Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan最新文献

{"title":"Electroacupuncture improves cyclophosphamide-induced bladder overactivity by reducing mechanotransduction in the rat urothelium.","authors":"H U Junwei, Feng Jiwei, L I Wen, Liu Lumin, L I Xu, X U Ge, Liu Jiandang, Chen Yuelai","doi":"10.19852/j.cnki.jtcm.2025.02.003","DOIUrl":"10.19852/j.cnki.jtcm.2025.02.003","url":null,"abstract":"<p><strong>Objective: </strong>To investigate whether electroacupuncture (EA) therapy for overactive bladder (OAB) exerts its effect by modulating mechanosensitive channels in the urothelium, thereby improving bladder sensory function.</p><p><strong>Methods: </strong>In this study, a rat model of OAB was created by using intraperitoneal injections of cyclophosphamide. We performed either EA or bladder perfusion with HC-067047 [a transient receptor potential vanilloid 4 (TRPV4) antagonist] and assessed the efficacy of electro-acupuncture in the treatment of OAB in rats <i>via</i>urodynamic determination and Void spot assay. tissue morphology, distribution and expression of the TRPV4 protein and the amount of adenosine triphosphate (ATP) and Ca2+ released from urothelial cells in each group of rats were observed to identify the mechanism by which electroacupuncture improves OAB in rats.</p><p><strong>Results: </strong>EA ameliorated bladder function and voiding behaviour, improved bladder uroepithelial tissue morphology, and significantly reduced the immun-ofluorescence intensity and the mRNA and protein expression levels of TRPV4 in the uroepithelium of OAB rats. Moreover, the simulated mechanical stimulation-induced increases in Ca²⁺ concentration and the release of ATP and acetylcholine (Ach) from bladder urothelial cells were inhibited. The changes in EA followed the same trend as those in HC-067047.</p><p><strong>Conclusions: </strong>These results suggest that EA inhibits bladder sensory function by downregulating the expression of mechanically activated TRPV4 ion channels distributed in bladder urothelial cells, which correspondingly decreases the inward flow of extracellular Ca²⁺ and reduces the release of ATP and Ach, thereby attenuating excitatory signals.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 2","pages":"348-358"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture stimulation of auricular concha region improves loss of control over stress induced depression-like behavior by modulating 5-hydroxytryptamine 1A receptor.","authors":"L I Yongfeng, Chen Xinyi, Ren Wei, Qiao Haifa","doi":"10.19852/j.cnki.jtcm.2025.02.014","DOIUrl":"10.19852/j.cnki.jtcm.2025.02.014","url":null,"abstract":"<p><strong>Objective: </strong>To observe whether electroacupuncture stimulation of auricular concha region (EA-ACR) on behavior changes of depression by loss of control over stress model (LOC), and whether its effect is improved by regulating the expression levels of hydroxytryptamine (serotonin, 5-HT) 1A receptor (5-HT_1AR)/ hydroxytryptamine (serotonin, 5-HT) 2A receptor (5-HT_2AR) in hippocampus.</p><p><strong>Methods: </strong>LOC was prepared using a Skinner box, and EA-ACR to observe behavioral changes, and Western Blot was used to detect the changes of 5-HT_1AR/5-HT_2AR in the hippocampus, and then observe the changes of EA-ACR behavior after microinjection of 5-HT_1AR/5-HT_2AR antagonist into the hippocampus.</p><p><strong>Results: </strong>EA-ACR improve depressive-like behavior, up-regulated 5-HT_1AR expression and down-regulated 5-HT_2AR expression in hippocampal brain area. EA-ACR did not improve depression-like behavior after hippocampal microinjection of 5-HT_1AR antagonist, while injection of 5-HT_2AR antagonists can improve depression-like behaviors.</p><p><strong>Conclusion: </strong>EA-ACR can improve depressive-like behaviors. Loss of control over stress leads to up-regulation of 5-HT_1AR and down-regulation of 5-HT_2AR in the hippocampus, while EA-ACR mainly improves depressive behavior by regulating 5-HT_1AR in Hip.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 2","pages":"326-334"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fuzheng Xuanfei Huashi prescription suppresses inflammation in lipopolysaccharide-induced lung injury in mice toll-like recptor 4/nuclear transcription factor κB and cyclooxygenase-2/prostaglandin E2 pathway.","authors":"Huang Haiyang, Zhu Shumin, Zhong Shaowen, Liu Ying, Hou Shaozhen, Gao Jie, O U Jianzhao, Dong Mingguo, Ning Weimin","doi":"10.19852/j.cnki.jtcm.2025.02.018","DOIUrl":"10.19852/j.cnki.jtcm.2025.02.018","url":null,"abstract":"<p><strong>Objective: </strong>To determine the effect of Traditional Chinese Medicine (TCM) Fuzheng Xuanfei Huashi prescription (, FZXF) on lipopolysaccharide (LPS)-induced pneumonia in mice and identify the mechanism of FZXF in the treatment of LPS-induced lung inflammation.</p><p><strong>Methods: </strong>The pneumonia model was established by intraperitoneal injection of 5 mg/kg LPS in mice. Cytokines were detected by enzyme-linked immune-osorbent assay (ELISA), macrophages in lung tissue were determined by immunofluorescence, and pathway-related data were determined by quantitative real-time polymerase chain reaction (qPCR) and Western blot.</p><p><strong>Results: </strong>The liver, thymus, and spleen index values and the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) obviously increased in LPS-treated mice. FZXF decreased the white blood cell count and reduced the increase in the lung wet weight/dry weight ratio caused by LPS. The hematoxylin-eosin staining result showed that FZXF could maintain the integrity of lung tissue structure, alleviate interstitial oedema and alveolar wall thickening, and reduce inflammatory cell infiltration. Moreover, FZXF markedly reduced the expression of proinflammatory cytokines. FZXF also significantly reduced LPS-induced malondialdehyde production and increased superoxide dismutase level in the lung. By immunofluorescence, we found that FZXF could reduce macrophage infiltration. The mRNA expression levels of cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2), toll-like receptor 4 (TLR4) and nuclear transcription factor κB (NF-κB) in the lung tissue of mice were decreased by treatment with FZXF. In addition, FZXF inhibited the protein expression of TLR4, p-p65 and COX-2. These results indicated that FZXF could inhibit the inflammatory response of LPS induced cytokine storm in mice through TLR4/NF-κB and COX-2/PGE2 signaling pathway.</p><p><strong>Conclusion: </strong>These findings were suggested that FZXF prescription suppresses inflammation in LPS-induced pneumonia in mice <i>via</i> TLR4/NF-κB and COX-2/ PGE2 pathway.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 2","pages":"272-280"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xiahuo Pingwei San attenuated intestinal inflammation in dextran sulfate sodium-induced ulcerative colitis mice through inhibiting the receptor for advanced glycation end-products signaling pathway.","authors":"Huang Jiaen, Luo Qing, Dong Gengting, Peng Weiwen, H E Jianhong, Dai Weibo","doi":"10.19852/j.cnki.jtcm.2025.02.006","DOIUrl":"10.19852/j.cnki.jtcm.2025.02.006","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the therapeutic effects of Xiahuo Pingwei San (, XHPWS) on ulcerative colitis (UC) in mice and to explore the underlying mechanisms through a network pharmacology approach.</p><p><strong>Methods: </strong>Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was utilized to identify the chemical composition and authenticate the active constituents of XHPWS, ensuring rigorous quality control across batches. A dextran sulfate sodium (DSS)-induced UC model was established in C57BL/6 mice, which were treated with XHPWS <i>in vivo</i>. The efficacy against UC was assessed by measuring parameters such as body weight, disease activity index (DAI) scores, and colon length. Levels of inflammatory cytokines, including interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor-alpha (TNF-α), in colonic tissue were evaluated using enzyme-linked immunosorbent assay (ELISA). Histological analysis of colon sections was conducted using hematoxylin and eosin staining. A network pharmacology approach was employed to explore the mechanisms of XHPWS and to predict its potential targets in UC treatment. Predicted protein expressions in colonic tissue were validated using immune-ohistochemistry (IHC) and Western blotting techniques.</p><p><strong>Results: </strong>XHPWS effectively alle <i>via</i>ted DSS-induced UC symptoms in mice, as evidenced by restored body weight, reduced colon shortening, and decreased DAI scores. Histopathological examination revealed that XHPWS significantly reduced intestinal inflammatory infiltration, restored intestinal epithelial permeability, and increased goblet cell count. Network pharmacology analysis identified 63 active compounds in XHPWS and suggested that it might target 35 potential proteins associated with UC treatment. Functional enrichment analysis indicated that the protective mechanism of XHPWS could be related to the advanced glycation end products-receptor for advanced glycation end products (AGE-RAGE) signaling pathway. Notably, quercetin, kaempferol, wogonin, and nobiletin, the main components of XHPWS, showed strong correlations with the core targets. Additionally, experimental validation demonstrated that XHPWS significantly decreased levels of inflammatory cytokines interleukin 6 (IL-6), interleukin 1 beta (IL-1β), and tumor necrosis factor alpha (TNF-α) in UC mice, while downregulating the expression of proteins related to the AGE-RAGE pathway.</p><p><strong>Conclusion: </strong>Our study demonstrated that XHPWS effectively alle <i>via</i>tes colitis symptoms and inflammation in UC mice, potentially through the regulation of the AGE-RAGE pathway. These findings provide strong evidence for the therapeutic potential of XHPWS in UC treatment, thereby broadening its clinical applications.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 2","pages":"311-325"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of acupuncture on serum levels of hypothalamic-pituitary-adrenal axis related hormones and immune factors in rats with allergic rhinitis.","authors":"Kang Jiale, D U Zhongming, Guo Wei, D U Shuo, Han Guanxiong, Chen Sheng","doi":"10.19852/j.cnki.jtcm.2025.02.020","DOIUrl":"10.19852/j.cnki.jtcm.2025.02.020","url":null,"abstract":"<p><strong>Objective: </strong>To explore the correlation between acupuncture treatment for allergic rhinitis (AR) and hypothalamic-pituitary-adrenal (HPA) axis regulation by investigating changes in serum immune factors, HPA axis-associated hormone levels, activation levels of paraventricular nucleus (PVN) neurons, and the severity of nasal mucosal lesions, in rats with AR before and after acupuncture treatment.</p><p><strong>Methods: </strong>After establishing the AR rat model, ovalbumin was administered <i>via</i>nasal drip to all groups except the blank control. Each group received continuous treatment for 14 d: the acupuncture, acupuncture + RU-486 (mifepristone), and RU-486 groups received acupuncture only, RU486 intraperitoneal injection and acupuncture, and RU-486 intraperitoneal injection only, respectively. Following the intervention period, behavioral scoring was performed on all AR rats, and peripheral blood, nasal mucosa samples, and brains tissue (containing PVN region) were obtained following euthanization. Interleukin (IL-4, IL-5, IL-13), interferon gamma (IFN-γ), corticosterone (CORT), and corticotrophin-releasing hormone (CRH) levels were evaluated in peripheral blood samples. Adrenocorticotropic hormone (ACTH) levels were determined using an enzyme-linked immunoassay assay, and hematoxylin and eosin staining was performed on the nasal mucosa samples. The expression levels of c-Fos in PVN neurons following acupuncture treatment were evaluated by immunofluorescent staining.</p><p><strong>Results: </strong>Following the intervention period, the behavioral scores for the blank control group were lower than those of other groups (<i>P <</i> 0.05), while the acupuncture group scores were lower than those in the model control, acupuncture + RU486, and RU486 groups (<i>P <</i> 0.05). The blank control group had lower serum IL-4, IL-5, and IL-13 levels than those in the other groups (<i>P <</i> 0.05). The acupuncture group had lower serum IL-4, IL-5, and IL-13 levels than those in the model control, acupuncture + RU486, and RU486 groups (<i>P <</i> 0.05). The blank control group had the highest serum IFN-γ levels among all groups, followed by the acupuncture group. The serum CORT, CRH, and ACTH levels in the blank control group were lower than those in the remaining groups (<i>P <</i> 0.05). These biomarker levels were also lower in the acupuncture group than those in the model control, acupuncture + RU486, and RU486 groups (<i>P <</i> 0.05). Compared with model, rats in the acupuncture group exhibited an increased c-Fos expression in PVN neurons.</p><p><strong>Conclusion: </strong>Acupuncture can alleviate AR symptoms and regulate serum inflammatory factor levels and HPA axis-related hormones in AR rats. Moreover, these effects are inhibited by glucocorticoid antagonists, suggesting that acupuncture may regulate AR symptoms through HPA axis regulation.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 2","pages":"359-367"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Huoxue Chubi decoction on protein kinase B-mammalian target of rapamycin autophagy pathway in scleroderma Balb/c model mice.","authors":"Chen Xi, Q U Tiange, Jia Hui, Duan Xingwu, L I Jianhong, Zhang Kaihui, Zhang Runtian, Wang Ruijie","doi":"10.19852/j.cnki.jtcm.2025.02.005","DOIUrl":"10.19852/j.cnki.jtcm.2025.02.005","url":null,"abstract":"<p><strong>Objective: </strong>To explore the mechanisms by which Huoxue Chubi decoction (, HXCB) affects the protein kinase B (Akt)-mammalian target of rapamycin (mTOR) autophagy pathway in scleroderma Balb/c model mice.</p><p><strong>Methods: </strong>A scleroderma model was established in male Balb/c mice, followed by daily administration of HXCB (4.6, 2.3 and 1.15 g·kg^-1·d^-1) for 4 weeks. Bodyweight, epidermal and dermal thickness, dermal collagen levels, cutaneous reactive oxygen species (ROS) levels, Akt, Phosphorylated Akt (p-Akt), mTOR, Phosphorylated mTOR (p-mTOR), B-celllymphoma-2-interacting myosin-like coiled-coil protein 1 (Beclin-1) and microtubule-associated protein A/B-light chain 3 (LC3) protein and messenger ribonucleic acid (mRNA) expression were assessed.</p><p><strong>Results: </strong>HXCB treatment significantly reduced epidermal and dermal thickness, dermal collagen levels, ROS levels and the mRNA and protein expression of factors in the Akt-mTOR signaling pathway compared to the scleroderma model group. Conversely, mice body weight and autophagy factors Beclin-1 and LC3 were significantly increased in mice receiving HXCB treatment. Moreover, finally, ROS expression positively correlated with skin thickness, collagen contents and the mRNA expression levels of Akt, while the protein and mRNA expression levels of Akt-mTOR pathway-related factors were inversely correlated with the protein and mRNA expression of Beclin-1 and LC3.</p><p><strong>Conclusion: </strong>HXCB can regulate autophagy by invigorating <i>Qi</i>and promoting blood circulation, thereby reducing blood stasis, facilitating new tissue generation, and contributing to scleroderma treatment. This effect may be attributed to the promotion of autophagy and enhancement of collagen degradation through the reduction of tissue oxidative stress elicited by HXCB.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 2","pages":"303-310"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating network pharmacology and experimental validation to uncover the synergistic effects of Huangqi ()-Ezhu () with 5-fluorouracil in colorectal cancer models.","authors":"Tan Xiying, G U Ruxin, Tao Jing, Zhang Yu, Sun RuiQian, Yin Gang, Zhang Shuo, Tang Decai","doi":"10.19852/j.cnki.jtcm.2025.02.004","DOIUrl":"10.19852/j.cnki.jtcm.2025.02.004","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To evaluate the effects of Huangqi (&lt;i&gt;Radix Astragali Mongolici&lt;/i&gt;)-Ezhu (&lt;i&gt;Rhizoma Curcumae Phaeocaulis&lt;/i&gt;) (HQEZ) on colorectal cancer therapies and to elucidate the potential mechanisms of HQEZ, especially in combination with 5-Fluorouracil (5-FU).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The anti-tumor effects of HQEZ were evaluated in colorectal cancer models both &lt;i&gt;in vivo&lt;/i&gt; and &lt;i&gt;in vitro&lt;/i&gt;. The network pharmacological assay was used to investigate potential mechanisms of HQEZ. Potential target genes were selected by Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, protein-protein interaction network (PPI) and molecular docking. Within key targets, potential targets related to drug sensitivity, especially the sensitivity to 5-FU, were evaluated in HCT116 &lt;i&gt;in vitro&lt;/i&gt; by immunofluorescence, quantitative real-time polymerase chain reaction (qPCR) and Western-blot. Then, changes in potential targets were assessed in tumors from tumor-bearing mice and the expression of these targets was also evaluated in colorectal cancer (COAD) patients from the Cancer Genome Atlas Program (TCGA) database.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;HQEZ significantly enhanced the anti-tumor activity of 5-FU &lt;i&gt;in vivo&lt;/i&gt; and inhibit the growth of HCT116 &lt;i&gt;in vitro&lt;/i&gt;. By network pharmacological analysis, key targets, such as protein kinase B (AKT1), epidermal growth factor receptor (EGFR), adenosine triphosphate (ATP) binding cassette subfamily B member 1 (ABCB1, also named multidrug resistance protein 1, MDR1), ATP binding cassette subfamily G member 2 (ABCG2), thymidylate synthetase (TYMS, also named TS), prostaglandin-endoperoxide synthase 2 (PTGS2), matrix metallopeptidase 2 (MMP2), MMP9, toll like receptor 4 (TLR4), TLR9 and dihydropyrimidine dehydrogenase (DPYD), were identified. Additionally, 4 potential core active ingredients (Folate, Curcumin, quercetin and kaempferol) were identified to be important for the treatment of colorectal cancer with HQEZ. In key targets, chemoresistance related targets were validated to be affected by HQEZ. Furthermore, 5-FU sensitivity related targets, including MDR1, TS, EGFR, ribonucleotide reductase catalytic subunit M1, Breast and Ovarian Cancer Susceptibility Protein 1 (BRCA1) and mutl homolog 1 were also significantly reduced by HQEZ both &lt;i&gt;in vitro&lt;/i&gt; and &lt;i&gt;in vivo&lt;/i&gt;. Finally, these validated key targets and 5-FU sensitivity related targets were demonstrated to be up-regulated in COAD patients based on TCGA database.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;HQEZ has synergistic effects on the anti-tumor activity of 5-FU in the treatment of colorectal cancer both &lt;i&gt;in vivo&lt;/i&gt; and &lt;i&gt;in vitro&lt;/i&gt;. The beneficial effect of HQEZ results from the inhibition of the drug sensitivity targets associated with 5-FU. The combination therapy of HQEZ with 5-FU or other chemotherapeutic drugs will also improve the anti-tumor efficacy of chemot","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 2","pages":"385-398"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Huluan decotion on cyclophosphamide-induced autoimmune premature ovarian failure in murine models.","authors":"Feng Guiling, Zhou Xiaolin, Shen Chengwan, L I Panxiao, Abulizi Abudula","doi":"10.19852/j.cnki.jtcm.20240927.001","DOIUrl":"10.19852/j.cnki.jtcm.20240927.001","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the therapeutic effect of Huluan decotion (, HLD) on cyclophosphamide-induced premature ovarian failure (POF) in mice and its regulatory mechanisms.</p><p><strong>Methods: </strong>Female BALB/c mice were administered cyclophosphamide and administered received different doses of HLD for 28 d. Levels of sex hormone, such as estradiol (E2), follicle stimulating hormone (FSH) and luteinizing hormone (LH) in the sera, were assessed using enzyme-linked immunosorbent assay (ELISA). Follicular structure variances were observed through hematoxylin and eosin (HE) staining, while Forkhead box L2 (FOXL2) expression were analyzed <i>via</i> immune-ohistochemical staining. The primary mechanism of POF were investigated through Western blot analysis.</p><p><strong>Results: </strong>E2 levels decreased, and FSH and LH levels increased in POF model mice, but these trends were reversed with HLD or premarin administration, the expressions of WNT family member 4 (Wnt4), β-Catenin and FOXL2 were downregulated in POF model mice, whereas high expression levels were observed in control mice and other groups.</p><p><strong>Conclusion: </strong>HLD effectively treats POF induced with cyclophosphamide in mice by enhancing expressions of Wnt4, β-Catenin and FOXL2.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 2","pages":"266-271"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacology approach to unveiling the mechanism of berberine in the amelioration of morphine tolerance.","authors":"Han Shuai, Du Zhikang, Wang Zirui, Huang Tianfeng, G E Yali, Shi Jianwen, Gao Ju","doi":"10.19852/j.cnki.jtcm.2025.02.012","DOIUrl":"10.19852/j.cnki.jtcm.2025.02.012","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the mechanism underlying the effect of the Huanglian decoction (, HLD) on morphine tolerance (MT), using network pharmacology, and to verify these mechanisms <i>in vitro</i> and <i>in vivo</i>.</p><p><strong>Methods: </strong>Available biological data on each drug in the HLD were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform. The target proteins of MT were retrieved from the GeneCards, PharmGkb, Therapeutic Target Database, DrugBank, and Online Mendelian Inheritance in Man databases. Information regarding MT and the drug targets was compared to obtain overlapping elements. This information was imported into the Search Tool for the Retrieval of Interacting Genes/Proteins platform to obtain a protein-protein interaction network diagram. Then, a \"component-target\" network diagram was constructed using screened drug components and target information, <i>via</i>Cytoscape (Institute for Systems Biology, Seattle, WA, USA). The database for annotation, visualization, and integrated discovery was used for Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathways analyses. Pathway information predicted by network pharmacology was verified using animal studies and cell experiments.</p><p><strong>Results: </strong>Network pharmacology analysis identified 22 active compounds of HLD and revealed that HLD partially ameliorated MT by modulating inflammatory, apoptosis, and nuclear factor kappa B (NF-κB) signaling pathways. Berberine (BBR), one of the main components of HLD, inhibited the development of MT in mice. BBR reduced cell viability while increasing B-cell lymphoma 2 (Bcl-2) protein expression and decreasing CD86, NF-κB, Bax, and Caspase-3 protein expression in brain vascular 2 (BV2) mcroglia cells treated with morphine. Additionally, BBR contributed to a reduction in pro-inflammatory cytokine release and apoptotic cell number.</p><p><strong>Conclusions: </strong>BBR, a key component of HLD, effectively suppressed microglial activation and neuro-inflammation by regulating the NF-κB and apoptosis signaling pathways, thereby delaying MT. This study offers a novel approach to enhance the clinical analgesic efficacy of morphine.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 2","pages":"376-384"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypolipidemic effect and mechanism of Hedan tablet based on network pharmacology.","authors":"H U Huiming, Weng Jiajun, Tang Fangrui, Wang Yaqi, Fan Shengxian, Wang Xuecheng, Cui Can, Shao Feng, Zhu Yanchen","doi":"10.19852/j.cnki.jtcm.2025.02.015","DOIUrl":"10.19852/j.cnki.jtcm.2025.02.015","url":null,"abstract":"<p><strong>Objective: </strong>To examine Hedan tablet (HDT, )'s potential mechanisms in hyperlipidemic rats induced by a high-fat diet (HFD), as well as its regulatory effects and primary active constituents.</p><p><strong>Methods: </strong>By using ultra-performance liquid chromatography (UPLC)-quadrupole-time-of-flight (QTOF)-tandem mass spectrometry (MS/MS), the components of HDT that can enter the circulatory system were found, aiming to investigate its active constituents with pharmacological effects. Based on network pharmacology approaches, the relevant HDT targets in the therapy of hyperlipidemia were anticipated. The possible mechanism of HDT for hyperlipidemia treatment was verified by <i>in-vivo</i> experiments, and the main active components of HDT for hyperlipidemia treatment were analyzed <i>via in-vitro</i> experiments.</p><p><strong>Results: </strong>UPLC-QTOF-MS/MS identified 30 components of HDT entering the circulatory system, primarily consisting of flavonoids, diterpenoids and alkaloids. The results of a network pharmacology study revealed that 30 active components mostly target 74 genes associated with hyperlipidemia. The primary active ingredients may include quercetin, kaempferol, and epicatechin, and the main gene targets may be tumor necrosis factor (TNF), interleukin-6 (IL-6), interleukin 1 beta (IL-1β), etc. The results of animal experiments demonstrated that HDT can significantly regulate the blood lipid level in rats with HFD, improve the degree of inflammatory infiltration in rat liver cells, lower TNF-α, C-reactive protein (CRP), IL-6, matrix metalloproteinase 9 (MMP9) and malondialdehyde (MDA) levels while raising total superoxide dismutase (T-SOD) level. Meanwhile, HDT can considerably lower the expression of sterol regulatory element-binding transcription factor 2 (SREBF2), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), and MMP9 while significantly increasing the expression of peroxisome proliferator-activated receptor alpha (PPAR-α) and PPAR-γ. <i>In vitro</i> study confirmed that quercetin and kaempferol could reduce the levels of IL-6, IL1B, MMP9 and HMGCR in the high-fat model of hepatoma G2 cells.</p><p><strong>Conclusions: </strong>The mechanism by which HDT treats hyperlipidemia involves modification of the lipid metabolism targets such as downregulating SREBF2, HMGCR and MMP9, and upregulating PPAR-α and PPAR-γ, as well as anti-inflammatory and antioxidant actions. This study provides a pharmacological and biological rationale for the use of HDT in clinical hyperlipidemia management.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 2","pages":"408-421"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}