Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan最新文献

{"title":"Network pharmacology and experimental validation of asiaticoside protecting against diabetic kidney disease by regulating 11β-hydroxysteroid dehydrogenase type 2.","authors":"Zhu Qin, B I Peng, Zeng Jiali, Zhang Yang, H U Lidan, Cao Zhongkai, Zhu Jingyu, Jin Qinyang","doi":"10.19852/j.cnki.jtcm.2026.03.008","DOIUrl":"10.19852/j.cnki.jtcm.2026.03.008","url":null,"abstract":"<p><strong>Objective: </strong>To explore the protective effects and mechanisms of Asiaticoside (AC) against diabetic kidney disease (DKD) through network pharmacology combined with <i>in vitro</i> and <i>in vivo</i>experiments.</p><p><strong>Methods: </strong>Based on network pharmacology and RNA sequencing (RNA-Seq) analyses, combined with molecular docking, the core targets and signaling pathways of AC in the treatment of DKD were predicted and screened. Subsequently, the therapeutic effect of AC on DKD was validated in db/db mice and SV40-MES-13 cells, and its molecular mechanism was evaluated.</p><p><strong>Results: </strong>Network pharmacology and protein-protein interaction analyses identified 11-beta hydroxysteroid dehydrogenase type 2 (<i>HSD11B2</i>) as a potential key target for AC treatment of DKD. Molecular docking indicated strong affinity between AC derivative and HSD11B2. RNA-Seq analysis showed that AC significantly regulated steroid metabolism under diabetic conditions. In db/db mice, AC reduced urinary protein excretion, improved renal morphology, and protected renal function. AC also dose-dependently inhibited excessive proliferation of SV40-MES-13 cells in a high-glucose environment. Further validation showed that AC upregulated <i>Hsd11b2</i> mRNA expression and HSD11B2 protein levels.</p><p><strong>Conclusion: </strong>AC may alleviate DKD-related pathological processes by regulating <i>Hsd11b2</i> gene expression and HSD11B2 protein levels.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 3","pages":"641-651"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical characteristics of adolescents and young adults with type 2 diabetes and Traditional Chinese Medicine therapies: a single-center experience in China from 2011 to 2023.","authors":"W U Jing, Wang Le, Guo Xiangyu, Gao Kuo, Liu Jinmin","doi":"10.19852/j.cnki.jtcm.2026.03.010","DOIUrl":"10.19852/j.cnki.jtcm.2026.03.010","url":null,"abstract":"<p><strong>Objective: </strong>To clarify the clinical characteristics of type 2 diabetes in adolescents and young adults (T2DMY) patients, and their treatment strategies involving Traditional Chinese Medicine (TCM) and Western Medicine.</p><p><strong>Methods: </strong>This study was conducted at Dongfang Hospital of Beijing University of TCM. Clinical data of outpatients and inpatients from 2011 to 2023 were retrieved from the electronic medical record database with type 2 diabetes mellitus (T2DM) as the first diagnosis.</p><p><strong>Results: </strong>A total of 405 outpatients and 570 inpatients with T2DMY were included in the analysis. Compared with middle aged and elderly T2DM patients (T2DME), T2DMY patients have a higher proportion of diabetes family history and higher body mass index, poorer control of blood glucose, blood lipid, liver and kidney function, and a higher incidence of non-alcoholic fatty liver disease and gout. In terms of anti-diabetes treatment, T2DMY patients are more likely to receive metformin and insulin drugs. In terms of TCM diagnosis and treatment, compared with T2DME patients, T2DMY patients are more frequently diagnosed with the syndrome of phlegm damp trapped spleen, and more herbs such as Fuling (<i>Poria</i>) and Chenpi (<i>Pericarpium Citri Reticulatae</i>) are more frequently used to invigorate the spleen and dispel dampness.</p><p><strong>Conclusion: </strong>Compared with T2DME patients, T2DMY patients have worse metabolism. In the treatment of TCM, more attention can be paid to the function of the spleen, and the herbal medicine can be used to enhance the function of the spleen and dispell dampness when it is compatible with <i>Qi</i>tonifying drugs.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 3","pages":"706-714"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266321/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acupuncture-driven exosomes: an emerging therapy.","authors":"Jian Jiawei, Ding Zhimin, Zhuang Yueyang, Wang Junzi, Yang Minguang, Liu Weilin","doi":"10.19852/j.cnki.jtcm.2026.03.017","DOIUrl":"10.19852/j.cnki.jtcm.2026.03.017","url":null,"abstract":"<p><p>Acupuncture, as a significant component of Traditional Chinese Medicine (TCM), has attracted increased attention for its mechanism of controlling the physiological functions of the human body and promoting the recovery of diseases by stimulating specific acupoints. Despite its long history and wide clinical application, the mechanism of action of acupuncture is still not fully understood. More research needs to be done on how acupuncture affects molecular communication and cellular function. Exosomes are nanoscale vesicles that rely on cellular multivesicular bodies (MVBs) fused with cell membranes to be released into the extracellular matrix. They are crucial for information transfer between cells and are currently a hot research topic in the world's cutting-edge life sciences. Previous research has demonstrated that the therapeutic effect of acupuncture may be related to stimulating certain cells to secrete exosomes, that exosomes released may contain \"acupuncture information\", and that manipulating the back-injection of exosomes to produces \"acupuncture-like\" effects. These findings suggest that exosomes could serve as a bridge between conventional acupuncture therapy and modern precision medicine. They also offer fresh prospects and difficulties for acupuncture translational medicine research. To better define the relationship between exosomes and acupuncture, we reviewed and systematized the literature on past studies related to exosomes and acupuncture. This paper provides a significant theoretical and experimental foundation for applying exosomes in precision medicine by summarizing and analyzing the relationship between acupuncture stimulation and exosome function. This is expected to promote the combination of traditional acupuncture therapy and modern biotechnology and bring innovation and progress to future medical practice.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 3","pages":"768-778"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional Chinese Medicine for the prevention and treatment of adolescent idiopathic scoliosis: scientific understanding and a multimodal therapeutic approach.","authors":"Lin Kaifeng, Zeng Zhongliao, Sun Yan, Sun Fangzhou, Zhang Han, Sun Jing, Jiang Ling, Jeffrey D Deng, L I Min, Liang Bocheng","doi":"10.19852/j.cnki.jtcm.2026.03.015","DOIUrl":"10.19852/j.cnki.jtcm.2026.03.015","url":null,"abstract":"<p><p>Adolescent Idiopathic Scoliosis (AIS) is a common spinal disorder characterized by deviations in the coronal, sagittal, and axial planes. As AIS progresses with age, it compromises biomechanical function and increasingly impacts pulmonary function and psychological well-being. Bracing and Physiotherapeutic Scoliosis-Specific Exercises (PSSE) are commonly used conservative treatments for AIS. However, Traditional Chinese Medicine (TCM) has been gaining increasing attention in conservative AIS management. This study examines the effectiveness of TCM and its integrative therapies in AIS treatment, with particular emphasis on the foundational TCM concept of \"preventive treatment of disease\". A three-level prevention strategy is proposed, highlighting the importance of early detection, timely treatment, and proactive intervention. This framework aims to promote a scientific understanding of TCM approaches and their synergistic use with other therapies in AIS management, offering new perspectives and potential directions for future.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 3","pages":"745-756"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the anti-apoptotic, anti-platelet aggregation, and pro-angiogenic properties of Naoshuantong capsule in ischemic stroke: insights from network pharmacology and animal experiments.","authors":"L I Chuanpeng, Yao Yaoyao, X U Yingzhi, Jiang Ping, Feng Luda, Gao Ying, Dong Xinglu","doi":"10.19852/j.cnki.jtcm.20260304.001","DOIUrl":"10.19852/j.cnki.jtcm.20260304.001","url":null,"abstract":"<p><strong>Objective: </strong>To explore the complex molecular mechanisms of Naoshuantong capsule (, NST), a Traditional Chinese Medicine formula, against ischemic stroke (IS) using network pharmacology and various experimental verifications.</p><p><strong>Methods: </strong>This study employed an integrative approach that combined network pharmacology with experimental validation. Specifically, a network pharmacology model was developed to clarify the fundamental mechanisms and essential NST targets against IS. The core targets and pathways were verified using molecular docking and animal experiments. Additionally, the predicted effects of NST were confirmed by a series of experimental techniques, including hematoxylin-eosin staining, platelet aggregation, flow cytometry, and Western blotting.</p><p><strong>Results: </strong>A total of 299 targets for overlap of compounds-disease targets were predicted. Protein-protein interaction (PPI) network analysis revealed that the top 30 core targets. Subsequent molecular docking analysis demonstrated that serine-threonine protein kinase 1 (AKT1), phosphoinositide 3-kinase (PI3K), and vascular endothelial growth factor A (VEGFA) have significant binding abilities with these compounds. Gene ontology (GO) enrichment analysis indicated that NST mechanisms against IS involved anti-apoptosis, antiplatelet-aggregation, and angiogenesis ability, confirmed by animal experiments. GO pathway analysis further showed that the mechanism of NST in treating IS was associated PI3K-AKT signaling. Ultimately, this study determined that NST downregulated cleaved-caspase-3 expression while upregulated phosphorylated AKT (P-AKT)/AKT, phosphatidylinositol-4,5-Bisphosphate 3-kinase/PI3K, B-cell lymphoma-2 (Bcl-2)/Bcl-2-associated X protein, and VEGFA expressions.</p><p><strong>Conclusion: </strong>The analysis utilizing multiple approaches manifested that NST has anti-apoptotic, antiplatelet-aggregation, and pro-angiogenic properties in managing IS, offering a systemic outlook for investigating the impact of NST on IS.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 3","pages":"629-640"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the dissemination and development pathways of Traditional Chinese Medicine health knowledge in China.","authors":"Zhu Min, Guo Hua, M A Xuesheng, Zhang Xingping, Zhang Lei, Song Yujiao, Jiang Zhuonan, Y U Feilong","doi":"10.19852/j.cnki.jtcm.2026.03.014","DOIUrl":"10.19852/j.cnki.jtcm.2026.03.014","url":null,"abstract":"<p><p>Against the backdrop of China's aging society and under the macro policy of building a Healthy China proposed at the Fifth Plenary Session of the 18th Central Committee of the Communist Party of China, the dissemination of Traditional Chinese Medicine (TCM) health knowledge has emerged as particularly crucial and imperative. This paper systematically examines the current status of TCM health knowledge dissemination in China. It identifies multiple challenges in the dissemination process, including multidimensional constraints related to content dissemination, media channels, and audience characteristics. Drawing on these findings, this paper proposes a strategic framework centered on high-quality content, with standardized media platforms as key hubs and audience empowerment as the ultimate goal.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 3","pages":"740-744"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shenling Baizhu powder reduced the oxidative stress and mitochondrial damage during ulcerative colitisthe Kelch-like ECH-associated protein 1-NF-E2-related factor 2/nuclear factor kappa B pathway.","authors":"Zhang Quanhui, Zeng Jinlan, S U Liang, Wang Yi, Deng Yongwen, Zhang Xiuchai","doi":"10.19852/j.cnki.jtcm.20260304.002","DOIUrl":"10.19852/j.cnki.jtcm.20260304.002","url":null,"abstract":"<p><strong>Objective: </strong>To explore the effects and relative mechanisms of Shenling Baizhu powder (, SBP) on ulcerative colitis (UC).</p><p><strong>Methods: </strong>This study investigates the potential of SBP, a traditional Chinese herbal formula, in attenuating inflammation and oxidative stress in UC. Applying both <i>in vivo</i>(rat) and <i>in vitro</i> (human colonic epithelial cells) models, we explored the anti-inflammation and oxidative stress effect of SBP, focusing on the Kelch-like ECH-associated protein 1-NF-E2-related factor 2/nuclear factor kappa B (KEAP1-NRF2/NF-κB) pathway and mitochondrial homeostasis.</p><p><strong>Results: </strong>Results demonstrated that SBP administration significantly improved the health status of rats, with less intestinal injury, and reduced inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6) and oxidative stress (myeloperoxidase, malondialdehyde, and reactive oxygen species) both in vivo and in vitro. Moreover, SBP restored the inhibited KEAP1-NRF2/NF-κB signaling during inflammatory responses induced by dextran sulfate sodium or lipopolysaccharide, and decreased the expression level of the inflammatory molecule: NF-κB. Besides, SBP also helped to restore the mitochondrial dynamics, as evidenced by the recovery of mitochondrial membrane potential and mitofusin-2 levels.</p><p><strong>Conclusion: </strong>These findings suggest that SBP may offer a promising alternative therapy for managing UC, providing anti-inflammatory and antioxidant benefits as key molecular mechanisms.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 3","pages":"584-593"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated analysis of plasma metabolomics and proteomics reveals the biological characteristics of damp-heat and stasis-toxin syndrome in colorectal cancer.","authors":"Qian Jingyang, Zhang Qinchang, Zhang Dong, Shi Heweng, Geng Xuechen, Sun Xiaohe, Sun Dongdong, Cheng Haibo","doi":"10.19852/j.cnki.jtcm.20251204.001","DOIUrl":"10.19852/j.cnki.jtcm.20251204.001","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the biological attributes of core syndromes in colorectal cancer, namely, the damp-heat and stasis-toxin syndrome (SRYD).</p><p><strong>Methods: </strong>Between October 2021 and October 2022, a cohort comprising 40 patients with colorectal cancer (CRC) diagnosed with damp-heat and stasis-toxin syndrome (SRYD group), 40 patients with CRC without this syndrome (non-SRYD group), and 40 healthy controls (Normal group) was recruited at Jiangsu Province Hospital of Chinese Medicine. Untargeted metabolomics analysis was conducted on plasma samples from all 120 participants, while differential protein analysis using four-dimensional data-independent acquisition proteomics was performed on 20 randomly selected samples per group. A combined analysis of proteomics and metabolomics data followed, and the identified potential diagnostic biomarkers were subsequently used to train and validate multiple machine learning models.</p><p><strong>Results: </strong>Proteomic analysis revealed 130 differential proteins in the colorectal cancer with damp-heat and stasis-toxin syndrome (CRC-SRYD) group, enriched in pathways including complement and coagulation cascades, as well as nuclear factor kappa-B (NF-κB) signaling. Metabolomic analysis identified 584 differential metabolites within the same group, showing enrichment in pathways such as primary bile acid biosynthesis, central carbon metabolism in cancer, and glucagon signaling. Integrated pathway analysis indicated heightened activity of the NF-κB signaling pathway in the CRC-SRYD group. A biomarker panel, comprising 6 proteins and 9 metabolites selected through the ReliefF algorithm, was used to construct a diagnostic model with random forest, achieving an accuracy of 93.33%, sensitivity of 80.00%, and specificity of 100%.</p><p><strong>Conclusion: </strong>This study systematically elucidates plasma metabolomic and proteomic alterations in patients with CRC, establishing a robust diagnostic model for CRC syndrome (CRC-SRYD). Further investigation is warranted to clarify the underlying molecular mechanisms and biological foundations.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 3","pages":"695-705"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of electro-thumbtack needles on symptoms during high-altitude exposure: a pilot, single-blind, randomized, controlled study.","authors":"Wang Xin, Cao Xue, Qin Yuning, Sun Minglin, Yue Lihong, Tian Yukun, Wang Guangjun, Liu Xinglong, Wang Zirong, Labasangzhu, Liu Jia","doi":"10.19852/j.cnki.jtcm.2026.03.009","DOIUrl":"10.19852/j.cnki.jtcm.2026.03.009","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the efficacy and safety of electro-thumbtack needles (ETN) compared with sham acupuncture (SA) on symptoms during high-altitude exposure.</p><p><strong>Methods: </strong>In this pilot, single-blind, randomized, controlled study, we assessed the impact of ETN acupuncture on symptoms associated with high-altitude exposure. Participants were randomized into two groups: an ETN group and a SA group. The intervention involved routine skin disinfection and stimulation of seven acupuncture points: bilateral Neiguan (PC6), bilateral Hegu (LI4), bilateral Taiyang (EX-HN5), and Zhongwan (CV12), with pressure for approximately 1 min on each point. The primary outcome was the Lake Louise Score after intervention. Secondary outcome measures were heart rate, blood pressure, blood oxygen saturation, headache visual analogue scale score, clinical function score, and sleep scale scores after the intervention.</p><p><strong>Results: </strong>We enrolled 125 patients with high-altitude exposure; 59 in the ETN group and 66 in the SA group. AMS was present in 37.90% of the 125 patients. The Lake Louise Score in the ETN group was significantly lower than that in the SA group 6 h postintervention [<i>P =</i>0.05; adjusted mean difference: 0.56; 95% confidence interval (<i>CI</i>) (0.01, 1.11)]. The changes in blood pressure, blood oxygen saturation, and Groningen Sleep Quality Survey, Stanford Sleepiness Scale, visual analog scale, and clinical functioning scores 48 h postintervention showed no significant differences between the groups. Decreases in cerebral oxygen saturation postintervention differed significantly between the ETN and SA groups [<i>P</i>< 0.05; <i>t</i> = -1.39; 95% <i>CI</i> (3.50, 0.59)].</p><p><strong>Conclusions: </strong>This study assessed the feasibility of ETN to address AMS and was a preliminary evaluation of its efficacy. This study provides a basis for the development of intervention programs and scale implementation. ETN is a possible intervention in AMS for special populations, such as those with drug contraindications or pregnant women. This study may guide future research by presenting novel insights, strategic directions, and foundational evidence.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 3","pages":"674-683"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatoprotection of Jianpi Qingre Lishi prescription on non-alcoholic steatohepatitis miRNA-27/peroxisome proliferator-activated receptor gamma axis.","authors":"X U Mengjun, Yan Zixing, Chen Xi, Cai Juanjuan, Zhang Haiou, Lin Zhenwen","doi":"10.19852/j.cnki.jtcm.2026.03.004","DOIUrl":"10.19852/j.cnki.jtcm.2026.03.004","url":null,"abstract":"<p><strong>Objective: </strong>To elucidate the molecular targets and physiological mechanisms underlying the therapeutic efficacy of Jianpi Qingre Lishi prescription (, JQLP) in the treatment of non-alcoholic steatohepatitis (NASH).</p><p><strong>Methods: </strong>This study used 120 Sprague-Dawley rats to establish six groups at random (<i>n =</i> 20): blank control (BC), model, low-dose JQLP (L-JQLP), medium-dose JQLP (M-JQLP), high-dose JQLP (H-JQLP), and positive control (PC) groups. Rats in the BC group received a diet with methionine- and choline-sufficient (MCS), while those in the remaining groups were fed with methionine- and choline-deficient (MCD) diet. Blood samples were collected for biochemical analyses and inflammatory factors determination; while liver tissues were harvested to identify the histological alterations through hematoxylin-eosin staining and oil red O staining. In addition, the levels of miRNA-27 (miR-27) and peroxisome proliferator-activated receptor γ (PPARγ) were determined utilizing quantitative real-time polymerase chain reaction and Western blotting.</p><p><strong>Results: </strong>Compared to the BC group, the model group showed no significant changes in fasting blood glucose (FBG), which was substantially reduced after both M-JQLP and H-JQLP treatments. MCD diet induced a series of pathological alterations, such as extensive vacuole-like steatosis, disorganized hepatic plates, and significant inflammatory cell infiltration in liver tissues, which were dose-dependently weakened by JQLP intervention. Rats fed with MCD diet demonstrated increase in total cholesterol, triglyceride, low-density lipoprotein cholesterol, alanine aminotransferase and aspartate aminotransferase activities, but decrease in high-density lipoprotein cholesterol. JQLP treatment effectively normalized these parameters in a dose-dependent manner. Furthermore, rats with MCD diet were detected with largely secreted tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6); while JQLP intervention decreased TNF-α and IL-6 secretion, but enhanced IL-4 content. Additionally, miR-27 upregulation and PPARγ downregulation were induced in liver tissues of rats with MCD diet, which were counteracted by JQLP treatment.</p><p><strong>Conclusions: </strong>JQLP can ameliorate NASH progression, potentially through the regulation of miR-27/ PPARγ axis. JQLP may be a promising therapeutic candidate worthy of further clinical investigation for NASH treatment.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 3","pages":"561-570"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}