Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan最新文献

{"title":"Effect of electroacupuncture on hypertensive and sympathetic excitability mechanism mediated by the paraventricular nucleus of the hypothalamus in spontaneous hypertensive rats.","authors":"Sun Jiao, Wang Yueming, Lyu Jian, Liu Xin, Yue Bingnan, L I Yinyin, Liu Jipeng, Sun Yize, Liu Qingguo, Yan Liu","doi":"10.19852/j.cnki.jtcm.2025.03.013","DOIUrl":"10.19852/j.cnki.jtcm.2025.03.013","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the mechanism of electroacupuncture of sympathetic nerve activity and blood pressure reduction in the hypothalamic paraventricular nucleus (PVN) of spontaneous hypertensive rats (SHRs).</p><p><strong>Methods: </strong>A total of 64 male SHRs were divided into four groups: model, sham-operated (Sham), electro-acupuncture (EA), and N-methyl-D-aspartate receptor antagonist and electroacupuncture (NRA + EA). In addition, 16 Wistar-Kyoto rats were used as controls. PVN stereotaxic surgery was performed in both the Sham and NRA + EA groups, while the EA and NRA + EA groups received 14 d of electroacupuncture. Blood pressure (BP) and heart rate (HR) were measured the day before the intervention and every other day. After 14 d of intervention, the rats in each group were tested for renal sympathetic nerve activity (RSNA). The associated factor levels were determined using Western blotting, reverse transcription-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA) and immunofluorescence assays.</p><p><strong>Results: </strong>In comparison to the model group, the EA and NRA + EA groups had significantly lower BP, HR, and RSNA (<i>P <</i> 0.01). The expression of N-methyl-D-aspartate receptor (NMDAR), angiotensin II (Ang II), angiotensin II type 1 (AT1), tumor necrosis factor-α, interleukin-1β, norepinephrine and arginine vasopressin was significantly lower in the EA and NRA + EA groups (<i>P <</i> 0.01). Moreover, the antihypertensive effect of NRA+EA group outperformed to the EA group.</p><p><strong>Conclusions: </strong>Electroacupuncture effectively reduced the BP and sympathetic nerve excitability in SHRs. The mechanism was linked to the inhibition of NMDAR-mediated Ang II /AT1 and the inflammatory response in PVN.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 3","pages":"586-596"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12134317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hamayou () protein hydrolysate ameliorates depression by regulating the mitogen-activated protein kinase pathway.","authors":"L I Weijia, L U Jing, M A Chao, Liu Mengmeng, Pei Ke, Chen Hongyan, Lin Zhe, Lyu Guangfu","doi":"10.19852/j.cnki.jtcm.2025.03.007","DOIUrl":"10.19852/j.cnki.jtcm.2025.03.007","url":null,"abstract":"<p><strong>Objective: </strong>To clarify the effect of Hamayou (<i>Oviductus Ranae</i>) protein hydrolysate (ORPH) on depression and its exact underlying mechanism from a new perspective.</p><p><strong>Methods: </strong>We used the Chronic Unpredictable Mild Stress (CUMS) method to prepare a mouse model of depression and lipopolysaccharide (LPS) to prepare a model of BV2 cellular inflammation to investigate the antidepressant effect and mechanism of action of ORPH. Behavioral changes in mice and cerebral blood flow were detected by behavioral experiments and scatter imaging. Levels of corticosterone (CORT), proinflammatory cytokines and neurotransmitter were detected by enzyme-linked immunosorbent assay. Furthermore, hematoxylin-eosin staining, Tunel staining were used to evaluate the effect of ORPH. The distribution and expression of ionized calcium bindingadaptor molecule-1 (Iba-1) in mouse hippocampal tissue and BV2 cells were detected by immunofluorescence. Mitogen-activated protein kinase (MAPK) pathway related protein expression was detected by Western blot.</p><p><strong>Results: </strong>ORPH improved depression-like behavior, ameliorated brain tissue damage and apoptosis, and inhibited microglia activation in brain tissue in mice. In addition, ORPH reduced expression of B-cell lymphoma-2 (Bcl-2)-associated X (Bax), cysteinyl aspartate specific proteinase 3 (Caspase3), cysteinyl aspartate specific proteinase 9 (Caspase9), nuclear factor- kappa B (NF-κB), phosphorylation-p38 (p-p38), phosphorylation-Jun N-terminal kinase (p-JNK) proteins, and increased expression of Bcl-2, inhibitory kappa B alpha (IκB-α), phosphorylation-extracellular regulated protein kinases 1/2 (p-ERK1/2) proteins. On the other hand, there were fewer Iba-1-positive cells, lower expression of NF-κB, p-p38, p-JNK and p-ERK1/2 proteins, and higher expression of IκB-α proteins in BV2 cells in the ORPH group. In addition, ORPH increased 5-hydroxytryptamine, norepinephrine levels and decreased CORT, interleukin-1β (IL-1β), interleukin -6 (IL-6), tumor necrosis factor-α (TNF-α) levels.</p><p><strong>Conclusion: </strong>ORPH was able to improve depression-like behaviors and that it took effects by promoting cerebral blood flow, inhibition of hypothalamic-pituitary-adrenal axis overactivation, improving the structural damage of hippocampal tissues, and inhibiting the inflammatory response. ORPH can reduced neuronal damage and inhibiting apoptosis by promoting the MAPK pathway.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 3","pages":"493-507"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12134328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of Dan Ze mixture against lipotoxic cardiomyopathy through activating B-cell lymphoma-2 adenovirus E1B 19 kDa-interacting protein 3/mitophagy signaling pathway.","authors":"Shi Cheng, Chen Jian, Zhang Yufang, Gao Ya, L I Dantong, Yue Shijun, Zhang Yixin","doi":"10.19852/j.cnki.jtcm.2025.03.010","DOIUrl":"10.19852/j.cnki.jtcm.2025.03.010","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate the mechanism of Dan Ze mixture (, DZM) in the treatment of lipotoxic cardiomyopathy.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Ultra-performance liquid chromatography tandem mass spectrometry was employed to characterize the serum migration constituents of DZM. A lipotoxic cardiomyopathy rat model was established through high-fat diet and intervened by different doses of DZM. The cardiac function was assessed using echocardiography, and hematoxylin and eosin, oil red O, and Masson staining were conducted to evaluate morphological changes, lipid accumulation, and fibrosis in myocardial tissue. Serum myocardial enzyme activity, lipid levels, and lipid content of myocardial tissue were measured, while fluorescent staining and colorimetry were used to assess oxidation levels in myocardial tissue. Mitochondrial membrane potential was detected by 5,5', 6,6'-Tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanineio-dide (JC-1). Transmission electron microscopy was employed to observe ultrastructure and mitochondrial structure changes in myocardial tissue. Fluorescence double staining and colocalization were utilized to observe the binding of autophagosomes and mitochondria, while immunohistochemical staining was used to detect the expression of mitophagy-related proteins. Terminal deoxynucleoitidyl transferase mediated nick end labeling staining was employed for the identification of apoptosis in myocardial tissue, while quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) and Western blot were utilized for the detection of apoptosis, B-cell lymphoma-2 adenovirus E1B 19 kDa-interacting protein 3 (BNIP3)/ mitophagy signaling pathway-related genes and proteins. In palmitic acid-induced Rat H9C2 cardiomyocytes (H9c2) cells, various cellular parameters including cell viability, lactate dehydrogenase release, apoptosis rate, oxidative stress level, mitochondrial structure and function, and mitophagy level were assessed after the treatment of DZM drug-containing serum for a duration of 24 h. The cellular expressions of BNIP3/mitophagy signaling pathway relevant genes and proteins were further evaluated using qRT-PCR and Western blot techniques.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 295 prototypes (e.g., phenolic acids, quinones, terpenoids) were identified in serum of rats after oral administration of DZM. &lt;i&gt;In vivo&lt;/i&gt;, DZM therapy has been shown to effectively enhance cardiac function, mitigate high-fat diet-induced myocardial structural damage and lipid accumulation. Furthermore, DZM has demonstrated the ability to reduce lipid levels, attenuate cell apoptosis, combat oxidative stress, enhance mitochondrial structure and function, and activate the BNIP3/mitophagy signaling pathway. Furthermore, the silencing of BNIP3 has been shown to exacerbate palmitic acid-induced damages in H9c2 cells, while inhibiting the BNIP3/mitophagy signaling pathway can mitigate the inhibitory effects of DZM on pa","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 3","pages":"538-551"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12134320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical observation on edema based on the thermal sensitivity measurement method.","authors":"Tian Zheng, D U Jing, Liao Wenhao, L U Jixiang, Masayuki Yagi, Yoshikazu Yonei","doi":"10.19852/j.cnki.jtcm.2025.03.017","DOIUrl":"10.19852/j.cnki.jtcm.2025.03.017","url":null,"abstract":"<p><strong>Objective: </strong>To observe and analysis of the influence of the 12 meridians on edema, the constant value of the detection of the thermal sensitivity was further verified, which provided a basis for the diagnosis and identification of edema by the thermal sensitivity measurement method.</p><p><strong>Methods: </strong>A total of 1426 patients who attended Doshisha University in Japan and Xi'an Chengxintang Clinic from March 2019 to March 2023 were selected for observation and analysis and divided into 3 groups according to their diseases; 112 patients with edema in Group 1, 488 symptomatic patients with non-edema in Group 2, and 826 asymptomatic subjects in Group 3. The Thermal Sensitivity Measurement was conducted in the twelve meridians and compared among the groups. Multivariate analysis was performed to estimate meridians useful for diagnosing edema.</p><p><strong>Results: </strong>In the healthy physiological state, the value of thermal sensitivity is relatively constant, which may be affected by age factors. Through the stepwise analysis, left Taiyin lung, right Jueyin pericardium, left Shaoyang Sanjiao, bilateral Jueyin liver, right Shaoyang gallbladder, right Taiyang bladder, and right Shaoyin kidney were extracted as effective meridians for diagnosing edema. Its accuracy was high, with receiver operating characteristic, area under the curve (ROC AUC) of 96%. These findings were not incompatible with the edema-associated meridians in the Traditional Chinese Medicine.</p><p><strong>Conclusion: </strong>The thermal sensitivity measurement is thought to be useful in diagnosing edema and predicting prognosis by combining appropriate meridians. In the future, we will conduct more detailed research on how this method is useful for evaluating medical care and acupuncture treatments.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 3","pages":"639-650"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12134318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional Chinese Medicine five-tone intelligent diagnosis and treatment system.","authors":"Zhang Jingzhi, Zhang Xingzhao, Liu Yao, Zhou Yuanzi, Wang Site, Zhou Hanyu, Zhu Hongguang, Lin Facai, Xing Zhenlong","doi":"10.19852/j.cnki.jtcm.2025.03.021","DOIUrl":"10.19852/j.cnki.jtcm.2025.03.021","url":null,"abstract":"<p><p>Under the background of Artificial Intelligence (AI) and medical data informationization, intelligent Traditional Chinese Medicine (TCM) emerges as a new medical model. In this study, we introduce Affective Computing into the diagnosis and treatment process of TCM Five-Tone Therapy. And proposes an intelligent diagnosis and treatment system including Five-tone diagnosis, monitoring and treatment, to achieve intelligent music matching. This system based on sound-image database for standardized collection and diagnosis of the Five-tone. The assessment and monitoring process are then visually presented, and an emotion analysis model is established by using affective computing. On the other hand, the system employs virtual reality (VR) visual media music and somatosensory music to play and intelligently match the Five-tone, solving issues in the clinical treatment of Five-tone therapy, such as the difficulties of quantifying emotional data, limited ability for generalization feature extraction, and relatively insufficient research on the intelligence of Five-tone therapy. This study can promote the integration of technology in the fields of healthcare, information, and music, thereby enhancing the accuracy of diagnosis and treatment. It holds significant importance in advancing the intelligent development of Traditional Chinese Medicine diagnosis and treatment.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 3","pages":"702-710"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12134327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying potential biomarkers in the hippocampus of chronic fatigue syndrome rats treated with moxibustion at Zusanli (ST36): a proteomics study.","authors":"Feng Chuwen, L I Chaoran, Yang Yan, Q U Yuanyuan, Sun Zhongren, Sun Weibo, Liu Tingting, L I Shulin, Yang Tiansong","doi":"10.19852/j.cnki.jtcm.2025.03.012","DOIUrl":"10.19852/j.cnki.jtcm.2025.03.012","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To observe the effects of moxibustion at Zusanli (ST36) on rats with chronic fatigue syndrome (CFS) and to analyze the mechanisms of moxibustion through hippocampal Proteomics.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Male Sprague-Dawley (SD) rats were randomly divided into three groups: control group (CON), model group (MOD), and moxibustion group (MOX), with 12 rats in each group. The MOD and MOX groups underwent chronic multi-factor stress stimulation for 35 d to establish the CFS model. After modeling, the rats in the MOX group received mild moxibustion at Zusanli (ST36) (bilateral) for 10 minutes daily for 28 d. During the treatment period, rats in both the MOD and MOX groups continued modeling, while the CON group was kept under normal breeding conditions. The general condition of the rats was monitored, and behaviors were assessed using the Open Field Test (OFT), Exhaustion Treadmill Test, and Morris Water Maze (MWM). Hematoxylin and eosin (HE) staining and transmission electron microscopy (TEM) were employed to observe morphological changes in the hippocampus. Label-free Proteomics were utilized to identify differentially expressed proteins (DEPs) in the hippocampus, followed by bioinformatics analysis. The reliability of the Proteomics results was verified using Parallel Reaction Monitoring.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A: Moxibustion at Zusanli (ST36) significantly reduced the general condition score of CFS rats, improved their behavioral performance in OFT, treadmill and MWM, and repaired the pathological and synaptic structural damage in the hippocampus.B: We identified DEPs by applying a fold change threshold of 1.2 and a significance level of &lt;i&gt;P&lt;/i&gt; &lt; 0.05. In the comparison between the CON and the MOD, we identified a total of 72 DEPs (31 up-regulated and 41 down-regulated) associated with the development of CFS. In the comparison between the MOX and the MOD group, we identified a total of 103 DEPs (40 up-regulated and 63 down-regulated) related to the therapeutic effects of moxibustion. Gene Ontology (GO) enrichment analysis showed that CFS and moxibustion treatment were related to multiple biological processes, molecular functions, and cellular components. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that CFS pathogenesis was linked to base excision repair, steroid biosynthesis, and systemic lupus erythematosus, Furthermore, the treatment of CFS with moxibustion was relevant to terpenoid skeleton biosynthesis.C: Compared with the two comparison groups, we identified 16 potential biomarkers, noting that moxibustion reversed the up-regulation of 14 DEPs and the down-regulation of 2 DEPs in CFS. These proteins are mainly associated with synaptic plasticity, ribosomal function, neurotransmitter secretion, glycine metabolism, and mitochondrial function.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Moxibustion at Zusanli (ST36) is effective in treating CFS, the potential biomarker","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 3","pages":"571-585"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12138249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological effect and possible mechanism of Mudan Huaban recipe on melasma in mice induced by ultraviolet B and progesterone.","authors":"Liu Xiaoyao, L I Jialin, Wang Weiling, Fan Qiongyin, S U Zeqi, H E Cheng, Wang Chunguo, Gao Jian, Wang Ting","doi":"10.19852/j.cnki.jtcm.2025.03.001","DOIUrl":"10.19852/j.cnki.jtcm.2025.03.001","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the chemical components of Mudan Huaban recipe (, MHR) and evaluate its efficacy and possible mechanism in melasma mice.</p><p><strong>Methods: </strong>The chemical compositions of MHR were determined by the ultra-high performance liquid chromatography coupled with quadrupole-exactive mass spectrometry method. Female C57BL/6 mice were exposed to ultraviolet B and progesterone for 21 d to induce melasma, and Fontana-Masson staining was used to assess the effects of MHR on melasma. Luteinizing hormone (LH), estradiol, and follicle-stimulating hormone levels were detected by enzyme-linked immunosorbent assay. The superoxide dismutase (SOD) activity and malonic dialdehyde content were detected by chemiluminescence. Tyrosinase and related proteins expressions were detected by Western blots and immunohistochemistry.</p><p><strong>Results: </strong>A total of 43 chemical components were identified in MHR, MHR significantly decreased the melanin particles of melasma mice. MHR treatment significantly reversed the high contents of LH and low activity of SOD in models. MHR significantly reduced the higher levels of tyrosinase, tyrosinase-related proteins-1 (TRP-1), TRP-2, microphthalmia-associated transcription factor and phosphorylation of cyclic adenosine monophosphate response element-binding protein (p-CREB)/CREB in the skin of melasma mice.</p><p><strong>Conclusions: </strong>MHR protects against melasma <i>via</i> regulation of sex hormones, oxidative stress, and melanogenesis-related proteins, suggesting its possible use as a supplement and alternative drug therapy for melasma.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 3","pages":"518-527"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12134326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Weifuchun ( ) exerts therapeutic effects on gastric fundic gland polyps by promoting ferroptosis.","authors":"L I Yue, Deng Jinyan, P I Shanshan, Zhang Yingjuan, Zhao Dan, Guo Yi, Y E Yong'an, Zao Xiaobin, D U Hongbo","doi":"10.19852/j.cnki.jtcm.20250515.001","DOIUrl":"10.19852/j.cnki.jtcm.20250515.001","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the therapeutic effects of Chinese medicine Weifuchun (WFC, ) on gastric fundic gland polyps (FGPs).</p><p><strong>Methods: </strong>FGPs organoids were constructed with patients-derived samples. The morphology and size of FGPs organoids were detected using bright-field imaging. Effective components and corresponding potential targets of WFC were screened using multiple open-source databases and research on Traditional Chinese Medicine or compound formulas. Core genes were identified through protein-protein interaction networks. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses of the core genes were conducted. The interactions between main components and core targets were analyzed through the FerrDb database. The expressions of core targets were detected by quantitative real-time polymerase chain reaction (qRT-PCR).</p><p><strong>Results: </strong>After WFC treatment, the number and size of FGPs organoids were significantly reduced. Twenty nine active drug components and 162 candidate targets of WFC for treating FGPs were identified, including 37 targets related to ferroptosis. Quercetin, Glaucocalyxin B, Melissoidesin U, Melissoidesin O, Hesperetin, Glaucocalyxin A, Angustifolin, Melissoidesin M, Di-n-octyl phthalate, and beta-sitosterol were identified as the main active compounds. SRC proto-oncogene, non-receptor tyrosine kinase, signal transducer and activator of transcription 3, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta, phosphoinositide-3-kinase regulatory subunit 1, and AKT serine/threonine kinase 1 were identified as the primary targets. KEGG pathways related to carcinogenesis, cell proliferation and metabolism, and oxidative stress. WFC promoted FGPs organoids' death and could be reversed by ferroptosis inhibitor of Erastin. The qRT-PCR results showed that WFC treatment could regulate the mRNA expression levels of solute carrier family 7 member 11, acyl-CoA synthetase long chain family member 4, and arachidonate 15-lipoxygenase, type B.</p><p><strong>Conclusion: </strong>WFC may exert its therapeutic effects by inducing ferroptosis in FGPs cells.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 3","pages":"618-627"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12134325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acupuncture with different frequencies for tobacco dependence: a randomized controlled trial.","authors":"Chen Shumin, Liu Zhenyu, J I Jia, Chang Jin, L I Zhihua, Wang Yingying, Yang Jinsheng","doi":"10.19852/j.cnki.jtcm.2025.03.022","DOIUrl":"10.19852/j.cnki.jtcm.2025.03.022","url":null,"abstract":"<p><strong>Objective: </strong>To observe the effect of different acupuncture frequencies on the abstinence rate which could be used as a reference for optimizing acupuncture cessation programs.</p><p><strong>Methods: </strong>From July 2018 to June 2022, a total of 220 smokers were recruited based on inclusion criteria and randomly divided into the high-frequency acupuncture group (HF group, <i>n =</i> 110): 5 times a week from the 1st to the 4th week, from weeks 5 to 8, three times a week and the low-frequency acupuncture group (LF group, <i>n</i> = 110): 3 times a week from the 1st to the 4th week, from weeks 5 to 8, twice a week, then treated for 8 weeks and follow-up at 1 month in Beijing.</p><p><strong>Results: </strong>In total, 162 subjects completed the whole study with a drop-out rate of 20.45%. The expiratory CO point abstinence rate was HF group 53/110 (48.18%) <i>vs</i> LF group 41/110 (37.27%) in Week 8 (<i>P =</i> 0.102) and HF group 46/110 (41.82%) <i>vs</i> LF group 45/110 (40.91%) in Week 12 (<i>P =</i> 0.891) and the HF acupuncture and LF acupuncture were nearly equal in the 8-week abstinence rate. In addition, both HF and LF acupuncture significantly reduced Fagerstrőm test for nicotine dependence scale (FTND) scores (<i>P <</i> 0.05), Minnesota nicotine withdrawal scale (MNWS) scores (<i>P <</i> 0.05), and Brief Questionnaire of Smoking Urges scale (QSU-Brief) scores (<i>P <</i> 0.05), but HF acupuncture showed some superiority over LF acupuncture in relieving patients' smoking cravings (<i>P</i> < 0.05).</p><p><strong>Conclusions: </strong>The study initially showed that both high-frequency acupuncture and low-frequency acupuncture treatment were safe and effective on smoking cessation for 8 weeks, but high-frequency acupuncture was advantageous in reducing smoking cravings. More accurate acupuncture frequency needs to be validated through larger clinical studies to optimize acupuncture smoking cessation programs.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 3","pages":"651-659"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12134313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bushen Tongluo recipe improves oxidative stress homeostasis, inhibits transforming growth factor/Notch signaling pathway, and regulates the lncRNA maternally expressed gene 3/miR-145 axis to delay diabetic kidney disease.","authors":"X U Bojun, Tao Tian, Zhao Liangbin, Zheng Hui, Zhan Huakui, Guo Julan","doi":"10.19852/j.cnki.jtcm.2025.03.011","DOIUrl":"10.19852/j.cnki.jtcm.2025.03.011","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effect of Bushen Tongluo recipe (BSTLR, ) on rats with diabetic kidney disease (DKD) and to explore the underlying mechanism of action.</p><p><strong>Methods: </strong>The rat model of DKD was established, and rats were treated with different doses of BSTLR. Body weight and the levels of urinary protein, α1-microglobulin, glucose, blood urea nitrogen, creatinine, Cystatin C, superoxide dismutase, malondialdehyde, and catalase were analyzed biochemically or by enzyme-linked immunosorbent assay. The pathological damage to renal tissues was assessed by hematoxylin-eosin staining. Immunohistochemical staining was carried out to detect the expression levels of fibronectin, E-cadherin, α-smooth muscle actin, laminin, vimentin, collagen type Ⅳ in kidney tissues. Western blot analysis was conducted to analyze the expression levels of Nephrin, Desmin, Podocin, transforming growth factor-β1, mothers against decapentaplegic homolog 3 (Smad3), Notch1, jagged, hairy and enhancer of split 1 (Hes1) in kidney tissues, and the expression levels of maternally expressed gene 3 (MEG3) and miR-145 were measured by quantitative reverse transcription-polymerase chain reaction. Moreover, dual-luciferase reporter assay was employed to verify the binding of miR-145 to MEG3.</p><p><strong>Results: </strong>BSTLR increased the body weight of DKD rats, effectively ameliorated the renal function and pathological injury in DKD, regulated the balance of renal oxidative stress, inhibited the TGF/Notch signaling pathway, and affected the variations in the lncRNA MEG3/miR-145 axis.</p><p><strong>Conclusion: </strong>BSTLR improved oxidative stress homeostasis, inhibited the TGF/Notch signaling pathway, and regulated the lncRNA MEG3/miR-145 axis, effectively delaying the progression of DKD.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 3","pages":"561-570"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12134311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}