Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan最新文献

{"title":"Paeonol's protective effects on H9C2 cells against hypoxia/ reoxygenation injury through the hypoxia-inducible factor 1-alpha/B-cell lymphoma-2/adenovirus E1B 19 kDa interacting protein 3 signaling pathway.","authors":"Zhao Chengguo, Yin Meifang, Zhou Menghan, Qin Shuzhi","doi":"10.19852/j.cnki.jtcm.2026.04.001","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.001","url":null,"abstract":"<p><strong>Objective: </strong>To elucidate the cytoprotective capacity of paeonol (PAE) against hypoxia/reoxygenation (H/R) injury in H9C2 cardiomyoblasts and to clarify its regulatory effects on the hypoxia-inducible factor 1-alpha (HIF-1α) and B-cell lymphoma-2/adenovirus E1B 19 kDa interacting protein 3 (BNIP3) signaling axis governing autophagy and apoptosis.</p><p><strong>Methods: </strong>H9C2 cells were exposed to a 12-h hypoxia condition followed by a 12-h reoxygenation regimen to simulate myocardial ischemia-reperfusion injury (MIRI). PAE pretreatment (10 μmol/L) was administered to cells before the H/R procedure. The viability of cells was determined using the cell counting kit-8 assay, whereas apoptotic rates were quantified <i>via</i> flow cytometry. Reactive oxygen species (ROS) levels were assayed by 2',7'-dichlorodihydrofluorescein diacetate fluorescence staining. Western blotting was conducted to assess protein expression, including cleaved caspase-3, and the ratio of Bcl-2-associated X protein to B-cell lymphoma 2 (Bax/Bcl-2), as well as microtubule- associated protein 1 light chain 3 (LC3) and sequestosome-1 (p62), along with components of the HIF-1α/BNIP3 signaling pathway. Autophagosomes were visualized by transmission electron microscopy. To elucidate the regulatory mechanisms, siRNA was used to knockdown HIF-1α expression, while the autophagy inhibitor 3-methyladenine (3-MA) was employed as a functional intervention.</p><p><strong>Results: </strong>PAE significantly improved cell viability following H/R treatment and inhibited apoptosis, as indicated by a reduction in cleaved caspase-3 levels and a decreased Bax/Bcl-2 ratio, along with diminished intracellular ROS levels. PAE treatment enhanced autophagosome formation and increased LC3-II levels, suggesting enhanced autophagic flux. Additionally, PAE upregulated the expression of HIF-1α and BNIP3. Silencing HIF-1α or inhibiting autophagy with 3-MA reversed the protective effects of PAE on autophagic flux, apoptosis, and cell viability.</p><p><strong>Conclusion: </strong>PAE protects H9C2 cells against H/R injury by promoting autophagy and inhibiting apoptosis, mediated through the upregulation of the HIF-1α/BNIP3 signaling pathway. These observations highlight the therapeutic implications of PAE for MIRI, particularly through targeting the HIF-1α/BNIP3 axis.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"779-787"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jianchang No. 1 (1) formula alleviates irritable bowel syndrome with diarrhea improving gut microbiota and regulating toll-like receptors/nuclear factor-kappa B signaling pathway in bowel syndrome with diarrhea rats.","authors":"Liu Yutong, Yang Yan, X U Huiying, L I Lei, G E Fei, Chen Meijuan","doi":"10.19852/j.cnki.jtcm.2026.04.009","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.009","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the therapeutic efficacy of Jianchang No. 1 (1) in treating irritable bowel syndrome with diarrhea (IBS-D) and to explore its underlying mechanisms of action.</p><p><strong>Methods: </strong>IBS-D rat models were established through a combination of chronic restraint stress, water avoidance stress, and the administration of senna decoction. Body weight, fecal water content (FWC), and intestinal capacity threshold in IBS-D rats were monitored before and after treatment. Pathological alterations in colon tissue were examined using hematoxylin and eosin staining. Serum levels of lipopolysaccharide (LPS), along with LPS content in colon tissue were measured using the enzyme-linked immunosorbent assay. 16S rRNA sequencing technology was employed to assess the abundance and compositional shifts within the gut microbiota. Quantitative real-time polymerase chain reaction, Western blot, and immunofluorescence were employed to determine gene and protein expression in colonic samples.</p><p><strong>Results: </strong>Jianchang No. 1 treatment restored body weight, lowered FWC, and raised the intestinal capacity threshold in IBS-D rats. Meanwhile, Jianchang No. 1 enhanced both the richness and composition of the gut microbiota. Moreover, Jianchang No. 1 markedly upregulated the tight-junction proteins zonula occludens-1 and occludin together with the anti-inflammatory cytokine interleukin-10, while simultaneously downregulating the LPS-toll-like receptor (TLR) 2/4- myeloid differentiation factor 88- nuclear factor kappa B (NF-κB) axis and the downstream pro-inflammatory mediators interleukin-1 beta, interleukin-6, tumor necrosis factor-alpha, together with interleukin-4 and interferon-gamma.</p><p><strong>Conclusions: </strong>Jianchang No. 1 maintains intestinal barrier integrity by reshaping gut-microbial diversity and composition, dampening the TLR/NF-κB signaling cascade, and thereby alleviating low-grade colonic inflammation and its attendant clinical symptoms in IBS-D rats.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"883-893"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yigongsan decoction ameliorates anemia of chronic disease by attenuating mothers against decapentaplegic homolog-signal transducer and activator of transcription 3 interaction to regulate iron homeostasis.","authors":"Zheng Qin, Zhu Xiaoqin, H U Jie, B U Jingyang, J I Yuting, Jiang Yiling, Shi Ling, Zhang Aiping, W U Zhihao, X U Haitao, Zhang Ruifeng, Yang Yang, Luo Meihong","doi":"10.19852/j.cnki.jtcm.2026.04.005","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.005","url":null,"abstract":"<p><strong>Objective: </strong>To investigate how Yigongsan decoction (YGS) regulates iron metabolism in anemia of chronic disease (ACD) by modulating the hepcidin-ferroportin axis, with particular focus on crosstalk between the mothers against decapentaplegic homolog (SMAD) and signal transducer and activator of transcription 3 (STAT3) signaling pathways.</p><p><strong>Methods: </strong><i>In vivo</i>: a lipopolysaccharide (LPS)-induced ACD mouse model was treated with YGS (15.413 g·kg^-1·d^-1) for 7 d. Iron metabolism was evaluated by measuring serum and splenic iron levels, as well as hepcidin antimicrobial peptide (HAMP) and ferroportin (Fpn) mRNA expression (quantitative real-time polymerase chain reaction, qRT-PCR), and protein-protein interactions (co-immunoprecipitation, Co-IP). <i>In vitro</i>: HepG2 cells were stimulated with interleukin-6 (IL-6) or activin B and treated with 10% YGS-medicated serum. Iron metabolism was evaluated by intracellular and extracellular iron. STAT3/SMAD pathway activity was assessed by siRNA knockdown, Western blotting of phosphorylated SMAD1/5/8 (p-SMAD1/5/8) and phosphorylated STAT3 (p-STAT3), and Co-IP.</p><p><strong>Results: </strong>LPS-treated mice, YGS significantly decreased splenic iron content (<i>P <</i>0.01) and increased serum iron levels (<i>P <</i>0.05). Hepatic HAMP mRNA was downregulated, while Fpn mRNA was upregulated. In HepG2 cells, YGS counteracted IL-6/activin B-induced dysregulation, reducing hepcidin and intracellular iron while increasing ferroportin and extracellular iron. siRNA experiments confirmed that YGS restored the hepcidin-ferroportin balance independently of direct SMAD or STAT3 inhibition. Co-IP and immunofluorescence revealed that YGS attenuated the competitive interaction between phosphorylated SMAD1/5/8 (p-SMAD1/5/8) and phosphorylated STAT3 (p-STAT3), reducing their nuclear co-localization and subsequent HAMP transcription.</p><p><strong>Conclusion: </strong>Our results suggest that YGS may alleviate ACD by attenuating the interaction between the SMAD and STAT3 pathways, which appears to lead to reduced STAT3 phosphorylation and hepcidin expression, thereby re-establishing iron homeostasis. This identifies YGS as a potential therapeutic agent targeting inflammation-induced iron dysregulation.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"821-831"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Biling Weitong granules on gastrointestinal motility and gastrointestinal hormones levels in rats with functional dyspepsia.","authors":"Gao Lige, L I Xueyi, Chen Feiyu, Jiang Changyu, L I Jiadi","doi":"10.19852/j.cnki.jtcm.2026.04.007","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.007","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of Biling Weitong granules on gastrointestinal motility and serum mouse motilin/human vasoactive intestinal peptide (MTL/VIP) enzyme-linked immunosorbent assay kit levels in functional dyspepsia (FD) rat models, and explore its therapeutic mechanisms against FD to provide further clinical application data.</p><p><strong>Methods: </strong>Sixty Sprague-Dawley rats were randomized into blank group (BG) and model group (MG). FD was induced in MG <i>via</i>30-d chronic unpredictable stress. After successful modeling, the model rats were divided into MG BG, Biling Weitong granules low/middle/high-dose (BL/BM/BH), and trimebutine maleate (TM) groups (<i>n =</i> 7/group) for 14-d treatments. At the end of the gavage, General parameters (body weight, food intake), gastrointestinal motility indices (gastric emptying and small intestinal propulsion rates), serum MTL/VIP levels, and gastric antrum histopathology were analyzed using hematoxylin and eosin (HE) staining.</p><p><strong>Results: </strong>After 30 d of modeling, compared with the BG group, rats in the MG group presented FD-like symptoms such as lethargy and irritability, along with significantly decreased body weight and food intake (<i>P <</i> 0.05). The gastric emptying rate and small intestinal propulsion rate were significantly reduced (<i>P <</i> 0.01), and the serum MTL content significantly decreased (<i>P <</i> 0.01). Serum VIP levels were significantly increased (<i>P <</i> 0.01). After 14 d of treatment, compared with the MG group, rats in the BL, BM, BH, and TM groups exhibited improved general condition, with significantly increased body weight and food intake (<i>P <</i> 0.05); significantly faster gastric emptying rate and small intestinal propulsion rate (<i>P <</i> 0.01); and significantly higher serum MTL content (<i>P <</i> 0.01). The serum VIP levels were significantly decreased (<i>P <</i> 0.01). There were no significant differences in body weight or food intake between the BM group and BH group (<i>P ></i> 0.05). Consistently, there were no significant differences in gastric emptying rate, small intestinal propulsion rate, or serum MTL and VIP contents between the BH and TM groups (<i>P ></i> 0.05). HE staining revealed no marked changes in the gastric antrum tissues of the BG, MG, or drug groups.</p><p><strong>Conclusion: </strong>Biling Weitong granules can modulate the levels of the gastrointestinal hormones MTL and VIP in FD rats, promote gastric emptying and small intestinal propulsion, improve the gastrointestinal motility and mental status of FD rats, and alleviate the symptoms of FD, accounting for its efficacy against FD.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"864-872"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered amino acids and acylcarnitines profile in infantile intrahepatic cholestasis liver disease: implication for Traditional Chinese Medicine syndrome differentiation.","authors":"Qiu Yanyan, Xia Yu, L I Weiwei, Yan Suqi, Pang Yusheng","doi":"10.19852/j.cnki.jtcm.2026.04.019","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.019","url":null,"abstract":"<p><strong>Objective: </strong>To explore the diagnostic efficacy of blood amino acids (AAs) and acylcarnitines (ACs) profiles as metabolic biomarkers for differentiating Traditional Chinese Medicine (TCM) syndromes in infantile intrahepatic cholestatic liver disease (IICLD).</p><p><strong>Methods: </strong>Blood samples were collected from a cohort comprising 50 healthy controls and 77 patients diagnosed with IICLD, including 43 cases categorized as cold-damp obstruction and stagnation syndrome (COS) and 34 cases as jaundice due to stasis and accumulation syndrome (JSA) based on TCM diagnostic criteria. Metabolomic analyses targeting AAs and ACs were performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).</p><p><strong>Results: </strong>Compared with the healthy controls group, the IICLD group showed 23 upregulated and 2 downregulated metabolites with a screening threshold of variables of importance in projection (VIP) ≥ 1, fold change (FC) ≥ 1.2 or ≤ 0.83, and <i>P <</i>0.05. Six different metabolites were screened between the COS group and JSA group with a screening threshold of VIP ≥ 1, FC ≥ 1.2 or ≤ 0.83, and <i>P <</i>0.05. Ornithine (Orn), Decanedioy lcarnitine (C10DC), Caproylcarnitine (C6) were identified as potential biomarkers for distinguishing between the two syndromes with VIP > 2, FC ≥ 1.2 or ≤ 0.83 and <i>P <</i>0.05; The areas under the receiver operating characteristic curves (AUC) for these biomarkers were Orn (AUC = 0.7394), C10DC (AUC = 0.7777), and C6 (AUC = 0.686). When the levels of these three biomarkers were combined, the AUC increased to 0.922.</p><p><strong>Conclusion: </strong>Distinct alteration of AAs and ACs profile highlighted the oxidative stress and mitochondrial impairment in IICLD. A combined set of metabolites (Orn+ C10DC+ C6) emerged as valuable biomarker for differentiating between the COS syndrome and JSA syndromes, providing a biochemical basis for syndrome differentiation.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"1000-1008"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of Yangxin Dawa Yimixike Mi Gao for myocardial ischemia/reperfusion injury: an integrative study of network pharmacology and and validation.","authors":"Dong Yuwei, Ran Qingzhi, Abdulla Rahima, Maitinuer Maiwulanjiang, Tian Fang, Aisa Haji Akber","doi":"10.19852/j.cnki.jtcm.2026.04.013","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.013","url":null,"abstract":"<p><strong>Objective: </strong>To systematically elucidate the cardioprotective mechanisms of the traditional Uyghur polyherbal formulation Yangxin Dawa Yimixike Mi Gao (YXDW, ) against myocardial ischemia/ reperfusion injury through integrated chemical analysis, bioinformatics prediction, and multilevel experimental validation.</p><p><strong>Methods: </strong>Using ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap-HRMS), 137 bioactive constituents were identified in YXDW. Potential targets associated with myocardial ischemia/reperfusion (I/R) injury were predicted through network pharmacology analysis and validated using Mendelian randomization (MR). Gene ontology and kyoto encyclopedia of genes and genomes enrichment analyses, together with molecular docking, were performed to identify the core regulatory pathways. An <i>in vivo</i> myocardial I/R model and an <i>in vitro</i> hypoxia/ reoxygenation (H/R) model were established in Sprague-Dawley rats and H9c2 cardiomyocytes, respectively. The therapeutic effects of YXDW were evaluated using histopathological examination, biochemical markers, including creatine kinase (CK), lactate dehydrogenase (LDH), superoxide dismutase, C-reactive protein, interleukin-6, and tumor necrosis factor-alpha, echocardiography, enzyme-linked immunosorbent assay, western blotting, and quantitative reverse transcription polymerase chain reaction.</p><p><strong>Results: </strong>UHPLC-Q-Orbitrap-HRMS fingerprinting demonstrated high batch-to-batch consistency, with similarity values greater than 0.87, and identified 23 compounds with high oral bioavailability (OB ≥ 56%). Network pharmacology and MR analyses identified 13 key targets associated with myocardial I/R injury, including C-X-C motif chemokine receptor 1 (CXCR1), mitogen-activated protein kinase kinase 1 (MAP2K1), and poly (ADP-ribose) polymerase 1 (PARP1), which may be involved in the regulation of apoptosis, oxidative stress, and inflammatory responses. <i>In vivo</i> experiments showed that YXDW administration alleviated I/R-induced myocardial injury, as evidenced by reduced serum CK and LDH levels, attenuated myocardial apoptosis, and improved left ventricular function. Furthermore, the <i>in vivo</i> and <i>in vitro</i> results showed that YXDW dose-dependently downregulated CXCR1, MAP2K1, and PARP1 expression at both the protein and messenger RNA (mRNA) levels in myocardial tissues and H/R-injured H9c2 cardiomyocytes.</p><p><strong>Conclusion: </strong>YXDW exerts cardioprotective effects against myocardial I/R injury through multi-target modulation of inflammatory, oxidative, and apoptotic pathways. Our findings provide preliminary mechanistic support for the clinical relevance of YXDW in I/R therapy and offer a modern pharmacological basis for the rational use of traditional polyherbal formulations.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"927-940"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of Dingluo nasal drops for the acute treatment of migraine: a pilot randomized trial.","authors":"Yao Yuning, A N Yuqiu, Liu Jiaojiao, D U Boxuan, Cao Kegang","doi":"10.19852/j.cnki.jtcm.2026.04.014","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.014","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the efficacy and safety of two doses of Dingluo nasal drops compared to placebo in the acute treatment of migraine.</p><p><strong>Methods: </strong>This randomized, parallel-treatment, placebo-controlled, double-blind trial was conducted at Dongzhimen hospital, Beijing University of Chinese Medicine. Eligible participants were randomly assigned (1∶1∶1) to receive either full-dose Dingluo nasal drops, half-dose Dingluo nasal drops, or a placebo for three migraine attacks. The primary endpoint was the proportion of participants achieving pain freedom at 2 h postdose, assessed in all randomly assigned participants who received study medication.</p><p><strong>Results: </strong>A total of 91 participants with 257 attacks were included in the statistical analysis. At 2 h postdose, Dingluo nasal drops were superior to placebo for freedom from pain [high dose <i>vs</i>placebo: 14.61% <i>vs</i>1.20%, <i>P</i> = 0.001, risk difference 13.40, 95% <i>CI</i>(5.70, 21.11); low dose <i>vs</i>placebo: 17.65% <i>vs</i>1.20%, <i>P <</i>0.001, risk difference: 16.44, 95% CI (8.00, 24.88)]. The high dose and the low dose effected similarly for freedom from pain [14.61% <i>vs</i>17.65%, <i>P =</i> 0.585, risk difference: －3.04, 95% <i>CI</i>(－13.97, 7.89)]. It showed a good efficacy in pain relief. The most common adverse events were nasal burning and nasal itchiness. All adverse events are transient and dissipate within a relatively short time frame.</p><p><strong>Conclusions: </strong>Dingluo nasal drops demonstrated superior efficacy to placebo in achieving pain freedom for acute migraine treatment. Mild nasal reactions were observed, with a trend toward reduced discomfort at the half-dose concentration.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"941-950"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture alleviates intestinal inflammation in rats with ulcerative colitis by regulating colonic cell autophagy and gut microbiota.","authors":"X U Huichao, Hao Jianheng, Chang Boya, Wang Danni, L I Rangqian, Cao Yuxia, Wang Haijun, J I Laixi","doi":"10.19852/j.cnki.jtcm.2026.04.010","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.010","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of electroacupuncture (EA) on autophagy and gut microbiota homeostasis in a dextran sulfate sodium (DSS)-induced rat model of ulcerative colitis (UC).</p><p><strong>Methods: </strong>UC rats received EA treatment, and efficacy was evaluated by disease activity index (DAI), colon length, serum cytokines, and histopathology. Autophagy was assessed by transmission electron microscopy (TEM), reverse transcription quantitative polymerase chain reaction, and Western blotting for autophagy-related protein (ATG)5, ATG12, microtubule-associated protein 1 light chain 3 (LC3), and sequestosome 1 (p62), with phosphatidylinositol 3-kinase/protein kinase B/ mammalian target of rapamycin (PI3K/AKT/mTOR) pathway analysis. Gut microbiota composition was profiled by 16S ribosomal RNA (rRNA) gene sequencing, and functional relevance was confirmed by fecal microbiota transplantation (FMT).</p><p><strong>Results: </strong>EA reduced DAI scores, preserved colon length, lowered tumor necrosis factor-α and interleukin-1β (IL-1β), and elevated IL-2 and IL-10. Colonic injury and inflammatory infiltration were alleviated, autophagy markers improved, and PI3K/AKT/mTOR pathway activity was suppressed. EA also enhanced microbiota diversity and composition, with FMT confirming its microbiota-autophagy-inflammation modulatory role.</p><p><strong>Conclusion: </strong>EA alleviates UC by promoting colonic epithelial autophagy through PI3K/AKT/mTOR pathway inhibition and restoring gut microbiota balance, offering a promising therapeutic strategy.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"894-906"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Berberine alleviates alcoholic liver diseaseactivating intestinal nuclear receptor subfamily 1 group D member 1.","authors":"Huang Yuwei, Cai Yuting, L I Zanjin, W U Zicong, Guo Lianxia, Lin Luomin, Dong Linlin, W U Baojian, Dong Dong","doi":"10.19852/j.cnki.jtcm.2026.04.002","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.002","url":null,"abstract":"<p><strong>Objectives: </strong>To elucidate the hepatoprotective mechanism of berberine (BBR) against alcohol-induced liver injury.</p><p><strong>Methods: </strong>Chronic and acute alcohol-induced liver injury models in mice were established to mimic human alcoholic liver disease (ALD). The therapeutic effect of BBR was evaluated in both models. The severity of liver injury, lipid metabolism and circadian clock related factors were assessed using histopathology, biochemical assays, and Western blotting. Intestinal-specific nuclear receptor subfamily 1 group D member 1 (<i>Rev-erbα</i>) knockout mice were used to validate intestinal <i>Rev-erbα</i> as the key mediator.</p><p><strong>Results: </strong>BBR alleviated hepatic steatosis and inflammation in both chronic and acute ALD models. Mechanistically, BBR activated intestinal REV-ERBα, upregulating intestinal fatty acid desaturase 2 (FADS2) expression, suppressing hepatic sterol regulatory element binding protein-1 (SREBP-1c) and its downstream lipogenic genes. These effects were abolished in intestinal specific Rev-erba knockout mice. BBR was most effective against ALD when administered during the peak expression phase of REV-ERBα (Zeitgeber Time 6).</p><p><strong>Conclusions: </strong>Intestinal REV-ERBα represents a promising therapeutic target for ALD, and chrono-modulated BBR administration may enhance treatment efficacy. This study underscores the role of gut-liver axis in ALD pathogenesis and provides a rationale for developing time-tailored therapies.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"788-797"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture enhances CD8+ T cells recruitmentmodulating sympathetic nervous system to suppress tumor growth in mice bearing triple-negative breast cancer.","authors":"Tian Yehong, Zhang Qiaoli, Zhang Rui, Gao Tao, L I Nanzhi, Huang Jinchang, Jiang Xin, Qiu Xiaowei","doi":"10.19852/j.cnki.jtcm.2026.04.006","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.006","url":null,"abstract":"<p><strong>Objective: </strong>To investigate whether peritumoral electroacupuncture (EA) suppresses tumor growth <i>via</i>CD8+ T cell infiltration mediated by sympathetic nervous system (SNS) regulation.</p><p><strong>Methods: </strong>Utilizing a mouse model of triple-negative breast cancer (TNBC), the SNS was activated through chronic restraint stress (CRS); chemical sympathetic denervation was induced by 6-hydroxydopamine (6-OHDA). The anti-tumor and immune regulatory effects of EA were evaluated by multidimensional methods, including <i>in vivo</i>bioluminescence imaging, flow cytometry, immunofluorescence, and enzyme-linked immunosorbent assay.</p><p><strong>Results: </strong>Peritumoral EA decreased the volume and weight of tumors within 22 d after the implantation. Intratumoral CD8+ T cells significantly increased after EA. C-C motif chemokine ligand 5, as a chemokine that recruits effector T cells to the tumor microenvironment, also up-regulated. Meanwhile, EA reduced intratumoral sympathetic innervation and norepinephrine level. Subsequently, we found CRS significantly accelerates tumor growth and reduces the density of CD8+ T cell infiltration. However, EA reversed the tumor-promoting effect and immune suppression caused by CRS effectively. Furthermore, chemical sympathetic ablation with 6-OHDA revealed that the anti-tumor effect of EA was dependent on sympathetic integrity, as the ablation impaired both tumor-suppressing effect and CD8+ T cell recruitment mediated by EA.</p><p><strong>Conclusion: </strong>EA enhances anti-tumor immunity through SNS emphasizing the effective remodeling of the tumor immune microenvironment in mice with TNBC by neuro-immune interactions.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"832-843"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}