Polish journal of microbiology最新文献

{"title":"A Mendelian Randomization Study: Roles of Gut Microbiota in Sepsis - Who is the Angle?","authors":"Yeping Bian, Jian Xu, Xiaojing Deng, Suming Zhou","doi":"10.33073/pjm-2024-006","DOIUrl":"10.33073/pjm-2024-006","url":null,"abstract":"<p><p>Gut microbiota (GM) is a crucial underlying player during sepsis pathogenesis. However, the causal relationship is unclear and remains to be determined. A two-sample Mendelian randomization study was implemented. The statistical data about sepsis together with GM summarized from genome-wide association studies were evaluated. Instrumental variables were defined as single-nucleotide polymorphisms with prominent correlations with exposure. The inverse-variance-weighted test was employed as a major approach of Mendelian randomization analysis to estimate of causal relationships. The inverse-variance-weighted analysis results demonstrated that at different taxa levels, Actinobacteria and <i>Bifidobacteriaceae</i> influence sepsis. Actinobacteria had negative relationships to sepsis risk at the phylum (β = -0.34, SE = 0.10, <i>p</i> = 0.0008) and class (β = -0.23, SE = 0.07, <i>p</i> = 0.0011) levels in outcome coded ieu-b-69. Actinobacteria at the phylum level (β = -0.22, SE = 0.10, <i>p</i> = 0.027) was also negatively associated with sepsis in outcome coded ieu-b-4980. <i>Bifidobacteriaceae</i> at the order (β = -0.20, SE = 0.06, <i>p</i> = 0.0021), family (β = -0.20, SE = 0.06, <i>p</i> = 0.0021), and genus (β = -0.20, SE = 0.06, <i>p</i> = 0.0007) levels were all negatively correlated with the risk of sepsis in outcome coded ieu-b-69. The results of the Wald ratio model showed that <i>Tyzzerella</i> genus (OR (95%CI) = 0.6902[0.4907,0.9708], <i>p</i> = 0.0331) and Gastranaerophilales order (OR (95%CI) = 0.5907[0.3516,0.9926], <i>p</i> = 0.0468) were negatively connected with sepsis. This study implied at different taxa levels Actinobacteria and <i>Bifidobacteriaceae</i>, <i>Tyzzerella</i> genus, and Gastranaerophilales order have a causal relationship with sepsis, indicating that they are protective factors for the incidence of sepsis.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":"73 1","pages":"49-57"},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10911657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a.","authors":"Xinyi Hu, Pei He, Tong Jiang, Jilu Shen","doi":"10.33073/pjm-2024-009","DOIUrl":"10.33073/pjm-2024-009","url":null,"abstract":"<p><p>Norovirus is highly infectious and rapidly transmissible and represents a major pathogen of sporadic cases and outbreaks of acute gastroenteritis worldwide, causing a substantial disease burden. Recent years have witnessed a dramatic increase in norovirus outbreaks in China, significantly higher than in previous years, among which GII norovirus is the predominant prevalent strain. Therefore, rapid norovirus diagnosis is critical for clinical treatment and transmission control. Hence, we developed a molecular assay based on RPA combined with the CRISPER-CAS12a technique targeting the conserved region of the GII norovirus genome, the results of which could be displayed by fluorescence curves and immunochromatographic lateral-flow test strips. The reaction only required approximately 50 min, and the results were visible by the naked eye with a sensitivity reaching 10² copies/μl. Also, our method does not cross-react with other common pathogens that cause intestinal diarrhea. Furthermore, this assay was easy to perform and inexpensive, which could be widely applied for detecting norovirus in settings including medical institutions at all levels, particularly township health centers in low-resource areas.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":"73 1","pages":"89-97"},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10911698/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased Proteolytic Activity of <i>Serratia marcescens</i> Clinical Isolate HU1848 Is Associated with Higher <i>eepR</i> Expression.","authors":"Karla L De Anda-Mora, Faviola Tavares-Carreón, Carlos Alvarez, Samantha Barahona, Miguel A Becerril-García, Rogelio J Treviño-Rangel, Rodolfo García-Contreras, Angel Andrade","doi":"10.33073/pjm-2024-002","DOIUrl":"10.33073/pjm-2024-002","url":null,"abstract":"<p><p><i>Serratia marcescens</i> is a global opportunistic pathogen. <i>In vitro</i> cytotoxicity of this bacterium is mainly related to metalloprotease serralysin (PrtS) activity. Proteolytic capability varies among the different isolates. Here, we characterized protease production and transcriptional regulators at 37°C of two <i>S. marcescens</i> isolates from bronchial expectorations, HU1848 and SmUNAM836. As a reference strain the insect pathogen <i>S. marcescens</i> Db10 was included. Zymography of supernatant cultures revealed a single (SmUNAM836) or double proteolytic zones (HU1848 and Db10). Mass spectrometry confirmed the identity of PrtS and the serralysin-like protease SlpB from supernatant samples. Elevated proteolytic activity and <i>prtS</i> expression were evidenced in the HU1848 strain through azocasein degradation and qRT-PCR, respectively. Evaluation of transcriptional regulators revealed higher <i>eepR</i> expression in HU1848, whereas <i>cpxR</i> and <i>hexS</i> transcriptional levels were similar between studied strains. Higher <i>eepR</i> expression in HU1848 was further confirmed through an <i>in vivo</i> transcriptional assay. Moreover, two putative CpxR binding motifs were identified within the <i>eepR</i> regulatory region. EMSA validated the interaction of CpxR with both motifs. The evaluation of <i>eepR</i> transcription in a <i>cpxR</i> deletion strain indicated that CpxR negatively regulates <i>eepR</i>. Sequence conservation suggests that regulation of <i>eepR</i> by CpxR is common along <i>S. marcescens</i> species. Overall, our data incorporates CpxR to the complex regulatory mechanisms governing <i>eepR</i> expression and associates the increased proteolytic activity of the HU1848 strain with higher <i>eepR</i> transcription. Based on the global impact of EepR in secondary metabolites production, our work contributes to understanding virulence factors variances across <i>S. marcescens</i> isolates.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":"73 1","pages":"11-20"},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10911700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Resistance and the Prevalence of the Panton-Valentine Leukocidin Gene among Clinical Isolates of <i>Staphylococcus aureus</i> in Lithuania.","authors":"Agnė Kirkliauskienė, Jonas Kriščiūnas, Jolanta Miciulevičienė, Daiva Radzišauskienė, Tomas Kačergius, Maksim Bratchikov, Lina Kaplerienė","doi":"10.33073/pjm-2024-003","DOIUrl":"10.33073/pjm-2024-003","url":null,"abstract":"<p><p>This study aimed to determine resistance to antimicrobials of <i>Staphylococcus aureus</i> strains isolated from clinical specimens in Lithuanian hospitals and to identify the genes conferring resistance and virulence. The study was carried out from June 2019 to September 2021. <i>S. aureus</i> strains were isolated from skin, soft tissues, blood, lower respiratory tract, urine and other specimens. Antibiotic susceptibility testing was performed using the disc diffusion method according to EUCAST guidelines. All isolates were analyzed for detection of the <i>ermA</i>, <i>ermC</i>, <i>mecA</i>, <i>mecC</i>, <i>tetK</i>, <i>tetM</i>, and <i>lukF-PV</i> genes by multiplex real-time PCR. The 16S rRNA coding sequence was applied as an internal PCR control. Altogether, 745 <i>S. aureus</i> strains were analyzed. Antimicrobial susceptibility testing revealed that all isolates were susceptible to rifampin and vancomycin. Of the 745 strains, 94.8% were susceptible to tetracycline, 94.5% to clindamycin, and 88.3% to erythromycin. The lowest susceptibility rate was found for penicillin (25.8%). Six percent of the tested strains were methicillin-resistant <i>S. aureus</i> (MRSA). The majority of methicillin-resistant strains were isolated from skin and soft tissues (73.3%), with a smaller portion isolated from blood (17.8%) and respiratory tract (8.9%). The <i>ermC</i> gene was detected in 41.1% of erythromycin-resistant <i>S. aureus</i> strains, whereas <i>ermA</i> was detected in 32.2% of erythromycin-resistant <i>S. aureus</i> strains. 69.2% of tetracycline-resistant <i>S. aureus</i> strains had <i>tetK</i> gene, and 28.2% had <i>tetM</i> gene. 7.3% of <i>S. aureus</i> isolates harbored <i>lukF-PV</i> gene. The frequency of the <i>pvl</i> gene detection was significantly higher in MRSA isolates than in methicillin-susceptible <i>S. aureus</i> isolates (<i>p</i> < 0.0001).</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":"73 1","pages":"21-28"},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10911699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, Identification, Antimicrobial Resistance, Genotyping, and Whole-Genome Sequencing Analysis of <i>Salmonella</i> Enteritidis Isolated from a Food-Poisoning Incident.","authors":"Zhuru Hou, Benjin Xu, Ling Liu, Rongrong Yan, Jinjing Zhang","doi":"10.33073/pjm-2024-008","DOIUrl":"10.33073/pjm-2024-008","url":null,"abstract":"<p><p><i>Salmonella enterica</i> is a common pathogen in humans and animals that causes food poisoning and infection, threatening public health safety. We aimed to investigate the genome structure, drug resistance, virulence characteristics, and genetic relationship of a <i>Salmonella</i> strain isolated from patients with food poisoning. The pathogen strain 21A was collected from the feces of patients with food poisoning, and its minimum inhibitory concentration against commonly used antibiotics was determined using the strip test and Kirby-Bauer disk methods. Subsequently, WGS analysis was used to reveal the genome structural characteristics and the carrying status of resistance genes and virulence genes of strain 21A. In addition, an MLST-based minimum spanning tree and an SNP-based systematic spanning tree were constructed to investigate its genetic evolutionary characteristics. The strain 21A was identified by mass spectrometry as <i>S. enterica</i>, which was found to show resistance to ampicillin, piperacillin, sulbactam, levofloxacin, and ciprofloxacin. The WGS and bioinformatics analyses revealed this strain as <i>Salmonella</i> Enteritidis belonging to ST11, which is common in China, containing various resistance genes and significant virulence characteristics. Strain 21A was closely related to the SJTUF strains, a series strains from animal, food and clinical sources, as well as from Shanghai, China, which were located in the same evolutionary clade. According to the genetic makeup of strain 21A, the change G > A was found to be the most common variation. We have comprehensively analyzed the genomic characteristics, drug resistance phenotype, virulence phenotype, and genetic evolution relationship of <i>S</i>. Enteritidis strain 21A, which will contribute towards an in-depth understanding of the pathogenic mechanism of <i>S</i>. Enteritidis and the effective prevention and control of foodborne diseases.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":"73 1","pages":"69-89"},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10911658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and metabolic characterization of petroleum hydrocarbons degrading <i>Bacillus cereus</i>.","authors":"Nadia Hussain, Fatima Muccee, Muhammad Hammad, Farhan Mohiuddin, Saboor Muarij Bunny, Aansa Shahab","doi":"10.33073/pjm-2024-012","DOIUrl":"10.33073/pjm-2024-012","url":null,"abstract":"<p><p>Hydrocarbon constituents of petroleum are persistent, bioaccumulated, and bio-magnified in living tissues, transported to longer distances, and exert hazardous effects on human health and the ecosystem. Bioaugmentation with microorganisms like bacteria is an emerging approach that can mitigate the toxins from environmental sources. The present study was initiated to target the petroleum-contaminated soil of gasoline stations situated in Lahore. Petroleum degrading bacteria were isolated by serial dilution method followed by growth analysis, biochemical and molecular characterization, removal efficiency estimation, metabolites extraction, and GC-MS of the metabolites. Molecular analysis identified the bacterium as Bacillus cereus, which exhibited maximum growth at 72 hours and removed 75% petroleum. Biochemical characterization via the Remel RapID^™ ONE panel system showed positive results for arginine dehydrolase (ADH), ornithine decarboxylase (ODC), lysine decarboxylase (LDC), <i>o</i>-nitrophenyl-β-D-galactosidase (ONPG), <i>p</i>-nitrophenyl-β-D-glucosidase (βGLU), <i>p</i>-nitrophenyl-N-acetyl-β-D-glucosaminidase (NAG), malonate (MAL), adonitol fermentation (ADON), and tryptophane utilization (IND). GC-MS-based metabolic profiling identified alcohols (methyl alcohol, <i>o</i>-, <i>p</i>- and <i>m</i>-cresols, catechol, and 3-methyl catechol), aldehydes (methanone, acetaldehyde, and <i>m</i>-tolualdehyde), carboxylic acid (methanoic acid, <i>cis,cis</i>-muconic acid, cyclohexane carboxylic acid and benzoic acid), conjugate bases of carboxylic acids (benzoate, <i>cis,cis</i>-muconate, 4-hydroxybenzoate, and pyruvate) and cycloalkane (cyclohexene). It suggested the presence of methane, methylcyclohexane, toluene, xylene, and benzene degradation pathways in <i>B. cereus</i>.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":"73 1","pages":"107-120"},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10911661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection of a Pathogenic <i>Entamoeba</i> among Symptomatic Children in Eastern Kurdistan of Iraq.","authors":"Sham Jamil Abdullah, Shahnaz Abdulkader Ali","doi":"10.33073/pjm-2024-010","DOIUrl":"10.33073/pjm-2024-010","url":null,"abstract":"<p><p><i>Entamoeba histolytica</i> infects the large intestine of humans, causing a spectrum of clinical appearances ranging from asymptomatic colonization to severe intestinal and extra-intestinal disease. The parasite is identical microscopically to commensal nonpathogenic amoeba. To detect the pathogenic <i>Entamoeba</i> and estimate the precise prevalence of the parasite among the symptomatic pediatric population using molecular techniques. 323 fecal samples were collected from symptomatic children admitted to Sulaimani Pediatric Teaching Hospital, Sulaimaniyah Province, Iraq, from June to October 2021. A structured, validated questionnaire was prepared and used to report participants' gender, residency, and drinking water source. Then, stool samples were microscopically examined, and the positive samples were submitted to molecular analysis by amplifying the 18s rRNA gene using nested PCR to differentiate <i>E. histolytica</i> from other nonpathogenic <i>Entamoeba</i>. Finally, gene sequences were done to confirm the species. Microscopically, 58 positive samples represented <i>Entamoeba</i> species infection rate of 18% among symptomatic patients. However, only 18 samples were positive for <i>E. histolytica</i> based on molecular methods, which accounts for 31% of the positive by microscopy and 5.6% among the 323 symptomatic populations. NCBI, available in their database, gives the gene sequence and accession number. Patients' sociodemographic data and water sources were directly related to the infection rate. Classical microscopic examination provides a misleading profile about the prevalence of <i>E. histolytica</i> in an endemic region that might lead to unnecessary treatments and a lack of appropriate management for patients.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":"73 1","pages":"99-105"},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10911696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol and Growth Promotion Potential of <i>Bacillus subtilis</i> CTXW 7-6-2 against <i>Rhizoctonia solani</i> that Causes Tobacco Target Spot Disease.","authors":"Ning Huang, Xin Jin, Jin-Tao Wen, Yi-Fei Zhang, Xu Yang, Guang-Yu Wei, Yi-Kun Wang, Min Qin","doi":"10.33073/pjm-2024-004","DOIUrl":"10.33073/pjm-2024-004","url":null,"abstract":"<p><p>Fungal diseases form perforated disease spots in tobacco plants, resulting in a decline in tobacco yield and quality. The present study investigated the antagonistic effect of <i>Bacillus subtilis</i> CTXW 7-6-2 against <i>Rhizoctonia solani</i>, its ability to promote the growth of tobacco seedlings, and the expression of disease resistance-related genes for efficient and eco-friendly plant disease control. Our results showed that CTXW 7-6-2 had the most vigorous growth after being cultured for 96 h, and its rate of inhibition of <i>R. solani</i> growth <i>in vitro</i> was 94.02%. The volatile compounds produced by CTXW 7-6-2 inhibited the growth of <i>R. solani</i> significantly (by 96.62%). The fungal growthinhibition rate of the <i>B. subtilis</i> CTXW 7-6-2 broth obtained after high-temperature and no-high-temperature sterile fermentation was low, at 50.88% and 54.63%, respectively. The lipopeptides extracted from the <i>B. subtilis</i> CTXW 7-6-2 fermentation broth showed a 74.88% fungal growth inhibition rate at a concentration of 100 mg/l. Scanning and transmission electron microscopy showed some organelle structural abnormalities, collapse, shrinkage, blurring, and dissolution in the <i>R. solani</i> mycelia. In addition, CTXW 7-6-2 increased tobacco seedling growth and improved leaf and root weight compared to the control. After CTXW 7-6-2 inoculation, tobacco leaves showed the upregulation of the <i>PDF1.2</i>, <i>PPO</i>, and <i>PAL</i> genes, which are closely related to target spot disease resistance. In conclusion, <i>B. subtilis</i> CTXW 7-6-2 may be an efficient biological control agent in tobacco agriculture and enhance plant growth potential.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":"73 1","pages":"29-38"},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10911660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the BioFire® FilmArray® Pneumonia <i>plus</i> Panel for Detecting Bacterial Etiological Agents of Lower Respiratory Tract Infections in an Oncologic Hospital. Comparison with Conventional Culture Method.","authors":"Maria Teresa Szymankiewicz, Anna Szczepanska, Elzbieta Stefaniuk","doi":"10.33073/pjm-2023-035","DOIUrl":"10.33073/pjm-2023-035","url":null,"abstract":"<p><p>Conventional methods used to determine pneumonia pathogens are characterized by low sensitivity and long turnaround times. Introducing new tests with better parameters in patients at higher risk of infections is highly anticipated. The results of the conventional quantitative culture method (CM) in determining the bacterial etiology of pneumonia were compared with the results of the Pneumonia <i>plus</i> Panel test (PNP; BioFire® Diagnostics, USA) in 79 samples of bronchoalveolar lavage (BAL). Materials were collected from 79 patients with suspected pneumonia treated in an oncologic hospital due to solid tumors. Only 16/79 BAL samples (20.3%) were true positive (TP) for bacterial etiology in CM vs. 27/79 samples (34.2%) true positive in the PNP test. The total agreement between methods of interpreting the result (positive or negative) was 84.8%. The most prevalent pathogens in both methods were <i>Staphylococcus aureus</i>, followed by <i>Escherichia coli, Pseudomonas aeruginosa</i>, and <i>Haemophilus influenzae</i>. The PNP test identified several respiratory pathogens that were not grown in culture. The semiquantitative value reported by the PNP test was higher than that reported by culture. The PNP test vs. combined test (PNP test and CM methods) demonstrated positive predictive value (PPV) and negative predictive value (NPV) values of 100.0% and 98.1%, and the sensitivity and specificity were 96.4% and 100.0%. The PNP test is a good tool for determining the etiology of bacterial pneumonia and may support the care of an oncologic patient. However, further large-sample studies are needed to research in strictly defined groups of oncologic patients.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":" ","pages":"391-398"},"PeriodicalIF":0.0,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10725156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41184590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Awareness of the Interplay Between the Gut Microbiota and Circadian Rhythms.","authors":"Xiaoxiao Pang, Long Chen, Guoxin Xu","doi":"10.33073/pjm-2023-046","DOIUrl":"10.33073/pjm-2023-046","url":null,"abstract":"<p><p>Circadian rhythms influence various aspects of the biology and physiology of the host, such as food intake and sleep/wake cycles. In recent years, an increasing amount of genetic and epidemiological data has shown that the light/dark cycle is the main cue that regulates circadian rhythms. Other factors, including sleep/wake cycles and food intake, have necessary effects on the composition and rhythms of the gut microbiota. Interestingly, the gut microbiota can affect the circadian rhythm of hosts in turn through contact-dependent and contact-independent mechanisms. Furthermore, the gut microbiota has been shown to regulate the sleep/wake cycles through gut-brain-microbiota interaction. In addition to diabetes, the gut microbiota can also intervene in the progression of neuro- degenerative diseases through the gut-brain-microbiota interaction, and also in other diseases such as hypertension and rheumatoid arthritis, where it is thought to have a spare therapeutic potential. Even though fecal microbiota transplantation has good potential for treating many diseases, the risk of spreading intestinal pathogens should not be ignored.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":"72 4","pages":"355-363"},"PeriodicalIF":0.0,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10725168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138816109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}