Laboratory medicine最新文献_第2页

Thrombin-antithrombin complex might be a promising parameter for monitoring anticoagulation in the early postoperative period after LVAD implantation. 凝血酶-抗凝血酶复合物可能是LVAD植入术后早期监测抗凝的一个有前景的参数。

IF 1

Laboratory medicine Pub Date : 2025-07-11 DOI: 10.1093/labmed/lmae113

Liqin Ling, Chaonan Liu, Jing Zhou

{"title":"Thrombin-antithrombin complex might be a promising parameter for monitoring anticoagulation in the early postoperative period after LVAD implantation.","authors":"Liqin Ling, Chaonan Liu, Jing Zhou","doi":"10.1093/labmed/lmae113","DOIUrl":"10.1093/labmed/lmae113","url":null,"abstract":"Introduction: Appropriate bridging anticoagulation is critical in the early postoperative period after left ventricular assist device (LVAD) implantation, because the patients are usually in a fragile balance of thrombotic to bleeding risk. Unfortunately, the ideal manner of monitoring postoperative bridging anticoagulation remains undetermined.Methods: Here we reported a case demonstrating that thrombin-antithrombin complex might be an option in this situation.Results: This patient suffered thrombosis and bleeding simultaneously within 7 days after implantation, and the 2 commonly used methods for monitoring bridging anticoagulation, activated partial thromboplastin time and anti-Xa activity, were incompatible with each other. After a multi-disciplinary team discussion, the clinicians decided to manage his anticoagulation based on thrombin-antithrombin complex level. It worked out well, and the patient was transferred to a general ward 2 weeks later.Discussion: With respect to clinical endpoints, thrombin-antithrombin complex might be a promising parameter for monitoring anticoagulation in the early postoperative period after LVAD implantation.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"423-427"},"PeriodicalIF":1.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple HPLC method for the determination of plasma progesterone levels in the third trimester of human pregnancy. 一种测定人妊娠晚期血浆黄体酮水平的简便高效液相色谱法。

Laboratory medicine Pub Date : 2025-07-11 DOI: 10.1093/labmed/lmae098

Mevlut Albayrak, Yucel Kadioglu, Fatma Demirkaya-Miloglu, Bunyamin Borekci

{"title":"A simple HPLC method for the determination of plasma progesterone levels in the third trimester of human pregnancy.","authors":"Mevlut Albayrak, Yucel Kadioglu, Fatma Demirkaya-Miloglu, Bunyamin Borekci","doi":"10.1093/labmed/lmae098","DOIUrl":"10.1093/labmed/lmae098","url":null,"abstract":"Progesterone is a steroid hormone primarily associated with pregnancy. A simple, rapid, and reliable high-performance liquid chromatography (HPLC) method has been developed and validated for the quantification of progesterone in human plasma. The method consists of a simple liquid-liquid extraction of progesterone and internal standard (estriol) from human plasma using a mixture of hexane and diethyl ether. The chromatographic determination of progesterone was performed using an acetonitrile-water (70:30, v/v) mobile phase with a C18 reversed-phase column. The method achieved an extraction recovery of greater than 96.4% from spiked plasma samples. Intra- and inter-day precision were generally acceptable, with relative SD% less than ≤6.60% and accuracy (relative error %) better than 3.64%. The developed and validated method was used to successfully quantify progesterone levels in plasma samples collected from women during the third trimester of pregnancy. Furthermore, a statistical comparison was conducted between progesterone concentrations in plasma samples obtained from 2 groups of pregnant women: group 1 (n = 9) at 30-35 weeks and group 2 (n = 9) at 36-41 weeks. The developed and validated HPLC method described in this study enables the successful determination of progesterone in human plasma, offering advantages such as shorter analysis time, simplicity, cost-effectiveness, and potential routine use during pregnancy.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"360-366"},"PeriodicalIF":0.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of organism recovery and staining properties for 3 methods of Gram stain preparation. 比较三种革兰氏染色制备方法的菌体回收率和染色性能。

Laboratory medicine Pub Date : 2025-07-11 DOI: 10.1093/labmed/lmae095

Audrey Pisahl, Martha Timmons, Sheila Criswell

{"title":"Comparison of organism recovery and staining properties for 3 methods of Gram stain preparation.","authors":"Audrey Pisahl, Martha Timmons, Sheila Criswell","doi":"10.1093/labmed/lmae095","DOIUrl":"10.1093/labmed/lmae095","url":null,"abstract":"Background: One possible way to improve accuracy of blood culture Gram stain analyses is increasing the concentration of organisms on the slide prepared from the blood culture broth.Methods: From each positive blood culture bottle, 1 direct smear, a 1-drop concentrated preparation, and 1 cytospin/cytofuge preparation were Gram stained and evaluated. There were 2 evaluators who ranked the 3 preparations from most to fewest organisms seen. Each preparation was also scored as acceptable or unacceptable for both organism and background stain quality.Results: The 1-drop slide exhibited the highest concentration of organisms compared with both the cytospin and direct smear but presented frequent difficulties with interpretation of Gram staining acceptability with both the background and the organisms. Although cytospin preparations are known to concentrate liquid specimens, the current study found no enrichment of microorganisms over the direct smear preparation. The cytospin was, however, advantageous for enhancing organism morphology by creating a monolayer of elements and reducing background artifacts.Conclusion: The 1-drop preparation is simple, inexpensive, and effective at increasing organism concentration for analysis, making it a good option when processing culture bottles with low organism loads, but more investigation into methods of improving stain quality is necessary.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"336-342"},"PeriodicalIF":0.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142831563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CALR frameshift mutation detection in myeloproliferative neoplasms by microfluidic chip analysis. 应用微流控芯片检测骨髓增殖性肿瘤CALR移码突变。

Laboratory medicine Pub Date : 2025-07-11 DOI: 10.1093/labmed/lmae096

Michael P Greenwood, Keith M Newton, Kristi L Pepper, Heather L Hendrickson, Randall J Olsen, Jessica S Thomas

{"title":"CALR frameshift mutation detection in myeloproliferative neoplasms by microfluidic chip analysis.","authors":"Michael P Greenwood, Keith M Newton, Kristi L Pepper, Heather L Hendrickson, Randall J Olsen, Jessica S Thomas","doi":"10.1093/labmed/lmae096","DOIUrl":"10.1093/labmed/lmae096","url":null,"abstract":"Background: CALR mutation analysis is routinely used to diagnose BCR/ABL1-negative myeloproliferative neoplasms. The 2 most common CALR mutations are a 52-base pair (bp) deletion and a 5-bp insertion, which account for approximately 85% of cases.Methods: To evaluate our new microfluidic chip assay, we tested CALR mutant and wild-type specimens that were previously analyzed using conventional methods at a reference laboratory. Samples included EDTA-anticoagulated peripheral blood and bone marrow specimens, air dried bone marrow aspirate smears, and formalin-fixed, paraffin-embedded bone marrow sections. CALR exon 9 was PCR amplified using 2 previously published primer pairs and a third unique primer pair designed for our new assay. Amplicons were sized using microfluidic chip analysis.Results: Concordance with the reference method was 100% (42/42). Intra-run and inter-run reproducibility were also 100% (3/3 and 3/3, respectively). The limit of detection was confirmed to be 6% mutant alleles.Conclusion: We determined that the microfluidic chip assay to detect CALR exon 9 mutations was acceptable for clinical use. Compared with the conventional method, the microfluidic analysis assay benefits from a streamlined workflow, faster turnaround, and a smaller instrument footprint.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"343-350"},"PeriodicalIF":0.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cracking the code of aldosterone synthase deficiency: bridging genetics and biochemistry: a case report. 破解醛固酮合成酶缺乏症的密码：连接遗传学和生物化学：一例报告。

IF 1

Laboratory medicine Pub Date : 2025-07-11 DOI: 10.1093/labmed/lmae102

Lekha Priyadharshini Kamarajan, Mala Mahto, Sushil Kumar, Pradeep Kumar

{"title":"Cracking the code of aldosterone synthase deficiency: bridging genetics and biochemistry: a case report.","authors":"Lekha Priyadharshini Kamarajan, Mala Mahto, Sushil Kumar, Pradeep Kumar","doi":"10.1093/labmed/lmae102","DOIUrl":"10.1093/labmed/lmae102","url":null,"abstract":"Objective: Aldosterone synthase deficiency (ASD) is a rare autosomal recessive inherited disease with an overall clinical phenotype of failure to thrive, vomiting, severe dehydration, hyperkalemia, and hyponatremia. Mutations in the CYP11B2 gene encoding AS are responsible for the occurrence of ASD. Defects in CYP11B2 gene have only been reported in a limited number of cases worldwide. Due to this potential life-threatening risk, a comprehensive hormonal investigation followed by genetic confirmation is essential for the clinical management of offspring.Methods: We report a case of a newborn who was found to have persistent hyponatremia, hyperkalemia, low aldosterone level, raised renin levels, normal cortisol, and normal 17 hydroxyprogesterone level, suggesting the diagnosis of isolated ASD.Results: Genetic report was suggestive of isolated ASD caused by a novel base pair deletion in exon 3, homozygous CYP11B2 variant (chr8:g.142915123_142915125del; depth: 124x d) (p.Lys175del; ENST00000323110.2). After initial steps of rehydration and salt restoration, the child was started on oral tablet fludrocortisone. The child responded well and showed a good gain in growth and development.Discussion: We elaborate on the biochemical and genetic work-up performed and describe potential pitfalls in CYP11B2 sequencing due to its homology to CYP11B1.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"419-422"},"PeriodicalIF":1.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferritin-based biomarkers and their prognostic value in Vietnamese patients with sepsis. 基于铁蛋白的生物标志物及其在越南败血症患者中的预后价值。

Laboratory medicine Pub Date : 2025-07-11 DOI: 10.1093/labmed/lmae112

Phung Thanh Huong, Huu Huy Nguyen, Thi Minh Huyen Pham

{"title":"Ferritin-based biomarkers and their prognostic value in Vietnamese patients with sepsis.","authors":"Phung Thanh Huong, Huu Huy Nguyen, Thi Minh Huyen Pham","doi":"10.1093/labmed/lmae112","DOIUrl":"10.1093/labmed/lmae112","url":null,"abstract":"Introduction: Sepsis remains a critical global health challenge because of its high mortality rates and complex pathophysiology. Early and accurate diagnosis and prognosis is pivotal for enhancing clinical outcomes in sepsis management. This study investigates the prognostic implications of serum ferritin, the ferritin index (FI), and the ferritin to lymphocyte ratio (FLR) on septic shock and 28-day mortality among Vietnamese patients with sepsis.Methods: We conducted a retrospective cohort analysis using data from medical records of 89 patients with sepsis.Results: The study establishes FI and procalcitonin cutoffs for discriminating septic shock at 2.29 and 37.15 µg/mL, respectively, with sensitivities of 71.9% and 54.4% and specificities of 56.3% and 77.4%, respectively. Combining FI and procalcitonin enhances predictive capability. Predicting 28-day mortality, serum ferritin, FLR, and Sequential Organ Failure Assessment scores have cutoffs of 828.45 µg/L, 901.41 mg/G, and 10.5, respectively, with varying sensitivities and specificities. Integration of serum ferritin value and FLR with Sequential Organ Failure Assessment score substantially improves predictive accuracy (area under the curve approaching 0.8). Subgroup analysis revealed pronounced associations, particularly serum ferritin, with acute kidney injury (odds ratio = 10.00) and anemia (odds ratio = 11.27) in predicting mortality.Discussion: This study underscores FLR's novel mortality prediction utility and reinforces ferritin biomarkers' prognostic relevance in sepsis, highlighting implications for tailored sepsis management strategies.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"413-418"},"PeriodicalIF":0.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143575019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of soluble neural cell adhesion molecule, soluble IL-2 receptor ɑ, and IL-2 in pelvic pain severity and their association with endometriosis in infertile women. 可溶性神经细胞粘附分子、可溶性IL-2受体和IL-2在不孕妇女盆腔疼痛严重程度中的作用及其与子宫内膜异位症的关系

Laboratory medicine Pub Date : 2025-07-11 DOI: 10.1093/labmed/lmae107

Fadhil Ahsan, Nanda Yuli Rahmawati, Budi Santoso, Fidyah Nanda Alditia, Alfin Firasy Mufid, Ashon Sa'adi, Sri Ratna Dwiningsih, Arif Tunjungseto, M Y Ardianta Widyanugraha

{"title":"Role of soluble neural cell adhesion molecule, soluble IL-2 receptor ɑ, and IL-2 in pelvic pain severity and their association with endometriosis in infertile women.","authors":"Fadhil Ahsan, Nanda Yuli Rahmawati, Budi Santoso, Fidyah Nanda Alditia, Alfin Firasy Mufid, Ashon Sa'adi, Sri Ratna Dwiningsih, Arif Tunjungseto, M Y Ardianta Widyanugraha","doi":"10.1093/labmed/lmae107","DOIUrl":"10.1093/labmed/lmae107","url":null,"abstract":"Introduction: Pelvic pain, often associated with endometriosis, significantly affects women's quality of life. This study explored the soluble neural cell adhesion molecules (sNCAM), soluble interleukin 2 receptor α (sIL-2Rα), and IL-2 levels in the serum and peritoneal fluid of infertile women with pelvic pain.Methods: We enrolled 86 infertile women aged 24 to 41 years undergoing diagnostic laparoscopy: 44 women with endometriosis and 42 women who acted as controls. Pain intensity was assessed using the visual analog scale. The soluble molecules were measured using enzyme-linked immunosorbent assay.Results: Serum and peritoneal sNCAM, sIL-2Rα, and IL-2 levels were statistically significantly higher in women with pelvic pain. Both serum and peritoneal sNCAM levels correlated with visual analog scale scores, indicating a relationship between these markers and pain severity. Elevated peritoneal sIL-2Rα levels were also associated with pelvic pain. Receiver operating characteristic curve analysis showed the potential of serum sNCAM in distinguishing between mild and moderate to severe pain.Discussion: Elevated levels of sNCAM, sIL-2Rα, and IL-2 in serum and peritoneal fluid correlate with pelvic pain severity in infertile women, suggesting their involvement in disease pathogenesis and potential as objective biomarkers for pain assessment in endometriosis. Further research is needed to validate these findings for clinical use.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"384-395"},"PeriodicalIF":0.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143560337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanisms of resistance, epidemiological characteristics, and molecular evolution of carbapenem-resistant hypervirulent Klebsiella pneumoniae. 耐碳青霉烯类药物的高病毒性肺炎克雷伯氏菌的耐药机制、流行病学特征和分子进化。

Laboratory medicine Pub Date : 2025-07-11 DOI: 10.1093/labmed/lmae110

Qun Wang, Mei-Yi Ye, Chi Hong, Zu-Pin Li, Lei Lin

{"title":"The mechanisms of resistance, epidemiological characteristics, and molecular evolution of carbapenem-resistant hypervirulent Klebsiella pneumoniae.","authors":"Qun Wang, Mei-Yi Ye, Chi Hong, Zu-Pin Li, Lei Lin","doi":"10.1093/labmed/lmae110","DOIUrl":"10.1093/labmed/lmae110","url":null,"abstract":"Introduction: Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) is a highly pathogenic, drug-resistant, and transmissible \"superbug\" that causes infections in hospitals and communities. Because of the lack of effective antimicrobial treatment options, morbidity and mortality from CR-hvKP infections have increased dramatically, and outbreaks and the rapid spread of CR-hvKP in hospitals have become a major global public health challenge.Methods: The mechanisms of molecular evolution in CR-hvKP include the acquisition of a hypervirulent plasmid encoding a virulence gene by carbapenemase-producing K pneumoniae, the horizontal transfer of plasmids carrying carbapenem resistance genes to hvKP, and the acquisition of fusion plasmids carrying both carbapenem resistance genes and hypervirulent genes by classic K pneumoniae. In addition, hvKP can develop a resistance phenotype under antibiotic pressure.Results: CR-hvKP arises through plasmid-mediated convergence of resistance genes and virulence factors. Its multidrug resistance and lethal pathogenicity fuel hospital outbreaks, requiring urgent action to block plasmid transmission and strengthen surveillance to contain the spread of this evolving superbug.Discussion: In this article, we have summarized the carbapenemase resistance mechanism, evolution mechanism, virulence factors, and epidemiology of CR-hvKP. Our aim was to elucidate the molecular evolutionary mechanism of CR-hvKP and provide a reference for curbing the spread of CR-hvKP.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"323-335"},"PeriodicalIF":0.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic stewardship of procalcitonin testing by implementation of computer-based decision support. 通过实施基于计算机的决策支持降钙素原测试的诊断管理。

Laboratory medicine Pub Date : 2025-07-11 DOI: 10.1093/labmed/lmae108

Gina M Belfiore, Christopher A Jankowski, Carmen L Isache, Scott Smith, Matthew Feldhammer

{"title":"Diagnostic stewardship of procalcitonin testing by implementation of computer-based decision support.","authors":"Gina M Belfiore, Christopher A Jankowski, Carmen L Isache, Scott Smith, Matthew Feldhammer","doi":"10.1093/labmed/lmae108","DOIUrl":"10.1093/labmed/lmae108","url":null,"abstract":"Introduction: Appropriate test utilization strategies are paramount in preventing the overuse of antibiotics, which can contribute to antimicrobial resistance. Biomarkers such as procalcitonin (PCT) are frequently ordered upon suspicion of infection, but current pneumonia and sepsis guidelines recommend against using PCT alone when initiating antibiotic therapy. The purpose of this study was to evaluate the effectiveness of standardized, guideline-based computerized decision support in curbing the inappropriate ordering of PCT testing.Methods: This study was a retrospective, single-center cohort of hospitalized adult patients with at least 1 available PCT serum level over a 27-month period. Secondary outcomes included the total number of PCT orders, the number of days on antibiotics, and the appropriate antibiotic response rate based on the PCT result.Results: A total of 300 patients met our inclusion criteria for this study. The rate of appropriate PCT ordering increased in the postimplementation group (2% vs 10%), with an increased rate of appropriate ordering in patients with community-acquired pneumonia (4.3% vs 18.4%) and no change in patients with sepsis (0% vs 3.3%). Overall, PCT orders dropped by 78% after implementation of decision support.Discussion: This study demonstrated that the addition of guideline-based computerized clinical decision support increased the rate of diagnostic stewardship for PCT orders.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"396-401"},"PeriodicalIF":0.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143560331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of multiple myositis-specific antibodies: prevalence and clinical significance. 多发性肌炎特异性抗体的检测：患病率及临床意义。

Laboratory medicine Pub Date : 2025-07-11 DOI: 10.1093/labmed/lmae103

Ameni Jerbi, Sahar Karoui, Mouna Snoussi, Sawsan Feki, Chifa Damak, Zouheir Bahloul, Hatem Masmoudi, Sameh Marzouk, Hend Hachcicha

{"title":"Detection of multiple myositis-specific antibodies: prevalence and clinical significance.","authors":"Ameni Jerbi, Sahar Karoui, Mouna Snoussi, Sawsan Feki, Chifa Damak, Zouheir Bahloul, Hatem Masmoudi, Sameh Marzouk, Hend Hachcicha","doi":"10.1093/labmed/lmae103","DOIUrl":"10.1093/labmed/lmae103","url":null,"abstract":"Background: The multipositivity of myositis-specific antibodies (MSAs) is uncommon. Our study aimed to assess the prevalence and the clinical relevance of multiple positive MSAs in routine practice.Methods: A 10 year single-center retrospective study (2015-2024) was conducted reviewing all samples analyzed with the Dot Myositis EUROLINE (Euroimmun Germany). Clinical data corresponding to samples with multiple positive MSAs was reviewed.Results: Among 140 samples positive for at least 1 MSA, 22 patients (15.7%) were positive for at least 2 MSAs on the same sample. The diagnosis of idiopathic inflammatory myopathies (IIMs) was confirmed in only 6 patients (27.2%). The frequency of double-positive MSAs was 100% in IIM patients and 60% in non-IIM patients. No IIM patients and 40% of non-IIM patients (P = .037) had 3 or more positive MSAs. MSA titers were higher in IIM patients than in non-IIM patients (49 vs 32 AU, P = .33). The clinical IIM subtype joined the IIM subtype corresponding to the predominant MSA. We had no mixed features of either IIM subtype or added severe prognosis.Conclusion: Detection of multiple MSAs is uncommon but possible using immunodot techniques. Taking into account MSA number, level of positivity, and clinical data helps in the interpretation of the results.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":"56 4","pages":"367-374"},"PeriodicalIF":0.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144612771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0