Laboratory medicine最新文献_第8页

The roles played by hsa-miR-223-5p and mutations in the S gene of SARS-CoV-2 in COVID-19. hsa-miR-223-5p 和 SARS-CoV-2 S 基因突变在 COVID-19 中的作用。

Laboratory medicine Pub Date : 2024-08-05 DOI: 10.1093/labmed/lmae066

Zeyad Sadeg Sabbar, Ashraf Kariminik, Maryam Ghane

{"title":"The roles played by hsa-miR-223-5p and mutations in the S gene of SARS-CoV-2 in COVID-19.","authors":"Zeyad Sadeg Sabbar, Ashraf Kariminik, Maryam Ghane","doi":"10.1093/labmed/lmae066","DOIUrl":"https://doi.org/10.1093/labmed/lmae066","url":null,"abstract":"Background: Increased proinflammatory molecules are a main reason for severe symptoms in patients infected with SARS-CoV-2. This study evaluated mutations in the S gene of SARS-CoV-2 and the expression of hsa-miR-223-5p, interleukin 2 receptor α (IL-2Rα), and CCL16 chemokine in hospitalized SARS-CoV-2 infected patients.Design: This is a cross-sectional study.Methods: This study included 75 SARS-CoV-2-infected patients with severe symptoms and 75 age-sex-matched healthy controls. Real-time polymerase chain reaction techniques were used to evaluate the expression levels of hsa-miR-223-5p, IL-2Rα, and CCL16 chemokine. The Sanger technique was used to sequence the S gene of SARS-CoV-2 from positions 23,274 to 23,641.Results: The relative expression of hsa-miR-223-5p was significantly increased whereas that of IL-2Rα was significantly decreased in the SARS-CoV-2 infected patients. Two mutations were found in the S gene of SARS-CoV-2 at positions 23,403 (p.Asp23403Gly) and 23,525 (p.His23525Tyr) of the S gene of SARS-CoV-2.Conclusion: Increased hsa-miR-223-5p may be a main cause for the downregulation of IL-2Rα, which is a main developer of T-regulatory lymphocytes. The mutations in the S gene of SARS-CoV-2-infected patients may affect immune responses to the molecule and alter the avidity of virus-human cell interactions.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141895117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prothrombin deficiency with recurrent subretinal hemorrhage. 凝血酶原缺乏症伴复发性视网膜下出血。

Laboratory medicine Pub Date : 2024-07-30 DOI: 10.1093/labmed/lmae057

Eros Qama, Kritika Krishnamurthy, Joseph J Mulvey, Kenneth G Liu, David L Fernandes, Yanan Fang

{"title":"Prothrombin deficiency with recurrent subretinal hemorrhage.","authors":"Eros Qama, Kritika Krishnamurthy, Joseph J Mulvey, Kenneth G Liu, David L Fernandes, Yanan Fang","doi":"10.1093/labmed/lmae057","DOIUrl":"https://doi.org/10.1093/labmed/lmae057","url":null,"abstract":"Ocular hemorrhage has been recorded in congenital factor deficiencies like hemophilia A, but it has never been documented in prothrombin deficiency. Here, we describe an unusual case of sudden vision loss in the right eye caused by subretinal hemorrhage following a coughing episode in a 67-year-old woman. Notably, the patient underwent left eye enucleation 12 years previously under similar circumstances due to subretinal hemorrhage. During the interview, it was discovered that the patient had a history of prothrombin deficiency, which was subsequently confirmed through laboratory testing. Aside from recurrent ocular bleeding and 1 instance of bleeding following dental extraction in childhood, there is no other history of bleeding. Subsequent molecular studies revealed a homozygous missense mutation at G1499A (Arg500Gln), a variant previously identified as R457Q. Although the likelihood of prothrombin deficiency initiating subretinal hemorrhage is low, it is likely to worsen retinal hemorrhage and contribute to difficulty in controlling bleeding. A comprehensive coagulation workup is essential in patients with ocular hemorrhage. Determining factor II activity should be included in individuals exhibiting variably prolonged prothrombin time and activated partial thromboplastin time with correction in mixing studies. Additional investigations, such as genetic sequencing and family studies, are advised for those with isolated low prothrombin levels.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141857498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between triglyceride to HDL cholesterol ratio and a risk of diabetes mellitus: a systematic review and meta-analysis. 甘油三酯与高密度脂蛋白胆固醇比率与糖尿病风险之间的关系：系统回顾和荟萃分析。

Laboratory medicine Pub Date : 2024-07-27 DOI: 10.1093/labmed/lmae052

Hongjing Zhong, Laiming Luo, Xiaomei Wang, Yao Xiao

{"title":"Association between triglyceride to HDL cholesterol ratio and a risk of diabetes mellitus: a systematic review and meta-analysis.","authors":"Hongjing Zhong, Laiming Luo, Xiaomei Wang, Yao Xiao","doi":"10.1093/labmed/lmae052","DOIUrl":"https://doi.org/10.1093/labmed/lmae052","url":null,"abstract":"Objective: The aim of this study was to investigate the link between the triglyceride-to-high-density lipoprotein cholesterol ratio (TG/HDL-C) and the occurrence of type 2 diabetes mellitus (T2DM).Methods: PubMed, Embase, and Scopus databases were searched for cohort and case-control studies that reported on the link between TG/HDL-C and a risk of T2DM, with no restrictions on criteria used for the definition and categorization of low and high TG/HDL-C ratios.Results: A total of 20 studies were included. There was considerable variability in terms of categorization of low or normal and higher TG/HDL-C ratio among the studies. Patients with high TG/HDL-C ratio had markedly higher risk of developing T2DM compared with patients with low or normal TG/HDL-C. Each unit increase in the ratio correlated with the increased risk of diabetes. Subgroup analysis based on sex showed an increased risk of T2DM in males and females with a high ratio compared with the group with a low/normal ratio.Conclusion: Higher TG/HDL-C ratio correlates with increased risk of T2DM. Despite limitations, the study demonstrates a possible value of using TG/HDL-C ratio as a biomarker for diabetes risk.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Red blood cell alloimmunization in transfused patients with myelodysplastic syndromes: a retrospective study from northern China. 骨髓增生异常综合征输血患者的红细胞同种免疫：一项来自中国北方的回顾性研究。

Laboratory medicine Pub Date : 2024-07-26 DOI: 10.1093/labmed/lmae056

Li Wang, Xiaoxin Xu, Shichun Wang, Ruidong Li, Pengyu Zhang

{"title":"Red blood cell alloimmunization in transfused patients with myelodysplastic syndromes: a retrospective study from northern China.","authors":"Li Wang, Xiaoxin Xu, Shichun Wang, Ruidong Li, Pengyu Zhang","doi":"10.1093/labmed/lmae056","DOIUrl":"https://doi.org/10.1093/labmed/lmae056","url":null,"abstract":"Objective: Alloimmunization against red blood cell (RBC) antigen is an important concern in myelodysplastic syndromes (MDSs) patients with chronic transfusion, causing potential risk for hemolytic reaction and limited supply of compatible blood. However, there is little data addressing RBC alloimmunization in this patient cohort among the Chinese population. This study aims to evaluate the incidence, specificity of antibodies, and RBC units transfused before antibody formation and its significance in a population of patients consistently receiving RhD-matched RBC units.Methods: We retrospectively reviewed the transfusion and clinical information of all transfused patients with MDS enrolled in our hospital from 2012 to 2022. The cumulative incidence of alloimmunization was analyzed by a Kaplan-Meier plot. Alloimmunization incidence was compared based on different transfused RBC units using the log-rank test.Results: A total of 103 patients with MDS were included in this study; alloantibody formed in 8 (7.8%) patients. Before reaching 32 RBC units, 87.5% of the alloimmunized patients had developed their alloantibodies. All but 1 of the alloantibodies developed were antibodies to Rh antigens. The RBC transfusion intensity and frequency were significantly higher following alloimmunization in the alloimmunized patients (P = .008, P = .008, respectively).Conclusion: The antibodies detected mostly involve the Rh system among MDS patients in China. The alloimmunization tended to occur early prior to reaching 32 RBC units in patients with MDS. Rh antigen matching should be considered early in the patient's transfusion history and completed before receiving 32 RBC units.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141768394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Navigating the conundrum of co-existing autoantibodies and alloantibodies in a case of Evans syndrome. 解决埃文斯综合征病例中同时存在的自身抗体和同种抗体的难题。

Laboratory medicine Pub Date : 2024-07-25 DOI: 10.1093/labmed/lmae051

Pallabi Dash, Nilasish Pani, Jayant Kumar Panda, Smita Mahapatra, Binay Bhusan Sahoo, Tusharkantee Behera

引用次数: 0

Therapeutic plasma exchange for hyperviscosity syndrome in IgA multiple myeloma. 治疗性血浆置换用于治疗 IgA 多发性骨髓瘤的高粘滞性综合征。

Laboratory medicine Pub Date : 2024-07-22 DOI: 10.1093/labmed/lmae054

Nalan Yurtsever, Thomas C Binns, Jeanne E Hendrickson, Christopher A Tormey, Edward S Lee

引用次数: 0

Jerk (d(acceleration)/dt) as an operative variable in pneumatic tube transport (PTT). 作为气动管道输送（PTT）操作变量的抖动（d（加速度）/dt）。

Laboratory medicine Pub Date : 2024-07-18 DOI: 10.1093/labmed/lmae055

Christopher J Mattiello, Douglas F Stickle

{"title":"Jerk (d(acceleration)/dt) as an operative variable in pneumatic tube transport (PTT).","authors":"Christopher J Mattiello, Douglas F Stickle","doi":"10.1093/labmed/lmae055","DOIUrl":"https://doi.org/10.1093/labmed/lmae055","url":null,"abstract":"Background: Jerk, the rate of change of acceleration (d(acceleration)/dt), is a known operative variable in public transportation safety, but this term has never appeared in the literature regarding pneumatic tube transport (PTT) and specimen integrity. We investigated profiles of acceleration and jerk for 2 PTT routes within our hospital system.Methods: Acceleration data were collected for PTT for 2 routes (A, B) using an accelerometer. Acceleration vectors (a) were analyzed in terms of distributions of jerk (da/dt), and distributions of θ, the angle between successive acceleration vectors.Results: Routes A and B had transit times of approximately 300 s. Acceleration vectors (a) ranged in magnitude from 0 to 8 g. For B, a > 1.2 g comprised 29.0% of results, compared to 13.5% of results for A (ratio = 2.1). Jerk ranged from 0 to 94 g/s. For B, jerk > 0.5 g/s comprised 71.9% of results, compared to 32.5% of results for A (ratio = 2.2). θ ranged from 0 to 180 degrees. For B, θ > 5 degrees comprised 59.3% of results, compared to 26.6% of results for A (ratio = 2.2).Conclusion: Differences in distribution in acceleration, jerk, and θ ran in parallel as variables for comparison between 2 PTT routes. Jerk and θ are likely to be operative variables in effects of PTT.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Frequency of serological markers of rheumatoid arthritis in patients with Hashimoto's thyroiditis. 桥本甲状腺炎患者类风湿关节炎血清学指标的频率。

Laboratory medicine Pub Date : 2024-07-03 DOI: 10.1093/labmed/lmad100

Mariam Ghozzi, Amani Mankai, Sarra Melayah, Fatma Mechi, Zeineb Chedly, Abdelhalim Trabelsi, Ibtissem Ghedira

{"title":"Frequency of serological markers of rheumatoid arthritis in patients with Hashimoto's thyroiditis.","authors":"Mariam Ghozzi, Amani Mankai, Sarra Melayah, Fatma Mechi, Zeineb Chedly, Abdelhalim Trabelsi, Ibtissem Ghedira","doi":"10.1093/labmed/lmad100","DOIUrl":"10.1093/labmed/lmad100","url":null,"abstract":"Background: Hashimoto's thyroiditis (HT) is an autoimmune disease that is frequently associated with other autoimmune conditions.Objective: To perform serological screening for rheumatoid arthritis (RA) in patients with HT.Methods: Our study included 88 consecutive serum specimens of patients with confirmed HT and 88 sex- and age-matched healthy subjects. All study participants were tested for anti-cyclic citrullinated peptides antibodies (CCP-Ab) and rheumatoid factor (RF). CCP-Ab and RF were performed using ELISA commercial kits. Statistical analysis was conducted using Epi Info, version 3.Results: Out of 88 patients with HT, 15 (17.0%) had CCP-Ab or RF. The frequency of serological markers of RA was significantly higher in patients than in control individuals (17.0% vs 4.5%; P = .007). RF was more frequent in patients than in the control group, and the difference was statistically significant (13.6% vs 3.4%; P = .01). Isolated RF-IgM was absent in all controls and present in 6 patients with HT (6.8% vs 0%; P = .02). Out of 14 male patients, 3 (21.4%) had antibodies of RA. There was no significant difference in age between patients with CCP-Ab or RF and those without.Conclusion: A high frequency of serological markers of RA was highlighted in patients with HT.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"433-438"},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138465222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Familial nonmedullary thyroid cancer: a case series in Iranian patients with a meta-review of case series. 家族性非髓样甲状腺癌:伊朗患者的病例系列与病例系列的荟萃回顾。

Laboratory medicine Pub Date : 2024-07-03 DOI: 10.1093/labmed/lmad098

Zohreh Mohammadi Zaniani, Mehrdad Zeinalian, Mohammad Amin Tabatabaiefar

{"title":"Familial nonmedullary thyroid cancer: a case series in Iranian patients with a meta-review of case series.","authors":"Zohreh Mohammadi Zaniani, Mehrdad Zeinalian, Mohammad Amin Tabatabaiefar","doi":"10.1093/labmed/lmad098","DOIUrl":"10.1093/labmed/lmad098","url":null,"abstract":"Background: Nonmedullary thyroid cancer (NMTC) comprises approximately 90% of all thyroid cancers, and about 3% to 9% of NMTC cases have a familial origin. Familial NMTC (FNMTC) in the absence of a documented familial cancer syndrome such as Cowden syndrome is characterized by the occurrence of thyroid cancer of follicular cell origin in 2 or more first-degree relatives.Methods: Whole-exome sequencing (WES) was used to identify pathogenic genetic variants in 2 Persian families with FNMTC. The purpose of this work is to assess the pathogenic status of these variants as well as the cosegregation status of the variants observed in the examined families.Results: By analyzing WES data in the first family, SRGAP1: NM_020762: exon16: c.C1849T was identified as a pathogenic variant. This variant was confirmed by Sanger sequencing. In the second family, the variant FOXE1: NM_004473: exon1: c.531_532insCGCGA was identified but was not confirmed by Sanger sequencing.Conclusion: Based on the data, SRGAP1 can be a potential candidate gene for susceptibility to FNMTC in the first family. However, additional analyses like whole genome sequencing and copy number variations are required to ascertain the disease status in second family.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"506-516"},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138441907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical management of a patient following a granulocyte transfusion from a donor positive for COVID-19. 从 COVID-19 阳性供体处输注粒细胞后患者的临床治疗。

Laboratory medicine Pub Date : 2024-07-03 DOI: 10.1093/labmed/lmad118

Jennifer S Woo, Lefan Zhuang, Ryan Jackson, Shirong Wang, Vaibhav Agrawal, Amanda Blackmon, Hoda Pourhassan, Shan Yuan

引用次数: 0