Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan最新文献

{"title":"Yunpiqiangshen gel improves quality of life in dialysis patients.","authors":"Y U Manshu, Jiang Chunchun, Yao Min, Hua Jianwu, Jiang Yan, Huang Min, Liu Jianjing, Zhou Yan, Wang Yuan, Sheng Meixiao","doi":"10.19852/j.cnki.jtcm.2025.01.011","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.011","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the efficacy and safety of Yunpiqiangshen gel which composed of eleven herbs on quality of life (QOL) improvement.</p><p><strong>Methods: </strong>We enrolled 180 patients lasted from January 2020 to December 2021. Seventy-eight patients received standards of care (control group) and 76 patients received Yunpiqiangshen gel [Traditional Chinese Medicine, (TCM) group] for 6 months in statistical analysis. The primary outcome assessed using Kidney Disease Quality of Life Short Form Questionnaire version 1.3 (KDQOL-SF^TM 1.3), including Short Form Health Survey (SF-36) and Kidney Disease Targeted Areas (KDTA) scores. The second outcomes included the TCM syndrome score, Fatigue Assessment Instrument (FAI) score, Modified Quantitative Subjective Global Assessment (MQSGA) score, anthropometric indicators, and blood chemical testing. Adverse events, including decompensated gastrointestinal symptoms, were evaluated.</p><p><strong>Results: </strong>The results showed that the mean change of SF-36 (75 <i>±</i> 13 <i>vs</i> 64 <i>±</i> 16, <i>F =</i> 6.070, <i>P =</i> 0.015) and KDTA (76 <i>±</i> 7 <i>vs</i> 70 <i>±</i> 9, <i>F =</i> 4.118, <i>P =</i> 0.044) scores in the TCM group had an improvement (increase) from baseline, as compared with the control group. Yunpiqiangshen gel also resulted in a significant improvement in almost dimensions of QOL. At the end of follow-up, the imputed percentage of patients in the response rate of TCM syndrome was greater in the TCM group than in the control group (76.32% <i>vs</i> 20.51%, <i>χ</i> ² = 48.02, <i>P =</i> 0.000). The fatigue, soreness of waist, anorexia, abdominal distension, loose stool, and constipation were alleviated after Yunpiqiangshen gel therapy. The FAI (98.58 <i>±</i> 25.08 <i>vs</i> 131.21 <i>±</i> 31.85, <i>F =</i> 8.745, <i>P =</i> 0.004) and MQSGA (10.13 <i>±</i> 2.84 <i>vs</i> 12.83 <i>±</i> 3.85, <i>F =</i> 11.396, <i>P =</i> 0.001) scores in the TCM group had an improvement (reduce) from baseline compared with the control group. A higher level of albumin of patients in the TCM group compared with the control group. Diarrhea, vomit, and loose stool were more common in the TCM group, but generally mild in severity.</p><p><strong>Conclusion: </strong>Compared with the standards of care, added Yunpiqiangshen gel was a safe and effective therapy for improving QOL in dialysis patients.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"123-131"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical observation of efficacy in adhesive intestinal obstruction with Integrated Chinese (Chaihu Shugan San, ) and Western Medicine: a randomized controlled trial.","authors":"Zhang Xin, Cao Jia, Luo Chengping, Fan Yibin, D U Jie","doi":"10.19852/j.cnki.jtcm.2025.01.010","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.010","url":null,"abstract":"<p><strong>Objective: </strong>To explore the treatment efficacy of integrated Chinese medicine (Chaihu Shugan San, , CSS) and western therapy in the treatment of adhesive intestinal obstruction (AIO), to provide new ideas for the management of the disease.</p><p><strong>Methods: </strong>In our single-blind randomized controlled study, 120 patients with AIO who were hospitalized in The Affiliated Hospital of China West Normal University Nan Chong Gaoping District People's Hospital from January 2021 to June 2022 and met the inclusion criteria were categorized into the treatment group and the control group. Patients from the control group were administered basic Western Medicine therapy, whereas patients from the treatment group were administered basic Western Medicine therapy plus CSS by gastric tube injection. Subsequently, the time to first anal exhaustion and defecation, time to relief of abdominal distension and pain, days of hospitalization, Traditional Chinese Medicine (TCM) symptom scores, interleukin-6 (IL-6), C-reactive protein (CRP) and procalcitonin (PCT) levels in the 2 groups were recorded and compared.</p><p><strong>Results: </strong>The comparison of clinical efficacy of the treatment group were better than the control group. The TCM symptom score was considerably lower in the treatment group; the inflammation indicators CRP, IL-6, and PCT also decreased statistically when comparing the control group. Furthermore, there were significantly reduced in the time to first exhaustion, time to first defecation, time to relief of abdominal pain and distension, and days of hospitalization in the treatment group versus the control group.</p><p><strong>Conclusion: </strong>CSS could suppress the inflammatory reaction, reduce days of hospitalization, relieve clinical symptoms in AIO patients with reliable efficacy and high safety and is worthy of clinical application.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"115-122"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture alleviates zymosan-induced colorectal hypersensitivity.","authors":"L I Siting, Wang Shaojun, Yin Yehui, D E Gejing, L I Caicai, Wang Ziyan, Cao Wenjie","doi":"10.19852/j.cnki.jtcm.20220425.001","DOIUrl":"10.19852/j.cnki.jtcm.20220425.001","url":null,"abstract":"<p><strong>Objective: </strong>this study to investigate the mechanism underlying the electroacupuncture (EA) alleviates colorectal hypersensitivity, a feature of irritable bowel syndrome (IBS).</p><p><strong>Methods: </strong>The colorectal hypersensitivity model was established by treating mice with zymosan. Electrophysiological techniques, Western blotting and immunofluorescence staining were used to detect the changes of the sensitive state of the colorectum and the response in spinal ganglion and spinal cord after acupuncture intervention.</p><p><strong>Results: </strong>colorectal distension studies revealed that repetitive applied electroacupuncture treatment on mice could significant alleviates colorectal intensity. Western blotting studies with nerve growth factor (NGF) in the colorectum, substance P (SP) in the spinal ganglion, protein kinase C gamma (PKCγ) in the spinal cord, and transient receptor potential vanilloid 1 (TRPV1) showed that electroacupuncture suppressed zymosan-induced expression of TRPV1, NGF and SP in multiple tissues. Immunofluorescence labeling results showed that EA attenuated the expression of NGF in the colorectum, SP in the spinal ganglion, PKCγ in the spinal cord, and TRPV1 in all three tissues in zymosan-treated mice. Moreover, the number of neurons double-positive for TRPV1/Isolectin B4 (IB4) and TRPV1/Neurofilament (NF) 200 was increased in the spinal ganglion.</p><p><strong>Conclusion: </strong>these results provide molecular-level evidence that EA alleviates zymosan-induced colorectal hypersensitivity by altering the expression of pain-associated proteins in the colorectum and spinal cord. EA has a potential to be therapeutic intervention option for IBS treatment.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"32-38"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of lappaconitine on Freund's complete adjuvant-induced arthritis exerted through P2X7 receptor-mediated regulation of M1/M2 balance in rats.","authors":"Zhang Pengqiang, Feng Qi, Huang Weiyan, O U Shan","doi":"10.19852/j.cnki.jtcm.2025.01.004","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.004","url":null,"abstract":"<p><strong>Objective: </strong>to investigate the anti-arthritic effects of lappaconitine (LA) on adjuvant-induced arthritis in Sprague-Dawley rats and its possible involvement in the regulation of M1/M2 macrophage balance through the P2X7 receptor (P2X7r).</p><p><strong>Methods: </strong>Rats were immunized with complete Freund's adjuvant and then intraperitoneally administered LA (2, 4, or 8 mg·kg^-1·d^-1) or methotrexate (0.5 mg/kg per 3 d) for 14 d. The anti-arthritic effects of LA were evaluated through arthritis index (AI) assessment, ankle diameter measurement, and histopathological staining analysis. The analgesic effect of LA on arthritis was measured using mechanical withdrawal threshold testing and gait scoring. The impacts of LA on macrophage polarization, the expression of pro-/anti-inflammatory cytokines and P2X7r were analyzed using quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and Western blotting.</p><p><strong>Results: </strong>LA treatment significantly reduced AI scores, paw swelling, joint destruction, and inflammatory cell infiltration, and alleviated arthritis pain. Additionally, LA promoted a balanced M1/M2 ratio by increasing the mRNA expression level of M2 marker arginase 1 and decreasing those of M1 markers inducible nitric oxide synthase and interleukin (IL)-1β in synovial tissues. Furthermore, LA lowered the levels of three M1-related cytokines, namely tumor necrosis factor-α, IL-1β and IL-18, and raised the level of the M2-related cytokine IL-10. Further research showed that treatment with LA inhibited the expression of P2X7r.</p><p><strong>Conclusion: </strong>Our findings indicate that the notable therapeutic and analgesic effects of LA on AIA rats are exerted through balancing the M1/M2 ratio, probably via P2X7r.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"39-48"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research and development of the pulse acquisition system and the pulse biomimetic reproduction system.","authors":"Y I Kai, M A Yunjing, Guo Shen, Liu Heng, X U Bin","doi":"10.19852/j.cnki.jtcm.2025.01.021","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.021","url":null,"abstract":"<p><p>The paper is an introduction to the front-end pulse acquisition and the back-end pulse biomimetic reproduction system. This system is capable of faithfully replicating the complete pulse waveform collected at the front end. Traditional Chinese Medicine (TCM) practitioners analyze and diagnose the pulse patterns at the replication end. Meanwhile, the obtained pulse waveforms are analyzed and learnt by a neural network based on key diagnostic points in TCM pulse taking, which enables the determination of the corresponding relationships between different pulse waveforms and various pulse patterns in TCM pulse taking. With the support of clinical samples, an auxiliary diagnostic system for TCM pulse patterns ensures the accuracy of pulse pattern replication.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"213-220"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of acupuncture combined with bone-setting therapy to treat tourette syndrome: a three-arm randomized controlled trial.","authors":"Lang Jiawang, Jin Lingqing, Luo Jianchang, Lang Boxu","doi":"10.19852/j.cnki.jtcm.2025.01.017","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.017","url":null,"abstract":"<p><strong>Objective: </strong>To explore the efficacy and safety of acupuncture combined with atlantoaxial joint bone-setting therapy for the treatment of Tourette syndrome.</p><p><strong>Methods: </strong>We randomly divided 600 patients at a ratio of 1∶1∶1 into three groups: group A (acupuncture combined with atlantoaxial joint bone setting therapy group), group B (acupuncture group), and group C (tiapride group). After two months of treatment, the Yale global tic severity scale (YGTSS) score reduction, improvement in social function impairment, clinical efficacy, and long-term efficacy in the three groups were compared.</p><p><strong>Results: </strong>After treatment, in the analysis of YGTSS score reduction, social function impairment improvement, the clinical control rate, and long-term efficacy, the results were all Group A > Group B > Group C, with a statistically significant difference (<i>P <</i> 0.05). However, the total clinical efficacy of treatment in group A (94.9%) was not significantly different from that in group B (91.8%). Adverse reactions did not occur in groups A and B, and several adverse reactions occurred in 29% (<i>n</i> = 58) of the group C patients.</p><p><strong>Conclusion: </strong>Compared with traditional drug therapy, acupuncture combined with atlantoaxial joint bone setting therapy has better clinical and long-term efficacy. This treatment strategy can improve the social function of children and prevent adverse reactions to drugs.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"176-183"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture enhances the mitophagy of granulosa cells in premature ovarian insufficiency model mice by inactivating the hippo-yes-associated protein/transcriptional co-activator with postsynaptic density protein, drosophila disc large tumor suppressor, and zonula occludens-1 protein binding motif pathway.","authors":"W U Jiaman, Tang Meng, Luo Yu, Zhu Haimin, Zhao Tianqi, M A Fei, Ning Yan","doi":"10.19852/j.cnki.jtcm.2025.01.002","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.002","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the potential mechanism of electroacupuncture (EA) in alleviating premature ovarian insufficiency (POI) and to provide a theoretical basis for EA treatment of POI.</p><p><strong>Methods: </strong>For this purpose, a POI mice model was developed by injecting 12 mg/kg busulfan and 120 mg/kg cyclophosphamide intraperitoneally to induce POI. It was then proceeded by EA intervention at Guanyuan (CV4) acupoint on the second day following modeling. Similarly, apoptosis in ovarian granulosa cells was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling staining, while enzyme-linked immunosorbent assay was employed for measuring serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E₂), and anti-müllerian hormone (AMH) levels. Moreover, transmission electron microscopy (TEM) was employed for examining mitochondrial morphology, while autophagy and hippo-yes-associated protein/transcriptional co-activator with postsynaptic density protein, drosophila disc large tumor suppressor, and zonula occludens-1 protein binding motif (YAP/TAZ) pathway related protein levels in ovarian tissue were detected <i>via</i> Western blotting.</p><p><strong>Results: </strong>Analysis of serum levels of various hormones indicated that serum FSH and LH were reduced in EA compared to the POI group, while E₂ and AMH levels were found to be elevated in EA compared to the POI group. The EA was found to inhibit apoptosis in granulosa cells in POI model mice, in addition to improved mito-chondrial damage and significantly improved mitophagy. Pathway analysis revealed that EA was involved in activating the hippo-YAP/TAZ pathway, followed by reversing EA effects on granulosa cell apoptosis and mitophagy with the use of verteporfin, an autophagy and YAP-T-cell factor/enhancer of split and activator of transcription domain family member interaction inhibitor.</p><p><strong>Conclusions: </strong>EA at the Guanyuan (CV4) acupoint protected the granulosa cell by inhibiting cell apoptosis and promoting mitophagy, which was mediated by the Hippo-YAP/TAZ pathway.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"13-21"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Difference of the gut microbiota of premature ovarian insufficiency in two traditional Chinese syndromes.","authors":"W U Jiaman, Ning Yan, Tan Liya, M A Fei, Lin Yanting, Zhuo Yuanyuan","doi":"10.19852/j.cnki.jtcm.2025.01.012","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.012","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the differences in gut microbial characteristics between two traditional Chinese syndromes of premature ovarian insufficiency (POI).</p><p><strong>Methods: </strong>Forty women with POI were recruited from the Department of Traditional Chinese Medicine at Shenzhen Maternity and Child Healthcare Hospital between June and December 2020. Women with POI were divided into the kidney deficiency and blood stasis syndrome (SDBS) and <i>Qi</i> and blood deficiency syndrome (QBDS) groups. Gut microbial community profiles were analyzed by 16S rRNA gene sequencing using an Illumina MiSeq system. A retrospective study comparing hormone levels and gut microbiota information was performed between the SDBS and QBDS groups.</p><p><strong>Results: </strong>Compared with the QBDS group, the serum levels of estradiol (E2) and anti-Müllerian hormone (AMH) were significantly decreased in the SDBS group. The quantities of Adlercreutzia, Eggerthella, Klebsiella, and Paraprevotella significantly increased in the SDBS group, whereas Lactobacillus decreased significantly. Moreover, alterations in the microbiome in the SDBS and QBDS groups were closely related to the levels of E2 and AMH. The area under the receiver operating characteristic curve for the classification of the two syndromes by the gut microbiome was 0.71.</p><p><strong>Conclusions: </strong>There were significant differences in the dominant microbiota between the SDBS and QBDS groups, and the change in Proteobacteria in the QBDS group was more significant. The characteristics of gut microbiota help us differentiate between the SDBS and QBDS groups, which may provide a basis for the objectification of TCM syndrome types.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"132-139"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect and mechanism of Sanqi () in treating periodontitis.","authors":"A N Yuanyuan, Liu Wang, L I Yanjie, Wang Yanchun, Ren Xiaobin, H E Hongbing","doi":"10.19852/j.cnki.jtcm.20241111.001","DOIUrl":"10.19852/j.cnki.jtcm.20241111.001","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect and mechanism of Sanqi (<i>Radix Notoginseng</i>) in treating periodontitis.</p><p><strong>Methods: </strong>The active components and periodontitis targets were analyzed through network pharmacology and molecular docking. A rat model of periodontitis was established and rats were treated by continuous intragastric administration of Sanqi (<i>Radix Notoginseng</i>) at different doses for 30 d. The alveolar bone structure was observed by micro-CT, the periodontal tissue structure was observed by hematoxylin-eosin staining, and the related proteins changes was detected by immunohistochemical staining.</p><p><strong>Results: </strong>Sanqi (<i>Radix Notoginseng</i>) and periodontitis had a total of 96 coincident targets that were significantly enriched in the interleukin 17 (IL-17), tumor necrosis factor (TNF), and advanced glycation endproducts and the receptor of advanced glycation endproducts signaling pathways. The active compound quercetin had good binding activity with interleukin 6 (IL-6), vascular endothelial growth factor A (VEGFA), matrix metallopeptidase 9 (MMP9), tumor necrosis factor α (TNF-α), Jun proto-oncogene (JUN), and C-X-C motif chemokine ligand 8 (CXCL8) in periodontitis. Compared with normal group, the distance from the cementoenamel junction (CEJ) to the alveolar bone (AB) was increased, alveolar bone absorption was obvious, the periodontal tissue structure was disorganized, and IL-6 and TNF-α were upregulated in periodontitis group; meanwhile, the distance from CEJ to AB was significantly decreased, alveolar bone resorption was reduced, periodontal tissue structure was improved, the expression of IL-6, TNF-α, IL-17 and retinoid-ralated orphan receptor γt (RORγt) were decreased, Forkhead Box P3 (FOXP3) and IL-10 were increased after Sanqi (<i>Radix Notoginseng</i>) treatment.</p><p><strong>Conclusions: </strong>Sanqi (<i>Radix Notoginseng</i>) improves the structure of alveolar bone and gum, and reduces inflammation; the mechanism involve in inhibiting IL-17 signaling pathway to suppress Th17 and promote Treg cells differentiation.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"66-75"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacology and animal experiments revealed the protective effects of Guilong prescription on chronic prostatitis and its possible mechanisms.","authors":"Zhu Peixuan, S U Zeqi, Fan Qiongyin, Zhang Cai, Wang Ting","doi":"10.19852/j.cnki.jtcm.20240423.002","DOIUrl":"10.19852/j.cnki.jtcm.20240423.002","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the protective effects of Guilong prescription (, GL) on chronic prostatitis (CP) and unravel the underlying mechanisms of its pharmacological effects.</p><p><strong>Methods: </strong>The composition of GL was determined <i>via</i> linear ion trap/electrostatic field orbital trap tandem high-resolution mass spectrometry, and the identified compounds were performed network pharmacological analysis to predict possible pathways of the effects of GL on CP. A CP rat model was established by carrageenan, and rats were randomly assigned into a Control group, Sham group, CP group, GL low dose (3.5 g/kg) group, GL medium dose (7 g/kg) group, and GL high dose (14 g/kg) group. Hematoxylin-eosin staining of the prostate, and prostate blood -perfusion measured by laser speckle contrast analysis were used to evaluate the efficacy of GL. Expression of intercellular cell adhesion molecule-1 (ICAM-1) and induce nitric oxide synthase (iNOS) were determined by immunohistochemistry, and the content of interferon-γ (IFN-γ), interleukin-1β (IL-1β), interleukin-4 (IL-4), interleukin-10 (IL-10), chemokine ligand 1 (CXCL1) and tumor necrosis factor-α (TNF-α) were determined by electro-chemiluminescence assays. The expression of p38 mitogen-activated protein kinase (p38 MAPK), phosphatidylinositol 3-kinase (PI3K), ribosome-associated complex-alpha serine/threonine-protein kinase (Akt), nuclear factor-κ-gene binding p65 (NF-κB p65), inhibitor of NF-κB-α (IκBα), glycogen synthase kinase-3β (GSK-3β), and their phosphorylated forms were tested by Western blot.</p><p><strong>Results: </strong>In GL, a total of 48 compounds were identified, including 14 flavonoids, 14 alkaloids, 11 carboxylic acids, 4 lactones, 2 glycosides, 2 terpenoids and 1 aldehyde. Network pharmacological analysis suggested that the mechanism of GL may be related to PI3K-Akt signaling pathway and cytokine expression. After treatment with GL, inflammatory pathological changes in the prostate of rats were significantly improved, and blood perfusion of the prostate was significantly decreased. GL reduced the expression of IFN-γ, CXCL1, TNF-α, IL-1β, iNOS, ICAM-1, p38 MAPK, p-p38 MAPK, PI3K, p-PI3K, NF-κB, p-NF-κB, IκBα, p-IκBα, GSK-3β, p-GSK-3β, p-Akt in CP rats, and increased the expression of IL-4 and IL-10 in CP rats.</p><p><strong>Conclusion: </strong>The chemical compositions of GL were first identified. GL can improve pathological changes in the prostate and recover the prostate blood perfusion of CP rats. The possible mechanisms of GL on CP involve increasing the expression of anti-inflammatory cytokines IL-4 and IL-10, inhibiting pro-inflammatory cytokines TNF-α, IL-1β, and IFN-γ, and down regulating the expression of CXCL1, iNOS, and ICAM-1 <i>via</i> inhibiting PI3K-Akt and NF-κB signaling pathway.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"89-99"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}