Transplantation proceedings最新文献

{"title":"Inhibiting the Translocation of HMGB1 Alleviates Renal Ischemia-Reperfusion Injury by Cutting Down Inflammatory Cascade.","authors":"Qian Wei, Jia Wang, Jiong Zhang","doi":"10.1016/j.transproceed.2025.02.050","DOIUrl":"https://doi.org/10.1016/j.transproceed.2025.02.050","url":null,"abstract":"<p><strong>Background: </strong>Ischemia and reperfusion does damage to tissues and causes the decline of organ function though the inflammatory cascade. Noteworthy, HMGB1 possess a crucial role in promoting progression of these effects in kidney. The study aimed at ascertaining the concrete mechanism of HMGB1 triggering inflammatory cascade in renal ischemia-reperfusion injury (IRI).</p><p><strong>Methods: </strong>IRI was induced in mice by clamping left renal arteries for 60 minutes followed by 24 hours of reperfusion with the removal of right kidney. The effects of HMGB1on IRI were evaluated by targeting creatinine, blood urea nitrogen, survival rates, renal morphology, and the translocation and secretion of HMGB1. In addition, the expression of Toll-like receptor 4, phosphorylated nuclear factor κB p65, nuclear factor κB p65, and inflammatory cascade molecules (interleukin [IL]-1β, and IL-6, and tumor necrosis factor-α) were carried out.</p><p><strong>Results: </strong>Our results demonstrated that antibody against HMGB1 (anti-HMGB1) can improve the survival rate; decrease the expression of creatinine, blood urea nitrogen, Toll-like receptor 4, phosphorylated nuclear factor κB p65, IL-1β, IL-6, and tumor necrosis factor-α; reduce renal pathological injury; alleviate the secretion of HMGB1; and suppress the translocation of HMGB1 from nucleus into cytoplasm in IRI. Notably, recombinant HMGB1, the agonist of HMGB1, can alleviate the noted effects of anti-HMGB1 in IRI.</p><p><strong>Conclusion: </strong>HMGB1 can aggravate renal IRI by triggering the inflammatory cascade, the mechanism of which is associated with activating the Toll-like receptor 4-nuclear factor κB p65 signal pathway.</p>","PeriodicalId":94258,"journal":{"name":"Transplantation proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Safety Evaluation of TAE for Ruptured Hepatocellular Carcinoma and Ablation-Related Hemorrhage, and the Clinical Significance of Postoperative Liver Function Changes.","authors":"Wenfeng Gao, Qing Zhang, Liang Ma, Ning He, Yonghong Zhang, Chunwang Yuan","doi":"10.1016/j.transproceed.2025.02.043","DOIUrl":"https://doi.org/10.1016/j.transproceed.2025.02.043","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluates the safety of transarterial embolization (TAE) for treating ruptured hemorrhage in liver cancer and postablation bleeding, comparing postoperative liver function indicators such as alanine aminotransferase (ALT), total bilirubin (TBIL), and prothrombin time percentage (PT%).</p><p><strong>Methods: </strong>A retrospective analysis was conducted on liver function changes before and after surgery in 3 patient groups: ruptured hemorrhage, postablation bleeding, and a control group (routine TAE). The safety of TAE was assessed by comparing preoperative and postoperative levels of ALT, TBIL, and PT%, using ROC curve analysis to evaluate predictive values for liver injury.</p><p><strong>Results: </strong>All patients successfully underwent surgery, with a 100% hemostasis success rate. The 30-day survival rates were 90.91% for the rupture group and 100% for the ablation group, while 1-year survival rates were 45.45% and 85.19%, respectively. Significant pre- and postoperative changes in ALT and TBIL were found within groups, but no significant differences were observed compared to the control group. ROC analysis showed strong predictive values for ALT and TBIL increases regarding liver injury.</p><p><strong>Conclusion: </strong>TAE is a safe and effective treatment for ruptured hemorrhage and post-ablation bleeding in liver cancer. Postoperative liver function changes in these groups are comparable to controls. Monitoring ALT and TBIL is crucial for early detection of liver injury, informing timely interventions. These findings enhance TAE protocols and liver protection strategies, improving outcomes for liver cancer patients.</p>","PeriodicalId":94258,"journal":{"name":"Transplantation proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Plasmapheresis Versus Plasmapheresis Combined With Continuous Renal Replacement Therapy in Adult Liver Failure: A Retrospective Observational Study.","authors":"Ilhan Ocak, Mustafa Colak, Bilge Nur Bilici","doi":"10.1016/j.transproceed.2025.02.051","DOIUrl":"https://doi.org/10.1016/j.transproceed.2025.02.051","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Liver failure constitutes a critical medical condition marked by the rapid decline in hepatic functions. Novel therapeutic approaches, including therapeutic plasma exchange (TPE) and continuous venovenous hemodiafiltration (CVVHDF), have emerged as promising modalities for mitigating the effects of this condition by facilitating detoxification and enhancing liver function. The efficacy of these interventions, whether administered individually or in combination, is a prominent area of investigation in the management of liver failure among adult populations. This study aims to evaluate the role and effectiveness of TPE, both as a standalone treatment and in conjunction with CVVHDF, in the management of liver failure in adult patients.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;This retrospective study was conducted in a Liver Transplant Intensive Care Unit (LTICU), focusing on the medical records of adult patients aged 35 to 62 years. The patient cohort consisted of individuals admitted between January 1, 2021, and June 1, 2024, due to acute liver failure or acute-on-chronic liver failure. The analysis specifically included patients who underwent therapeutic plasma exchange (TPE) or those who received continuous renal replacement therapy in conjunction with TPE. For the statistical analysis, a P-value of less than .05 was deemed indicative of statistical significance. The study encompassed a total of 47 patients with liver failure, comprising 23 males and 24 females. Among these patients, 25 (53.2%) received only TPE, while 22 (46.8%) were treated with a combination of TPE and continuous venovenous hemodiafiltration (CVVHDF).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;In the cohort of patients who received only therapeutic plasma exchange (TPE), the median International Normalized Ratio (INR) improved significantly, decreasing from 2 (1.6-2.6) to 1.3 (1.1-1.7). Similarly, alanine aminotransferase levels reduced from 351 (66-1482) to 166 (71-367), while aspartate aminotransferase levels decreased from 259 (132-1392) to 86 (35-160). In the group receiving a combination of TPE and continuous venovenous hemodiafiltration (CVVHDF), notable reductions were also observed: INR decreased from 3 (2.4-4.7) to 1.5 (1.3-2.4), alanine aminotransferase levels dropped from 691 (59-2397) to 162 (70-1060), and aspartate aminotransferase levels fell from 916 (134-1828) to 69 (45-503). These changes were statistically significant, with P-values of less than .05 for each parameter in both treatment groups. Overall, 21 patients achieved survival without requiring a liver transplant, while 7 patients underwent liver transplantation, resulting in a transplant-free survival rate of 44.7%.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;The findings from our study on the management of liver failure in adults demonstrate that both therapeutic plasma exchange (TPE) administered alone and in conjunction with continuous venovenous hemodiafiltration (CVVHDF) are effective treatment","PeriodicalId":94258,"journal":{"name":"Transplantation proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Treatment Outcomes and Prognostic Factors in Early T-Precursor Versus Non-ETP Acute Lymphoblastic Leukemia Following Allogeneic Hematopoietic Stem Cell Transplantation in Adolescents and Young Adults.","authors":"Chengsen Cai, Huizhu Kang, Peng Ke, Mengjie Cai, Ming Gao, Chengyuan Gu, Ruju Wang, Jiaqian Qi, Depei Wu, Yue Han","doi":"10.1016/j.transproceed.2025.02.029","DOIUrl":"https://doi.org/10.1016/j.transproceed.2025.02.029","url":null,"abstract":"<p><p>This study evaluates the outcomes and risk factors associated with allogeneic hematopoietic stem cell transplantation (allo-HSCT) in adolescents and young adults (AYA) diagnosed with early T-precursor (ETP) acute lymphoblastic leukemia (ALL) compared to non-ETP ALL.We retrospectively analyzed 178 AYA patients with T-ALL who were treated between September 2014 and December 2022. Out of 178 patients, 128 underwent allo-HSCT, with a median age of 25 years (range: 18-35). Among them, 49 were in the ETP group and 79 in the non-ETP group. Neutrophil and platelet engraftment times were comparable between groups. 3-year OS rates were 67.3% for ETP and 65.8% for non-ETP patients; 3-year LFS rates were 65.3% and 65.8%, respectively, showing no significant difference. Cumulative incidence of relapse (22.1% vs. 21.6%) and non-relapse mortality rates (17.8% vs. 13.7%) were similar. The incidence of acute and chronic GVHD, along with infections, showed no significant differences. Multivariable analysis revealed first complete remission before transplantation as a positive predictor for OS and LFS in non-ETP patients. In ETP patients, a white blood cell count below 30 × 10⁹/L predicted better LFS outcomes. A hemoglobin level below 120 g/L was independently associated with improved OS in non-ETP patients. Allo-HSCT offers effective improvement in prognosis for ETP-ALL, with survival outcomes comparable to non-ETP ALL patients.</p>","PeriodicalId":94258,"journal":{"name":"Transplantation proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A 13-Year Nationwide Analysis of Nocardia and Actinomyces Infection Outcomes in Liver Transplant Recipients.","authors":"Andrej M Sodoma, James R Pellegrini, Rezwan F Munshi, Samuel Greenberg, Sonika Rathi, Tulika Saggar, Atul Sinha, Jiten Desai, Paul Mustacchia","doi":"10.1016/j.transproceed.2025.02.037","DOIUrl":"https://doi.org/10.1016/j.transproceed.2025.02.037","url":null,"abstract":"<p><p>Liver transplant (LT) recipients have a profound susceptibility to infections. Although Nocardia and Actinomyces (NAs) are well-known bacteria that typically affect immunosuppressed patients, a scarcity of research exists on the effects of LT with NA infections. Our study aims to evaluate the outcomes associated with NA infections in patients with LT. Patients were selected from the National Inpatient Sample (NIS) from 2008 through 2020. International Classification of Disease revision 9 (ICD-9) and ICD revision 10 (ICD-10) codes. Patients admitted with a history of LT were subdivided into those who were and were not diagnosed with an NA infection. Records were weighted using the NIS algorithm. Primary outcomes were all-cause hospital mortality, acute kidney injury (AKI), acute myocardial infarction (AMI), shock, and a composite of these. Secondary outcomes were length of stay, total charges, cytomegalovirus (CMV), and transplant rejection. Demographics and comorbidities were compared between the groups with a weighted chi-square test. Outcomes were compared between the two groups, and adjusted odds ratios (ORs) and regression coefficients were calculated using weighted logistic or linear regression as appropriate. ORs were adjusted for age, gender, race, hospital characteristics, Charlson Comorbidity Index (CCI), median income based on zip code, weekend admission, and insurance. There were 469,141 patients with LT who were included in this study, 310 of them had NA infection (0.07%). Patients in each group were of similar age, race, and overall medical complexity (P > .05). Patients with NA infection were less likely to have a history of coronary artery disease (CAD; 4.84% vs 16.20%, P < .05), hypertension (14.53% vs 25.82%, P < .05), and obesity (1.61% vs 9.0%, P < .05) than the healthy controls. Patients with LT with NA infection were found to have higher odds of mortality (OR = 5.50, P < .001), AKI (OR = 1.9, P < .05), composite outcome (OR = 2.19, P < 0.01), and more likely to have CMV infection (OR = 6.38, P < .01). Patients with LT with NA infection stayed 13.11 days longer in the hospital (P < .01) with charges of $60,399 more (P < .01) than the healthy controls. Patients with LT who acquired an NA infection were at nearly six-fold higher odds of death and other negative outcomes. Based on previous research that has demonstrated organ transplant patients to be at high risk of infections, more vigilant care should be taken to protect patients with LT from such opportunistic infections.</p>","PeriodicalId":94258,"journal":{"name":"Transplantation proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Barriers to Kidney Transplantation in Urban Hemodialysis Centers in Pakistan: A Cross-Sectional Study.","authors":"Hasaan Salman Shafi, Syed Abdullah Ali Shah, Irfan Ahmad, Nabiha Rizvi, Salman Tahir Shafi, Omair Farooq","doi":"10.1016/j.transproceed.2025.02.048","DOIUrl":"https://doi.org/10.1016/j.transproceed.2025.02.048","url":null,"abstract":"<p><strong>Background and aims: </strong>End-stage kidney disease is a global health challenge that has greatly affected low-income and middle-income countries. While a living donor kidney transplant (LDKT) is the most optimal treatment for end-stage kidney disease, many medical and nonmedical barriers persist which hinder transplant rates. This study aims to identify the various medical, psychological, and socioeconomic barriers to LDKT among patients in urban hemodialysis centers in Pakistan.</p><p><strong>Methods: </strong>A cross-sectional study of patients undergoing hemodialysis in urban hospitals across three urban cities in Pakistan was conducted. Participants were given a structured questionnaire addressing demographics, medical history, socioeconomic status, and transplantation awareness.</p><p><strong>Results: </strong>The median age was 54 years (IQR 20), with 56.2% male participants. Barriers were identified as socioeconomic (61.4%), medical (32.6%), and psychological (6%). Significant differences in median age were observed across groups (P < .01), with socioeconomic barriers associated with younger patients (median age 49 years) compared to medical (median 63 years) and psychological (59 years) barriers. Patients with socioeconomic barriers were younger (P ≤ .01), had lower income (P = .02), and had relatively lower rates of marriage (P = .02) compared to those in other categories. Lack of donors and lack of financial resources were the most common barriers, cited by 50.2% and 50% of participants, respectively.</p><p><strong>Conclusions: </strong>Socioeconomic barriers are the leading obstacles to LDKT in Pakistan. Efforts must be made to address these barriers, such as a deceased donor program, financial aid, and improving patient knowledge about LDKT. Further studies should explore rural populations and psychological barriers in greater depth.</p>","PeriodicalId":94258,"journal":{"name":"Transplantation proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of PNI Score on Renal Prognosis and Graft Rejection After Kidney Transplantation.","authors":"Serap Yadigar, Pınar Özdemir, Erman Özdemir","doi":"10.1016/j.transproceed.2025.02.038","DOIUrl":"https://doi.org/10.1016/j.transproceed.2025.02.038","url":null,"abstract":"<p><strong>Background: </strong>Determining effective ways to make prognostic predictions after kidney transplantation of patients is essential for the management of patients. This study examines how the PNI score affects renal outcomes after kidney transplantation. The potential impact of PNI on renal function beyond the overall nutritional status was also examined.</p><p><strong>Methods: </strong>In this retrospective study, 100 kidney transplant patients were divided into three groups according to PNI scores: low (<40), intermediate (40-45) and high (>45). Demographic characteristics, clinical parameters, serum creatinine levels, estimated glomerular filtration rate (eGFR) and parenchymal thickness were evaluated. Logistic regression analysis was applied for the risk of graft rejection. Patients who were followed up for at least 6 months after kidney transplantation and had complete clinical data were included in the study. The mean follow-up period was 36 months (range: 6-60 months).</p><p><strong>Results: </strong>There was no statistically significant correlation between PNI scores and renal function (P > .05). The mean creatinine level was 1.73 ± 1.11 mg/dL in the low PNI group and 1.37 ± 0.52 mg/dL in the high PNI group. Although this difference was close to the limit of statistical significance, it was not significant (P = .083). In logistic regression analysis, no significant effect of PNI score on graft rejection was observed (OR: 1.0015, 95% CI: 0.7975-1.2576, P = .9899). However, age (OR: 0.9247, P = .0347) and serum creatinine levels (OR: 2.8396, P = .0151) significantly affected the risk of graft rejection. No significant effect of PNI score on complication rates was observed.</p><p><strong>Conclusions: </strong>This study demonstrated that PNI score alone is not a sufficient predictor of renal prognosis and graft rejection risk after kidney transplantation. Factors such as age and serum creatinine levels were found to be more predictive of the risk of graft rejection.</p>","PeriodicalId":94258,"journal":{"name":"Transplantation proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Relationship Between Health Literacy and Adherence to Immunosuppressive Therapy in Heart Transplant Patients: A Cross-Sectional Study.","authors":"Nihal Celikturk Doruker, Tugba Nur Oden, Fatma Demir Korkmaz","doi":"10.1016/j.transproceed.2025.01.008","DOIUrl":"https://doi.org/10.1016/j.transproceed.2025.01.008","url":null,"abstract":"<p><strong>Background: </strong>Nonadherence to immunosuppressive therapy after heart transplantation, driven by factors such as patient, caregiver, institutional, policy-related issues, and health literacy, leads to rejection and increased mortality. This study aimed to investigate the relationship between health literacy and adherence to immunosuppressive therapy in heart transplant patients.</p><p><strong>Methods: </strong>The sample of this cross-sectional study consisted of 57 patients who underwent heart transplantation in a university hospital in Izmir, Turkey. \"Sociodemographic and Descriptive Characteristics Form\", \"Data on Immunosuppressive Drug Therapy Form\", \"Rapid Estimate of Adult Health Literacy in Medicine (REALM) Scale\", and \"Immunosuppressive Therapy Adherence Scale (ITAS)\" were used to collect the data..</p><p><strong>Results: </strong>The median score on the REALM scale was 64.00 (IQR = 3.00, min = 18.00, max = 66.00) and 80.7% of the patients scored adequately. The median score of the immunosuppressive therapy adherence scale was 11.00 (IQR= 1.00, min= 5.00, max= 12.00) and 42.1% of the patients scored full score. There was no statistically significant relationship between the median total scores of the REALM and ITAS scales in heart transplant patients (r = 0.181, P = .178).</p><p><strong>Conclusions: </strong>In this study, the majority of heart transplant patients had adequate health literacy. It was found that less than half of these patients adhered to immunosuppressive therapy. Less than half of these patients were found to comply with immunosuppressive therapy. In this study, no relationship was found between health literacy and adherence to immunosuppressive therapy.</p>","PeriodicalId":94258,"journal":{"name":"Transplantation proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Postoperative Acid-Base Balance and Lactate Levels as Predictors of ICU Length of Stay in Liver Transplant Patients.","authors":"Reyhan Arslantas, Mustafa Kemal Arslantas","doi":"10.1016/j.transproceed.2025.02.030","DOIUrl":"https://doi.org/10.1016/j.transproceed.2025.02.030","url":null,"abstract":"<p><strong>Purpose: </strong>Liver transplantation is a life-saving procedure for patients with end-stage liver disease. The postoperative period presents significant challenges, particularly in managing acid-base balance and lactate levels, which are crucial indicators of metabolic stability and tissue perfusion. While these parameters provide valuable insights into patient recovery, their role in predicting intensive care unit (ICU) length of stay remains unclear. This study evaluates whether early postoperative acid-base balance and lactate levels can reliably predict ICU length of stay in liver transplant recipients, aiming to enhance postoperative care strategies.</p><p><strong>Methods: </strong>A retrospective observational study was conducted on 53 adult liver transplant recipients. Acid-base and lactate parameters were measured at two-time points: early (within the first 6 hours) and late (6-24 hours) postoperatively. Paired t-tests and Wilcoxon signed-rank tests were used to compare these measurements. Multiple linear regression modeling was employed to assess the impact of these parameters on ICU length of stay.</p><p><strong>Results: </strong>Significant changes were observed in FiO₂, pH, Base Excess, HCO₃, lactate, and Strong Ion Difference (SID) between early and late postoperative measurements (P < .05). However, regression analysis revealed that maximum lactate and early SID were not strong predictors of ICU length of stay (R² = 0.062). Exploratory analyses indicated that patients with elevated SID and markedly high lactate levels tended to have prolonged ICU stays.</p><p><strong>Conclusions: </strong>While postoperative acid-base balance and lactate levels are important indicators of physiological status in liver transplant recipients, they do not serve as strong independent predictors of ICU length of stay.</p>","PeriodicalId":94258,"journal":{"name":"Transplantation proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"4-Color Flow Cytometric Cytotoxicity Crossmatch Using Whole Blood Lysis.","authors":"Dong Il Won, Jeong-Hoon Lim, Jang-Hee Cho, Chan-Duck Kim, Seung Huh","doi":"10.1016/j.transproceed.2025.02.034","DOIUrl":"https://doi.org/10.1016/j.transproceed.2025.02.034","url":null,"abstract":"<p><strong>Background: </strong>The conventional lymphocyte crossmatch (XM) assay necessitates mononuclear cell isolation. In our previous work, we introduced a novel 3-laser, 4-color flow cytometric (FC) XM protocol utilizing whole blood lysis (WBL) and CD45 fluorescence-triggered acquisition to detect human leukocyte antigen (HLA) antibody binding. Building on this, we aimed to adapt these advancements for complement-dependent cytotoxicity (CDC) XM using FC (FCCDC).</p><p><strong>Methods: </strong>A total of 164 donor/recipient pairs undergoing transplantation were stratified into 2 groups based on donor-specific HLA alloantibody presence: DSA-positive (DSA+, n = 73) and DSA-negative (DSA-, n = 91). The DSA- group was further subdivided by ABO compatibility into ABO-incompatible (ABOi, n = 52) and ABO-compatible (n = 39) subgroups. Protocol optimization for the WBL FCCDC with CD45 V500-C was conducted using a FACSLyric cytometer (BD Biosciences). T and B cell indices were calculated as delta (test minus control) percentages of dead cells (Δ%DC). WBL FCCDC results were compared with those of conventional FCCDC in each group.</p><p><strong>Results: </strong>WBL FCCDC showed no significant quantitative difference from conventional FCCDC. In the DSA+ group, B cell Δ%DC values were 28.00 ± 27.14 and 19.16 ± 27.74 for WBL FCCDC and conventional FCCDC, respectively (P = .0777). No ABO antibody interference was observed in the ABOi subgroup. Qualitatively, B cell WBL FCCDC sensitivity in the DSA+ group was 69.9%, comparable to conventional FCCDC sensitivity (65.8%, P = .5078). Additionally, WBL FCCDC reduced the turnaround time by 50 minutes relative to conventional FCCDC.</p><p><strong>Conclusions: </strong>WBL FCCDC achieved performance equivalent to conventional FCCDC, similar to WBL FCXM. The absence of adverse effects from the lysis step supports its integration into XM assays. Given its simplicity and maintained sensitivity, the WBL FCCDC protocol presents a viable alternative to conventional methods in histocompatibility laboratories.</p>","PeriodicalId":94258,"journal":{"name":"Transplantation proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}