Laboratory medicine最新文献_第9页

Correction to: The utility of an algorithm based on procalcitonin monitoring in patients with sepsis. 更正：基于脓毒症患者降钙素原监测的算法的实用性。

Laboratory medicine Pub Date : 2025-01-06 DOI: 10.1093/labmed/lmae093

引用次数: 0

Evaluation of polypropylene CSF low-bind collection tubes for trace metal contamination. 评估聚丙烯 CSF 低粘合剂收集管的痕量金属污染。

Laboratory medicine Pub Date : 2025-01-06 DOI: 10.1093/labmed/lmae067

Anna C Bitzer, Paul J Jannetto, Joshua A Bornhorst

{"title":"Evaluation of polypropylene CSF low-bind collection tubes for trace metal contamination.","authors":"Anna C Bitzer, Paul J Jannetto, Joshua A Bornhorst","doi":"10.1093/labmed/lmae067","DOIUrl":"10.1093/labmed/lmae067","url":null,"abstract":"Background: Due to the ability of metal ions to cross the blood-brain barrier, there has been interest in analyzing cerebrospinal fluid (CSF) for trace element concentrations to investigate possible correlations with neurodegenerative diseases. In this study, Sarstedt polypropylene CSF collection tubes were analyzed to determine the contamination levels of aluminum, titanium, chromium, manganese, cobalt, nickel, molybdenum, gadolinium, vanadium, arsenic, cadmium, mercury, lead, thallium, selenium, copper, zinc, and iron.Methods: Sarstedt polypropylene CSF collection tubes from 2 separate lots (n = 10 per lot) were filled with a 2 mL aliquot of a CSF pool with known element concentrations. After 24 hours of leaching at room temperature, all 18 elements were analyzed via inductively coupled plasma mass spectrometry (ICP-MS). Results were subtracted from the initial pool concentration to determine contamination levels.Results: No detectable contamination above the assay limit of detection was found in 11 analytes. Molybdenum and selenium contamination was measured in all tubes, and aluminum, titanium, manganese, thallium, and zinc had minimal levels of sporadic detectable contamination in 25% or fewer of the tubes tested.Conclusions: Sarstedt polypropylene CSF tubes are an acceptable collection tube for the analysis of most assessed metals in CSF.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"64-69"},"PeriodicalIF":0.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141972502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Case report - Hyperthermia and nuclear hypersegmentation of blood cells. 病例报告-热疗和核细胞超分割。

Laboratory medicine Pub Date : 2025-01-03 DOI: 10.1093/labmed/lmae105

C Rivière, S Vimeux, S Laurens, L Deluche, P Y Juvin, A Sanson, D Metsu

引用次数: 0

CALR frameshift mutation detection in myeloproliferative neoplasms by microfluidic chip analysis. 应用微流控芯片检测骨髓增殖性肿瘤CALR移码突变。

Laboratory medicine Pub Date : 2024-12-24 DOI: 10.1093/labmed/lmae096

Michael P Greenwood, Keith M Newton, Kristi L Pepper, Heather L Hendrickson, Randall J Olsen, Jessica S Thomas

{"title":"CALR frameshift mutation detection in myeloproliferative neoplasms by microfluidic chip analysis.","authors":"Michael P Greenwood, Keith M Newton, Kristi L Pepper, Heather L Hendrickson, Randall J Olsen, Jessica S Thomas","doi":"10.1093/labmed/lmae096","DOIUrl":"https://doi.org/10.1093/labmed/lmae096","url":null,"abstract":"Background: CALR mutation analysis is routinely used to diagnose BCR/ABL1-negative myeloproliferative neoplasms. The 2 most common CALR mutations are a 52-base pair (bp) deletion and a 5-bp insertion, which account for approximately 85% of cases.Methods: To evaluate our new microfluidic chip assay, we tested CALR mutant and wild-type specimens that were previously analyzed using conventional methods at a reference laboratory. Samples included EDTA-anticoagulated peripheral blood and bone marrow specimens, air dried bone marrow aspirate smears, and formalin-fixed, paraffin-embedded bone marrow sections. CALR exon 9 was PCR amplified using 2 previously published primer pairs and a third unique primer pair designed for our new assay. Amplicons were sized using microfluidic chip analysis.Results: Concordance with the reference method was 100% (42/42). Intra-run and inter-run reproducibility were also 100% (3/3 and 3/3, respectively). The limit of detection was confirmed to be 6% mutant alleles.Conclusion: We determined that the microfluidic chip assay to detect CALR exon 9 mutations was acceptable for clinical use. Compared with the conventional method, the microfluidic analysis assay benefits from a streamlined workflow, faster turnaround, and a smaller instrument footprint.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple HPLC method for the determination of plasma progesterone levels in the third trimester of human pregnancy. 一种测定人妊娠晚期血浆黄体酮水平的简便高效液相色谱法。

Laboratory medicine Pub Date : 2024-12-19 DOI: 10.1093/labmed/lmae098

Mevlut Albayrak, Yucel Kadioglu, Fatma Demirkaya-Miloglu, Bunyamin Borekci

{"title":"A simple HPLC method for the determination of plasma progesterone levels in the third trimester of human pregnancy.","authors":"Mevlut Albayrak, Yucel Kadioglu, Fatma Demirkaya-Miloglu, Bunyamin Borekci","doi":"10.1093/labmed/lmae098","DOIUrl":"https://doi.org/10.1093/labmed/lmae098","url":null,"abstract":"Progesterone is a steroid hormone primarily associated with pregnancy. A simple, rapid, and reliable high-performance liquid chromatography (HPLC) method has been developed and validated for the quantification of progesterone in human plasma. The method consists of a simple liquid-liquid extraction of progesterone and internal standard (estriol) from human plasma using a mixture of hexane and diethyl ether. The chromatographic determination of progesterone was performed using an acetonitrile-water (70:30, v/v) mobile phase with a C18 reversed-phase column. The method achieved an extraction recovery of greater than 96.4% from spiked plasma samples. Intra- and inter-day precision were generally acceptable, with relative SD% less than ≤6.60% and accuracy (relative error %) better than 3.64%. The developed and validated method was used to successfully quantify progesterone levels in plasma samples collected from women during the third trimester of pregnancy. Furthermore, a statistical comparison was conducted between progesterone concentrations in plasma samples obtained from 2 groups of pregnant women: group 1 (n = 9) at 30-35 weeks and group 2 (n = 9) at 36-41 weeks. The developed and validated HPLC method described in this study enables the successful determination of progesterone in human plasma, offering advantages such as shorter analysis time, simplicity, cost-effectiveness, and potential routine use during pregnancy.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cracking the code of aldosterone synthase deficiency: bridging genetics and biochemistry: a case report. 破解醛固酮合成酶缺乏症的密码：连接遗传学和生物化学：一例报告。

Laboratory medicine Pub Date : 2024-12-19 DOI: 10.1093/labmed/lmae102

Lekha Priyadharshini Kamarajan, Mala Mahto, Sushil Kumar, Pradeep Kumar

{"title":"Cracking the code of aldosterone synthase deficiency: bridging genetics and biochemistry: a case report.","authors":"Lekha Priyadharshini Kamarajan, Mala Mahto, Sushil Kumar, Pradeep Kumar","doi":"10.1093/labmed/lmae102","DOIUrl":"https://doi.org/10.1093/labmed/lmae102","url":null,"abstract":"Objective: Aldosterone synthase deficiency (ASD) is a rare autosomal recessive inherited disease with an overall clinical phenotype of failure to thrive, vomiting, severe dehydration, hyperkalemia, and hyponatremia. Mutations in the CYP11B2 gene encoding AS are responsible for the occurrence of ASD. Defects in CYP11B2 gene have only been reported in a limited number of cases worldwide. Due to this potential life-threatening risk, a comprehensive hormonal investigation followed by genetic confirmation is essential for the clinical management of offspring.Methods: We report a case of a newborn who was found to have persistent hyponatremia, hyperkalemia, low aldosterone level, raised renin levels, normal cortisol, and normal 17 hydroxyprogesterone level, suggesting the diagnosis of isolated ASD.Results: Genetic report was suggestive of isolated ASD caused by a novel base pair deletion in exon 3, homozygous CYP11B2 variant (chr8:g.142915123_142915125del; depth: 124x d) (p.Lys175del; ENST00000323110.2). After initial steps of rehydration and salt restoration, the child was started on oral tablet fludrocortisone. The child responded well and showed a good gain in growth and development.Discussion: We elaborate on the biochemical and genetic work-up performed and describe potential pitfalls in CYP11B2 sequencing due to its homology to CYP11B1.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurturin gene IVSI-663 polymorphism but not RET variants is associated with increased risk for breast cancer. Neurturin 基因 IVSI-663 多态性而非 RET 变异与乳腺癌风险增加有关。

Laboratory medicine Pub Date : 2024-12-13 DOI: 10.1093/labmed/lmae097

Tuba Taşkan, Farshad Noori, Osman Kurukahvecioğlu, Niyazi Karaman, Aymelek Gönenç

{"title":"Neurturin gene IVSI-663 polymorphism but not RET variants is associated with increased risk for breast cancer.","authors":"Tuba Taşkan, Farshad Noori, Osman Kurukahvecioğlu, Niyazi Karaman, Aymelek Gönenç","doi":"10.1093/labmed/lmae097","DOIUrl":"https://doi.org/10.1093/labmed/lmae097","url":null,"abstract":"Background: Gene polymorphisms of rearranged during transfection (RET) and its ligand neurturin (NRTN) are one of the focus of studies in the investigation of cancer pathogenesis, invasion, and metastasis. In this study, we aimed to examine the possible risk of breast cancer between RET G691S, L769L, S904S, and NRTN IVSI-663 polymorphisms and to evaluate serum NRTN, brain-derived neurotrophic factor (BDNF), matrix metalloproteinase (MMP)-2, MMP-9, and focal adhesion kinase (FAK) levels.Methods: The study consists of 110 breast cancer patients and 110 controls. Polymorphisms were detected by the polymerase chain reaction method from study groups whole blood.Results: The NRTN IVSI-663 polymorphism in G allele has been found to be 1.54 fold increased the risk of breast cancer, however AA genotype has been found 0.43 fold decreased the risk of breast cancer (P < .05, P < .05, respectively). Study groups showed a similar profile for RET G691S, L769L, S904S allele frequencies and genotype distributions (P > .05). In the patient group, significant increase in serum NRTN and FAK levels and decrease in MMP-2 and MMP-9 levels were found (P < .05, P < .05, P < .05, P < .05, respectively).Discussion: In summary that increased breast cancer risk with the G allele in NRTN gene IVSI-663 polymorphism, as well as the increased serum NRTN and FAK levels, will contribute to the diagnosis, prognosis and determination of new treatment strategies.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of organism recovery and staining properties for 3 methods of Gram stain preparation. 比较三种革兰氏染色制备方法的菌体回收率和染色性能。

Laboratory medicine Pub Date : 2024-12-04 DOI: 10.1093/labmed/lmae095

Audrey Pisahl, Martha Timmons, Sheila Criswell

{"title":"Comparison of organism recovery and staining properties for 3 methods of Gram stain preparation.","authors":"Audrey Pisahl, Martha Timmons, Sheila Criswell","doi":"10.1093/labmed/lmae095","DOIUrl":"https://doi.org/10.1093/labmed/lmae095","url":null,"abstract":"Background: One possible way to improve accuracy of blood culture Gram stain analyses is increasing the concentration of organisms on the slide prepared from the blood culture broth.Methods: From each positive blood culture bottle, 1 direct smear, a 1-drop concentrated preparation, and 1 cytospin/cytofuge preparation were Gram stained and evaluated. There were 2 evaluators who ranked the 3 preparations from most to fewest organisms seen. Each preparation was also scored as acceptable or unacceptable for both organism and background stain quality.Results: The 1-drop slide exhibited the highest concentration of organisms compared with both the cytospin and direct smear but presented frequent difficulties with interpretation of Gram staining acceptability with both the background and the organisms. Although cytospin preparations are known to concentrate liquid specimens, the current study found no enrichment of microorganisms over the direct smear preparation. The cytospin was, however, advantageous for enhancing organism morphology by creating a monolayer of elements and reducing background artifacts.Conclusion: The 1-drop preparation is simple, inexpensive, and effective at increasing organism concentration for analysis, making it a good option when processing culture bottles with low organism loads, but more investigation into methods of improving stain quality is necessary.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142831563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of decreased granules in neutrophils by automated hematology analyzers XR-1000 and UniCel DxH 800. 用自动血液分析仪 XR-1000 和 UniCel DxH 800 检测中性粒细胞中减少的颗粒。

Laboratory medicine Pub Date : 2024-11-04 DOI: 10.1093/labmed/lmae047

Yosuke Kato, Daisuke Sakamoto, Hiroaki Ohnishi, Tomohiko Taki

{"title":"Detection of decreased granules in neutrophils by automated hematology analyzers XR-1000 and UniCel DxH 800.","authors":"Yosuke Kato, Daisuke Sakamoto, Hiroaki Ohnishi, Tomohiko Taki","doi":"10.1093/labmed/lmae047","DOIUrl":"10.1093/labmed/lmae047","url":null,"abstract":"Objective: This study aimed to investigate the utility of neutrophil-related cell population data obtained by automated hematology analyzers in assessing myelodysplastic syndrome cases with decreased granules in neutrophils.Methods: A total of 108 subjects were classified into normal granule (n = 35), hypogranulation (n = 37), or hypergranulation (n = 36) groups. Neutrophil cell area and granule area were measured by ImageJ. All samples were analyzed on the XR-1000 and UniCel DxH 800, and neutrophil-related parameters were compared among the 3 groups.Results: Neutrophil cell area and the ratio of the granular area showed significant differences among the 3 groups; they were the highest in the hypergranulation group and lowest in the hypogranulation group. XR-1000 data showed significant differences in NE-SFL and NE-FSC among the 3 groups (P < .0001). NE-SFL and NE-FSC discriminated most accurately hypogranulation group against other groups. UniCel DxH 800 data showed significant differences in MN-V-NE, MN-MALS-N, MN-UMALS-NE, SD-UMALS-NE (P <.01), MN-LMALS-NE, and SD-LMALS-NE (P <.05) among the 3 groups. The combination of SD-V-NE and SD-LMALS-NE discriminated most accurately the hypogranulation group against the other groups.Conclusion: NE-SFL and NE-FSC and the combination of SD-V-NE and SD-LMALS-NE are useful in detecting cases with decreased granules in neutrophils.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"768-775"},"PeriodicalIF":0.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute hemolytic transfusion reaction caused by anti-M antibodies: a case report and literature review. 抗 M 抗体引起的急性溶血性输血反应：病例报告和文献综述。

Laboratory medicine Pub Date : 2024-11-04 DOI: 10.1093/labmed/lmae038

Yanjing He, Yang Li, Qiushi Wang

{"title":"Acute hemolytic transfusion reaction caused by anti-M antibodies: a case report and literature review.","authors":"Yanjing He, Yang Li, Qiushi Wang","doi":"10.1093/labmed/lmae038","DOIUrl":"10.1093/labmed/lmae038","url":null,"abstract":"Objective: We report a rare case of acute hemolytic reactions caused by immunoglobulin (Ig)M anti-M antibody and present a literature review.Case report: A 61-year-old male patient who underwent blood transfusion developed fever, chills, soy sauce-colored urine, and changes in laboratory test results, including persistently decreased hemoglobin levels, neutrophilia, elevated lactate dehydrogenase level, acute kidney injury, mild acute liver injury, and activation of the coagulation system, indicating acute hemolytic transfusion reaction (AHTR). Antibody screening and major crossmatching results indicated weak positive at 37°C for both posttransfusion and pretransfusion sample. Subsequent serological examinations indicated the presence of IgM anti-M antibodies in plasma but the direct antiglobulin and elution tests were negative. Antibody hemolytic activity assay confirmed AHTR caused by anti-M. The transfused red blood cells were MM and the patient is NN. These signs and symptoms disappeared rapidly and required no additional interventions before discharge.Conclusion: The accurate diagnosis of anti-M antibody-mediated acute hemolysis is essential for guiding treatment decisions.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"795-801"},"PeriodicalIF":0.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141285758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0