First identification and whole genome characterization of rotavirus C in pigs in Zambia

IF 2.8 3区医学 Q3 VIROLOGY

Virology Pub Date : 2025-02-01 DOI:10.1016/j.virol.2024.110385

Hayato Harima , Yongjin Qiu , Michihito Sasaki , Joseph Ndebe , Kapila Penjaninge , Edgar Simulundu , Masahiro Kajihara , Aiko Ohnuma , Keita Matsuno , Naganori Nao , Yasuko Orba , Ayato Takada , Kanako Ishihara , William W. Hall , Bernard M. Hang'ombe , Hirofumi Sawa

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引用次数: 0

Abstract

Rotavirus C (RVC) causes acute gastroenteritis in neonatal piglets. Despite the clinical importance of RVC infection, the distribution and prevalence in pig populations in most African countries remains unknown. In this study, we identified RVC in Zambian pigs by metagenomic analysis. The full genome sequence of the RVC revealed two different VP4 sequences, implying that two different RVC strains (ZP18-77-c1 and ZP18-77-c2) were present in the same sample. Genetic analyses demonstrated that all segments of ZP18-77-c1 and ZP18-77-c2 showed high nucleotide sequence identities (87.7–94.5%) to known porcine RVC strains, and ZP18-77-c1 and ZP18-77-c2 strains were assigned to genotype constellations, G1-P[4]/P[14]-I13-R5-C5-M1-A7-N9-T10-E5-H1. We further screened RVC genomes among pig feces collected in Zambia (n = 147) by RT-qPCR, and 78 samples (53.1%) were positive. This study demonstrated the first full genome sequence of African RVC strains with a relatively high prevalence of RVC infection in the pig populations in Zambia.

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赞比亚猪中轮状病毒C的首次鉴定和全基因组鉴定。

轮状病毒C （RVC）引起新生儿仔猪急性胃肠炎。尽管裂谷病毒感染具有临床重要性，但在大多数非洲国家的猪群中的分布和流行情况仍然未知。在这项研究中，我们通过宏基因组分析鉴定了赞比亚猪的RVC。RVC全基因组序列显示两个不同的VP4序列，表明同一样品中存在两种不同的RVC菌株（ZP18-77-c1和ZP18-77-c2）。遗传分析表明，ZP18-77-c1和ZP18-77-c2与已知猪RVC毒株核苷酸序列同源性高（87.7-94.5%），属于G1-P[4]/P[4]-I13-R5-C5-M1-A7-N9-T10-E5-H1基因型群。利用RT-qPCR技术对在赞比亚采集的147份猪粪便进行RVC基因筛选，结果显示78份（53.1%）猪粪便RVC基因阳性。本研究证实了非洲RVC毒株的第一个全基因组序列，该毒株在赞比亚猪群中具有相对较高的RVC感染率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.