中国山东某三级甲等医院耐 mcr-1 阳性多粘菌素 B 大肠埃希菌产生广谱 β-内酰胺酶 (ESBL) 的分子流行病学。

Polish journal of microbiology Pub Date : 2024-09-13 eCollection Date: 2024-09-01 DOI:10.33073/pjm-2024-032
Yue Liu, Qian Wang, Ting Qi, Meng Zhang, Ran Chen, Zaifeng Si, Jinmei Li, Yan Jin, Qingbing Xu, Ping Li, Yingying Hao
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引用次数: 0

摘要

大肠埃希菌是一种杆状革兰氏阴性菌,是临床严重细菌感染的重要致病菌。本研究旨在分析中国山东产广谱β-内酰胺酶(ESBL)mcr-1阳性大肠埃希菌的流行病学。2018年1月至12月期间,我们从山东省立医院的临床样本中收集了668株不重复的产ESBL大肠杆菌,并使用VITEK® 2紧凑型系统和肉汤微量稀释法估算了它们的最低抑菌浓度(MIC)。下一代测序和生物信息学分析确定了多粘菌素B耐药菌株中的mcr-1基因和其他耐药基因。共轭实验评估了 mcr-1 基因的水平转移能力。在收集到的菌株中,分离出 24 株多粘菌素 B 耐药菌株,阳性率为 3.59%;在 668 株菌株中,19 株临床菌株携带可移动的可乐定耐药基因 mcr-1,阳性率约为 2.8%。所有 19 株临床菌株都对氨苄西林、头孢唑啉、头孢曲松、环丙沙星、左氧氟沙星和多粘菌素 B 具有耐药性。所有转基因菌株都对多粘菌素 B 具有抗药性,并携带抗药性基因 mcr-1。19 株临床菌株有 14 种序列类型(ST),其中 ST155(n = 4)最为常见。pECO-POL-29_mcr1 的全基因组测序结果显示,mcr-1 基因两侧均未发现 ISApl1 插入序列。我们的研究揭示了该地区携带 mcr-1 产 ESBL 大肠杆菌的分子流行病学,并认为质粒介导的水平传播是 mcr-1 的主要传播方式。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular Epidemiology of mcr-1-Positive Polymyxin B-Resistant Escherichia coli Producing Extended-Spectrum β-Lactamase (ESBL) in a Tertiary Hospital in Shandong, China.

Escherichia coli, a rod-shaped Gram-negative bacterium, is a significant causative agent of severe clinical bacterial infections. This study aimed to analyze the epidemiology of extended-spectrum β-lactamase (ESBL)-producing mcr-1 -positive E. coli in Shandong, China. We collected 668 non-duplicate ESBL-producing E. coli strains from clinical samples at Shandong Provincial Hospital between January and December 2018, and estimated their minimum inhibitory concentrations (MICs) using a VITEK® 2 compact system and broth microdilution. Next-generation sequencing and bioinformatic analyses identified the mcr-1 gene and other resistance genes in the polymyxin B-resistant strains. The conjugation experiment assessed the horizontal transfer capacity of the mcr-1 gene. Of the strains collected, 24 polymyxin B-resistant strains were isolated with a positivity rate of 3.59% and among the 668 strains, 19 clinical strains carried the mobile colistin resistance gene mcr-1, with a positivity rate of approximately 2.8%. All 19 clinical strains were resistant to ampicillin, cefazolin, ceftriaxone, ciprofloxacin, levofloxacin, and polymyxin B. Seventeen strains successfully transferred the mcr-1 gene into E. coli J53. All transconjugants were resistant to polymyxin B, and carried the drug resistance gene mcr-1. The 19 clinical strains had 14 sequence types (STs), with ST155 (n = 4) being the most common. The whole-genome sequencing results of pECO-POL-29_mcr1 revealed that no ISApl1 insertion sequences were found on either side of the mcr-1 gene. Our study uncovered the molecular epidemiology of mcr-1-carrying ESBL-producing E. coli in the region and suggested horizontal transmission mediated by plasmids as the main mode of mcr-1 transmission.

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