mLife最新文献 - Book学术

Avian tuberculosis identified as the potential disease in an outbreak in wild migratory birds in China.

IF 4.5

mLife Pub Date : 2025-02-27 eCollection Date: 2025-02-01 DOI: 10.1002/mlf2.12164

Chunge Zhang, Liang Wang, Cheng Zhang, Ning Zhang, Heting Sun, Dong Chu, Siyuan Qin, Zhenghai Ma, Marina Gulyaeva, Alexander Shestopalov, Wenjun Liu, George F Gao, Yuhai Bi

引用次数: 0

Genome-wide siRNA library screening identifies human host factors that influence the replication of the highly pathogenic H5N1 influenza virus.

IF 4.5

mLife Pub Date : 2025-02-24 eCollection Date: 2025-02-01 DOI: 10.1002/mlf2.12168

Guangwen Wang, Li Jiang, Jinliang Wang, Qibing Li, Jie Zhang, Fandi Kong, Ya Yan, Yuqin Wang, Guohua Deng, Jianzhong Shi, Guobin Tian, Xianying Zeng, Liling Liu, Zhigao Bu, Hualan Chen, Chengjun Li

{"title":"Genome-wide siRNA library screening identifies human host factors that influence the replication of the highly pathogenic H5N1 influenza virus.","authors":"Guangwen Wang, Li Jiang, Jinliang Wang, Qibing Li, Jie Zhang, Fandi Kong, Ya Yan, Yuqin Wang, Guohua Deng, Jianzhong Shi, Guobin Tian, Xianying Zeng, Liling Liu, Zhigao Bu, Hualan Chen, Chengjun Li","doi":"10.1002/mlf2.12168","DOIUrl":"https://doi.org/10.1002/mlf2.12168","url":null,"abstract":"The global dissemination of H5 avian influenza viruses represents a significant threat to both human and animal health. In this study, we conducted a genome-wide siRNA library screening against the highly pathogenic H5N1 influenza virus, leading us to the identification of 457 cellular cofactors (441 proviral factors and 16 antiviral factors) involved in the virus replication cycle. Gene Ontology term enrichment analysis revealed that the candidate gene data sets were enriched in gene categories associated with mRNA splicing via spliceosome in the biological process, integral component of membrane in the cellular component, and protein binding in the molecular function. Reactome pathway analysis showed that the immune system (up to 63 genes) was the highest enriched pathway. Subsequent comparisons with four previous siRNA library screenings revealed that the overlapping rates of the involved pathways were 8.53%-62.61%, which were significantly higher than those of the common genes (1.85%-6.24%). Together, our genome-wide siRNA library screening unveiled a panorama of host cellular networks engaged in the regulation of highly pathogenic H5N1 influenza virus replication, which may provide potential targets and strategies for developing novel antiviral countermeasures.","PeriodicalId":94145,"journal":{"name":"mLife","volume":"4 1","pages":"55-69"},"PeriodicalIF":4.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of novel components of the Ced and Ups systems in Saccharolobus islandicus REY15A.

IF 4.5

mLife Pub Date : 2025-02-23 eCollection Date: 2025-02-01 DOI: 10.1002/mlf2.12163

Pengju Wu, Mengqi Zhang, Yanlu Kou, Shikuan Liang, Jinfeng Ni, Qihong Huang, Yulong Shen

{"title":"Identification of novel components of the Ced and Ups systems in Saccharolobus islandicus REY15A.","authors":"Pengju Wu, Mengqi Zhang, Yanlu Kou, Shikuan Liang, Jinfeng Ni, Qihong Huang, Yulong Shen","doi":"10.1002/mlf2.12163","DOIUrl":"https://doi.org/10.1002/mlf2.12163","url":null,"abstract":"In Sulfolobales cells, transcription of the Ups (UV-inducible pili of Sulfolobus) and Ced (Crenarchaeal system for exchange of DNA) genes is highly induced by DNA damage, and the two systems play key roles in pili-mediated cell aggregation and chromosomal DNA import, respectively. Ups is composed of UpsA, UpsB, UpsE, and UpsF, while Ced is composed of CedA, CedA1, CedA2, and CedB. So far, how DNA is transported by these systems is far from clear. Here, we report three novel components of the Ced and Ups systems in Saccharolobus islandicus REY15A, CedD (SiRe_1715) and CedE (SiRe_2100), paralogs of CedB and CedA, and UpsC (SiRe_1957), a paralog of UpsA/UpsB. We developed a DNA import and export assay method, by which we revealed that CedD, CedE, and UpsC are essential for DNA import, while CedE and UpsC are also involved in DNA export together with CedA1 and Ups. Microscopic analysis revealed that upsC is involved in cell aggregation like other Ups genes. In addition, we found that cedB and cedD co-occur in the Crenarchaeal genomes that lack virB4, an essential component of type IV secretion system. Interestingly, CedB and CedD share homology to different parts of VirB4 N-terminal domain and form stable homo-oligomers in vitro. Collectively, our results indicate that CedD, CedE, and UpsC are integral components of the Ced and Ups systems in Sulfolobales.","PeriodicalId":94145,"journal":{"name":"mLife","volume":"4 1","pages":"17-28"},"PeriodicalIF":4.5,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthetic evolution of Saccharomyces cerevisiae for biomanufacturing: Approaches and applications.

IF 4.5

mLife Pub Date : 2025-02-23 eCollection Date: 2025-02-01 DOI: 10.1002/mlf2.12167

Zhen Wang, Xianni Qi, Xinru Ren, Yuping Lin, Fanli Zeng, Qinhong Wang

引用次数: 0

The role of PilU in the surface behaviors of Pseudomonas aeruginosa.

IF 4.5

mLife Pub Date : 2025-02-23 eCollection Date: 2025-02-01 DOI: 10.1002/mlf2.12165

Jingchao Zhang, Yan Luo, Yiwu Zong, Shangping Lu, Yi Shi, Fan Jin, Kun Zhao

引用次数: 0

Developing a microfluidic-based epicPCR reveals diverse potential hosts of the mcrA gene in marine cold seep.

IF 4.5

mLife Pub Date : 2025-02-20 eCollection Date: 2025-02-01 DOI: 10.1002/mlf2.12159

Wenli Shen, Danrui Wang, Jiangtao Li, Yue Liu, Yinzhao Wang, Xingsheng Yang, Xi Peng, Bingliang Xie, Lei Su, Ziyan Wei, Qing He, Zhiyi Wang, Kai Feng, Wenbin Du, Ye Deng

{"title":"Developing a microfluidic-based epicPCR reveals diverse potential hosts of the mcrA gene in marine cold seep.","authors":"Wenli Shen, Danrui Wang, Jiangtao Li, Yue Liu, Yinzhao Wang, Xingsheng Yang, Xi Peng, Bingliang Xie, Lei Su, Ziyan Wei, Qing He, Zhiyi Wang, Kai Feng, Wenbin Du, Ye Deng","doi":"10.1002/mlf2.12159","DOIUrl":"https://doi.org/10.1002/mlf2.12159","url":null,"abstract":"Anaerobic methanotrophic (ANME) microbes play a crucial role in the bioprocess of anaerobic oxidation of methane (AOM). However, due to their unculturable status, their diversity is poorly understood. In this study, we established a microfluidics-based epicPCR (Emulsion, Paired Isolation, and Concatenation PCR) to fuse the 16S rRNA gene and mcrA gene to reveal the diversity of ANME microbes (mcrA gene hosts) in three sampling push-cores from the marine cold seep. A total of 3725 16S amplicon sequence variants (ASVs) of the mcrA gene hosts were detected, and classified into 78 genera across 23 phyla. Across all samples, the dominant phyla with high relative abundance (>10%) were the well-known Euryarchaeota, and some bacterial phyla such as Campylobacterota, Proteobacteria, and Chloroflexi; however, the specificity of these associations was not verified. In addition, the compositions of the mcrA gene hosts were significantly different in different layers, where the archaeal hosts increased with the depths of sediments, indicating the carriers of AOM were divergent in depth. Furthermore, the consensus phylogenetic trees of the mcrA gene and the 16S rRNA gene showed congruence in archaea not in bacteria, suggesting the horizontal transfer of the mcrA gene may occur among host members. Finally, some bacterial metagenomes were found to contain the mcrA gene as well as other genes that encode enzymes in the AOM pathway, which prospectively propose the existence of ANME bacteria. This study describes improvements for a potential method for studying the diversity of uncultured functional microbes and broadens our understanding of the diversity of ANMEs.","PeriodicalId":94145,"journal":{"name":"mLife","volume":"4 1","pages":"70-82"},"PeriodicalIF":4.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The endocytic pathway for absorption of exogenous RNAs in Verticillium dahliae.

IF 4.5

mLife Pub Date : 2025-02-07 eCollection Date: 2025-02-01 DOI: 10.1002/mlf2.12149

Chuanhui Liu, Chen Cui, Guanyin Zhou, Feng Gao, Jianhua Zhao, Huishan Guo, Yun Jin

{"title":"The endocytic pathway for absorption of exogenous RNAs in Verticillium dahliae.","authors":"Chuanhui Liu, Chen Cui, Guanyin Zhou, Feng Gao, Jianhua Zhao, Huishan Guo, Yun Jin","doi":"10.1002/mlf2.12149","DOIUrl":"https://doi.org/10.1002/mlf2.12149","url":null,"abstract":"RNAi technologies have been exploited to control viruses, pests, oomycetes, and fungal phytopathogens that cause disasters in host plants, including many agronomically significant crops. Double-stranded RNA (dsRNA) or small interfering RNA (siRNA) has been applied as a trigger for trans-kingdom RNAi between hosts and fungi. However, it is unclear what process mediates RNA uptake by fungi. In this study, by using live-cell imaging, we determined that exogenously synthesized RNA or small RNA (sRNA) was indiscriminately absorbed into Verticillium dahliae, a notorious pathogenic fungus. Moreover, the application of endocytic inhibitors or deletion of endocytic-related genes reduced RNA uptake efficiency, showing that RNA absorption by fungal cells occurs mainly through endocytosis. In addition, we found that the endocytosed fluorescence-labeled RNAs were partly colocalized with endosome marker genes. Overall, our research concluded that exogenous RNA could be assimilated by V. dahliae through the endocytic pathway. Unraveling this cytological mechanism underlying trans-kingdom RNAi holds significant importance, especially considering the fact that RNAi-based strategies targeting pathogenic fungi are increasingly prevalent in the realm of crop protection.","PeriodicalId":94145,"journal":{"name":"mLife","volume":"4 1","pages":"45-54"},"PeriodicalIF":4.5,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reviewer Acknowledgments.

IF 4.5

mLife Pub Date : 2025-01-28 eCollection Date: 2025-02-01 DOI: 10.1002/mlf2.12162

引用次数: 0

A new type of ArsR transcriptional repressor controls transcription of the arsenic resistance operon of Arsenicibacter rosenii SM-1.

IF 4.5

mLife Pub Date : 2025-01-19 eCollection Date: 2025-02-01 DOI: 10.1002/mlf2.12155

Yujie Zhang, Wenjun Wu, Ke Huang, Fang-Jie Zhao

引用次数: 0

Complexation of CcmB with CcmACD safeguards heme translocation for cytochrome c maturation.

IF 4.5

mLife Pub Date : 2025-01-06 eCollection Date: 2025-02-01 DOI: 10.1002/mlf2.12150

Yuanyou Xu, Wei Wang, Qianrou Zhang, Sirui Han, Jiahao Wang, Shihua Wu, Haichun Gao

引用次数: 0