUIDEnano approach. Finally, we demonstrated the importance of the sex/gender dimension for safety testing of ENMs in the future and provided recommendations on how to include the sex/gender dimension in toxicity testing of ENMs to ensure precise, transparent, and reliable conclusions in the process of hazard and risk assessments. This article is categorized under: Toxicology and Regulatory Issues in Nanomedicine > Toxicology of Nanomaterials Toxicology and Regulatory Issues in Nanomedicine > Regulatory and Policy Issues in Nanomedicine.</p>","PeriodicalId":94267,"journal":{"name":"Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology","volume":"17 5","pages":"e70034"},"PeriodicalIF":8.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145083005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in Prodrug-Loaded Nanoparticles for Enhanced Immunotherapy Efficacy.","authors":"Yajun Xu, Jincheng Du, Ya Liu, Chuwen Luo, Zhaohui Tang, Xuesi Chen","doi":"10.1002/wnan.70028","DOIUrl":"10.1002/wnan.70028","url":null,"abstract":"<p><p>Immunotherapy has emerged as an important approach for cancer treatment. However, most drugs for activating the cancer-immunity cycle have serious side effects from systemic off-target action, endangering patients' safety. Prodrug-loaded nanoparticles integrate prodrug activation strategies based on passive or active targeted delivery, which is expected to improve the enrichment of drugs in tumors, reduce the activation of drugs in normal tissues, and enhance the drugs' tumor selectivity. For example, adjuvants represented by Toll-like receptor 7/8 (TLR7/8) agonists and stimulator of interferon genes (STING) agonists are promising for the de novo priming of antitumor immunity. However, these drugs face pressing challenges in achieving tumor-specific immune activation to enhance the therapeutic effect and minimize systemic toxicity. This review systematically analyzes recent advances in these prodrug-loaded nanoparticles for precise controlled release adjuvants in tumors, focusing on TLR7/8 and STING agonists. We discuss the tumor microenvironment-driven prodrug activation, externally triggered prodrug activation, and explore the generalized design principles and mechanisms applicable to prodrug-loaded nanoparticles. Furthermore, we explore these adjuvants' current status, challenges, and future development direction in the clinical translation. This article aims to inspire the further development of these agonists. This article is categorized under: Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease.</p>","PeriodicalId":94267,"journal":{"name":"Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology","volume":"17 4","pages":"e70028"},"PeriodicalIF":8.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144823501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming Cancer Nanotechnology Data Analysis and User Experience. Part II: Providing Future Solutions Using Large Language Models.","authors":"Weina Ke, Rui He, Mark A Jensen, Marina A Dobrovolskaia","doi":"10.1002/wnan.70029","DOIUrl":"https://doi.org/10.1002/wnan.70029","url":null,"abstract":"<p><p>The advances in cancer nanotechnologies and current efforts focused on data sharing, along with the associated challenges, have been summarized in the first part of this review. Herein, we explore the potential of Large Language Models (LLMs) to enhance user experience, using the federally funded data repository caNanoLab as a case study. By training the LLM on caNanoLab data, we demonstrate its ability to provide more comprehensive search results, well-guided data entry, and a personalized, assisted search experience. We also discuss how integrating LLMs would optimize user experience, making it easier for researchers to navigate complex data and find relevant information. Our findings suggest that LLMs have significant potential to transform cancer nanotechnology data analysis and user experience, potentially paving the way for more efficient and effective advancements in cancer research. This article is categorized under: Nanotechnology Approaches to Biology > Nanoscale Systems in Biology Therapeutic Approaches and Drug Discovery > Emerging Technologies.</p>","PeriodicalId":94267,"journal":{"name":"Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology","volume":"17 4","pages":"e70029"},"PeriodicalIF":8.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12370339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging Biomimetic Drug Delivery Nanoparticles Inspired by Extracellular Vesicles.","authors":"Viswanathan Sundaram, Santosh Aryal","doi":"10.1002/wnan.70025","DOIUrl":"https://doi.org/10.1002/wnan.70025","url":null,"abstract":"<p><p>Nanoparticles (NPs) made up of cellular components such as extracellular vesicles (EVs) with a biomimetic outlook have emerged as a revolutionary approach in nanomedicine, providing significant benefits for targeted drug administration, immunotherapy, monitoring therapeutic response, and diagnostic applications. Utilizing the distinctive characteristics of natural cell membranes, membrane proteins, and cellular contents, these biomimetic NPs acquire essential biological functions from their source and biogenesis, including immune evasion, extended circulation, and target recognition, rendering them optimal candidates for therapeutic applications. This review offers a comprehensive examination of the methodologies of EVs infused with synthetic NP systems with the goal of overcoming their respective shortcomings. For instance, EVs are biogenic with cellular targeting features, but their isolation yield is limited, and their structural and colloidal stability are weak. Whereas, we have decades of experience in the mass production of highly stable synthetic NPs, they lack cellular targeting features. Therefore, the integration of these two systems as a single entity in the field of nanomedicine has gained significant attention. In this review, we emphasized the variety of EVs sources, such as erythrocytes, leukocytes, cancer cells, and stem cells, each providing unique biological benefits. Critical procedures encompassing EV's separation, coating processes, and material integration were examined while addressing the issues, including scalability, membrane stability, and preservation of functionality. Additionally, their promise in customized medicine is analyzed, highlighting their immediate medical applications. This review seeks to elucidate the existing methodologies, their constraints, and prospective advancements in the creation of EV-derived biomimetic NPs for clinical use. This article is categorized under: Nanotechnology Approaches to Biology > Nanoscale Systems in Biology Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease.</p>","PeriodicalId":94267,"journal":{"name":"Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology","volume":"17 4","pages":"e70025"},"PeriodicalIF":8.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12370395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine Learning and Artificial Intelligence in Nanomedicine.","authors":"Wei-Chun Chou, Alexa Canchola, Fan Zhang, Zhoumeng Lin","doi":"10.1002/wnan.70027","DOIUrl":"10.1002/wnan.70027","url":null,"abstract":"<p><p>Nanomedicine harnesses nanoscale materials, such as lipid, polymeric, and inorganic nanoparticles, to deliver diagnostic or therapeutic agents for cancer, infectious disease, and neurological disorders, among others. However, translating promising nanoparticle designs into clinically approved products remains a challenge. Factors such as particle size, surface chemistry, and payload interactions must be optimized, and preclinical results often fail to predict human efficacy. In recent years, artificial intelligence (AI) and machine learning (ML) have emerged as transformative tools to address these hurdles at every stage of nanomedicine development. By rapidly screening extensive libraries and extracting structure-function relationships, AI-driven models can rationalize nanoparticle formulation, predict biodistribution, and guide optimal design. Techniques like high-throughput DNA barcoding and automated liquid handling facilitate robust, large-scale data collection, feeding into computational pipelines that expedite discovery while reducing reliance on resource-intensive trial-and-error experiments. AI-based platforms also enable improved modeling of protein corona formation, which profoundly affects nanoparticle immunogenicity and cellular uptake. Despite these advances, challenges persist in data standardization, model generalizability, and establishing a clear regulatory framework since no dedicated U.S. Food and Drug Administration (FDA) guidance addresses the intersection of AI and nanomedicine. Overcoming these limitations requires harmonized data sharing, rigorous in vivo validation, and clear ethical and regulatory guidelines. This review summarizes the rapidly evolving landscape of AI in nanomedicine, highlighting key successes in design and preclinical prediction, as well as persistent obstacles to full-scale clinical integration. By illuminating these dynamics, we aim to chart a more efficient path forward in developing next-generation nanomedicine.</p>","PeriodicalId":94267,"journal":{"name":"Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology","volume":"17 4","pages":"e70027"},"PeriodicalIF":8.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12353477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144857321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Pathogen Detection: From Traditional Methods to Nanotechnology, Biosensing and AI Integration.","authors":"Jing Xu, Muhammad Akhtar, Wei Meng, Jinming Bai, Sharon Prince, Rongqin Huang","doi":"10.1002/wnan.70022","DOIUrl":"10.1002/wnan.70022","url":null,"abstract":"<p><p>The rapid and precise detection of pathogenic microbes plays a critical role in the successful diagnosis, management, and containment of infectious diseases. In this review article, we systematically examine the latest technological progress in methodologies for identifying disease-causing microorganisms. These methods are categorized into six major approaches: traditional culture-based techniques, immunological assays, molecular biological methods, metabolic profiling, spectroscopic technologies, and emerging nanotechnology-driven innovations. Moreover, this review offers a comparative assessment of these techniques, shedding light on their underlying mechanisms, strengths, weaknesses, and practical applications in real-world scenarios. This review also discusses the integration of artificial intelligence (AI)-based techniques, paving the way for next-generation pathogen surveillance. In light of the preceding analysis, this review highlights cutting-edge technological developments that could facilitate more efficient, precise, and widely available methods for identifying pathogenic microorganisms. This article is categorized under: Diagnostic Tools > Biosensing Diagnostic Tools > In Vitro Nanoparticle-Based Sensing.</p>","PeriodicalId":94267,"journal":{"name":"Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology","volume":"17 4","pages":"e70022"},"PeriodicalIF":8.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144818880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-Agents for NIR-II Photoacoustic Imaging.","authors":"Qian Wang, Shuxin Lyu, Xiaoyu Zhang, Ruiping Zhang","doi":"10.1002/wnan.70026","DOIUrl":"10.1002/wnan.70026","url":null,"abstract":"<p><p>Photoacoustic imaging (PAI), an intriguing imaging technology, is increasingly being utilized in both basic and clinical medical research. PAI in the second near-infrared window (NIR-II) offers a series of notable advantages, enhanced depth penetration, diminished tissue autofluorescence, elevated maximum allowable exposure, and reduced background noise, which are not available in the first near-infrared window (NIR-I). These attributes underscore the considerable potential of NIR-II window for PAI in the realm of biomedical applications. This review centers on elucidating the significance of the NIR-II window for PAI, presenting an overview of NIR-II PAI contrast agents, and spotlighting the latest advancements in NIR-II PAI with an emphasis on their biomedical uses. Furthermore, prospective problems and opportunities for NIR-II PA contrast agents are highlighted. This article is categorized under: Diagnostic Tools > In Vitro Nanoparticle-Based Sensing.</p>","PeriodicalId":94267,"journal":{"name":"Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology","volume":"17 4","pages":"e70026"},"PeriodicalIF":8.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144839619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming Cancer Nanotechnology Data Analysis and User Experience. Part I: Current Challenges and Solutions Provided by caNanoLab.","authors":"Weina Ke, Rui He, Mark A Jensen, Marina A Dobrovolskaia","doi":"10.1002/wnan.70030","DOIUrl":"10.1002/wnan.70030","url":null,"abstract":"<p><p>Cancer nanotechnologies have the potential to revolutionize cancer diagnosis and treatment; however, their complexity poses challenges to data analysis and knowledge sharing. caNanoLab, a dedicated cancer nanotechnology data-sharing portal, has emerged as a valuable resource for researchers in this field. However, to fully utilize the wealth of data available in caNanoLab, there is a need for real-time descriptive statistical presentation and an optimized user experience. Herein, we provide an overview of cancer nanotechnologies and federally funded efforts to create data repositories, aiming to improve information flow and data sharing among researchers in the cancer nanotechnology field. We use caNanoLab as a case study to analyze the challenges in this area and highlight how caNanoLab addresses them. We also identify gaps and explore the potential of Large Language Models (LLMs) to improve user experience. A more detailed analysis of LLM and their applications to caNanoLab is provided in the second part of this review. This article is categorized under: Therapeutic Approaches and Drug Discovery > Emerging Technologies.</p>","PeriodicalId":94267,"journal":{"name":"Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology","volume":"17 4","pages":"e70030"},"PeriodicalIF":8.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12361717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered Extracellular Vesicles in Arthritic Diseases: Therapeutic Applications & Challenges.","authors":"Seif Ehab, Ola A Gaser, Atif Abdulwahab A Oyouni, Nader Kameli, Faisal Alzahrani, Ahmed Abdal Dayem","doi":"10.1002/wnan.70031","DOIUrl":"10.1002/wnan.70031","url":null,"abstract":"<p><p>Arthritic diseases are a significant global health challenge, highlighting the urgent need for innovative therapeutic strategies. Extracellular vesicles (EVs) have emerged as promising candidates for treating various intractable diseases. This review explores the therapeutic potential of engineered EVs in joint diseases, particularly in comparison to their parental stem cells. Recent research underscores the efficacy of EVs in treating joint diseases, especially Osteoarthritis (OA). We discuss EV engineering strategies aimed at overcoming the limitations of natural EVs. Data from preclinical trials, clinical studies, and in vitro and in vivo reports are analyzed to evaluate the effectiveness of EVs in treating joint conditions. In addition to their role in intercellular communication, EVs influence various biological processes crucial for bone remodeling, cartilage regeneration, immunomodulation, and inflammation control. EVs are rich in vital biomolecules such as proteins, microRNAs (miRNA), lipids, and nucleic acids, which enhance their therapeutic potential compared to parental stem cells. This understanding is key to developing targeted and effective engineered EVs for OA and other joint diseases. A comprehensive grasp of EV engineering and underlying mechanisms will pave the way for novel and efficient therapies for arthritic diseases and related conditions. This article is categorized under: Implantable Materials and Surgical Technologies > Nanotechnology in Tissue Repair and Replacement Nanotechnology Approaches to Biology > Cells at the Nanoscale Biology-Inspired Nanomaterials > Peptide-Based Structures.</p>","PeriodicalId":94267,"journal":{"name":"Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology","volume":"17 4","pages":"e70031"},"PeriodicalIF":8.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12365376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144884653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}