The Journal of investigative dermatology最新文献_第4页

Correlating Topographic Imaging and Raman Microscopy to Investigate Wound Re-Epithelialization. 将地形图成像和雷曼显微镜相关联，以研究伤口的再上皮化。

The Journal of investigative dermatology Pub Date : 2024-09-27 DOI: 10.1016/j.jid.2024.08.031

Pia Katharina Vestweber, Ines Ana Ederer, Ulrich Michael Rieger, Ernst H K Stelzer, Viktoria Planz, Maike Windbergs

引用次数: 0

Profiling Long Noncoding RNA in Psoriatic Skin Using Single-Cell RNA Sequencing. 利用 scRNA-seq 分析银屑病皮肤中的 lncRNA。

The Journal of investigative dermatology Pub Date : 2024-09-27 DOI: 10.1016/j.jid.2024.09.010

Rachael Bogle, Matthew T Patrick, Sutharzan Sreeskandarajan, Mehrnaz Gharaee-Kermani, Haihan Zhang, Qinmengge Li, Ruiwen Zhou, Feiyang Ma, J Michelle Kahlenberg, Olesya Plazyo, James T Elder, Allison C Billi, Johann E Gudjonsson, Lam C Tsoi

{"title":"Profiling Long Noncoding RNA in Psoriatic Skin Using Single-Cell RNA Sequencing.","authors":"Rachael Bogle, Matthew T Patrick, Sutharzan Sreeskandarajan, Mehrnaz Gharaee-Kermani, Haihan Zhang, Qinmengge Li, Ruiwen Zhou, Feiyang Ma, J Michelle Kahlenberg, Olesya Plazyo, James T Elder, Allison C Billi, Johann E Gudjonsson, Lam C Tsoi","doi":"10.1016/j.jid.2024.09.010","DOIUrl":"10.1016/j.jid.2024.09.010","url":null,"abstract":"The expressions of long noncoding RNAs (lncRNAs) and their roles in epidermal differentiation have been previously defined using bulk RNA sequencing. Despite their tissue-specific expression profiles, most lncRNAs are not well-annotated at the single-cell level. In this study, we evaluated the use of single-cell RNA sequencing to profile and characterize lncRNAs using data from 6 patients with psoriasis with paired uninvolved and lesional psoriatic skin. Despite their overall lower expression, we were able to detect >7000 skin-expressing lncRNAs and their cellular sources. Differential gene expression analysis revealed 137 differentially expressed lncRNAs in lesional psoriasis skin and identified 169 cell-type-specific lncRNAs. Keratinocytes had the highest number of differentially expressed lncRNA in psoriatic skin, which we validated using spatial transcriptomic data. We further showed that expression of the keratinocyte-specific lncRNA, AC020916.1, upregulated in lesional skin, is significantly correlated with expressions of genes participating in cell proliferation/epidermal differentiation, including SPRR2E and transcription factor ZFP36, particularly in the psoriatic skin. Our study highlights the potential for using single-cell RNA sequencing to profile skin-expressing lncRNA transcripts and to infer their cellular origins, providing a crucial approach that can be applied to the study of other inflammatory skin conditions.","PeriodicalId":94239,"journal":{"name":"The Journal of investigative dermatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Benzene Presence and Formation in Benzoyl Peroxide Drug Products. 评估过氧化苯甲酰药物产品中苯的存在和形成。

The Journal of investigative dermatology Pub Date : 2024-09-27 DOI: 10.1016/j.jid.2024.09.009

Kaury Kucera, Nicola Zenzola, Amber Hudspeth, Mara Dubnicka, Wolfgang Hinz, Christopher G Bunick, Michael Girardi, Arash Dabestani, David Y Light

{"title":"Evaluation of Benzene Presence and Formation in Benzoyl Peroxide Drug Products.","authors":"Kaury Kucera, Nicola Zenzola, Amber Hudspeth, Mara Dubnicka, Wolfgang Hinz, Christopher G Bunick, Michael Girardi, Arash Dabestani, David Y Light","doi":"10.1016/j.jid.2024.09.009","DOIUrl":"https://doi.org/10.1016/j.jid.2024.09.009","url":null,"abstract":"The potent carcinogen, benzene, is a known degradation product of benzoyl peroxide (BPO) and was recently reported to form when BPO drug products, used for acne and rosacea treatment, are incubated at body temperature and elevated temperatures expected during storage and transportation. This study provides evidence for a wide range of benzene concentrations (0.16 ppm to 35.30 ppm) detected by GC-MS in 111 over-the-counter BPO drug products tested and maintained at room temperature. A prescription encapsulated BPO drug product was stability tested at cold (2°C) and elevated temperature (50°C), resulting in no apparent benzene formation at 2°C, and high levels of benzene formation at 50°C, suggesting that encapsulation technology may not stabilize BPO drug products but cold storage may greatly reduce benzene formation. Face model experiments where BPO drug product was applied to PolyMethyl MethAcrylate (PMMA) photoprotection test skin plates and benzene was detected in surrounding air by SIFT-MS, showed detectable benzene through evaporation and substantial benzene formation when exposed to UV light at levels below peak sunlight. Results suggest that potential benzene exposure from formation during BPO drug product use poses significant risks independent of the starting benzene concentration.","PeriodicalId":94239,"journal":{"name":"The Journal of investigative dermatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and Cellular Function of p63 in Skin Development and Genetic Diseases. p63 在皮肤发育和遗传疾病中的分子和细胞功能。

The Journal of investigative dermatology Pub Date : 2024-09-26 DOI: 10.1016/j.jid.2024.08.011

Daniela Di Girolamo, Enzo Di Iorio, Caterina Missero

引用次数: 0

Estimation of Body Mass Index from 3-Dimensional Total Body Photography. 通过三维全身摄影估算体重指数（BMI）。

The Journal of investigative dermatology Pub Date : 2024-09-26 DOI: 10.1016/j.jid.2024.06.1294

Sam Kahler, Brigid Betz-Stablein, Fabian Lee, Joachim Torrano, Monika Janda, Clare Primiero, H Peter Soyer, Dilki Jayasinghe

引用次数: 0

Single-Cell RNA Sequencing Reveals Molecular Signatures that Distinguish Allergic from Irritant Contact Dermatitis. 单细胞 RNA 序列分析揭示了区分过敏性接触性皮炎和刺激性接触性皮炎的分子特征。

The Journal of investigative dermatology Pub Date : 2024-09-26 DOI: 10.1016/j.jid.2024.09.008

Michael L Frisoli, Wei-Che C Ko, Nuria Martinez, Khashayar Afshari, Yuqing Wang, Manuel Garber, John E Harris

{"title":"Single-Cell RNA Sequencing Reveals Molecular Signatures that Distinguish Allergic from Irritant Contact Dermatitis.","authors":"Michael L Frisoli, Wei-Che C Ko, Nuria Martinez, Khashayar Afshari, Yuqing Wang, Manuel Garber, John E Harris","doi":"10.1016/j.jid.2024.09.008","DOIUrl":"10.1016/j.jid.2024.09.008","url":null,"abstract":"Allergic contact dermatitis (ACD) is a pruritic skin disease caused by environmental chemicals that induce cell-mediated skin inflammation within susceptible individuals. Irritant contact dermatitis (ICD) is caused by direct damage to the skin barrier by environmental insults. Diagnosis can be challenging because both types of contact dermatitis can appear similar by visual examination, and histopathological analysis does not reliably distinguish ACD from ICD. To discover specific biomarkers of ACD and ICD, we characterized the transcriptomic and proteomic changes that occur within the skin during each type of contact dermatitis. We induced ACD and ICD in healthy human volunteers and sampled skin using a nonscarring suction blister biopsy method that collects interstitial fluid and cellular infiltrate. Single-cell RNA sequencing analysis revealed that cell-specific transcriptome differences rather than cell-type proportions best distinguished ACD from ICD. Allergy-specific genes were associated with upregulation of IFNG, and cell signaling network analysis implicated several other genes such as IL4, despite their low expression levels. We validated transcriptomic differences with proteomic assays on blister fluid and trained a logistic regression model on skin interstitial fluid proteins that could distinguish ACD from ICD and healthy control skin with 93% sensitivity and 93% specificity.","PeriodicalId":94239,"journal":{"name":"The Journal of investigative dermatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Significant Role of Autophagy in Melanosomal Degradation of Dermal Macrophages: Therapeutic Insight Regarding Hyperpigmentation with Uncertain Etiology. 自噬在真皮巨噬细胞黑质体降解中的重要作用--对病因不明的色素沉着症的治疗启示

The Journal of investigative dermatology Pub Date : 2024-09-25 DOI: 10.1016/j.jid.2024.09.007

Kisumi Takiguchi, Kazunori Yokoi, Daiki Murase, Masafumi Yokota, Keigo Kawabata, Yoshito Takahashi, Satoshi Minami, Shuhei Nakamura, Tamotsu Yoshimori, Rei Watanabe, Manabu Fujimoto, Atsushi Tanemura

引用次数: 0

Mouse Models of Itch. 小鼠瘙痒模型

The Journal of investigative dermatology Pub Date : 2024-09-25 DOI: 10.1016/j.jid.2024.08.018

Daniel Yassky, Brian S Kim

引用次数: 0

Type XVII Collagen-Specific CD4⁺ T Cells Induce Bullous Pemphigoid by Producing IL-5. XVII型胶原蛋白特异性CD4+T细胞通过产生IL-5诱导大疱性类天疱疮。

The Journal of investigative dermatology Pub Date : 2024-09-24 DOI: 10.1016/j.jid.2024.08.026

Norihiro Yoshimoto, Ken Muramastsu, Takamasa Ito, Miao Zheng, Kentaro Izumi, Ken Natsuga, Hiroaki Iwata, Yoshinori Hasegawa, Hideyuki Ujiie

{"title":"Type XVII Collagen-Specific CD4+ T Cells Induce Bullous Pemphigoid by Producing IL-5.","authors":"Norihiro Yoshimoto, Ken Muramastsu, Takamasa Ito, Miao Zheng, Kentaro Izumi, Ken Natsuga, Hiroaki Iwata, Yoshinori Hasegawa, Hideyuki Ujiie","doi":"10.1016/j.jid.2024.08.026","DOIUrl":"10.1016/j.jid.2024.08.026","url":null,"abstract":"Bullous pemphigoid is an autoimmune subepidermal blistering disease caused by anti-type XVII collagen (COL17) antibodies. Bullous pemphigoid has some immunological features such as eosinophilic infiltration and the deposition of IgE autoantibodies in the skin; however, the mechanism behind such features remains largely unclear. We focused on the autoantigen-specific CD4+ T cells, which are considered to regulate immune response. We established COL17-specific CD4+ T cell lines in vitro. Wild-type mice were immunized with synthesized peptides that include a pathogenic epitope of COL17, and lymphocytes were subjected to a limiting dilution assay. We established 5 T cell lines and examined the pathogenicity by transferring them with COL17-primed B cells into Rag-2-/-/COL17-humanized mice that express human COL17 but not mouse COL17 in the skin. Notably, 3 lines induced bullous pemphigoid-like skin changes associated with subepidermal separation and eosinophilic infiltration histologically and the production of anti-COL17 antibodies. The other 2 lines did not induce such phenotypes. RNA-sequencing analysis revealed that T helper 2 cytokines, particularly IL-5, were highly expressed in the pathogenic T-cell lines. Anti-IL-5 antibody administration significantly reduced the skin changes and attenuated the production of autoantibodies. Thus, the production of IL-5 is critical for COL17-specific CD4+ T cells to induce bullous pemphigoid phenotypes in vivo.","PeriodicalId":94239,"journal":{"name":"The Journal of investigative dermatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hidradenitis Suppurativa Tunnels Invasive Transcriptional Signature. 化脓性扁桃体炎隧道侵袭转录特征

The Journal of investigative dermatology Pub Date : 2024-09-24 DOI: 10.1016/j.jid.2024.04.037

Stephan M Caucheteux, Vincent Piguet

引用次数: 0