Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan最新文献

{"title":"Therapeutic potential of garlic-derived exosome like nanovesicles: challenge and opportunity.","authors":"Masoumeh Amiri, Mozafar Khazaei, Tayebeh Sadat Tabatabai, Leila Rezakhani","doi":"10.19852/j.cnki.jtcm.2026.02.022","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.2026.02.022","url":null,"abstract":"<p><p>Recent advancements in nanotechnology have catalyzed interest in exosome-like nanovesicles derived from garlic, exploring their potential therapeutic applications. This review assesses the therapeutic efficacy of garlic-derived exosome-like nanovesicles (GaELNVs), emphasizing their antimicrobial, anticancer, antioxidant, and anti-inflammatory properties, alongside their utility in drug delivery systems. Synthesizing diverse studies, this review discusses methodologies for GaELNV isolation, characterization (including high-speed centrifugation, sequential centrifugation, and sucrose gradient purification), and application. GaELNVs exhibit notable therapeutic potential, evidenced by their capacity to mitigate brain inflammation, enhance cognitive function, improve glucose metabolism, and show minimal immunogenicity. These effects are attributed to their modulation of inflammatory cytokines and other molecular pathways. Moreover, GaELNVs demonstrate robust biocompatibility, stability in acidic environments, and efficient targeting capabilities. In conclusion, this review underscores the promising therapeutic applications of GaELNVs, particularly as a pioneering drug delivery system. However, further investigations are imperative to optimize clinical deployment and comprehensively elucidate their mechanisms of action.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"509-516"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qianyang Yuyin granule modulates glycolysis and inflammatory response to treat hypertensive cardiac remodeling.","authors":"Sun Zeqi, Zhang Weiting, Fan Yadong, X U Junyao, Huang Hong, Xiong Ziwen, Cao Huiting, Liu Ming, Fang Zhuyuan","doi":"10.19852/j.cnki.jtcm.20251215.001","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.20251215.001","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of Qianyang Yuyin granule (QYYY) on AngII-induced hypertensive cardiac remodeling, focusing on the role of pyruvate kinase isozyme M2 (PKM2) mediated glycolysis.</p><p><strong>Methods: </strong>A hypertensive mouse model was established in male C57BL/6 mice by continuous infusion of angiotensin II (AngII; 1000 ng·kg^-1·min^-1). Mice were administered varying doses of QYYY, with sacubitril/ valsartan (Sac/Val) serving as the positive control. Parameters evaluated included blood pressure, cardiac function, hypertrophy, fibrosis, inflammation, and apoptosis. The metabolic profile of myocardial tissue was analyzed using ultra performance liquid chromatography tandem mass spectrometry. Additionally, the involvement of the hypoxia-inducible factor 1-alpha (HIF-1α)/PKM2 signaling pathway was examined by Western blotting and immunohistochemistry.</p><p><strong>Results: </strong>QYYY significantly lowered blood pressure, attenuated cardiac hypertrophy and fibrosis, reduced serum levels of inflammatory factors tumor necrosis factor-α and tumor necrosis factor-β, and decreased activation of phospho-NF-kappa B p65 pathway in cardiac tissue of hypertensive mice. Metabolomic analysis indicated that QYYY ameliorated cardiometabolic dysfunction, primarily associated with energy and amino acid metabolism, involving modulation of the HIF-1α/PKM2-mediated glycolytic pathway.</p><p><strong>Conclusion: </strong>QYYY effectively improves cardiac remodeling in hypertensive mice, potentially through inhibition of the PKM2-mediated glycolytic signaling pathway.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"274-284"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota-mediated short-chain fatty acids contribute to the protective effects of Xiaoxuming decoction against lipopolysaccharide-induced acute lung injury.","authors":"Xiang Yijin, Yang Zhigang, Gong Shaomin, L I Xiangting, Cai Min","doi":"10.19852/j.cnki.jtcm.2026.02.002","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.2026.02.002","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the mechanisms underlying the protective effects of Xiaoxuming decoction (, XXMD) against lipopolysaccharide (LPS)-induced acute lung injury (ALI).</p><p><strong>Methods: </strong>LPS was used to construct an ALI model in mice and human pulmonary alveolar epithelial cells (HPAEpiCs). Acetate and acetate-producing bacteria, including Blautia hydrotrophica, Bacteroides thetaiotaomicron, Akkermansia muciniphila, and Bacteroides vulgatus, were detected after XXMD treatment. Zonula occludens-1 (ZO-1), occludin, phosphorylated nuclear factor-kappa B p65 (p-NF-κBp65), and NF-κBp65 levels were measured by Western blotting, and an enzyme-linked immunosorbent assay was performed to detect tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and IL-6 secretion. Transepithelial electrical resistance, fluorescein isothiocyanate-dextran, and cell counting kit-8 assays were used to detect the cell's monolayer integrity, permeability, and viability.</p><p><strong>Results: </strong>XXMD alleviated the LPS-induced downregulation of acetate and acetate-producing bacteria in feces. Furthermore, XXMD suppressed the LPS-induced downregulation of ZO-1 and occludin expression in lung tissues, LPS-induced upregulation of TNF-α, IL-1β, and IL-6 in bronchoalveolar lavage fluid, and p-NF-κBp65 levels in lung tissues. Acetate had similar effects in LPS-induced mice. Meanwhile, the seven-day survival probability was improved by XXMD or acetate treatment. Furthermore, acetate could alleviate LPS-mediated cell viability, inflammation, and permeability in HPAEpiCs, while acetate-induced effects could be abrogated by GLPG0974, a short-chain fatty acid (SCFA) receptor G-protein coupled receptor 43 antagonist.</p><p><strong>Conclusion: </strong>XXMD's therapeutic effect may be related to gut microbiota-mediated SCFAs, which alleviated LPS-induced ALI.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"285-293"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acupuncture inhibits astrocytic hyperactivationthe p38 mitogen-activated protein kinase/mitogen- and stress-activated protein kinase 1 signaling pathway in cerebral ischemia-reperfusion injury.","authors":"Zhuang Zifeng, W O Choying, Chen Hongling, Xie Hailiang, Zhao Canghuan, Zhang Di, Qing Peng","doi":"10.19852/j.cnki.jtcm.2026.02.005","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.2026.02.005","url":null,"abstract":"<p><strong>Objective: </strong>To observe the effect of acupuncture on astrocyte activation following cerebral ischemia-reperfusion injury (CIRI) <i>via</i>the p38 mitogen-activated protein kinase (p38 MAPK)/mitogen- and stress-activated protein kinase 1 (MSK1) signaling pathway.</p><p><strong>Methods: </strong>Transcriptome and single-cell sequencing were used to analyse gene expression in middle cerebral artery occlusion (MCAO)-induced rats. Acupuncture was applied at Baihui (GV20) and Dazhui (GV14) for 7 d. The infarct volume was assessed <i>via</i>2,3,5-triphenyltetrazolium chloride staining and T2-weighted imaging. Serum interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha levels were measured <i>via</i>enzyme-linked immunosorbent assay. The expression of glial fibrillary acidic protein (GFAP), complement component 3 (C3), and S100 calcium-binding protein A10 (S100A10), as well as components of the p38 MAPK/MSK1 pathway, was examined by Western blotting and immunohistochemistry.</p><p><strong>Results: </strong>CIRI activated p38 MAPK signaling, increased the expression of A1 markers (GFAP and C3), and proinflammatory cytokines, and decreased the expression of the A2 marker S100A10. Acupuncture inhibited p38 phosphorylation, upregulated MSK1, reduced C3 and inflammatory cytokines, increased S100A10 expression, decreased infarct volume, and improved neurological function.</p><p><strong>Conclusion: </strong>Acupuncture protects against ischemic stroke by targeting the p38 MAPK/MSK1 pathway. It inhibits p38 MAPK phosphorylation and activates MSK1, thereby promoting a shift in astrocyte polarization from the pro-inflammatory A1 to the reparative A2 type, as reflected by decreased C3 and increased S100A10 expression. This shift reduces pro-inflammatory cytokines, alleviates cerebral inflammation, and promotes neural repair, ultimately diminishing infarct volume, restoring neurons, and improving behavior. These findings elucidate the mechanism of acupuncture and support its clinical use.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"316-325"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077117/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Meta-analysis of Traditional Chinese Medicine constitution distribution in people with functional constipation.","authors":"Wang Xuehui, Sun Yuxin, Birling Yoann, L I Xun, Zhao Ruotong, Zheng Youyou, Song Zhenmei, Zhang Yanli, D U Hongbo, Wang Jianyun, L I Yinqing, Liu Zhaolan, Liu Jianping","doi":"10.19852/j.cnki.jtcm.2026.02.001","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.2026.02.001","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the distribution of Traditional Chinese Medicine (TCM) constitution among individuals with functional constipation, which would provide insights for developing constitution-targeted prevention and treatment strategies.</p><p><strong>Methods: </strong>A systematic search was conducted across eight databases (China National Knowledge Infrastructure Database, Wanfang Database, China Science and Technology Journal for Chinese Technical Periodicals, SinoMed, PubMed, Web of Science, Embase, and the Cochrane Library) to identify relevant observational studies assessing Traditional Chinese Medicine (TCM) constitution types among people with functional constipation. The protocol for this review has been registered on International Prospective Register of Systematic Reviews (registration number: CRD42022345996).</p><p><strong>Results: </strong>A total of sixteen cross-sectional studies involving 2976 participants were included in this review. Among functional constipation patients, the proportions of <i>Yin</i>-deficiency, <i>Yang</i>-deficiency, and <i>Qi</i>-deficiency constitution were 27.0% [95% confidence interval (<i>CI</i>) 19.9% to 36.7%], 25.2% (95% <i>CI</i> 17.5% to 35.0%) and 23.7% (95% <i>CI</i> 15.8% to 34.0%), respectively. Besides, females accounted for a higher percentage of the population with functional constipation in the included studies, and most patients were aged 45 years or older. These findings may reflect the demographic characteristics of the general functional constipation population, where older adults and women are more commonly affected. The impact of gender and age on TCM constitution distribution merits further exploration.</p><p><strong>Conclusion: </strong>Our finding revealed that <i>Yin</i>-deficiency, <i>Yang</i>-deficiency, and <i>Qi</i>-deficiency are the predominant TCM constitution types in people with functional constipation. This study suggests that clinicians should focus on patients with these TCM constitution types in the prevention and management of functional constipation. Larger sample sizes and more rigorous methodological designs are needed in future studies. Furthermore, future researches should also focus on developing individualized prevention and treatment strategies based on different TCM constitution types.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"261-273"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of body mass index on the efficacy of acupuncture treatment of postprandial distress syndrome: secondary analysis of a randomized clinical trial.","authors":"L I Yuejie, Yang Guang, Liu Luping, Yang Jingwen, Liu Cunzhi","doi":"10.19852/j.cnki.jtcm.2026.02.016","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.2026.02.016","url":null,"abstract":"<p><strong>Objective: </strong>To assess the impact of body mass index (BMI) on the effectiveness of acupuncture in treating postprandial distress syndrome (PDS).</p><p><strong>Methods: </strong>Data from 278 patients enrolled in a prior multicenter, randomized, sham-controlled trial were analyzed in this secondary study. Patients were classified into three groups according to baseline BMI: low, normal, and high. The primary outcome was the response rate after 4 weeks of treatment. The <i>χ</i>² test was used to examine the effect of BMI on acupuncture efficacy. After multiple imputation for missing data, logistic regression analysis was performed to evaluate this association. A sensitivity analysis was conducted by combining the low and normal BMI groups. In addition, BMI was analyzed as a continuous variable using logistic regression to explore its relationship with treatment efficacy in the acupuncture group.</p><p><strong>Results: </strong>The <i>χ</i>² test revealed a statistically significant difference, with the acupuncture group demonstrating a markedly higher response rate than the sham group (82.91% <i>vs</i>51.79%, <i>χ</i>² = 25.34, <i>P <</i>0.001). In both the normal and high BMI categories, the response rate in the acupuncture group (82.91%) was higher than that in the sham acupuncture group (48.21%) (<i>χ</i>² = 25.34, <i>P <</i>0.001). Following multiple imputation, logistic regression analysis demonstrated a significant overall association between BMI category and treatment response (<i>P <</i>0.001). Acupuncture showed superior efficacy compared with sham acupuncture [<i>OR =</i>1.97, 95% <i>CI</i>(1.34, 2.90), <i>P <</i>0.001]. At week 4, response rates were 68.4% in the low BMI group, 81.7% in the normal BMI group, and 96.3% in the high BMI group (<i>χ</i>²= 6.303, <i>P =</i>0.043). A significant difference was observed only between the low and high BMI groups (<i>χ</i>² = 6.717, <i>P =</i>0.010). Sensitivity analysis showed that the high BMI group achieved significantly better outcomes than the non-high BMI group in elimination rates at week 8 and week 12 (<i>χ</i>² = 4.571, <i>P =</i>0.033; <i>χ</i>² = 4.589, <i>P =</i>0.032). Logistic regression analysis indicated that BMI was an independent predictor of response rate at week 4 [<i>OR =</i>1.186, 95% <i>CI</i>(1.010, 1.394), <i>P =</i>0.038] and elimination rate at week 12 [<i>OR =</i>1.126, 95% <i>CI</i>(1.002, 1.265), <i>P =</i>0.047], whereas no significant associations were observed at other follow-up points.</p><p><strong>Conclusion: </strong>This subgroup analysis indicated that acupuncture improved PDS symptoms across different BMI categories. Acupuncture was suggested as a viable therapeutic option for patients with PDS, particularly those with a higher BMI.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"439-446"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Huiyang Shengji unguent on the NOD-like receptor family pyrin domain containing protein 3/caspase1/ gasdermin D pathway and lymphatic angiogenesis in patients with diabetic foot ulcer.","authors":"Y U Fangning, Lin Li, Tang Xiao, H E Xiujuan, Zhang Bo, Chen Yukun, M A Yizhao, Liu Zeyu, Y E Jinsheng, X U Xuying","doi":"10.19852/j.cnki.jtcm.2026.02.015","DOIUrl":"10.19852/j.cnki.jtcm.2026.02.015","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate the mechanism of Huiyang Shengji unguent (, HYSJ) for improving inflammation and promoting wound healing in patients with diabetic foot.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The primary components of the HYSJ unguent were analyzed using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A total of 20 patients with a diabetic foot wound were divided randomly into either a HYSJ treatment group (10 cases) or control group (10 cases). The HYSJ group was treated for 14 d with Hui Yang Shengji unguent, while the control group was treated for 14 d with basic fibroblast growth factor unguent. Central granulation tissue and wound secretions were collected before treatment and on the 7th and 14th day, respectively. The proteins prospero homeobox protein 1 (PROX-1), lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) and vascular endothelial growth factor receptor 3 (VEGFR-3) associated with lymphatic angiogenesis were detected by immunohistochemistry, the levels of inflammatory cytokines in wound exudates including interleukin-1β (IL-1β), interleukin-18 (IL-18), and vascular endothelial growth factor C (VEGF-C) were measured using enzyme-linked immunosorbent assay, while the protein levels of NOD-like receptor family, pyrin domain containing protein 3 (NLRP3), caspase-1, gasdermin D, and N-terminal gasdermin D were measured using immunoimprinting.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;UPLC-MS/MS analysis revealed that the high-abundance peak compounds in HYSJ unguent included coclaurine, glucoraphanin, citric acid, gallic acid, L-glutamine, and gentianose. Following treatment, there was a significant reduction in IL-1β and IL-18 levels in the HYSJ group's wound exudates on day 14 compared to pre-treatment values (&lt;i&gt;P &lt;&lt;/i&gt; 0.05). Conversely, VEGF-C levels showed a significant increase from pre-treatment levels (&lt;i&gt;P &lt;&lt;/i&gt; 0.05). In addition, the expression of PROX-1, LYVE-1 and VEGFR-3 in wound tissue increased significantly after 14 d of treatment when compared to pre-treatment levels (&lt;i&gt;P &lt;&lt;/i&gt; 0.05). Taken together, the results of these analyses provide insights into the dynamic changes of these factors in wound healing processes. On the 7th and 14th day of treatment, the expression levels of IL-β and IL-18 in wound secretions and NLRP3, caspase-1, gasdermin D, and N-terminal gasdermin D in wound tissue were significantly lower in the HYSJ group than those measured in the control group (&lt;i&gt;P &lt;&lt;/i&gt; 0.05). At the same time, the expressions of VEGF-C, PROX-1, and LYVE-1 in wound secretions in the HYSJ group were significantly higher than those in the control group (&lt;i&gt;P &lt;&lt;/i&gt; 0.05).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;The mechanism of HYSJ to promote wound healing of the &lt;i&gt;Yin&lt;/i&gt;syndrome in patients with diabetic foot may involve inhibition of cell necrosis mediated by the NLRP3/caspase-1/gasdermin D pathway, promotion of lymphangiogenesis, and establishment of a \"protective field\" ","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"427-438"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13146388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and verification of key genes related to oxidative stress in type 2 diabetes and screening of candidate drugs from Traditional Chinese Medicine.","authors":"H U Jingnan, Liao Man, X I Zhongwen, Song Jing, Wang Yining, H E Tao","doi":"10.19852/j.cnki.jtcm.2026.02.008","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.2026.02.008","url":null,"abstract":"<p><strong>Objective: </strong>To identify oxidative stress (OS)-related genes involved in type 2 diabetes mellitus (T2DM) and screen potential Traditional Chinese Medicine (TCM) candidates for therapeutic use.</p><p><strong>Methods: </strong>Gene expression data from the GSE23343 dataset were obtained from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) between healthy and T2DM patients were identified. Weighted gene co-expression network analysis (WGCNA) was performed to select modules highly correlated with clinical traits, and core genes within these modules were identified. OS-related genes were retrieved from the GeneCards database, and the overlapping DEGs, WGCNA genes, and OS-related genes were considered as hub genes in OS-related T2DM. These hub genes were validated in GSE15653 dataset. Potential TCMs were identified by mapping the hub genes to the Coremine Medical database. <i>In vivo</i>validation was performed using a T2DM rat model established by a high-fat diet and streptozotocin injection. The effects of Wedelolactone were evaluated by assessing gene expression <i>via</i>real-time quantitative reverse transcription PCR (qRT-PCR) and Western blot, alongside metabolic and liver function parameters, including fasting blood glucose, glycated serum protein, insulin resistance, and lipid profiles.</p><p><strong>Results: </strong>A total of 394 DEGs (136 up-regulated and 258 down-regulated DEGs.) were identified in the GSE23343 cohort. WGCNA results showed that the turquoise module (cor = -0.56, <i>P =</i> 0.02) and the brown module (cor = 0.66, P = 0.004) were the most correlated with T2DM. Six hub genes [interleukin 33 (IL33), S100 calcium binding protein A8 (S100A8), Golgi membrane protein 1 (GOLM1), small nuclear ribonucleoprotein U1 subunit 70 (SNRNP70), hepatocyte growth factor activator (HGFAC), and oxidative stress induced growth inhibitor 1 (OSGIN1)] were identified, with IL33, S100A8, and GOLM1 being up-regulated, and SNRNP70, HGFAC, and GOLM1 being down-regulated. These genes distinguished T2DM from healthy controls with AUC values greater than 0.8. Experimental verification using a T2DM rat model confirmed the expression patterns of the hub genes. <i>In vivo</i> data demonstrated that Wedelolactone significantly reduced oxidative stress, inflammation hepatic lipid accumulation, and improved metabolic parameters such as fasting blood glucose, glycated serum protein, and insulin resistance.</p><p><strong>Conclusion: </strong>These findings highlight the critical roles of IL33, S100A8, GOLM1, SNRNP70, HGFAC, and OSGIN1 as biomarkers in OS-related T2DM and suggest that Wedelolactone may be a promising TCM-based therapeutic candidate for T2DM.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"350-359"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tongue-coating microbiome as a predictor of solid tumors: an updated scoping review of clinical studies.","authors":"L I Kexin, Yang Jinzu, Xiao Kunlin, Duan Shaojie, Xiao Kunmin","doi":"10.19852/j.cnki.jtcm.2026.02.021","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.2026.02.021","url":null,"abstract":"<p><p>This study explores the potential of tongue coating microbiota as a non-invasive biomarker for cancer and precancerous lesions by integrating insights from multi-omics technologies and Traditional Chinese Medicine (TCM) tongue diagnosis. By bridging modern molecular research with TCM diagnostic principles, this study systematically reviewed the relationship between tongue microbiota and oncological conditions, identifying 18 eligible studies through searches in PubMed, Embase, and Web of Science. The analysis reveals significant differences in microbial diversity, abundance, metabolic pathways, and functional characteristics, which enable the partial differentiation of cancer patients from healthy individuals. However, existing research remains constrained by limited sample sizes, inconsistent analytical approaches, and a lack of integrated multi-dimensional datasets. This review highlights the promising diagnostic potential of the tongue coating microbiota in cancer detection, while suggesting that future studies should focus on standardizing methodologies and employing integrated multi-omics approaches to elucidate underlying mechanisms and advance clinical applications.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"501-508"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of Yi'anning pills for stable angina pectoris in coronary heart disease with -blood and liver-kidney deficiency syndrome: a multicenter, randomized, double-blind, superiority clinical trial.","authors":"L I Shuning, Liu Hongxu, Lai Xiaolei, Shang Juju, Xing Wenlong, Zhang Heyi","doi":"10.19852/j.cnki.jtcm.2026.02.012","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.2026.02.012","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To evaluate the efficacy and safety of Yi'anning pills for the treatment of stable angina pectoris in coronary heart disease with &lt;i&gt;Qi&lt;/i&gt;-blood deficiency syndrome and liver-kidney deficiency syndrome.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;This study was a multicenter, randomized, double-blind, double-dummy, positive-controlled clinical trial. Participants were randomly divided into the experimental group and the control group (3∶1) using the randomized block method. All participants received guideline-standardized treatment for stable angina pectoris associated with coronary heart disease, among whom the first 64 participants underwent the treadmill exercise test. The experimental group was also treated with Yi'anning pills and Xinyuan capsules simulant, while the control group received Yi'anning pills simulant and Xinyuan capsules, both for 8 weeks. The primary clinical outcome was the total Seattle Angina Questionnaire (SAQ) score, and the secondary clinical outcomes were the individual SAQ scores, treadmill exercise test results, rate of nitroglycerin cessation or reduction, classification of angina pectoris severity, and therapeutic effect against Traditional Chinese Medicine syndrome.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Overall, 158 participants were included from nine sub-centers, including 119 in the experimental group and 39 in the control group. After 8 weeks of treatment, the experimental intervention was superior to the control intervention in terms of the increase in total SAQ score (&lt;i&gt;P &lt;&lt;/i&gt;0.05). For the individual SAQ scores, the experimental intervention had an advantage over the control intervention in the reduction in physical activity limitation (&lt;i&gt;P &lt;&lt;/i&gt;0.05), the improvement in angina pectoris stability (&lt;i&gt;P &lt;&lt;/i&gt;0.05), and the frequency of angina pectoris attacks (&lt;i&gt;P &lt;&lt;/i&gt;0.05). Regarding the treadmill exercise test, the experimental intervention was superior to the control intervention for augmenting the maximum exercise heart rate (&lt;i&gt;P &gt;&lt;/i&gt; 0.05) and improving the angina-free rate after treatment (&lt;i&gt;P &gt;&lt;/i&gt; 0.05), but the differences were not statistically significant. The rate of nitroglycerin cessation or reduction was higher in the experimental group than in the control group (&lt;i&gt;P &lt;&lt;/i&gt;0.05). Regarding angina pectoris severity classification, the efficacy rate in the experimental group was higher than in the control group (&lt;i&gt;P &lt;&lt;/i&gt;0.05). Regarding the therapeutic effect against Traditional Chinese Medicine syndromes, the efficacy rates against &lt;i&gt;Qi&lt;/i&gt;-blood deficiency syndrome and liver-kidney deficiency syndrome in the experimental group were both greater than those in the control group (both &lt;i&gt;P &lt;&lt;/i&gt;0.05). There was no statistically significant difference in adverse events between the two groups (&lt;i&gt;P &gt;&lt;/i&gt; 0.05).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Based on the standardized treatment of stable angina pectoris in coronary heart disease, Yi'anning pills outperformed Xinyuan capsu","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"401-410"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}