Galleria mellonella as a Simple Yet Reliable In Vivo Model for Nanotoxicology: Techniques and Applications.

IF 8.2
Núria Blanco-Cabra, Joana Admella, Eduard Torrents
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Abstract

Nanomaterials are a rapidly advancing tool with applications across various scientific fields. However, their interactions with living organisms have raised numerous safety concerns, making it crucial to develop reliable models to predict and evaluate associated toxicity effects. Traditional in vitro assays fail to mimic the true physiological responses of living organisms to nanomaterials, whereas murine and other in vivo models are time-consuming, costly, and ethically controversial. The greater wax moth, Galleria mellonella, has emerged as a promising in vivo model for nanotoxicology, serving as an effective bridge between in vitro and in vivo mammalian testing. This model combines simplicity and ethical viability with a human-conserved innate immune system, making it ideal for immunotoxicity testing. While it cannot fully replace more complex animal models, G. mellonella represents a valuable alternative for early-stage nanotoxicology screening and deserves greater recognition and integration into toxicological research. In this review, we examine all the methodologies and applications of G. mellonella in nanotoxicological studies, highlighting its potential as a reliable and ethical model for assessing nanomaterial safety. This article is categorized under: Toxicology and Regulatory Issues in Nanomedicine > Toxicology of Nanomaterials.

一种简单而可靠的体内纳米毒理学模型:技术与应用。
纳米材料是一种快速发展的工具,在各个科学领域都有应用。然而,它们与生物体的相互作用引起了许多安全问题,因此开发可靠的模型来预测和评估相关的毒性效应至关重要。传统的体外试验无法模拟生物体对纳米材料的真实生理反应,而小鼠和其他体内模型既耗时又昂贵,而且在伦理上存在争议。更大的蜡蛾(Galleria mellonella)已经成为一种很有前途的体内纳米毒理学模型,可以作为体外和体内哺乳动物试验之间的有效桥梁。该模型将简单性和伦理可行性与人类保守的先天免疫系统相结合,使其成为免疫毒性测试的理想选择。虽然它不能完全取代更复杂的动物模型,但mellonella代表了早期纳米毒理学筛选的有价值的替代方案,值得更多的认识和整合到毒理学研究中。在这篇综述中,我们研究了所有的方法和在纳米毒理学研究中的应用,强调了它作为评估纳米材料安全性的可靠和伦理模型的潜力。本文分类为:纳米药物的毒理学和监管问题;纳米材料毒理学。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
17.60
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