Laboratory medicine最新文献_第10页

ASCP Board of Certification Survey of Medical Laboratory Science education 2023: faculty. ASCP医学检验科学教育认证委员会调查2023：教员。

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Laboratory medicine Pub Date : 2026-01-07 DOI: 10.1093/labmed/lmaf073

Dana Duzan, Karen Fong, Vicki S Freeman, Nancy Goodyear, Teresa S Nadder, Alexa Siddon, Amy Spiczka, Teresa Taff, Patricia Tanabe

{"title":"ASCP Board of Certification Survey of Medical Laboratory Science education 2023: faculty.","authors":"Dana Duzan, Karen Fong, Vicki S Freeman, Nancy Goodyear, Teresa S Nadder, Alexa Siddon, Amy Spiczka, Teresa Taff, Patricia Tanabe","doi":"10.1093/labmed/lmaf073","DOIUrl":"10.1093/labmed/lmaf073","url":null,"abstract":"Introduction: The American Society for Clinical Pathology Board of Certification Research and Development Committee has undertaken regular surveys of Medical Laboratory Science (MLS) education programs to gather information to support MLS program directors and faculty in educating students.Methods: The survey was sent out to MLS program directors and faculty who answered questions related to demographics, education level and rank, certification patterns, experience, responsibilities, salaries, and retirement.Results: Results were mainly analyzed by the respondents' titles. The survey data reveal a newer cohort of program directors and faculty, which affected data such as retirement and experience. Hospital program directors' and faculty's salaries have increased at a similar rate to inflation since 2020, but salaries for university program directors and faculty have increased at a much slower rate than inflation within the same time frame.Discussion: Results of previous surveys in 2019 and 2022 are also reported. At the time the 2023 survey was conducted, laboratory professional training programs were beginning to return to the \"new\" normal following the COVID-19 pandemic restrictions. The results of this survey and those of subsequent years will shed light on the changes in the MLS education field and the impact of the pandemic on program directors and faculty.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145822613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of trimester-specific reference intervals for thyroid hormones based on real-world data using a maximum likelihood method. 基于真实世界数据，使用最大似然法开发甲状腺激素的妊娠特异性参考区间。

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Laboratory medicine Pub Date : 2026-01-07 DOI: 10.1093/labmed/lmaf053

Xiaoyan Chen, Yinrui Zou, Yunxia Chu, Zhaoqi Gu, Xiangrong Yang, Yuqiang Huang, Zhaoxi Wang

{"title":"Development of trimester-specific reference intervals for thyroid hormones based on real-world data using a maximum likelihood method.","authors":"Xiaoyan Chen, Yinrui Zou, Yunxia Chu, Zhaoqi Gu, Xiangrong Yang, Yuqiang Huang, Zhaoxi Wang","doi":"10.1093/labmed/lmaf053","DOIUrl":"10.1093/labmed/lmaf053","url":null,"abstract":"Introduction: We sought to establish trimester-specific reference intervals of thyroid-stimulating hormone (TSH) and free thyroxine (FT4) using real-world data.Methods: Deidentified data for FT4, TSH, and anti-thyroid peroxidase antibody (TPO-Ab) associated with pregnancies from July 1, 2014, to December 31, 2019, were extracted from the institutions' medical records. After data cleaning, trimester-specific reference intervals were established using the maximum likelihood method in TPO-Ab-negative pregnancies.Results: We included 55 323 records after data cleaning. Reference intervals for TSH and FT4 built using the maximum likelihood method in the first, second, and third trimesters were 0.40 to 4.09, 0.57 to 4.04, 0.73 to 4.07 mIU/L and 12.2 to 20.5, 10.2 to 18.2, and 9.0 to 15.5 pmol/L, respectively. Compared with reference intervals from the Guidelines of the American Thyroid Association for the Diagnosis and Management of Thyroid Disease During Pregnancy and the Postpartum (2nd edition), the maximum likelihood method-calculated reference intervals of FT4 were comparable with guideline-suggested reference intervals, with the first trimester reference intervals slightly lower and the second trimester reference intervals showing narrower limits. For TSH, the maximum likelihood method-calculated reference intervals were narrower than guideline-suggested reference intervals.Discussion: Trimester-specific reference intervals of TSH and FT4 for pregnancies were established using the maximum likelihood method. Compared with guideline-suggested reference intervals, no clinically significant discrepancies in FT4 and narrower limits in TSH were observed.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12822496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145498142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An atypical finding on serum immunofixation: a case report. 血清免疫固定的不典型发现：1例报告。

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Laboratory medicine Pub Date : 2026-01-07 DOI: 10.1093/labmed/lmaf060

Ameerah Davids, David F Keren, Annalise E Zemlin, Fatima B Fazel, David L Murray, Ernest Musekwa, Marizna Korf

{"title":"An atypical finding on serum immunofixation: a case report.","authors":"Ameerah Davids, David F Keren, Annalise E Zemlin, Fatima B Fazel, David L Murray, Ernest Musekwa, Marizna Korf","doi":"10.1093/labmed/lmaf060","DOIUrl":"10.1093/labmed/lmaf060","url":null,"abstract":"Introduction: Multiple myeloma (MM) is characterized by the abnormal proliferation of plasma cells, resulting in the overproduction of distinctive monoclonal proteins (M-protein). Suspected MM necessitates screening for M-protein through a combination of serum protein electrophoresis, serum immunofixation (SIFE), and serum free light chain (SFLC) determination. An M-protein appears as a relatively restricted band on agarose gel, where migration in ɑ-2 is rare.Methods: A 55-year-old man with pulmonary tuberculosis presented with severe lower back pain. On examination, he appeared chronically ill, with conjunctival pallor. X-rays revealed vertebral compression fractures. The full blood count confirmed anemia; however, serum calcium and creatinine levels did not meet myeloma-defining event criteria.Results: The serum protein electrophoresis revealed hypogammaglobulinemia, with the SIFE demonstrating unusual unrestricted κ staining in the ɑ-2 region. A markedly elevated κ SFLC and κ:λ ratio were found. Bone marrow examination demonstrated approximately 90% plasmacytosis. Urine immunofixation revealed a small, restricted κ band disproportionate to the κ SFLC. Notably, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identified only polyclonal κ SFLC.Discussion: Given the absence of a discernible M-protein on SIFE, a small κ restriction on urine immunofixation, and a polyclonal increase in κ SFLCs, the patient's condition is being managed as an oligosecretory MM.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12822495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete phenotyping of the pathogenic genotype of the NTRK1 gene in early childhood. 儿童早期NTRK1致病基因型的完全表型分析。

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Laboratory medicine Pub Date : 2026-01-07 DOI: 10.1093/labmed/lmaf085

Antonio Sierra-Rivera, Altea Esteve-Martínez, Virginia Ballesteros-Cogollos, Xavi Aparisi-Domingo, Elena Montesinos-Sanchís, Gustavo Fábregat-Cid, Carmen Llena-Puy, Raquel Rodríguez-López

{"title":"Complete phenotyping of the pathogenic genotype of the NTRK1 gene in early childhood.","authors":"Antonio Sierra-Rivera, Altea Esteve-Martínez, Virginia Ballesteros-Cogollos, Xavi Aparisi-Domingo, Elena Montesinos-Sanchís, Gustavo Fábregat-Cid, Carmen Llena-Puy, Raquel Rodríguez-López","doi":"10.1093/labmed/lmaf085","DOIUrl":"10.1093/labmed/lmaf085","url":null,"abstract":"Introduction: Congenital insensitivity to pain with anhidrosis (CIPA), caused by biallelic pathogenic variants in the NTRK1 gene, is a rare disorder characterized by the loss of pain sensation, anhidrosis, and impaired temperature regulation.Methods and results: We present a clinical case of a 2-year-old male Chinese patient, compound heterozygous for 2 NTRK1 pathogenic variants-c.851-33T>A and c.1805G>A-confirmed by clinical exome sequencing. The patient exhibited classic CIPA symptoms, including recurrent fever episodes, xerotic skin, sparce hair, abnormality of the dentition, and anhidrosis. The child also displayed preserved fine touch but lacked pain response to stimuli, emphasizing the clinical challenges posed by pain insensitivity, including an increased risk of injury. Multidisciplinary management approach involving dermatology, pediatrics, pediatric neurology, pediatric dentistry, pain management, and genetics was coordinated.Discussion: This case reinforces the importance of early diagnosis and lifelong palliative care to prevent complications and manage symptoms. In addition, this report highlights the higher prevalence of CIPA in Asian populations and the need for ongoing research to explore potential therapeutic interventions targeting pain perception and neurodevelopmental pathways.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145764219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can cMyc challenge cTn? cMyc能挑战cTn吗？

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Laboratory medicine Pub Date : 2026-01-07 DOI: 10.1093/labmed/lmaf059

Qing Li, Chu-Jun Yang, Rui Feng, Xiao-Hui Liu, Zhen-Lu Zhang

{"title":"Can cMyc challenge cTn?","authors":"Qing Li, Chu-Jun Yang, Rui Feng, Xiao-Hui Liu, Zhen-Lu Zhang","doi":"10.1093/labmed/lmaf059","DOIUrl":"10.1093/labmed/lmaf059","url":null,"abstract":"Introduction: The early diagnosis of acute coronary syndrome remains challenging, with high-sensitivity cardiac troponin (hs-cTn) exhibiting limitations in the first 3 hours after symptom onset. Cardiac myosin-binding protein C (cMyc) shows promise as an earlier, more specific biomarker.Methods: Comparative analyses of cMyc vs hs-cTn in multicenter studies (eg, the Kaier trial, n = 1954) and the integration of this testing into 0/1-hour algorithms were assessed. Applications in myocardial infarction subtyping, cardiac surgery, heart failure, and prehospital settings were also examined.Results: Cardiac myosin-binding protein appears in circulation within 30 minutes of ischemia and peaks earlier (6 times faster than hs-cTnT). In non-ST-segment elevation myocardial infarction, cMyc combined with hs-cTn increased rule-out rates from 10.9% to 41.9% (P < .001). Its cardiac-specific N-terminal fragment (C0C1f) minimizes false positives, and point-of-care testing feasibility (70-minute turnaround) was demonstrated. Cardiac myosin-binding protein also showed prognostic value in heart failure and cardiac surgery.Discussion: Cardiac myosin-binding protein demonstrates superior early diagnostic capability for acute coronary syndrome compared with hs-cTn, with potential to transform current diagnostic paradigms.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unsupervised k-means clustering identified routine clinical biomarkers for classifying chronic kidney disease in patients with chronic heart failure. 无监督k-均值聚类确定了慢性心力衰竭患者慢性肾脏疾病分类的常规临床生物标志物。

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Laboratory medicine Pub Date : 2026-01-07 DOI: 10.1093/labmed/lmaf080

Liang Gong, Zhanglin Hu, Xiuying Lin, Wei Chen

{"title":"Unsupervised k-means clustering identified routine clinical biomarkers for classifying chronic kidney disease in patients with chronic heart failure.","authors":"Liang Gong, Zhanglin Hu, Xiuying Lin, Wei Chen","doi":"10.1093/labmed/lmaf080","DOIUrl":"10.1093/labmed/lmaf080","url":null,"abstract":"Introduction: We sought to identify a diagnostic panel based on routine clinical biomarkers that can distinguish chronic kidney disease (CKD) from non-CKD among patients who have chronic heart failure (HF) and predict changes in kidney function in this patient population.Methods: A total of 432 patients with chronic HF were enrolled at Ningde Municipal Hospital of Ningde Normal University in China. The k-means clustering method was applied to identify a diagnostic panel capable of distinguishing CKD from non-CKD and predicting changes in kidney function after 1 year in patients with chronic HF.Results: The k-means clustering method identified 2 distinct subgroups among patients with chronic HF, demonstrating 71.59% concordance with actual CKD diagnostic labels. Five biomarkers showed a statistically significant difference between identified subgroups: albumin, lymphocyte percentage, hemoglobin, cholesterol, and apolipoprotein A. The 5-biomarker panel can distinguish CKD from non-CKD in patients with chronic HF at an area under the curve (AUC) of 0.81. Furthermore, this panel demonstrated predictive value for kidney function changes, with an AUC of 0.87 for identifying improved kidney function and 0.75 for predicting worsening kidney function.Discussion: Our findings suggested that a panel of routine clinical biomarkers can enhance the detection of CKD in patients with chronic HF and may provide additional prognostic value beyond traditional assessments, such as estimated glomerular filtration rate.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145608125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The variability of cell-derived microparticles and the age of healthy blood donors. 细胞源性微粒的变异与健康献血者的年龄。

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Laboratory medicine Pub Date : 2026-01-07 DOI: 10.1093/labmed/lmaf072

Surada Lerdwana, Duangdao Palasuwan, Attakorn Palasuwan, Egarit Noulsri

{"title":"The variability of cell-derived microparticles and the age of healthy blood donors.","authors":"Surada Lerdwana, Duangdao Palasuwan, Attakorn Palasuwan, Egarit Noulsri","doi":"10.1093/labmed/lmaf072","DOIUrl":"10.1093/labmed/lmaf072","url":null,"abstract":"Introduction: Cell-derived microparticles that promote coagulation can lead to transfusion-related complications. Although age-dependent changes in hemostasis are known, the impact of donor age on microparticle concentration variability remains largely unexplored. We sought to determine microparticle concentrations and investigate their relationship with donor age.Methods: Whole-blood samples were collected from volunteers aged 17 to 60 years using K3EDTA as an anticoagulant. Donors were allocated to 1 of 5 age groups. Flow cytometric analysis and counting beads were used to determine microparticle concentrations and their origins.Results: A cross-sectional study of 394 blood donors revealed a mean (SD) total microparticle count of 25 693 (1578), 26 956 (976), 26 979 (989), 24 886 (987), and 271 331 (1355) particles/µL in blood donors aged 17 to 20, 21 to 30, 31 to 40, 41 to 50, and 51 to 60 years, respectively. Similarly, there were no statistically significant differences in the concentrations of red blood cell (RBC)-derived microparticles, platelet-derived microparticles, or leukocyte-derived microparticles among the donor age groups. Linear regression analysis revealed that the r2 values between the total microparticle, RBC-derived microparticle, platelet-derived microparticle, and leukocyte-derived microparticle concentrations in whole blood and donor age were less than 0.01.Discussion: Our assessment of microparticle concentration across different blood donor age groups revealed age-independent variability in microparticle levels. These findings enhance our understanding of how donor factors influence microparticle values.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145590586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stability study of lactate dehydrogenase activity in serum samples at different temperatures and times according to Spanish Society of Laboratory Medicine recommendations. 根据西班牙检验医学学会的建议，在不同温度和时间下血清样品乳酸脱氢酶活性的稳定性研究。

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Laboratory medicine Pub Date : 2026-01-07 DOI: 10.1093/labmed/lmaf062

Reyes Nicolás de Blas, Miriam Menacho Román, Ignacio Arribas Gómez, Paula Orejas Rodríguez-Marqués, Rubén Gómez Rioja

{"title":"Stability study of lactate dehydrogenase activity in serum samples at different temperatures and times according to Spanish Society of Laboratory Medicine recommendations.","authors":"Reyes Nicolás de Blas, Miriam Menacho Román, Ignacio Arribas Gómez, Paula Orejas Rodríguez-Marqués, Rubén Gómez Rioja","doi":"10.1093/labmed/lmaf062","DOIUrl":"10.1093/labmed/lmaf062","url":null,"abstract":"Introduction: To ensure adequate quality of clinical samples, laboratories must control samples' postanalytical preservation. We checked the stability limit of lactate dehydrogenase (LDH) and confirmed the 72-hour limit the manufacturer proposes, then evaluated sample stability under our working conditions using new recommendations for stability studies.Methods: Leftover outpatient samples were randomly selected. Serum tubes with separator gel were used for primary sample collection. In a preliminary study of samples from 6 patients, we made determinations at baseline, 24, 48, and 72 hours for aliquots stored at room temperature and refrigerated. An extended study in refrigerated samples was performed on 10 samples of leftover serum at baseline and 12, 24, 36, 48, 60, and 72 hours.Results: We found no substantial variation in samples at room temperature. In the extended study, an LDH decrease of 1.5% per day is expected up to 72 hours based on the formula PD% = 0.064 ×time [h].Discussion: The available literature on stability studies is extensive and varies in terms of working methodology. Therefore, laboratories should follow recommendations from standardized and updated documents. We modified manufacturer stability recommendations by reducing the stability of refrigerated LDH samples in our laboratory automation system from 72 hours to 48 hours.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145305108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS-CoV-2 humoral immunity is associated with elevated hemoglobin A1c. SARS-CoV-2体液免疫与糖化血红蛋白升高有关。

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Laboratory medicine Pub Date : 2026-01-07 DOI: 10.1093/labmed/lmaf036

Darryl E Palmer-Toy, Rui Yan, Komal J Narwaney, Aida Legaspi, Michael Aragones, Jessica A Lam, John M Chang, Cecilia Portugal, Katia J Bruxvoort, Jason M Glanz, Ingrid A Binswanger

{"title":"SARS-CoV-2 humoral immunity is associated with elevated hemoglobin A1c.","authors":"Darryl E Palmer-Toy, Rui Yan, Komal J Narwaney, Aida Legaspi, Michael Aragones, Jessica A Lam, John M Chang, Cecilia Portugal, Katia J Bruxvoort, Jason M Glanz, Ingrid A Binswanger","doi":"10.1093/labmed/lmaf036","DOIUrl":"10.1093/labmed/lmaf036","url":null,"abstract":"Introduction: This prospective study assessed relationships between COVID-19, humoral immunity, and common laboratory test results.Methods: Blood and urine were collected from adults with clinical SARS-CoV-2 test results before August 2021: nucleic acid amplification (NAA) tests and antinucleocapsid protein (αN) serology. Measurements were made of αN and 13 laboratory tests, and results were compared across groups defined by NAA and αN status.Results: Glycated hemoglobin (HbA1c) and platelet counts were clinically significantly higher among individuals who had COVID-19 than in individuals without evidence of COVID-19. Hemoglobin was more abnormal among patients without evidence of COVID-19. Glycated hemoglobin results were also much higher among patients with prior positive NAA and current or prior positive αN results than in individuals with negative αN results. No other statistically significant associations between NAA or αN and abnormal laboratory results were found.Discussion: Elevated HbA1c results were associated with prior positive NAA findings combined with positive αN results but not with negative αN results. Further investigation into the association of dysregulated glycemic control with humoral immunity to natural infection is warranted, given the well-documented increase in incidence in type 1 and type 2 diabetes following COVID-19. Prior positive NAA results were also associated with a few nonspecific abnormalities of the complete blood cell count.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12822492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145380266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the clinical significance of serum anti-PLA2R antibodies in evaluating clinical features and therapeutic efficacy in idiopathic membranous nephropathy. 血清抗pla2r抗体在评价特发性膜性肾病临床特征及疗效中的临床意义分析。

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Laboratory medicine Pub Date : 2026-01-07 DOI: 10.1093/labmed/lmaf086

Hongying Tang, Xian Cai, Lining Guo, Xiaozhen Qi, Kailiang Wu

{"title":"Analysis of the clinical significance of serum anti-PLA2R antibodies in evaluating clinical features and therapeutic efficacy in idiopathic membranous nephropathy.","authors":"Hongying Tang, Xian Cai, Lining Guo, Xiaozhen Qi, Kailiang Wu","doi":"10.1093/labmed/lmaf086","DOIUrl":"10.1093/labmed/lmaf086","url":null,"abstract":"Introduction: This study aimed to evaluate the clinical utility of serum anti-phospholipase A2 receptor (PLA2R) antibodies in assessing clinical features and therapeutic responses in idiopathic membranous nephropathy (IMN).Methods: A retrospective analysis was conducted on 99 patients with IMN admitted to Tianjin Medical University General Hospital between August 2023 and April 2024, stratified into seronegative and seropositive groups. Baseline characteristics, biochemical parameters, anti-PLA2R antibody levels, and 3 treatment strategy outcomes were analyzed. Receiver operating characteristic curve analysis assessed the diagnostic accuracy of anti-PLA2R antibodies.Results: Results revealed statistically significant differences in albumin and urine total protein (U-TP) between the seronegative and seropositive groups (P < .05). Compared with the seropositive group, patients in the seronegative group had a better prognosis. Compared with the tacrolimus plus methylprednisolone and cyclophosphamide plus methylprednisolone treatment regimens, the recovery of microalbuminuria, U-TP, albumin, and anti-PLA2R was greatest after treatment with rituximab, and the therapeutic effect was better. Importantly, among these 4 markers, the change in anti-PLA2R was most substantial. The receiver operating characteristic analysis identified an optimal anti-PLA2R cutoff of 15.53 ng/mL, achieving 76.77% sensitivity, 100% specificity, and an area under the curve of 93.3% (P < .001).Discussion: These findings highlight that rituximab demonstrates substantial clinical value in improving serum albumin levels, reducing U-TP, microalbuminuria, and anti-PLA2R antibody levels in patients with IMN while also underscoring the critical role of anti-PLA2R antibodies in IMN characterization and therapeutic monitoring.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":"57 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146014063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0