Laboratory medicine最新文献_第7页

The mechanisms of resistance, epidemiological characteristics, and molecular evolution of carbapenem-resistant hypervirulent Klebsiella pneumoniae. 耐碳青霉烯类药物的高病毒性肺炎克雷伯氏菌的耐药机制、流行病学特征和分子进化。

Laboratory medicine Pub Date : 2025-07-11 DOI: 10.1093/labmed/lmae110

Qun Wang, Mei-Yi Ye, Chi Hong, Zu-Pin Li, Lei Lin

{"title":"The mechanisms of resistance, epidemiological characteristics, and molecular evolution of carbapenem-resistant hypervirulent Klebsiella pneumoniae.","authors":"Qun Wang, Mei-Yi Ye, Chi Hong, Zu-Pin Li, Lei Lin","doi":"10.1093/labmed/lmae110","DOIUrl":"10.1093/labmed/lmae110","url":null,"abstract":"Introduction: Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) is a highly pathogenic, drug-resistant, and transmissible \"superbug\" that causes infections in hospitals and communities. Because of the lack of effective antimicrobial treatment options, morbidity and mortality from CR-hvKP infections have increased dramatically, and outbreaks and the rapid spread of CR-hvKP in hospitals have become a major global public health challenge.Methods: The mechanisms of molecular evolution in CR-hvKP include the acquisition of a hypervirulent plasmid encoding a virulence gene by carbapenemase-producing K pneumoniae, the horizontal transfer of plasmids carrying carbapenem resistance genes to hvKP, and the acquisition of fusion plasmids carrying both carbapenem resistance genes and hypervirulent genes by classic K pneumoniae. In addition, hvKP can develop a resistance phenotype under antibiotic pressure.Results: CR-hvKP arises through plasmid-mediated convergence of resistance genes and virulence factors. Its multidrug resistance and lethal pathogenicity fuel hospital outbreaks, requiring urgent action to block plasmid transmission and strengthen surveillance to contain the spread of this evolving superbug.Discussion: In this article, we have summarized the carbapenemase resistance mechanism, evolution mechanism, virulence factors, and epidemiology of CR-hvKP. Our aim was to elucidate the molecular evolutionary mechanism of CR-hvKP and provide a reference for curbing the spread of CR-hvKP.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"323-335"},"PeriodicalIF":0.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurturin gene IVSI-663 polymorphism but not RET variants is associated with increased risk for breast cancer. Neurturin 基因 IVSI-663 多态性而非 RET 变异与乳腺癌风险增加有关。

Laboratory medicine Pub Date : 2025-07-11 DOI: 10.1093/labmed/lmae097

Tuba Taşkan, Farshad Noori, Osman Kurukahvecioğlu, Niyazi Karaman, Aymelek Gönenç

{"title":"Neurturin gene IVSI-663 polymorphism but not RET variants is associated with increased risk for breast cancer.","authors":"Tuba Taşkan, Farshad Noori, Osman Kurukahvecioğlu, Niyazi Karaman, Aymelek Gönenç","doi":"10.1093/labmed/lmae097","DOIUrl":"10.1093/labmed/lmae097","url":null,"abstract":"Background: Gene polymorphisms of rearranged during transfection (RET) and its ligand neurturin (NRTN) are one of the focus of studies in the investigation of cancer pathogenesis, invasion, and metastasis. In this study, we aimed to examine the possible risk of breast cancer between RET G691S, L769L, S904S, and NRTN IVSI-663 polymorphisms and to evaluate serum NRTN, brain-derived neurotrophic factor (BDNF), matrix metalloproteinase (MMP)-2, MMP-9, and focal adhesion kinase (FAK) levels.Methods: The study consists of 110 breast cancer patients and 110 controls. Polymorphisms were detected by the polymerase chain reaction method from study groups whole blood.Results: The NRTN IVSI-663 polymorphism in G allele has been found to be 1.54 fold increased the risk of breast cancer, however AA genotype has been found 0.43 fold decreased the risk of breast cancer (P < .05, P < .05, respectively). Study groups showed a similar profile for RET G691S, L769L, S904S allele frequencies and genotype distributions (P > .05). In the patient group, significant increase in serum NRTN and FAK levels and decrease in MMP-2 and MMP-9 levels were found (P < .05, P < .05, P < .05, P < .05, respectively).Discussion: In summary that increased breast cancer risk with the G allele in NRTN gene IVSI-663 polymorphism, as well as the increased serum NRTN and FAK levels, will contribute to the diagnosis, prognosis and determination of new treatment strategies.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"351-359"},"PeriodicalIF":0.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of multiple myositis-specific antibodies: prevalence and clinical significance. 多发性肌炎特异性抗体的检测：患病率及临床意义。

Laboratory medicine Pub Date : 2025-07-11 DOI: 10.1093/labmed/lmae103

Ameni Jerbi, Sahar Karoui, Mouna Snoussi, Sawsan Feki, Chifa Damak, Zouheir Bahloul, Hatem Masmoudi, Sameh Marzouk, Hend Hachcicha

{"title":"Detection of multiple myositis-specific antibodies: prevalence and clinical significance.","authors":"Ameni Jerbi, Sahar Karoui, Mouna Snoussi, Sawsan Feki, Chifa Damak, Zouheir Bahloul, Hatem Masmoudi, Sameh Marzouk, Hend Hachcicha","doi":"10.1093/labmed/lmae103","DOIUrl":"10.1093/labmed/lmae103","url":null,"abstract":"Background: The multipositivity of myositis-specific antibodies (MSAs) is uncommon. Our study aimed to assess the prevalence and the clinical relevance of multiple positive MSAs in routine practice.Methods: A 10 year single-center retrospective study (2015-2024) was conducted reviewing all samples analyzed with the Dot Myositis EUROLINE (Euroimmun Germany). Clinical data corresponding to samples with multiple positive MSAs was reviewed.Results: Among 140 samples positive for at least 1 MSA, 22 patients (15.7%) were positive for at least 2 MSAs on the same sample. The diagnosis of idiopathic inflammatory myopathies (IIMs) was confirmed in only 6 patients (27.2%). The frequency of double-positive MSAs was 100% in IIM patients and 60% in non-IIM patients. No IIM patients and 40% of non-IIM patients (P = .037) had 3 or more positive MSAs. MSA titers were higher in IIM patients than in non-IIM patients (49 vs 32 AU, P = .33). The clinical IIM subtype joined the IIM subtype corresponding to the predominant MSA. We had no mixed features of either IIM subtype or added severe prognosis.Conclusion: Detection of multiple MSAs is uncommon but possible using immunodot techniques. Taking into account MSA number, level of positivity, and clinical data helps in the interpretation of the results.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":"56 4","pages":"367-374"},"PeriodicalIF":0.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144612771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serologic detection of regenerating protein I alpha by time-resolved fluoroimmunoassay and its clinical value in gastric cancer. 用时间分辨荧光免疫测定法检测再生蛋白 I alpha 的血清学特性及其在胃癌中的临床价值。

Laboratory medicine Pub Date : 2025-07-11 DOI: 10.1093/labmed/lmae106

Zhongyi Xiang, Xiaoyan Wang, Akao Zhu, Xindong Chen, Yuan Qin, Xiumei Zhou, Xueqin Zhao, Yigang Wang, Biao Huang, Hongming Fang, Pengfei Liu

{"title":"Serologic detection of regenerating protein I alpha by time-resolved fluoroimmunoassay and its clinical value in gastric cancer.","authors":"Zhongyi Xiang, Xiaoyan Wang, Akao Zhu, Xindong Chen, Yuan Qin, Xiumei Zhou, Xueqin Zhao, Yigang Wang, Biao Huang, Hongming Fang, Pengfei Liu","doi":"10.1093/labmed/lmae106","DOIUrl":"10.1093/labmed/lmae106","url":null,"abstract":"Background: Regenerating protein I alpha (REG Iα) plays a key role in the progression of gastric cancer (GC). However, the clinical application value of serum REG Iα in GC remains largely unknown.Methods: Serum REG Iα levels were analyzed through time-resolved fluoroimmunoassay (TRFIA) in healthy controls (HCs) and patients with benign gastric disease (BGD) and GC.Results: The REG Iα levels of patients with GC were significantly higher than those of HCs and patients with BGD (P < .0001). The REG Iα levels were higher in patients with GC with poor pathological differentiation type; tumor, node, and metastasis stages III-IV; deep tumor invasion (T3-T4); and distant metastasis (P < .05). The diagnostic efficiency of the combined REG Iα, carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA19-9) tests improved compared with that of the single-indicator test for the diagnosis of patients with GC.Conclusion: REG Iα-TRFIA may facilitate the ancillary diagnosis of GC and have a monitoring role for further progression of GC. It can also help elucidate the possible diagnostic role of serum REG Iα as a noninvasive tool.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"375-383"},"PeriodicalIF":0.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142974088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blue-green inclusions in neutrophils and monocytes as a sign of imminent death: an unexpected finding in the peripheral blood smears. 中性粒细胞和单核细胞中的蓝绿色包涵体是即将死亡的标志：外周血涂片的意外发现。

Laboratory medicine Pub Date : 2025-07-09 DOI: 10.1093/labmed/lmaf037

Antonio Sierra-Rivera, María Asunción Elia-Martínez, Ana Comes-Raga, Carmen Hernández-Hernández, Jorge Ferriz-Vivancos, Ángeles Férez-Martí

{"title":"Blue-green inclusions in neutrophils and monocytes as a sign of imminent death: an unexpected finding in the peripheral blood smears.","authors":"Antonio Sierra-Rivera, María Asunción Elia-Martínez, Ana Comes-Raga, Carmen Hernández-Hernández, Jorge Ferriz-Vivancos, Ángeles Férez-Martí","doi":"10.1093/labmed/lmaf037","DOIUrl":"https://doi.org/10.1093/labmed/lmaf037","url":null,"abstract":"Introduction: Blue-green, refractile, and irregularly contoured inclusions found in the cytoplasm of some leukocytes are a rare and underdiagnosed finding. They have been a subject of discussion among professionals regarding their composition and clinical significance. These inclusions are strongly associated with ischemic liver injury in a multitude of clinical contexts.Methods: Patients admitted between March 2021 and March 2022 were included in this study. Medical records, laboratory results, and progression were reviewed in all patients where the presence of these inclusions in peripheral blood smears observed under the optical microscope was reported.Results: The etiologies of the various liver failures that triggered the appearance of these inclusions were highly varied. Alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase levels were highest in these patients. Lactic acidosis was another consistent feature associated with the inclusions. The mortality rate was 84.6%.Discussion: Peripheral blood smear is the only procedure that results in detection of these inclusions. Therefore, the clinical laboratory professional must be aware of the existence of these inclusions and consider notifying the responsible emergency physicians. In contrast, emergency physicians must also be aware of the severity of this finding and take it into account in the overall clinical assessment of critically ill patients.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144602678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific changes in the levels of circulating cell-free mitochondrial DNA in the serum of patients with diabetic microangiopathy. 糖尿病微血管病变患者血清循环无细胞线粒体DNA水平的特异性变化

Laboratory medicine Pub Date : 2025-07-09 DOI: 10.1093/labmed/lmaf034

Shuhan Dong, Helin Wang, Xinchen Zhou, Zhuo Zhang, Xingtong Zhan, Zhuo Jin, Shuhui Yang, Wei Xia, Limei Liu

{"title":"Specific changes in the levels of circulating cell-free mitochondrial DNA in the serum of patients with diabetic microangiopathy.","authors":"Shuhan Dong, Helin Wang, Xinchen Zhou, Zhuo Zhang, Xingtong Zhan, Zhuo Jin, Shuhui Yang, Wei Xia, Limei Liu","doi":"10.1093/labmed/lmaf034","DOIUrl":"https://doi.org/10.1093/labmed/lmaf034","url":null,"abstract":"Introduction: Diabetic microangiopathy (DMAP) is a common chronic complication of diabetes. This study aimed to explore the early diagnostic value of the copy number and methylation level of cell-free mitochondrial DNA in the serum of patients with DMAP.Methods: A total of 40 patients with simple diabetes (diabetes group) and 50 patients with DMAP (40 with diabetic nephropathy [DN group] and 10 with diabetic retinopathy [DR group]) were included in the study. The copy number and methylation levels of cell-free mitochondrial DNA in serum were examined using real-time polymerase chain reaction and high-resolution melting curves.Results: The serum copy number of cytochrome b (Cytb), NADH dehydrogenase subunit 6 (ND6), and displacement loop (DLoop) genes statistically significantly increased in the DN and diabetes groups compared with the healthy control group (P < .001). The high-resolution melting curve results showed that the degree of methylation of ND6 decreased with the progression of DMAP compared with that in the health control group (P < .001). There was a statistically significant increase in the methylation degree of the DLoop genely in both the DN and DR groups compared with both the health control and diabetes groups (P < 0.001).Discussion: The copy number of the Cytb, ND6, and DLoop genes in patients with DMAP differed from that in the healthy control group. Mitochondrial DNA methylation of ND6 and DLoop may be a novel potential biomarker of DMAP.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144602679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of urinary neutrophil gelatinase-associated lipocalin in predicting acute kidney injury in adult patients with diabetic ketoacidosis: a prospective study. 尿中性粒细胞明胶酶相关脂钙蛋白在预测糖尿病酮症酸中毒成年患者急性肾损伤中的作用：一项前瞻性研究。

Laboratory medicine Pub Date : 2025-07-08 DOI: 10.1093/labmed/lmaf027

Naglaa Hamed Fadel, Mamdouh Radwan El Nahas, Basma Badr Eldin Hasan

{"title":"The role of urinary neutrophil gelatinase-associated lipocalin in predicting acute kidney injury in adult patients with diabetic ketoacidosis: a prospective study.","authors":"Naglaa Hamed Fadel, Mamdouh Radwan El Nahas, Basma Badr Eldin Hasan","doi":"10.1093/labmed/lmaf027","DOIUrl":"https://doi.org/10.1093/labmed/lmaf027","url":null,"abstract":"Introduction: Patients with diabetic ketoacidosis are at heightened risk of increased morbidity and death due to acute kidney injury (AKI). Early AKI detection is therefore essential. A new biomarker called urinary neutrophil gelatinase-associated lipocalin (NGAL) has been used to predict early AKI in several diseases. We aimed to determine the predictive role of urinary NGAL for AKI development in adults with diabetic ketoacidosis.Methods: The study included 56 patients with diabetic ketoacidosis classified into 2 groups based on urinary NGAL levels on admission to the intensive care unit (ICU). Group A had high urinary NGAL levels (≥197.5 nmol/L), and group B had urinary NGAL levels below 79.0 ng/mL. The primary outcome was the development of AKI during the ICU stay.Result: No statistically significant differences in serum creatinine, estimated glomerular filtration rate (eGFR), and serum urea nitrogen were observed between the 2 groups upon admission. On the seventh day of admission, eGFR and urine output were lower in group A than in group B. Within groups A and B, 71.1% and 38.9%, respectively, developed AKI. A urinary NGAL cutoff value above 583.75 nmol/L predicted AKI.Discussion: Urinary NGAL at a cutoff value above 583.75 nmol/L on admission has a good predictive value for AKI in patients with diabetic ketoacidosis. Early detection of AKI could improve diabetic ketoacidosis outcomes and decrease the risk of chronic kidney disease in individuals with diabetes.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144593275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KIT amplitude-based multiplex droplet digital polymerase chain reaction outperforms direct sequencing for sensitive KIT D816 genotyping in core binding factor acute myeloid leukemia. 基于KIT幅度的多重液滴数字聚合酶链反应优于直接测序对核心结合因子急性髓系白血病敏感的KIT D816基因分型。

Laboratory medicine Pub Date : 2025-06-25 DOI: 10.1093/labmed/lmaf031

Shumpei Mizuta, Noriko Bandai, Saya Yoshida, Hiroshi Takashima, Yuka Ohashi, Asami Watanabe, Marina Kawano, Takeshi Ueshimo, Kazuhiro Bandai, Kensuke Fujiwara, Naoko Hiranuma, Takahito Kawata, Akira Tamekane, Mitsumasa Watanabe

{"title":"KIT amplitude-based multiplex droplet digital polymerase chain reaction outperforms direct sequencing for sensitive KIT D816 genotyping in core binding factor acute myeloid leukemia.","authors":"Shumpei Mizuta, Noriko Bandai, Saya Yoshida, Hiroshi Takashima, Yuka Ohashi, Asami Watanabe, Marina Kawano, Takeshi Ueshimo, Kazuhiro Bandai, Kensuke Fujiwara, Naoko Hiranuma, Takahito Kawata, Akira Tamekane, Mitsumasa Watanabe","doi":"10.1093/labmed/lmaf031","DOIUrl":"https://doi.org/10.1093/labmed/lmaf031","url":null,"abstract":"Introduction: The prognosis of acute myeloid leukemia is stratified by genetic abnormalities; however, the detection sensitivity varies by method. The KIT D816 mutation is frequently found in core binding factor leukemia and is associated with a poor prognosis. In this study, we aimed to investigate the performance of multiplex droplet digital polymerase chain reaction (ddPCR) for detecting KIT D816 mutations and propose the practical mutation analysis method for clinical laboratory testing.Methods: We evaluated the detection limit of ddPCR using mixed probes for HEX-labeled wild-type and FAM-labeled mutations (D816V, D816Y, and D816H) by analyzing plasmid mixtures containing these sequences. We compared the frequency of KIT mutations detected by direct sequencing and ddPCR in 20 patients with core binding factor leukemia.Results: Multiplex ddPCR successfully discriminated between mutation types based on plot positions on a 2-dimensional map, with a detection limit of 0.1%. The frequency of D816 mutations was 42.5% using ddPCR and 20% using direct sequencing. Most patients with KIT D816 mutation require hematopoietic stem cell transplantation for chimeric gene clearance.Discussion: Amplitude-based multiplex ddPCR efficiently provides sensitive and accurate genotyping of KIT D816 and has potential applications for other genetic abnormalities.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amylase-producing lung adenocarcinoma with an anaplastic lymphoma kinase fusion mutation: a case report. 产生淀粉酶的肺腺癌合并间变性淋巴瘤激酶融合突变1例。

Laboratory medicine Pub Date : 2025-06-25 DOI: 10.1093/labmed/lmaf017

Shuqing Yang, Wenbin Li, Difeng Guo, Chenshi Lin, Jun Xu, Yan Li, Mengru Jian, Meiqi Wang, Cai Zhiqiang

{"title":"Amylase-producing lung adenocarcinoma with an anaplastic lymphoma kinase fusion mutation: a case report.","authors":"Shuqing Yang, Wenbin Li, Difeng Guo, Chenshi Lin, Jun Xu, Yan Li, Mengru Jian, Meiqi Wang, Cai Zhiqiang","doi":"10.1093/labmed/lmaf017","DOIUrl":"https://doi.org/10.1093/labmed/lmaf017","url":null,"abstract":"Introduction: Hyperamylasemia, typically a sign of digestive system disorders, especially pancreatic issues, has an unexpected link with lung cancer in some patients. This report describes such a case in which hyperamylasemia was associated with ALK fusion mutant lung adenocarcinoma.Methods: We identified the type of tumor through histologic analysis and pulmonary biopsy of the tumor and further identified the type of genetic mutation in the tumor using genetic testing. Subsequently, we continuously monitored the serum amylase levels and tumor size during therapy, and this process presented an analysis on the correlation between hyperamylasemia and lung adenocarcinoma while considering the response to targeted therapy.Results: During the detailed research and observation process, we surprisingly discovered that the serum amylase levels throughout the entire course of therapy exhibited a remarkable parallelism with tumor size and the tumor response to targeted therapy.Discussion: We suggest that serum amylase may be a tumor marker that may lead to a more effective approach to the diagnosis and treatment of related diseases.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyzing the impact of the FISH! Philosophy on postpandemic workplace culture in an academic medical laboratory setting. 分析FISH！学术医学实验室环境下流行病后工作场所文化的哲学。

Laboratory medicine Pub Date : 2025-06-25 DOI: 10.1093/labmed/lmaf010

Lynn A McCain, Melina L Adler, Karen Barron, Gloria Barkley, Chris Distelrath, Julene Pummill, Kamran M Mirza, Jeffrey L Myers

{"title":"Analyzing the impact of the FISH! Philosophy on postpandemic workplace culture in an academic medical laboratory setting.","authors":"Lynn A McCain, Melina L Adler, Karen Barron, Gloria Barkley, Chris Distelrath, Julene Pummill, Kamran M Mirza, Jeffrey L Myers","doi":"10.1093/labmed/lmaf010","DOIUrl":"https://doi.org/10.1093/labmed/lmaf010","url":null,"abstract":"Introduction: The post-COVID-19 work environment has seen widespread employee disengagement and increased turnover rates. To combat this negative cultural shift, the pathology department at a large academic medical center launched a culture-change initiative using the FISH! Philosophy program in fall 2023.Methods: Employees were invited to participate in FISH! Philosophy training and completed a baseline survey before attending (n = 125), a pulse survey at the conclusion of their session (n = 86), and a follow-up survey after 3 to 6 months (n = 53).Results: Employees responded positively to the training (94.1%). Improvement in job satisfaction (4.2% increase) and improved culture (reported by 49%) were noted, along with improvements in other key categories related to interpersonal relationships. Trust, however, saw a statistically significant decline (4.4%). Free-text comments were included in 42% of follow-up surveys, indicating that FISH! practices continue to be implemented in their work areas.Discussion: Although early results are mixed, positive movement has been observed in employee attitudes and behaviors. As more employees, and especially faculty and leadership, are trained, we anticipate that less resistance will be encountered, and lasting culture change will occur.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0