{"title":"中国山东某三级甲等医院耐 mcr-1 阳性多粘菌素 B 大肠埃希菌产生广谱 β-内酰胺酶 (ESBL) 的分子流行病学。","authors":"Yue Liu, Qian Wang, Ting Qi, Meng Zhang, Ran Chen, Zaifeng Si, Jinmei Li, Yan Jin, Qingbing Xu, Ping Li, Yingying Hao","doi":"10.33073/pjm-2024-032","DOIUrl":null,"url":null,"abstract":"<p><p><i>Escherichia coli</i>, a rod-shaped Gram-negative bacterium, is a significant causative agent of severe clinical bacterial infections. This study aimed to analyze the epidemiology of extended-spectrum β-lactamase (ESBL)-producing <i>mcr-1</i> -positive <i>E. coli</i> in Shandong, China. We collected 668 non-duplicate ESBL-producing <i>E. coli</i> strains from clinical samples at Shandong Provincial Hospital between January and December 2018, and estimated their minimum inhibitory concentrations (MICs) using a VITEK<sup>®</sup> 2 compact system and broth microdilution. Next-generation sequencing and bioinformatic analyses identified the <i>mcr-1</i> gene and other resistance genes in the polymyxin B-resistant strains. The conjugation experiment assessed the horizontal transfer capacity of the <i>mcr-</i>1 gene. Of the strains collected, 24 polymyxin B-resistant strains were isolated with a positivity rate of 3.59% and among the 668 strains, 19 clinical strains carried the mobile colistin resistance gene <i>mcr-1</i>, with a positivity rate of approximately 2.8%. All 19 clinical strains were resistant to ampicillin, cefazolin, ceftriaxone, ciprofloxacin, levofloxacin, and polymyxin B. Seventeen strains successfully transferred the <i>mcr-1</i> gene into <i>E. coli</i> J53. All transconjugants were resistant to polymyxin B, and carried the drug resistance gene <i>mcr-1</i>. The 19 clinical strains had 14 sequence types (STs), with ST155 (n = 4) being the most common. The whole-genome sequencing results of pECO-POL-29_mcr1 revealed that no IS<i>Apl1</i> insertion sequences were found on either side of the <i>mcr-1</i> gene. Our study uncovered the molecular epidemiology of <i>mcr-1</i>-carrying ESBL-producing <i>E. coli</i> in the region and suggested horizontal transmission mediated by plasmids as the main mode of <i>mcr-1</i> transmission.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11395425/pdf/","citationCount":"0","resultStr":"{\"title\":\"Molecular Epidemiology of <i>mcr-1</i>-Positive Polymyxin B-Resistant <i>Escherichia coli</i> Producing Extended-Spectrum β-Lactamase (ESBL) in a Tertiary Hospital in Shandong, China.\",\"authors\":\"Yue Liu, Qian Wang, Ting Qi, Meng Zhang, Ran Chen, Zaifeng Si, Jinmei Li, Yan Jin, Qingbing Xu, Ping Li, Yingying Hao\",\"doi\":\"10.33073/pjm-2024-032\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Escherichia coli</i>, a rod-shaped Gram-negative bacterium, is a significant causative agent of severe clinical bacterial infections. This study aimed to analyze the epidemiology of extended-spectrum β-lactamase (ESBL)-producing <i>mcr-1</i> -positive <i>E. coli</i> in Shandong, China. We collected 668 non-duplicate ESBL-producing <i>E. coli</i> strains from clinical samples at Shandong Provincial Hospital between January and December 2018, and estimated their minimum inhibitory concentrations (MICs) using a VITEK<sup>®</sup> 2 compact system and broth microdilution. Next-generation sequencing and bioinformatic analyses identified the <i>mcr-1</i> gene and other resistance genes in the polymyxin B-resistant strains. The conjugation experiment assessed the horizontal transfer capacity of the <i>mcr-</i>1 gene. Of the strains collected, 24 polymyxin B-resistant strains were isolated with a positivity rate of 3.59% and among the 668 strains, 19 clinical strains carried the mobile colistin resistance gene <i>mcr-1</i>, with a positivity rate of approximately 2.8%. All 19 clinical strains were resistant to ampicillin, cefazolin, ceftriaxone, ciprofloxacin, levofloxacin, and polymyxin B. Seventeen strains successfully transferred the <i>mcr-1</i> gene into <i>E. coli</i> J53. All transconjugants were resistant to polymyxin B, and carried the drug resistance gene <i>mcr-1</i>. The 19 clinical strains had 14 sequence types (STs), with ST155 (n = 4) being the most common. The whole-genome sequencing results of pECO-POL-29_mcr1 revealed that no IS<i>Apl1</i> insertion sequences were found on either side of the <i>mcr-1</i> gene. Our study uncovered the molecular epidemiology of <i>mcr-1</i>-carrying ESBL-producing <i>E. coli</i> in the region and suggested horizontal transmission mediated by plasmids as the main mode of <i>mcr-1</i> transmission.</p>\",\"PeriodicalId\":94173,\"journal\":{\"name\":\"Polish journal of microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11395425/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polish journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33073/pjm-2024-032\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/9/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polish journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33073/pjm-2024-032","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
Molecular Epidemiology of mcr-1-Positive Polymyxin B-Resistant Escherichia coli Producing Extended-Spectrum β-Lactamase (ESBL) in a Tertiary Hospital in Shandong, China.
Escherichia coli, a rod-shaped Gram-negative bacterium, is a significant causative agent of severe clinical bacterial infections. This study aimed to analyze the epidemiology of extended-spectrum β-lactamase (ESBL)-producing mcr-1 -positive E. coli in Shandong, China. We collected 668 non-duplicate ESBL-producing E. coli strains from clinical samples at Shandong Provincial Hospital between January and December 2018, and estimated their minimum inhibitory concentrations (MICs) using a VITEK® 2 compact system and broth microdilution. Next-generation sequencing and bioinformatic analyses identified the mcr-1 gene and other resistance genes in the polymyxin B-resistant strains. The conjugation experiment assessed the horizontal transfer capacity of the mcr-1 gene. Of the strains collected, 24 polymyxin B-resistant strains were isolated with a positivity rate of 3.59% and among the 668 strains, 19 clinical strains carried the mobile colistin resistance gene mcr-1, with a positivity rate of approximately 2.8%. All 19 clinical strains were resistant to ampicillin, cefazolin, ceftriaxone, ciprofloxacin, levofloxacin, and polymyxin B. Seventeen strains successfully transferred the mcr-1 gene into E. coli J53. All transconjugants were resistant to polymyxin B, and carried the drug resistance gene mcr-1. The 19 clinical strains had 14 sequence types (STs), with ST155 (n = 4) being the most common. The whole-genome sequencing results of pECO-POL-29_mcr1 revealed that no ISApl1 insertion sequences were found on either side of the mcr-1 gene. Our study uncovered the molecular epidemiology of mcr-1-carrying ESBL-producing E. coli in the region and suggested horizontal transmission mediated by plasmids as the main mode of mcr-1 transmission.