Laboratory medicine最新文献_第3页

Sigma metric and Quality Goal Index assessment of hemoglobin A1c analytical performance in an ambulatory accredited laboratory. 西格玛度量和质量目标指数评估血红蛋白A1c分析性能在一个流动认可的实验室。

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Laboratory medicine Pub Date : 2026-08-04 DOI: 10.1093/labmed/lmag052

Jagdish Chandarana, Kinjal Butani

{"title":"Sigma metric and Quality Goal Index assessment of hemoglobin A1c analytical performance in an ambulatory accredited laboratory.","authors":"Jagdish Chandarana, Kinjal Butani","doi":"10.1093/labmed/lmag052","DOIUrl":"https://doi.org/10.1093/labmed/lmag052","url":null,"abstract":"Introduction: Hemoglobin A1c has a stringent total allowable error (TEa), making high Six Sigma values difficult to attain. In addition, data from ambulatory accredited laboratories are sparse.Methods: Internal quality control (QC) data for 2 levels were collected over 8 months on a single-lot Bio-Rad D-10 high-performance liquid chromatography analyzer. Long-term imprecision was the coefficient of variation (s/x¯) × 100 (CV) of all internal QC results combined, capturing between-month variation. Bias was derived from external quality assessment (EQAS) and assigned QC targets, applied as absolute values. The Sigma metric was (TEa ‒ absolute bias) / CV at TEa levels of 6%, 7%, and 8%; the Quality Goal Index distinguished imprecision-driven from bias-driven performance.Results: Long-term CVs were 3.04% (level 1) and 1.34% (level 2); EQASs were satisfactory throughout. At a TEa of 6% with EQAS-derived bias, Sigma metrics were 1.19 and 2.70 and, with a level-specific QC target bias, 1.46 and 4.22. At the Clinical Laboratory Improvement Amendments 2025 criterion of 8%, the Sigma metrics were 2.11 and 5.71. The level 1 Quality Goal Index (0.52) identified imprecision as predominant.Discussion: Despite satisfactory external proficiency, Sigma metrics fell below 3 at both levels against a 6% TEa and below 2 at the clinically pivotal lower concentration, driven by imprecision rather than bias. No control strategy compensates for this; therefore, we proposed a precision-focused corrective plan that targets between-month instability.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":"57 5","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148803876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Change management and continuous assurance of laboratory information system-based autoverification rules aligned with ISO 15189:2022: a practical framework for clinical laboratories. 符合ISO 15189:2022：临床实验室实用框架的基于实验室信息系统的自动验证规则的变更管理和持续保证。

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Laboratory medicine Pub Date : 2026-08-04 DOI: 10.1093/labmed/lmag060

Zilin Li, Lujuan Cui, Yinzhi Feng, Shiqian Feng

{"title":"Change management and continuous assurance of laboratory information system-based autoverification rules aligned with ISO 15189:2022: a practical framework for clinical laboratories.","authors":"Zilin Li, Lujuan Cui, Yinzhi Feng, Shiqian Feng","doi":"10.1093/labmed/lmag060","DOIUrl":"10.1093/labmed/lmag060","url":null,"abstract":"Introduction: Laboratory information system (LIS)-based autoverification can improve turnaround time, consistency, and workload management, but rules may drift as analytical methods, reagent and calibrator lots, reference ranges, patient case mix, interfaces, and information systems change. This review proposes a standards-anchored framework for governing rules after rule implementation and maintaining patient safety across the rule life cycle.Methods: Requirements from International Organization for Standardization (ISO) 15189:2022, ISO 22367:2026, Clinical and Laboratory Standards Institute (CLSI) AUTO15, archived CLSI AUTO10-A, and China Health Organization standard WS/T 616-2018 were integrated with select literature on autoverification and patient safety. No patient-identifiable data or new experimental data were used.Results: The framework consisted of a life cycle model; a taxonomy of change triggers; a 6-domain low, moderate, and high risk assessment; proportionate verification or revalidation methods; a minimum version control and deployment record; monitoring indicators; and discipline-specific considerations for chemistry, immunoassay, hematology, and coagulation. It also defined reference-adjudicated false-pass and false-hold measures and presented a wholly synthetic potassium example.Discussion: Laboratory information system-based autoverification rules should be managed as controlled, versioned, risk-sensitive clinical decision-support tools through structured change control, risk-proportionate verification or revalidation, and continuous monitoring throughout their life cycle.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":"57 5","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148908481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A protocol for autologous platelet-rich plasma collection using an automated blood cell separator: a retrospective study. 使用自动血细胞分离器收集自体富血小板血浆的方案：回顾性研究。

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Laboratory medicine Pub Date : 2026-08-04 DOI: 10.1093/labmed/lmag047

Lei Shi, Lin Peng, Lisheng Chen, Ying Huang, Bing Wei, Weiting Ke, Zhenglian Luo

{"title":"A protocol for autologous platelet-rich plasma collection using an automated blood cell separator: a retrospective study.","authors":"Lei Shi, Lin Peng, Lisheng Chen, Ying Huang, Bing Wei, Weiting Ke, Zhenglian Luo","doi":"10.1093/labmed/lmag047","DOIUrl":"https://doi.org/10.1093/labmed/lmag047","url":null,"abstract":"Introduction: Automated blood cell separators are commonly used for autologous platelet-rich plasma (PRP) preparation. Although these devices ensure stable platelet concentrations, low contamination, and high reproducibility, unified protocols remain lacking. This study explored a collection protocol that uses real-time visual feedback and dynamic parameter adjustment.Methods: In this single-center retrospective study, we analyzed 35 autologous PRP collections. Visual criteria (pale lemon-yellow appearance, uniform turbidity, absence of macroscopically visible particles) and adjustment rules were developed. Platelet concentration, enrichment factor, and residual red blood cell (RBC) and white blood cell levels were assessed.Results: Median platelet concentration was 1500 × 109/L (IQR, 1031-1618 × 109/L), and the mean (SD) enrichment factor was 6.33 (2.76); 71.43% (25/35) of products fell within a range of 1000 to 1800 × 109/L, consistent with reported 3-fold to 8-fold enrichment. Red blood cell counts ranged from 0 to 0.20 × 1012/L. No macroscopic RBCs were detected, but 37.1% (13/35) of RBCs exceeded a threshold of 0.05 × 1012/L. White blood cell counts ranged from 0 to 1.69 × 109/L, below the leukocyte-poor threshold proposed by Kikuchi et al.Discussion: This study presented a protocol capable of producing PRP within the therapeutic range and achieving effective leukocyte control. Our findings provide a practical strategy for translating subjective experience into objective, trainable procedures. Given the limitations of small sample size and subjective visual criteria, further prospective machine vision validation is warranted.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":"57 5","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148687247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water bath prewarming of urine samples to facilitate automated microscopy and urinalysis. 水浴预热尿液样本，以方便自动显微镜和尿液分析。

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Laboratory medicine Pub Date : 2026-08-04 DOI: 10.1093/labmed/lmag071

Samuel W L Baumgart, Ruvanika Rajapakse, Juliette Holland

{"title":"Water bath prewarming of urine samples to facilitate automated microscopy and urinalysis.","authors":"Samuel W L Baumgart, Ruvanika Rajapakse, Juliette Holland","doi":"10.1093/labmed/lmag071","DOIUrl":"https://doi.org/10.1093/labmed/lmag071","url":null,"abstract":"Introduction: We sought to evaluate the effect of water bath prewarming of macroscopically turbid urine samples to facilitate automated microscopy and biochemical analysis. In addition, we sought to confirm that relevant microscopy and biochemical parameters for urine culture interpretation were not compromised by prewarming.Methods: We analyzed 50 turbid urine samples submitted for urine culture. An aliquot of samples underwent manual and automated analysis before prewarming in a water bath at 80 °C for 50 seconds. Manual and automated analyses were repeated following prewarming.Results: Prewarming facilitated an improvement in the number of acceptable urine samples for automated analysis (from 3/50 [6%] to 46/50 [92%]). All biochemical parameters were concordant. Microscopy results were more than 85% concordant between prewarming and postwarming analysis.Discussion: Our testing demonstrated that brief prewarming in a water bath facilitates automated analysis without compromising relevant microscopy and biochemical parameters that affect the workup and interpretation of urine cultures.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":"57 5","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149043218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving laboratory criteria to reduce unnecessary peripheral blood smear reviews by pathologists. 改进实验室标准，减少病理学家不必要的外周血涂片检查。

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Laboratory medicine Pub Date : 2026-08-04 DOI: 10.1093/labmed/lmag061

Lincoln A Brown, Elizabeth Simonds, Claudio A Mosse

{"title":"Improving laboratory criteria to reduce unnecessary peripheral blood smear reviews by pathologists.","authors":"Lincoln A Brown, Elizabeth Simonds, Claudio A Mosse","doi":"10.1093/labmed/lmag061","DOIUrl":"10.1093/labmed/lmag061","url":null,"abstract":"Introduction: Pathologists frequently perform reviews of peripheral blood smears with limited clinical impact. There is considerable heterogeneity, however, in the laboratory criteria different institutions use to trigger these pathologist blood smear reviews (PBSRs).Methods: We retrospectively reviewed 1782 laboratory-initiated PBSRs performed at our institution to understand the indications for PBSR and their outcomes. Based on our findings, we revised our laboratory's PBSR criteria as part of a quality improvement initiative to reduce the number of unnecessary reviews. All PBSRs were tracked for several months before and after the intervention to assess the impact of our intervention.Results: At baseline, 89% of PBSRs yielded no additional information, and only 6% yielded new information that appeared to be clinically impactful. The PBSR criteria based on abnormal platelet counts or leukopenia contributed to a disproportionate number of noncontributory reviews. Removing these conditions from our criteria led to an increase in the proportion of PBSRs yielding novel information from 7% to 16% and reduced the total number of reviews performed monthly by 17%.Discussion: Removal of low-yield conditions from our laboratory's PBSR criteria led to a reduction in the number of unnecessary reviews performed. Our work demonstrates a practical quality improvement strategy to improve PBSR utility without compromising patient care.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":"57 5","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13585456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148993201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stability of serum calprotectin as an emerging inflammatory biomarker: a five-day study at -20 °C and -80 °C using two analytical methods. 血清钙保护蛋白作为一种新兴炎症生物标志物的稳定性：在-20°C和-80°C下使用两种分析方法进行的为期五天的研究。

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Laboratory medicine Pub Date : 2026-08-04 DOI: 10.1093/labmed/lmag059

Helena Čičak, Željka Šarčević, Andrea Saračević, Lora Dukić, Edvard Galić, Igor Alfirević

{"title":"Stability of serum calprotectin as an emerging inflammatory biomarker: a five-day study at -20 °C and -80 °C using two analytical methods.","authors":"Helena Čičak, Željka Šarčević, Andrea Saračević, Lora Dukić, Edvard Galić, Igor Alfirević","doi":"10.1093/labmed/lmag059","DOIUrl":"https://doi.org/10.1093/labmed/lmag059","url":null,"abstract":"Introduction: Serum calprotectin is an emerging inflammatory biomarker, but data on its stability remain limited. This study aimed to determine the 5-day stability of serum calprotectin stored at 2 temperatures: ‒20 °C and ‒80 °C.Methods: This study followed Checklist for Reporting Stability Studies recommendations using leftover serum samples. Analysis was undertaken using 2 analytical methods on an automated Siemens Atellica Solution analyzer. Twenty samples in ‒20 °C storage were analyzed using fCAL turbo reagent (BÜHLMANN), and another 10 samples in ‒20 °C storage were analyzed using GCAL reagent (Gentian Diagnostics). A further 10 patient samples stored at ‒80 °C were analyzed using fCAL turbo reagent. The maximum permissible difference was defined as total error, which was 56.43% for fCAL turbo reagent and 46.44% for GCAL reagent.Results: The instability equations were y = 7.6695 × time (days) and y = 0.8553 × time (days) for temperatures of ‒20 °C and ‒80 °C, respectively, when calprotectin was determined using fCAL turbo reagent and y = 12.7011 × time (days) at ‒20 °C using GCAL reagent.Discussion: Results from samples stored for a maximum of 5 days at ‒80 °C are more reliable than those stored at ‒20 °C, which may have an impact on clinical interpretation of the results.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":"57 5","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148914615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolution of hemoglobinopathy diagnosis over 30 years in southern Spain: impact of migration and laboratory screening strategies. 西班牙南部30年来血红蛋白病诊断的演变：迁移和实验室筛查策略的影响。

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Laboratory medicine Pub Date : 2026-08-04 DOI: 10.1093/labmed/lmag045

María-Angustias Molina-Arrebola, Alicia Sánchez-Crespo, Ana-María Alarcón-Gallo, Carmen Porrino-Herrera, Lucía Martínez-Carreras, Carlos González-Oller

{"title":"Evolution of hemoglobinopathy diagnosis over 30 years in southern Spain: impact of migration and laboratory screening strategies.","authors":"María-Angustias Molina-Arrebola, Alicia Sánchez-Crespo, Ana-María Alarcón-Gallo, Carmen Porrino-Herrera, Lucía Martínez-Carreras, Carlos González-Oller","doi":"10.1093/labmed/lmag045","DOIUrl":"10.1093/labmed/lmag045","url":null,"abstract":"Introduction: Hemoglobinopathies are a heterogeneous group of inherited disorders whose prevalence and phenotypic spectrum are influenced by population dynamics and diagnostic strategies. Longitudinal data from nonendemic European regions remain scarce.Methods: We conducted a retrospective longitudinal analysis of hemoglobinopathies diagnosed at a regional hospital in southern Spain over a 30-year period (1996-2025). Diagnoses were classified as thalassemia syndromes or structural hemoglobin variants and analyzed according to the native or migrant origin of the patient. Temporal trends were assessed in relation to demographic changes and evolving laboratory methods.Results: A total of 5340 hemoglobinopathies were identified: 1716 (32.1%) thalassemias and 3624 (67.9%) structural variants. Migrant patients accounted for 83.8% of diagnoses. Thalassemias predominated among native patients (88.1%), whereas structural variants, particularly hemoglobin S, predominated in migrant patients (78.6%). Annual diagnoses increased more than 5-fold during the study period, particularly after systematic high-performance liquid chromatography screening was introduced in 2008, with cases among migrant patients showing a strong linear trend (R2 = 0.86).Discussion: This 30-year experience demonstrates a major shift in the epidemiology and diagnosis of hemoglobinopathies in southern Spain. Migration and advances in laboratory diagnostics have increased the detection of structural variants and complex genotypes, highlighting the need for continuously updated diagnostic algorithms in increasingly diverse populations.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":"57 5","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13437019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148687332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resolving diagnostic complexity: long-read sequencing unmasks a Hong Kongαα/‒α4.2 genotype mimicking a heterozygous ‒α3.7 deletion on multiplex ligation-dependent probe amplification. 解决诊断复杂性：长读测序揭示了香港αα/ -α4.2基因型，模拟多重连接依赖探针扩增的杂合-α3.7缺失。

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Laboratory medicine Pub Date : 2026-08-04 DOI: 10.1093/labmed/lmag048

Youqiong Li, Bin Li, Lihong Zheng, Liang Liang

{"title":"Resolving diagnostic complexity: long-read sequencing unmasks a Hong Kongαα/‒α4.2 genotype mimicking a heterozygous ‒α3.7 deletion on multiplex ligation-dependent probe amplification.","authors":"Youqiong Li, Bin Li, Lihong Zheng, Liang Liang","doi":"10.1093/labmed/lmag048","DOIUrl":"10.1093/labmed/lmag048","url":null,"abstract":"Introduction: Complex structural variants in thalassemia are rare and often undetectable or misdiagnosed by conventional genetic testing methods. We report a case initially misclassified by multiplex ligation-dependent probe amplification (MLPA) as a heterozygous ‒α3.7 deletion that was subsequently correctly identified by third-generation sequencing (TGS) as the Hong Kongαα (HKαα)/‒α4.2 genotype.Methods: Gap polymerase chain reaction (PCR) was used to screen for 4 common deletional α-thalassemia variants (-SEA, -THAI, ‒α3.7, and ‒α4.2). A PCR-reverse dot blot detected 3 nondeletional mutations. We used MLPA to analyze copy number variations in the α-globin gene cluster; TGS characterized large deletions, cis/trans phasing, and complex structural rearrangements.Results: Gap PCR identified the female patient as a heterozygous -SEA carrier. In the male patient, gap PCR showed 3 bands (‒α3.7, wild type, and ‒α4.2), making genotyping uncertain. The MLPA findings suggested a heterozygous ‒α3.7 deletion; TGS revealed coexisting deletion and duplication fragments, leading to the final diagnosis of HKαα/‒α4.2.Discussion: For HKαα and ‒α4.2 coinheritance, gap PCR cannot provide an accurate genotype, and MLPA may erroneously report a heterozygous ‒α3.7 deletion. Third-generation sequencing accurately identified this complex variant, demonstrating its suitability for detecting such structural variants in clinical diagnostics.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":"57 5","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148709133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect size measures in laboratory medicine. 检验医学中的效应量测量。

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Laboratory medicine Pub Date : 2026-08-04 DOI: 10.1093/labmed/lmag055

Nesrin Nigussie, Michal Ordak

{"title":"Effect size measures in laboratory medicine.","authors":"Nesrin Nigussie, Michal Ordak","doi":"10.1093/labmed/lmag055","DOIUrl":"https://doi.org/10.1093/labmed/lmag055","url":null,"abstract":"Introduction: Statistical significance indicates whether an observed finding is unlikely to have occurred by chance but does not quantify the magnitude or clinical importance of that finding. Effect size measures and related quantitative measures complement P values by improving the interpretation of laboratory findings.Methods: This review examines the principal effect size measures and related quantitative measures used in contemporary laboratory medicine. It explains what each measure quantifies, when it is appropriate to use, and how it contributes to data interpretation. The application of these measures is illustrated using 40 published studies representing diverse diseases, laboratory disciplines, patient populations, and study designs.Results: Across the reviewed studies, these measures consistently provided information beyond statistical significance by quantifying differences, associations, agreement, diagnostic performance, and other analytically important characteristics of laboratory tests. They supported a more comprehensive interpretation of biomarkers, analytical methods, diagnostic tests, and clinical interventions.Discussion: Routine reporting and context-specific interpretation of appropriate effect size measures and related quantitative measures alongside P values should become standard practice in laboratory medicine to improve the interpretation, comparability, and clinical relevance of laboratory research.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":"57 5","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148889877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of sodium citrate and magnesium sulphate anticoagulation on platelet parameters measured on the Alinity hq analyzer. 柠檬酸钠和硫酸镁抗凝剂对Alinity hq分析仪测定血小板参数的影响。

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Laboratory medicine Pub Date : 2026-08-04 DOI: 10.1093/labmed/lmag069

Cristine Betzer, Rie McGrail

{"title":"Effect of sodium citrate and magnesium sulphate anticoagulation on platelet parameters measured on the Alinity hq analyzer.","authors":"Cristine Betzer, Rie McGrail","doi":"10.1093/labmed/lmag069","DOIUrl":"https://doi.org/10.1093/labmed/lmag069","url":null,"abstract":"Introduction: EDTA-dependent pseudothrombocytopenia (PTP) may cause spuriously low platelet counts and can be prevented by using alternative anticoagulants, such as sodium citrate (citrate) and magnesium sulphate (MgSO4). However, these anticoagulants may affect platelet parameters depending on the analytical platform. Their effect on platelet measurements using the Abbott Alinity hq hematology analyzer has not previously been evaluated.Methods: Blood samples from 40 outpatients without known PTP were collected in K2-EDTA, citrate, and MgSO4 tubes. Platelet count, mean platelet volume, and reticulated platelet fraction were measured on an Alinity hq analyzer. Paired comparisons were performed using Friedman repeated-measures analysis with the Dunn multiple comparisons test.Results: Compared with EDTA, median platelet counts were statistically significantly lower in citrate (-21.1%) and MgSO4 (-31.7%) than in EDTA (P < .05). Mean platelet volume was also statistically significantly reduced in citrate (-3.2%) and MgSO4 (-4.7%) (P < .05). Median reticulated platelet fraction was slightly higher in both anticoagulants, but only MgSO4 differed substantially from EDTA.Discussion: On the Alinity hq analyzer, citrate and MgSO4 result in statistically significantly lower platelet count than EDTA in samples from individuals without known PTP. Platelet parameters are therefore influenced by anticoagulant choice and should not be interpreted interchangeably.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":"57 5","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149009370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0