Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan最新文献

{"title":"Systematic understanding of mechanism of Shenfu decoction improve the prognosis of ischemic stroke using a network pharmacology and animal experiment approach.","authors":"Zhang Wei, Ren Changhong, Gao Chen, X U Jun, W U Xiaodan, Yang Yong","doi":"10.19852/j.cnki.jtcm.2025.05.014","DOIUrl":"10.19852/j.cnki.jtcm.2025.05.014","url":null,"abstract":"<p><strong>Objective: </strong>To explore the active compounds and the mechanism of Shenfu decoction (, SFD) against ischemic stroke (IS) through network pharmacology and animal experiments.</p><p><strong>Methods: </strong>SFD components were retrieved from the Traditional Chinese Medicine (TCM) database. The Online Mendelian Inheritance in Man (OMIM), Comparative Toxicogenomics Database (CTD) and Therapeutic Target Database (TTD) database were used to retrieve the IS-related disease targets. The herb-compound-target network was built by Cytoscape 3.7.1 software. The core targets were obtained using protein-protein interaction (PPI) network. The core targets of SFD were further analyzed through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). We then performed molecular docking between the hub proteins and key active compounds. Finally, we conducted animal experiments to verify the regulation of SFD on apoptosis following IS.</p><p><strong>Results: </strong>There were 221 corresponding targets and 25 components related to Chinese medicine throughout the compound-target network. The core targets of SFD in the treatment of IS was tumor protein P53 (Tp53), mitogen-activated protein kinase 3 (MAPK3), MAPK1, heat shock proteins 90AA1 and alpha serine/threonine-protein kinase1. There were 221 GO items in GO function enrichment analysis and 106 signaling pathways in KEGG, mainly including negative regulation of the apoptosis process, vascular endothelial growth factor signaling pathways, NOD-like receptor signaling pathway, etc. Among them, Tp53, MAPK3, and MAPK1 were docked with small molecule compounds. Through animal research, we confirmed the effect of SFD on apoptosis following stroke.</p><p><strong>Conclusion: </strong>This study demonstrates that SFD can treat IS through multiple targets and pathways, and provides new perspectives for exploring the core targets and mechanisms of SFD against IS.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 5","pages":"1078-1086"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12454265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Chang'an decoction on ulcerative colitis by regulating T helper 17 cells and regulatory T cellsRab27 in the p53/high mobility group box 1 pathway.","authors":"Zheng Li, Jin Ting, Wang Xiaojing, Wang Yingqi, Liu Fengbin, M I Hong","doi":"10.19852/j.cnki.jtcm.2025.05.007","DOIUrl":"10.19852/j.cnki.jtcm.2025.05.007","url":null,"abstract":"<p><strong>Objective: </strong>To explore the effect of Chang'an decoction (, CAD) of ameliorating the immune imbalances in ulcerative colitis (UC) by regulating Rab27 in the P53/high mobility group box 1 pathway.</p><p><strong>Methods: </strong>The functions and important signaling pathways of the Rab27- and UC-related genes were analyzed <i>via</i>the use of microarray data from the gene expression omnibus database, gene ontology database, Kyoto encyclopedia of genes and genomes database and gene set enrichment analysis. Dextran sulfate sodium salt-induced colitis mouse model was used to verify the bioinformatics results. Colon length, body weight, and disease activity index were measured. Hematoxylin and eosin staining was applied to validate the histopathology. Tight junction proteins were detected by immunohistochemistry. The proportions of T helper 17 cells (Th17) and regulatory T cells (Treg) in mesenteric lymph nodes were measured <i>via</i>flow cytometry. Proinflammatory cytokines like interleukin (IL) 17 (IL-17), IL-21 and IL-22 and anti-inflammatory cytokines like transforming growth factor β and IL-10 in the serum and colon of mice were detected by enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction, respectively. The expression levels of high mobility group box 1 (HMGB1), P53 and phospho- P53 (P-P53) in colonic tissues were detected by immunofluorescence and Western blotting.</p><p><strong>Results: </strong>Bioinformatics analysis revealed that compared with normal tissues, the expression of Rab27 was significantly increased in UC tissues. Receiver operating characteristic curve showed that Rab27 has the potential to be used as a biomarker for the diagnosis of disease activity. Enrichment analysis showed that UC and Rab27 were mainly associated with small molecule transport, nutrient metabolism, transmembrane transport and the downstream pathway of P53. According to animal experiments, the expression of Rab27 was increased in UC tissues, which aggravated the colonic pathological damage, activated the expression of HMGB1, and also leaded to the imbalance of Th17 and Treg cells. After CAD intervention, Rab27 overexpression, weight loss, colon shortening, and pathological damage were substantial reduced, the expression of tight junction proteins, zona occludens 1 and Occludin were increased. The effect of CAD at high-dose was more obvious. In addition, CAD upgraded the number of Treg cells and the production of TGF-β and IL-10, while decreasing the number of Th17 cells and the expression of inflammatory cytokines (IL-17, IL-21, and IL-22). Moreover, colon inflammation was alleviated by CAD, as indicated by the regulation of HMGB1 and P-P53 expression.</p><p><strong>Conclusion: </strong>The expression of Rab27, HMGB1 and P-P53 could be decreased by CAD, and the balance of Th17 and Treg cells as well as their related cytokines could be regulated by CAD.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 5","pages":"998-1008"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12453985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of Yangxiao Fukang granule in the treatment of stage Ⅲ hepatitis B related liver cancer: study protocol for a randomized controlled trial.","authors":"L I Shanyi, Gao Qi, L I Yanjie, Zhang Yaling, Cehn Xiaoqi","doi":"10.19852/j.cnki.jtcm.2025.05.018","DOIUrl":"10.19852/j.cnki.jtcm.2025.05.018","url":null,"abstract":"<p><p>Primary liver cancer (PLC) is the third major cause of cancer related death, which seriously affects the survival period and quality of life of patients. However, there is currently no evidence to prove which treatment method is most effective. Traditional Chinese Medicine (TCM) has shown certain advantages in the treatment of PLC, especially in the side effects of Western Medicine. Therefore, we designed a clinical trial protocol for the treatment of PLC using TCM granules. Our purpose is to explore the efficacy and safety of Yangxiao Fukang granule (YXFKG, ) in the treatment of stage III hepatitis B related PLC. A total of 216 patients from three hospitals in Henan Province will be enrolled and randomly divided into a trial group and a control group in a 1∶1 ratio. The trial group will be treated with conventional western medicine plus YXFKG, while the control group will receive conventional western medicine plus a placebo for YXFKG. All patients will receive a daily dose of either YXFKG or a placebo for six months, followed by a six-month follow-up period. The main observation outcome includes 1-year survival rate, while secondary outcomes include conversion rate to remission, objective response rate, progression free survival, overall survival, quality of life score, and TCM clinical symptom score. Blood routine, urine routine, stool routine, electrocardiogram, liver and kidney function, coagulation function test, and D-dimer are safety indicators. Collect data before treatment and during the 3rd, 6th, 9th, and 12th months of treatment, and conduct statistical analysis. This study will preliminarily verify the effectiveness and safety of YXFKG in the treatment of stage III hepatitis B related PLC, which may provide a new choice for clinical treatment of PLC.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 5","pages":"1127-1134"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12454270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of -deficiency constitution on cognitive impairment in cerebral small vessel disease and its neuroimaging mechanism.","authors":"Liu Xin, Yang Shuning, X U Yun","doi":"10.19852/j.cnki.jtcm.2025.05.019","DOIUrl":"10.19852/j.cnki.jtcm.2025.05.019","url":null,"abstract":"<p><strong>Objective: </strong>To explore the effect of Traditional Chinese Medicine (TCM) constitution on cognitive impairment (CI) in patients with cerebral small vessel disease (CSVD) and its underlying neuroimaging mechanism and to provide countermeasures for health management of CSVD patients.</p><p><strong>Methods: </strong>A total of 241 CSVD patients were recruited from the Department of Neurology. All subjects underwent head magnetic resonance imaging (MRI), Chinese Medicine Questionnaire (CCMQ) and cognitive function examination. The CSVD patients were divided according to the Montreal Cognitive Assessment (MoCA) score into a normal cognitive group (73 cases) and a CI group (168 cases). Logistic regression was used to analyse the risk constitution of CSVD-CI and to construct a risk prediction model. 3DT1 MRI images and FreeSurfer 6.0 software (Athinoula A. Martinos, Boston, MA, USA) were used to further explore the involvement of hippocampal subregion volume in patients with at-risk constitution and its correlation with cognitive function.</p><p><strong>Results: </strong>Logistic regression analysis showed that <i>Yang</i>-deficiency constitution (YADC) (<i>P =</i> 0.020), older age (<i>P =</i> 0.008) and hypertension (<i>P =</i> 0.011) were risk factors for CSVD-CI but that balanced constitution (<i>P =</i> 0.003) and education (<i>P <</i> 0.001) were protective factors. A receiver operating characteristic curve (ROC) was drawn, and the area under the curve was 0.820. Further comparison of overall hippocampal and 12 hippocampal subregion volumes between YADC and non-YADC patients revealed decreased total volume of the left and right hippocampus, bilateral subiculum, presubiculum, molecular layer and right fimbria in the YADC group (<i>P <</i> 0.05/13, 13 is the number of hypothesis tests). Moreover, in the YADC group, the cognitive function of CSVD patients correlated positively with the overall volume of the left hippocampus (<i>r</i> = 0.304, <i>P <</i> 0.05) and the molecular layer volume of the left hippocampus (<i>r</i> = 0.288, <i>P <</i> 0.05).</p><p><strong>Conclusion: </strong>CSVD patients with YADC are more prone towards developing CI, and asymmetric atrophy of the hippocampus might be the underlying neuroimaging mechanism. In particular, the volume of the left whole hippocampus and the left hippocampal molecular layer correlated significantly with general cognitive function.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 5","pages":"1144-1151"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12454261/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Various acupuncture therapies for managing nonspecific low back pain: a network Meta-analysis.","authors":"Guo Jixing, J I Changchun, Xie Chaoju, Rao Xiang, Sun Zhangyin, Xing Yu, Zhang Rongni, Q U Qiangqiang, Dong Youpeng, Yang Jinsheng","doi":"10.19852/j.cnki.jtcm.2025.05.002","DOIUrl":"10.19852/j.cnki.jtcm.2025.05.002","url":null,"abstract":"<p><strong>Objective: </strong>To compare the therapeutic effects of nine acupuncture-related therapies (acupuncture, scrapping therapy, catgut embedding, blood-letting, electro-acupuncture, warm acupuncture, cupping, moxibustion, and needle knife) in the treatment of nonspecific low back pain (NLBP), providing evidence-based medical support for clinical treatment.</p><p><strong>Methods: </strong>Randomized controlled trials evaluating various acupuncture therapies for NLBP were screened from the China National Knowledge Infrastructure Database, Wanfang Database, China Science and Technology Journal Database, PubMed, Web of Science, Chinese Biomedical Literature Database, Embase, and the Cochrane Library. Methodological quality was assessed using the Cochrane 5.1.0 risk of bias assessment tool, and the data were analyzed using STATA 15.1.</p><p><strong>Results: </strong>This study included 19 randomized controlled trials involving 1356 participants and assessing pain using the visual analog scale (VAS), improvement in dysfunction using the oswestry disability index (ODI), and the effective rate. Blood-letting [surface under the cumulative ranking curve (SUCRA) = 81.4] exhibited a significant clinical effect in relieving NLBP in studies with the VAS score as the outcome indicator, followed by cupping (SUCRA = 70.4) and moxibustion (SUCRA = 58.5). Scrapping therapy (SUCRA = 77.7) had the most significant effect on improving NLBP-related functional impairment, followed by moxibustion (SUCRA = 59.8), and then bloodletting (SUCRA = 58.2). The effective rates of warm acupuncture (SUCRA = 78.0), scrapping therapy (SUCRA = 77.2) and blood-letting (SUCRA = 58.6) were better than those of acupuncture (SUCRA = 12.0), especially for cold and wet NLBP. These findings demonstrate the importance of syndrome differentiation during clinical decision making when determining diagnosis and treatment.</p><p><strong>Conclusion: </strong>Blood-letting was the most effective treatment for relieving pain, and scrapping therapy was the most effective treatment for improving dysfunction. Warm acupuncture was the preferred treatment for cold and wet NLBP. However, further high-quality clinical studies are needed for validation.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 5","pages":"954-962"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12453989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on subtyping and Traditional Chinese Medicine treatment of depression based on machine learning and text mining.","authors":"Fan Mengyue, Yao Lin, Zhang Guoqing, Wang Ruixue, Chen Kexin, Fan Yujing, Wang Ziming, F U Jia, Chen Yongjun, Wang Taiyi","doi":"10.19852/j.cnki.jtcm.20250319.001","DOIUrl":"10.19852/j.cnki.jtcm.20250319.001","url":null,"abstract":"<p><strong>Objective: </strong>To research the subtyping and treatment of depression by leveraging studying on extensive Traditional Chinese Medicine (TCM) experiences through artificial intelligence (AI).</p><p><strong>Methods: </strong>We retrieved depression-related literature published from inception to April 2023 from databases. From these sources, we extracted symptoms, signs, and prescriptions associated with depression. By utilizing the tree number system in the medical subject headings (MeSH), we established a hierarchical relationship matrix for symptoms/signs, as well as depression sample fingerprints. Using an unsupervised clustering algorithm, we constructed a machine learning model for classifying depression patients. Furthermore, we conducted an analysis of medication rules for each depression cluster.</p><p><strong>Results: </strong>We created a My Structured Query Language (MySQL) database containing datasets of depression-symptoms/signs and depression-herbs, through mining 3522 published clinical literatures on TCM diagnosis and treatment for depression. We established hierarchical relationships among symptoms/signs of depression patients. Our unsupervised clustering analysis revealed that depression patients could be classified into 9 subtypes, with each subtype corresponding to a specific treatment prescription. Notably, one of the depression subtypes was consistently treated by <i>Qi</i>-tonifying formulas and herbs. This finding was further supported by data from <i>Qi</i>-deficiency patients, as there was a high similarity in the top symptoms/signs shared between this subtype and <i>Qi</i>-deficiency diagnosed by TCM.</p><p><strong>Conclusions: </strong>This study identified the subtypes and TCM treatment of depression by using machine learning and text mining.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 5","pages":"1152-1163"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12454262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hewei Jiangni recipe improved the quality of life in patients with cold-heat mixed nonerosive reflux disease: a randomized, double-blinded study.","authors":"Zhang Xiaosi, Zhang Shuangyuan, Chen Hanqing, Lin Zhengdao, Xie Chune, L I Junxiang, L I Xiaohong","doi":"10.19852/j.cnki.jtcm.20250107.001","DOIUrl":"10.19852/j.cnki.jtcm.20250107.001","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the safety and efficacy of Hewei Jiangni recipe (, HWJNR) for treating nonerosive gastroesophageal reflux (NERD) with cold-heat complex syndrome and to clarify its mechanism based on correlation analyses of intestinal flora and metabolites.</p><p><strong>Methods: </strong>Seventy-two patients with NERD and the Traditional Chinese Medicine (TCM) syndrome of intermingled heat and cold were randomly assigned to either the TCM group or the Western Medicine group, each receiving 8 weeks of treatment. The primary outcome was the score of the gastroesophageal reflux disease questionnaire (GERD-Q). Additionally, 10 healthy individuals were recruited. Mechanistic outcomes included correlation analyses of intestinal flora and metabolites in healthy individuals and NERD participants before and after treatment.</p><p><strong>Results: </strong>After 8 weeks, the effectiveness rate was 90% in the TCM group and 86.67% in the Western Medicine group (<i>P ></i>0.05). Compared with omeprazole, the TCM group significantly improved quality of life and alleviated symptoms such as loss of appetite, fatigue, bowel sounds, and coldness in the hands and feet (<i>P <</i> 0.05). Dysregulation of intestinal flora and metabolic pathways in NERD patients was restored to balance after TCM treatment, which appeared related to the TCM regulation of \"cold and heat disorders.\"</p><p><strong>Conclusion: </strong>HWJNR was clinically as effective as omeprazole and demonstrated advantages in improving quality of life.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 5","pages":"1106-1118"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12454263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Yiqi Chupi powder for alleviating cancer-related fatigue in patients following colorectal cancer surgery: a randomized controlled trial.","authors":"Hao Shulan, Nan Peng, Liu Likun, L I Xiaoli, Zhong Qiming, Gao Yu, Wang Xixing, Nie Yingfang","doi":"10.19852/j.cnki.jtcm.2025.05.017","DOIUrl":"10.19852/j.cnki.jtcm.2025.05.017","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the efficacy of Yiqi Chupi powder (, YQCPP) in reducing cancer-related fatigue (CRF) among patients with colorectal cancer (CRC) undergoing chemotherapy.</p><p><strong>Methods: </strong>This was a prospective, randomized controlled trial. Eligible patients were randomly assigned to either the experimental group, receiving a 12-week regimen of YQCPP and capecitabine plus oxaliplatin (XELOX) chemotherapy, or the control group, receiving a 12-week XELOX chemotherapy alone. Outcome measures were collected at baseline and subsequently at weeks 3, 6, 9, and 12 of the treatment period. The primary outcome was the Piper Fatigue Scale (PFS) score. The secondary outcomes were the Traditional Chinese Medicine syndrome and Karnofsky Performance Status (KPS) scores.</p><p><strong>Results: </strong>Of 84 patients initially enrolled, 78 (92.9%) were evaluable. The experimental group exhibited significantly lower PFS scores (<i>P <</i>0.05) compared to the control group across all four subscales and total scores at 6, 9, and 12 weeks (<i>P <</i> 0.05). Additionally, the experimental group achieved a significantly higher effective rate (84.0% <i>vs</i> 7.89%, <i>P <</i> 0.05). The number of patients in the experimental group with improved or stable KPS scores increased significantly over the course of the treatment period in comparison to the control group (<i>P <</i>0.05) at 6, 9, and 12 weeks. There was a significant improvement in overall quality of life at weeks 9 and 12 in the experimental group.</p><p><strong>Conclusion: </strong>YQCPP may decrease the CRF and improve quality of life in patients with CRC undergoing chemotherapy.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 5","pages":"1119-1126"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12454259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of acupuncture on central amino acid metabolism based on targeted neurotransmitter analysis in mice with inflammatory pain.","authors":"Wang Yue, Liu Xingxing, Guo Yi, Guo Yongming, Yuan Gongming, Zhang Yu, Zheng Zhiyu, X U Yuan, L I Yuan","doi":"10.19852/j.cnki.jtcm.2025.05.008","DOIUrl":"10.19852/j.cnki.jtcm.2025.05.008","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of central amino acid metabolic profiles on acupuncture analgesia.</p><p><strong>Methods: </strong>BALB/c mice were injected with Complete Freund's Adjuvant (CFA) and acupuncture was applied at Zusanli (ST36) for 7 d after modeling. Paw withdrawal thermal latency (PWTL) and paw withdrawal mechanical threshold (PWMT) were tested during the experiment. The level of prostaglandin E2 (PGE2) was measured by enzyme-linked immunosorbent assay (ELISA). Using liquid chromatography-mass spectrometry (LC-MS/MS) based on the multiple reaction monitoring (MRM)-targeted metabolomic approach, neurotransmitter in the brain and spinal cord as well as were detected, further tryptophan metabolites in spinal cord were detected, and the data was analyzed using multiquant software.</p><p><strong>Results: </strong>Acupuncture significantly improved the PWTL and PWMT on the modeling side of CFA mice, and simultaneously, decreased the PGE2 level. Based on targeted neurotransmitter analysis, acupuncture increased the expression of phenylethylamine and decreased N-acetyl serotonin in the brain, while significantly up-regulated serotonin, and down-regulated the levels of homovanillic acid, 5-hydroxy-L-tryptophan and 3,4-Dihydroxyphenylethylene Glyco in the spinal cord. Further investigation targeted tryptophan metabolism found that acupuncture considerably decreased the content of tryptophan and L-kynurenine, but increased serotonin.</p><p><strong>Conclusions: </strong>This study aims to assess changes in central neurotransmitters of CFA mice treated with acupuncture, revealing that abnormalities in central neurotransmitter metabolism may be a potential biochemical basis for pain. More importantly, our preliminary study suggests the potential role of tryptophan and its metabolites in acupuncture analgesia, which may provide new insight and direction for the pain management interface of neurometabolism.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 5","pages":"1019-1027"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12453991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the therapeutic potential of extract in targeting localized adiposity.","authors":"Choi You Yeon, Jin Seong Chul, Kim Mi Hye, Baek Hee Kyung, Kim Dong Hyun, O H Sung Hyuk, Yang Woong Mo","doi":"10.19852/j.cnki.jtcm.2025.05.004","DOIUrl":"10.19852/j.cnki.jtcm.2025.05.004","url":null,"abstract":"<p><strong>Objective: </strong>To determine direct targeting of localized adiposity through <i>Morus alba Linne</i> bark injection based on pharmacology network analysis.</p><p><strong>Methods: </strong>Male C57BL/6J mice were fed a high-fat diet (HFD) to induce obesity. After 6 weeks on HFD, the water extract of <i>Morus alba L.</i>bark (MAB, 2 mg/mL) was locally injected into one inguinal fat pad, while saline was injected into the other side, 3 times/week for 6 weeks (<i>n</i> = 6/group). The water extract of MAB was freeze-dried and then diluted in saline before use.</p><p><strong>Results: </strong>HFD-fed mice treated with local MAB topical injection showed reduced adipocyte weight and size in inguinal fat pads by dual-energy X-ray absorptiometry. No toxicity changes seen in liver, spleen, kidney tissue, or alanine aminotransferase / aspartate aminotransferase levels in serum by MAB injection. Protein levels of phosphorylated insulin receptor substrate-1 and glucose transporter type 4, and mRNA expression of adiponectin, were increased in inguinal adipose tissue injected with MAB locally. Locally MAB injection led to a decrease in glucose-6-phosphatase and phosphoenolpyruvate carboxykinase, linked to gluconeogenesis, while forkhead box protein O1, which regulates these factors, was increased. Moreover, there was an increase in adenosine 5'-monophosphate-activated protein kinase, related to lipogenesis, as well as elevated levels of hormone-sensitive lipase and fatty acid synthase, both associated with lipolysis. These results support the 'insulin signaling pathway' and 'regulation of lipolysis in adipocytes' identified in the Kyoto Encyclopedia of Genes and Genomes pathway through network analysis.</p><p><strong>Conclusion: </strong>This study suggests that MAB topical injection exhibits localized fat reduction by inhibiting insulin resistance, gluconeogenesis and lipogenesis mediator, while activating lipolysis enzymes within targeted adipose site.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 5","pages":"970-978"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12453987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}