Virology最新文献_第4页

Synergistic inhibition of HIV-1 by Nelfinavir and Epigallocatechin Gallate: A novel nanoemulsion-based therapeutic approach. 奈非那韦和没食子儿茶素没食子酸酯协同抑制HIV-1：一种新型纳米乳剂治疗方法。

Virology Pub Date : 2025-01-03 DOI: 10.1016/j.virol.2025.110391

Shraddha Y Gaikwad, Ashwini More, Chandrabhan Seniya, Kunal Verma, Madhuri Chandane-Tak, Vijay Nema, Shobhit Kumar, Anupam Mukherjee

{"title":"Synergistic inhibition of HIV-1 by Nelfinavir and Epigallocatechin Gallate: A novel nanoemulsion-based therapeutic approach.","authors":"Shraddha Y Gaikwad, Ashwini More, Chandrabhan Seniya, Kunal Verma, Madhuri Chandane-Tak, Vijay Nema, Shobhit Kumar, Anupam Mukherjee","doi":"10.1016/j.virol.2025.110391","DOIUrl":"https://doi.org/10.1016/j.virol.2025.110391","url":null,"abstract":"The integration of nanotechnology into antiretroviral drug delivery systems presents a promising avenue to address challenges posed by long-term antiretroviral therapies (ARTs), including poor bioavailability, drug-induced toxicity, and resistance. These limitations impact the therapeutic effectiveness and quality of life for individuals living with HIV. Nanodrug delivery systems, particularly nanoemulsions, have demonstrated potential in improving drug solubility, enhancing bioavailability, and minimizing systemic toxicity. Moreover, nanodrug platforms can target viral reservoirs, potentially reducing the emergence of drug-resistant strains-a significant challenge in anti-HIV treatment. This study evaluates the biological efficacy of a rosemary oil-based nanoemulsion loaded with Nelfinavir (NFV) and Epigallocatechin Gallate (EGCG), which demonstrated HIV-1 suppression at sub-CC₅₀ concentrations across two distinct cellular systems. The synergistic interaction between NFV and EGCG was confirmed through cellular assays, enzymatic studies, and molecular interaction analysis. In vitro experiments revealed that the NE-NFV-EGCG nanoemulsion exhibited enhanced HIV-1 inhibitory activity compared to pure NFV, highlighting a promising therapeutic synergy. The findings suggest that EGCG could be a valuable adjunct in NFV-based regimens for HIV management. Molecular interaction studies further confirmed the nanoemulsion's inhibitory potential against the HIV-1 protease enzyme. This study marks a significant advancement in HIV-1 treatment by documenting, for the first time, the synergistic inhibitory activity of NFV and EGCG. The novel nanoformulation offers improved oral bioavailability, minimal side effects, and enhanced therapeutic outcomes. Future studies are needed to optimize the formulation for clinical applications, including sustained drug release and drug transport mechanisms.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110391"},"PeriodicalIF":0.0,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142960908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complex interplay: The interactions between citrus tristeza virus and its host. 复杂相互作用：柑橘tristeza病毒与其宿主之间的相互作用。

Virology Pub Date : 2025-01-02 DOI: 10.1016/j.virol.2024.110388

Maryam Khalilzadeh, Dirk Jacobus Aldrich, Hans Jacob Maree, Amit Levy

引用次数: 0

Mechanisms of HSV gene regulation during latency and reactivation. HSV基因在潜伏和再激活过程中的调控机制。

Virology Pub Date : 2025-01-01 Epub Date: 2024-11-28 DOI: 10.1016/j.virol.2024.110324

Hui Fu, Dongli Pan

引用次数: 0

Development of a monoclonal antibody specifically recognizing a linear epitope on the E1 protein of Getah virus. 一种特异性识别Getah病毒E1蛋白线性表位的单克隆抗体的研制。

Virology Pub Date : 2025-01-01 Epub Date: 2024-11-26 DOI: 10.1016/j.virol.2024.110315

Muyang Liu, Tongwei Ren, Liping Zhang, Peijie Li, Zhen Zhong, Lingshan Zhou, Yifeng Qin, Kang Ouyang, Ying Chen, Weijian Huang, Zuzhang Wei

{"title":"Development of a monoclonal antibody specifically recognizing a linear epitope on the E1 protein of Getah virus.","authors":"Muyang Liu, Tongwei Ren, Liping Zhang, Peijie Li, Zhen Zhong, Lingshan Zhou, Yifeng Qin, Kang Ouyang, Ying Chen, Weijian Huang, Zuzhang Wei","doi":"10.1016/j.virol.2024.110315","DOIUrl":"10.1016/j.virol.2024.110315","url":null,"abstract":"Getah virus (GETV) is a mosquito-borne alphavirus that can cause disease outbreaks in domesticated mammals. The E1 protein of GETV plays a crucial role in mediating the fusion of viruses and host cells. In this study, the recombinant GETV E1 protein was expressed and administered to BALB/c mice. Hybridoma cells secreting a monoclonal antibody (mAb) 7D4-2 against E1 protein were subsequently obtained using a cell fusion protocol between SP2/0 cells and splenocytes. The reactivity of mAb 7D4-2 with GETV was confirmed through Western blot analysis and indirect immunofluorescence assay (IFA). A precise B cell linear epitope, 281-DIPDTAF-287, was identified using Western blot analysis with the produced mAb 7D4-2 by constructing and expressing a series of truncated His-fused E1 proteins. Conservation analysis indicated that this epitope is highly conserved in Group III strains of GETV, but exhibits an amino acid difference (T285S) compared to Group I, Group II, and Group IV. Cross-reactivity analysis by Western blot demonstrated that the B-cell epitope containing the mutation could be recognized by mAb 7D4-2. The ability of mAb 7D4-2 to recognize the epitope carrying the T285S mutation was further confirmed using an infectious clone of GETV. This study enhances the understanding of the biological characteristics of the E1 protein and will facilitate the future development of diagnostic tests for GETV.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"602 ","pages":"110315"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid test for hepatitis B core-related antigen to identify people living with hepatitis B having high viral load in Cameroon. 在喀麦隆进行乙型肝炎核心相关抗原快速检测，以确定病毒载量高的乙型肝炎患者。

Virology Pub Date : 2025-01-01 Epub Date: 2024-11-26 DOI: 10.1016/j.virol.2024.110316

Richard Njouom, Alassane Ndiaye, Abdou Fatawou Modiyinji, Frederic Lissock, Jeanne Perpétue Vincent, Masaya Baba, Naoki Yamamoto, Atsushi Kaneko, Katsumi Aoyagi, Naofumi Hashimoto, Mari Nagai, Masato Ichikawa, Tetsuo Miura, Wataru Sugiura, Yasuhito Tanaka, Yusuke Shimakawa

引用次数: 0

Development of a Duplex-ddPCR assay for accurate quantification of pseudorabies virus through systematic optimization of amplification bias. 通过系统优化扩增偏倚，建立准确定量伪狂犬病毒的双链ddpcr检测方法。

Virology Pub Date : 2025-01-01 Epub Date: 2024-11-29 DOI: 10.1016/j.virol.2024.110311

Zihan Tian, Hao Wu, Rong Xu, Lun Yao, Wentao Li, Qigai He

引用次数: 0

Research progress on the molecular mechanism of immune escape of porcine reproductive and respiratory syndrome virus. 猪繁殖与呼吸综合征病毒免疫逃逸的分子机制研究进展。

Virology Pub Date : 2025-01-01 Epub Date: 2024-12-03 DOI: 10.1016/j.virol.2024.110298

Wenwen Hu, Deyuan Tang, Zhiyong Zeng, Bin Wang, Min Zhou, Yinming Mao, Piao Zhou, Song He

{"title":"Research progress on the molecular mechanism of immune escape of porcine reproductive and respiratory syndrome virus.","authors":"Wenwen Hu, Deyuan Tang, Zhiyong Zeng, Bin Wang, Min Zhou, Yinming Mao, Piao Zhou, Song He","doi":"10.1016/j.virol.2024.110298","DOIUrl":"10.1016/j.virol.2024.110298","url":null,"abstract":"Porcine reproductive and respiratory syndrome (PRRS), caused by the porcine reproductive and respiratory syndrome virus (PRRSV), is a severe and highly contagious disease that results in significant economic losses for the pig industry. Currently, vaccination is one of the most effective methods for controlling PRRS; however, due to the extensive genetic variation of PRRSV and the generation of homologous immunity, vaccines provide protective effects only against homologous strains and lack effective cross-protection against heterologous strains. Furthermore, PRRSV encodes a variety of proteins with immune escape functions, and the mechanisms underlying these functions are complex and not yet fully understood. This complexity presents substantial challenges to the prevention, control, and eradication of the disease. Therefore, this article reviews the various escape mechanisms of PRRSV identified in recent years, with the aim of providing insights into the pathogenic mechanisms of PRRSV and facilitating the development of safer and more effective vaccines and therapeutics.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"602 ","pages":"110298"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First identification and whole genome characterization of rotavirus C in pigs in Zambia. 赞比亚猪中轮状病毒C的首次鉴定和全基因组鉴定。

Virology Pub Date : 2024-12-31 DOI: 10.1016/j.virol.2024.110385

Hayato Harima, Yongjin Qiu, Michihito Sasaki, Joseph Ndebe, Kapila Penjaninge, Edgar Simulundu, Masahiro Kajihara, Aiko Ohnuma, Keita Matsuno, Naganori Nao, Yasuko Orba, Ayato Takada, Kanako Ishihara, William W Hall, Bernard M Hang'ombe, Hirofumi Sawa

{"title":"First identification and whole genome characterization of rotavirus C in pigs in Zambia.","authors":"Hayato Harima, Yongjin Qiu, Michihito Sasaki, Joseph Ndebe, Kapila Penjaninge, Edgar Simulundu, Masahiro Kajihara, Aiko Ohnuma, Keita Matsuno, Naganori Nao, Yasuko Orba, Ayato Takada, Kanako Ishihara, William W Hall, Bernard M Hang'ombe, Hirofumi Sawa","doi":"10.1016/j.virol.2024.110385","DOIUrl":"https://doi.org/10.1016/j.virol.2024.110385","url":null,"abstract":"Rotavirus C (RVC) causes acute gastroenteritis in neonatal piglets. Despite the clinical importance of RVC infection, the distribution and prevalence in pig populations in most African countries remains unknown. In this study, we identified RVC in Zambian pigs by metagenomic analysis. The full genome sequence of the RVC revealed two different VP4 sequences, implying that two different RVC strains (ZP18-77-c1 and ZP18-77-c2) were present in the same sample. Genetic analyses demonstrated that all segments of ZP18-77-c1 and ZP18-77-c2 showed high nucleotide sequence identities (87.7-94.5%) to known porcine RVC strains, and ZP18-77-c1 and ZP18-77-c2 strains were assigned to genotype constellations, G1-P[4]/P[14]-I13-R5-C5-M1-A7-N9-T10-E5-H1. We further screened RVC genomes among pig feces collected in Zambia (n = 147) by RT-qPCR, and 78 samples (53.1%) were positive. This study demonstrated the first full genome sequence of African RVC strains with a relatively high prevalence of RVC infection in the pig populations in Zambia.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110385"},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142934280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The functions of papillomavirus E2 proteins. 乳头瘤病毒E2蛋白的功能。

Virology Pub Date : 2024-12-31 DOI: 10.1016/j.virol.2024.110387

Iain M Morgan

引用次数: 0

Mouse models of Kaposi sarcoma-associated herpesvirus (KSHV). 卡波西肉瘤相关疱疹病毒（KSHV）小鼠模型。

Virology Pub Date : 2024-12-31 DOI: 10.1016/j.virol.2024.110384

Kyle W Shifflett, Dirk P Dittmer

引用次数: 0